Springer-Verlag An Evolutionary Approach to Case Adaptation
微塑料/纳米塑料对维管植物的影响
1 000 nm。微塑料的外形多为圆柱形、圆形和圆盘状,颜色通
常为透明、白色和灰色,而且微塑料种类繁多,根据材质划分,主
要有聚乙烯(PE)、聚丙烯(PP)、聚苯乙烯(PS)、聚氯乙烯
每使用 5 mL 的化妆品就会导致 4 594 ~ 94 500 个球类微塑料
对智利 31 个接受不同年限污泥施用的农用土壤的调查研
究。 结果显示,随着污泥施用量的增加,土壤中微塑料的含
量也逐渐增加。 在施用了 5 次污泥后(总量为200 t / hm2 ),土
壤中微塑料的平均丰度达到了 3 500 个 / kg。
植物摄入并影响其生长发育[18-20] 。 此外,MPs / NPs 由于比
子表面,以致减少了种子对水分和养分的吸收,进而影响种
子发芽[33-34] 。 当蕨类植物( Ceratopteris pteridoides) 暴露于聚
苯乙烯纳米塑料(PS-NPs) 后,会破坏孢子从吸胀到萌发到
配子体的整个发育阶段,从而影响其繁殖过程[34] 。 吴佳妮
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安徽农业科学,J.Anhui Agric.Sci. 2023,51(20) :15-18
微塑料 / 纳米塑料对维管植物的影响
杨满香
( 重庆师范大学生命科学学院,重庆 401331)
摘要 微塑料 / 纳米塑料(micplastics / nanoplastics,MPs / NPs)广泛存在于环境中,被认为是一类对环境构成巨大威胁的新兴污染物。 通
境构成巨大威胁的新兴污染物[29] 。 基于微塑料来源广泛、
作者简介 杨满香(1997—) ,女,贵州遵义人,硕士研究生,研究方向:
新疆巩乃斯河枯、丰水期大型底栖动物群落结构与环境因子的关系
第41卷第5期生态科学41(5): 208–218 2022年9月Ecological Science Sep. 2022 王燕妮, 田伊林, 刘雨薇, 等. 新疆巩乃斯河枯、丰水期大型底栖动物群落结构与环境因子的关系[J]. 生态科学, 2022, 41(5): 208–218.WANG Yanni, TIAN Yilin, LIU Yuwei, et al. The relationship between macrobenthic community structure and environmental factors during dry and wet seasons in the Gongnaisi River, Xinjiang[J]. Ecological Science, 2022, 41(5): 208–218.新疆巩乃斯河枯、丰水期大型底栖动物群落结构与环境因子的关系王燕妮1,2, 田伊林1,2, 刘雨薇1,2, 崔东2, 尚天翠2, 姚付龙2, 张振兴1, 杨海军1,2,3,*1. 东北师范大学植被生态科学教育部重点实验室, 长春 1300242. 伊犁师范大学生物与地理科学学院, 伊宁 8350003. 云南大学生态与环境学院, 昆明 650091【摘要】为探究新疆巩乃斯河的生态状况, 团队先后在2018年10月(枯水期)和2019年6月(丰水期)对大型底栖动物群落和环境因子进行了调查, 分析大型底栖动物群落结构、功能摄食类群、生活类型组成及其与环境因子的关系。
研究河段共采集到大型底栖动物40种, 隶属3门4纲8目27科, 主要以节肢动物门为主, 其中直突摇蚊亚科(Orthocladiinae spp.)、长跗摇蚊族(Tanytansini sp.)、四节蜉属(Baeits sp.)、亚美蜉属(Ameletus sp.)和Cheilotrichia sp.是优势类群。
第六章 岛屿生物地理学理论与生物多样性保护
陆地植物
Galapagos 群岛
0 325 Preston(1962)
北方森林中的鸟
美国
0 165 Brown(1978)
北方森林中的哺乳动物
美国
0 326 Brown(1978)
浮游动物
美国纽约州湖泊
0 170 Browne(1981)
蜗牛
美国纽约州湖泊
0 230 Browne(1981)
4 库萨伊岛 Kusaie 5 图木图群岛 Tuamotu 6 马贵斯群岛 Marquesas 7 法属社会群
岛 Society lslands 8 波纳佩岛 Ponape 9 马里亚纳群岛 Marianas Islands 10 汤加岛
Tanga 11 加罗林群岛 Caroline Iqlands 12 卑硫群岛 Pelew Palau Islands 13 圣克
计算时所取单位为平方英里
动物或植物
岛屿
Байду номын сангаасz 值
来源
甲虫
西印度群岛
0 340 Darlingion(1943)
蚁类
美拉尼西亚群岛
0 300 Wilson(1992)
两栖和爬行动物
西印度群岛
0 301 Preston(1962)
繁殖的陆地和淡水鸟
西印度群岛
0 237 Hamilton 等 1964
斯群岛 New Hebrides 23 布鲁 Buru 24 希兰岛 Ceram 25 索罗门群岛 solomons
如果这一关系用一曲线表示 我们就可得到生态学中的所谓 物种 面积曲线
Species-Area curve 图 2
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The Journal of Derivatives Spring 2018, 25 (3) 7-32海外文献推荐-第四十二期参考文献:[1] Robert Capone, Adam Akant, (2016), Trend Following Strategies in Target-Date Funds, AQR Capital Management.[2] Loh, R. K., & Stulz, R. M. (2018). Is sell‐side research more valuable in bad times?. Journal of Finance, 73(3): 959-1013.海外文献推荐-第四十三期参考文献:[1] Asness, C. S., Frazzini, A., Israel, R., & Moskowitz, T. J. (2015). Fact, fiction, and value investing. Final version published in Journal of Portfolio Management, V ol. 42, No.1[2] Gu, S., Kelly, B. T., & Xiu, D. (2018). Empirical asset pricing via machine learning. Social Science Electronic Publishing.海外文献推荐-第四十四期参考文献:[1] David P. Morton, Elmira Popova, Ivilina Popova, Journal of Banking & Finance 30 (2006) 503–518海外文献推荐-第四十五期参考文献:[1] Lleo, S., & Ziemba, W. T. (2017). A tale of two indexes: predicting equity market downturns in china. Social Science Electronic Publishing海外文献推荐-第四十六期参考文献:[1] Alquist, R., Israel, R., & Moskowitz, T. J. (2018). Fact, fiction, and the size effect. Social Science Electronic Publishing.[2] Kacperczyk M, NIEUWERBURGH S V A N, Veldkamp L. Time-varying fund manager skill[J]. The Journal of Finance, 2014, 69(4): 1455-1484.海外文献推荐-第四十七期参考文献:[1] Tom Idzorek, 2008, Lifetime Asset Allocations: Methodologies for Target Maturity Funds, Ibbotson Associates Research Paper,29-47[2] Da, Z., Huang, D., & Yun, H. (2017). Industrial electricity usage and stock returns. Journal of Financial & Quantitative Analysis, 52(1), 37-69.海外文献推荐-第四十八期参考文献:[1] Clifford Asness and Andrea Frazzini, 2013, The Devil in HML’s Details, The Journal of Portfolio Management, volume 39 number 4.[2] Carvalho, R. L. D., Xiao, L., & Moulin, P. (2011). Demystifying equity risk-based strategies: a simple alpha plus beta description.Journal of Portfolio Management,38(3), 56-70.海外文献推荐-第四十九期参考文献:[1]Jordan Brooks, Diogo Palhares, Scott Richardson, Style investing in fixed income, Journal of Portfolio Management.[2] R Ball,J Gerakos,JT Linnainmaa,V Nikolaev,2015,Deflating profitability,Journal of Financial Economics, 117 (2) :225-248海外文献推荐-第五十期参考文献:[1] Padmakar Kulkarni, Abhishek Gupta, Stuart Doole, 2018, How can Factors be Combined, MSCI.[2] Hsieh, C. C., Hui, K. W., & Zhang, Y. (2016). Analyst report readability and stock returns. Journal of Business Finance & Accounting, 43(1-2), págs. 98-130.海外文献推荐-第五十一期参考文献:[1] Cici G, Rosenfeld C. A study of analyst-run mutual funds: The abilities and roles of buy-side analysts [J]. Journal of Empirical Finance, 2016, 36:8-29.[2] U-Wen Kok, CFA, Jason Ribando, CFA, and Richard Sloan Facts about Formulaic Value Investing Financial Analysts Journal. V olume 73, Issue 2海外文献推荐-第五十二期参考文献:[1] Morningstar Manager Research.(2018)Target-Date Fund Landscape. 7 May 2018[2] Yong Chen, Gregory W. Eaton, Bradley S. Paye, Micro(structure) before Macro? The Predictive Power of Aggregate llliquidity for Stock Returns and Economic Activity, Journal of Financial Economics (2018), doi: 10.1016/j.jfineco.2018.05.011海外文献推荐-第五十三期参考文献:[1]Arnott R D, Chaves D B, Chow T. King of the Mountain:, Shiller P/E and Macroeconomic Conditions[J]. Social Science Electronic Publishing, 2015, 44(1):55-68.[2]Risk Parity Portfolio vs. Other Asset Allocation Heuristic Portfolios [J]. The Journal of Investing. 2010 December海外文献推荐-第五十四期参考文献:[1]Cliff's Perspective, Our Model Goes to Six and Saves Value From Redundancy Along the Way,AQR Capital Management, December 17, 2014[2]D Avramov,S Cheng,A Schreiber,K Shemer,2017,Scaling up Market Anomalies,Social Science Electronic Publishing,26 (3) :89-105海外文献推荐-第五十五期参考文献:[1]Aurélien Philippot,Analysts’ reinitiations of coverage and market underreaction,Journal of Banking and Finance , 94 (2018) 208–220海外文献推荐-第五十六期参考文献:[1]Michael W. Brandt, Earnings Announcements are Full of Surprises,Social Science Electronic Publishing, January 22, 2008[2]Sujin Pyo, Jaewook Lee,Exploiting the low-risk anomaly using machine learning to enhance the Black–Litterman framework: Evidence from South Korea,Pacific-Basin Finance Journal,51 (2018) 1–12[3]Robert F Engle and Andrew J Patton,What good is a volatility model?,Robert F Engle and Andrew J Patton海外文献推荐-第五十七期参考文献:[1]Nic Schaub, The Role of Data Providers as Information Intermediaries,Social Science Electronic Publishing, 2015 :1-34海外文献推荐-第五十八期参考文献:[1]Binu George and Hardik Shah, ESG: Improving Your Risk-Adjusted Returns in Emerging Markets,GMO White Paper, Mar 2018海外文献推荐-第五十九期参考文献:[1]Campbell R. Harvey and Yan Liu. Backtesting. Journal of portfolio management, 2015海外文献推荐-第六十期参考文献:[1]Mclean R D, Pontiff J. Does Academic Research Destroy Stock Return Predictability?[J]. Journal of Finance, 2016, 71(1)海外文献推荐-第六十一期参考文献:[1]Israelov R, Tummala H. Which Index Options Should You Sell?[J]. Social Science Electronic Publishing, 2017海外文献推荐-第六十二期参考文献:[1]Eric H. Sorensen, Keith L. Miller, and Chee K. Ooi,2000,The Decision Tree Approach to Stock Selection,The Journal of Portfolio Management,42-52海外文献推荐-第六十三期参考文献:[1]Donangelo A, Gourio F, Kehrig M, et al. The cross-section of labor leverage and equity returns[J]. Journal of Financial Economics, 2018海外文献推荐-第六十四期参考文献:[1]Qang Bu. Do Persistent Fund Alphas Indicate Manager Skill? [J]. Journal of Wealth Management,2017,20(2)82-93海外文献推荐-第六十五期参考文献:[1]Miguel A. Lejeune A VaR Black–Litterman model for the construction of absolute return fund-offunds [J] Quantitative Finance · January 2009海外文献推荐-第六十六期参考文献:[1]Fan J H, Zhang T. Demystifying Commodity Futures in China [J]. Social Science Electronic Publishing, 2018海外文献推荐-第六十七期参考文献:[1]Jon Hale, Sustainable Funds U.S. Landscape Report. Morningstar Research, 2018.海外文献推荐-第六十八期参考文献:[1]Sun Z, Wang A, Zheng L. Only Winners in Tough Times Repeat: Hedge Fund Performance Persistence over Different Market Conditions[J]. Journal of Financial and Quantitative Analysis, 2018.海外文献推荐-第六十九期参考文献:[1] A´LVARO CARTEA,SEBASTIAN JAIMUNGAL. RISK METRICS AND FINE TUNING OF HIGH-FREQUENCY TRADING STRATEGIES [J]. Mathematical Finance, V ol. 00, No. 0 (xxx 2013), 1-36.海外文献推荐-第七十期参考文献:[1] Dopfel, Frederick E. , and L. Ashley . "Optimal Blending of Smart Beta and Multifactor Portfolios." The Journal of Portfolio Management 44.4(2018):93-105.海外文献推荐-第七十一期参考文献:[1] Avraham Kamara, Robert Korajczyk, Xiaoxia Lou and Ronnie Sadka,2018,Short-Horizon Beta or Long-Horizon Alpha?, The Journal of Portfolio Management,45(1),96-105海外文献推荐-第七十二期参考文献:[1] Masulis, Ronald W., and Emma Jincheng Zhang. "How valuable are independent directors? Evidence from external distractions." Journal of Financial Economics (2018).海外文献推荐-第七十三期参考文献:[1] Hunter D, Kandel E, Kandel S, et al. Mutual fund performance evaluation with active peer benchmarks[J]. Journal of Financial economics, 2014, 112(1): 1-29.海外文献推荐-第七十四期参考文献:[1]Michael Stein and Svetlozar T. Rachev. Style Neutral Funds of Funds: Diversification or Deadweight? [J]. Journal of Asset Management, February 2011, V olume 11, Issue 6, pp 417–434海外文献推荐-第七十五期参考文献:[1] Elisabeth Kashner, 2019.01.31, Bogle led this investing Fee War, ;[2] Cinthia Murphy,2017,03.31, how to launch a successful ETF, ;[3] Drew V oros, 2019.01.23, how a small ETF Issuer Competes, ;[4] 2019.01.04, Invesco focusing on scale,海外文献推荐-第七十六期参考文献:[1] Shpak I , Human B , Nardon A . Idiosyncratic momentum in commodity futures[J]. Social Science Electronic Publishing, 2017.海外文献推荐-第七十六期参考文献:[1] Ehsani S , Linnainmaa J T . Factor Momentum and the Momentum Factor[J]. Social Science Electronic Publishing, 2017.海外文献推荐-第七十七期参考文献:[1] Iuliia Shpak*, Ben Human and Andrea Nardon. 2017.09.11, Idiosyncratic momentum in commodity futures. ResearchGate海外文献推荐-第七十八期参考文献:[1] Joel Hasbrouck. High-Frequency Quoting: Short-Term V olatility in Bids and Offers. JOURNAL OF FINANCIAL AND QUANTITATIVE ANALYSIS海外文献推荐-第七十九期参考文献:[1] Tarun Gupta and Bryan Kelly. Factor Momentum Everywhere. Institutional Investor Journals海外文献推荐-第八十期参考文献:[1] MICHAEL A. BABYAK , P H D. What You See May Not Be What You Get: A Brief, Nontechnical Introduction to Overfitting in Regression-Type Models. S T A T I S T I C A L C O R N E R海外文献推荐-第八十一期参考文献:[1] Eric Jondeau , Qunzi Zhang , Xiaoneng Zhu. Average Skewness Matters.海外文献推荐-第八十二期参考文献:[1] JOHN A. HASLEM. Morningstar Mutual Fund Measures and Selection Model. THE JOURNAL OF WEALTH MANAGEMENT海外文献推荐-第八十三期参考文献:[1] EUGENE F. FAMA and KENNETH R. FRENCH. Luck versus Skill in the Cross-Section of Mutual Fund Returns. THE JOURNAL OF FINANCE海外文献推荐-第八十四期参考文献:[1] How Transparent Are ETFs?[2] Lara Crigger. Nontransparent Active: Next ETF Revolution?.海外文献推荐-第八十五期参考文献:[1] Olivier Rousse and Benoît Sévi. Informed Trading in Oil-Futures Market. Fondazione Eni Enrico Mattei (FEEM)海外文献推荐-第八十六期参考文献:[1] Ari Levine and Lasse Heje Pedersen. Which Trend is Your Friend?。
软件工程英文参考文献(优秀范文105个)
当前,计算机技术与网络技术得到了较快发展,计算机软件工程进入到社会各个领域当中,使很多操作实现了自动化,得到了人们的普遍欢迎,解放了大量的人力.为了适应时代的发展,社会各个领域大力引进计算机软件工程.下面是软件工程英文参考文献105个,供大家参考阅读。
软件工程英文参考文献一:[1]Carine Khalil,Sabine Khalil. Exploring knowledge management in agile software development organizations[J]. International Entrepreneurship and Management Journal,2020,16(4).[2]Kevin A. Gary,Ruben Acuna,Alexandra Mehlhase,Robert Heinrichs,Sohum Sohoni. SCALING TO MEET THE ONLINE DEMAND IN SOFTWARE ENGINEERING[J]. International Journal on Innovations in Online Education,2020,4(1).[3]Hosseini Hadi,Zirakjou Abbas,Goodarzi Vahabodin,Mousavi Seyyed Mohammad,Khonakdar Hossein Ali,Zamanlui Soheila. Lightweight aerogels based on bacterial cellulose/silver nanoparticles/polyaniline with tuning morphology of polyaniline and application in soft tissue engineering.[J]. International journal of biological macromolecules,2020,152.[4]Dylan G. Kelly,Patrick Seeling. Introducing underrepresented high school students to software engineering: Using the micro:bit microcontroller to program connected autonomous cars[J]. Computer Applications in Engineering Education,2020,28(3).[5]. Soft Computing; Research Conducted at School of Computing Science and Engineering Has Updated Our Knowledge about Soft Computing (Indeterminate Likert scale: feedback based on neutrosophy, its distance measures and clustering algorithm)[J]. News of Science,2020.[6]. Engineering; New Engineering Findings from Hanyang University Outlined (Can-based Aging Monitoring Technique for Automotive Asics With Efficient Soft Error Resilience)[J]. Journal of Transportation,2020.[7]. Engineering - Software Engineering; New Findings from University of Michigan in the Area of Software Engineering Reported (Multi-criteria Test Cases Selection for Model Transformations)[J]. Journal of Transportation,2020.[8]Tamas Galli,Francisco Chiclana,Francois Siewe. Software Product Quality Models, Developments, Trends, and Evaluation[J]. SN Computer Science,2020,1(2).[9]. Infotech; Infotech Joins BIM for Bridges and Structures Transportation Pooled Fund Project as an Official Software Advisor[J]. Computer TechnologyJournal,2020.[10]. Engineering; Study Findings from Beijing Jiaotong University Provide New Insights into Engineering (Analyzing Software Rejuvenation Techniques In a Virtualized System: Service Provider and User Views)[J]. Computer Technology Journal,2020.[11]. Soft Computing; Data on Soft Computing Reported by Researchers at Sakarya University (An exponential jerk system, its fractional-order form with dynamical analysis and engineering application)[J]. Computer Technology Journal,2020.[12]. Engineering; Studies from Henan University Yield New Data on Engineering (Extracting Phrases As Software Features From Overlapping Sentence Clusters In Product Descriptions)[J]. Computer Technology Journal,2020.[13]. Engineering; Data from Nanjing University of Aeronautics and Astronautics Provide New Insights into Engineering (A Systematic Study to Improve the Requirements Engineering Process in the Domain of Global Software Development)[J]. Computer Technology Journal,2020.[14]. Soft Computing; Investigators at Air Force Engineering University Report Findings in Soft Computing (Evidential model for intuitionistic fuzzy multi-attribute group decision making)[J]. Computer Technology Journal,2020.