test 121-150
初中语文 中考专题训练 阅读理解(事理说明文121-150) (含答案解析)
初中语文中考专题训练阅读理解(事理说明文121-150) (含答案解析)学校:___________姓名:___________班级:___________考号:___________一、现代文阅读头发里的秘密杨振明①一般的血液、尿液检测可反映出短时间身体讯息,相较之下,毛发记录的信息显得更加完整、也更全面。
毛发不仅含有人体DNA信息,同时还记录身体各方面的讯息,时间长达3至6个月甚至数年,即使当药物、化学物质、营养及其代谢物在体内已经消除时,这些信息在毛发中还会存在。
而且毛发中这些物质的浓度一般要大于其代谢物的浓度。
此外,头发中的药物、化学物质可能并不全是来自头发毛囊,也可以通过血液、汗液、皮脂、皮肤、空气、粉尘进入头发。
因此,我们还可以通过毛发了解详细的环境信息。
可以这样说,头发能反映人体及其生活环境的所有秘密。
②毛发检测揭示清光绪帝的死因。
清光绪帝死因,百年来一直是个谜。
检验人员根据光绪帝头发,发现并证实了光绪帝摄入体内的砒霜总量明显大于致死量。
综合以上研究成果,最终得出了光绪帝是砒霜中毒死亡的结论,清光绪帝死亡之谜得以破解。
③毛发检测广泛用于毒品检测。
由于新型毒品的科技含量越来越高,给毒品检测工作带来的难度也越来越大。
除了传统的血检和尿检外,毛发检测目前在毒品检测领域得到广泛的应用。
④红遍全球的日本偶像酒井法子曾因持有毒品被警方逮捕,酒井法子被捕以后,尿检的结果呈阴性,看不出吸毒。
警方无法以使用毒品的罪名起诉她。
不甘心的日本警方取用了酒井法子的10根头发进行检测,结果证明酒井法子确实吸毒,因而延长了她的拘留期。
⑤通过毛发检测,积极应对过劳死。
“过劳死”的说法最早来自日本,2002年,“过劳死”被《牛津英语词典》(电子版)收录。
这个单词的含义是指由于’工作压力或应激所引发的心脑血管疾病所导致的死亡。
⑥实际上,从已有医学文献来看,没有流行病学调查证据支持“过劳死”的发生与过度劳动有关联。
导致死亡的不是“过劳”,而是“过劳”诱发身体隐藏的疾病导致死亡。
可靠性测试标准
Q/.质量管理体系第三层次文件可靠性试验规范拟制:审核:批准:海锝电子科技有限公司版次:C版可靠性试验规范1. 主题内容和适用范围本档规定了可靠性试验所遵循的原则,规定了可靠性试验项目,条件和判据。
2. 可靠性试验规定根据IEC国际标准,国家标准及美国军用标准,目前设立了14个试验项目(见后目录〕。
根据本公司成品标准要求,用户要求,质量提高要求及新产品研制、工艺改进等加以全部或部分采用上述试验项目。
常规产品规定每季度做一次周期试验,试验条件及判据采用或等效采用产品标准;新产品、新工艺、用户特殊要求产品等按计划进行。
采用LTPD的抽样方法,在第一次试验不合格时,可采用追加样品抽样方法或采用筛选方法重新抽样,但无论何种方法只能重新抽样或追加一次。
若LTPD=10%,则抽22只,0收1退,追加抽样为38只,1收2退。
抽样必须在OQC检验合格成品中抽取。
3.可靠性试验判定标准。
(各电气性能的测试条件,参照器件各自的说明书所载内容)环境条件(1)标准状态标准状态是指预处理, 后续处理及试验中的环境条件。
论述如下:环境温度: 15~35℃相对湿度: 45~75%(2)判定状态判定状态是指初测及终测时的环境条件。
论述如下:环境温度: 25±3℃相对湿度: 45~75%4.试验项目。
目录高温反向偏压试验------------------------------------第4页压力蒸煮试验------------------------------------第6页正向工作寿命试验------------------------------------第7页高温储存试验------------------------------------第8页低温储存试验------------------------------------第9页温度循环试验------------------------------------第10页温度冲击试验------------------------------------第11页耐焊接热试验------------------------------------第12页可焊性度试验------------------------------------第13页拉力试验------------------------------------第14页弯曲试验------------------------------------第15页稳态湿热试验------------------------------------第16页变温变湿试验------------------------------------第17页正向冲击电流(浪涌电流)试验--------------------------第18页高温反向偏压试验一、工作原理:整流二极管在高温下加上反向偏压是一种严酷的工作方式,由于高温下漏电生产批的质量好坏。
Food Grade material
Note: 对于dry food(干性、非脂肪性食物),模拟液选用MPPO(聚(2,6-二苯基对亚苯基醚))
7
Food Grade Material Standard 食品级材料标准
2)Contact temperature 接触温度
Contact Test Temperature Temperature: T≤5 ℃ 5℃ 5 ℃ < T ≤20℃ 20℃ 20℃ < T ≤40℃ 40 ℃ 40℃ < T ≤70℃ 70 ℃ 70℃ < T ≤100℃ 100 ℃ or reflux temperature 100℃< T ≤121℃ 121 ℃* 121℃< T ≤130℃ 130℃* 130℃< T ≤150℃ 150℃* T >150℃ 175 ℃* Storage at room temperature: 40 ℃, 10day 常温下的储存罐:40度, 10天。
Textile 纺织品 Metal alloy 金属合金 Sensory Test 感官测试
Paper, baking paper 纸、烘焙纸 BfR
Plastics 塑料 BfR recommendations
Ceramic, glass, enamel陶 瓷、玻璃、搪瓷 DIN51032
PE,PP
11
Food grade material standard 食品级材料标准
French- 法国
DGCCRF (Direction générale de la concurrence, de la consommation et de la répression des fraudes ) 法国竞争、消费和抑制欺诈总理事会 Implement
新概念121-130测试
Test for Lesson 121—130Name_________ Mark__________一用下列单词的适当形式填空1.If an accident __________(happen) to you, you should report it at once to the police.2.If she__________(have) any free time, she spends it in the garden.3.If it_________(be) fine, we always walk to work.4.If it_________(be) not too far, we’ll go there on foot.5.If you __________(make) mistake, you should correct it.6.If she ________(need) any money, she will come to me for it.7.If I have time, I________(visit) the exhibition.8.If I tell the truth, no one___________(believe) me.9.If I see a nice present for her, I__________(buy) it.10.If you___________(be) ill, you should see a doctor.二单项选择1.They won’t go to the Great Wall if it______tomorrow.A.rainB.rainingC.rainsD.will rain2.We will stay at home if my aunt ______to visit us tomorrow.esB.will comeC.is cominge3.Father asked Edison______doing that for.A.how was heB.what he wasC.why was heD.where he was4.No one told us_____, so we need your help.A.how should we doB.what should we doC.how to do itD.what to do it5.Mrs Brown couldn’t find her purse. She didn’t remember____.A.whose it wasB.where it wasC.what it wasD.when it was6.Can you tell me____buy shampoo?A.where I canB.where can IC.how can ID.how I can7.Could you tell me where____now?A.you are livingB.do you liveC.you livedD.did yo live8.Schools____allow students at least one hour a day for sports.A.wouldB.mightC.shouldD.could9.I don’t know____they could pass the exam or not.A.whyB.whenC.thatD.whether10.Talking about future career, I’d prefer to be a lawyer_____.A.to being a teacherB.than a teacherC.than to be a teacherD.rather than be a teacher11.The scientist isn’t very happy with the project, and______are her supervisors.A.neitherB.eitherC.soD.as12.The iced beer tasted so good that he wouldn’t mind having_____glass.A.moreB.otherC.the otherD.another13.I was really worried about you. You____home without a word.A.mustn’t leaveB.shoudn’t have leftC.couldn’t have leftD.needn’t leave14.This book will show you_____can be used in other e\contexts.A.how what you have observedB.how you have observedC.that you have observeD.how that you have observed15. new technique______, the yields as a whole increased by twenty percent.A.working outB.having worked outC.to have been worked outD.having been worked out三完形填空In the eighteenth century, cities 1 larger and larger. People moved from the countryside and small towns to the 2 because there was 3 work for them to do in the cities.On Sundays and holidays, they like to 4 the cities and have a good time in the countryside. But not every 5 had a horse or wagon. People needed a simper means of transportation. 