大气PM2.5 全身暴露对大鼠多脏器病理、氧化应激指标和炎症因子的影响
合集下载
- 1、下载文档前请自行甄别文档内容的完整性，平台不提供额外的编辑、内容补充、找答案等附加服务。
- 2、"仅部分预览"的文档,不可在线预览部分如存在完整性等问题,可反馈申请退款(可完整预览的文档不适用该条件!)。
- 3、如文档侵犯您的权益，请联系客服反馈,我们会尽快为您处理(人工客服工作时间：9:00-18:30)。
生态毒理学报
Asian Journal of Ecotoxicology
第 15 卷 第 6 期 2020 年 12 月 Vol. 15, No.6 Dec. 2020
DOI: 10.7524/AJE.1673-5897.20191121001 白丽荣, 谭子康, 龚航远, 等. 大气 PM2.5 全身暴露对大鼠多脏器病理、氧化应激指标和炎症因子的影响[J]. 生态毒理学报,2020, 15(6): 132-140 Bai L R, Tan Z K, Gong H Y, et al. Effects of atmospheric fine particulate matter whole body exposure on pathology of multiple organs, oxidative stress indicators and inflammatory factors in rats [J]. Asian Journal of Ecotoxicology, 2020, 15(6): 132-140 (in Chinese)
大气 PM2. 5 全身暴露对大鼠多脏器病理、氧化应激指 标和炎症因子的影响
白丽荣, 谭子康, 龚航远, 耿红, 董川, 李瑞金*
山西大学环境科学研究所,太原 030006
收稿日期:2019-11-21 录用日期:2020-03-16
摘要: 探讨太原市冬季大气细颗粒物(PM2.5 )全身系统暴露对雄性 SD 大鼠 5 种脏器病理学、氧化应激及炎症反应的影响。 于 2018 年 11 月至 2019 年 1 月于太原市采用 IVC 通气笼对大鼠进行大气 PM2.5 的全身暴露染毒,分别染毒 1 个月和 2 个月;同 时设立相应空气过滤对照组。 采用 HE 染色方法观察大鼠肝、肾、脾、胃和小肠的组织病理学变化,并测定肝、肾、脾和胃的脏 器系数;采用生化方法测定大鼠组织匀浆液中的超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、白细胞介素-6(IL-6)和肿瘤坏 死因子-α(TNF-α)的水平。 PM2.5 暴露后大鼠 5 种组织均体现出不同程度的病理学损伤,MDA 含量与对照组相比显著增加。 PM2.5 染毒使大鼠肝和肾的脏器系数比对照组显著增高;肾、脾、胃和小肠的 SOD 活性比相应对照组明显上升;肝和小肠的 IL-
. 6A和llTNFR-iα g水h平t显s著R高e于s对er照v组e。d2.个月暴露的损伤效应比 1 个月暴露更为严重。 大气 PM2.5 全身系统暴露(结合气态污染
物)可导致大鼠多器官损害,且随着暴露时间延长而损害加重;肝、肾和小肠对 PM2.5 刺激表现敏感。 其机制可能与 PM2.5 引起 的组织 MDA 含量和炎症因子水平增加有关。 关键词: PM2.5 ;大鼠;病理学;SOD;丙二醛;炎症因子;通气笼暴露系统 文章编号: 1673-5897(2020)6-132-09 中图分类号: X171.5 文献标识码: A
Bai Lirong, Tan Zikang, Gong Hangyuan, Geng Hong, Dong Chuan, Li Ruijin*
Institute of Environmental Science, Shanxi University, an 030006, China
Received 21 November 2019 accepted 16 March 2020
第6 期
白丽荣等:大气 PM2.5 全身暴露对大鼠多脏器病理、氧化应激指标和炎症因子的影响
133
kidney, spleen, stomach and small intestine in rats were observed by hematoxylin-eosin (HE) staining, and the organ coefficients of liver, kidney, spleen and stomach were measured. The levels of superoxide dismutase (SOD), malondialdehyde (MDA), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in rat tissue homogenates were determined by biochemical methods. PM2.5 exposure caused pathological damage in varying degrees in five organs in rats, and significantly increased MDA contents compared with the corresponding controls. Organ coefficients of liver and kidney in rats exposed to PM2.5 were raised relative to the controls. The SOD activities of the kidney, spleen, stomach and small intestine were significantly higher than that of the controls. The levels of IL-6 and TNFα in the liver and small intestine were obviously elevated compared to the controls. The injury effects of 2-month exposure were more severe than that of 1-month exposure. Whole body exposure to atmospheric PM2.5(combined with gaseous pollutants) caused multiple organ damage in rats, and such injury aggravated with the prolonged exposure time. Liver, kidney and small intestine were sensitive to PM2.5 stimulation. Increases of MDA contents and inflammatory factor levels might implicate in the impairment of multiple murine organs induced by PM2.5 . Keywords: PM2.5 ; rats; pathology; SOD; malondialdehyde; inflammatory factor; Individual Ventilated Cage (IVC) exposure system
Effects of Atmospheric Fine Particulate Matter Whole Body Exposure on Pathology of Multiple Organs, Oxidative Stress Indicators and Inflammatory Factors in Rats
基金项目:国家自然科学基金资助项目(91543202,91843301);山西省自然科学基金资助项目(201801D121260) 第一作者:白丽荣(1995—),女,硕士研究生,研究方向为环境污染与健康,E-mail: 1564801337@ * 通讯作者( Corresponding author) , E-mail: lirj@
Abstract: To investigate pathology, oxidative stress and inflammatory response of whole body exposure of ambient fine particulate matter (PM2.5 ) in winter of Taiyuan in China in five organs of male SD rats in this study, rats were exposed to airborne PM2.5 using a whole-body inhalation system (individual ventilated cage (IVC) exposure system) for 1 month and 2 months respectively from November 2018 to January 2019 in Taiyuan. Meanwhile, the corresponding control groups (filter air groups) were respectively established. The histopathological changes of liver,