[15]. Engineering; Researchers from COMSATS University Islamabad Describe Findings in Engineering (A Deep CNN Ensemble Framework for Efficient DDoS Attack Detection in Software Defined Networks)[J]. Computer Technology Journal,2020.[16]Pedro Delgado-Pérez,Francisco Chicano. An Experimental and Practical Study on the Equivalent Mutant Connection: An Evolutionary Approach[J]. Information and Software Technology,2020.[17]Koehler Leman Julia,Weitzner Brian D,Renfrew P Douglas,Lewis Steven M,Moretti Rocco,Watkins Andrew M,Mulligan Vikram Khipple,Lyskov Sergey,Adolf-Bryfogle Jared,Labonte Jason W,Krys Justyna,Bystroff Christopher,Schief William,Gront Dominik,Schueler-Furman Ora,Baker David,Bradley Philip,Dunbrack Roland,Kortemme Tanja,Leaver-Fay Andrew,Strauss Charlie E M,Meiler Jens,Kuhlman Brian,Gray Jeffrey J,Bonneau Richard. Better together: Elements of successful scientific software development in a distributed collaborative community.[J]. PLoS computational biology,2020,16(5).[18]. Mathematics; Data on Mathematics Reported by Researchers at Thapar Institute of Engineering and Technology (Algorithms Based on COPRAS and Aggregation Operators with New Information Measures for Possibility Intuitionistic Fuzzy SoftDecision-Making)[J]. Journal of Mathematics,2020.[19]. Engineering - Medical and Biological Engineering; Reports from Heriot-Watt University Describe Recent Advances in Medical and Biological Engineering (A Novel Palpation-based Method for Tumor Nodule Quantification In Soft Tissue-computational Framework and Experimental Validation)[J]. Journal of Engineering,2020.[20]. Engineering - Industrial Engineering; Studies from Xi'an Jiaotong University Have Provided New Data on Industrial Engineering (Dc Voltage Control Strategy of Three-terminal Medium-voltage Power Electronic Transformer-based Soft Normally Open Points)[J]. Journal of Engineering,2020.[21]. Engineering; Reports from Hohai University Add New Data to Findings in Engineering (Soft Error Resilience of Deep Residual Networks for Object Recognition)[J]. Journal of Engineering,2020.[22]. Engineering - Mechanical Engineering; Study Data from K.N. Toosi University of Technology Update Understanding of Mechanical Engineering (Coupled Directional Dilation-Damage Approach to Model the Cyclic-Undrained Response of Soft Clay under Pure Principal Stress Axes Rotation)[J]. Journal of Engineering,2020.[23]. Soft Computing; Researchers from Abes Engineering College Report Details of New Studies and Findings in the Area of Soft Computing (An intelligent personalized web blog searching technique using fuzzy-based feedback recurrent neural network)[J]. Network Weekly News,2020.[24]. Engineering; Studies from University of Alexandria in the Area of Engineering Reported (Software Defined Network-Based Management for Enhanced 5G Network Services)[J]. Network Weekly News,2020.[25]. Soft Computing; Data on Soft Computing Discussed by Researchers at Department of Electrical and Communication Engineering [A metaheuristic optimization model for spectral allocation in cognitive networks based on ant colony algorithm (M-ACO)][J]. Computer Technology Journal,2020.[26]. Engineering - Software Engineering; Complutense University Madrid Reports Findings in Software Engineering (Recolibry Suite: a Set of Intelligent Tools for the Development of Recommender Systems)[J]. Computer Technology Journal,2020.[27]. Engineering - Software Engineering; Data on Software Engineering Reported by Researchers at Gautam Buddha University (A novel quality prediction model for component based software system using ACO-NM optimized extreme learning machine)[J]. Computer Technology Journal,2020.[28]. Soft Computing; New Soft Computing Study Findings Recently Were Reported by Researchers at University College of Engineering (A novel QIM-DCT based fusion approach for classification of remote sensing images via PSO and SVM models)[J]. Computer Technology Journal,2020.[29]Morshedloo Fatemeh,Khoshfetrat Ali Baradar,Kazemi Davoud,Ahmadian Mehri. Gelatin improves peroxidase-mediated alginate hydrogel characteristics as a potential injectable hydrogel for soft tissue engineering applications.[J]. Journal of biomedical materials research. Part B, Applied biomaterials,2020.[30]Jung-Chieh Lee,Chung-Yang Chen. Exploring the team dynamic learning process in software process tailoring performance[J]. Journal of Enterprise Information Management,2020,33(3).[31]. Soft Computing; Study Results from Velammal Engineering College in the Area of Soft Computing Reported (Efficient routing in UASN during the thermohaline environment condition to improve the propagation delay and throughput)[J]. Mathematics Week,2020.[32]. Soft Matter; Findings from School of Materials Science and Engineering Provide New Insights into Soft Matter (A practical guide to active colloids: choosing synthetic model systems for soft matter physics research)[J]. Physics Week,2020.[33]Julio César Puche-Regaliza,Alfredo Jiménez,Pablo Arranz-Val. Diagnosis of Software Projects Based on the Viable System Model[J]. Systemic Practice and Action Research,2020,33(1).[34]Meinert Edward,Milne-Ives Madison,Surodina Svitlana,Lam Ching. Agile requirements engineering and software planning for a digital health platform to engage the effects of isolation caused by social distancing: A case study and feasibility study protocol.[J]. JMIR public health and surveillance,2020.[35]. Engineering - Civil Engineering; Studies Conducted at Shandong Jianzhu University on Civil Engineering Recently Published (Seismic Response Analysis and Control of Frame Structures with Soft First Storey under Near-Fault Ground Motions)[J]. Journal of Engineering,2020.软件工程英文参考文献二:[36]Chao-ze Lu,Guo-sun Zeng,Ying-jie Xie. Bigraph specification of software architecture and evolution analysis in mobile computing environment[J]. Future Generation Computer Systems,2020,108.[37]Ompal Singh, Saurabh Panwar, P. K. Kapur.. Determining SoftwareTime-to-Market and Testing Stop Time when Release Time is a Change-Point[J]. International Journal of Mathematical, Engineering and Management Sciences,2020,5(2).[38]Ayushi Verma,Neetu Sardana,Sangeeta Lal. Developer Recommendation for Stack Exchange Software Engineering Q&A Website based on K-Means clustering and Developer Social Network Metric[J]. Procedia Computer Science,2020,167.[39]Jagdeep Singh,Sachin Bagga,Ranjodh Kaur. Software-based Prediction of Liver Disease with Feature Selection and Classification Techniques[J]. Procedia Computer Science,2020,167.[40]. Engineering - Software Engineering; Studies from Concordia University Update Current Data on Software Engineering (On the impact of using trivial packages: an empirical case study on npm and PyPI)[J]. Computer Technology Journal,2020.[41]. Engineering - Software Engineering; Study Findings from University of Alberta Broaden Understanding of Software Engineering (Building the perfect game - an empirical study of game modifications)[J]. Computer Technology Journal,2020.[42]. Engineering - Software Engineering; Investigators at National Research Council (CNR) Detail Findings in Software Engineering [A Framework for Quantitative Modeling and Analysis of Highly (Re)Configurable Systems][J]. Computer Technology Journal,2020.[43]. Engineering - Knowledge Engineering; Data from University of Paris Saclay Provide New Insights into Knowledge Engineering (Dynamic monitoring of software use with recurrent neural networks)[J]. Computer Technology Journal,2020.[44]. Engineering - Circuits Research; Findings from Federal University Santa Maria Yields New Data on Circuits Research (A New Cpfsk Demodulation Approach for Software Defined Radio)[J]. Computer Technology Journal,2020.[45]. Soft Computing; Investigators from Lovely Professional University Release New Data on Soft Computing (An intensify Harris Hawks optimizer for numerical and engineering optimization problems)[J]. Computer Technology Journal,2020.[46]. GlobalLogic Inc.; GlobalLogic Acquires Meelogic Consulting AG, a European Healthcare and Automotive-Focused Software Engineering Services Firm[J]. Computer Technology Journal,2020.[47]. Engineering - Circuits and Systems Research; Data on Circuits and Systems Research Described by Researchers at Northeastern University (Softcharge: Software Defined Multi-device Wireless Charging Over Large Surfaces)[J]. TelecommunicationsWeekly,2020.[48]. Soft Computing; Researchers from Department of Electrical and Communication Engineering Report on Findings in Soft Computing (Dynamic Histogram Equalization for contrast enhancement for digital images)[J]. Technology News Focus,2020.[49]Mohamed Ellithey Barghoth,Akram Salah,Manal A. Ismail. A Comprehensive Software Project Management Framework[J]. Journal of Computer and Communications,2020,08(03).[50]. Soft Computing; Researchers from Air Force Engineering University Describe Findings in Soft Computing (Random orthocenter strategy in interior search algorithm and its engineering application)[J]. Journal of Mathematics,2020.[51]. Soft Computing; Study Findings on Soft Computing Are Outlined in Reports from Department of Mechanical Engineering (Constrained design optimization of selected mechanical system components using Rao algorithms)[J]. Mathematics Week,2020.[52]Iqbal Javed,Ahmad Rodina B,Khan Muzafar,Fazal-E-Amin,Alyahya Sultan,Nizam Nasir Mohd Hairul,Akhunzada Adnan,Shoaib Muhammad. Requirements engineering issues causing software development outsourcing failure.[J]. PloS one,2020,15(4).[53]Raymond C.Z. Cohen,Simon M. Harrison,Paul W. Cleary. Dive Mechanic: Bringing 3D virtual experimentation using biomechanical modelling to elite level diving with the Workspace workflow engine[J]. Mathematics and Computers in Simulation,2020,175.[54]Emelie Engstr?m,Margaret-Anne Storey,Per Runeson,Martin H?st,Maria Teresa Baldassarre. How software engineering research aligns with design science: a review[J]. Empirical Software Engineering,2020(prepublish).[55]Christian Lettner,Michael Moser,Josef Pichler. An integrated approach for power transformer modeling and manufacturing[J]. Procedia Manufacturing,2020,42.[56]. Engineering - Mechanical Engineering; New Findings from Leibniz University Hannover Update Understanding of Mechanical Engineering (A finite element for soft tissue deformation based on the absolute nodal coordinate formulation)[J]. Computer Technology Journal,2020.[57]. Science - Social Science; Studies from University of Burgos Yield New Information about Social Science (Diagnosis of Software Projects Based on the Viable System Model)[J]. Computer Technology Journal,2020.[58]. Technology - Powder Technology; Investigators at Research Center Pharmaceutical Engineering GmbH Discuss Findings in Powder Technology [Extended Validation and Verification of Xps/avl-fire (Tm), a Computational Cfd-dem Software Platform][J]. Computer Technology Journal,2020.[59]Guadalupe-Isaura Trujillo-Tzanahua,Ulises Juárez-Martínez,Alberto-Alfonso Aguilar-Lasserre,María-Karen Cortés-Verdín,Catherine Azzaro-Pantel. Multiple software product lines to configure applications of internet of things[J]. IET Software,2020,14(2).[60]Eduardo Juárez,Rocio Aldeco-Pérez,Jose.Manuel Velázquez. Academic approach to transform organisations: one engineer at a time[J]. IET Software,2020,14(2).[61]Dennys García-López,Marco Segura-Morales,Edson Loza-Aguirre. Improving the quality and quantity of functional and non-functional requirements obtained during requirements elicitation stage for the development of e-commerce mobile applications: an alternative reference process model[J]. IET Software,2020,14(2).[62]. Guest Editorial: Software Engineering Applications to Solve Organisations Issues[J]. IET Software,2020,14(2).[63]?,?. Engine Control Unit ? ? ?[J]. ,2020,47(4).[64]. Engineering - Software Engineering; Study Data from Nanjing University Update Understanding of Software Engineering (Identifying Failure-causing Schemas In the Presence of Multiple Faults)[J]. Mathematics Week,2020.[65]. Energy - Renewable Energy; Researchers from Institute of Electrical Engineering Detail New Studies and Findings in the Area of Renewable Energy (A Local Control Strategy for Distributed Energy Fluctuation Suppression Based on Soft Open Point)[J]. Journal of Mathematics,2020.[66]Ahmed Zeraoui,Mahfoud Benzerzour,Walid Maherzi,Raid Mansi,Nor-Edine Abriak. New software for the optimization of the formulation and the treatment of dredged sediments for utilization in civil engineering[J]. Journal of Soils and Sediments,2020(prepublish).[67]. Engineering - Concurrent Engineering; Reports from Delhi Technological University Add New Data to Findings in Concurrent Engineering (Systematic literature review of sentiment analysis on Twitter using soft computing techniques)[J]. Journal of Engineering,2020.[68]. Engineering; New Findings from Future University in Egypt in the Area of Engineering Reported (Decision support system for optimum soft clay improvementtechnique for highway construction projects)[J]. Journal of Engineering,2020.[69]Erica Mour?o,Jo?o Felipe Pimentel,Leonardo Murta,Marcos Kalinowski,Emilia Mendes,Claes Wohlin. On the performance of hybrid search strategies for systematic literature reviews in software engineering[J]. Information and Software Technology,2020,123.[70]. Soft Computing; Researchers from Anna University Discuss Findings in Soft Computing (A novel fuzzy mechanism for risk assessment in software projects)[J]. News of Science,2020.软件工程英文参考文献三:[71]. Software and Systems Research; New Software and Systems Research Study Results from Chalmers University of Technology Described (Why and How To Balance Alignment and Diversity of Requirements Engineering Practices In Automotive)[J]. Journal of Transportation,2020.[72]Anupama Kaushik,Devendra Kr. Tayal,Kalpana Yadav. A Comparative Analysis on Effort Estimation for Agile and Non-agile Software Projects Using DBN-ALO[J]. Arabian Journal for Science and Engineering,2020,45(6).[73]Subhrata Das,Adarsh Anand,Mohini Agarwal,Mangey Ram. Release Time Problem Incorporating the Effect of Imperfect Debugging and Fault Generation: An Analysis for Multi-Upgraded Software System[J]. International Journal of Reliability, Quality and Safety Engineering,2020,27(02).[74]Saerom Lee,Hyunmi Baek,Sehwan Oh. 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小花山柰根状茎挥发油和营养成分及其抗植物病原菌活性分析
·2081·小花山柰根状茎挥发油和营养成分及其抗植物病原菌活性分析冯莹1,仇思润2,王张豪2,何宇豪2,黄茵茵3,李逸彤2,李婉琳2,单体江2*(1广东省森林资源保育中心,广东广州510173;2华南农业大学林学与风景园林学院,广东广州510642;3广州医科大学附属口腔医院,广东广州510182)摘要:【目的】阐明小花山柰根状茎挥发油的化学组成和相对含量,明确根状茎中多种营养成分含量,分析根状茎不同提取物的抗菌活性,从而为小花山柰资源的综合开发利用提供理论依据。