6 in many countries tried to solve this problem.The first bicycle, which was very simple, 7 in 1790. People called ‘the horse on wheel’. Then in 1861, after many improvements being made, the bicycle became a practical 8 of transportation.People like bikes because they weren’t as 9 as horse and didn’t need to the fed. They could go anywhere and were easy to 10.( )1.A.become B.became C.change D.changed( )2.A.cities B.countryside C.towns D.villages( )3.A.many B.much C.more D.lots( )e to B.reach C.go away D.leave( )5.A.family B.house C.home D.people( )6.A.Workers B.Inventors C.Teachers D.Drivers( )7.A.invented B.appeared C.made D.produced( )8.A.mean B.way C.means D.ways( )9.A.cheap B.easy C.difficult D.expensive( )10.A.ride B.drive C.get on D.buy四阅读理解AMr. Black comes into Class Three. The bell is ringing. He wants to teach an English lesson. Mr. White comes in and asks him to go to the office. He must go and have a meeting. Mr. Black says to his student, ‘You study by yourselves, please. I’ll be back soon.’Mr. Black comes back. He sees Mike talking to his desk mate Jack. Mr. Black is very angry.‘Stand up, Mike!’says teacher. ‘What are you doing?’‘I’m reading English now,’says Mike.‘What’s in your hand? Please look at it carefully, ’says the teacher.Mike looks at it. Oh, it’s a history book. He looks at Jack’s hand. There is an English book in his hand. He has a good idea and smiles.‘I’m sorry, Mr. Black. Jack is holding an English book and I am holding a history book. We are studying the history of English.’( )1.Mr. Black is_____teacher in the school.A.a mathsB.a historyC.an EnglishD.a physics( )2.Mr. Black comes to the office for a _____.A.dinnerB.restC.meetingD.present( )3.Mike and Jack are in fact____in class when Mr. Black comes back.A.talkingB.studyingC.learning historyD.learning English( )4.Mr. Black and Mr. White are in the same_____.A.schoolB.shopC.gradeD.classroom( )5.Jack is holding _____when Mr. Black comes back.A.an English bookB.a history bookC.a maths bookD.a physics bookBI know most English people have three names: a first name, a middle name and a family name. English people put their family names last. So the family name is the last name. I ask Jim what his full name is. He tells me that his full name is Jim Allen Green. Jim is his given name. It’s the first name. Allen is the middle name and Green is his family name. Mr. And Mrs. Green call him James. James is the name of Jim’s grandfather. But Jim’s friends usually call him Jim for short. Jim has a sister. Her full name is Catherine Doris Green. We call her Catherine Green because the middle name is not often used. Kate is short for Catherine. It is shorter and easier than Catherine. So we call her Kate more often.Chinese names are different from English name. Chinese put the family name before the given name. For instance, my name is Chen Haimin. Chen is my family name. It’s the first name. Haimin is my given name.Jim’s father is a manager. He is called Matthews Walton Green. We call him Mr. Green or Mathews. But we can’t call him Mr. Mathews. People never use Mr., Mrs. Or Miss before their first names. Jim calls my father Mr. Chen or Uncle Chen. He knows it isn’t polite for young people to call given names of old men directly in China.( )1.How many names do English people have?A.OneB.TwoC.ThreeD.Four( )2.Where do English people put their family name?A.FirstB.In the middleC.Before the given name.st( )3.If my name is Jim Doris Green, what is my given name?A.DorisB.GreenC.JimD.Doris Green( )4.Where do Chinese people put the family name?A.After the given name.B.Before the given name.C.Under the given name.D.Above the given name. ( )5.English people never use Mr. Mrs. or Miss____their first name.A.afterB.inC.onD.beforeCRose usually wakes up early in the morning. She always has her breakfast at half past six. She leaves home at about seven o’clock. She is never late for school.She has many classes in the morning. At about twelve fifteen, she comes back for lunch. In the afternoon, she isn’t so busy. After school she does her homework and plays with other children.In the evening, her parents come back from the factory. The family have supper together. After that, they usually read newspapers or listen to the radio for a little time. Sometimes, they watch TV. Rose goes to bed at half past nine. But her father often works late at night.( )1.Rose gets up____every morning.teB.earlyC.at 6:30D.at 7:00( )2.Rose often goes to school at about____.A.seven o’clockB.half past sevenC.after schoolD.six thirty( )3.Rose likes doing her homework____.A.after supperB.in the eveningC.after schoolD.after nine thirty at night( )4.Rose’s father often_____late.es homeB.has supperC.goes to workD.goes to bed( )5.They usually ____after supper.A.watch TVB.do their homeworkC.read newspapersD.play with other children五改错The Internet is playing a important part in 1.____________our daily life. On the net, we can learn about 2.____________news both home and abroad and some other 3._____________informations as well. We can also make phone calls. 4.____________send messages by e-mails, go to net schools, and 5.____________learn foreign languages by ourselves. Beside, we 6._____________can enjoy music, watch sports matches, and play the 7._____________chess or cards. The net even help us do shopping, 8._____________make a chat with others and make friends with them. 9._____________In a word, the Internet has made our life more easier. 10._____________六写作以‘A birthday party’为题,写一篇给你留下深刻印象的一次生日派对,或者写一篇你梦想中的生日派对。
怀孕280天之121-150
饮食营养 据估计整个孕期需要1000毫克铁,其中350毫克用于满足胎儿和胎盘的需要,450毫克用于增加血容量的需要,其余200毫克贮存起来以便作为分娩时血容量减少的铁库。妊娠期孕妇对铁的吸收率可以增加2~3倍,并且停止月经可以减少铁的损失。日本在妊娠前半期加3毫克,后半期加8毫克;美国不分前后期每日加15毫克,总量达30毫克/天。这一水平通常不能从食物中获得,建议孕妇在怀孕12周开始补充30毫克铁/天。我国的营养素供给量为妊娠中后期每天加10毫克,达到28毫克/天.
