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Xeomin (incobotulinumtoxinA) 说明书
Xeomin® (incobotulinumtoxinA)Document Number: IC-0241 Last Review Date: 03/29/2016Date of Origin: 06/21/2011Dates Reviewed:09/2011, 12/2011, 03/2012, 06/2012, 09/2012, 12/2012, 02/2013, 03/2013, 06/2013,09/2013, 12/2013, 03/2014, 03/2015, 6/2015, 9/2015, 12/2015, 03/2016I.Length of AuthorizationCoverage is provided for six months and may be renewed.II.Dosing LimitsA.Quantity Limit (max daily dose) [Pharmacy Benefit]:−N/AB.Max Units (per dose and over time) [Medical Benefit]:Xeomin (J0588)Male: 400 billable units per 12 weeks (84 days)Female: 400 billable units per 12 weeks (84 days)III.Initial Approval CriteriaCoverage is provided in the following conditions:Xeomin is considered medically necessary in the following:•Patient age 18 or greater; AND•Cervical dystonia †•Patient has sustained head tilt; OR•Abdominal posturing with limited range of motion in neck; AND•History of recurrent involuntary contraction of one or more muscles in the neckBlepharospasms †Upper limb spasticity ††FDA Approved Indication(s)IV.Renewal CriteriaCoverage can be renewed based upon the following criteria:•Patient continues to meet criteria identified in section III; AND•Disease response; AND•Absence of unacceptable toxicity from the drugV.Dosage/AdministrationCervical Dystonia 120 units divided among the affected muscles every 12 weeks orlonger, as necessaryBlepharospasm 1.25 – 5.6 units per injection site, not to exceed 35 units per eye, every12 weeks or longer, as necessaryUpper limb spasticity Up to 400 units total no sooner than every 12 weeksVI.Billing Code/Availability InformationJcode:•J0588 – Xeomin (Merz GmbH) 50 units, 100 units Injection: 1 billable unit = 1 unitNDC:N/AVII.References1.Xeomin [package insert]. Dessau-Rosslau, Germany; Merz Group Services GmbH;December 2015. Accessed January 2015.2.Simpson DM, Blitzer A, Brashear A, Comella C, Dubinsky R, Hallett M, Jankovic J, KarpB, Ludlow CL, Miyasaki JM, Naumann M, So Y, Therapeutics and Technology AssessmentSubcommittee of the American Academy of Neurology. Assessment: Botulinum neurotoxinfor the treatment of movement disorders (an evidence-based review): report of theTherapeutics and Technology Assessment Subcommittee of the American Academy ofNeurology. Neurology 2008 May 6;70(19):1699-706.3.Cahaba Government Benefit Administrators, LLC. Local Coverage Determination (LCD):Botulinum Toxins (L34253). Centers for Medicare & Medicaid Services, Inc. Updated on12/16/2015 with effective date 10/1/2015. Accessed January 2016.4.CGS, Administrators, LLC. Local Coverage Determination (LCD): Botulinum Toxins(L33949). Centers for Medicare & Medicaid Services, Inc. Updated on 11/23/2015 witheffective date 10/1/2015. Accessed January 2016.5.First Coast Service Options, Inc. Local Coverage Determination (LCD): Botulinum Toxins(L33274). Centers for Medicare & Medicaid Services, Inc. Updated on 07/01/2014 witheffective date 10/1/2015. Accessed January 2016.6.National Government Services, Inc. Local Coverage Determination (LCD): BotulinumToxins (L33646). Centers for Medicare & Medicaid Services, Inc. Updated on 10/30/2015 with effective date 10/1/2015. Accessed January 2016.7.Noridian Administrative Services, LLC. Local Coverage Determination (LCD): BotulinumToxin Types A and B (L35172). Centers for Medicare & Medicaid Services, Inc. Updated on 11/20/2015 with effective date 10/1/2015. Accessed January 2016.8.Noridian Healthcare Solutions, LLC. Local Coverage Determination (LCD): BotulinumToxin Types A and B (L35170). Centers for Medicare & Medicaid Services, Inc. Updated on 11/20/2015 with effective date 10/1/2015. Accessed January 2016.9.Palmetto GBA. Local Coverage Determination (LCD): Chemodenervation (L33458).Centers for Medicare & Medicaid Services, Inc. Updated on 11/20/2015 with effective date 12/16/2015. Accessed January 2016.10.Wisconsin Physicians Service Insurance Corporation. Local Coverage Determination(LCD): Botulinum Toxin Type A & Type B (L34635). Centers for Medicare & MedicaidServices, Inc. Updated on 11/16/2015 with effective date 12/1/2015. Accessed January2016.Appendix 1 – Covered Diagnosis Codes333.81 Blepharospasm333.83 Spasmodic torticollis342.10 Spastic hemiplegia and hemiparesis affecting unspecified side342.11 Spastic hemiplegia and hemiparesis affecting dominant side342.12 Spastic hemiplegia and hemiparesis affecting nondominant side343.0 Diplegic; Congenital diplegia; Congenital paraplegia343.1 Hemiplegic; Congenital hemiplegia343.2 Quadriplegic; Tetraplegic344.03 Quadriplegia, C5-C7, complete344.04 Quadriplegia, C5-C7, incomplete344.2 Diplegia of upper limbs344.40 Monoplegia of upper limb affecting unspecified side344.41 Monoplegia of upper limb affecting dominant side344.42 Monoplegia of upper limb affecting nondominant side438.20 Late effects of cerebrovascular disease, hemiplegia affecting unspecified side438.21 Late effects of cerebrovascular disease, hemiplegia affecting dominant side438.22 Late effects of cerebrovascular disease, hemiplegia affecting non-dominant side438.30 Late effects of cerebrovascular disease; monoplegia of upper limb affecting unspecified side 438.31 Late effects of cerebrovascular disease; monoplegia of upper limb affecting dominant side 438.32 Late effects of cerebrovascular disease; monoplegia of upper limb affecting nondominantside723.5 Torticollis, unspecifiedG24.3 Spasmodic torticollisG24.5 BlepharospasmG80.1 Spastic diplegic cerebral palsyG80.2 Spastic hemiplegic cerebral palsyG80.3 Spastic quadriplegic cerebral palsyG81.10 Spastic hemiplegia affecting unspecified sideG81.11 Spastic hemiplegia affecting right dominant sideG81.12 Spastic hemiplegia affecting left dominant sideG81.13 Spastic hemiplegia affecting right nondominant sideG81.14 Spastic hemiplegia affecting left nondominant sideG82.53 Quadriplegia, C5-C7, completeG82.54 Quadriplegia, C5-C7, incompleteG83.0 Diplegia of upper limbs, Diplegia (Upper), Paralysis of both upper limbs G83.20 Monoplegia of upper limb affecting unspecified sideG83.21 Monoplegia of upper limb affecting right dominant sideG83.22 Monoplegia of upper limb affecting left dominant sideG83.23 Monoplegia of upper limb affecting right nondominant sideG83.24 Monoplegia of upper limb affecting left nondominant sideI69.031 Monoplegia of upper limb following nontraumatic subarachnoid hemorrhage affecting right dominant sideI69.032 Monoplegia of upper limb following nontraumatic subarachnoid hemorrhage affecting left dominant sideI69.033 Monoplegia of upper limb following nontraumatic subarachnoid hemorrhage affecting right non-dominant sideI69.034 Monoplegia of upper limb following nontraumatic subarachnoid hemorrhage affecting left non-dominant sideI69.039 Monoplegia of upper limb following nontraumatic subarachnoid hemorrhage affecting unspecified sideI69.051 Hemiplegia and hemiparesis following nontraumatic subarachnoid hemorrhage affecting right dominant sideI69.052 Hemiplegia and hemiparesis following nontraumatic subarachnoid hemorrhage affecting left dominant sideI69.053 Hemiplegia and hemiparesis following nontraumatic subarachnoid hemorrhage affecting right non-dominant sideI69.054 Hemiplegia and hemiparesis following nontraumatic subarachnoid hemorrhage affecting left non-dominant sideI69.059 Hemiplegia and hemiparesis following nontraumatic subarachnoid hemorrhage affecting unspecified sideI69.131 Monoplegia of upper limb following nontraumatic intracerebral hemorrhage affecting right dominant sideI69.132 Monoplegia of upper limb following nontraumatic intracerebral hemorrhage affecting left dominant sideI69.133 Monoplegia of upper limb following nontraumatic intracerebral hemorrhage affecting right non-dominant sideI69.134 Monoplegia of upper limb following nontraumatic intracerebral hemorrhage affecting left non-dominant sideI69.139 Monoplegia of upper limb following nontraumatic intracerebral hemorrhage affecting unspecified siteI69.151 Hemiplegia and hemiparesis following nontraumatic intracerebral hemorrhage affecting right dominant sideI69.152 Hemiplegia and hemiparesis following nontraumatic intracerebral hemorrhage affecting left dominant sideI69.153 Hemiplegia and hemiparesis following nontraumatic intracerebral hemorrhage affecting right non-dominant sideI69.154 Hemiplegia and hemiparesis following nontraumatic intracerebral hemorrhage affecting left non-dominant sideI69.159 Hemiplegia and hemiparesis following nontraumatic intracerebral hemorrhage affecting unspecified sideI69.231 Monoplegia of upper limb following other nontraumatic intracranial hemorrhage affecting right dominant sideI69.232 Monoplegia of upper limb following other nontraumatic intracranial hemorrhage affecting left dominant sideI69.233 Monoplegia of upper limb following other nontraumatic intracranial hemorrhage affecting right non-dominant sideI69.234 Monoplegia of upper limb following other nontraumatic intracranial hemorrhage affecting left non-dominant sideI69.239 Monoplegia of upper limb following other nontraumatic intracranial hemorrhage affecting unspecified siteI69.251 Hemiplegia and hemiparesis following other nontraumatic intracranial hemorrhageaffecting right dominant sideI69.252 Hemiplegia and hemiparesis following other nontraumatic intracranial hemorrhage affecting left dominant sideI69.253 Hemiplegia and hemiparesis following other nontraumatic intracranial hemorrhage affecting right non-dominant sideI69.254 Hemiplegia and hemiparesis following other nontraumatic intracranial hemorrhage affecting left non-dominant sideI69.259 Hemiplegia and hemiparesis following other nontraumatic intracranial hemorrhage affecting unspecified sideI69.331 Monoplegia of upper limb following cerebral infarction affecting right dominant sideI69.332 Monoplegia of upper limb following cerebral infarction affecting left dominant sideI69.333 Monoplegia of upper limb following cerebral infarction affecting right non-dominant side I69.334 Monoplegia of upper limb following cerebral infarction affecting left non-dominant side I69.339 Monoplegia of upper limb following cerebral infarction affecting unspecified siteI69.351 Hemiplegia and hemiparesis following cerebral infarction affecting right dominant side I69.352 Hemiplegia and hemiparesis following cerebral infarction affecting left dominant sideI69.353 Hemiplegia and hemiparesis following cerebral infarction affecting right non-dominant sideI69.354 Hemiplegia and hemiparesis following cerebral infarction affecting left non-dominant sideI69.359 Hemiplegia and hemiparesis following cerebral infarction affecting unspecified sideI69.831 Monoplegia of upper limb following other cerebrovascular disease affecting right dominant sideI69.832 Monoplegia of upper limb following other cerebrovascular disease affecting left dominant sideI69.833 Monoplegia of upper limb following other cerebrovascular disease affecting right non-dominant sideI69.834 Monoplegia of upper limb following other cerebrovascular disease affecting left non-dominant sideI69.839 Monoplegia of upper limb following other cerebrovascular disease affecting unspecified siteI69.851 Hemiplegia and hemiparesis following other cerebrovascular disease affecting right dominant sideI69.852 Hemiplegia and hemiparesis following other cerebrovascular disease affecting left dominant sideI69.853 Hemiplegia and hemiparesis following other cerebrovascular disease affecting right non-dominant sideI69.854 Hemiplegia and hemiparesis following other cerebrovascular disease affecting left non-dominant sideI69.859 Hemiplegia and hemiparesis following other cerebrovascular disease affecting unspecified sideI69.931 Monoplegia of upper limb following unspecified cerebrovascular disease affecting right dominant sideI69.932 Monoplegia of upper limb following unspecified cerebrovascular disease affecting left dominant sideI69.933 Monoplegia of upper limb following unspecified cerebrovascular disease affecting right non-dominant sideI69.934 Monoplegia of upper limb following unspecified cerebrovascular disease affecting left non-dominant sideI69.939 Monoplegia of upper limb following unspecified cerebrovascular disease affecting unspecified sideI69.951 Hemiplegia and hemiparesis following unspecified cerebrovascular disease affecting right dominant sideI69.952 Hemiplegia and hemiparesis following unspecified cerebrovascular disease affecting left dominant sideI69.953 Hemiplegia and hemiparesis following unspecified cerebrovascular disease affecting right non-dominant sideI69.954 Hemiplegia and hemiparesis following unspecified cerebrovascular disease affecting left non-dominant sideI69.959 Hemiplegia and hemiparesis following unspecified cerebrovascular disease affecting unspecified sideM43.6 TorticollisAppendix 2 – Centers for Medicare and Medicaid Services (CMS)Medicare coverage for outpatient (Part B) drugs is outlined in the Medicare Benefit Policy Manual (Pub. 100-2), Chapter 15, §50 Drugs and Biologicals. In addition, National Coverage Determination (NCD) and Local Coverage Determinations (LCDs) may exist and compliance with these policies is required where applicable. They can be found at: /medicare-coverage-database/search/advanced-search.aspx. Additional indications may be covered at the discretion of the health plan.Medicare Part B Covered Diagnosis Codes (applicable to existing NCD/LCD):Jurisdiction(s): 10 (J) NCD/LCD Document (s): L34253https:///medicare-coverage-database/details/lcd-details.aspx?LCDId=34253&ver=12&Date=02%2f01%2f2016&DocID=L34253&SearchType=Adva nced&bc=KAAAAAgAAAAAAA%3d%3d&Jurisdiction(s): 15 NCD/LCD Document (s): L33949https:///medicare-coverage-database/details/lcd-details.aspx?LCDId=33949&ver=7&Date=02%2f01%2f2016&DocID=L33949&SearchType=Advan ced&bc=KAAAAAgAAAAAAA%3d%3d&Jurisdiction(s): 9 (N) NCD/LCD Document (s): L33274https:///medicare-coverage-database/details/lcd-details.aspx?LCDId=33274&ver=3&Date=02%2f01%2f2016&DocID=L33274&SearchType=Advan ced&bc=KAAAAAgAAAAAAA%3d%3d&Jurisdiction(s): 11 (M) NCD/LCD Document (s): L33458https:///medicare-coverage-database/details/lcd-details.aspx?LCDId=33458&ver=20&Date=02%2f01%2f2016&DocID=L33458&SearchType=Adva nced&bc=KAAAAAgAAAAAAA%3d%3d&Jurisdiction(s): 6,K NCD/LCD Document (s): L33646https:///medicare-coverage-database/details/lcd-details.aspx?LCDId=33646&ver=11&Date=02%2f01%2f2016&DocID=L33646&SearchType=Adva nced&bc=KAAAAAgAAAAAAA%3d%3d&Jurisdiction(s): F NCD/LCD Document (s): L35172https:///medicare-coverage-database/details/lcd-details.aspx?LCDId=35172&ver=19&Date=02%2f01%2f2016&DocID=L35172&SearchType=Adva nced&bc=KAAAAAgAAAAAAA%3d%3d&Jurisdiction(s): (J-E) NCD/LCD Document (s): L35170https:///medicare-coverage-database/details/lcd-details.aspx?LCDId=35170&ver=11&Date=02%2f01%2f2016&DocID=L35170&SearchType=Adva nced&bc=KAAAAAgAAAAAAA%3d%3d&Jurisdiction(s): 5, 8 NCD/LCD Document (s): L34635https:///medicare-coverage-database/details/lcd-details.aspx?LCDId=34635&ver=12&Date=02%2f01%2f2016&DocID=L34635&SearchType=Adva nced&bc=KAAAAAgAAAAAAA%3d%3d&Jurisdiction Applicable State/US Territory ContractorE CA,HI, NV, AS, GU, CNMI Noridian Administrative Services (NAS)F AK, WA, OR, ID, ND, SD, MT, WY,Noridian Administrative Services (NAS) UT, AZ5 KS, NE, IA, MO Wisconsin Physicians Service (WPS)6 MN, WI, IL National Government Services (NGS)H LA, AR, MS, TX, OK, CO, NM Novitas Solutions8 MI, IN Wisconsin Physicians Service (WPS)9 (N) FL, PR, VI First Coast Service Options10 (J) TN, GA, AL Cahaba Government Benefit Administrators11 (M) NC, SC, VA, WV Palmetto GBA12 (L) DE, MD, PA, NJ, DC Novitas SolutionsK NY, CT, MA, RI, VT, ME, NH National Government Services (NGS)15 KY, OH CGS Administrators, LLC。