【方法】采用水蒸汽蒸馏法提取小花山柰挥发油,通过气相色谱—质谱联用仪(GC-MS )对挥发油进行化学成分分析;采用凯氏定氮法、酸—苯酚比色法和电感耦合等离子体—质谱法(ICP-MS )半定量分析法分别测定小花山柰根状茎中蛋白质、粗多糖和微量元素含量,采用茚三酮柱后衍生离子交换色谱法测定氨基酸组成与含量;进一步采用抑菌圈法和菌丝生长速率法测定小花山柰根状茎不同提取物的抗细菌和抗真菌活性。
【结果】小花山柰根状茎中挥发油得率为0.14‰;从根状茎挥发油中共鉴定出66种成分,占挥发性成分总量的94.51%,主要有烯烃类、醇类、醛类、酮类和酯类,主要成分为冰片(13.02%)、芳樟醇(12.27%)和大根香叶烯D (5.42%)等。
小花山柰根状茎中蛋白质含量为3.83g/100g ,粗多糖含量为3.33g/100g ;16种氨基酸总量为2.94g/100g ,其中精氨酸含量最高(0.61g/100g ),其次是天冬氨酸(0.46g/100g )。
小花山柰根状茎中共检测出28种微量元素,其中钾元素含量最高(3270mg/kg ),镁(813mg/kg )、钙(289mg/kg )、锰(146mg/kg )和铝(135mg/kg )的含量也较高,其他微量元素的含量均在80mg/kg 以下。
小花山柰根状茎乙酸乙酯层提取物的抗细菌活性最强,石油醚层提取物对杜英生假隐丛赤壳菌的抑制活性最强,半最大效应浓度(EC 50)为14.11μg/mL 。
青藏高原及邻区大地构造单元初步划分
记录了晚古生代 — 中生代弧后扩张、多岛弧盆系发 育、 弧# 弧碰撞、 弧#陆碰撞的地质演化历史。 碰撞之 后该区的大部分地区于晚三叠世转化为陆地,并形 成碰撞后地壳伸展背景下的裂陷或裂谷盆地。 冈瓦纳北缘晚古生代 — 中生代冈底斯 # 喜马拉 雅构造区:班公湖 # 丁青 # 碧土 # 昌宁 # 孟连对接带 是冈瓦纳与劳亚 # 泛华夏大陆的分界线,亦即冈瓦 纳大陆的北界。伯舒拉岭 # 高黎贡山属于冈瓦纳晚 古生代 — 中生代前锋弧, 聂荣隆起、 嘉玉桥变质地体 等是前锋弧的残块。在前锋弧的后面 (南侧) 是晚古 生代 — 中生代冈底斯 # 喜马拉雅弧后扩张、多岛弧 盆系发育、 弧 #弧碰撞、 弧#陆碰撞的演化结果。 该区 三叠纪和侏罗纪 — 早白垩世的雅鲁藏布江蛇绿岩是 目前青藏高原乃至中国大陆内, 保存最好、 最完整的 蛇绿岩 “三位一体” 组合, 代表了特提斯洋向南俯冲 诱导出的一系列藕断丝连的弧后扩张盆地。
(或晚古 !#*&#奥依塔格 &库地 &苏巴什 &东昆中结合带 生代裂谷带) (含前寒武纪岩块、 海山和洋岛残块) !#*&!南昆仑残余弧
#+&!高喜马拉雅结晶岩带或基底逆冲带 #+&)低喜马拉雅褶冲带 #+&+锡伐利克后造山前陆盆地带
!#*&)麻扎&康西瓦&木孜塔格&西大滩晚古生代结合带 ! 泛华夏大陆晚古生代羌塘 &三江构造区 "#扬子陆块:
青藏高原具有复杂而独特的巨厚地壳和岩石圈 结构, 是一个由北部劳亚大陆、 泛华夏陆块西缘和南 部冈瓦纳大陆北缘不断弧后扩张、裂离,又互相对 接、 镶嵌构成的复杂地区, 经历了漫长的构造变动历 史, 特别是古生代以来的多岛弧盆系的形成演化, 最 终由!"多条规模不等的弧#弧、弧#陆碰撞结合带和 其间的岛弧或陆块拼贴而成。由于后期印度板块向 北强烈顶撞,在其左右犄角处分别形成帕米尔和横 断山构造结及相应的弧形弯折,在东西两端改变了 原来东西向展布的构造面貌,加之华北和扬子刚性 陆块的阻抗和陆内俯冲对原有构造,特别是深部地 幔构造的改造, 造成了青藏高原独特的构造、 地貌景 观, 形成了统一的深部幔拗和地表的隆升。
几株拮抗水稻纹枯病菌东乡野生稻内生真菌的鉴定及活性分析
几株拮抗水稻纹枯病菌东乡野生稻内生真菌的鉴定及活性分析刘德;高波良;朱开明;邹宇炬;张志斌【摘要】为探究东乡野生稻内生真菌中生物活性菌株的种群分布和拮抗潜力,本文采用菌落形态观察及ITS-rDNA序列分析法分别对5株前期获得的具有抑制水稻纹枯病菌的内生真菌进行种属鉴定,菌株DX-THL3、DX-SER3、DX-THL2、DX-SES3和DX-THS2分属于帚枝霉(Sarocladium oryzae)、正青霉(Eupenicillium sp.)、黑孢霉(Nigrospora sphaerica)、小球壳孢属(Microsphaeropsis arundinis)和Saccharicola sp..菌丝生长抑制法测定结果显示,有4株菌株发酵浓缩液对水稻纹枯病菌有抑制活性,其中菌株DX-THL3和DX-SES3发酵培养8d对水稻纹枯病菌的抑制率最高,显示了良好的生物防治水稻纹枯病菌的潜力,该研究为东乡野生稻内生真菌资源在水稻病害防治中的应用奠定了基础.【期刊名称】《宜春学院学报》【年(卷),期】2015(037)009【总页数】5页(P6-10)【关键词】东乡野生稻;内生真菌;ITS序列;抑菌活性【作者】刘德;高波良;朱开明;邹宇炬;张志斌【作者单位】江西师范大学生命科学学院,江西省亚热带植物资源保护与利用重点实验室,南昌330022;江西师范大学生命科学学院,江西省亚热带植物资源保护与利用重点实验室,南昌330022;江西师范大学生命科学学院,江西省亚热带植物资源保护与利用重点实验室,南昌330022;江西省鄱阳县第一中学,江西鄱阳333100;东乡县农业技术推广中心,江西东乡 331800;江西师范大学生命科学学院,江西省亚热带植物资源保护与利用重点实验室,南昌330022【正文语种】中文【中图分类】S476.1水稻纹枯病是由立枯丝核菌 (Rhizotonia solani)引起的水稻重要病害之一,通过侵染水稻叶鞘和叶片引起枯斑,使水稻结实率降低,瘪谷率增加,减产最高可超过30%。
Establishment of a transgenic hairy root system in wild
ORIGINAL PAPEREstablishment of a transgenic hairy root system in wildand domesticated watermelon (Citrullus lanatus )for studying root vigor under droughtMasataka Kajikawa •Kaoru Morikawa •Yosuke Abe •Akiho Yokota •Kinya AkashiReceived:25December 2009/Revised:16April 2010/Accepted:19April 2010/Published online:6May 2010ÓSpringer-Verlag 2010Abstract Root vigor is an important trait for the growth of terrestrial plants,especially in water-deficit environ-ments.Although deserts plants are known for their highly developed root architecture,the molecular mechanism responsible for this trait has not been determined.Here we established an efficient protocol for the genetic manipula-tion of two varieties of watermelon plants:a desert-grown wild watermelon that shows vigorous root growth under drought,and a domesticated cultivar showing retardation of root growth under drought stress.Agrobacterium rhizo-genes -mediated transgenic hairy roots were efficiently induced and selected from the hypocotyls of these plants.Transgenic GUS expression was detected in the roots by RT-PCR and histochemical GUS staining.Moreover,a liquid culture system for evaluating their root growth was also established.Interestingly,growth of the hairy roots derived from domesticated variety of watermelon strongly inhibited under high osmotic condition,whereas the hairy roots derived from wild variety of watermelon retained substantial growth rates under the stress condition.The new protocol presented here offers a powerful tool for the comparative study of the molecular mechanism underlying drought-induced root growth in desert plants.Keywords Wild watermelon ÁAgrobacterium rhizogenes ÁHairy root ÁGUSIntroductionWild watermelon (Citrullus lanatus sp.),which inhabit the Kalahari Desert in Botswana,exhibit strong resistance to drought compared with domesticated watermelon cultivar (Citrullus lanatus cv.)(Kawasaki et al.2000;Takahara et al.2005;Akashi et al.2008;Kohzuma et al.2009).Wild watermelon extends its highly developed root system into deep soil layers,which contributes not only to the efficient water uptake but also during water storage (Larcher 1995).Interestingly,it was demonstrated that the onset of drought stimulated vigorous root growth in wild watermelon,whereas the same treatment contrastingly resulted in the suppression of root growth in domesticated watermelon (Yoshimura et al.2008).These observations suggested that the wild watermelon is equipped with unique molecular mechanisms that promote root development under drought conditions.Recently,our group reported a proteome analysis of wild watermelon roots under drought,which revealed that the expression of a unique set of proteins was differentially modulated in a temporally regulated manner (Yoshimura et al.2008).To understand the function of these drought-responsive proteins in detail,a comparative study between wild and domesticated watermelon via genetic manipula-tion of the gene in question will be a powerful approach.Although the Agrobacterium tumefaciens -mediated trans-formation and regeneration of wild and domesticated watermelon plants has been reported (Choi et al.1994;Ellul et al.2003;Akashi et al.2005),the protocol involves time-consuming processes to obtain the next generation of transgenic plants.Transgenic hairy root in vitro culture system induced by A.rhizogenes was proposed as an effective and rapid transformation system for studying gene function in plantCommunicated by F.Sato.M.Kajikawa ÁK.Morikawa ÁY.Abe ÁA.Yokota ÁK.Akashi (&)Nara Institute of Science and Technology,Graduate School of Biological Sciences,8916-5Takayama,Ikoma,Nara 630-0192,Japan e-mail:akashi@bs.naist.jp123Plant Cell Rep (2010)29:771–778DOI 10.1007/s00299-010-0863-3roots(Guillon et al.2006a,b).A.rhizogenes is a common soil organism capable of infecting plants through a wound site and causing a proliferation of secondary roots,referred to as hairy roots.The underlying mechanism of hairy root formation is the transfer of several bacterial genes from Ri (root-inducing)plasmid in the bacterium to the genome of the infected plant cells(Guillon et al.2006a,b).The hairy root system thus offers tremendous potential for delivering transgenes,and evaluating gene functions in plant roots.This study reports the establishment of a system of A.rhizogenes-mediated hairy root transformation in both wild and domesticated varieties of watermelon.Genetic manipulation using this protocol is rapid,highly efficient, and reproducible.Factors influencing the efficiency of genetic transformation,such as strains of A.rhizogenes and tissue types of watermelon,are also described.Further-more,we examined effect of osmotic stress on the growth of hairy roots derived from these watermelon varieties. Materials and methodsPlant materialsA wild variety of watermelon(Citrullus lanatus sp. No.101117-1),which grows naturally in the Kalahari Desert,has been described previously(Kawasaki et al. 2000).As a reference,we also used a domesticated watermelon cultivar(Citrullus lanatus cv.Sanki)(Maru-tane Breeding,Kyoto,Japan)for hairy root induction.After removing the outer seed coat,these seeds were surface-sterilized for10min with5%(v/v)sodium hypochlorite and0.05%(v/v)Tween-20,and rinsedfive times with sterile water.The sterilized seeds were germinated in vitro and grown for4days on hormone-free Murashige and Skoog(MS)medium(Murashige and Skoog1962)con-taining0.3%gellan gum,at28°C in the dark. Agrobacterium strains and binary vectorTwo A.rhizogenes strains,ATCC43056(R1000)and ATCC15834,harboring a binary vector pIG121-Hm (Akama et al.1992)were used for co-cultivation experi-ments.The T-DNA of the binary vector contains a neomycin phosphotransferase II(NPTII)cassette for kanamycin resistance and a hygromycin phosphotransfer-ase(hpt)gene cassette for hygromycin resistance,which are located adjacent to the left and right borders,respec-tively.The vector also contains an intron-GUS gene (encoding b-glucuronidase)expression cassette(Ohta et al. 1990)between the NPTII and hpt gene cassettes.The binary vector was introduced into each A.rhizogenes strain via electroporation.A single colony of the transformed bacteria was used to inoculate liquid YEB medium supplemented with kana-mycin(50mg/l)and hygromycin(50mg/l).Bacterial cultures were grown overnight at28°C.Agrobacterium cells were collected by centrifugation at10,0009g for 1min at room temperature.The cells was resuspended in liquid MS medium and adjusted to an OD600of0.4–0.5, prior to transformation.Co-cultivationThe4-day-old cotyledon,hypocotyl,and root tissues were cut into pieces of595mm(cotyledons)or5mm long (hypocotyls and roots),and incubated with the Agrobac-terium cells harboring pIG121-Hm in liquid MS medium for10min at28°C.The explants were transferred to MS medium,and co-cultured for2days at28°C in the dark. Following co-cultivation,the explants were washedfive times with sterile water containing500mg/l cefotaxime to remove the Agrobacteria.They were transferred to selec-tive root induction medium(MS medium supplemented with50mg/l kanamycin or5mg/l hygromycin)for trans-genic hairy root induction.The medium also contained 250mg/l cefotaxime for the elimination of Agrobacteria. The selection and induction steps were conducted at26°C under continuous light from whitefluorescent lamps(50–100l mol photons m-2s-1).Genomic DNA extraction and polymerase chain reaction(PCR)amplificationTotal genomic DNA was extracted from the hairy roots by a Plant DNeasy mini kit(Qiagen,Hilden,Germany)and subjected to genomic PCR analysis.Approximately50ng of genomic DNA was used as a template.The primer pair used for the detection of the intron-GUS gene expression cassette was:35S-F,50-ACCCTTCCTCTATATAAG GAAG-30,and GUS-01R,50-CGTGACATCGGCTTCA AATGG-30.The amplification conditions were:a2-min melting step at94°C followed by30cycles of a30-s melting step at94°C,a30-s annealing step at55°C,and a 30-s elongation step at72°C using1unit of Ex Taq DNA polymerase(Takara Bio,Tokyo,Japan).PCR products were analyzed by electrophoretic separation on1%agarose gels and stained with ethidium bromide.Total RNA extraction and reverse transcription(RT)-PCR amplificationRT-PCR was performed as described previously(Kajikawa et al.2009)with some modifications.Total RNA was extracted from the hairy root by a Plant RNA Isolation Mini kit(Agilent,Wilmington,DE)and subjected to123DNase(Qiagen)treatment for15min at25°C.Subse-quently,the RNA was purified by an RNeasy spin column (Qiagen).One microgram of total RNA was used for reverse transcription using ReverTra Ace-a-(Toyobo, Osaka,Japan).One microliter of the resulting cDNA was added to20l l PCR reactions containing1unit of Ex Taq DNA polymerase.The primers for the detection of the intron-spliced GUS gene transcript were:GUS-exon-F, 50-TCTAGAACATGGATCCCTACAGG-30,and GUS-01R as described above.The sequences for GUS-exon-F and GUS-01R primers correspond to the upstream and downstream regions,respectively,of the intron in the GUS coding region.The primers for the detection of the endogenous actin gene transcript that was used as a RT-PCR control were:CLactin2/7-RT-F,50-CATTCTCC GTTTGGACCTTGCT-30,and CLactin2/7-RT-R,50-TCG TAGTTTTCTCAATGGAGGAACTG-30.The amplifica-tion conditions for the GUS and actin fragments were the same as those for the genomic PCR described above.PCR products were analyzed by electrophoretic separation on 1%agarose gels and stained with ethidium bromide. Histochemical analysisTransient histochemical GUS assays were conducted on tissue explants10days after co-cultivation with A.rhizo-genes harboring pIG121-Hm.Tissues were incubated overnight at37°C in a GUS-staining solution containing 50mM potassium phosphate buffer,pH7.0,5mM DTT, 0.1%Tween-20,0.5mM potassium ferrocyanide,0.5mM potassium ferricyanide,20%methanol,and1mM 5-bromo-4-chloro-3-indoyl-b-D-glucuronide(X-gluc).The tissues were then soaked in70%ethanol for several hours to remove chlorophyll.Quantification of GUS-expressing units was performed by counting the number of blue spots on the tissues.For the induced hairy root lines,the histo-chemical GUS assay was performed3weeks after infec-tion,using the GUS-staining solution as described above. Liquid culture of watermelon hairy rootsTissues of the watermelon hairy roots(approximately0.2–0.5g)were inoculated into100ml of B5liquid medium (Gamborg et al.1968)containing30g/l sucrose,and incubated at26°C in the dark on an orbital shaker at 80rpm.Root growth was assayed essentially as described by Dunlop and Curtis(1991).Briefly,the root tissues were aseptically removed from the liquid culture,and liquid medium on their surface was blotted thoroughly by steril-ized absorbent paper towels.The blotted tissues were weighed in a sterile petri dish aseptically,and then trans-ferred back into the liquid medium.Growth analysis of watermelon hairy roots under high osmotic stress conditionTo analyze the hairy root growth under high osmotic stress, approximately5cm of the watermelon hairy roots were placed on a solid half-strength MS medium supplemented with250mg/l cefotaxime and10%polyethylene glycol (PEG)6,000(a reagent grade for molecular biology; Nakalai tesque,Kyoto,Japan),and cultured for4days in the dark.The rate of hairy root elongation was measured every24h.As a control,the same transgenic lines were placed on a solid half-strength MS medium supplemented with250mg/l cefotaxime without PEG6,000,and the elongation of the hairy toots was monitored.Results and discussionOptimization of the transformation efficiencyby transient GUS spot assayTo determine the optimal condition for transgene expres-sion,transient GUS spot assays were carried out using different tissues of wild watermelon and two A.rhizogenes strains.A GUS gene expression vector pIG121-Hm was used for transformation.This vector has a GUS gene containing a modified intron from the