怀孕126天
宝宝发育 胎儿现在长度为180毫米左右,大到足以放在你的手掌上。宝宝一直都在不停地动作着。现在别指望宝宝会拳打脚踢,现在的感觉更像是肚子在咕咕叫,水泡在破裂,或是像消化不良或饥饿的感觉。
胎教趣闻 母亲还可采用给胎儿唱歌的胎教方法。著名小提琴家叶胡迪?梅纽因在1980年12月29日英国胎儿心理学会的成立大会上说,他的母亲在他出生前经常对他唱歌,可能有助于培养他杰出的音乐才能。孕妇给胎儿唱歌是一种自然的胎教,母亲声音的自然振动,母亲的歌声可带给胎儿和谐的感觉和情绪上的安宁感。母亲富有节奏的心脏搏动声,是胎儿所处环境中最先听到的声音,如母亲心率节奏正常,胎儿就会感到一切正常,就会感到环境安全而无忧无虑。母亲唱歌时,歌声与她的呼吸、心跳、胸腔和腹部的运动是一致的。胎儿更喜欢母亲唱的歌声,更能直接地刺激胎儿的听觉,促使胎儿的神经系统和感觉器官的发育,促进胎儿的记忆发展。
准妈妈保健 感染、贫血、甲状腺机能亢进、多胎妊娠等,或有产科合并症,如习惯性流产、妊娠合并高血压、妊娠期流血、有早产史等,不宜进行运天气热易流汗、盐分流失多;再者,孕妇体质较燥热,神经内分泌增加,容易食欲不振,或者孕妇吃的食物虽然精致,但是并不均衡,从而有营养不良的状况,所以食补中可把握住清淡原则。如:新鲜果汁(橙汁、苹果汁、柠檬汁、西红柿、葡萄汁、菠萝汁等),含有极丰富的营养价值,如维生素、矿物质;另外像冬瓜、红枣、荷叶、茯苓、扁豆、莲子等也是很好的食材,是非常适合夏季凉补的食物,可将其制成红枣茶、冰糖莲子、冬瓜蛤蛎、荷叶排骨。
中考课外文言文阅读训练150篇121-125(附答案与译文)
中考课外文言文阅读训练150篇121-125(附答案与译文)121焚猪验尸张举,吴①人也,为句章②令。
邑有妻杀夫者,因放火烧舍,称“火烧夫死”。
夫家疑之,诉于官。
妻不服。
举乃取猪二口,一杀之,一活之,而积薪焚之,活者口中有灰,杀者口中无灰。
因验尸,口果无灰也,鞠③之服罪。
(宋·郑克《折狱龟鉴·证匿》)【注释】①吴:三国时的吴国。
②句(gōu)章:古县名,故址在今浙江余姚。
③鞠(jū):审问犯人。
【阅读训练】一、解释下列各句中加着重号的词语。
A夫家疑.之() B诉.于官()C因放火烧舍.() D积薪.焚之()二、下列各句中加着重号的“为”字的意义和用法与例句相同的一项是()例句:为.句章令A问泉哪的清如许?为.有源头活水来。
B此中人语云:“不足为.外人道也!”C孤岂欲卿治经为.博士也 D舌一吐而二虫尽为.所吞。
三、下例各句中加中加着重号词语的词用法与例句不同的一项是()例句:一杀之,一活.之。
A山不在高,有仙则名.。
B故自号.曰醉翁也。
C虽人有百口,口有百舌,不能名.其一处。
D妇抚儿乳,儿含乳.啼。
四、把下列句子翻译为现代汉语。
①夫家疑之,诉于官。
妻不服。
译文:②因验尸,口果无灰也,鞠之服罪。
译文:五、读过这篇短文后,你受到什么启发?请用简要的话回答出来。
答【参考答案】一、A怀疑。
B控告。
C房屋。
D柴。
二、与例句相同的是C,例句与C项都是动词,做,担任。
A连词,因为。
B介词,向。
D介词,被。
三、与例句不同的是D,乳是名词,乳头。
例句与ABC三项都是名词用作动词。
例句:活:使……活,不杀。
A名:出名,有名。
B号:取号。
C名:说出。
四、①丈夫家的人对此事感到怀疑,便向官府控告(这个女人),这个女人不承认。
②然后检验男人的尸体,嘴里果然没有灰。
(再来)审问这个女人,(她只好)认罪。
五、示例:对于不能直接判断的事情,可以换个角度去思考,通过别的途径来寻求解决问题的方法。
【参考译文】张举是吴国人,做句章县的县令。
2020年公考申论必知的时政热词(121-150)
2020年公考申论必知的时政热词(121-150)121.四项基本原则答:坚持社会主义道路、坚持人民民主专政、坚持中国共产党的领导、坚持马克思列宁主义毛泽东思想。
122.“四有”新一代革命军人答:有灵魂、有本事、有血性、有品德的新一代革命军人。
123.“四个有利于”答:多推有利于增添经济发展动力的改革,多推有利于促进社会公平正义的改革,多推有利于增强人民群众获得感的改革,多推有利于调动广大干部群众积极性的改革。
124.四个依法答:党委依法决策、机关依法指导、部队依法行动、官兵依法履职。
125.监督执纪“四种形态”答:一、党内关系要正常化,批评和自我批评要经常开展,让咬耳扯袖、红脸出汗成为常态;二、党纪轻处分和组织处理要成为大多数;三、对严重违纪的重处分、作出重大职务调整应当是少数;四、严重违纪涉嫌违法立案审查的只能是极极少数。
126.党内政治生活“四性”答:政治性、时代性、原则性、战斗性。
127.第四代移动通信答:第四代移动电话行动通信标准,指的是第四代移动通信技术,外语缩写:4G。
128.五位一体答:指经济建设、政治建设、文化建设、社会建设、生态文明建设。
129.五大发展理念答:创新、协调、绿色、开放、共享。
130.“五大思维”能力答:战略思维、创新思维、辩证思维、法治思维、底线思维。
131.“五个严禁”答:最高法院于2009年1月8日推出的规定。
即严禁接受案件当事人及相关人员的请客送礼;严禁违反规定与律师进行不正当交往;严禁插手过问他人办理的案件;严禁在委托评估、拍卖等活动中徇私舞弊;严禁泄露审判工作秘密。
132.“五个过硬”按照政治过硬、业务过硬、素质过硬、纪律过硬、作风过硬的要求,努力建设一支信念坚定、执法为民、敢于担当、清正廉洁的政法队伍。
133.“五大战区”答:东部战区、南部战区、西部战区、北部战区、中部战区。
134.“五险一金”答:五险一金是指用人单位给予劳动者的几种保障性待遇的合称,包括养老保险、医疗保险、失业保险、工伤保险和生育保险,及住房公积金。
生化试剂参数
可不选择设定。副波长可消除样本溶血,血清浑浊的干扰影响,副波 长与主波长越接近越好。
检测时吸光度值为主波长的吸光度减去副波长的吸光度。
生化试剂主要参数及意义
分析时间:
孵育时间(incubate
time)
一点终点法的孵育时间是指样本与试剂开始到反应终点的时间;两点 终点法是指第一个吸光度反应点开始到第二个吸光度点为止时间。一 般为5min。
生化试剂主要参数及意义
反应方向(response direction)
正向反应(UP/INC/+):吸光度增加。
负向反应(DOWN/DEC/-):吸光度下降。
测定温度 30℃、37℃、25℃ 样本体积(sample volum) 一般为2-35μl ,以0.1μ l步进。 试剂量(regengt volum) 一般为20-300μ l ,以1μ l步进。 不同机型生化分析仪的样本量及试剂量限制不同,样本与试剂可根据比例进行增 大或减少。
淡蓝色字体标注参数设定时一般为机器默认。
各机型生化分析仪概括介绍
AU400 AU2700
AU640
AU480
各机型生化分析仪概括介绍
试剂位 R1/R2 项目 ( 双) 速度 无 ISE 样本 (ul) R1 (ul) R2 (ul) 反应液 (ul)
机型
AU
系 列
AU400 AU600 AU640 AU2700 AU5600
生化试剂其他参数及意义
反应过程吸光度界限(Absorbance Limit) 在动力学反应中,由于高浓度(活性)样本使底物耗尽,所测吸光度已 不可靠,使高浓度的样本测定值低很多,甚至为负值。为能正确反映测定 结果,需设置底物耗尽限(某一特定吸光度),是在反应时间内反应没有 发生底物耗尽时所能达到的最小(反应曲线向下)或最大(反应曲线向上) 的吸光度值 ,该吸光度即为吸光度界限。可用于确保高浓度(活性) 样本结果的准确性。
张景岳古方八阵121-150
编号121 (仲景小青龙汤八)方剂组成麻黄去节, 桂枝, 芍药, 甘草, 细辛, 干姜, 各三两. 半夏, 五味, 各半升主治功效治伤寒表不解, 心下有水气, 呕哕而欬, 发热, 或渴, 或利, 或小水不利, 小腹满而喘, 并治肺经受寒, 欬嗽喘急, 宜服此以发散表邪作者见解右八味, 以水一斗, 先煮麻黄减二升, 去上沫, 内诸药, 煮取三升. 温服一升. 按: 右方乃仲景古法, 今当随证轻重, 酌宜用之编号122 (仲景桂枝汤九)方剂组成桂枝, 芍药, 生姜, 各三两. 甘草, 二两. 大枣, 十二枚主治功效治太阳经伤风, 发热, 自汗, 恶风作者见解右以水七升, 微火煮取三升, 去滓. 适寒温服一升. 服已须臾, 食热稀粥一升余以助药力, 温覆一时许, 令遍身微似有汗者佳, 不可令如水流漓, 病必不除编号123 (桂枝加黄耆汤十)方剂组成即前桂枝汤加黄耆二两主治功效治黄疸脉浮者, 当以汗解之作者见解编号124 (桂枝加大黄汤十一)方剂组成即前桂枝汤内加大黄一两主治功效作者见解编号125 (栝蒌桂枝汤十二)方剂组成即前桂枝汤加栝蒌根二两主治功效治痉作者见解编号126 (仲景桂枝人参汤十三)方剂组成桂枝去皮, 炙甘草, 各四两. 白朮, 人参, 干姜, 各三两主治功效治太阳伤寒, 表里不解, 胁热下利者作者见解右五味, 以水九升, 先煮四味取五升, 后内桂枝, 更煮取三升. 温服一升, 日再夜一服编号127(仲景桂枝麻黄各半汤十四)方剂组成桂枝去皮, 麻黄去节, 芍药, 甘草炙, 生姜切, 各一两. 大枣, 四枚, 擘. 杏仁, 二十四个, 汤浸, 去皮尖主治功效治太阳伤寒如疟状, 发热恶寒, 不能得汗, 热多寒少而身痒者作者见解右七味, 以水五升, 先煮麻黄一, 二沸, 去上沫, 内诸药, 煮取一升八合, 去滓. 温服六合编号128 (桂枝附子汤十五)方剂组成方在热阵三十主治功效治伤寒风湿身痛作者见解编号129 (桂枝甘草汤十六)方剂组成方在热阵四四主治功效治过汗心悸作者见解编号130 (桂枝葛根汤十七)方剂组成方在痘疹三七主治功效解散寒邪作者见解编号131 (仲景柴胡桂枝汤十八)方剂组成柴胡四两. 桂枝去皮, 人参, 黄芩, 芍药, 生姜, 各一两半. 甘草炙, 一两. 半夏, 二合半. 大枣, 六枚, 擘主治功效治伤寒发热, 微恶寒, 支节烦疼, 微呕, 心下支结, 外证未去者作者见解右九味, 以水七升, 煮取三升, 去滓. 温服编号132 (仲景小柴胡汤十九)方剂组成柴胡, 半斤. 半夏, 半升. 人参, 黄芩, 生姜, 甘草, 各三两. 大枣, 十二枚, 擘主治功效治邪在肝胆半表半里之间, 寒热往来, 喜呕, 或日晡发热, 胁痛耳聋, 郁怒痎疟等证作者见解右七味, 以水一斗二升, 煮取六升, 去滓, 再煎取三升. 