Unruh quantization in presence of a condensate
DNA的复制课件-2023-2024学年高一下学期生物人教版(2019)必修2
C.这M个子代T₂噬菌体中,含32P的T₂噬菌体所占的比例为1/M
有2个,故其所占的比例为2/M,C 错误。培养足够长的时间,会出现 不含32P而含35S的T₂噬菌体,但一般不会出现含32P的大肠杆菌,D 错 误。
3 、在一个密闭的容器里,用含有同位素13C的脱氧核苷酸合成一个 DNA分子,然后加入普通的含12C的脱氧核苷酸,经n次复制后,所得DNA 分子中含¹2C的脱氧核苷酸链数与含13C的脱氧核苷酸链数之比是
特点 半保留复制;边解旋边复制;多起点复制;双向复制
结果 子链与母链结合,构成两个相同的新的DNA分子
意义 保持了遗传信息的连续性
总结规律
规律1:若 一 个DNA 复制n次。
1.在子代中共形成 2n 个DNA, 其中含有亲代
DNA链的DNA分子数 2 0 2.含有亲代DNA链的DNA分子数占DNA分子总
D . 含 1 0 0 个 碱 基 对 ( 其 中 胞 嘧 啶 6 0 个 ) 的 DNA复 制 3 次 共 需 要 2 8 0 个
2、用 DNA双链均被32P标记的一个T,噬菌体侵染被35S标记的大肠杆菌, 一段时间后释放出出了M个子代T₂噬菌体。下列有关叙述正确的是
()
A.用32P标记T₂噬菌体的方法与用35S标记大肠杆菌的方法相同
It has not eseaped our notice that the specife pairing we have postulated immediately guggests a p⁰ssible copying mechanism for the genetic materiaI.
Full details of the structure,including the conditions assumed in building it,together with a set of co-ordinatos for the atoms,will be published elsewhere.
英语作文推荐书店的书
When recommending books at a bookstore, its essential to consider the interests and preferences of the reader. Here are a few suggestions spanning various genres that are sure to cater to a diverse audience:1. Fiction: For those who enjoy a good story, To Kill a Mockingbird by Harper Lee is a timeless classic that explores themes of racial injustice and moral growth. Its rich narrative and memorable characters make it a mustread.2. Science Fiction: Fans of the genre might appreciate Dune by Frank Herbert. This epic tale of political intrigue, environmental challenges, and human evolution is a masterpiece that has captivated readers for decades.3. Mystery/Thriller: For those who cant put down a pageturner, Gone Girl by Gillian Flynn offers a gripping story of deceit, obsession, and the dark side of relationships.4. NonFiction: Sapiens: A Brief History of Humankind by Yuval Noah Harari provides an engaging and thoughtprovoking exploration of the history of our species, from the emergence of Homo sapiens in Africa to the present day.5. Biography/Autobiography: Becoming by Michelle Obama is an inspiring memoir that offers insight into the life of the former First Lady, her journey, and her reflections on the experiences that have shaped her.6. Selfhelp: The 7 Habits of Highly Effective People by Stephen R. Covey is a perennial favorite that offers practical advice on personal development and effectiveness.7. Young Adult: The Hunger Games by Suzanne Collins is a dystopian novel that has resonated with young readers for its themes of survival, rebellion, and the human spirit.8. Fantasy: Harry Potter series by J.K. Rowling is a staple for fantasy lovers, offering a magical world of witches and wizards that has captured the imagination of readers of all ages.9. Historical Fiction: All the Light We Cannot See by Anthony Doerr is a beautifully written novel that tells the parallel stories of a blind French girl and a German boy during World War II.10. Poetry: For those who appreciate the power of verse, The Waste Land by T.S. Eliot isa modernist masterpiece that explores themes of disillusionment and cultural collapse.When recommending books, its also important to consider the readers current mood or what they hope to gain from their reading experience. Whether theyre looking for escapism, knowledge, or inspiration, theres a book out there to suit every need.。
2024年同等学力申硕英语考试真题
2024年同等学力申硕英语考试真题2024 National Postgraduate Entrance Examination (English)Part I Listening Comprehension (20 points)Section ADirections: In this section, you will hear 10 short conversations. At the end of each conversation, a question will be asked about what was said. Both the conversation and the question will be spoken only once. After each question, there will be a pause. During the pause, you must read the four choices marked A), B), C) and D), and decide which is the best answer. Then mark the corresponding letter on Answer Sheet 1 with a single line through the center.1. A) At a news conference. B) In a classroom. C) At a restaurant. D) In a library.2. A) She is sick. B) She is busy. C) She is on vacation. D) She is changing jobs.3. A) The man should have told her. B) She is surprised at the price. C) The man should discuss it with her. D) She agrees with the man.4. A) He will ask his friend for help. B) He will fix it himself. C) He knows nothing about it. D) He will hire someone to do it.5. A) She wants to be excused from going. B) She plans to go.C) She has an important event that night. D) She doesn't know if she'll be able to make it.6. A) They should have partied more. B) She should eat more fruit. C) They should hurry to get to the beach on time. D) She should get ready faster.7. A) The man is almost out of gas. B) The woman is out of gas. C) The man is almost at a gas station. D) The man needs to buy gas before he leaves.8. A) Purple. B) White. C) Black. D) Green.9. A) Guitar lessons. B) Drawing lessons. C) Piano lessons. D) Dance lessons.10. A) Go to a concert. B) Prepare for a test. C) Play chess. D) Attend a play.Section BDirections: In this section, you will hear 3 short passages. At the end of each passage, you will hear some questions. Both thepassage and the questions will be spoken only once. After you hear a question, you must choose the best answer from the four choices marked A), B), C), and