castor bean catalase gene within the coding sequence;therefore,this intron-GUS reporter gene does not give rise to detectable GUS activity in Agrobacterium cells,but produces the functional protein after gene transfer into plant cells(Ohta et al. 1990).Four-day-old cotyledons,hypocotyls,and the basal and apical parts of the roots from wild watermelon were used as target tissues for transformation.Two A.rhizogenes strains,ATCC43056and ATCC15834,harboring the pIG121-Hm vector,were used for transformation (Table1).Ten days after co-culture of each tissue with the A.rhizogenes strain,the tissue segments were subjected to the GUS spot assay.Consequently,the tissues infected with ATCC15834showed more GUS spots than those infected with ATCC43056(Table1).Among tissues infected with ATCC15834,hypocotyls gave rise to the highest number of GUS spots;GUS spots were observed in19out of20 explants(95%)tested in this assay,and the average number of GUS spots was5.9per GUS-positive explant,which was markedly higher than the other tissues examined in this assay.This result suggests that hypocotyl fragments and the ATCC15834strain were the optimal combination of target tissue and A.rhizogenes strain for watermelon transformation.Therefore,this combination was used for the induction of the transgenic hairy roots in the following analyses.123Isolation and characterization of transgenic hairy roots To isolate transgenic hairy roots carrying the intron-GUS reporter gene,co-cultured and washed hypocotyl fragments were incubated on selective root induction medium.Fig-ure 1shows representative images for the kanamycin selection of hairy roots derived from wild and domestic varieties of watermelon.After 2weeks,many roots were induced from the hypocotyl fragments (Fig.1a–d).Thewell-elongated lines ([3cm)on the selective medium (50mg/L kanamycin or 5mg/L hygromycin)were trans-ferred onto fresh selective medium.After 1week,more than 10hairy root lines that showed further elongation (Fig.1e,f)were generated from both varieties of water-melon.Hereafter,hairy root lines induced from the wild variety of watermelon were referred to as ‘‘wk’’(kana-mycin-resistant)and ‘‘wh’’(hygromycin-resistant)lines.Similarly,the kanamycin-and hygromycin-resistant linesTable 1Effect of Agrobacterium strains and tissue types on transient GUS expression in wild watermelon variety Agrobacterium strain Tissue Number of GUS-positive explants a Average number of blue spots in GUS-positive explant ATCC43056Cotyledon 00Hypocotyl 8 1.0Basal root b 00Apical rootc00ATCC15834Cotyledon 00Hypocotyl 19 5.9Basal root b 8 2.6Apical root c122.4a Number of explants in which GUS expression was observed 10days after co-cultivation.For each experiment,20explants were testedb The root region (5mm in length)containing the root tip was used as the apical root cThe root region (5mm in length)proximal to the hypocotyl was use d as the basalrootFig.1a Rhizogenes-mediated transformation of watermelon carrying the intron-GUS reporter gene.After co-incubation with A.rhizogenes,hypocotyl fragments from wild (a )and domesticated (b )varieties of watermelon were placed on MS medium containing kanamycin.Formation of kanamycin-resistant hairy roots from the wild (c )and domesticated (d )varieties was observed 2weeks after co-incubation.Well-elongated lines were transferred onto the fresh selective medium.After 1week,hairy roots lines that showed further elongation (indicated by arrows in e and f for wild and domesticated varieties,respectively)were harvested for analyses by genomic PCR,RT-PCR,and histochemical GUS staining.Scale bar 1cm123induced from the domesticated variety of watermelon were referred to as ‘‘dk’’and ‘‘dh’’lines.The wk and dk lines were subjected to genomic PCR analysis to detect the fragment of the GUS gene expression cassette.Among ten wk lines examined,six showed an amplified DNA band with the expected size for the trans-gene (Table 2).In the case of dk lines,nine out of ten lines showed the expected signal (Table 2).These observations suggested that the transgene was transmitted into the hairy root lines with high frequency.Subsequently,the expression of the GUS gene in the genomic PCR-positive lines was detected by RT-PCR.A band corresponding to the intron-spliced GUS transcript(389bp)was detected from all the 8wk lines,and 8out of the 11dk lines (Fig.2a,c).A band corresponding to the intron-unspliced GUS transcript (579bp)was also clearly detected in most of the lines (Fig.2a,b),suggesting that the castor bean intron was not efficiently spliced from the GUS gene transcript in the watermelon cells.The histo-chemical GUS assays showed that all the RT-PCR-positive lines in wk lines showed GUS staining (Fig.2a).The intensity of the GUS staining was relatively weak in the four of the lines (lines wk-4,wk-5,wk-11and wk-12),in which the intron-unspliced GUS transcript was detected.Among the eight RT-PCR-positive lines in domesticated watermelon,four lines showed strong GUS staining (dk-1,dk-3,dk-7and dk-10),one line showed partial GUS staining (dk-6),two lines showed weak GUS signal (dk-11and dk-12)and the other three lines did not show any detectable GUS staining (dk-4,dk-8and dk-9)(Fig.2b).The dk-2line,which had no detectable RT-PCR product,also did not show any GUS staining.In the genomic PCR-negative wk and dk lines,neither RT-PCR nor GUS staining was detected (data not shown).These results demonstrated that the transgenic hairy roots from both wild and domesticated watermelon plants with high expression of the GUS transgene were isolated efficiently in this screening.In the hygromycin selection,nine and eight of the 10wh and dh lines,respectively,were genomic PCR-positive (Table 2).RT-PCR experiments showed that the intron-spliced GUS transcript was detected in all the genomic PCR-positive wh and dh lines (Fig.2b,d).Similar to the kanamycin selection,the intron-remaining GUS transcriptTable 2Screening of the transgenic hairy roots Antibiotics Variety Genomic PCR a RT-PCR b GUS staining c Kanamycin Wild 6/106/66/6Domesticated 9/108/95/9HygromycinWild 9/109/97/9Domesticated8/108/85/8aThe denominator and numerator in each fraction designate the number of hairy root lines assayed and detected,respectively,for the presence of intron-GUS transgene by genomic PCRbThe denominator and numerator in each fraction designate the number of hairy root lines assayed and detected,respectively,for the presence of spliced GUS transcripts by RT-PCRcThe denominator and numerator in each fraction designate the number of hairy root lines assayed and detected,respectively,for the presence of functional GUS gene product by histochemical GUS stainingunspliced splicedunspliced GUS GUS stainingstaining6 1 2 3 567 89 10(vc)123478910111212 3 457810(vc)(vc)unspliced splicedunspliced lines lines dk dh1458 9101112(vc)GUS staininglines histochemical analyses of GUS gene expression roots.The expression of GUS reporter genes and -unspliced (579bp)GUS the right side of each gel image.123was detected in five out of nine wh RT-PCR positive-lines and all the dh RT-PCR-positive lines (Fig.2b,d).In the histochemical GUS assays,strong GUS staining was detected in four wh lines (wh-1,wh-3,wh-7and wh-10),in which only the intron-spliced GUS transcript was detected.The remaining five wh lines that accumulated the intron-unspliced GUS transcript showed either weak (wh-2,wh-5and wh-9),or no GUS staining (wh-6and wh-8).In the case of dh lines,two (dh-3and dh-7),three (dh-2,dh-4and dh-10),and two lines (dh-5and dh-8)showed strong,weak and no GUS staining,respectively,among the eight RT-PCR-positive lines.These results demonstrated that hygromycin selection of the transgenic hairy roots is practicable,in contrast to a previous report that transgenic plants were not obtained by hygromycin selection in A.tumefaciens -mediated trans-formation of wild variety of watermelon (Akashi et al.2005).In the present study,a positive correlation was observed between the efficiency of splicing of the intron in the GUS transgene,and the strength of the GUS signals (Fig.2).Interestingly,several transgenic lines with detectable intron-spliced GUS transcript showed no GUS staining (Fig.2),suggesting that the GUS expression was suppressed post-transcriptionally in these hairy root lines.Although further investigations are necessary to reveal the mechanism of post-transcriptional silencing of theheterologous gene expression in these transgenic water-melon plants,the present study highlights the importance of examining protein expression and/or protein activity to establish the transgenic hairy root lines.Proliferation of the transgenic hairy roots in the liquid cultureA liquid culture system provides rapid and effective pro-duction of the transgenic hairy roots (Giri and Narasu 2000;Triplett et al.2008);therefore,it might offer a powerful tool for the physiological study of watermelon root tissues.To establish liquid cultures of the transgenic watermelon hairy roots,several lines with relatively rapid growth rates in the kanamycin-resistant lines (wk-11and wk-12lines from the wild variety,and dk-11and dk-12lines from the domesticated variety)were transferred into 100ml of B5liquid medium without the antibiotic (Fig.3).The hairy root lines grew rapidly in the liquid medium.After 14days,the fresh weight of the hairy roots in each culture increased by 8-to 18-fold in comparison to their starting weight (Fig.3).The average growth rates (g/day)were 0.81±0.36and 1.25±0.33for wk-11and wk-12,respectively,and 0.46±0.18and 0.52±0.08for dk-11and dk-12,respectively.The average growth rate of the wk-12line was different significantly from those of the(a)F r e s h w e i g h t (g /c u l t u r e )Incubation time (days)012wk-11wk-12dk-11dk-12incubation time (days)l i n e s(b)04914012incubation time (days)Fig.3Time course analysis of the hairy root growth in liquid culture.The growth of transformed kanamycin-resistant hairy root cultures of watermelon.In a ,the hairy root cultures at the beginning and 14days after the inoculation are shown for hairy root lineswk-11and wk-12derived from wild variety of watermelon,and hairy root lines dk-11and dk-12from the domesticated variety.At regular intervals,hairy roots were recovered from the medium and weighedaseptically.In the graph of b ,filled circle and diamond designate fresh weight of the hairy roots for wk-11and wk-12lines,respectively,whereas open circle and diamond designate those for dk-11and dk-12lines,respectively.Data indicate the mean values ±SD from three replicates123dk-11(t test,P \0.05)and dk-12lines (t test,P \0.05),whereas the average growth rate of wk-11line was statis-tically insignificant compared to those of the dk lines (t test,P [0.05).The high growth rate of these hairy root lines was maintained for several passages of the liquid culture (data not shown).Growth of the transgenic hairy roots under high osmotic conditionTo examine the effect of environmental stress on the growth of watermelon hairy roots,two hairy root lines derived from wild watermelon (wk-11and wk-12),and two lines from domesticated watermelon (dk-11and dk-12)were grown under the high osmotic condition.Osmotic treatment was administrated using PEG 6,000at a final concentration of 10%on the solid MS medium for 4days.Differential response was found between the wk and dk lines (Fig.4).In the dk lines derived from domesticated variety of watermelon,rate of root growth elongation strongly dropped from 0.22±0.01and 0.28±0.07cm/day on control medium,to 0.05±0.001(23%of the control medium;t test,P \0.01)and 0.03±0.03cm/day (9%;t test,P \0.01)on 10%PEG medium for dk-11and dk-12lines,respectively.These observations demonstrated very strong inhibition of root growth under high osmotic condition in the dk lines.By contrast,degree of inhibition was markedly small in the wk lines derived from the wild variety of watermelon.Growth rates of wk-11and wk-12lines on 10%PEG medium were 0.25±0.08(51%of the control medium;t test,P \0.05)and 0.32±0.06cm/day (75%of the control;t test,P [0.05),respectively,which were modestly slower or statistically insignificant com-pared to those in the control medium (0.50±0.06and 0.43±0.10cm/day for the wk-11and wk-12lines,respectively).In this study,an efficient protocol for the generation of transgenic hairy roots was established from two different varieties of watermelon plants,the one for drought-resis-tant wild variety,and the other for drought-susceptible domesticated variety.The new protocol presented in this study will be useful in comparative functional analysis of the genes in wild and domesticated watermelon,which show significant differences in root vigor under water deficits.The future applications of this protocol would include loss-of-function experiments of a gene in question by RNAi or antisense techniques,gain-of-function experi-ments of a gene by overexpression,and measurement of gene expression with chimeric promoter–reporter systems.It is anticipated that these approaches would yield invalu-able insights into the molecular mechanisms of root vigor in the desert plant under drought.Acknowledgments The authors gratefully acknowledge Ms.Ma-sayo Inoue for her technical contribution and Dr.Kazuhito Akama (Shimane University)for providing us with the pIG121-Hm vector plasmid.This work was financially supported partly by the Japan Science and Technology Agency (JST),partly by the Nissan Science Foundation,partly by a Grants-in-Aid for Scientific Research,Japan Society for the Promotion of Science,the Ministry of Education,Science,Sports and Culture,and partly by a grant from Foundation for Nara Institute of Science and Technology.ReferencesAkama K,Shiraishi H,Ohta S,Nakamura K,Okada K,Shimura Y(1992)Efficient transformation of Arabidopsis thaliana :com-parison of the efficiencies with various organs,plant ecotypes and Agrobacterium strains.Plant Cell Rep 12:7–11Akashi K,Morikawa K,Yokota A (2005)Agrobacterium -mediatedtransformation system for the drought and excess light stress-tolerant wild watermelon (Citrullus lanatus ).Plant Biotechnol 22:13–18Akashi K,Yoshimura K,Nanasato Y,Takahara K,Munekage Y,Yokota A (2008)Wild plant resources for studying molecularE l o n g a t i o n o f h a i r y r o o t (c m )E l o n g a t i o n o f h a i r y r o o t (c m )culture time (days)01234culture time (days)01234Fig.4Time course analysis of the hairy root growth in high osmotic condition.The elongation of hairy roots