温服一升, 日三服. 若胸中烦而不呕, 去半夏, 人参, 加栝蒌实一枚. 若渴者, 去半夏, 加人参合前成四两半, 栝蒌根四两. 若腹中痛者, 去黄芩, 加芍药三两. 若胁下痞, 去大枣, 加牡蛎四两. 若心下悸, 小便不利者, 去黄芩, 加茯苓四两. 若不渴, 外有微热者, 去人参, 加桂三两, 温覆取微汗愈. 若欬者, 去人参, 大枣, 生姜, 加五味子半升, 干姜二两编号133 (薛氏加味小柴胡汤二十)方剂组成即前方加丹皮, 栀子主治功效亦名柴胡栀子散. 治乳母肝火发热, 致儿为患, 及风热生痰等证作者见解编号134 (《良方》加味小柴胡汤二一)方剂组成即前方小柴胡汤加枳壳面炒, 牡蛎粉. 加姜三片, 枣二枚主治功效治伤寒胁痛, 及少阳厥阴热疟作者见解水二钟, 煎服编号135 (加减小柴胡汤二二)方剂组成即前小柴胡汤去黄芩, 加芍药. 君姜, 枣主治功效治脉弦, 寒热, 腹中痛作者见解水煎服编号136 (柴胡石膏汤二三)方剂组成柴胡, 二钱. 石膏, 三钱. 甘草, 一钱主治功效治少阳阳明外感挟火, 头痛口干, 身热恶寒拘急作者见解右用姜, 水煎服. 气虚者加人参编号137(大柴胡汤二四)方剂组成方在攻阵七主治功效治表证未除, 里证又急, 汗下兼行, 宜此作者见解编号138 (柴平汤二五)方剂组成方在和阵二三三主治功效治湿疟一身尽痛作者见解编号139 (柴苓汤二六)方剂组成方在和阵一九二主治功效治身热烦渴泄泻作者见解编号140 (加减柴苓汤二七)方剂组成方在和阵一九三主治功效治诸疝. 和肝肾, 顺气除湿作者见解编号141 (仲景四逆散二八)方剂组成柴胡, 芍药, 甘草, 枳壳, 各等分主治功效治阳气亢极, 血脉不通, 四肢厥逆, 在臂胫之下者. 若是阴证, 则上过于肘, 下过于膝, 以此为辨, 乃不当用此也作者见解为细末. 每服二钱, 米饮调下, 日三服. 嗽加五味子, 干姜各五分. 悸者, 加桂五分. 腹痛, 加附子一枚炮令坼. 泄利下重者, 浓煎韭白汤调服编号142 (仲景葛根汤二九)方剂组成此即桂枝汤加麻黄, 葛根也. 葛根, 四两. 麻黄去节, 生姜, 各三两. 桂去皮, 芍药, 甘草炙, 各二两. 大枣, 十二枚, 擘主治功效治太阳阳寒, 项背强几几, 无汗恶风, 及太阳阳明合病下利者作者见解右七味, B3咀. 以水一斗, 先煮麻黄, 葛根减二升, 去沫, 内诸药, 煮取三升. 去滓, 温服一升, 覆取微似汗, 不须啜粥, 余如桂枝法将息及禁忌编号143 (升麻葛根汤三十)方剂组成升麻, 葛根, 芍药, 甘草, 各等分主治功效治伤寒阳明经证, 目痛鼻干不眠, 无汗恶寒发热, 及小儿疮疹疫疠等证作者见解水二钟, 煎一钟. 寒多热服, 热多温服编号144 (柴葛解肌汤三一)方剂组成柴胡, 干葛, 甘草, 黄芩, 芍药, 羌活, 白芷, 桔梗主治功效此《槌法》加减方. 治足阳明证, 目痛鼻干不眠, 头疼, 眼眶痛, 脉微洪者作者见解水二钟, 姜三片, 枣二枚. 《槌法》加石膏末一钱煎之, 热服. 本经无汗恶寒者去黄芩, 冬月加麻黄, 他时加苏叶编号145 (葛根葱白汤三二)方剂组成葛根, 芍药, 川芎, 知母, 各二钱. 生姜, 二钱. 葱白, 五寸主治功效治伤寒已汗未汗, 头痛作者见解水二钟, 煎一钟服编号146 (连须葱白汤三三)方剂组成连须葱白切, 半片. 生姜, 二两主治功效治伤寒已汗未汗, 头痛如破作者见解水三钟, 煎一钟半, 分二服编号147(《局方》参苏饮三四)方剂组成人参, 苏叶, 干葛, 前胡, 陈皮, 枳壳, 半夏, 茯苓, 各八分. 木香, 桔梗, 甘草, 各五分主治功效治四时感冒伤寒, 头痛发热, 恶寒无汗, 及伤风欬嗽声重, 涕唾稠粘, 潮热往来. 此药解肌宽中, 孕妇伤寒, 痘疹并治作者见解水二钟, 姜五片, 枣一枚, 煎八分. 热服编号148 (加减参苏饮三五)方剂组成方在痘疹三四主治功效治痘疹初热见点, 解利之药作者见解编号149 (败毒散三六)方剂组成人参, 茯苓, 枳壳, 甘草, 川芎, 羌活, 独活, 前胡, 柴胡, 桔梗, 各等分主治功效亦名人参败毒散. 治四时伤寒瘟疫, 憎寒壮热, 风湿风眩项强, 身体疼痛, 不问老少皆可服. 或岭南烟瘴之地, 疫疠时行, 或处卑湿, 脚气痿弱等证, 此药不可缺, 日三服, 以效为度作者见解水一钟半, 姜三片, 煎服. 或为细末, 沸汤点服编号150 (加味败毒散三七)方剂组成方在外科四一主治功效解利足三阳热毒, 寒热如疟作者见解。
电感全型号尺寸封装
1.63
0.26
HSM32-820K 82.0
1.82
0.23
HSM32-101K 100.0
2.41
0.20
HSM32-121K 120.0
2.72
0.18
HSM32-151K 150.0
3.12
0.16
HSM32-181K 180.0
3.67
0.15
HSM32-221K 220.0
4.99
0.14
5-21
HSMLFQ321618/322520/453226/575047 TYPE HSM0402F/0802F/0804F/0810F/1206F/ TYPE HSM0504/0904/1105/1306/HC TYPE
22-28 29-36 37-41
HSMRH62B/64B/73/74/124/125/127 TYPE HSMRH2D/3D/4D/5D/6D/8D TYPE
开磁路设计具有大功率 .高饱和电流 ..低阻抗 .小型化之特点。
APPLICATIONS( 用途 )
Power supply for vtr,OA equipment Digiht camera ,LCD television set notebook PC,portable
communication
→EXTERNAL DIMENSION UNIT:mm( 外形尺寸 )
PART NO.
A
B
C
H
I
J
HSM31A
3.5± 0.3 3.0
±0.2 1.1
±0.3
3.5 1.6 0.8
HSM31B HSM32
3.5± 0.3 3.0 3.5± 0.3 3.0
呼衰121-150
1、吸呼比中较长的吸气时间产生的影响说法错误的是 DA.可导致气体陷闭B.可导致A uto-PEEPC.可减少心输出量D.减少动脉氧合E.增加平均气道压2、关于吸呼比说法错误的是 DA.一般1:1.5~2B.阻塞性通气障碍可调至1:3或更长的呼气时间C.限制性通气障碍可调至1:1D 对于自主呼吸的患者,用较长的吸气时间(>1S)比较理想3、鼻导管吸入的氧流量为2 L/min,其吸入氧浓度为:CA 21%B 25%C 29%D 33%E 37%4、下列哪项不是机械通气的相对禁忌证:(没有绝对禁忌证)( E)A.气胸及纵隔气肿未行引流者B.伴肺大疱者C.低血容量性休克未纠正者D.严重肺出血;E.多发性肌炎患者5、评价气管插管插入位置错误的是( E)A.手控人工通气听诊两肺呼吸音,与插管前听诊情况对比B.呼出气CO2监测C.连接麻醉机观察呼吸囊有无扩张和收缩D.拍X线胸片E.荧光探条不能作为评价手段6、不属于更换气管插管的适应症有( E)A.气囊漏气或破裂B.插管保留后发生呼吸道炎症,需引流分泌物C.导管被痰痂或粘液栓堵住D.需重置较粗导管以减低过高气流阻力E.导管已使用1天后7、不属于机械通气的作用(B)A.控制和调节呼吸B.可替代心肺复苏术C .改善通气换气功能D .减低呼吸作功E. 纠正病理性呼吸动作8、机械通气临床治疗目的说法不全面的是(B)A.纠正低氧血症B. 降低二氧化碳蓄积C .减少全身和呼吸功氧耗D .预防和治疗肺不张E.维持胸壁稳定性9、下列哪项不是机械通气的适应症(E)A.急.慢性呼衰B.呼吸肌无力C.严重肺水肿。
D.外科手术后。
E.大咯血10、下列哪项不是机械通气相对禁忌证:(没有绝对禁忌证)( B )A.严重肺大泡和未引流的气胸B.脊柱侧弯后凸C .支气管胸膜瘘D. 低血容量性休克未纠正者E .肺组织无功能:毁损肺11、下列哪项呼吸机与病人的连接方式有使用方便,可避免胃肠胀气,利于吸痰的特点(C)A.接口和鼻夹B.封闭式面罩C.喉罩D.经口或鼻气管插管E.气管切开插管12、下列哪项不属于机械通气中吸气向呼气切换的常用方式(C)A.压力切换B.容量切换C.人工切换D.流速切换E.时间切换13、易发生通气过度或不足,自主呼吸与呼吸机不同步的通气模式是( C )A.分钟指令通气B.持续气道正压通气C控制通气D.间歇指令和同步间歇指令E.双相气道正压通气14、降低气道压力的方法错误的是(B)A. 消除原因:堵塞.缺氧.管道扭曲B. 降低呼吸频率C.延长吸气时间D.使用解痉平喘药物E.人工冬眠.肌松药15、对心血管系统有抑制作用,降低血压和心输出量的通气模式是( B)A.分钟指令通气B.持续气道正压通气C.间歇指令和同步间歇指令D辅助/控制通气E.双相气道正压通气16、久用易致呼吸机萎缩,辅助频率过快时可致A uto-PEEP的通气模式是( D)A.分钟指令通气B.持续气道正压通气C.间歇指令和同步间歇指令D辅助/控制通气E.双相气道正压通气17、频率设置过慢可致疲劳、自主呼吸急促和高碳酸血症的通气模式是( C)A.分钟指令通气B.持续气道正压通气C.间歇指令和同步间歇指令D辅助/控制通气E.双相气道正压通气18、呼吸浅快者可致有效通气量不足的通气模式是( A)A.分钟指令通气B.持续气道正压通气C.间歇指令和同步间歇指令D辅助/控制通气E.双相气道正压通气19、提供的机械辅助功较低,需要患者有较稳定的自主呼吸的通气模式是( E)A.分钟指令通气B.持续气道正压通气C.间歇指令和同步间歇指令D辅助/控制通气E.双相气道正压通气20、下列哪项不属于机械通气中呼气向吸气切换的常用方式(B)A.自主切换B.容量切换C.人工切换D.时间切换21、连接模拟肺时,面板报警区始终有报警,其原因中错误的是(B)A.病人管路有漏气B.湿化器储水过多C.积水杯有积水。
高中英语词汇手册page121-150配套翻译
P121-1501. 面临危险时我们必须保持冷静(face)Facing danger/ Faced with danger /When facing danger, we must keep calm.2. 我们最好不要与言而无信的人打交道。
(fail)We’d better not make friends with those who always fail to keep their promises.3. 龙金茶不仅在国内享有盛誉,许多外国人也熟悉它。