D). Then mark the corresponding letter on Answer Sheet 1 with a single line through the center.Passage One11.What induces people to give more strength to their satisfaction when writing an online journal entry than they do in their oral communication?A) The fact that in written communication mistakes can be corrected easily.B) The fact that journal writing is an individual activity.C) The fact that the written record can be later reviewed.D) The fact that journal writing is less time-consuming than oral communication.12. What shows advantages of blog as a form of communication?A) It makes interpersonal relationships easier to maintain.B) It attracts more readers than traditional diary writing.C) It allows readers to make anonymous comments.D) It provides more space for longer messages.Passage TwoQuestions 13 to 15 are based on the passage you have just heard.13.What does the speaker think of online learning?A) It lacks practical experiences. B) It is suitable for everyone.C) It provides more chances for interaction. D) It is more suitable for working professionals.14. Who are more likely to choose online learning?A) Unemployed people. B) Those with limited access to traditional education.C) College students. D) Working professionals.15. What is the advantage of an online classroom?A) It allows students to learn at their own pace.B) It provides more opportunity for face-to-face interaction.C) It offers students the experience of living on campus.D) It helps students develop lifelong friendships.Passage ThreeQuestions 16 to 20 are based on the passage you have just heard.16. Why is it difficult for researchers to study sleep's impact on health?A) Research subjects often fake their sleep patterns.B) Sleep patterns vary greatly from person to person.C) Technologies are not advanced enough to record sleep patterns.D) It is difficult to recruit sufficient volunteers.17. What is proved by the findings of the study?A) Inconsistent sleep patterns lead to health problems.B) Poor sleep quality causes mental disorders.C) High-quality sleep helps reduce obesity.D) A relaxing bedtime routine is essential for good sleep.18. What is the speaker's advice for a restful night's sleep?A) Keep irregular sleep habits. B) Drink coffee before going to bed.C) Establish a calming bedtime routine. D) Avoid relaxing activities before bedtime.19. Why do some people refuse to take the study results to heart?A) They are too busy to follow the advice. B) They are skeptical about the researchers.C) They think too much sleep is a waste of time. D) They doubt the accuracy of the study results.20. Why is it important for people to monitor their sleep habits?A) To ensure a healthy lifestyle. B) To please the researchers.C) To participate in a follow-up study. D) To relax their minds.Part II Reading Comprehension (40 points)Section ADirections: In this section, there are four passages followed by questions or incomplete statements. For each of them, there are four choices marked A), B), C), and D). Choose the one that best completes the statement or answers the question. Thenmark the corresponding letter on Answer Sheet 1 with a single line through the center.Passage OneQuestions 21 to 25 are based on the following passage.Noam Chomsky, the grandson of Jewish immigrants from Eastern Europe, was born in Philadelphia on December 7, 1928. He publicly opposed the Vietnam War in the 1960s and was arrested in 1967 for trying to disrupt military research at the Pentagon. Chomsky is well known for his profound impact on the philosophical and scientific understanding of human language.His major work Syntactic Structures and later The Chomsky Hierarchy have had a profound impact on linguistics, cognitive psychology, and the philosophy of mind. Chomsky's views on language acquisition revolutionized the field of linguistics. He argued that children are born with an innate ability to learn language and that the process of language acquisition ishard-wired into the human brain.21. What was Noam Chomsky's impact on the field of linguistics?A) He developed a new language. B) He published Syntactic Structures.C) He revolutionized language acquisition. D) He founded the Chomsky Hierarchy.22. Why was Chomsky arrested in 1967?A) For disrupting military research. B) For opposing the Vietnam War.C) For advocating human language. D) For his views on cognitive psychology.23. According to Chomsky, how does language acquisition occur?A) Through hard work. B) Through environmental exposure.C) Through cultural immersion. D) Through innate ability.24. What did Chomsky argue about children and language acquisition?A) They need formal education. B) They are hard-wired to learn language.C) They learn language from their parents. D) They need to be exposed to multiple languages.25. What are the subjects of Chomsky's major works?A) Cognitive psychology. B) Philosophy of mind.C) Military research. D) Language and linguistics.Passage TwoQuestions 26 to 30 are based on the following passage.Creating a positive classroom environment is crucial for student success and engagement. An environment that promotes trust, safety, and respect can enhance student motivation and learning outcomes. Here are some key factors that contribute to a positive classroom atmosphere:Clear