mysql索引类型normal,unique,fulltext
mysql索引类型normal,unique,fulltext问题1:mysql索引类型normal,unique,full text的区别是什么?normal:表⽰普通索引unique:表⽰唯⼀的,不允许重复的索引,如果该字段信息保证不会重复例如⾝份证号⽤作索引时,可设置为uniquefull textl: 表⽰全⽂搜索的索引。
FULLTEXT ⽤于搜索很长⼀篇⽂章的时候,效果最好。
⽤在⽐较短的⽂本,如果就⼀两⾏字的,普通的INDEX 也可以。
总结,索引的类别由建⽴索引的字段内容特性来决定,通常normal最常见。
问题2:在实际操作过程中,应该选取表中哪些字段作为索引?为了使索引的使⽤效率更⾼,在创建索引时,必须考虑在哪些字段上创建索引和创建什么类型的索引,有7⼤原则:1.选择唯⼀性索引2.为经常需要排序、分组和联合操作的字段建⽴索引3.为常作为查询条件的字段建⽴索引4.限制索引的数⽬5.尽量使⽤数据量少的索引6.尽量使⽤前缀来索引7.删除不再使⽤或者很少使⽤的索引⼀、 MySQL: 索引以B树格式保存 Memory存储引擎可以选择Hash或BTree索引,Hash索引只能⽤于=或<=>的等式⽐较。
1、普通索引:create index on Tablename(列的列表) alter table TableName add index (列的列表) create table TableName([...], index [IndexName] (列的列表) 2、唯⼀性索引:create unique index alter ... add unique 主键:⼀种唯⼀性索引,必须指定为primary key 3、全⽂索引:从3.23.23版开始⽀持全⽂索引和全⽂检索,FULLTEXT, 可以在char、varchar或text类型的列上创建。
4、单列索引、多列索引: 多个单列索引与单个多列索引的查询效果不同,因为: 执⾏查询时,MySQL只能使⽤⼀个索引,会从多个索引中选择⼀个限制最为严格的索引。
文献检索与利用 试题题库及答案
P.S:标记含义____ 基本肯定是答案——不肯定是正确答案**** 一些辅助注释等.《文献检索与利用》总复习题库一、单项选择题1. 以下不是布尔逻辑算符的是()A.NOTB.ORC.ANDD.NEAR2.布尔逻辑算符通常的运算顺序是():A.有括号时,括号内的先执行;无括号时 NOT > AND > OR B.有括号时,括号内的先执行;无括号时 NOT > OR >AND C.有括号时,括号内的先执行;无括号时 AND >NOT > OR D.有括号时,括号内的先执行;无括号时 AND > OR > NOT3.截词符“?”可以用来代替0个或()个字符?A.多个B.1个C.2个D.3个4.以下哪个是图书馆公共目录检索系统的简称?A. CalisB. NSTLC. OCLCD. OPAC5.ISSN号是哪种文献特有的标识?A.会议文献B.标准文献C.学位论文D.期刊6.ISBN号是哪种文献特有的标识?A.图书B.期刊C.科技报告D.专利文献7.下列哪个数据库是全文数据库A.CPCIB.Elsevier Science DirectC.EID. SCI8.用Adobe Reader可以阅读以下哪种格式的文件A .PDFB. VIPC. HTMLD. TXT9.cajviewer是下面哪个数据库全文的阅读软件:A.超星数字图书馆B.维普中文科技期刊全文数据库KI中国知网期刊全文库D.万方数据资源10.浏览超星数字图书馆,应首先安装:A. Apabi ReaderB. Adobe ReaderC. CAJ ViewerD. SSReader11.下列数据库属于书目数据库的是A. SCIB. ISTPC.EID.图书馆OPAC12.PQDT是A.会议文献数据库B.学位论文数据库C.标准文献数据库D.科技报告数据库13.AD、PB、NASA、DOE 是四大美国政府报告,其中NASA是指A.行政报告B.能源报告C.军事报告D.宇航报告14.()是系统反映人类一切知识门类或某一知识门类基本知识和基本情况的大型资料性、综合性工具书,被称为“工具书之王”。
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ORIGINAL PAPEROptimization of parameters for semiempirical methods V: Modification of NDDO approximations and application to70elementsJames J.P.StewartReceived:9May2007/Accepted:10July2007/Published online:9September2007#Springer-Verlag2007Abstract Several modifications that have been made to the NDDOcore-coreinteractiontermandtothemethodofparameter optimization are described.These changes have resulted in a more complete parameter optimization,called PM6,which has, in turn,allowed70elements to be parameterized.The average unsigned error(AUE)between calculated and reference heats of formationfor4,492specieswas8.0kcalmol−1.For the subset of 1,373compounds involving only the elements H,C,N,O,F, P,S,Cl,and Br,the PM6AUE was4.4kcal mol−1.The equivalent AUE for other methods were:RM1:5.0,B3LYP 6–31G*:5.2,PM5:5.7,PM3:6.3,HF6–31G*:7.4,and AM1:10.0kcal mol−1.Several long-standing faults in AM1 and PM3have been corrected and significant improvements have been made in the prediction of geometries. Keywords NDDO.Parameterization.PM6.Transition metalsIntroductionOver the past30years,NDDO-type[1,2]semiempirical methods have evolved steadily.The earliest of these methods was MNDO[3,4],which itself was a major advance over even earlier non-NDDO methods such as MINDO/3[5].The main advantage of MNDO over earlier methods was that the values of the parameters were optimized to reproduce molecular rather than atomic properties.When it first appeared,MNDO was immediately popular because of its increased accuracy,but,with the passage of time,various limitations were found,among the most important of which was the almost total absence of a hydrogen bond.As hydrogen bonding is essential to life, this particular fault essentially precluded MNDO being used in modeling biochemistry.In1985an attempt,AM1[6],was made to improve MNDO by adding a stabilizing Gaussian function to the core-core interaction to represent the hydrogen bond. Despite the fact that this was an over-simplification of a very complicated phenomenon,the overall effect was similar,and for the first time NDDO methods gave a good, albeit limited,model of hydrogen bonding.In the course of the next several years,improvements were made to the method of parameter optimization.The result of this was the PM3method[7–10],which culminated in the parameterization of all the elements in the main group in2004[11].At the same time,various changes to the original set of approximations used in MNDO were proposed,the most important of which were the addition of d-orbitals to main-group elements[12,13] and the introduction of diatomic parameters.Work started on the transition metals,and parameters for some of these have been reported[14,15].More recently,parameter sets tailored to reproduce specific phenomena such as the binding energy of nucleic acid base pairs[16],iron complex catalyzed hydrogen abstraction[17],phosphatase-catalyzed reaction barriers[18],and the redox properties of iron containing proteins[19]have been developed.Because of the way advances in NDDO developments occurred,in terms of the modifications of the approximationsJ Mol Model(2007)13:1173–1213DOI10.1007/s00894-007-0233-4Electronic supplementary material The online version of this article (doi:10.1007/s00894-007-0233-4)contains supplementary material, which is available to authorized users.J.J.P.Stewart(*)Stewart Computational Chemistry,15210Paddington Circle,Colorado Springs,CO80921,USAe-mail:MrMOPAC@and the extensions to specific elements or groups of elements, there has been an inevitable lack of consistency.The aim of the current work was three-fold:to investigate the incorporation of some of the reported modifications to the core-core approxima-tions into the NDDO methodology;to carry out a systematic global parameter optimization of all the main group elements, with emphasis on compounds of interest in biochemistry;and to extend the methodology by performing a restricted optimization of parameters for the transition metals.This resulted in the development of a new method,consisting of the final set of approximations used and the optimized parameters.This method will be referred to as parametric method number6,or PM6.The name PM6was chosen to avoid any confusion with two other unpublished methods,PM4and PM5.TheoryDespite the apparent complexity of semiempirical methods, there are only three possible sources of error:reference data may be inaccurate or inadequate,the set of approximations may include unrealistic assumptions or be too inflexible, and the parameter optimization process may be incomplete. In order for a method to be accurate,all three potential sources of error must be carefully examined,and,where faults are found,appropriate corrective action taken.Reference dataIn contrast to earlier methods,in which reference data was assembled by painstakingly searching the original literature, the current work relies heavily on the large compendia of data that have been developed in recent years.The most important of these are the WebBook[20],for thermochem-istry,and the Cambridge Structural Database[21](CSD), for molecular geometries.During the early stages of the current work,consistency checks were performed to ensure that erroneous data were not used.These checks revealed many cases in which the calculated heats of formation were inconsistent with the reference heats of formation reported in the NIST database. On further checking,many of these reference data were also found[22,23]to be inconsistent with other data in the WebBook.In those cases where there was strong evidence of error in the reference data,the offending data were deleted,and the webbook updated[24].For molecular geometries,gas phase reference data are preferred,but in many instances such data were unavail-able,and recourse was made to condensed-phase data. Provided that care was taken to exclude those species whose geometries were likely to be significantly distorted by crystal forces,or which carried a large formal charge, condensed-phase data of the type found in the CSD were regarded as being suitable as reference data.Because earlier methods used only a limited number of reference data,most of the cases where the method gave bad results were not discovered until after the method was published.In an attempt to minimize the occurrence of such unpleasant surprises,the set of reference data used was made as large as practical.To this end,where there was a dearth or even a complete absence of experimental reference data, recourse was made to high level calculations.Thus,for the Group VIII elements,there are relatively few stable com-pounds,and the main phenomena of interest involve rare gas atoms colliding with other atoms or molecules,so reference data representing the mechanics of rare gas atoms colliding with other atoms was generated from the results of ab-initio calculations.Additionally,there is an almost complete lack of thermochemical data for many types of complexes involving transition metals,so augmenting what little data there was with the results of ab-initio calculations was essential.Use of Ab-Initio resultsAb-initio calculations provide a convenient source of reference data;for this work,extensive use has been made of results of Hartree Fock and B3LYP density functional[25,26]methods (DFT),both with the6–31G(d)basis set for elements in the periodic table up to argon.For systems involving heavier elements,the B88–PW91functional[27,28]was used with the DZVP basis set.Within the spectrum of ab-initio methods these methods are not particularly accurate;many methods with larger basis sets and with post-Hartree-Fock corrections are more accurate.However,the methods used in this work were chosen because they were regarded as robust,practical methods,allowing many systems to be modeled in a reasonable amount of time,a condition that could not be achieved with the more sophisticated ab-initio methods. Procedure used in derivingΔH fReference heats of formation,ΔH f,for compounds and ions of elements for which there was a paucity of data were derived from DFT total energies in two stages.In the first stage,a basic set of∼1,400well-behaved compounds,for which reliable reference values of experimentalΔH f were available, was assembled.Only compounds containing one or more of the elements H,C,N,O,F,P,S,Cl,Br,and I were used.For this set,a root-mean-square fit was made to the referenceΔH f using the calculated total energies,E tot and the atom counts. Thus,the error function,S,in Eq.(1)was minimized.S¼XjΔH j Re f:ðÞÀ627:51E TotþXiC i n i!!2jð1ÞIn this expression,the C i are constants for each atom of type i, and the n i are the number of atoms of that type.In the second stage,the contribution to the total energy of compounds containing element X arising from the elements in the first stage was removed using the coefficients from Equation(1).A second RMS fit was then performed.In this,the function minimized,S,was the RMS difference between the referenceΔH f of compound X and the values predicted from the DFT energy,Eq.(2).S¼Xj ΔH j Ref:ðÞÀ627:51E TotþXiC i n iþC x n x!!2jð2ÞIn this expression,the only unknown is the multiplier coefficient C x.After solving for C x,theΔH f of any compound of X could then be predicted as soon as its DFT total energy was evaluated.Training set reference dataThe training set of reference data used was considerably larger than that used in parameterizing PM3[7,8],where approximately800discrete species were used.In optimiz-ing the parameters for PM6,somewhat over9,000separate species were used,of which about7,500were well-behaved stable molecules.The remainder consisted of reference data that were tailored to help define the values of individual parameters or sets of parameters.Use of rules in parameter optimizationMost reference data can be expressed as simple facts.Indeed, all the earlier NDDO methods were parameterized using precisely four types of reference data:ΔH f,molecular geometries,dipole moments,and ionization potentials.During the development of PM6,however,the use of other types of reference data was found to be necessary.Because of their behavior,these new data are best described as“rules.”In this context,a rule can therefore be regarded as a reference datum that is a function of one or more other data.To illustrate the use of a rule,consider the binding energy of a hydrogen bond in the water dimer.By default,the weighting factor forΔH f for normal compounds is1.0kcal mol−1.With this weighting factor,average unsigned errors in the predictedΔH f of the order of3–5kcal mol−1would be acceptable,particularly as the spectrum of values ofΔH f spans several hundreds of kilocalories per mole.However,the binding energy of a hydrogen bond in a water dimer is only5kcal mol−1.To have an average unsigned error(AUE)of4kcal mol−1in the prediction of hydrogen bond energies would render such a method almost useless for modeling such phenomena.One way to increase the importance of the hydrogen bond in water would be to increase the weight for theΔH f of the water molecule,−57.8kcal mol−1,and the water dimer system,ca.