after transfer on a half-strength MS medium supplemented polyethylene glycol are shown for hairy roots derived from the wild (a )and domesticated (b )variety watermelon.Closed circles and triangles designate for wk-11and wk-12(a ),and dk-11and dk-12(b ),respectively,on the medium supplemented 10%PEG 6000.Open circles and triangles designate for wk-11and wk-12(a ),and dk-11and dk-12(b ),respectively,on the normal medium as control.Data indicate the mean values ±SD from three replicates123mechanisms of drought/strong light stress tolerance.Plant Biotechnol25:257–263Choi PS,Soh WY,Kim YS,Yoo OJ,Liu JR(1994)Genetic transformation and plant regeneration of watermelon using Agrobacterium tumefaciens.Plant Cell Rep13:344–348 Dunlop SD,Curtis WR(1991)Synergistic response of plant hairy-root cultures to phosphate limitation and fungal elicitation.Biotechnol Prog7:434–438Ellul P,Rios G,Atares A,Roig LA,Serrano R,Moreno V(2003)The expression of the Saccharomyces cerevisiae HAL1gene increases salt tolerance in transgenic watermelon[Citrullus lanatus(Thumb.)Matsun&Nakai.].Theor Appl Genet 107:462–469Gamborg OL,Miller RA,Ojima K(1968)Nutrient requirements of suspension cultures of soybean root cells.Exp Cell Res50:151–158Giri A,Narasu L(2000)Transgenic hairy roots:recent trends and applications.Biotechnol Adv18:1–22Guillon S,Tre´mouillaux-Guiller J,Pati PK,Rideau M,Gantet P (2006a)Harnessing the potential of hairy roots:dawn of a new era.Trends Biotechnol24:403–409Guillon S,Tre´mouillaux-Guiller J,Pati PK,Rideau M,Gantet P (2006b)Hairy root research:recent scenario and exciting prospects.Curr Opin Plant Biol9:341–346Kajikawa M,Hirai H,Hashimoto T(2009)A PIP-family reductase is required for biosynthesis of tobacco alkaloids.Plant Mol Biol 69:287–298Kawasaki S,Miyake C,Kohchi T,Fujii S,Uchida M,Yokota A (2000)Responses of wild watermelon to drought stress:accu-mulation of an ArgE homologue and citrulline in leaves during water deficits.Plant Cell Physiol41:864–873Kohzuma K,Cruz JA,Akashi K,Hoshiyasu S,Munekage YN, Yokota A,Kramer DM(2009)The long-term responses of the photosynthetic proton circuit to drought.Plant Cell Environ 32:209–219Larcher W(1995)Plants under stress.In:Physiological plant ecology.Springer,Berlin,pp321–448Murashige T,Skoog F(1962)A revised medium for rapid growth and bioassays with tobacco tissue cultures.Physiol Plant15:473–497 Ohta S,Mita S,Hattori T,Nakamura K(1990)Construction and expression in tobacco of a b-glucuronidase(GUS)reporter gene containing an intron within the coding sequence.Plant Cell Physiol31:805–813Takahara K,Akashi K,Yokota A(2005)Purification and character-ization of glutamate N-acetyltransferase involved in citrulline accumulation in wild watermelon.FEBS J272:5353–5364 Triplett BA,Moss SC,Bland JM,Dowd MK(2008)Induction of hairy root cultures from Gossypium hirsutum and Gossypium barbadense to produce gossypol and related compounds.In Vitro Cell Dev Biol Plant44:508–517Yoshimura K,Masuda A,Kuwano M,Yokota A,Akashi K(2008) Programmed proteome response for drought avoidance/tolerance in the root of a C3xerophyte(wild watermelon)under water deficits.Plant Cell Physiol49:226–241123。
Multiplicative Controllability of the Semilinear
bilinear system (3.1) and of its nonhomogeneous version.
r1
∈
[0, 1 + 4 ), n
r2
∈
[0,
1
+
n
2 +
2
)
(3.2)
A.Y. Khapalov, Controllability of Partial Differential Equations Governed
33
by Multiplicative Controls, Lecture Notes in Mathematics 1995,
origin.
Our central idea below is to view the evolution of system (3.1) as an interaction of the following three dynamics associated with the respective three terms in the right-hand side of (3.1):
∂z ∂t
=
Δz
+
v(z − θ (x))
−
f (x,t, z, ∇z) in
QT ,
(3.5)
z = 0 in ΣT , z |t=0 = z0 ∈ L2(Ω ),
where f does not necessarily vanish at the origin and θ = 0 is given.
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人类基因组概况ppt课件
2.91Gbp
54% 38% 9% 35% 26588 42% Titin(234) 约300万个 1/12500 bp
最长的染色体 最短的染色体 基因最多的染色体 基因最少的染色体 基因密度最大的染色体 基因密度最小的染色体 重复序列含量最高的染色体
It is essentially immoral not to get it (the human genome sequence) done as fast as possible.
James Watson
人类基因组计划的完成,使得我们今天有可能来探 讨基因组的概,但我们仍然无法来谈论细节。
重复序列含量最低的染色体
编码外显子序列的比例 基因的平均长度
2(240 Mbp) Y(19 Mbp) 1(2453) Y(104) 19(23/Mb) 13,Y(5/Mb) 19(57%)
2,8,10,13,18(36%)
1.1~1.4% 27 Kb
女 平均 男
染色体上距着丝粒越远,重组率越高
4. Francis S. Collins, Eric D. Green, Alan E. Guttmacher, Mark S. Guyer :A Vision for the Future of Genomics Research. A blueprint for the genomic era. Nature Apr 24 2003: 835.
而 Celera 的测序样本来自5个人:分别属于西班牙裔、 亚洲裔、非洲裔、美洲裔和高加索裔(2男3女),是从21个志 愿者样本中挑选的。
土木类SCI期刊
Rank ISSN Abbreviated Journal Title32150718-915X REV CONSTR32790733-9445J STRUCT ENG45331016-8664STRUCT ENG INT61851464-4177STRUCT CONCRETE80941940-1493J BUILD PERFORM SIMU 32760733-9402J ENERG ENG32810733-947X J TRANSP ENG28680350-2465GRADEVINAR32800733-9453J SURV ENG32820733-9488J URBAN PLAN DEV 50751076-0342J INFRASTRUCT SYST 54611226-7988KSCE J CIV ENG59651392-3730J CIV ENG MANAG63931478-4629P I CIVIL ENG-ENG SU 73661735-0522INT J CIV ENG85572228-6160IJST-T CIV ENG2270005-6650BAUINGENIEUR-GERMANY 4570013-8029ENG J AISC12790038-9145STAHLBAU14160049-4488TRANSPORTATION17320141-0296ENG STRUCT17800143-974X J CONSTR STEEL RES 19560167-4730STRUCT SAF19660167-6105J WIND ENG IND AEROD 21340191-2615TRANSPORT RES B-METH 21880197-6729J ADV TRANSPORT23960263-8231THIN WALL STRUCT 27910315-1468CAN J CIVIL ENG30640378-7788ENERG BUILDINGS31660553-6626PERIOD POLYTECH-CIV 35310885-7024CIVIL ENG35490886-7798TUNN UNDERGR SP TECH 35570887-3801J COMPUT CIVIL ENG 35590887-3828J PERFORM CONSTR FAC 36550893-1321J AEROSPACE ENG38980926-5805AUTOMAT CONSTR39730932-8351BAUTECHNIK43280965-089X P I CIVIL ENG-CIV EN 43290965-0903P I CIVIL ENG-MUNIC 43300965-0911P I CIVIL ENG-STR B 43310965-092X P I CIVIL ENG-TRANSP 45571021-2019J S AFR INST CIV ENG 46231028-6608CIV ENG ENVIRON SYST 51571084-0702J BRIDGE ENG52461093-9687COMPUT-AIDED CIV INF 54591226-6116WIND STRUCT55411300-3453TEK DERGI58091366-5545TRANSPORT RES E-LOG 58351369-4332ADV STRUCT ENG 66921541-7794STRUCT DES TALL SPEC 68741558-3058INT J ARCHIT HERIT 70521598-2351INT J STEEL STRUCT 77671822-427X BALT J ROAD BRIDGE E 2390006-3207BIOL CONSERV 4620013-9157ENVIRONMENT 11300032-2474POLAR REC13850045-6535CHEMOSPHERE 14010047-2425J ENVIRON QUAL 14120048-9697SCI TOTAL ENVIRON 16270098-8472ENVIRON EXP BOT 17230140-1963J ARID ENVIRON 18600160-4120ENVIRON INT 19090165-0009CLIMATIC CHANGE 19110165-0203NAT RESOUR FORUM 19680167-6369ENVIRON MONIT ASSESS 24160265-931X J ENVIRON RADIOACTIV 24710269-7491ENVIRON POLLUT 25330275-7540CHEM ECOL25480277-5212WETLANDS26550301-4797J ENVIRON MANAGE 29430364-152X ENVIRON MANAGE 30210376-8929ENVIRON CONSERV 31210393-5965AEROBIOLOGIA 34250791-7945BIOL ENVIRON 35850888-8892CONSERV BIOL 36220890-8575NAT RESOUR MODEL 36410892-0753COAST MANAGE 38340921-8009ECOL ECON40500944-1344ENVIRON SCI POLLUT R 42080957-4352INT J ENVIRON POLLUT 42320959-3330ENVIRON TECHNOL 42330959-3780GLOBAL ENVIRON CHANG 42630960-3115BIODIVERS CONSERV 44561001-0742J ENVIRON SCI-CHINA 44651002-0063CHINESE GEOGR SCI 45431018-4619FRESEN ENVIRON BULL 47731051-0761ECOL APPL49411064-3389CRIT REV ENV SCI TEC 51231080-7039HUM ECOL RISK ASSESS 51861088-1980J IND ECOL54761230-1485POL J ENVIRON STUD 55761311-5065J ENVIRON PROT ECOL 57091352-2310ATMOS ENVIRON 57201354-1013GLOBAL CHANGE BIOL 60811436-3798REG ENVIRON CHANGE 60941438-4957J MATER CYCLES WASTE 62351470-160X ECOL INDIC64731522-6514INT J PHYTOREMEDIAT65331527-5922ENVIRON FORENSICS 66791540-9295FRONT ECOL ENVIRON 67121543-5938ANNU REV ENV RESOUR 70271582-9596ENVIRON ENG MANAG J 70981612-9202ECOHEALTH71631648-6897J ENVIRON ENG LANDSC 72261672-6316J MT SCI-ENGL 73721735-1472INT J ENVIRON SCI TE 75291748-9326ENVIRON RES LETT 77191790-7632GLOBAL NEST J 77901842-4090CARPATH J EARTH ENV 78211862-4065SUSTAIN SCI 79731898-6196ECOL CHEM ENG SFull TitleRevista de la ConstrucciónJOURNAL OF STRUCTURAL ENGINEERINGStructural Engineering InternationalStructural ConcreteJournal of Building Performance SimulationJOURNAL OF ENERGY ENGINEERINGJOURNAL OF TRANSPORTATION ENGINEERINGGRADEVINARJOURNAL OF SURVEYING ENGINEERINGJOURNAL OF URBAN PLANNING AND DEVELOPMENTJOURNAL OF INFRASTRUCTURE SYSTEMSKSCE JOURNAL OF CIVIL ENGINEERINGJOURNAL OF CIVIL ENGINEERING AND MANAGEMENTPROCEEDINGS OF THE INSTITUTION OF CIVIL ENGINEERS-ENGINEERING SUSTAINA INTERNATIONAL JOURNAL OF CIVIL ENGINEERINGIranian Journal of Science and Technology-Transactions of Civil Engine BauingenieurENGINEERING JOURNAL-AMERICAN INSTITUTE OF STEEL CONSTRUCTION INC StahlbauTRANSPORTATIONENGINEERING STRUCTURESJOURNAL OF CONSTRUCTIONAL STEEL RESEARCHSTRUCTURAL SAFETYJOURNAL OF WIND ENGINEERING AND INDUSTRIAL AERODYNAMICS TRANSPORTATION RESEARCH PART B-METHODOLOGICALJOURNAL OF ADVANCED TRANSPORTATIONTHIN-WALLED STRUCTURESCANADIAN JOURNAL OF CIVIL ENGINEERINGENERGY AND BUILDINGSPeriodica Polytechnica-Civil EngineeringCIVIL ENGINEERINGTUNNELLING AND UNDERGROUND SPACE TECHNOLOGYJOURNAL OF COMPUTING IN CIVIL ENGINEERINGJOURNAL OF PERFORMANCE OF CONSTRUCTED FACILITIESJOURNAL OF AEROSPACE ENGINEERINGAUTOMATION IN CONSTRUCTIONBautechnikPROCEEDINGS OF THE INSTITUTION OF CIVIL ENGINEERS-CIVIL ENGINEERING PROCEEDINGS OF THE INSTITUTION OF CIVIL ENGINEERS-MUNICIPAL ENGINEER PROCEEDINGS OF THE INSTITUTION OF CIVIL ENGINEERS-STRUCTURES AND BUILD PROCEEDINGS OF THE INSTITUTION OF CIVIL ENGINEERS-TRANSPORTJournal of the South African Institution of Civil EngineeringCIVIL ENGINEERING AND ENVIRONMENTAL SYSTEMSJournal of Bridge EngineeringCOMPUTER-AIDED CIVIL AND INFRASTRUCTURE ENGINEERINGWIND AND STRUCTURESTeknik DergiTRANSPORTATION RESEARCH PART E-LOGISTICS AND TRANSPORTATION REVIEW ADVANCES IN STRUCTURAL ENGINEERINGSTRUCTURAL DESIGN OF TALL AND SPECIAL BUILDINGSInternational Journal of Architectural HeritageInternational Journal of Steel StructuresBaltic Journal of Road and Bridge EngineeringBIOLOGICAL CONSERVATIONENVIRONMENTPOLAR RECORDCHEMOSPHEREJOURNAL OF ENVIRONMENTAL QUALITYSCIENCE OF THE TOTAL ENVIRONMENTENVIRONMENTAL AND EXPERIMENTAL BOTANYJOURNAL OF ARID ENVIRONMENTSENVIRONMENT INTERNATIONALCLIMATIC CHANGENATURAL RESOURCES FORUMENVIRONMENTAL MONITORING AND ASSESSMENTJOURNAL OF ENVIRONMENTAL RADIOACTIVITYENVIRONMENTAL POLLUTIONCHEMISTRY AND ECOLOGYWETLANDSJOURNAL OF ENVIRONMENTAL MANAGEMENTENVIRONMENTAL MANAGEMENTENVIRONMENTAL CONSERVATIONAEROBIOLOGIABIOLOGY AND ENVIRONMENT-PROCEEDINGS OF THE ROYAL IRISH ACADEMY CONSERVATION BIOLOGYNATURAL RESOURCE MODELINGCOASTAL MANAGEMENTECOLOGICAL ECONOMICSENVIRONMENTAL SCIENCE AND POLLUTION RESEARCHINTERNATIONAL JOURNAL OF ENVIRONMENT AND POLLUTION ENVIRONMENTAL TECHNOLOGYGLOBAL ENVIRONMENTAL CHANGE-HUMAN AND POLICY DIMENSIONS BIODIVERSITY AND CONSERVATIONJOURNAL OF ENVIRONMENTAL SCIENCES-CHINAChinese Geographical ScienceFRESENIUS ENVIRONMENTAL BULLETINECOLOGICAL APPLICATIONSCRITICAL REVIEWS IN ENVIRONMENTAL SCIENCE AND TECHNOLOGYHUMAN AND ECOLOGICAL RISK ASSESSMENTJOURNAL OF INDUSTRIAL ECOLOGYPOLISH JOURNAL OF ENVIRONMENTAL STUDIESJournal of Environmental Protection and EcologyATMOSPHERIC ENVIRONMENTGLOBAL CHANGE BIOLOGYRegional Environmental ChangeJournal of Material Cycles and Waste ManagementECOLOGICAL INDICATORSINTERNATIONAL JOURNAL OF PHYTOREMEDIATIONENVIRONMENTAL FORENSICSFRONTIERS IN ECOLOGY AND THE ENVIRONMENTANNUAL REVIEW OF ENVIRONMENT AND RESOURCESEnvironmental Engineering and Management JournalECOHEALTHJournal of Environmental Engineering and Landscape ManagementJournal of Mountain ScienceInternational Journal of Environmental Science and Technology Environmental Research LettersGlobal NEST JournalCarpathian Journal of Earth and Environmental SciencesSustainability ScienceEcological Chemistry and Engineering S-Chemia I Inzynieria EkologicznaCategory Subcategory CountryCONSTRUCTION & BUILDING TECHNOLOGY ENGINEERING, CIVIL CHILE CONSTRUCTION & BUILDING TECHNOLOGY ENGINEERING, CIVIL UNITED STATES CONSTRUCTION & BUILDING TECHNOLOGY ENGINEERING, CIVIL SWITZERLAND CONSTRUCTION & BUILDING TECHNOLOGY ENGINEERING, CIVIL GERMANY CONSTRUCTION & BUILDING TECHNOLOGY ENGLAND ENERGY & FUELS ENGINEERING, CIVIL UNITED STATES ENGINEERING, CIVIL TRANSPORTATION SCIENCE & TECHNOLOGY UNITED STATES ENGINEERING, CIVIL CROATIA ENGINEERING, CIVIL UNITED STATES ENGINEERING, CIVIL UNITED STATES ENGINEERING, CIVIL UNITED STATES ENGINEERING, CIVIL SOUTH KOREA ENGINEERING, CIVIL LITHUANIA ENGINEERING, CIVIL ENGLAND ENGINEERING, CIVIL IRAN ENGINEERING, CIVIL IRAN工程技术工程:土木GERMANY工程技术工程:土木UNITED STATES 工程技术工程:土木GERMANY工程技术工程:土木NETHERLANDS 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1.5240.7180.0000.0000.000 318 1.343 1.1040.0000.0000.0000.0000.000 23350.7970.8770.0000.0000.0000.0000.000 720.2020.2160.1050.0820.0470.0000.000 320 1.000 1.0000.0000.0000.0000.0000.000 5140.8090.9310.0000.0000.0000.0000.000 832 1.0490.6480.9830.6540.7400.0000.000 5580.4840.5110.3830.3770.4500.0000.000 535 1.070 1.372 2.016 2.171 3.7110.0000.000 1790.7680.6040.5200.5870.5220.0000.000 1510.4680.3970.3790.6950.5470.0000.000 210.3330.1820.0000.0000.0000.0000.000 2410.5110.2070.1960.1500.1340.1940.000 2140.143 1.3710.2270.3490.2380.1840.412 3400.2190.2250.2560.2540.2340.3000.000 1945 2.358 1.617 1.657 1.023 1.875 1.512 1.767 9954 1.838 1.767 1.713 1.351 1.363 1.256 1.102 3910 1.321 1.370 1.327 1.251 1.003 1.0180.841 1716 1.675 2.391 1.840 1.867 1.770 2.276 1.467 4926 1.414 1.698 1.342 1.119 1.2130.8310.752 6014 2.952 3.894 2.944 2.856 2.091 2.268 1.874 554 1.606 1.8780.7330.6430.6500.727 1.207 3392 1.749 1.432 1.231 1.252 1.103 1.0540.788 18800.5560.4070.4720.3340.4010.4020.291 13417 2.884 2.465 2.679 2.386 2.041 1.593 1.590 570.2610.2500.2330.4550.0770.2220.000 2470.2690.1670.0790.1760.1620.0800.151 2204 1.490 1.589 1.1060.7270.9170.8600.710 1277 1.268 1.385 1.268 1.3370.900 1.012 1.114 6450.6310.5920.5760.4500.2930.3200.500 6600.8390.9260.7780.6970.4200.7140.907 2637 1.812 1.822 1.820 1.500 1.311 1.372 1.664 2340.1740.3170.2930.1760.1410.2100.000 2150.7140.4430.2940.1250.0850.0870.131 1250.1480.2410.2540.2090.4620.2240.138 4110.4940.2820.6090.5730.3730.4240.328 1680.2620.3210.2950.3260.1910.1740.136 720.3150.2220.2960.1820.3570.1250.000 2300.5120.7250.4770.5620.5950.5000.425 1330 1.0650.9270.7930.623 1.0090.4600.438 1834 4.925 5.625 4.460 3.382 3.170 1.9890.747 4030.5840.905 1.2540.5080.6350.5410.548 240.0230.1180.1500.1180.0300.0320.0002947 2.676 2.193 2.272 1.648 1.954 1.958 1.270 5740.5820.6030.4890.3240.4630.4650.513 6470.9440.9570.829 1.0410.5050.2690.388 1770.5610.7140.3700.2350.5000.5620.000 2220.5050.4710.2970.4260.3620.2710.000 