Asian Journal of Ecotoxicology
第 15 卷 第 6 期 2020 年 12 月 Vol. 15, No.6 Dec. 2020
DOI: 10.7524/AJE.1673-5897.20191121001 白丽荣, 谭子康, 龚航远, 等. 大气 PM2.5 全身暴露对大鼠多脏器病理、氧化应激指标和炎症因子的影响[J]. 生态毒理学报,2020, 15(6): 132-140 Bai L R, Tan Z K, Gong H Y, et al. Effects of atmospheric fine particulate matter whole body exposure on pathology of multiple organs, oxidative stress indicators and inflammatory factors in rats [J]. Asian Journal of Ecotoxicology, 2020, 15(6): 132-140 (in Chinese)
大气 PM2. 5 全身暴露对大鼠多脏器病理、氧化应激指 标和炎症因子的影响
白丽荣, 谭子康, 龚航远, 耿红, 董川, 李瑞金*
山西大学环境科学研究所,太原 030006
收稿日期:2019-11-21 录用日期:2020-03-16
摘要: 探讨太原市冬季大气细颗粒物(PM2.5 )全身系统暴露对雄性 SD 大鼠 5 种脏器病理学、氧化应激及炎症反应的影响。 于 2018 年 11 月至 2019 年 1 月于太原市采用 IVC 通气笼对大鼠进行大气 PM2.5 的全身暴露染毒,分别染毒 1 个月和 2 个月;同 时设立相应空气过滤对照组。 采用 HE 染色方法观察大鼠肝、肾、脾、胃和小肠的组织病理学变化,并测定肝、肾、脾和胃的脏 器系数;采用生化方法测定大鼠组织匀浆液中的超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、白细胞介素-6(IL-6)和肿瘤坏 死因子-α(TNF-α)的水平。 PM2.5 暴露后大鼠 5 种组织均体现出不同程度的病理学损伤,MDA 含量与对照组相比显著增加。 PM2.5 染毒使大鼠肝和肾的脏器系数比对照组显著增高;肾、脾、胃和小肠的 SOD 活性比相应对照组明显上升;肝和小肠的 IL-
. 6A和llTNFR-iα g水h平t显s著R高e于s对er照v组e。d2.个月暴露的损伤效应比 1 个月暴露更为严重。 大气 PM2.5 全身系统暴露(结合气态污染
物)可导致大鼠多器官损害,且随着暴露时间延长而损害加重;肝、肾和小肠对 PM2.5 刺激表现敏感。 其机制可能与 PM2.5 引起 的组织 MDA 含量和炎症因子水平增加有关。 关键词: PM2.5 ;大鼠;病理学;SOD;丙二醛;炎症因子;通气笼暴露系统 文章编号: 1673-5897(2020)6-132-09 中图分类号: X171.5 文献标识码: A
Bai Lirong, Tan Zikang, Gong Hangyuan, Geng Hong, Dong Chuan, Li Ruijin*
Institute of Environmental Science, Shanxi University, an 030006, China
Received 21 November 2019 accepted 16 March 2020
第6 期
白丽荣等:大气 PM2.5 全身暴露对大鼠多脏器病理、氧化应激指标和炎症因子的影响
133
kidney, spleen, stomach and small intestine in rats were observed by hematoxylin-eosin (HE) staining, and the organ coefficients of liver, kidney, spleen and stomach were measured. The levels of superoxide dismutase (SOD), malondialdehyde (MDA), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in rat tissue homogenates were determined by biochemical methods. PM2.5 exposure caused pathological damage in varying degrees in five organs in rats, and significantly increased MDA contents compared with the corresponding controls. Organ coefficients of liver and kidney in rats exposed to PM2.5 were raised relative to the controls. The SOD activities of the kidney, spleen, stomach and small intestine were significantly higher than that of the controls. The levels of IL-6 and TNFα in the liver and small intestine were obviously elevated compared to the controls. The injury effects of 2-month exposure were more severe than that of 1-month exposure. Whole body exposure to atmospheric PM2.5(combined with gaseous pollutants) caused multiple organ damage in rats, and such injury aggravated with the prolonged exposure time. Liver, kidney and small intestine were sensitive to PM2.5 stimulation. Increases of MDA contents and inflammatory factor levels might implicate in the impairment of multiple murine organs induced by PM2.5 . Keywords: PM2.5 ; rats; pathology; SOD; malondialdehyde; inflammatory factor; Individual Ventilated Cage (IVC) exposure system
Effects of Atmospheric Fine Particulate Matter Whole Body Exposure on Pathology of Multiple Organs, Oxidative Stress Indicators and Inflammatory Factors in Rats
基金项目:国家自然科学基金资助项目(91543202,91843301);山西省自然科学基金资助项目(201801D121260) 第一作者:白丽荣(1995—),女,硕士研究生,研究方向为环境污染与健康,E-mail: 1564801337@ * 通讯作者( Corresponding author) , E-mail: lirj@
Abstract: To investigate pathology, oxidative stress and inflammatory response of whole body exposure of ambient fine particulate matter (PM2.5 ) in winter of Taiyuan in China in five organs of male SD rats in this study, rats were exposed to airborne PM2.5 using a whole-body inhalation system (individual ventilated cage (IVC) exposure system) for 1 month and 2 months respectively from November 2018 to January 2019 in Taiyuan. Meanwhile, the corresponding control groups (filter air groups) were respectively established. The histopathological changes of liver,