−120.6kcal mol−1.While this would have the intended effect of increasing the weight of the hydrogen bond energy,it would also have the undesired effect of increasing the weight of theΔH f of water.An alternative would be to express theΔH f of the water dimer in terms of theΔH f of two individual water molecules. The difference between the twoΔH f,that of water dimer and that of two isolated water molecules,would be the energy of the hydrogen bond.If the weight assigned to this quantity were then increased,it would increase the weight for the hydrogen bond energy without also increasing the weight for theΔH f of water.Such a reference datum is referred to here as a rule.That is,rules relate theΔH f of a moiety to that of one or more other moieties.Thus,in the above example,the simple reference datum H,representing theΔH f of an isolated water molecule,could be expressed as:H¼À57:8Using a rule-based reference datum to represent the strength of the hydrogen bond,and giving a weight of10to the hydrogen bond energy,theΔH f of the water dimer would then be defined asH¼10À5þH H2OþH H2OðÞIn this expression,H H2O was the calculatedΔH f,in kcal mol−1,of an isolated water molecule.This rule could be interpreted as“The calculated strength of the hydrogen bond formed when two water molecules form the dimer should be5kcal mol−1,and the importance should be100 times that of ordinary heats of formation.”Rules are very useful in defining the parameter hyper-surface.Examples of such tailoring are as follows: Correcting qualitatively incorrect predictionsDuring the parameterization of transition metals,some systems were predicted to have qualitatively the wrong structure.For example,[Cu II Cl4]2−was initially predicted to have a tetrahedral structure,instead of the D2d geometry observed. To induce the parameters to change so as to make the D2d geometry more stable than the T d geometry,a rule was added to the set of reference data for copper compounds.This rule was constructed using the results of B3LYP calculations on [Cu II Cl4]2−.First,the total energies of the optimized B3LYP structure and that of the structure resulting from the semiempirical calculation were evaluated.The difference between these energies was then used in constructing the rule.In this case,the rule was that“TheΔH f of the geometry predicted by the faulty semiempirical method should be n.n kcal mol−1more than that of the B3LYP geometry.”When such a rule was included in the parameter optimization,with an appropriate large weight,any tendency of the parameters to predict the incorrect geometry resulted in a large contributionto the error function.That is,with the new rule in place,there was a strong disincentive to prediction of the incorrect ually one rule was sufficient to correct most qualitative errors,but for a few complicated structures more than one rule was needed.The commonest need for multiple rules occurred when,initially,one rule was used to correct a faulty prediction and,after re-optimizing the parameters,the geometry optimized to a new structure that was distinctly different from either the correct structure or the incorrect structure covered by the rule.When that happened,the procedure just described was repeated,and a new rule added to the set of reference data to address the new incorrect structure.In extreme cases,several such rules might be needed,each one defining a geometry that was incorrect and should therefore be avoided.Rare gas atoms at sub-equilibrium distancesFor some elements,specifically those of Group VIII,there is an understandable shortage of useful experimental reference data. In addition,most simulations involving these elements are likely to involve a rare-gas atom dynamically interacting with another atom or with a molecule at distances significantly less than the equilibrium distance.This makes determining the potential energy surface at sub-equilibrium distances important. As with hydrogen bond energies,the energies involved in this domain are likely to be in the order of a few kcal mol−1.The shape of the potential energy surface(PES)can readily be mapped using DFT methods.By selecting two or three representative points on this PES,reference data rules can be constructed that describe the mechanical properties of the interactions.As with hydrogen bonding,a large weight can be assigned to these rules.Use of rules to restrain parameter valuesIn general,uncharged atoms that are separated by a distance sufficiently large so that all overlaps between orbitals on the two atoms are vanishingly small will not interact significantly, and what interaction energy exists would arise from VDW terms:of their nature,these are mildly stabilizing.Although statements of this type are obviously true,when they are expressed as rules and added to the training set of reference data they can help define the parameter values.For a pair of atoms, A and B,a simple diatomic system would be constructed in which the interatomic separation was the minimum distance at which any overlaps of the atomic orbitals would still be insignificant.The electronic state of such a system would then be the sum of the states of the two isolated atoms.Thus,if both A and B were silicon,then,since the ground state of an isolated silicon atom is a triplet,the combined state would be a quintet. Because the two atoms do not interact significantly,a rule could then be constructed that said“The energy of the diatomic system is equal to the addition of energies of the two individual systems.”By giving this rule a large weight,any tendency of the method to generate a spurious attraction or repulsion between the atoms would be prevented.Atomic energy levelsIn keeping with the philosophy that a large amount of reference data should be used in the parameter optimization,spin-free atomic energy levels were used for most elements.The exceptions were carbon,nitrogen,and oxygen,where there were enough conventional reference data that the addition of atomic energy levels would not significantly improve the definition of the parameter surface.NDDO approximations do not allow for spin-orbit coupling.Therefore,spin-free levels were needed.For a few elements,there were insufficient spin states to allow the spin-free energy levels to be calculated.For all the remaining elements,spin-free energy levels were calculated.In Moore’s compendia[29–31]of atomic energy levels, observed emission spectra were used in determining the energy levels of the various states of neutral and ionized atoms.Most of these energy levels were characterized by three quantum numbers:the spin and orbital angular momenta,and the“J”or spin-orbit quantum number.The starting point for determining the spin-free atomic energy levels for a given element consisted of identifying each complete manifold of atomic energy levels for that element,that is,each set of levels split by spin-orbit coupling.If all members of the set were present,i.e.,all energy levels from L+S to|L−S|,then the weighted barycenter of energy could be calculated.The spin-free energy level,E,was derived from the spin-split levels E (S,L,J)using Eq.(3).E¼12Sþ1ðÞ2Lþ1ðÞXLþSJ¼LÀSj j2Jþ1ðÞE S;L;JðÞð3ÞIn those cases where the ground state of an atom was itself a member of a spin-split manifold,the barycenter of the ground state manifold was calculated and used in re-defining the spin-free ground state.For all elements except tungsten,this change in definition was benign.There is a7S3level present in tungsten that is located only8.4kcal mol−1above the ground state.This puts it inside the5D J,manifold,which has a barycenter at 12.7kcal mol−1.The effect of this was that,on going from a spin-split to a spin-free ground state,the ground state changed from6d25d4or5D to6d15d5or7S,and the5D state now became an excited state with an energy of4.4kcal mol−1.To allow for this,a corresponding change was made to the ground state configuration in the PM6definition of tungsten.Where there were relatively few other reference data,the singly-ionized,and,in rare cases,the doubly-ionized,spin-free states were also evaluated and used as reference data.Each energy level contributed one reference datum to the training set.Most atoms have a large number of atomic energy levels,so in order to minimize the probability that a level might be incorrectly assigned,each level was labeled with three quantum numbers:the total spin momentum,the total angular momentum,and the principal quantum number for these two quantum numbers.These were compared with the corresponding values calculated from the state functions.Since each set of three quantum numbers is unique,the potential for miss-assignment was minimized.In rare cases,particularly during the early stages of parameter optimization,two states with the same total spin and angular quantum numbers would be interchanged,with the result that the calculated principal quantum number would also be interchanged.All such cases always involved the ground state,and were quickly identified and corrected.ApproximationsMost of the approximations used in PM6are identical to those in AM1and PM3.The differences are:Core-core interactionsIn the original MNDO set of approximations,two changes were made to the simple point-charge expression for the core-core repulsion term.Beyond about five Ångstroms,there should be no significant interaction of two neutral atoms.However,in MNDO,the two-electron,two-center s A s A j s B s B i h integrals and the electron-core interactions do not converge to the exact point charge expression;instead,they are always slightly smaller.To prevent there being a small net repulsion between two uncharged atoms,the core-core expression is modified by the exact 1/R AB term being replaced by the term used in the s A s A j s B s B i h integrals.An additional term is needed to represent the increased core-core repulsion at small distances due to the unpolarizable core.These two changes can be expressed as the MNDO core-core repulsion term as shown in Eq.(4).E n A ;B ðÞ¼Z A Z B s A s A j s B s B i h 1þe Àa A R AB þe Àa B R ABÀÁð4ÞThis approximation works well for most main-group elements,but when molybdenum was being parameterized,V oityuk [14]found that the errors in heats of formation and geometries were unacceptably large,and good results were achieved only when a diatomic term was added to the core-core approximation,as shown in Eq.(5).E n A ;B ðÞ¼Z A Z B s A s A j s B s B i h 1þx AB e Àa AB R AB ÀÁð5ÞWhen PM3parameters for elements of Groups IA were being optimized,the MNDO approximation to the core-core expression was found to be unsuitable.In theseelements there is only one valence electron so the core charge is the same as that of hydrogen.A consequence of this was that the apparent size of these elements was also approximately that of a hydrogen atom,in marked contrast with observation.For these elements,diatomic core-core parameters were also found to be essential.Further examination showed that when diatomic param-eters were used,there was always an increase in accuracy;therefore,in the current work,Eq.(4)was replaced systematically by Eq.(5).As the interatomic separation increased,Voityuk ’s equation converged to the exact point-charge interaction,as expected.However,for rare gas interactions,an increase in accuracy was found when the rate of convergence was increased by the addition of a small perturbation.Subse-quently,the perturbed function was found to be generally beneficial.Because of this,the general form of the core-core interaction used in PM6is that given in Eq.(6).E n A ;B ðÞ¼Z A Z B s A s A j s B s B i h 1þx AB eÀa AB R AB þ0:0003R 6AB ðÞð6ÞAt normal chemical bonding distances,Eqs.(5)and (6)haveessentially similar behavior,but at distances of greater than about 3Åthe effect of the perturbation is to make the PM6function significantly smaller than the V oityuk approximation.d-orbitals on main-group elementsThiel and V oityuk have shown [13]that a large increase in accuracy results when d -orbitals are added to main-group elements that have the potential to be hypervalent.During preliminary stages of this work,d -orbitals were excluded from main-group elements,and the parameters were optimized.This work was then repeated but with d -orbitals on various main-group elements.The results were in accordance with Thiel ’s observation:the accuracy of the method increased significantly.Because of this,d-orbitals were added to several main-group elements:the value of the increased accuracy far outweighs the extra computational cost.The effect of the addition of d -orbitals was fundamen-tally different between main-group elements and transition metals.For main-group elements,the effect of d -orbitals is merely a perturbation:to a large degree the chemistry of these elements is determined by the s and p atomic orbitals.This is not the case with transition metals,where the d -orbitals are of paramount importance and the s and p orbitals are of only very minor significance.In recognition of the importance of the s and p shells in main-group chemistry,specific parameters are used for the five one-center two-electron integrals.Conversely,for the transition metals,the values of these integrals are derived directly from the internal orbital exponents.Unpolarizable coreAs noted earlier,the NDDO core-core interaction is a function of the number of valence electrons.For elements on the left of the periodic table these numbers are small and can cause the elements to appear to be too small.This was part of the rationale behind the adoption of V oityuk ’s diatomic core-core parameters.However,even the V oityuk approximation failed during parameter optimization when,in rare cases,a pair of atoms would approach each other very closely.Examination of these catastrophes indicated that the cause was the complete neglect of the unpolarizable core of the atoms involved.To allow for its presence,the core-core interaction for all element pairs was modified by the addition of a simple function,f AB ,based on the first term of the Lennard-Jones potential [32].A candidate function was constructed,Eq.(7),using the fact that,to a first approximation,the size of an atom increases as the third power of its atomic number.f AB¼cZ 1=3A þZ 1=3B R AB0@1A 12ð7ÞThe value of c was set to 10−8,this being the best compromise between the requirements that the function should have a vanishingly small value at normal chemical distances.That is,under normal conditions the value of the function should be negligible,and at small interatomic separations the function should be highly repulsive,i.e.,that it should represent the unpolarizable core.Individual core-core correctionsFor a small number of diatomic interactions,the general expression for the core-core interaction was modified in order to correct a specific fault.Because it is desirable to keep the methodology as simple as possible,modifications of the approximations were made only after determining that the existing approximations were inadequate.The diatomic specific modifications were:O –H and N –HIn the original MNDO formalism,the general core-core interaction,Eq.(4),was replaced in the cases of O –H and N –H pairs with Eq.(8).E n A ;B ðÞ¼Z A Z B s A s A j h s B s B i 1þR AB e ÀαA R AB þR AB e ÀαB R ABÀÁð8ÞAn unintended effect of this change was that at distances where hydrogen-bonding interactions are important,the diatomic contribution to the ΔH f is greater than if the general approximation,Eq.(4),had been used.This contributed to a reduced hydrogen-bonding interaction in MNDO,and was a contributor to the need for modified core-core interactions in AM1and PM3.In PM6,the MNDO core-core approximation is replaced by Voityuk ’s diatomic expression,but even with that modification,the resulting hydrogen bond interaction energy was too small.In an attempt to increase it,the V oityuk approximation was replaced by Eq.(9).E n A ;B ðÞ¼Z A Z B s A s A j s B s B i h 1þx AB e Àa AB R 2ABð9ÞAt normal O –H and N –H separations,approximately1Å,Eqs.(5)and (9)have similar values,but at hydrogen bonding distances,∼2Å,the contribution arising from the exponential term is significantly reduced,resulting in a corresponding increased hydrogen bond interaction energy.C –CAfter optimizing all parameters,it was found that com-pounds containing yne groups,-C ≡C-,were predicted to be too stable by about 10kcal mol −1per yne group.This error was unique to compounds with extremely short C –C distances,and in light of the increased emphasis on accurately reproducing the properties of organic com-pounds,the C –C core-core term was perturbed by the addition of a repulsive term.This term was optimized to correct the error in the yne groups and to have a negligible effect on all other C –C interactions.The optimized form of the C –C core-core interaction is given in Eq.(10).E n A ;B ðÞ¼Z A Z B s A s A j h s B s B i1þx AB eÀαAB R AB þ0:0003R 6AB ðÞþ9:28e À5:98R ABð10ÞSi –ODuring testing of PM6,neutral silicate layers of the typefound in talc,H 2Mg 3Si 4O 12,were found to be slightly repulsive instead of being slightly bound.An attempt was made to correct for this error by adding a weak perturbation to the Si –O interaction,illustrated by Eq.(11).E n A ;B ðÞ¼Z A Z B s A s A j h s B s B i1þx AB eÀαAB R AB þ0:0003R 6AB ðÞÀ0:0007e ÀR AB À2:9ðÞ2ð11Þ。