1460.766 1.053 1.478 1.610 2.436 2.056 1.600 21429 3.762 4.036 3.794 4.115 3.498 3.167 3.566 711 1.545 1.353 1.375 1.738 1.864 1.918 1.444 3780.6670.6210.9810.9620.8890.5820.583 47655 3.340 3.499 3.137 3.206 3.155 3.253 3.054 13085 2.652 2.345 2.353 2.324 2.236 2.291 2.098 39706 4.099 3.163 3.258 3.286 3.190 2.905 2.579 6524 3.359 3.003 2.578 2.985 2.699 3.164 2.301 6660 1.641 1.822 1.772 1.723 1.535 1.426 1.589 12067 5.559 5.664 6.248 5.297 4.691 4.786 3.516 13208 3.430 4.622 3.634 3.385 3.016 3.635 3.202 618 1.146 1.0000.9800.667 1.0640.6880.792 10736 1.679 1.679 1.592 1.400 1.436 1.356 1.035 4861 2.483 3.571 2.119 1.339 1.466 1.268 1.114 26921 4.143 3.902 3.730 3.746 3.395 3.426 3.135 801 1.047 1.180 1.0690.6150.7760.6340.838 3439 1.572 1.444 1.283 1.338 1.238 1.328 1.117 14363 2.723 3.188 3.057 3.245 2.596 2.367 1.794 6909 1.724 1.648 1.647 1.744 1.503 1.408 1.109 2654 2.368 2.320 2.341 1.927 2.000 1.541 1.875 926 1.375 1.202 1.333 1.515 1.052 1.173 1.086 2350.6740.6190.6560.4150.4250.0000.041 17614 4.165 4.320 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1.984 1137 1.739 1.466 1.179 1.298 1.936 1.321 1.2174080.5620.7320.6520.7680.6810.529 1.077 62057.4418.4127.6159.1138.820 6.922 5.065 2122 5.892 5.056 4.968 6.419 3.737 3.657 4.667 1268 1.065 1.258 1.117 1.004 1.4350.8850.000 1164 2.451 2.267 2.196 1.702 1.640 2.089 2.315 2090.6230.732 1.041 1.958 1.333 1.5080.000 6110.9630.7630.664 1.0000.6320.4000.000 1680 2.190 1.794 1.844 3.051 3.157 1.4170.000 4998 3.906 4.090 3.582 3.631 3.049 3.342 1.719 4790.4680.6600.6980.5360.4500.5650.000 2810.6300.727 1.495 1.450 1.5790.6060.286 568 3.119 3.372 2.1890.886 1.222 1.6510.000 2190.5530.5580.3820.4230.2940.6150.000IF 2007-20085-YearImpactFactorImmediacyIndexArticlesCitedHalf-LifeEigenfactor ScoreArticleInfluenceScoreJCR-2014收录中科院JCR大类学科0.0000.1870.000150.000090.040收录工程:土木0.000 1.9110.316225>10.00.010860.842收录工程:土木0.0000.0000.018577.70.000940.000收录工程:土木0.000 1.1230.15751 3.90.001040.537收录工程:土木0.000 2.1750.65529 3.50.001100.608收录结构与建筑技术0.000 1.2880.15645 6.00.000430.227收录工程:土木0.000 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5.00.020150.642收录工程:土木0.0000.4550.026390.000200.122收录工程:土木0.2210.2080.03951>10.00.000350.091收录工程:土木0.408 1.8330.247158 6.90.003880.501收录工程:土木0.707 1.6160.362698.70.002670.590收录工程:土木0.2440.8730.109101 6.50.001700.315收录工程:土木0.5850.9860.202109 6.70.001580.348收录工程:土木0.609 2.4140.281171 4.90.006700.633收录工程:土木0.0000.2070.287877.70.000880.151收录工程:土木0.1080.535 1.12532 3.90.000460.170收录工程:土木0.1030.2370.79224 6.80.000190.078收录工程:土木0.2710.553 1.098617.60.000840.277收录工程:土木0.1090.511 1.05735 4.90.000340.165收录工程:土木0.0000.3640.097310.000170.120收录工程:土木0.2220.8550.000247.40.000440.252收录工程:土木0.000 1.2310.1381307.00.002860.404收录工程:土木0.861 4.0210.48050 5.40.003590.943收录工程:土木0.5410.8220.02773 6.90.000780.248收录工程:土木0.0000.1020.000160.000050.027收录工程:土木1.000 3.5130.404141 5.60.00787 1.136收录工程:土木0.2670.6850.089124 4.40.002080.242收录工程:土木0.159 1.2190.20578 4.80.001570.326收录工程:土木0.0000.7790.25547 4.10.000590.273收录工程:土木0.0000.6590.01376 4.80.000750.219收录工程:土木0.8000.7030.10329 3.40.000370.135收录工程:土木3.296 4.6970.6723057.90.04415 1.686收录环境科学1.2932.2610.312168.50.001440.985收录环境科学0.4150.7350.05040>10.00.000600.233收录环境科学2.7393.8540.5719237.40.067030.934收录环境科学0.000 2.9720.703212>10.00.015000.881收录环境科学2.1824.4140.9211751 6.30.07020 1.126收录环境科学1.810 3.746 1.068148 6.60.010680.901收录环境科学1.3492.0560.3281318.70.009520.627收录环境科学2.797 6.657 1.047254 6.50.02300 1.784收录环境科学2.890 4.6100.6702737.00.03837 1.916收录环境科学0.709 1.4830.042249.30.001000.525收录环境科学0.885 1.9180.229695 5.20.022160.441收录环境科学0.963 2.3220.309259 6.90.007560.560收录环境科学3.1354.7550.9914417.00.04408 1.213收录环境科学0.475 1.1690.13865 6.80.001240.258收录环境科学0.973 1.9190.3071278.70.005000.581收录环境科学1.446 3.8950.470396 5.30.03805 1.059收录环境科学1.2402.3400.3791959.30.011310.737收录环境科学1.1433.0840.14734>10.00.00342 1.125收录环境科学0.944 1.5560.237389.00.001200.383收录环境科学0.0200.7310.000258.70.000280.169收录环境科学3.934 5.1990.935168>10.00.02382 2.065收录环境科学0.3830.9910.000258.50.000810.438收录环境科学0.911 1.213 1.345297.80.000940.339收录环境科学1.549 3.9290.3392397.10.02571 1.292收录环境科学3.8942.9200.3671292 2.50.017180.652收录环境科学0.4350.4490.020498.30.000920.117收录环境科学0.735 1.5910.362354 6.20.005910.316收录环境科学3.9157.7840.969161 5.70.02222 2.849收录环境科学1.421 2.6760.5372017.60.015610.899收录环境科学0.480 2.5330.195293 5.20.013240.590收录环境科学0.0000.9060.373674.70.001060.209收录环境科学0.4290.4190.0314545.40.002240.062收录环境科学3.571 5.5080.7111599.50.03025 2.052收录环境科学4.615 4.6510.623618.00.00470 1.290收录环境科学0.912 1.2550.415948.40.002150.359收录环境科学1.962 3.695 1.12572 5.80.00656 1.164收录环境科学0.6270.8880.0922827.50.002560.168收录环境科学0.0000.6110.038211 3.00.000380.022收录环境科学2.5493.7800.6038187.90.06290 1.041收录环境科学4.7868.708 1.575308 6.10.07007 3.160收录环境科学0.000 2.9460.571163 3.30.004640.870收录环境科学0.0000.9760.26284 5.60.000910.229收录环境科学1.576 3.4940.698444 3.00.016860.888收录环境科学1.489 1.8750.34188 4.70.002350.419收录环境科学1.4120.7740.000308.80.000510.182收录环境科学4.2699.878 1.64562 6.20.01792 3.924收录环境科学4.0369.3720.348237.50.00529 3.864收录环境科学0.0000.8060.206325 2.90.001420.069收录环境科学1.4922.6180.72236 5.60.003070.828收录环境科学0.0000.7850.03132 4.80.000400.144收录环境科学0.000 1.1110.083132 4.30.001370.238收录环境科学0.000 2.4910.300227 4.30.003460.480收录环境科学1.200 4.4190.563327 3.10.02683 1.825收录环境科学0.0000.7100.18953 6.30.000810.184收录环境科学0.0000.7420.130100 3.00.000530.102收录环境科学0.0002.9140.444363.80.001370.674收录环境科学0.0000.6710.082494.70.000450.132收录环境科学中科院JCR 大类分区IF升降SCI收录本土期刊包含港澳Logo Here4上升保持收录3上升保持收录4上升保持收录4上升保持收录3下降保持收录4上升保持收录4下降保持收录4下降保持收录3持平保持收录3下降保持收录4上升保持收录4下降保持收录2下降保持收录4上升保持收录4上升保持收录4上升保持收录4上升保持收录4下降保持收录4下降保持收录2上升保持收录2上升保持收录3下降保持收录2下降保持收录2下降保持收录1下降保持收录3下降保持收录3上升保持收录4上升保持收录1上升保持收录4上升保持收录4上升保持收录3下降保持收录3下降保持收录4上升保持收录4下降保持收录2下降保持收录4下降保持收录4上升保持收录4下降保持收录4上升保持收录4下降保持收录4上升保持收录4下降保持收录4上升保持收录1下降保持收录4下降保持收录4下降保持收录3下降保持收录4下降保持收录4上升保持收录3下降保持收录2下降保持收录4上升保持收录4上升保持收录2下降保持收录3上升保持收录2上升保持收录3上升保持收录4下降保持收录1下降保持收录2下降保持收录4上升保持收录4持平保持收录3下降保持收录2上升保持收录4下降保持收录4上升保持收录2下降保持收录4上升保持收录3上升保持收录4上升保持收录4上升保持收录2下降保持收录4上升保持收录4下降保持收录3上升保持收录3上升保持收录4上升保持收录4上升保持收录1下降保持收录3上升保持收录4上升保持收录中国4上升保持收录中国4下降保持收录2下降保持收录2上升保持收录4上升保持收录3上升保持收录4上升保持收录4上升保持收录2上升保持收录1下降保持收录3上升保持收录4上升保持收录2上升保持收录4上升保持收录1上升保持收录4下降保持收录3上升保持收录4下降保持收录4上升保持收录中国3上升保持收录2下降保持收录4下降保持收录4下降保持收录3下降保持收录4下降保持收录。
international biodeterioration biodegradation评价
international biodeterioration biodegradation评价International Biodeterioration & Biodegradation是一本创刊于1992年的期刊,由ELSEVIER SCI LTD出版。
该期刊覆盖了生物- 环境科学的全领域,并且在该细分领域中具有较高的学术影响力和专业度认可。
由于对原创文章的创新性要求较高,如果文章质量很高,可以考虑投稿。
该期刊的平均审稿速度约为3.0个月,影响因子为4.8,近期没有被列入国际期刊预警名单,因此是一个值得尝试的期刊。
该期刊发表关于退化或退化的生物学原因的原创研究论文和评论,致力于发表经过严格同行评审的高质量原创文章,反映生物-环境科学领域的新进展、新技术、新成果,并促进该领域科研交流和科研成果转化。
scientia horticulturae 稿费
scientia horticulturae 稿费科学园艺学是研究植物种植和栽培的科学,它涉及到植物生长、繁殖、疾病防治、土壤改良、肥料使用等诸多方面。
《Scientia Horticulturae》是一本国际性的园艺学期刊,它旨在推动园艺学的研究和发展。
该期刊除了提供研究论文外,还包括综述文章、通讯、方法论和书评等内容。
本文将简要介绍该期刊的一些热门研究领域和相关内容。
1. 植物生长和发育:《Scientia Horticulturae》涵盖了不同植物种类的生长和发育过程的研究。
这些研究可能包括适应环境条件的变化、根系生长和发育的机制、植物开花和果实发育的调控等。
2. 繁殖和繁育:该期刊还关注园艺植物的繁殖和繁育方面的研究。
这些研究可能涉及到植物的传粉、传播和无性繁殖等方面,如果树嫁接技术、花卉无性繁殖技术等。
3. 植物疾病防治:园艺植物易受到病害的侵袭,因此疾病的防治和治疗也是该期刊关注的研究领域。
研究人员可能研究植物病原体的生物学、病害的诊断和检测方法、病害的防治策略等。
4. 土壤改良和肥料使用:植物在适宜的土壤条件下才能正常生长和发育。
因此,研究土壤改良和肥料使用的方法也是该期刊的关注重点。
这些研究可能包括土壤物理性质、土壤养分的供应和管理,以及有机肥料和肥料施用对植物生长和产量的影响等。
《Scientia Horticulturae》还涵盖其他一些关键研究领域,例如:节水技术、植物逆境抗性、果蔬贮藏和加工等。
该期刊的目的是促进园艺学的发展,为学术界和园艺行业提供有益的信息和研究成果。
对于作者来说,发表在《Scientia Horticulturae》上的稿费可能是一种奖励,是对其研究工作的认可和鼓励。
稿费的具体数额可能会根据研究论文的篇幅、质量和影响力等因素而有所不同。
总而言之,园艺学作为一门关于植物种植和栽培的科学,涵盖了植物生长和发育、繁殖和繁育、疾病防治、土壤改良和肥料使用等多个方面的研究内容。
引起花冠管长度演化的可能因子与例证
植物科学学报 2023,41(6):719~728Plant Science Journal DOI:10.11913/PSJ. 2095-0837. 23164童泽宇,黄双全. 引起花冠管长度演化的可能因子与例证[J]. 植物科学学报,2023,41(6):719−728Tong ZY,Huang SQ. Potential selection driving evolution of long corolla tubes and case studies[J]. Plant Science Journal,2023,41(6):719−728引起花冠管长度演化的可能因子与例证童泽宇,黄双全*(华中师范大学生命科学学院,进化与生态研究所,武汉 430079)摘 要:多样的花冠形态具有保护花内性器官、吸引传粉者等多种作用,管状花的出现被认为是花瓣演化的一个变革性事件。
自达尔文以来,学者们试图回答的一个经典问题是:长花冠管的演化是否由传粉者口器与花冠管之间长度的“军备竞赛”引起?人们已在长花冠管的多个类群中开展了实验,试图寻找交互选择引起的动植物之间协同演化的证据。
本文总结了影响植物花冠管长演化的生物因素和非生物因素,探讨了传粉者、盗蜜者、植食者、光照、水分等因素在花冠管长演化中扮演的角色,重点梳理了传粉者介导花冠管长演化的4个潜在路径,以期为进一步探究植物花冠管长度的演化提供新的思路和方向。
关键词:花冠管长;传粉者介导选择;花部特征演化;协同演化;植物-传粉者相互作用中图分类号:Q948 文献标识码:A 文章编号:2095-0837(2023)06-0719-10Potential selection driving evolution of long corolla tubesand case studiesTong Ze-Yu,Huang Shuang-Quan*(Institute of Evolution and Ecology, School of Life Sciences, Central China Normal University, Wuhan 430079, China)Abstract:Diverse corolla morphologies are thought to function in protecting sexual organs within flowers and attracting pollinators. The transition from open to tubular flowers is considered a key innovation in the evolu-tion of corollas. A classic question that scholars have tried to answer since Darwin is: was the evolution of long corolla tubes caused by an arms-race relationship between the proboscis of the pollinator and the length of the corolla tube? Phenotypic manipulations have been conducted across multiple species with long corolla tubes to elucidate the mechanisms underlying the co-evolution between flowers and their interacting animal agents, driven by reciprocal selection. In this review, we summarize both the biotic and abiotic factors affec-ting corolla tube length, including the effects of pollinators, nectar robbers, and herbivores, as well as environ-mental factors like light and water availability. Moreover, we propose four potential evolutionary pathways for the development of pollinator-mediated long-tubed corollas. This review aims to provide insights and gui-dance for future studies on the evolution of tubular flowers.Key words:Corolla tube length;Pollinator-mediated selection;Flora trait evolution;Coevolution;Plant-pollinator interaction收稿日期:2023-06-04,修回日期:2023-07-24。
豚草和三裂叶豚草不同植株部位种子萌发与入侵扩散关系
environments rapidly and expand their populations. However, the seeds produced in the middle and lower positions diffuse
around the parent plants, and their germination rates were low, which alleviated intraspecific competition. The
上基、中基、下中、下基的种子数占比约 27%,表明当年生产的种子有近 73% 的比例具有远距离扩散的潜力。 3) 豚草和三裂叶
豚草不同植株部位种子的萌发率具有上端>中端>下端的趋势;初始萌发时间为下端>中端>上端;萌发持续时间为上端>中端>
下端。 这种萌发方式避免了同一生长季大批种子同时萌发有可能导致高密度死亡的风险。 基于上述研究分析,认为豚草和三
two species in Xinyuan, it was considered that seed size is a factor affecting the regional differences in distribution of the
two species. 2) The number of seeds produced in positions Up Up ( UU) , Middle Up ( MU) and Up Middle ( UM) of A.
meant that 73% of the seeds produced in the year had the potential for long distance diffusion. 3) The germination rats of
Metaphor-A Tool for Writing
Metaphor-A Tool for Writing周自强【期刊名称】《海外英语(上)》【年(卷),期】2011(000)010【摘要】Figurative speeches, especially metaphors that prevail essays, novels, reports, poems, etc. give flavor, if not life, to writings, help express the inexpressible, bring the abstract concrete, make the tedious succinct, and illuminate the vague and the ambiguous bright and clear. It seems that metaphors to writing is what salt to cooking ─ and more. In this paper, we are attempting to drag it to a "down-to-earth" tool to be handy to common learners by quoting examples from various styles to show how it can be used to increase language power and how important it is for ordinary Chinese learners of English to get familiar with and learn to employ metaphors and other figurative speeches in writing. We should be able to use words in non-literal sense to lend force to an idea, to heighten effects, or to create atmosphere, so as to make our writings eligible at least for practical use and readable for an ordinary native speaker of English.【总页数】3页(P22-23,26)【作者】周自强【作者单位】河海大学常州校区外语部,江苏常州213022【正文语种】中文【中图分类】H314【相关文献】1.INTEGRATION OF TRADITIONAL CHINESE WRITING AND PROCESS WRITING APPROACHES IN TEACHING EFL WRITING [J], ;2.A Study of Appling Online Writing Website to English Writing Teaching:A Structured Critical Review and Evaluation of OWL Online Writing Lab [J], 李玉影;李灏;任宏伟3.The Effect of Reading-based Writing On Enhancement of Senior Three Students'Writing Ability [J], 李琴4.The Study of the Effect of "The Process Writing Based on Reading-writing Portfolios" on Students' Writing Ability [J], 张倩倩5.New Approach to Chinese Writing:an Exploratory Study of Writing Performance on Social Q&A Online Community [J], 朱琳因版权原因,仅展示原文概要,查看原文内容请购买。
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An Evolutionary Approach to Case Adaptation Andrés Gómez de Silva Garza and Mary Lou MaherAppears in:Case-Based Reasoning Research and Applications. Third International Conference on Case-Based Reasoning, ICCBR-99, Monastery Seeon, Munich, Germany, July 1999, Proceedings.