(familiar)Longjing tea not only enjoys a high reputation at home but is familiar to many foreigners.4. 他作为一个杰出的科学家而闻名,但他却淡泊名利。
(famous)He is famous as an outstanding scientist, but he isn’t interested in fame and wealth.5. 他在演讲比赛中的表现远不令人满意。
( far from)His performance in the speech competition is far from satisfactory.6. 她被这件时髦的外套吸引住了。
(fascinate)She was fascinated by the coat in fashion.7. 格林先生说他赞成他的儿子参加学校的篮球队.(in favor of)Mr. Green said that he was in favor of his son (‘s) joining the school football team.8. 我不敢到那里去, 因为怕被他看见.(fear)I dare not go there for fear of him seeing me/ that he will see me9. 人们发现越来越难跟上现代科学的发展。
新四级核心700词(121-150)
新四级核心700词(121-150)121. acid n. 酸,酸性物质 a. 酸的;尖刻的122. acknowledge v. 承认;致谢123. balcony n. 阳台124. calculate vt. 计算,核算125. calendar n. 日历,月历126. optimistic a. 乐观127. optional a. 可以任选的,非强制的128. outstanding a. 杰出的,突出的,显著的129. export n. 出口(物) v. 出口,输出130. import n. 进口(物) v. 进口,输入131. impose vt. 把……加强(on);采用,利用132. religion n. 宗教,宗教信仰133. religious a. 宗教的134. victim n. 牺牲品,受害者135. video n. 电视,视频 a. 电视的,录像的136. videotape n. 录像磁带 v. 把……录在录像带上137. offend v. 冒犯,触犯138. bother v. 打搅,麻烦139. interfere v. 干涉,干扰,妨碍140. internal a. 内部的,国内的141. beforehand ad. 预先,事先142. racial a. 人种的种族的143. radiation n. 放射物,辐射144. radical a.根本的;激进的145. range n. 幅度,范围 v. (在某范围内)变动146. wonder n. 惊奇,奇迹 v. 想知道,对……感到疑惑147. isolate vt. 使隔离,使孤立148. issue n. 问题,争论点;发行,(报刊)一期149. hollow a. 空的,中空的,空虚道150. hook n. 钩 vt. 钩住【。
ACAA数字图像工程师121-150
ACAA数字图像工程师121-1501. 以下哪个菜单命令可以查看溢色? [单选题] *A.选择〉色彩范围(正确答案)B.图像〉调整〉可选颜色C.图像〉调整〉替换颜色2. 下列关于习惯工具取样点的描述正确的是: *A.单个像素取样的取样点(正确答案)B.3*3 平均的取样(正确答案)C.101*101 平均的取样(正确答案)D.自定义大小平均的取样3. 128下列哪些图像颜色模式是通过“新建”文件对话框无法实现的? *1. Lab 模式2. 多通道模式(正确答案)3. 索引色模式(正确答案)4. 位图模式4. 在对一幅人物图象执行了模糊、杂点等多个滤镜效果后,如果想恢复人物图象中局部,如脸部的原来样貌,下面可行的方法: *A.采用仿制图章工具B.配合历史记录调板使用橡皮工具(勾选工具选项栏中的“抹到历史记录”选项)(正确答案)C.配合历史记录调板使用历史记录画笔(正确答案)D.使用菜单中的重做或后退的命令5. 如图所示:图中上方的红色文字要改变颜色成橙色,下列方法描述正确的是:*A.使用工具选项栏中的设置文本颜色按钮挑选颜色(正确答案)B.将前景色设置成橙色,填充前景色(正确答案)C.将前景色设置成橙色,使用油漆桶工具填充D.使用文字工具将需要改变颜色的文本选择,单击设置前景色按钮挑选颜色(正确答案)6. 如图所示,将左图图层内的老者填充成右图的中性灰色,以下描述正确的是:*A.先在图层调板中将“锁定图像像素”按钮选中,再执行“编辑>填充”命令,在弹出的对话框中选择“内容”为 50%灰色,单击“确认”按钮B.执行“图像>调整>色阶”命令,在弹出对话框中,将“输出色阶”数值“0,255”更改为“128,128”,单击“确认”按钮(正确答案)C.执行“图像>调整>曲线”命令,在弹出对话框中,将曲线倾斜的 45 度角调整成水平居中,单击“确认”按钮(正确答案)D.以上答案都对7. 如图所示,图中被选中的文字通过段落面板中的哪些按钮(见上图)可以得到如下图所示的居中效果? *A.AB.B(正确答案)C.C(正确答案)D.D8. 什么色彩模式的图像转换为多通道模式时,建立的通道名称均为Alpha? [单选题] *A.RGB 模式B.CMYK 模式b 模式(正确答案)D.多通道模式9. 如图所示,左上图中的商标部分被快速修复为右下图所示效果,请问可以使用下列哪些工具实现并得到比较自然的溶合修复效果?*1. 修补工具(正确答案)2. 修复画笔工具(正确答案)3. 仿制图章工具4. 图案图章工具10. 在画笔使用中,快速调整画笔硬度的快捷键是: *1. Shift+“[”和Shift+“]”(正确答案)2. “[”和“]”3. Ctrl(Windows 操作系统)/Command(Mac OS X 操作系统)+“[”和 Ctrl (Windows 操作系统)/Command(Mac OS X 操作系统)+“]”4. Ctrl(Windows 操作系统)/Command(Mac OS X 操作系统)+Shift+“[”和 Ctrl (Windows 操作系统)/Command(Mac OS X 操作系统)+Shift+“]”11. 如图所示,左图是使用套索工具创建的选区,要在选区的基础上再添加一个选区如右图所示,下列说法正确的是:*1. 使用套索工具绘制下一个选区,然后按下 Shift 键2. 按住 Shift 键,使用套索工具绘制下一个选区(正确答案)3. 使用套索工具绘制下一个选区,然后按下 Alt 键4. 按住 Alt 键,使用套索工具绘制下一个选区12. 下面对背景色橡皮擦工具与魔术橡皮擦工具描述正确的是: *1.背景色橡皮擦工具与橡皮擦工具使用方法基本相似,背景色橡皮擦工具可将颜色擦掉变成没有颜色的透明部分(正确答案)2.魔术橡皮擦工具可根据颜色近似程度来确定将图像擦成透明的程度(正确答案)3.背景色橡皮擦工具选项栏中的“容差”选项是用来控制擦除颜色的范围(正确答案)4.魔术橡皮擦工具选项栏中的“容差”选项在执行后只擦除图像连续的部分13. 如图所示:上图到下图的变化是通过什么命令得到的: [单选题] *A.色相/饱和度B.替换颜色C.曲线D.渐变映射(正确答案)14. 如图所示,左图的文字通过更改什么段落排版方式后成为右图所示的效果?*连字(正确答案)首行缩进(正确答案)全部对齐最后一行左对齐(正确答案)15. 下列关于快捷批处理的描述正确的是(____)。
Gentamicin竞争性ELISA试验套件目录号:MET-5135 96试验次数说明书
Product ManualGentamicin Competitive ELISA KitCatalog NumberMET-5135 96 assaysFOR RESEARCH USE ONLYNot for use in diagnostic proceduresIntroductionGentamicin is an antibiotic that has been used to treat various types of bacterial infections such as pelvic inflammatory disease, pneumonia, bone infections, endocarditis, meningitis, sepis, and urinary tract infections. Gentamicin was patented in 1962 and approved to be used medically in 1964. Gentamicin can be administered topically, intravenously, or by injection into a muscle. Gentamicin is an aminoglycoside that works by blocking synthesis of bacterial proteins resulting in bacterial death. This antibiotic is made from the bacterium Micromonospora purpurea.As a side effect of treatment, gentamicin can cause inner ear problems (such as hearing loss or poor balance) as well as kidney problems. In addition, gentamicin can cause harm to a developing baby in pregnant women. However, the antibiotic appears to be safe when administered to women who are