施一公团队揭示RNA最“复杂”的秘密:剪接体视频
施一公团队揭示RNA最“复杂”的秘密:剪接体视频生化教科书将剪接体形容为细胞里最复杂的超大分子复合物。
剪接体结构解析的难度普遍认为高于RNA聚合酶和核糖体,后两者的结构解析曾分别获得2006年和2009年的诺贝尔化学奖。
剪接体机器和Pre-mRNA的剪接循环剪接体是什么?我们都知道,包括人类在内的每一种生物的生命活动,包括行为、语言、认知等等,都是由基因所编码的。
那么基因是如何控制生物体的生命过程呢?那就不得不提DNA了。
众所周知,DNA是遗传物质。
但是DNA本身没有办法直接控制细胞活动,它需要将自身携带的遗传信息经过RNA转化到蛋白质,后者直接执行生命活动。
如果很难理解,不妨把DNA想象成我们的大脑,本身并不能跑不能跳,大脑(DNA)需要通过神经系统(RNA)来控制肌肉等组织器官(蛋白质)让我们能吃能动。
遗传信息从存储的DNA转化为具有各种结构、执行各种功能的蛋白质的过程,就叫做中心法则。
中心法则其实是一个遗传信息传递的过程。
在真核细胞中,该过程共分为三步,分别由RNA聚合酶、剪接体和核糖体执行。
首先,以DNA为模板,在RNA聚合酶的催化作用下合成前体信使RNA (简称pre-mRNA),DNA中的遗传信息于是传递到了pre-mRNA,这一步叫做转录;事实上,真核细胞DNA中遗传信息不是连贯的,是被很多被称为内含子的序列打乱顺序的,遗传信息实际分布在被称为外显子的片段上,而通过转录,这样的非常粗糙的“乱序”信息也跟着被传递到了pre-mRNA,等待着被筛选破译,这个过程就需要剪接体大显神威。
精确地剪掉内含子、连接外显子,生成携带有连贯信息的成熟信使RNA,这一步就叫做剪接;随后,成熟的信使RNA将作为模板,在核糖体的催化作用下合成蛋白质。
丰富多彩的蛋白质执行支撑起细胞活动的各种功能。
关于第一步的转录和第三步的核糖体翻译,科学家们已经在原子、分子层面上“看清楚”它们是如何进行的了,但是中间这一步的剪接究竟是如何完成,在2015年之前,科学家们主要通过遗传和生化研究获得相关线索和证据,去推测去猜想,但在结构和分子机理上的认知并不清楚。
sql的四个索性
sql的四个索性1. 普通索引这是最基本的索引,它没有任何限制,比如上文中为title字段创建的索引就是一个普通索引,MyIASM中默认的BTREE类型的索引,也是我们大多数情况下用到的索引。
01–直接创建索引02CREATE INDEX index_name ON table(column(length))03–修改表结构的方式添加索引04ALTER TABLE table_name ADD INDEX index_name ON (column(length))05–创建表的时候同时创建索引06CREATE TABLE `table` (07`id` int(11) NOT NULL AUTO_INCREMENT ,08`title` char(255) CHARACTER SET utf8 COLLATEutf8_general_ci NOT NULL ,09`content` text CHARACTER SET utf8 COLLATEutf8_general_ci NULL ,10`time` int(10) NULL DEFAULT NULL ,11PRIMARY KEY (`id`),12INDEX index_name (title(length))13)14–删除索引15DROP INDEX index_name ON table2. 唯一索引与普通索引类似,不同的就是:索引列的值必须唯一,但允许有空值(注意和主键不同)。
如果是组合索引,则列值的组合必须唯一,创建方法和普通索引类似。
01–创建唯一索引02CREATE UNIQUE INDEX indexName ON table(column(length)) 03–修改表结构04ALTER TABLE table_name ADD UNIQUE indexName ON (column(length))05–创建表的时候直接指定06CREATE TABLE `table` (07`id` int(11) NOT NULL AUTO_INCREMENT ,08`title` char(255) CHARACTER SET utf8 COLLATEutf8_general_ci NOT NULL ,09`content` text CHARACTER SET utf8 COLLATEutf8_general_ci NULL ,10`time` int(10) NULL DEFAULT NULL ,11PRIMARY KEY (`id`),12UNIQUE indexName (title(length))13);3. 全文索引(FULLTEXT)MySQL从3.23.23版开始支持全文索引和全文检索,FULLTEXT索引仅可用于 MyISAM 表;他们可以从CHAR、VARCHAR或TEXT列中作为CREATE TABLE语句的一部分被创建,或是随后使用ALTER TABLE 或CREATE INDEX被添加。
fulltext翻译
J Appl Electrochem (2009) 39:577-582DOI 10.1007/s10800-008-9695-zORIGINAL PAPERElectrochemical treatment of pharmaceutical azo dye amaranthfrom waste waterRajeev Jain . Nidhi Sharma . Keisham RadhapyariReceived: 25 February 2008/Accepted: 13 0ctober 2008/Published online: I November 2008@ Springer Science+Business Media B.V. 2008Abstract The electrochemical behavior of pharmaceuti-cal azo dye amaranth has been investigated in distilledwater and Britton-Robinson buffer. One well-definedirreversible cathodic peak is observed. This may beattributed to the reduction of the -N=N- group. Calculationof the number of electrons transferred in the reductionprocess has been performed and a reduction mechanismproposed. Results indicate that the electrode process isdiffusion controlled. The cathodic peak in the case of controlled potential electrolysis is found to reduce substantially with a decrease in color and absorbance. The reaction has first order kinetics with k value 5.75 x10-2 abs min-l. The effciency of different electrode materials (platinum and steel) for decolorisation is compared. Chemical oxygen demand (COD) decreases substan-tially from 2,680 t0 96 ppm at platinum and t0 142 ppm atsteel. This translates t0 97% COD removal at platinum and95% at steel.Keywords Electrochemical treatment . AmaranthAzo dye . Industrial effluents . CV, CODI IntroductionAzo dyes continue to be a source of pollution fromindustrial processes which employ dyes to color paper,R. Jain . N. Sharma . K. RadhapyariSchool of Studies in Chemistry, Jiwaji UniversityGwalior 474011, Indiae-mail: raj eevj ain54 @ yahoo.co.inplastics, foodstuffs, pharmaceutical products, and naturaland artificial fibers [1, 2]. It is reported that approximately5 tonnes of dye discharge from dye and coloration industriesevery year [3]. The release of such compounds into theenvironment is of great concern due to their toxicity,mutagenicity, carcinogenicity, and bio-transformationproducts [4-6]. Hence. much research has focused onmethods of azo dye destruction. Many treatment processeshave been investigated extensively to treat wastewaters suchas chemical precipitation [7], adsorption [8], biologicaltreatment [9], photocatalytic degradation [10, 11], electro-catalytic oxidation [12], ozonation [13], Fentons' reaction[14], and electrochemical methods [15-19]. Electrochemi-cal techniques are an attractive methodology for thetreatment of dye wastewaters. This technique has significantadvantages viz., wide application, simple equipment, easyoperation, lower temperature requirements. and no sludgeformation [20-24].Amaranth (Fig.1) [trisodium salt of l-(4-sulpho-l-naphthylazo)-2-naphthol-3, 6-disulphonic acid] is an acidicmonoazo dye used as food and pharmaceutical colorant.Only a few analytical methods, such as square waveadsorptive stripping voltammetry (SWAdSV) and spec-trophotometry are available for determination of amaranthin soft drink samples [25]. Degradation of amaranth fromenvironmental samples has been studied on activated car-bon fiber (ACF) electrodes [26,27]. The amaranth azo dyehas electroactive groups. However. its electrochemicalbehavior and treatment have not been investigated.Therefore, cyclic voltammetric and differential pulsepolarographic studies have been undertaken in the presentwork for understanding the electrochemical behavior ofamaranth. Results have been analyzed employing the cri-terion of complete decolorisation of the dye-containingsolutions.Springer578J Appl Electrochem (2009) 39:577-582Na07SOH S03NaFig. 1 Structure of amaranth2 Experimental2.1 InstrumentationO,NaCyclic voltammetric (CV) studies were carried out on an EGand G potentiostat (Princeton Applied Research) integratedwith applied electrochemistry software. The working elec-trode potential was cycled between -1.2 and +1.2 V atdifferent sweep rates (50-2.000 mV s-1). The electrochemical cell consisted of three electrodes (in close proximity) immersed in the solution to be electrolyzed. The voltammetric behavior was studied using platinum as working electrode, SCE as reference, and platinum wire as counter electrode. Controlled potential electrolysis was carried out using a cyclic voltammograph coupled to a digital electronic 2000 0mnigraph x-y/t recorder. The working electrodes used for controlled potential electrolysis (CPE) were platinum foil (3 x 3 cm2)and steel foil (4.5 x 3.5 cm2), Ag/AgCI as reference elec-trode and platinum wire as counter electrode.Differential pulse polarographic (DPP) measurementswere carried out on an Elico pulse polarograph model CL 90 connected with Polarocord recorder model LR-108.Triple distilled mercury was used for the DME. The capillary had a flow rate of 3.02 mg s-l together with a drop time of 3 s. The pH measurements were made on a Hach digital EC-40 Benchtop pH/ISE meter. The absorption spectra of samples were recorded using an Elico SL 159 UV-Visible spectrophotometer. The chemical oxygen demand (COD) was determined using the open reflux method using COD digester apparatus (Spectralab 2015-S).The synthetic azo dye amaranth was obtained from Aldrich USA. All chemicals used were AR grade. Readymade silica gel G plate for TLC, having fine coating on alumina sheet. was obtained from E-Merck.2.2 Reagents and materialsStock solution of amaranth (2 x l0-3 M) was prepared in doubly distilled water. In order to evaluate the effect of varying pH, BR buffers in the pH range 2.5-12.0 were prepared as per a literature method[,. ]. The supportingSpringerelectrolyte was l.0 M KCI.For the COD experiments reagents were prepared in accordance with standard methods (APHA. 1995) [29].2.3 ProcedureThe CV and DPP studies were carried out by mixing l.0 mL of potassium chloride, 1.0 mL stock solution, and 8.0 mL of appropriate BR buffer/distilled water. Solutions of different concentrations were prepared. Dissolved oxygen was removed from the solution by passing nitrogen gas for about 15 min. The polarograms and cyclic voltammograms were then recorded. The redox behavior was studied at varying pH (2.5-12.0), concentrations and sweep rates (100-2,000mV s-l). Controlled potential electrolysis of the dye solution was performed at slightly more negative potential than the peak potential of the respective peak. Absorbance of the olution was measured at different time intervals at 520 nm.The value of the rate constant k was calculated from the l091(absorbance) vs time plots. The number of electrons transferred was calculated from the decrease in current with time during electrolysis. Controlled potential coulometry was also carried out at different pH values. The progress of electrolysis was monitored by recording cyclic voltammograms at regular intervals of time. The end products of electrolysis were identified by TLC. For COD studies. The experiments were carried out as per standard methods.2.4 CoulometryFor the coulometric determination of number of electrons "n" consumed in the reduction. a solution of depolarizer,potassium chloride, and buffer/distilled water was mixed in the same ratio as that for CV and DPP studies. The solution was de-aerated by passing nitrogen gas for 15 min and cyclic voltammograms were recorded at slightly more negative potential than the peak potential. With the progress of electrolysis the color of the solution gradually faded and finally a colorless solution was obtained. The number of electrons "n" transferred at the platinum electrodewas determined from the formula Q = nFN.3 Results and discussion3.1 Cyclic voltammetric (CV) studiesThe cyclic voltammogram of amaranth in distilled water (2 x 10-4 M) exhibits reduction peak at -0.872 V and corresponding oxidation peak at -0.779 V at scan rate of 100 mV s-l. The cathodic peak can be safely assigned to the reduction of the azo (-N=N-) group. The separation between cathodic and anodic peak potentials is more than 60 mV. indicating the irreversible nature of the electrode process [30]. As the scan rate (v) is increased. the reduction peak potential shows negative shift and the oxidation peakpotential shows positive shift. At higher scan rate of1,000 mV s-1 the reduction peak appears at -1.0 V andthe oxidation peak at -0.697 V. The peak potential separation (AEp) increases gradually from 0.093 t0 0.306 V asthe scan rate is increased from 100 to 1,000 mV s-l suggesting the irreversible nature of the electrode process.The plot of ip,c vs 1/2 in the 6.5 pH solution is a straight line passing through the origin indicating the diffusion controlled nature of the electrode process (Fig. 2 (I)) This proportionality may be attributed to the fact that a steeper concentration gradient is established thereby increasing the rate of diffusion at faster scan rates. Reduced species will diffuse away from the electrode faster as the scan rate is increased [31,32]. A similar plot for the dye solution in neutral aqueous medium and at pH 8.8 is,once again, a linear behavior, however, not passing through the origin. This indicates adsorption effects contributing to diffusion current. In such a case an adsorbed species may undergo electron transfer.3.2 Effect of pHWell-defined cathodic peaks in the acidic pH range are obtained with both platinum and glassy carbon electrodes.With increase in pH the cathodic peak shifts negatively and the anodic peak positively with increasing pH, which indicates that proton transfer occurs as a step consecutive to an irreversible electrode process [33]. The plot of Ep.c vs pH is linear up to pH 6.5 as also above 6.5.However. the two linear segments have different slopes.The value of pH 6.5, is in accord with the pKa value.Above pH 6.5, the Ep,c becomes practically independent of pH. This indicates the reduction of unprotonated species34]. These results are presented in Table .3.3 Differential pulse polarographic studiesA single four-electron irreversible reduction peak is observed in the pH range 2.5 t0 12.0 at the mercury electrode. This may be attributed to reduction of the azo group(-N=N-). A shift in Ep,c towards more cathodic potentialwith pH, along with a break at pH 6.5, is observed. Beyondthis there is near constancy in Ep,c (Fig.3). This suggestsparticipation of protons in the rate determinatiAnalysisofthepeakEd.e.vs log刍)- O;,at4m, lo[rt]Xtand shifting of the peak potential towards more negativevalue with concentration suggests the irreversible nature ofthe electrode process [35,36]. The results of DPP studies arethus in close agreement with those of the CV studies.3.4 Controlled potential coulometric studiesBy using controlled potential coulometry, the number ofelectrons transferred "n" at platinum electrode werecalculated and this lies in the range 2土0.2 (Table ..). Thevalue of "n" lies in the range 4 + 0.2 at the steel electrode.Controlled potential electrolysis of amaranth (2 x 10-4 M)at platinum and steel electrodes was carried out at -1.20 V.Bench scale electrochemical treatment was carried out.Electrolysis results in complete disappearance of colorwhich is fairly faster in case of platinum where the solutionis decolorized within 10 min of electrolysis leading to thecomplete disappearance of the cathodic peak current. Withsteel. the colored solution took 80 min for complete colorremoval. The data are summarized in Table '_ . A compara-tive overlay of the dye solution of pH 8.8 before and afterelectrochemical treatment is presented as Fig. i.3.5 Spectral