肿瘤干细胞的分离培养及生物学特性

肿瘤干细胞的分离培养及生物学特性
王瑞海
【期刊名称】《中国组织工程研究》
【年(卷),期】2011(015)027
【摘要】背景:肿瘤干细胞是一类具有自我更新能力、不定分化潜能的种子细胞,可以形成不同分化程度的肿瘤细胞,是恶性肿瘤不断生长、复发转移、无法被传统的放化疗方法彻底杀死的根源.目的:总结肿瘤干细胞的分离培养及其生物学特性.方法:由作者采用电子检索的方式,在万方数据库中检索以"肿瘤干细胞,分离培养,生物学特性" 为中文关键词,计算机检索1990-01/2010-12 有关肿瘤干细胞的分离培养及生物学特性的文章,经检索共查到相关文献45 篇.经阅读标题、摘要、全文后,排除内容重复、普通综述、Meta 分析类文章后,筛选纳入22 篇文献进行评价.结果与结论:在肺癌、胰腺癌、结肠癌、肝细胞癌、神经系统肿瘤等多种脏器肿瘤中,CD133 被作为鉴定肿瘤干细胞的特异性标记物之一.巢蛋白、波形蛋白和CD117 在神经系统肿瘤干细胞中有表达.CD44 及内皮素转化酶在肺癌肿瘤干细胞中的表达具有意义.CD44 和CD24 可作为分选人胰腺癌细胞株PANC-1 中肿瘤干细胞的表面标志.而CD166 在大肠癌肿瘤干细胞中高表达.%BACKGROUND: Cancer stem cells are a kind of seed cells with self-renewal capacity and uncertain differe ntiation potential, and can differentiate into tumor cells to different extents, which are the root cause of continued growth, metastasis and recurrence of malignant tumors which cannot be completely killed by traditional chemotherapy.OBJECTIVE: To review the isolation, culture and biological characteristics of cancer stem cells.METHODS: A computer
retrieve of Wanfang database (1990-01/2010-12) was conducted for articles regarding the isolation,culture and biological characteristics of cancer stem cells using the keywords of “cancer stem cells, isolation and culture, biological characteristics” in Chinese. Repetitive articles, reviews and Meta analyses were excluded, and finally 22 of 45 articles were included in result analysis.RESULTS AND CONCLUSION: CD133 is regarded as a specific marker of cancer stem cells in various organ tumors, including lung cancer, pancreatic cancer, colon cancer, hepatocellular carcinoma, nervous system tumors. Nestin, vimentin, and CD117 express in cancer stem cells of the nervous system. Expressions of CD44 and endothelin-converting enzyme in lung cancer stem cells are of significance. CD44 and CD24 can be used as cancer stem cell surface markers for sorting human pancreatic cancer cell line PANC-1. CD166 has a high expression in colorectal cancer stem cells.
【总页数】4页(P5087-5090)
【作者】王瑞海
【作者单位】唐山市开平医院,河北省唐山市063000
【正文语种】中文
【中图分类】R318
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