breastfeeding newborns. Gentamicin is also used by researchers as an antibacterial agent in cell cultures. Gentamicin is a very heat-stable antibiotic, remaining active even after autoclaving, which makes the antibiotic useful in the manufacturing of some types of microbiological growth media.Cell Biolabs’ Gentamicin Competitive ELISA Kit provides a convenient method for the detection of total Gentamicin in extracts from cells, tissue, serum, plasma, urine, or foods. The total content of gentamicin in unknown samples is determined by comparison with a gentamicin standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples. The kit has a detection sensitivity limit of 25 nM gentamicin.Assay PrincipleFirst, a gentamicin conjugate is coated on an ELISA plate. The unknown gentamicin samples or gentamicin standards are then added to the gentamicin conjugate preabsorbed ELISA plate. After a brief incubation, an anti-gentamicin monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The total content of gentamicin in unknown extracted samples is determined by comparison with a gentamicin standard curve.Related Products1.STA-320: OxiSelect™ Oxidative DNA Damage ELISA Kit (8-OHdG Quantitation)2.STA-325: OxiSelect™ Oxidative RNA Damage ELISA Kit (8-OHG Quantitation)3.AKR-110: Rapid GST Inclusion Body Solubilization and Renaturation KitKit ComponentsBox 1 (shipped at room temperature)1.96-well Protein Binding Plate (Part No. 231001): One strip well 96-well plate.2.Anti-Gentamicin Antibody (500X) (Part No. 51351C): One 10 µL vial of anti-GentamicinAntibody.3.Secondary Antibody, HRP Conjugate (1000X) (Part No. 230003): One 20 µL vial.4.Assay Diluent (Part No. 310804):One 50 mL bottle.5.10X Wash Buffer (Part No. 310806): One 100 mL bottle.6.Substrate Solution (Part No. 310807): One 12 mL amber bottle.7.Stop Solution (Part. No. 310808): One 12 mL bottle.Box 2 (shipped on blue ice packs)1.Gentamicin Standard (Part No. 51352C): One 50 µL vial of 150 µM Gentamicin.2.Gentamicin Conjugate (500X) (Part No. 51353C): One 25 µL vial.3.100X Conjugate Diluent (Part No. 281603): One 300 µL vial.Materials Not Supplied1.1X PBS2.10 µL to 1000 µL adjustable single channel micropipettes with disposable tips3.50 µL to 300 µL adjustable multichannel micropipette with disposable tips4.Multichannel micropipette reservoir5.Microplate reader capable of reading at 450 nm (620 nm as optional reference wavelength) StorageUpon receipt, store Anti-Gentamicin Antibody (500X), Gentamicin Standard, Gentamicin Conjugate, and 100X Conjugate Diluent at -20ºC. Store all the remaining components at 4°C.Preparation of Reagents•Gentamicin Conjugate Coated Plate:Note: The Gentamicin Conjugate coated wells are not stable and should be used within 24 hrs after coating. Only coat the number of wells to be used immediately.1.Immediately before use, prepare 1X Conjugate Diluent by diluting the 100X Conjugate Diluentin 1X PBS. Example: Add 50 μL to 4.95 mL of 1X PBS.2.Immediately before use, prepare 1X Gentamicin Conjugate by diluting the 500X GentamicinConjugate in 1X Conjugate Diluent. Example: Add 10 μL of 500X Gentamicin Conjugate to4.99 mL of 1X Conjugate Diluent.3.Add 100 μL of the 1X Gentamicin Conjugate to each well to be tested and incubate overnightat 4ºC. Remove the Gentamicin Conjugate coating solution and wash twice with 1X PBS. Blot plate on paper towels to rem ove excess fluid. Add 200 μL of Assay Diluent to each well and block for 1 hr at room temperature on an orbital shaker. Transfer the plate to 4ºC and remove the Assay Diluent immediately before use.•1X Wash Buffer: Dilute the 10X Wash Buffer to 1X with deionized water. Stir to homogeneity. •Anti-Gentamicin Antibody and Secondary Antibody: Immediately before use, dilute the Anti- Gentamicin antibody 1:500 and Secondary Antibody 1:1000 with Assay Diluent. Do not store diluted solutions.Preparation of Standard CurvePrepare a dilution series of Gentamicin standards in the concentration range of 0 to 1500 nM by diluting the Gentamicin Standard in Assay Diluent (Table 1).Standard Tubes 150 µM GentamicinStandard (µL)Assay Diluent(µL)Gentamicin(nM)1 5 495 15002 200 of Tube #1 200 7503 200 of Tube #2 200 3754 200 of Tube #3 200 1885 200 of Tube #4 200 946 200 of Tube #5 200 477 200 of Tube #6 200 238 0 200 0Table 1. Preparation of Gentamicin StandardsPreparation of Samples•Serum: Avoid hemolyzed and lipemic blood samples. Collect blood in a tube with no anticoagulant. Allow the blood to clot at room temperature for 30 minutes. Centrifuge at 2500 x g for 20 minutes. Remove the yellow serum supernatant without disturbing the white buffy layer.Aliquot samples for testing and store at -80ºC. Perform dilutions in Assay Diluent as necessary. •Plasma: Avoid hemolyzed and lipemic blood samples. Collect blood with heparin or citrate and centrifuge at 2000 x g and 4ºC for 10 minutes. Remove the plasma layer and store on ice. Avoid disturbing the white buffy layer. Aliquot samples for testing and store at -80ºC. Perform dilutions in Assay Diluent as necessary.