studiesUV-visible spectra of amaranth (2 x 10-4 M) in distilledwater were recorded at Amax = 532.5 nm. The progress of 有错controlled potential electrolysis was monitored by record-ing spectral changes at different time intervals. AtAnax = 532.5 nm, absorbance decreases systematically 有错with the progress of electrolysis. The kinetic measurementswere conducted at steel electrodes. The observed rateconstant, k= 5.75 x 10-2 a s min-l was determinedfrom the first-order kinetics plot of log absorbance vs time3.6 COD removalThe electrolyzed solution shows a substantial decrease inCOD from an initial value of 2,680 ppm to a final value of96 ppm at platinum and t0 142 ppm at steel.3.7 Proposed mechanismOn the basis of the coulometry, controlled potential elec-trolysis, chromatographic and spectral analysis, tworeduction mechanisms are proposed. One is the two elec-tron reduction to hydrazo compound at platinum (Fig. ).A four electron reduction at steel (Fig. ) may be favoredby the presence of the strong electron releasing substituent,the -OH group [37].In Fig.6, amaranth undergoes 2e- reduction in acidic aswell as alkaline medium. The reduction process proceedsby the protonation of the polarized molecule resulting in the formation of (B). In the second step, which is slow and rate determining, (B) accepts 2e- and a proton and results in the formation of stable hydrazo (-NH-NH-) form (C).In Fig.7, 4e- reduction takes place at the steel foil electrode. The I and II steps of reduction proceed in the same way as described in Fig. . In step III. (c) the hydrazo moiety undergoes a further two electron reduction resulting into two products (D) and (E).4 ConclusionThe newly developed electrochemical method gives satis- factory and promising results. The electrochemical reduction of amaranth under the experimental conditions described in this work is an irreversible process controlled by diffusion. Both platinum and steel electrodes exhibit great stability and resistance to redox and acidic/basic environments showing no deactivation. During the elec- trochemical degradation process, the COD decreases by approximately 97u/o at the platinum electrode and by 95% at the steel foil electrode with complete color removal. The electrochemical treatment developed achieves higher de- coloration and COD removal than that reported previously through the ACF electrode treatment method [26] which gives only 60% COD removal. Hence, the present elec- trochemical procedure is a better alternative approach for wastewater treatment resulting in significant lowering of toxicity.。
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Fulltext-ActaPalaeontologicaPolonica
New specimens of albanerpetontid amphibians from the Upper Cretaceous of UzbekistanPAVEL P.SKUTSCHASThe albanerpetontid fossil record in Asia was limited to five dentaries of unidentified genus from the Upper Cretaceous Khodzhakul(lower Cenomanian)and Bissekty(Turonian) formations,Kyzylkum Desert,Uzbekistan.Here I describe two fragmentary frontals from the Khodzhakul local fauna as the first unequivocal record of the genus Albanerpeton in Asia. IntroductionAlbanerpetontidae is a monophyletic family of fossil salaman−der−like lissamphibians,known from the Middle Jurassic–early Pliocene of Laurasia and North Africa(Fox and Naylor1982; Gardner and Averianov1998;Gardner2000a,2002;McGowan 2002;Gardner et al.2003;Venczel and Gardner2005).The phylogenetic relationships of the Albanerpetontidae with other lissamphibian groups are still uncertain:different cladistic analy−ses nest this group as the sister−taxon of all living lissamphibians (caecilians,salamanders,and frogs)or only of frogs and salaman−ders(Gardner2001;McGowan2002;Ruta et al.2003).Currently the family Albanerpetontidae includes three valid genera(Gardner et al.2003;Venzel and Gardner2005):Albanerpeton Estes and Hoffstetter,1976from the Early Cretaceous–early Pliocene de−posits of North America and Europe(seven species),Celtedens McGowan and Evans,1995from the Late Jurassic?–Early Creta−ceous of Europe(two species)and Anoualerpeton Gardner,Ev−ans,and Sigogneau−Russell,2003from the Middle Jurassic–Early Cretaceous of Europe and North Africa(two species).Curtis and Padian(1999:figs.11,12)assigned to the Caudata two atlantes from the Early Jurassic Kayenta Formation in Ari−zona,USA.These atlantes are short and have a very deep posterior cotyla(seemingly notochordal),a foramen for the first spinal nerve and a broad intercotylar tubercle flanked by shallow,weakly expanded laterally anterior cotylae.According to this complex of characters,these atlantes more likely belong to Albanerpetontidae rather than Caudata(Averianov et al.in press).If so,the Kayenta specimens extend temporal range of the group back to the Early Jurassic.The albanerpetontid fossil record in Asia was limited to five dentaries of unidentified genus from the Upper Cretaceous Khodzhakul(lower Cenomanian)and Bissekty(middle–upper Turonian)formations,Kyzylkum Desert,Uzbekistan(Nessov 1981;Gardner and Averianov1998).Here I report on new taxo−nomically informative fragments of albanerpetontid frontals ZIN PH1/78and ZIN PH2/78from the Khodzhakul local fauna collected by the Uzbek−Russian−British−American−Canadian Paleontological(URBAC)Expedition in2004(Archibald et al. 1998).Both are part of the Paleoherpetological Collection of the Zoological Institute(ZIN PH),Russian Academy of Sciences, Saint Petersburg,Russia.Both were found at site SSHD−8, Sheikhdzheili locality;Kyzylkum Desert,north−central Uzbeki−stan;upper part of Khodzhakul Formation;lower Cenomanian, Upper Cretaceous.DescriptionZIN PH1/78is a posterior fragment of fused frontals which re−tains a faint median line of fusion ventrally near the posterior edge(Fig.1A).The anterior part with internasal process and an−terior slots is not preserved.The orbital margin is relatively short and slightly concave in dorsal or ventral view.The poste−rior slot for receipt of the posterior end of the prefrontal is deep. Posterior to the posterior slot,the lateral wall of the frontal ex−tends posterolaterally about20°from the midline.The specimen is about3mm wide across the posterior edge.The posterior bor−der of the frontal roof is transverse and slightly concave on ei−ther side of the midline.The ventrolateral orbital crest is moder−ately wide in ventral view and lacks distinct grooves.There is a small triangular facet for the parietal on the posterior end of the ventrolateral orbital crest which projects slightly beyond the posterior edge of the frontal roof.The medial edge of the ventrolateral orbital crests is rather sharp and ventromedially oriented.Despite the absence of the anterior part of the bone,the short preserved part of the orbital margin diverging from the midline at a relatively large angle suggests that the bone was tri−angular in shape.ZIN PH2/78(Fig.1B)is an incomplete anterior fragment of fused frontals with a distinctly worn ventral surface.Generally, ZIN PH2/78agrees well in morphology and size with ZIN PH 1/78.Two small ventral foramina(probably for the entry of the orbitonasal artery;Gardner1999a)are present on the frontal roof,at the level of anterior edge of posterior slot.The dorsal surface of the both specimens bears a sculpture of shallow po−lygonal pits.DiscussionThe fused frontals of albanerpetontids are one of the most taxo−nomically informative elements which may be used to differenti−http://app.pan.pl/acta52/app52−819.pdf Acta Palaeontol. Pol.52 (4): 819–821, 2007ate taxa (McGowan 1998a;McGowan and Evans 1995;Gardner 2000b;Rees and Evans 2002).In Celtedens and Anoualerpeton the frontals are hourglass−shaped,nearly bell−shaped or rectangu−lar in outline,with a relatively long orbital margin (Fig.2A,B)and with narrow,grooved ventrolateral orbital crests (Gardner 2000a;Rees and Evans 2002;Gardner et al.2003).In Albanerpeton the frontals are triangular in outline,with relatively short orbital mar−gins (Fig.2C)and wide ventrolateral orbital crests without any grooves (Gardner 2000a,2002;Rees and Evans 2002).The Uzbek specimens (especially ZIN PH 1/78)strongly resemble those of Albanerpeton (presumably triangular in dorsal or ventral outline,short orbital margin,relatively wide and ungrooved ventrolateral orbital crests)and are referred to this genus.The geologically oldest record for Albanerpeton is in the lat−est Aptian or earliest Albian of Oklahoma,and subsequent Cre−taceous records of this genus were apparently restricted to the North American Western Interior and Europe (Gardner 1999b,2002; Venzel and Gardner 2005).The occurrence of Albanerpeton in lower Cenomanian of Uzbekistan raises two alternative scenarios:the Uzbek Albaner−peton may be a North American immigrant dispersed into Asia through the Bering Land Bridge during the Albian–early Ceno−manian or the genus Albanerpeton may have had an Asian ori−gin and dispersed into the North America before the latest Aptian–earliest Albian.The first scenario is more preferable at our current state of knowledge,because of the absence of Alba−nerpeton (and other albanerpetontid amphibians)in the rela−tively diverse Jurassic and Early Cretaceous terrestrial tetrapod assemblages of Asia (Gardner and Averianov 1998;Gardner 1999).An albanerpetontid frontal was reported from the Upper820ACTA PALAEONTOLOGICA POLONICA 52(4),2007Fig.1.Albanerpetontid amphibian Albanerpeton sp.ZIN PH 1/78(A )and ZIN PH 2/78(B ).Frontals from the Late Cretaceous (early Cenomanian)Sheikhdzheili locality of Kyzylkum Desert, Uzbekistan, in dorsal (A 1, B 2), lateral (A 3, B 1), and ventral (A 2, B 3) views.Jurassic of Kyrgyzstan by Nessov (1988)but this specimen was never described or figured and its location is unknown.As a re−sult,the presence of an albanerpetontid in the Jurassic of Asia has yet to be confirmed.Albanerpetontid amphibians are extremely rare components of the Khodzhakul and Bissekty local faunas.Most other verte−brates from these formations are represented by hundreds or thousands of bones whereas albanerpetontids are known by five dentaries (four from the Khodzhakul Formation and one from the Bissekty Formation)described by Gardner and Averianov (1998),an indeterminate dentary fragment from the Khodz−hakul Formation (personal observation)and the two fragments of fused frontals described herein.The reason for the rarity of the albanerpetontids in the Khodzhakul and Bissekty local fau−nas is unclear.The presence of non−endemic albanerpetontid taxon in Asia was predictable.On the basis of continental reconstructions and the limited fossil occurrences of albanerpetontids in Asia,Gard−ner and Averianov (1998)proposed that Asian albanerpetontids would show close affinities with European or North American taxa.The new Uzbek specimens provide the first record of Albanerpeton in Asia.Acknowledgements .—I thank all members of the URBAC Expedition for help.I am also grateful to Alexander O.Averianov (Zoological Institute,Russian Academy of Sciences,St.Petersburg,Russia)for read−ing an earlier version of the manuscript and useful comments,and to Su−san E.Evans (University College of London,UK),Magdalena Borsuk−Białynicka (Institute of Paleobiology,Polish Academy of Sciences,War−saw,Poland),and Andrzej Elżanowski (Department of Zoology,Univer−sity of Wrocław,Poland)for revisions and linguistic corrections.The fi−nancial support of the National Geographic Society (grants nos.5901−97and 6281−98),the National Science Foundation (grants EAR−9804771and 0207004),the Civilian Research and Development Foundation and the Russian Foundation for Basic Research (grants RU−G1−2571−ST−04,CRDF RUB1−2860−ST−07[RFBR 07−04−91110−AFGIRa])are grate−fully acknowledged.ReferencesArchibald,J.D.,Sues,H.−D.,Averianov,A.O.,King,C.,Ward,D.J.,Tsaruk,O.I.,Danilov,I.G.,Rezvyi,A.S.,Veretennikov,B.G.,and Khodjaev,A.1998.Précis of the Cretaceous paleontology,biostratigraphy and sedi−mentology at Dzharakuduk (Turonian?–Santonian),Kyzylkum Desert,Uzbekistan.In :S.G.Lucas,J.I.Kirkland,and J.W.Estep (eds.),Lower to Middle Cretaceous Terrestrial Ecosystems.Bulletin of the New Mexico Museum of Natural History and Science 14:21–28.Averianov,A.O.,Martin,T.,Skutschas,P.P.,Rezvyi,A.S.,and Bakirov,A.(in press).Amphibians from the Middle Jurassic Balabansai Svita in the Fergana Depression, Kyrgyzstan (Central Asia).Palaeontology .Curtis,K.and Padian,K.1999.An Early Jurassic microvertebrate faunafrom the Kayenta Formation of northeastern Arizona:microfaunal change across the Triassic–Jurassic boundary.PaleoBios 19: 19–37.Fox,R.C.and Naylor,B.G.1982.A reconsideration of the relationships ofthe fossil amphibian Albanerpeton .Canadian Journal of Earth Sci−ences 19: 118–128.Gardner,J.D.1999a.Redescription of the geologically youngest albaner−petontid (?Lissamphibia):Albanerpeton inexpectatum Estes and Hoff−stetter,1976,from the Middle Miocene of France.Annales de Paléonto−logie 85: 57–84.Gardner,J.D.1999b.The amphibian Albanerpeton arthridion and the Aptian–Albian biogeography of albanerpetontids.Palaeontology 42:529–544.Gardner,J.D.2000a.Revised taxonomy of albanerpetontid amphibians.Acta Palaeontologica Polonica 45: 55–70.Gardner,J.D.2000b.Albanerpetontid amphibians from the Upper Creta−ceous (Campanian and Maastrichtian)of North America.Geodiversitas 22: 349–388.Gardner,J.D.2001.Monophyly and the affinities of albanerpetontid am−phibians (Temnospondyli;Lissamphibia).Zoological Journal of the Linnean Society 131: 309–352.Gardner,J.D.2002.Monophyly and intrageneric relationships of the am−phibian Albanerpeton .Journal of Vertebrate Paleontology 22: 12–22.Gardner,J.D.and Averianov,A.O.1998.Albanerpetontid amphibians fromMiddle Asia.Acta Palaeontologica Polonica 43: 453–467.Gardner,J.D.,Evans,S.E.,and Sigogneau−Russell,D.2003.New albaner−petontid amphibians from the Early Cretaceous of Morocco and Middle Jurassic of England.Acta Palaeontologica Polonica 48: 301–319.McGowan,G.J.1998a.Frontals as diagnostic indicators in fossil albaner−petontid amphibians.Bulletin of the National Science Museum,Series C (Geology and Paleontology ) 24: 185–194.McGowan,G.J.2002.Albanerpetontid amphibians from the Lower Creta−ceous of Spain and Italy:a description and reconsideration of their sys−tematics.Zoological Journal of the Linnean Society 135: 1–32.McGowan,G.J.and Evans,S.E.1995.Albanerpetontid amphibians fromthe Cretaceous of Spain.Nature 373: 143–145.Nessov,L.A.1981.Cretaceous salamanders and frogs of Kizylkum Desert[in Russian].Trudy Zoologičeskogo Instituta Akademii Nauk SSSR 101:57–88.Nessov,te Mesozoic amphibians and lizards of Soviet MiddleAsia.Acta Zoologica Cracoviensia 31: 475–486.Rees,J.and Evans,S.E.2002.Amphibian remains from the Lower Creta−ceous of Sweden:the first Scandinavian record of the enigmatic group Albanerpetontidae.GFF 124: 87–91.Ruta,M.,Coates,M.I.,and Quicke,D.L.J.2003.Early tetrapod relation−ships revisited.Biological Reviews 78: 251–345.Venczel,M.and Gardner,J.D.2005.The geologically youngest albaner−petontid amphibian,from the lower Pliocene of Hungary.Palaeontol−ogy 48: 1273–1300.Pavel P.Skutschas [*****************],Saint−Petersburg State University,Biological Faculty,Department of Vertebrate Zoology,Universitetskaya nab.7/9, Saint Petersburg 199034, Russia.http://app.pan.pl/acta52/app52−819.pdfBRIEF REPORT 821orbital margin orbital margin orbital marginFig.2.Outlines of the fused frontals of albanerpetontids,all in ventral view.A .Celtendens sp.(modified from Gardner 2002:fig.1P).B .Anoualerpeton unicus (modified from Gardner et al.2003:fig.D2).C .Albanerpeton nexuosus (modified from Gardner 2002: fig. 1R). Scale bars 1 mm.。