©Springer-VerlagAn Evolutionary Approach to Case AdaptationAndrés Gómez de Silva Garza and Mary Lou MaherKey Centre of Design ComputingDepartment of Architectural and Design ScienceUniversity of Sydney NSW 2006AustraliaFAX: (+61-2) 9351-3031Phone: (+61-2) 9351-2053E-mail: {andres,mary}@.auAbstract. We present a case adaptation method that employs ideas from the field ofgenetic algorithms. Two types of adaptations, case combination and case mutation, areused to evolve variations on the contents of retrieved cases until a satisfactory solution isfound for a new specified problem. A solution is satisfactory if it matches the specifiedrequirements and does not violate any constraints imposed by the domain ofapplicability. We have implemented our ideas in a computational system calledGENCAD, applied to the layout design of residences such that they conform to theprinciples of feng shui, the Chinese art of placement. This implementation allows us toevaluate the use of GA’s for case adaptation in CBR. Experimental results show the roleof representation and constraints.1 IntroductionMany different methods have been proposed for performing the task of case adaptation in CBR. They have been surveyed in several publications, including [1], [2], and [3]. Different approaches may be better for different domains, different knowledge representation schemes, different reasoning tasks, or other reasons. Approaches may differ on the types of adaptation they support, the amount of change in a case they permit an adaptation to make, the number of cases they can rely on to generate solutions to new problems, and other factors. The adaptation method we present here is flexible, in that it allows for a wide variety of options along all of these dimensions. In our approach, several types of adaptation are available, cases may end up being completely transformed or just slightly tweaked, and final solutions may contain features from one or many cases.In this paper we present a case adaptation method based on genetic algorithms. In this method, cases are adapted incrementally and in parallel, until a satisfactory solution is found for a given problem. We have employed this approach for design, though it can be used forother reasoning tasks. Within design, we have tried it out on several domains, though in this paper we focus on just one, introduced below. The main concern of this paper is to describe our process model for case adaptation, not to discuss the quality of the designs produced by the application.Our case adaptation method supports two broad types of adaptation: parametric and structural. Parametric adaptation of cases is achieved through mutation. Structural adaptation of cases is achieved through crossover. Depending on the specifics of a given domain and the richness of the representation chosen for it, several mutation and crossover operators, with different nuances in the effects they produce, can potentially be made available.The method assumes that the requirements of a new problem will partially match, and therefore result in retrieving, more than one case in memory. These retrieved cases are used to seed an evolutionary process, i.e., they form its initial population. The adaptations produced by the crossover and mutation operators of the evolutionary process are evaluated, and the best ones selected to participate in the next round of genetic adaptations, until a satisfactory solution is found. Evaluation requires domain knowledge in order to recognise whether proposed solutions are acceptable for a given domain or not; crossover, mutation, and selection can operate independently of the domain.Depending on which randomly evolved variations on the originally retrieved cases are selected to remain in the population after being evaluated, final solutions may have evolved from just one of the cases, or from all of them. They may differ greatly in structure and/or in parameter values from all of the originally retrieved cases, or may be similar to one or several of them. Thus, the method is useful in a wide variety of problem situations and domains requiring different types and degrees of adaptation.In the following sections we discuss our evolutionary case adaptation method in more detail, we present an implementation for a specific domain and the knowledge representations we have adopted for this domain, and we give some experimental results.2 Case Adaptation MethodWe have developed a process model of design that combines the precedent-centered reasoning capabilities of case-based reasoning (CBR) (see for example [1]) with the incremental evolution of multiple potential solutions, an idea taken from the paradigm of genetic algorithms (GA’s) (see for example [4]). The process model involves the use of CBR as the overall reasoning strategy and the use of a GA to perform the case adaptation subtask. Because a general-purpose, knowledge-independent GA is used, case adaptation is knowledge-lean. It is only in the evaluation module of the GA that domain knowledge is required so that proper decisions are made about which potential solutions generated by the GA are useful to keep in future GA cycles.Our process model is shown in Fig. 1. In this model we assume the existence of a case memory in which descriptions of previously existing solutions are stored. Each case is represented as a set of attribute-value pairs. The cases that are retrieved from memory given a new problem specification are adapted by repeatedly combining and modifying their descriptive features. After each cycle of combination and modification, solutions are evaluated and the best are selected, to be adapted in the next cycle. Through this incremental, evolutionary process, the case adaptation method converges to a satisfactory solution to the new problem. The solution will contain features and/or modifications of features from several of the cases that were initially retrieved from memory. Thus, our process model adapts pastsolutions by evolving different combinations of their features in parallel and continuously, until a satisfactory combination is found.Fig. 1.Evolutionary case adaptation method.The main emphasis of our process model is on proposing new solutions based on the knowledge contained in previously known solutions, i.e., it is a precedent-based approach. But a major component is the evolutionary approach to adapting the known solutions in order to generate solutions to new problems. The two strategies of CBR and GA’s complement each other. The cases retrieved from memory serve as the initial population for a genetic algorithm, while the genetic algorithm adapts the cases until it finds an acceptable solution.The combination subtask of case adaptation performs several cut-and-paste crossover operations. Each crossover is done on two randomly-chosen “parents” from the population of potential solutions, at randomly-chosen crossover points, and produces two “offspring”suggested solutions. The modification subtask performs several mutation operations. Each mutation produces a new “offspring” suggested solution by:•randomly choosing a “parent” from the population of potential solutions,•randomly selecting an element to mutate in the description of the parent,•randomly choosing an attribute of that element to mutate, and•randomly selecting a new value for that attribute.Knowledge of which values are valid for which attributes can be used so that mutation does not suggest completely nonsensical solutions. If the process model were to be used to design buildings, for instance, it would be a waste of time for mutation to change the value of the number-of-stories attribute from 25 to 834 or –15, for instance.The evaluation subtask of case adaptation analyses a suggested solution according to domain constraints. Depending on the domain, different constraints may have to be satisfied in order for a solution to be considered acceptable or satisfactory. A fitness value is assignedduring evaluation to each suggested solution. The total fitness F of a given solution, given N constraints (C 1 through C N ) and M problem requirements (R 1 through R M ), is calculated with the following equation:N MF = Σ C i + Σ R ji=1 j=1where C i = 0 if constraint C i is not violated by the solution or C i = 1 if constraint C i is violated by the solution, and R j = 0 if requirement R j is met by the solution orR j = 1 if requirement R j is not met by the solution.Convergence to an acceptable solution occurs if an individual in the population has a total fitness of 0, meaning that none of the constraints has been violated and all of the problem requirements have been met.The selection subtask of case adaptation takes all of the evaluated individuals in a population of suggested solutions, including those inherited from previous adaptive cycles and those generated in the current one, and keeps the k best ones to serve as the initial population of the next cycle. The value of k , as well as the number of offspring produced at each cycle by crossover and mutation, is chosen so that the size of the population does not change from one cycle to the next. Thus, the value of k depends on the number of cases initially retrieved from memory.In this method of case adaptation, the synthesis of potential solutions is done in a task-and domain-independent fashion. The power of mutation can be enhanced by providing access to some simple domain knowledge, namely the values that are valid for the attributes that describe objects in the domain, as mentioned above. But on the whole, domain knowledge is needed only for evaluating the generated solutions to determine their quality. In other words, recognition (analytical) knowledge, rather than generative knowledge, is needed to apply our method to a given domain.3 Implementation and DomainWe have implemented our ideas in a computational system named GENCAD written in Common LISP. Our method of case adaptation has been applied to the structural engineering design of high-rise buildings [5] and to the layout design of residences such that they conform to the principles of feng shui (pronounced “fong sway”), the Chinese art of placement. Here we describe the feng shui application.Feng shui, also known as Chinese geomancy, is an ancient technique that, among other things, determines the quality of proposed or existing layouts of residences according to several rules of thumb. Some of these heuristics seem to have a basis in common sense, or in a psychological or sociological appreciation of the human beings that inhabit (or intend to inhabit) the residence. Other heuristics seem to be of a more superstitious nature.There are several different feng shui sects that may contradict each other or place different priorities on different aspects of residential layouts. Despite this variety, of prime importance to performing any feng shui analysis is information on the relative positions of objects. In addition, other attributes of objects are usually also taken into account, such astheir orientations, shapes, and relative sizes. In our work we have used the knowledge of feng shui presented in [6], which corresponds to the Tibetan black-hat sect of feng shui.Feng shui analyses different aspects of a residential layout to determine its auspiciousness or lack thereof. Some classes of inauspicious layouts can be “cured” by the proper placement of an acceptable curing object. Thus, feng shui knowledge is complex, in that some potentially bad layouts can actually be acceptable if the proper cure is present. It is not just a matter of determining whether a layout is “good” or “bad,” but even if it would normally be considered bad, one has to determine whether it has been cured or not before rejecting it outright.The feng shui knowledge contained in [6] applies to three different levels of description of a residence:•The landscape level (the location of a residence with respect to other objects in its environment such as mountains, rivers, roads, etc.),•The house level (the relative placement of the rooms and functional spaces within a residence, such as bedrooms and bathrooms, as well as the connections betweenthem, such as doors and windows), and•The room level (the location of furniture, decorations, and other objects within each room or functional space in a residence).GENCAD applies its case adaptation GA to one of the three levels of description of a residence at a time. This is because there are very few feng shui constraints that relate objects belonging to different levels of description; the constraints involve relations between objects within the same level. Thus, potential solutions to the new problem at the landscape level can be evolved (and evaluated) independently from potential solutions to the same new problem at the house level, etc. For other domains, GENCAD’s GA might have to operate on and evolve hierarchical solutions containing several levels of description at once. This will have implications for the speed of convergence as well as the complexity of the implementation of the crossover and mutation operators.4 Knowledge RepresentationFeng shui analysis assumes knowledge of spatial relationships among the objects at the different levels. Absolute locations and exact measures of distances and other geometric quantities are not as important. Because of this, a qualitative spatial representation has been chosen to describe the locations of objects within each of the three levels. We locate objects on each level in a 3x3 spatial grid, with each sector within the grid assigned a unique number between 1 and 9 to identify it. The grid is shown as follows, with north assumed to be at the top of the page:123456789Objects can occupy more than one grid sector, and grid sectors can contain more than one object, making the representation flexible. The resolution of this representation is not high, but considering the qualitative nature of a typical feng shui analysis and the number of objects that typically need to be represented at each of the three levels, it is adequate in most cases.4.1 Case RepresentationGENCAD’s case library currently contains 12 cases, each of which describes one of Frank Lloyd Wright’s prairie houses, obtained from [7]. Note that the designs of these houses do not necessarily conform to the principles of feng shui. However, designs that are acceptable to feng shui practitioners can still be generated by evolving combinations and mutations of the features of the design cases. If the original cases did conform to feng shui practice, given a new problem, convergence to a solution acceptable to feng shui practitioners might be faster, but this is not a requirement of our case adaptation method.Each of GENCAD’s design cases is a residence described at the landscape, house, and room levels. Within each level, objects are represented using attribute-value pairs to describe features that are relevant to feng shui analysis. Some attributes such as locations and types of objects are required for all objects, whereas others such as shapes and steepness are optional, and don’t even make sense for some objects. A diagrammatic example of a residence at the landscape level is shown in Fig. 2. This is followed by an abbreviated version of the symbolic case representation of the same residence.Figure 2. A residence and its place in the landscape.(((level landscape)(elements (((type mountain) (name dragon-mountain)(location (1 2 4)) (steepness high) ...)((type pond) (name fish-pond) (location (6)) (clarity murky) ...)((type house) (name my-house) (location (5))) ...)))