•Cells or tissues: Homogenize 50-200 mg of the cell pellet or tissue in 0.5-2 mL of ice cold PBS using a mortar and pestle or by dounce homogenization. Incubate the homogenate at 4°C for 20 minutes. Transfer the homogenate to a centrifuge tube and centrifuge at 12000 x g for 20 minutes.Recover the supernatant and transfer to a fresh tube. Store resuspended sample at -20°C or colder.Perform dilutions in Assay Diluent as necessary.•Food samples: Homogenize 1-5 grams in ice cold PBS using a mortar and pestle or by dounce homogenization. Transfer the homogenate to a centrifuge tube and centrifuge at 12000 x g for 20 minutes. Store homogenized sample at -20°C or colder. Perform dilutions in Assay Diluent as necessary.Assay Protocol1.Prepare and mix all reagents thoroughly before use. Each Gentamicin sample including unknownand standard should be assayed in duplicate.2.Add 50 µL of unknown sample or Gentamicin standard to the wells of the Gentamicin Conjugatecoated plate. Incubate at room temperature for 10 minutes on an orbital shaker.3.Add 50 µL of the diluted anti-Gentamicin antibody to each well, incubate at room temperature for 1hour on an orbital shaker.4.Wash 3 times with 250 µL of 1X Wash Buffer with thorough aspiration between each wash. Afterthe last wash, empty wells and tap microwell strips on absorbent pad or paper towel to remove excess 1X Wash Buffer.5.Add 100 µL of the diluted Secondary Antibody-HRP Conjugate to all wells and incubate for 1 hourat room temperature on an orbital shaker. Wash the strip wells 3 times according to step 4 above.6.Warm Substrate Solution to room temperature. Add 100 L of Substrate Solution to each well.Incubate at room temperature for 2-20 minutes on an orbital shaker.Note: Watch plate carefully; if color changes rapidly, the reaction may need to be stopped sooner to prevent saturation.7.Stop the enzyme reaction by adding 100 µL of Stop Solution to each well. Results should be readimmediately (color will fade over time).8.Read absorbance of each well on a microplate reader using 450 nm as the primary wave length.Example of ResultsThe following figures demonstrate typical Gentamicin Competitive ELISA results. One should use the data below for reference only. This data should not be used to interpret actual results.Figure 1: Gentamicin Standard Curve.Figure 2: Specificity of Gentamicin ELISA. G418 (green circles) or Neomycin (red squares) was measured using the Gentamicin Competitive ELISA Kit.References1.Lopez-Novoa JM, Quiros Y, Vicente L, Morales AI, and Lopez-Hernandez FJ (2011). Kidney Int.79: 33–45.2.East JE, Foweraker JE, and Murgatroyd FD (2005). Heart. 91: e32.3.Sato Y (1997). Acta Paediatrica Japonica. 39: 124–131.4.Selimoglu E (2007). Cur. Pharm. Design. 1: 119–126.5.Wilson D (2013). Nature Rev. Microbiol.12: 34–48.Recent Product CitationWijers, C.D.M. et al. (2022). Gram-negative bacteria act as a reservoir for aminoglycoside antibiotics that interact with host factors to enhance bacterial killing in a mouse model of pneumonia. FEMS Microbes. doi: 10.1093/femsmc/xtac016.WarrantyThese products are warranted to perform as described in their labeling and in Cell Biolabs literature when used in accordance with their instructions. THERE ARE NO WARRANTIES THAT EXTEND BEYOND THIS EXPRESSEDWARRANTY AND CELL BIOLABS DISCLAIMS ANY IMPLIED WARRANTY OF MERCHANTABILITY OR WARRANTY OF FITNESS FOR PARTICULAR PURPOSE. CELL BIOLABS’sole obligation and purchaser’s exclusive remedy for breach of this warranty shall be, at the option of CELL BIOLABS, to repair or replace the products. In no event shall CELL BIOLABS be liable for any proximate, incidental or consequential damages in connection with the products.Contact InformationCell Biolabs, Inc.7758 Arjons DriveSan Diego, CA 92126Worldwide: +1 858-271-6500USA Toll-Free: 1-888-CBL-0505E-mail: ********************©2020-2023: Cell Biolabs, Inc. - All rights reserved. No part of these works may be reproduced in any form without permissions in writing.。