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世界人权宣言(中英文对照)
世界人权宣言UniversalDeclaration of Human Rights1948年12月10日第217A(III)号决议通过1948年12月10日,联合国大会采认世界人权宣言, 请求它的会员国公布该宣言, 并"在无政治的考量下,在各级学校及教育机构里,传播、张贴、研读及解说其内容.”On December 10, 1948theGeneral Assembly of the United Nations adopted andproclaimedthe Universal Declaration of Human Rights the fulltext of wh ich appears in the following pages. Following this historic act the Assemblycalled upon all Membercountries to publicizethetext of the Declarationand”to causeit to bedisseminated,displayed, readand expounded principally in schoolsandothereducational institutions,without distinction ba sed onthepolitical status ofcountriesor territories."序言PREAMBLE鉴於对人类家庭所有成员的固有尊严及其平等的和不移的权利的承认,乃是世界自由、正义及和平的基础,Whereas recognition of the inherentdignity andof the equal and inalienabl erightsof all members ofthe human familyis the foundation of freedo m,justice and peace in theworld,鉴於对人权的忽视及侮蔑已发展为野蛮暴行, 这些暴行玷污了人类的良心,而一个人人享有言论和信仰自由并免於恐惧和匮乏的世界的来临,已被宣布为普通人民的最高愿望,Whereasdisregard andcontemptfor human rights haveresulted inbarbarous actswhichhaveoutraged the conscience of mankind,and the advent of a worldinwhichhuman beings shall enjoy freedom of speech and belief and freedom fromfearand wanthasbeen proclaimed as the highest aspirationof the commonpeople,鉴於为使人类不致迫不得已铤而走险,对暴政和压迫进行反抗,有必要使人权受法治的保护,Whereasitis essential, ifman is notto be compelledto have recourse,as a last resort,torebellion against tyrannyandoppression,that huma nrights should be protectedbythe rule of law,鉴於有必要促进各国间友好关系的发展,Whereas it is essential topromote the development of friendly relations between nations,鉴於联合国国家的人民已在联合国宪章中重申他们对基本人权、人格尊严和价值以及男女平等权利的信心,并决心促成较大自由中的社会进步和生活水平的改善,Whereasthe peoples of the United Nations havein the Charter reaffirmed their faith in fundamental human rights, inthe dignity and worth of thehumanperson and in the equal rightsof menand womenand have determined to promote social progress andbetter standards of lifein larger freedom,鉴於各会员国都已誓愿同联合国合作,以促进对人权和基本自由的普遍尊重和遵行,Whereas Member States have pledged themselves toachieve,in co-operation with the United Nations, the promotion ofuniversal respectfor andobs ervance of human rights and fundamental freedoms,鉴於对这些权利和自由的普遍了解,对於这个誓愿的充分实现, 有很大的重要性,Whereas a commonunderstandingofthese rights and freedoms is ofthe greatestimportancefor the full realization of this pledge,因此,现在,Now,Therefore,大会THEGENERAL ASSEMBL Y宣告proclaims这一世界人权宣言, 作为所有人民和所有国家努力实现的共同标准,以期每一个人和社会机构经常铭念本宣言,努力通过教诲和教育,促进对权利和自由的尊重,并通过国家的和国际的渐进措施,使这些权利和自由在各会员国本身人民及在其管辖下领土的人民中,得到普遍和有效的承认和遵行。
MySQL索引类型Normal、Unique和FullText的讲解
MySQL索引类型Normal、Unique和FullText的讲解MySQL的索引类型有普通索引(normal),唯⼀索引(unique)和全⽂索引(full text),合理使⽤索引可⼤⼤提升数据库的查询效率,下⾯是三种类型的索引的介绍normal:这是最基本的索引,它没有任何限制,MyIASM中默认的BTREE类型的索引,是我们⼤多数情况下⽤到的索引。
unique:表⽰唯⼀的,不允许重复的索引,如果该字段信息保证不会重复。
例如⾝份证号⽤作索引时,可设置为unique。
full text : 表⽰全⽂搜索的索引,仅可⽤于 MyISAM 表。
FULLTEXT ⽤于搜索很长⼀篇⽂章的时候,效果最好。
⽤在⽐较短的⽂本,切记对于⼤容量的数据表,⽣成全⽂索引是⼀个⾮常消耗时间⾮常消耗硬盘空间的做法。
mysql索引类型Normal,Unique,Full Text区别Normal:表⽰普通索引,⼤多数情况下都可以使⽤Unique:约束唯⼀标识数据库表中的每⼀条记录,即在单表中不能⽤每条记录是唯⼀的(例如⾝份证就是唯⼀的),Unique(要求列唯⼀)和Primary Key(primary key = unique + not null 列唯⼀)约束均为列或列集合中提供了唯⼀性的保证,Primary Key是拥有⾃动定义的Unique约束,但是每个表中可以有多个Unique约束,但是只能有⼀个Primary Key约束。
mysql中创建Unique约束Full Text:表⽰全⽂收索,在检索长⽂本的时候,效果最好,短⽂本建议使⽤Index,但是在检索的时候数据量⽐较⼤的时候,现将数据放⼊⼀个没有全局索引的表中,然后在⽤Create Index创建的Full Text索引,要⽐先为⼀张表建⽴Full Text然后在写⼊数据要快的很多总结,索引的类别由建⽴索引的字段内容特性来决定,通常normal最常见。
在实际操作过程中,应该选取表中哪些字段作为索引?为了使索引的使⽤效率更⾼,在创建索引时,必须考虑在哪些字段上创建索引和创建什么类型的索引,有7⼤原则:1.选择唯⼀性索引唯⼀性索引的值是唯⼀的,可以更快速的通过该索引来确定某条记录。
IBM Cognos Transformer V11.0 用户指南说明书
fulltext[1].pdf1
ORIGINAL PAPERDiversity of basidiomycetous phylloplane yeasts belonging to the genus Dioszegia (Tremellales)and description of Dioszegia athyri sp.nov.,Dioszegia butyracea sp.nov.and Dioszegia xingshanensis sp.nov.Qi-Ming Wang ÆJian-Hua Jia ÆFeng-Yan BaiReceived:9September 2007/Accepted:29November 2007/Published online:9December 2007ÓSpringer Science+Business Media B.V.2007Abstract From approximately 200basidiomycetous yeast isolates forming orange or orange-red colonies isolated from senescent leaves collected in different regions of China,29representative strains varying in their geographic distribution and ballistoconidium forming ability were selected for further phenotypic and molecular taxonomic studies.Sequence analysis of the large subunit (26S)rDNA D1/D2domain and the internal transcribed spacer (ITS)region including 5.8S rRNA from the strains resulted in the recognition of seven Dioszegia species,including four described species,namely D.aurantiaca , D.fristingensis ,D.hungarica and D.zsoltii var.zsoltii and D.zsoltii var.yunnanensis ,and three undescribed species.The three new species are described as Dioszegia athyri sp.nov.(type strain:CB 159T =AS 2.2559T =CBS 10119T ),Dioszegia butyracea sp.nov.(type strain:CB 261T =AS 2.2600T =CBS 10122T )and Dioszegia xingshanensis sp.nov.(type strain:HB 1.4T =AS 2.2481T =CBS 10120T )in the present study.Keywords Basidiomycetous yeasts ÁDioszegia ÁSpecies diversity ÁMolecular phylogenyIntroductionThe genus Dioszegia Zsolt in the Tremellales was revived and emended by Takashima et al.(2001)to accommodate both ballistoconidium and non ballis-toconidium-forming basidiomycetous yeast species which usually form orange-coloured colonies.Three species were originally included in the emended genus.Since then,the number of species in the genushas increased to ten (Bai et al.2002a ;Ina´cio et al.2005;Wang et al.2003).Recent studies on yeast isolates from plant leaves collected in different regions in China indicated that the natural distribu-tion of Dioszegia species in the phylloplane is common.Approximately 200basidiomycetous yeasts forming orange or orange-red colonies were isolated from senescent leaves collected directly from plants in different regions of China.On the basis of morphological grouping,29representative strains isolated from different plants or different places were selected for rDNA sequence analysis and physiolog-ical characterization.Seven Dioszegia species including four described and three undescribed taxa were recognized from the strains studied.The three new species are described as Dioszegia athyri sp.nov.,Dioszegia butyracea sp.nov.and Dioszegia xingshanensis sp.nov.in the present study.Q.-M.Wang (&)ÁJ.-H.Jia ÁF.-Y.BaiSystematic Mycology and Lichenology Laboratory,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China e-mail:wangqm@Antonie van Leeuwenhoek (2008)93:391–399DOI 10.1007/s10482-007-9216-9Materials and methodsYeast strainsThe strains studied are listed in Table1.They were isolated from senescent plant leaves using the improved ballistoconidia-fall method as described by Nakase and Takashima(1993).The leaves were collected from August to October during the years 1998–2004.All were still attached to the plants when collected.Morphological,physiological and biochemical characterizationMost of the morphological,physiological and bio-chemical characteristics were examined according to standard methods(Yarrow1998).Extraction,purifi-cation and identification of ubiquinones were carried out according to Yamada and Kondo(1973).Assim-ilation of nitrogen compounds was investigated on solid media with starved inoculum(Nakase and Suzuki1986).Table1Yeast strains studiedSpecies Strain SourceD.aurantiaca CB166Brachybotrys paridiformis,Changbai Mountain,Jilin,northeast ChinaCB170Brachybotrys paridiformis,Changbai Mountain,Jilin,northeast ChinaCB323Leptolepidium sp.,Changbai Mountain,Jilin,northeast ChinaHX4.4Ranunculus sp.,Xingshan,Hubei,central ChinaHX6.2Smilax stans,Xingshan,Hubei,central ChinaHX9.2Viburnum betulifolium,Xingshan,Hubei,central ChinaHX12.13Lindera communis,Xingshan,Hubei,central ChinaHX13.13Decaisnea fargesii,Xingshan,Hubei,central ChinaHX20.10Porana henryi,Xingshan,Hubei,central ChinaHX21.6Artemisia sp.,Xingshan,Hubei,central ChinaSH67Ligustrum sp.,Taigu,Shanxi,north ChinaD.fristingensis SH47Rhododendron oroedoxa,Taigu County,Shanxi,north ChinaXJ4E3Rubus sachalinensis,Xinjiang,northwest ChinaD.hungarica CB145Tilia amurensis,Changbai Mountain,Jilin,northeast ChinaCB247Dryopteris sp.,Changbai Mountain,Jilin,northeast ChinaHX21.3Artemisia sp.,Xingshan County,Hubei,central ChinaSH24Salix yanbianica,Taigu County,Shanxi,north ChinaD.zsoltii var.zsoltii BH4Deutzia sp.,Baihua Mountain,Beijing,north ChinaBH19Betula platyphylla,Baihua Mountain,Beijing,north ChinaCB167Brachybotrys paridiformis,Changbai Mountain,Jilin,northeast ChinaST5.13Zanthoxylum sp.,Taibai Mountain,Shaanxi,northwest ChinaSH12Vitex negundo,Taigu,Shanxi,north ChinaD.zsoltii var.yunnanensis BH3Deutzia sp.,Baihua Mountain,Beijing,north ChinaD.athyri sp.nov.CB159T Athyrium sp.,Changbai Mountain,Jilin,northeast ChinaD.butyracea sp.nov.CB261T Betula ermanii,Changbai Mountain,Jilin,northeast ChinaCB320Leptolepidium sp.,Changbai Mountain,Jilin,northeast ChinaSB4.7Euonymus verrucosoides,Taibai County,Shaanxi,northwest ChinaXJ7E2Betula platyphylla,Xinjiang,northwest ChinaD.xingshanensis sp.nov.HB1.4T Artemisia sp.,Xingshan County,Hubei,central ChinaSequencing and molecular phylogenetic analysis Nuclear DNA was extracted by using the method of Makimura et al.(1994).ITS(including5.8S rDNA) and26rDNA D1/D2domain sequences were deter-mined as described previously(Bai et al.2002b). Sequences were aligned with the Clustal X program (Thompson et al.1997).Phylogenetic trees were constructed from the evolutionary distance data cal-culated from Kimura’s two parameter model(Kimura 1980)by using the neighbor-joining method(Saitou and Nei1987).Bootstrap analyses(Felsenstein1985) were performed from1,000random resamplings.Results and discussionSeven species belonging to the genus Dioszegia were recognized among the29representative strains on the basis of D1/D2domain and ITS region sequence analysis(Table1).Eleven isolates from north,north-east and central China were assigned to D.aurantiaca. Their sequences matched that of the type(JCM2956) of the species in the ITS region and differed by zero to two substitutions in the D1/D2domain.All isolates formed ballistoconida.The present study shows that D.aurantiaca is widespread in nature and that its geographic distribution may not be restricted to colder regions as suggested by Ina´cio et al.(2005).The species was frequently isolated in Xingshan,Hubei Province,a subtropical area of central China(Table1).Strains SH47and XJ4E3isolated in north and northwest China were assigned to D.fristingensis. This species was described recently based on a single isolate from Germany(Ina´cio et al.2005).Strains SH 47and XJ4E3differed from each other by three substitutions in each of the D1/D2and ITS region. They differed from the type(PYCC5861)by one or two substitutions in the D1/D2and two orfive mismatches in the ITS region.Whereas D.fristing-ensis was originally described as producing ballistoconidia abundantly(Ina´cio et al.2005),the two Chinese isolates only rarely formed ballistocon-idia.The species was not found among phylloplane yeasts identified in Portugal(Ina´cio et al.2005).Four isolates from central,north and northeast China belonged to D.hungarica.They were identical to the type in the D1/D2domain but differed by two substitutions in the ITS region.This species contains both ballistoconidium and non ballistoconidium-form-ing strains(Takashima et al.2001).Among the four Chinese representatives of D.hungarica,ballistocon-idia were observed only in strain HX21.3on artificial media.Six strains isolated from north,northeast and northwest China were assigned to D.zsoltii based on their sequence identity to the type in the D1/D2domain. This species was originally described based on six isolates from the subtropical part of Yunnan Province in southwest China(Bai et al.2002a).Two varieties that differed by three substitutions in the ITS regions were recognized based on their intermediate DNA–DNA relatedness values(45–55%).Of the six strains of D.zsoltii identified in the present study,strain HB3 was assigned to D.zsoltii var.yunnanensis based on a matching ITS sequence.The otherfive strains were assigned to D.zsoltii var.zsoltii.Three(BH4,CB167 and SH12)had identical ITS sequences to the type, whereas strains HB19and ST5.13differed by one substitution in that region.This species was also identified among phylloplane yeasts in Portugal(Ina´cio et al.2005).D.zsoltii therefore appears to be widely distributed across areas with different climate types.Analysis of D1/D2and ITS sequences(Fig.1) placed strain CB159in a clade that included also D.takashimae,D.catarinonii,D.zsoltii,an unnamed strain Dioszegia sp.TY-217,and‘Cryptococcus hun-garicus’CBS6576with strong bootstrap support.In the D1/D2domain,strain CB159differed from ‘C.hungaricus’CBS6576by28mismatches and from the other four taxa by three to six substitutions. Differences of13–20mismatches were found with the latter in the ITS region.On this basis,we regard strain CB159as representative of a novel species,for which the name D.athyri sp.nov.is proposed.The four strains CB261,CB320,SB4.7,and XJ7E2 possessed identical ITS sequences and thefirst two, from northeast China,differed from the last two,from northwest China,by one substitution in the D1/D2 region.We interpret this as evidence that the four strains are conspecific and distinct from D.statzelliae and D.fristingensis(Fig.1).The isolates differ from the types of the two described species byfive and nine mismatches in the D1/D2and by13and26mismatches in the ITS regions,respectively.We propose the name D.butyracea sp.nov.for this new species.Strain HB1.4from Hubei Province,central China, occupied a basal position(Fig.1)with respect toD.crocea,D.aurantiaca and D.changbaiensis,with which it formed a well supported clade in the tree drawn from ITS sequences(bootstrap=94%,not shown).The strain differed from D.crocea,D.aur-antiaca,and D.changbaiensis by11,13and23 mismatches in the D1/D2domain,respectively,and by24,23and18mismatches in the ITS region.These data support our proposal of a new species,which we name D.xingshanensis sp.nov.Latin diagnosis of Dioszegia athyri Q.M.Wang&F.Y.Bai sp.nov.In YM(Difco)liquido post dies7ad17°C,cellulae vegetativae ellipsoideae,ovoideae,2.5–695–10l