...)When running GENCAD at the landscape level, this is the fragment of a case that would form part of the population of the GA. The fragments describing the house and room levels would be dealt with separately. The list of attribute-value pairs is modified through mutation and combined with that of other cases through crossover as the GA proceeds.4.2 Representation of Feng Shui Analysis KnowledgeFeng shui analysis knowledge is used in the evaluation function of the GA. We have taken the text description of the analysis knowledge and converted it to a set of constraints; each constraint is implemented as a procedure. There are several constraints at each of the three levels of feng shui description.An example of a feng shui constraint at the landscape level, quoted directly from [6], is:A house facing a hill will be bad...CURE: If a house facesa mountain and the backyard is a garden, place a spotlightin the back of the garden and shine it toward the top of the house, or install a flagpole at the rear of the garden to balance ch’i. [Page 35]This constraint is implemented by first finding the description of all the houses and mountains/hills at the landscape level, particularly their locations and the orientations of the houses (if known). A predicate facing has been written that, given the location and orientation of an object, and the location of a second object (within the 3x3 grid), determines whether or not the first object faces the second (even partially). If any of the houses is located and oriented such that it faces any of the mountains/hills in the landscape, then the constraint has been violated. However, first we must check whether or not a cure is present for the constraint violation, i.e., if there is a garden behind the violating house, and if so whether there is a flagpole in it, or a spotlight oriented towards the house. A predicate behind has been written that, given the location of an object, and the location and orientation of a second object, determines whether or not the first object is behind the second. The pseudocode that performs this analysis, i.e., the procedural representation of the constraint, given a proposed solution at the landscape level S, is shown as follows:Get the list H of all houses in S;Get the list M of all mountains/hills in S;Get the list C of all potential cures for this constraint in S;For each house h in H or until a bad omen has been found: Get the location lh of h;Get the orientation oh of h;For each mountain/hill m in M or until a bad omen hasbeen found:Get the location lm of m;If facing(lh,oh,lm) Then:Get the list G of all gardens in S;Set flag g-behind? to False;RepeatGet the next unprocessed garden g in G;Get the location lg of g;If behind(lg,lh,oh) ThenSet flag g-behind? to True;Until g-behind?=True or all gardens in G haveBeen processed;If g-behind?=True ThenFor each potential cure c in C or until a bad omen has been found:Get the location lc of c;Get the type tc of c;If tc=spotlight Then:Get the orientation oc of c;If facing(lc,oc,lh) and subset(lc,lg)Then signal a bad omen situation;ElseIf subset(lc,lg)Then signal a bad omen situation;5 Evaluation and Experimental ResultsIn this section we evaluate our evolutionary case adaptation method according to three issues: the coverage of the method, its efficiency, and the quality of the solutions it produces.5.1 CoverageOften, CBR is criticised because even large case bases are not guaranteed to cover the entire search space, thus making some problems unsolvable using “pure” CBR. In our framework, even small case bases can provide sufficient information on typical structures and contents of solutions to problems in the domain for the method to eventually converge to a solution. Of course, the larger the case base, the more cases are likely to be retrieved given a new set of problem requirements, and the faster the GA is likely to find a satisfactory adaptation of their features and converge.If N cases are initially contained in the population of the GA, then after 1 cycle of the GA the proposed solutions in its population will combine features from at most 2 cases (due to crossover). Thus, after N-1 cycles some of the proposed solutions in the population can combine features from all of the N retrieved cases. The selection operator in the GA ensures that only those combinations that seem to be leading towards an acceptable solution are kept for future GA cycles, i.e., it helps to prune the search.But even an exhaustive search of all the possible combinations of the features of all retrieved cases is not guaranteed to find satisfactory solutions to the new problem. The inclusion of a mutation operator in the GA, in addition to combination, ensures that all points in the search space can potentially be reached. Of course, whether a certain point will be reached or not depends on the particular sequence of mutations and combinations followed during a given application of the GA to the retrieved cases. The mutation operator introduces into the proposed solutions features that weren’t present in any of the originally retrieved cases, or different values for those features that were present. Thus, our method can potentially cover the entire search space, even if a large case base is not available.5.2 EfficiencyWe have explored the efficiency of combining GA’s with CBR by comparing our method with a GA that is exactly the same except for the lack of cases. In the alternative method, instead of initiating the GA search with a population consisting of cases retrieved from memory, we initiated it with randomly generated “cases” (i.e., random starting points in the search space). In this way, any differences in efficiency will be attributable to the use of CBR as the guiding framework, and we can evaluate our decision to combine the two AI paradigms of CBR and GA’s.In order to perform this efficiency experiment, GENCAD was run 20 times using 12 cases retrieved from a case base of floor plans of Frank Lloyd Wright prairie houses, and 20 times using 12 randomly-generated cases, on the same problem. The problem specification for this test problem (at the landscape level) is:(((level landscape)(requirements ((house 1) (river 1) (trees 2)))))This problem specification can be interpreted as “we want to build a house on a property in which there is a river, and we’re thinking of planting two clumps of trees around the house.” The problem is now to use GENCAD to generate a configuration containing these four elements, specifying their relative positions within the landscape, such that the configuration is auspicious according to the principles of feng shui.GENCAD was given a limit of 500 GA cycles in which to find an acceptable solution, i.e., if convergence did not occur by cycle 500, the search was ended without a solution being given. Some of the cases in the randomly generated case base, as well as the Frank Lloyd Wright cases, do contain two clumps of trees, and/or a house, and/or a river in the landscape. In addition, there are configurations of these four types of element that are valid according to feng shui practice. Therefore, achieving a solution through the cyclical combination and/or mutation of the cases retrieved from either case base is theoretically possible.In the experiment, 5 of the 20 trials using the random starting points converged. Similarly, 5 of the 20 trials using the Frank Lloyd Wright cases converged. Thus, whether cases or random starting points are used to initiate the search doesn’t seem to make a difference as far as the frequency of convergence. However, a clear difference can be seen when we analyse the number of GA cycles required before convergence occurred (in those trials in which it did occur), as seen in Table 1.Table 1. GA cycles required before convergence:Trial #Random Trial #FLW cases11142554933331341135736321427437406171603990Avg.:241.6Avg.:123.2As can be seen from the results, when cases are used to guide (i.e., provide starting points for) the search, convergence occurs on average twice as fast as when the search is initiated from random starting points. This demonstrates the efficiency of combining the ideas of CBR with those from GA’s. Convergence does not always occur, as can also be seen (or does not occur within a reasonable number of iterations). Whether it will converge or not, or how rapidly it will converge, can vary greatly due to the random nature of the genetic operators of crossover and mutation. However, the process can be applied again and again to the same problem, using the same initial set of retrieved cases, and it is possible that it will converge in future attempts.5.3 QualityThe use of CBR as the overall framework helps ensure that the solutions proposed by our method are of high quality. For example, a typical problem specification for a floor plan layout at the house level is that the house should have 3 bedrooms and 2 bathrooms. A residence of this size typically also has, as a minimum, a kitchen, a living room, and a dining room. These are not normally given as requirements, but it is an implicit assumption that any solution will have these additional rooms.Now let us assume that we used the problem specification mentioned in the last paragraph to perform a GA search using randomly generated initial solutions, or to perform an exhaustive search of the solution space, for instance. Such searches would most probably eventually find a solution that has 3 bedrooms and 2 bathrooms, and that satisfies any domain constraints (such as relationships among the rooms acceptable to feng shui practitioners). But it would be likely that these would be the only components that would be present in the solution. Unless further knowledge and heuristics were used to guide the search, solutions would be minimalistic.Instead, by using cases that include kitchens, living rooms, and dining rooms (and perhaps additional rooms that might be considered to be useful post facto such as pantries) to initiate the search, the solutions to which our method will converge will most likely also include these important but unspecified rooms. Thus, the quality of solutions proposed by our method is equal or greater than if CBR were not used as the guiding framework. Cases provide complete scenarios that serve to guide both the structure and contents of proposed solutions.6 DiscussionWe have presented a case adaptation method that is based on ideas from genetic algorithms. Variations on retrieved cases are evolved incrementally, and at each cycle their quality is verified and the best variants from amongst the initial population plus the new variants generated at the current cycle are kept. This evolutionary method of case adaptation combines the benefits of case-based reasoning and other knowledge-based approaches with those of general-purpose problem solvers such as genetic algorithms.For instance, being able to use starting points for problem solving search based on similar past experiences, and being able to apply the process model to highly-specialised problem solving domains are two advantages of CBR. On the other hand, having a large number of operators with greatly differing effects available, and being able to apply the process model to a wide variety of problem solving domains are two advantages of GA’s. Our evolutionary method of case adaptation benefits from having all of these characteristics.Domain knowledge is required and represented in the form of constraints used for the evaluation of proposed solutions; this is recognition knowledge, not generative knowledge. This difference with other approaches is especially important in applying our method to tasks such as design. In design it is relatively easy to recognise whether a proposed design is an acceptable solution for a given problem or not, whereas it is quite difficult to come up with a set of reasoning steps or heuristics to follow that will lead to the generation of acceptable designs. The knowledge engineer’s task of knowledge elicitation and knowledge acquisition is thus simplified when using our evolutionary approach to case adaptation.This use of constraints for evaluation rather than generation is one of the differences between our work and that of others that have used constraint-satisfation techniques in the context of CBR, for instance [8], [9], [10], or [11]. In these projects, constraints with potentially complex interactions guide the generation of solutions to new problems by adapting past cases. This generation of solutions uses domain knowledge or heuristics to make what is generally an NP-complete problem tractable. In our method, the constraints are independent of each other, and they help in a cumulative fashion to eliminate bad solutions, rather than in a mutually interacting way to generate good ones.There has been other work in the past that has combined concepts from GA’s with CBR. [12] presents a GA that is initialised based on the information held in cases. However, in [12] cases contain descriptions of past executions of a GA (e.g., the values of the GA parameters, the task environment in which those parameter values were used successfully, etc.), irrespective of the type of problem being solved with the GA. Thus, cases help the GA dynamically adapt to changing problem situations; the authors use concepts from CBR in aid of GA’s. In our work, on the other hand, cases contain descriptions of known solutions for the type of problem being solved, and these cases provide guidance for the search that our case adaptation GA will perform; thus, we use concepts from GA’s in aid of CBR.The research presented in [13] is more similar to ours, in that cases contain descriptions of solutions to the type of problem being solved, and a GA is used to adapt the cases to solve the problem. However, [13] is not a pure CBR approach, as only a small fraction (10%-15%) of the initial population in the GA comes from cases in memory; most of the initial population is generated at random, as in a classical GA. The authors do this for valid reasons of balancing exploration and exploitation in their GA search, but it provides a different flavour to their research. Again, their work places more of an emphasis on the GA, and on making it efficient and effective, than on contributing to CBR research. In contrast, we have examined the possibilities of using a GA for case adaptation from the perspective of CBR. References1.Kolodner, J.L.: Case-Based Reasoning, Morgan Kaufmann Publishers (1993)2.Leake, D.B.: Case-Based Reasoning: Experiences, Lessons, & Future Directions, AAAI Press/TheMIT Press, Boston (1996)3.Maher, M.L. and Pu, P. (eds.): Issues and Applications of Case-Based Reasoning in Design,Lawrence Erlbaum Associates, Mahwah, New Jersey (1997)4.Mitchell, M.: An Introduction to Genetic Algorithms (Complex Adaptive Systems Series), MITPress, Boston (1998)5.Gómez de Silva Garza, A. and Maher, M.L.: A Knowledge-Lean Structural Engineering DesignExpert System, Proceedings of the Fourth World Congress on Expert Systems, Mexico City, Mexico (1998)6.Rossbach, S.: Interior Design with Feng Shui, Rider Books, London (1987)。