- 1、下载文档前请自行甄别文档内容的完整性,平台不提供额外的编辑、内容补充、找答案等附加服务。
- 2、"仅部分预览"的文档,不可在线预览部分如存在完整性等问题,可反馈申请退款(可完整预览的文档不适用该条件!)。
- 3、如文档侵犯您的权益,请联系客服反馈,我们会尽快为您处理(人工客服工作时间:9:00-18:30)。
136. ______ she realized it was too late to go home.
A) It was not until dark that B) No sooner it grew dark than
C) Hardly did it grow dark that D) Scarcely had it grown dark than
私有化的优点在于能够相互竞争.
122. You can not be ______ careful when you drive a car.
A) very B) so C) too D) enough
答案为C 很少见的语法现象 这句话的意思是:开车的时候你不能不小心。
dislike vt. 讨厌, 不喜欢
n. 嫌恶 I felt a strong dislike of the new teacher.
我感到很不喜欢这个新教员。
134. ______ you are leaving tomorrow, we can eat dinner together tonight.
A) was he to make himself hear B) he was able to make himself hear
C) he was able to make himself heard D) was he able to make himself heard
答案为C have sb. do
130. We object ______ punishing a whole group for one person’s fault.
A) against B) about C) to D) for
答案为C object to 反对 to 为介词
141. There are signs ______ restaurants are becoming more popular with families.
答案为B 关于it’s no use 可以说:it’s no use doing, it’s no use sb. doing,
it’s of no use. it’s no use sb’s doing
127. Only by shouting at the top of his voice ______.
A) all the information B) all the informations
C) all of information D) all of the informations
答案为A 首先information是不可数名词 all of 后接代词或带有特指成分的名词
A) to stop B) stop C) stopping D) having stopped
答案为C suggest 为及物动词,后接宾语从句或动名词 没有suggest sb. 这一结构,常说
suggest sth. for sb. to do
too与 can't一起用作否定时,有时会产生歧义,例如在 You can't check your child's temperature too often这句话中,可能意为“你只能偶尔给你孩子量量体温”或“你应该经常给你孩子量体温”。这种用法不能说是错误的,但有些评论家却非常讨厌这种用法。
A) considered B) be considered C) considering D) having considered
答案为A 故去分词的独立结构,表被动
126. It’s no use ______ me not to worry.
A) you tell B) you telling C) for you to have told D) having telling
131. He came back late, ______ which time all the guests had already left.
A) at B) after C) by D) during
答案为C 根据后一句的时态,只能用by.
132. I’ve never been to Beijing, but it’s the place ______.
C) Were other things equal D) Other things to be equal
答案为A 这是一个固定短语。
other things being equal 在其他各点都相同的情况下 (很少见)
125. All things ______, the planned trip will have to be called off.
postpone the match 推迟比赛
delay 指“暂时阻挠或阻挡, 稍后可再继续进行”, 如:
The steamer was delayed by bad weather 汽轮因天气不佳而延期。
138. These books, which you can get at any bookshop, will give you ______ you need.
答案为D 倒装+make sb. done
128. ______ such a good chance, he planned to learn.
A) To be given B) Having been given C) Having given D) Giving
答案为B 分词短语的被动式用于句首作状语,表条件
unlike 还可以作adj. 不相象的;不同的: For twins, they are very unlike. 作为一对双胞胎,他们很不一样
alike adj. 相似的;相同的 The 2 sisters are very alike. 两姐妹非常相像。
adv. 同样地, 相似地 He treats all customers alike. 他对所有的顾客都是一视同仁。
A) that I want to visit it most B) where I’d like to visit
C) in which I’d like to visit D) I most want to visit
答案为D 中心词出现,后接先行词that 或which ,可省略。
129. My sister’s professor had her ______ her paper many times before allowing her to present it to the committee.
A) rewritten B) to rewrite C) rewrite D) rewriting
139. You will see this product ______ wherever you go.
A) to be advertised B) advertised C) advertise D) advertising
答案为B 过去分词做后置定语,表被动
140. Peter, who had been driving all day, suggested ______ at the next town.
答案为D it在本句中指代 the order 因此取消命令只能用cancel
postpone 是正式用语, 语义较强, 指“有意识地延至将来某一特定时间”, 在多数情况下, 后 面说明改在何时进行, 如:
The meeting has been postponed to Friday.会议推迟到星期五举行。
123. e old couple decided to ______ a boy and a girl though they had three children of their own.
A) adapt B) bring C) receive D) adopt
答案为D
133. ______ his sister, Jack is quiet and does not easily make friends with others.
A) Unlike B) Liking C) Alike D) Dislike
答案为A unlike 为介词,不像,不同于
答案为A
not that… for that… 不……是因为…… (罕见)
not that … but that 不是因为……而是因为……
in that=for the reason that 因为,基于……的理由 例句:(前无逗号隔开)
Privatization is thought to be beneficial in that it promotes competition.
A) While B) Since C) For D) Before
答案为B 既然 (作过)
135. Between 1994 and 1997, the number of overseas visitors expanded ______ 27%.
A) in B) for C) by D) to
adopt 采用, 收养 adopt an idea 采纳意见 adopt a report 接受报告 adopt a child 收为养子
adapt 使适应, 改编 He adapted himself to the cold weather. 他适应了寒冷的天气。
The movie was adapted from a novel. 这部电影是由小说改编的。