m,singulae aut binae.Sedimentum formatur.Post unummensem ad17°C,annulus et sedimentum formantur.Inagaro YM post unum mensem ad17°C,culturaaurantiaca,glabra,nitida,butyracea,margine glabra.Pseudomycelium non formantur.Ballistosporae ellip-soideae,ampulliformes,4–695–10l m.Fermentationulla.Glucosum,galactosum,saccharosum,maltosum,cellobiosum,trehalosum,melibiosum,raffinosum,melezitosum,inulin(lente et exigue),amylum solubile(lente et exigue),D-xylosum,L-arabinosum,D-arabino-sum(lente et exigue),D-ribosum(lente et exigue), L-rhamnosum(lente et exigue),galactitolum(exigue),D-glucitolum(lente et exigue),methyl a-D-glucosidum (lente et exigue)et salicinum(lente et exigue)assim-ilantur at non L-sorbosum,lactosum,D-glucosaminum,methanolum,ethanolum,glycerolum,erithritolum,ribitolum,D -mannitolum,acidum DL -lacticum,acidum succinicum,acidum citricum,inositolum nec hexdecanum.Ammonium sulfatum,natrum nitrosum et L -lysinum assimilantur at non kalium nitricum,ethylaminum nec cadaverinum.Vitamina externa ad crescentiam necessaria sunt.Maxima temperatura cres-centiae:29°C.Materia amyloidea iodophila non formatur.Urea finditur.Diazonium caeruleum B pos-itivum.Ubiquinonum majus :Q-10.Typus :Isolatus ex folio Athyrium sp.,CB 159T ,depositus in collectione China General Microbiological Culture Collection Center,Academia Sinica (AS 2.2559T =CBS 10119T ).Description of Dioszegia athyri Q.M.Wang &F.Y.Bai sp.nov.In YM broth,after 7days at 17°C,cells are ovoid,ellipsoidal, 2.5–695–10l m and single or pairs,budding is polar,sediment is formed (Fig.2).After 1month at 17°C,a ring and sediment are present.On YM agar,after 1month at 17°C,the streak culture is orange,butyrous,smooth,shining.The margin is entire.In Dalmau plate culture on corn meal agar,pseudohyphyae are not formed.Ballistoconidia are napiform or ellipsoidal,4–695–10l m.Fermenta-tion is negative.Glucose,galactose,sucrose,maltose,cellobiose,trehalose,melibiose,raffinose,melezitose,inulin (delayed and weak),soluble starch (delayed and weak),D -xylose,L -arabinose,D -arabinose (delayed and weak),D -ribose (delayed and weak),L -rhamnose (delayed and weak),galactitol (weak),D -glucitol (delayed and weak),methyl a -D -glucoside (delayed and weak)and salicin (delayed and weak)are assim-ilated.L -sorbose,lactose,D -glucosamine,methanol,ethanol,glycerol,erythritol,ribitol,D -mannitol,DL -lactic acid,succinic acid,critic acid,inositol and hexdecane are not assimilated.Ammonium sulfate,sodium nitrite and L -lysine are assimilated.Potassium nitrate,ethylamine hydrochloride and cadaverine dihydrochloride are not assimilated.Maximum growth temperature is 29°C.Growth in vitamin-free medium is negative.Starch-like substances are not produced.Growth on 50%(w/w)glucose-yeast extract agar is negative.Urease activity is positive.Diazonium Blue B reaction is positive.The major ubiquinone is Q-10.The type strain of Dioszegia ,CB 159T ,was isolated from a senescent leaf of Athyrium sp.collected in Changbai Mountain,Jilin province,China in October 1998.This strain has been deposited in the China General Microbiological Culture Collection Center (CGMCC),Academia Sinica,Beijing,China,as AS 2.2559T ,and in the Centraalbureau voor Schimmel-cultures,Utrecht,The Netherlands,as CBS 10119T.Fig.2Dioszegia athyri sp.nov.CB 159T vegetative cells grown in YM broth for 7days at 17°C (left)and ballistoconidia produced on corn meal agar after 14days at 17°C (right).Bars,10l mEtymology:the specific epithet athyri refers to plant genus Athyrium from which the type strain of the species was isolated.Physiologically,D.athyri sp.nov.differs from its phylogenetically close relatives D.catarinonii ,D.takashimae ,and D.zsoltii by its inability to assimilate succinic acid,DL -lactate and inositol.Latin diagnosis of Dioszegia butyracea Q.M.Wang &F.Y.Bai sp.nov.In YM (Difco)liquido post dies 7ad 17°C,cellulae vegetativae ellipsoideae,ovoideae,3–695–12l m,singulae or binae.Annulus et sedimentum formantur.Post unum mensem ad 17°C,annulus,pellicula et sedimentum formantur.In agaro YM post unum mensem ad 17°C,cultura aurantiaca,glabra,nitida,butyracea,margine glabra.Mycelium formantur vel non.Ballistosporae ellipsoideae ad ovoideae,4–595–10l m.Fermentatio nulla.Glucosum,galac-tosum,saccharosum (exigue),maltosum,cellobiosum,trehalosum,melibiosum,raffinosum,melezitosum,inulin,D -xylosum,L -arabinosum,D -arabinosum,L -rhamnosum (exigue),D -glucosaminum (lente et exigue),galactitolum,D -mannitolum et methyl a -D -glucosidum (exigue)assimilantur at non L -sorbo-sum,lactosum,amylum solubile,D -ribosum,methanolum,ethanolum,glycerolum,erithritolum,ribitolum,D -glucitolum,salicinum,acidum DL -lacti-cum,acidum succinicum,acidum citricum,inositolum nec hexdecanum.Ammonium sulfatum,natrum nitro-sum et L -lysinum assimilantur at non kalium nitricum,ethylaminum nec cadaverinum.Vitamina externa ad crescentiam necessaria sunt.Maxima temperatura crescentiae :25°C.Materia amyloidea iodophila formatur.Urea finditur.Diazonium caeruleum B positivum.Ubiquinonum majus :Q-10.Typus:Isolatus ex folio Betula ermanii Cham.,CB 261T ,depositus in collectione China General Microbiological Culture Collection Center,Academia Sinica (AS 2.2600T =CBS 10122T ).Description of Dioszegia butyracea Q.M.Wang &F.Y.Bai sp.nov.In YM broth,after 7days at 17°C,cells are ovoid,ellipsoidal,3–695–12l m and single or pairs,bud-ding is polar,a ring and sediment is formed (Fig.3).After 1month at 17°C,a ring,pellicle and sediment are present.On YM agar,after 1month at 17°C,the streak culture is orange,butyrous,smooth,shining.The margin is entire.In Dalmau plate culture on corn meal agar,true hyphae are formed or not.Ballistoconidia are ellipsoidal,ovoid.4–595–10l m.FermentationisFig.3Dioszegiabutyracea sp.nov.CB 261T vegetative cells grown in YM broth for 7days at 17°C (left)andballistoconidia produced on corn meal agar after 7days at 17°C (right).Bars,10l mnegative.Glucose,galactose,sucrose(weak),maltose,cellobiose,trehalose,melibiose,raffinose,melezitose,inulin,D-xylose,L-arabinose,D-arabinose,L-rhamnose(weak),D-glucosamine(delayed and weak),galactitol, D-mannitol and methyl a-D-glucoside(weak)are assim-ilated.L-sorbose,lactose,soluble starch,D-ribose,methanol,ethanol,glycerol,erythritol,ribitol,D-gluci-tol,salicin,DL-lactic acid,succinic acid,critic acid,inositol and hexdecane are not assimilated.Ammoniumsulfate,sodium nitrite and L-lysine are assimilated.Potassium nitrate,ethylamine hydrochloride and cadav-erine dihydrochloride are not assimilated.Maximumgrowth temperature is25°C.Growth in vitamin-freemedium is negative.Starch-like substances are pro-duced.Growth on50%(w/w)glucose-yeast extract agaris negative.Urease activity is positive.Diazonium BlueB reaction is positive.The major ubiquinone is Q-10.The type strain,CB261T,was isolated from a senescentleaf of Betula ermanii Cham.collected in ChangbaiMountain,Jilin province,China,in October1998.Thisstrain has been deposited in the China General Micro-biological Culture Collection Center(CGMCC),Academia Sinica,Beijing,China,as AS2.2600T,andin the Centraalbureau voor Schimmelcultures,Utrecht,The Netherlands,as CBS10122T.Etymology:the specific epithet butyracea refers tothe butyrous colony texture of the strains of thisspecies.Phenotypically,Dioszegia butyracea sp.nov.dif-fers from its closest relative D.statzalliae by its abilityto assimilate inulin and its inability to assimilate D-glucitol and DL-lactate.The inulin utilizing ability can also differentiate the new species from all the otherdescribed Dioszegia species(Table2).Latin diagnosis of Dioszegia xingshanensisQ.M.Wang&F.Y.Bai sp.nov.In YM(Difco)liquido post dies7ad17°C,cellulaevegetativae ellipsoideae,ovoideae,2–594–10l m,singulae or binae.Sedimentum formatur.Post unummensem ad17°C,annulus et sedimentum formantur.Inagaro YM post unum mensem ad17°C,culturaaurantiaca,glabra,nitida,butyracea,margine glabra.Pseudomycelium non formantur.Ballistoconidia napi-forma aut ellipsoidea,4–695–10l m.Fermentationulla.Glucosum,galactosum(exigue),saccharosum,maltosum,cellobiosum,trehalosum,melibiosum,raffinosum,melezitosum,D-xylosum,L-arabinosum,Table2Diagnostic physiological characters of taxa in the genus DioszegiaTaxon Assimilation of St Bc25°C1234567891011D.athyri sp.nov.+lw lw lw--lw lw----++ D.butyracea sp.nov.++---+-----+++ D.xingshanensis sp.nov.+----+-w--w+++ D.aurantiaca a+-l w-++l w+-++-D.buhagiarii b---+l+l+-+-+-+ D.catarinonii b+--+,l--,l-++,l+-,l++,-+ D.changbaiensis c+-+--l l+-+-+-+ D.crocea a+-w+++++++-+++ D.fristingensis b+--l l++l l+-++-D.hungarica a----++++-++++,-+ D.statzelliae d w--w w++w+w-+--D.takashimae b+--+,l-+,l-++,l+l+++ D.zsoltii var.zsoltii e+-+--+(w)-+,--+,w-w++ D.zsoltii var.yunnanensis e+-+----+,--(w)+,w-w++Data from a Takashima et al.(2001);b Ina´cio et al.(2005);c Wang et al.(2003);d Thomas-Hall et al.(2002);and e Bai et al.(2002a) Abbreviations:1,melibiose;2,inulin;3,soluble starch;4,Ribose;5,ribitol;6,D-mannitol;7,D-glucitol;8,salicin;9,lactate;10, succinate;11,inositol;St,starch-like substance formation;Bc,ballistoconidia formation;25°C,growth at25°C;+,positive;l, delayed positive;w,weakly positive;lw,delayed and weakly positiveD -arabinosum,L -rhamnosum,galactitolum (lente etexigue),D -mannitolum (exigue),methyl a -D -glucosi-dum (exigue),salicinum (exigue)et inositolum (exigue)assimilantur at non L -sorbosum,lactosum,inulin,amylum solubile,D -ribosum,D -glucosaminum,methan-olum,ethanolum,glycerolum,erithritolum,ribitolum,D -glucitolum,acidum DL -lacticum,acidum succinicum,acidum citricum nec hexdecanum.Ammonium sulfa-tum,natrum nitrosum et L -lysinum assimilantur at non kalium nitricum,ethylaminum nec cadaverinum.Ad crescentiam vitamina non necessaria sunt.Maxima temperatura crescentiae :26°C.Materia amyloidea iodophila formatur.Urea finditur.Diazonium caeru-leum B positivum.Ubiquinonum majus :Q-10.Typus :Isolatus ex folio Artemisia sp.,HB 1.4T ,depositus in collectione China General Microbiological Culture Collection Center,Academia Sinica (AS 2.2481T =CBS 10120T ).Description of Dioszegia xingshanensis Q.M.Wang &F.Y.Bai sp.nov.In YM broth,after 7days at 17°C,cells are ovoid,ellipsoidal,2–594–10l m and single or pairs,bud-ding is polar or bipolar,sediment is formed (Fig.4).After 1month at 17°C,a ring and sediment are present.On YM agar,after 1month at 17°C,the streak cultureis orange,butyrous,smooth,shining.The margin is entire.In Dalmau plate culture on corn meal agar,true or pseudohyphae are not formed.Ballistoconidia are ellipsoidal,napiform,4–695–10l m.Fermentation is negative.Glucose,galactose (weak),sucrose,malt-ose,cellobiose,trehalose,melibiose,raffinose,melezitose,D -xylose,L -arabinose,D -arabinose,L -rhamnose,galactitol (delayed and weak),D -mannitol (weak),methyl a -D -glucoside (weak),salicin (weak)and inositol (weak)are assimilated.L -sorbose,lactose,inulin,soluble starch,D -ribose,D -glucosamine,meth-anol,ethanol,glycerol,erythritol,ribitol,D -glucitol,DL -lactic acid,succinic acid,critic acid and hexdecane are not assimilated.Ammonium sulfate,sodium nitrite and L -lysine are assimilated.Potassium nitrate,ethyl-amine hydrochloride and cadaverine dihydrochloride are not assimilated.Maximum growth temperature is 26°C.Growth in vitamin-free medium is positive (delayed and weak).Starch-like substances are pro-duced.Growth on 50%(w/w)glucose-yeast extract agar is negative.Urease activity is positive.Diazonium Blue B reaction is positive.The major ubiquinone is Q-10.The type strain,HB 1.4T ,was isolated from a senescent leaf of Artemisia sp.collected in Hubei province,China in October 2002.This strain has been deposited in the China General Microbiological Culture Collection Center (CGMCC),Academia Sinica,Beijing,China,as AS 2.2481T and intheFig.4Dioszegiaxingshanensis sp.nov.HB 1.4T vegetative cells grown in YM broth for 7days at 17°C (left)andballistoconidia produced on corn meal agar after 14days at 17°C (right).Bars,10l mCentraalbureau voor Schimmelcultures,Utrecht,The Netherlands,as CBS10120T.Etymology:the specific epithet xingshanensis refers to the geographic origin of the type strain of this species.D.xingshanensis sp.nov.is physiologically sim-ilar to D.butyracea sp.nov.,however,the former can be distinguished from the latter by its inability to assimilate inulin(Table2).Acknowledgements This study was supported by grant no. 30470005from the National Natural Science Foundation of China(NSFC)and the Youth Association of the Institute of Microbiology(IMYA),Chinese Academy of Sciences. ReferencesBai FY,Takashima M,Jia JH,Nakase T(2002a)Dioszegia zsoltii sp.nov.,a new ballistoconidium-forming yeast species with two varieties.J Gen Appl Microbiol48:17–23 Bai FY,Zhao JH,Takashima M,Jia JH,Boekhout T,Nakase T (2002b)Reclassification of the Sporobolomyces roseus and Sporidiobolus pararoseus complexes,with the description of Sporobolomyces phaffii sp.nov.Int J Syst Evol Microbiol52:2309–2314Felsenstein J(1985)Confidence limits on phylogenies:an approach using the bootstrap.Evolution39:783–791Ina´cio J,Portugal L,Spencer-Martins I,Fonseca A(2005) Phylloplane yeasts from Portugal:seven novel anamor-phic species in the Tremellales lineage of the Hymenomycetes(Basidiomycota)producing orange-col-oured colonies.FEMS Yeast Res5:1167–1183Kimura M(1980)A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences.J Mol Evol16:111–120Makimura K,Murayama YS,Yamaguchi H(1994)Detection of a wide range of medically important fungi by the polymerase chain reaction.J Med Microbiol40:358–364 Nakase T,Suzuki M(1986)Bullera megalospora,a new spe-cies of yeast forming large ballistospores isolated from dead leaves of Oryza sativa,Miscanthus foliicola,and Sasa sp.in Japan.J Gen Appl Microbiol32:225–240 Nakase T,Takashima M(1993)A simple procedure for the high frequency isolation of new taxa of ballistosporous yeasts living on the surfaces of plants.RIKEN Rev3: 33–34Saitou N,Nei M(1987)The neighbor-joining method:a new method for reconstructing phylogenetic trees.Mol Biol Evol4:406–425Takashima M,Deak T,Nakase T(2001)Emendation of Dioszegia with redescription of Dioszegia hungarica and two new combinations,Dioszegia aurantiaca and Dios-zegia crocea.J Gen Appl Microbiol47:75–84Thomas-Hall S,Watson K,Scorzetti G(2002)Cryptococcus statzelliae sp.nov.and three novel strains of Cryptococ-cus victoriae,yeasts isolated from Antarctic soils.Int J Syst Evol Microbiol52:2303–2308Thompson JD,Gibson TJ,Plewniak F,Jeanmougin F,Higgins DG(1997)The Clustal X windows interface:flexible strategies for multiple sequence alignment aided by quality analysis tools.Nucleic Acids Res24:4876–4882 Wang QM,Bai FY,Zhao JH,Jia JH(2003)Dioszegia changbaiensis sp.nov.,a basidiomycetous yeast species isolated from northeast China.J Gen Appl Microbiol 49:295–299Yamada Y,Kondo K(1973)Coenzyme Q system in the classification of the yeast genera Rhodotorula and Cryp-tococcus and the yeast like genera Sporobolomyces and Rhodosporidium.J Gen Appl Microbiol19:59–77 Yarrow D(1998)Methods for the isolation,maintenance and identification of yeasts.In:Kurtzman CP,Fell JW(eds) The yeasts,a taxonomic study,4th edn.Elsevier, Amsterdam,pp77–100。
mysql fulltext用法
mysql fulltext用法MySQL中的`FULLTEXT`索引和全文搜索功能允许你在文本数据上执行更复杂和灵活的搜索操作。
以下是关于MySQL `FULLTEXT`的用法的一些建议:1. 创建FULLTEXT索引:在你的表上使用FULLTEXT索引,你可以对文本列执行全文搜索。
这通常用于对文章、评论或其他包含文本信息的列进行搜索。
```sqlCREATE TABLE your_table (id INT PRIMARY KEY,content TEXT,FULLTEXT (content));```2. 执行全文搜索查询:使用`MATCH`和`AGAINST`关键字执行全文搜索查询。
以下是一个简单的例子:```sqlSELECT * FROM your_table WHERE MATCH(content) AGAINST('search term');```这将返回包含与"search term"相关的内容的行。
3. 指定搜索模式:你可以指定搜索模式,例如`IN BOOLEAN MODE`。
这允许你使用布尔运算符(AND、OR、NOT)来定制搜索。
```sqlSELECT * FROM your_table WHERE MATCH(content) AGAINST('search term' IN BOOLEAN MODE);```4. 指定最小词长度:默认情况下,`FULLTEXT`搜索忽略较短的词。
你可以通过设置`ft_min_word_len`参数来更改最小词长度。
```sqlSET GLOBAL ft_min_word_len = 3;```5. 排除特定词:你可以使用`-`来排除包含特定词的行。
```sqlSELECT * FROM your_table WHERE MATCH(content) AGAINST('search term -exclude');```6. 使用通配符:你可以在搜索中使用通配符,但请注意,这可能会影响性能。
世界营销期刊排名
应新收一些学生的需要,将市场领域相关国际期刊的排名列在这里,新入门的博士生,请在半年之内,地毯式地读完前五名期刊所有文章,一年之内,读完下列期刊中五年内与研究相关的所有文章。
The number on the left is a rank based on the number ofcitations in a sam ple of 109 Ph. D. Seminar syllabi from 40respondent schools.1Journal ofMarketing2Journal ofConsumer Research3Journal ofMarketing Research4MarketingScience5Journal of theAcademy of Marketing Science6ManagementScience7Journal ofPersonality and Social Psychology8 Psychological Bulletin9StrategicManagement Journal10 Harvard Business Review11PsychologicalReview12 American Psychologist13Journalof International Business Studies14 Academy of Management Review15InternationalJournal of Research in Marketing16 Annual Review of Psychology17 Administrative Science Quarterly18Journalof Business18StructuralEquation Modeling20 American Economic Review20Journalof Retailing22 Advances in Consumer Research 23MarketingLetters24Journal ofServices Marketing25Academyof Management Journal25Journal ofConsumer Psychology27Journalof Business Research27 Rand Journal of Economics29AMA Proceedings29 Journal of Political Economy29 Quarterly Journal of Economics32Journalof Macromarketing33Econometrica33Journalof Advertising Research35Journalof Service Research36 American Journal of Sociology36 Journal of Economic Perspectives 36Journalof Public Policy & Marketing 36Public OpinionQuarterly40 Journal of Applied Psychology40 Psychological Science40SloanManagement Review43 American Sociological Review43BusinessHorizons43 Educational & Psychological Measurement43 Science47 Journal of Management47OrganizationScience47 Peronsality and Social Psychology Bulletin47 Philosophy of the Social Sciences47 Psychometrika47 Psychological Methods53 Journal of Business Logistics54DecisionSciences54Journalof Experimental Psychology: Learning, Memory andCognition54 Multivariate Behavioral Research54 Operations Research相关资源:Armstrong, J. Scott (2004), "Does an Academic Research PaperContain Use ful Knowledge? No (p<.05),"AustralasianMarketing Journal, 12 (2),62-63 [Full Text].Bauerly, Ronald J. and Don T. Johnson (2005), "An Evaluation ofJournals U sed in Doctoral Marketing Programs,"Journal of theAcademy of Marketing Sc ience,33(3), 313-329 [Rankings OnlineHere].Baumgartner, Hans and Rik Pieters (2000), "Theinfluence of marketing journ als : a citation analysis of thediscipline and its sub-areas",Center for Econo micResearch Working Paper No. 2000-123,Tilburg[FullText].Everett, James E. and Antony Pecotich (1991), "A CombinedLoglinear/MDS Model for Mapping Journals by Citation Analysis, "Journal of the American Society for Information Science, 42 (6),405-413.Hawes, Jon M., and Bruce Keillor(2002),"Assessing marketing journals: amis sion-based approach," Journal of the Academy of BusinessEducation 3 (2),70-86.Helm, Amanda E., David Hunt, and Mark B. Houston (2003),"Marketing Jour nals: Ranking the Impact of Articles, Scholars, andInstitutions," in American Marketing Association Summer Educators'Conference.Hennig-Thurau, Thorsten, Gianfranco Walsh, und Ulf Schrader(2004): VHB-J OURQUAL: Ein Ranking vonbetriebswirtschaftlich-relevanten Zeitschriften auf der Grundlagevon Expertenurteilen, in: Zeitschrift f黵betriebswirtschaftlicheFo rschung, 56 (September), 520-545 [RankingsOnline Here] [Methodologyin En glish by Gianfranco Walsh].Hult, G. Tomas M., William T. Neese, and R. Edward Bashaw(1997), "Facul ty Perceptions of Marketing Journals,"Journal ofMarketing Education,19(1), 3 7-52 [RankingsOnline Here].Koojaroenprasit, Narong, Art Weinstein, William C. Johnson, andDavid O. R emington (1998) "Marketing journal rankings revisited:Research findings and academic implications,"MarketingEducation Review, 8 (1), 95-102. McWilliams, A., D. S. Siegel and D. D. Van Fleet (2005),"Scholarly Journals as Producers of Knowledge: Theory and EmpiricalEvidence Based on Data Envelopment Analysis, "OrganizationalResearch Methods, 8 (2), 185-201. Mort, Gillian Sullivan Mort, Janel R. McColl-Kennedy, GeoffreyKiel and Geoffr ey N. Soutar (2004), "Perceptionsof Marketing Journals by Senior Academic s in Australia and NewZealand,"AustralasianMarketing Journal, 12 (2), 51-61 [FullText].Pecotich, Antony and James E. Everett (1989),"An Extension of the Citation Analysis of Selected MarketingJournals,"InternationalJournal of Research in Marketing, 6,199-204.Polonsky, Michael J. (2004), "Journal Rankings: Does One SizeFit All?" (200 4),AustralasianMarketing Journal, 12(2), 64-66 [FullText].Polonsky, Michael J. and Paul Whitelaw (2005), "What Are WeMeasuring W hen We Evaluate Journals?"Journal ofMarketing Education, 27 (2), 189-201.Polonsky, Michael J. and Paul Whitelaw, "A Multi-dimensionalexamination of Marketing Journal Rankings by North AmericanAcademics,?MarketingEducatio n Review, Forthcoming.Starbuck, William H (2005), "How Much Better Are theMost-Prestigious Jour nals? The Statistics of Academic Publication,"OrganizationScience, 16 (2), 18 0-200 [Related Materials on theAuthor's Home Page].Theoharakis, Vasilis and Andrew Hirst (2002), "PerceptualDifferences of Mar keting Journals: A Worldwide Perspective,"MarketingLetters, 13(4), 389-402 [RankingsOnline Here].Uncles, Mark D. (2004), "Journal Rankings: How Much CredenceShould We Give Them?"AustralasianMarketing Journal, 12 (2), 67-72 [FullText].Welcome To Download !!!欢迎您的下载,资料仅供参考!。
多维阅读第12级—Inside-out Skeletons 神奇的“铠甲”
Read and answer
• Animals and their exoskeletons 4. How are the exoskeletons made?
chalk, chitin
5. How do exoskeletons help animals?
Read and answer
• Animals and their exoskeletons 1. Are skeletons important? Why?
support, move, protect…
2. What is an exoskeleton? …on the outside…
3. What animals have exoskeletons?
When the old one falls off, the new one is still________ . It will not get ________ for a few days. During the days, the animal needs to be very ________.
soldier's uniform
Assignments (Choose one)
1. 向家长介绍本课所学的主要内容,即哪些动物拥有外壳、外壳 的构成,以及外壳是如何发挥作用的;亦可制作海报进行展示;
2. 课后和小组成员一起利用网络或者图书馆查询更多的关于动物 自我保护方式的资料,并在全班汇报调查结果。
an old coat
Discuss in groups
1. Do you know any other animals that have special ways to protect themselves?
杰奇模板详解
modules/article/templates // 文章系统模板modules/article/templates/blocks // 文章系统区块模板modules/forum/templates // 论坛系统模板modules/forum/templates/blocks // 论坛系统区块模板templates // 公用页面模板(例如用户档案)templates/blocks // 公用页面区块模板themes/default // 主要风格模板modules/article/templates/index.html // 目录页面modules/article/templates/style.html // 章节内容modules/article/templates/fulltext.html // 全文阅读modules/article/templates/articleinfo.html // 书籍摘要页面themes/default/theme.html // 动态页面公用模板模板标签下面对 theme.html 模板中使用到的标签做简单的说明:在模板文件的html源代码中插入如下标签,即可在页面中显示出来相应内容。
说明:V1.30以下版本,标签格式为<{XXX}>,V1.30以上版本标签的格式为{?XXX?},注意使用。
<{$jieqi_pagetitle}> // 网页标题<{$jieqi_charset}> // 网页字符集,对应后台系统定义中的“默认语言”<{$meta_keywords}> // 网页关键字,对应后台系统定义中的“网站关键字”<{$meta_description}> // 网站描述,对应后台系统定义中的“网站描述”<{$meta_author}> // 作者<{$meta_copyright}> // 版权申明,对应后台系统定义中的“版权申明”<{$jieqi_css}> // 系统CSS引用,由系统自动生成<{$jieqi_head}> // 头部插入代码,对应后台系统定义中的“头部插入代码”<{$jieqi_top_bar}> // 顶部通栏代码,对应后台系统定义中的“顶部通栏代码”<{$jieqi_logininfo}> // 注册用户登入详细资料<{$url_gb2312}> // GB2313编码的链接<{$url_big5}> // BIG5编码的链接<{$jieqi_imageurl}> // JIEQI CMS图片文件夹路径<{$jieqi_url}> // JIEQI CMS首页链接,对应后台系统定义中的“网站地址”<{$jieqi_banner}> // BANNER代码,对应后台系统定义中的“BANNER 代码”<{$jieqi_email}> // 联系Email,对应后台系统定义中的“联系Email”<{$jieqi_menu}> // 系统菜单,如果后台系统定义中“是否使用系统菜单”一项为是,则使用由 configs/mainmenu.php 定义的菜单项目<{$jieqi_sitename}> // 网站名称,对应后台系统定义中的“网站名称”<{$jieqi_bottom_bar}> // 底部通栏代码,对应后台系统定义中的“底部部通栏代码”<{$jieqi_tblocks[i].title}> // 顶部区块标题<{$jieqi_tblocks[i].content}> // 顶部区块内容<{$jieqi_lblocks[i].title}> // 左边区块标题<{$jieqi_lblocks[i].content}> // 左边区块内容<{$jieqi_ctblocks[i].title}> // 中上区块标题<{$jieqi_ctblocks[i].content}> // 中上区块内容<{$jieqi_clblocks[i].title}> // 中左区块标题<{$jieqi_clblocks[i].content}> // 中左区块内容<{$jieqi_crblocks[i].title}> // 中右区块标题<{$jieqi_crblocks[i].content}> // 中右区块内容<{$jieqi_cmblocks[i].title}> // 中中区块标题<{$jieqi_cmblocks[i].content}> // 中中区块内容<{$jieqi_cbblocks[i].title}> // 中下区块标题<{$jieqi_cbblocks[i].content}> // 中下区块内容<{$jieqi_rblocks[i].title}> // 右边区块标题<{$jieqi_rblocks[i].content}> // 右边区块内容<{$jieqi_bblocks[i].title}> // 底部区块标题<{$jieqi_bblocks[i].content}> // 底部区块内容为使更改后的模板生效,我们需要对相应的区块经行刷新或者清理区块缓存。
TPO12听力解析
2 本文章由尚友原创或整理
解析:1m 49 左右 细节题。“they were amazed, we all were,”此句为重点句,大家都对”只有 20%-30%的 DNA 是存在有意义基因”这个结果感到惊讶。其余选项都比较扯淡,无视之。
8. What does the professor say about the DNA in a telomere? A. It causes a cell to begin dividing. B. It separates one gene from another. C. It is genetically meaningless. D. It has no function. 答案:C 解析:2m40s 细节题。教授在 telomere(n.端粒)的定义的下句中指出:“Now a telomere is a highly repetitious and genetically meaningless sequence of DNA, what we were calling JUNK DNA.”其余选项几 乎无关。
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As with web pages, you can write HttpModules to operate asynchronously. Since they run for every request, it’s particularly important for scalability to use async operations if your HttpModule does any I/O, including database requests. The following are possible applications of HttpModules: • • • • • • Enforcing site-wide cookie policies Centralized monitoring and logging Programmatically setting or removing HTTP headers Wiring up post-processing of response output, such as removing extra whitespace Session management Authorization and authentication
■ Note Cassini, the development web server that’s used by Visual Studio, sends all requests through all HttpModules, even when the site is configured in Classic mode.
230
CHAPTER 7 ■ MANAGING APPLICATION POLICIES
Here’s a list of the requirements for the example: • • • • • Allow the default theme to be modified at runtime. Set a long-lasting cookie on each client that contains a unique ID, and record the value of that ID in the database. If the Page or the HttpModule itself sets a cookie, make sure that the P3P privacy header is also set (see Chapter 3). Differentiate between clients that retain cookies and those that don’t (as a highlevel indicator of whether they might be spider and not a real user). For all .aspx requests, log the client’s unique ID and details about the page request in the database, using a background worker thread.
Init() Method
Create MainModule.cs in your App_Code folder: using using using using using using using using using System; System.Collections.Generic; System.Data; System.Data.SqlClient; System.Diagnostics; System.Text; System.Threading; System.Web; System.Web.UI;
Requirements for the Example HttpModule
I covered a simple example of an HttpModule in Chapter 4. However, since HttpModules can play a very important role in establishing site-wide consistency and because of their potential impact on performance, let’s walk through a much more detailed example that includes some of the techniques that I’ve discussed.
CHAPTER 7 ■■■
Managing Application Policies
During the design and coding of your web site, you will often come up with actions that you would like to take for every page on your site, or perhaps for every control of a certain type. I call those actions application policies. You might apply them before or after a page generates its content or as part of page or control processing. Application policies can have a big impact on performance in areas such as session management, caching, URL rewriting, output filtering, and control rendering. They also form an important part of the ultra-fast approach, since they allow you to easily centralize, manage, and monitor certain aspects of your application. That helps improve agility while minimizing code duplication and simplifying many system-wide debugging and analysis tasks. In this chapter, I will cover the following: • • • • • • • • Using HttpModules, which allow you to apply policies at any point in the IIS request life cycle Using HttpHandlers, which allow you to bypass the policies and associated overhead imposed by the Page handler Implementing a page base class, which will allow you to override Page policies and to add others of your own Implementing URL rewriting programmatically to help shorten your URLs Using page adapters, tag mapping, and control adapters to implement application policies Using HTTP redirects and their alternatives Improving your user’s experience by flushing the response buffer early Reducing the size of your page with HTML whitespace filtering
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CHAPTER 7 ■ MANAGING APPLICATION POLICIES
Custom HttpModules
requests have a life cycle that starts with an incoming HTTP request. IIS and the runtime then step through a number of states, ultimately producing the HTTP response. At each state transition, IIS and the runtime invoke all registered event handlers. Depending on the event, those event handlers might be located in your Global.asax file or in a standard or custom HttpModule. When your site is running in IIS Integrated mode, as I suggest in Chapter 4, the pipeline events are as shown in Figure 4-2. The events happen for both static and dynamic content, and you can write event handlers in either managed or unmanaged code. For example, you could write an HttpModule in C# that handles events for your static images or even for files processed by a nonmanaged handler, such as PHP. One of the differences between Global.asax and HttpModules is that events in Global.asax are executed only for requests that are processed by the handler, even when IIS is running in Integrated mode. From an architectural perspective, I tend to discourage use of Global.asax as much as possible, since integrating a new HttpModule from another project into your site is much easier than merging multiple Global.asax files together. However, a couple of events are available only in Global.asax, and not in HttpModules, such as Application_Start and Application_Stop.