expectations and guidelines that are consistently enforced; A supportive and inclusive classroom culture that values diversity; Opportunities for student participation and collaboration; Effective communication between teachers and students; Encouragement of critical thinking skills and creativity.26. Why is a positive classroom environment important?A) It enhances student motivation. B) It enforces clear guidelines.C) It creates a competitive atmosphere. D) It limits student participation.27. What contributes to a positive classroom atmosphere?A) Consistent enforcement of rules. B) A competitive culture.C) A lack of diversity. D) A strict teacher-student relationship.28. What kind of classroom culture values diversity?A) A conservative culture. B) An inclusive culture.C) A hierarchical culture. D) A singular culture.29. How can teachers promote a positive classroom atmosphere?A) Through limited communication. B) By discouraging critical thinking.C) By encouraging creativity. D) Through strict enforcement of guidelines.30. What does a positive classroom environment promote?A) Student engagement. B) Student competition.C) Teacher control. D) Limited participation.Passage ThreeQuestions 31 to 35 are based on the following passage.The concept of emotional intelligence (EI) has gained significant attention in recent years. Research shows that individuals with high EI are more likely to succeed in various aspects of life, including personal relationships, academic achievement, and career success. Developing emotional intelligence is essential for effective communication, conflict resolution, empathy, and self-awareness.EI consists of five key components: self-awareness,self-regulation, motivation, empathy, and social skills. By understanding and enhancing these components, individuals can improve their emotional intelligence and overall well-being.31. What is emotional intelligence (EI)?A) An academic achievement. B) A career success factor.C) An aspect of personal relationships. D) A concept of recent attention.32. What does research show about individuals with high EI?A) They are less likely to succeed in life. B) They lackself-awareness.C) They are more likely to succeed in various aspects of life.D) They are not motivated.33. Why is developing EI essential?A) For academic achievement. B) For conflict resolution.C) For career success. D) For self-improvement.34. What are the five key components of EI?A) Self-regulation only. B) Self-awareness, self-regulation, and empathy.C) Self-awareness, self-regulation, motivation, empathy, and social skills. D) Self-awareness and self-regulation only.35. How can individuals improve their emotional intelligence?A) By avoiding social interactions. B) By understanding and enhancing key components.C) By focusing solely on career success. D) By ignoringself-awareness.Passage FourQuestions 36 to 40 are based on the following passage.Nature plays a significant role in human health andwell-being. Research shows that spending time in nature can reduce stress, improve mood, and enhance overall mental health.Nature therapy, also known as ecotherapy, has gained popularity as a form of treatment for various mental health conditions.There are many ways to incorporate nature therapy into daily life, such as spending time in natural settings like parks, forests, or gardens; engaging in outdoor activities like hiking, gardening, or bird-watching; practicing mindfulness and meditation in nature; and disconnecting from technology and reconnecting with the natural world.36. What role does nature play in human health andwell-being?A) A minor role. B) No role. C) A significant role. D) An insignificant role.37. What does research show about spending time in nature?A) It increases stress. B) It decreases mood.C) It enhances overall mental health. D) It limits mental health.38. What has gained popularity as a form of treatment for mental health conditions?A) Meditation. B) Yoga. C) Nature therapy. D) Ecotherapy.39. How can nature therapy be incorporated into daily life?A) By spending time indoors. B) By avoiding outdoor activities.C) By practicing mindfulness indoors. D) By engaging in outdoor activities.40. How can individuals reconnect with nature?A) By spending more time with technology. B) By avoiding natural settings.C) By disconnecting from technology. D) By practicing mindfulness indoors.Section BDirections: In this section, you will read 2 passages and answer some questions or complete statements. Use the passage to answer the questions or complete the statements. Then mark the corresponding letter on Answer Sheet 1 with a single line through the center.Passage OneQuestions 41 to 45 are based on the following passage.The importance of multicultural education in today's diverse world cannot be overstated. Multicultural education aims to promote diversity, equity, and inclusion in the classroom and beyond. It acknowledges and celebrates different cultural backgrounds, languages, traditions, and perspectives, fostering a sense of belonging and respect among students.By incorporating multicultural education into the curriculum, teachers can provide students with a more holistic and inclusive learning experience. This approach helps students develop a deeper understanding and appreciation of diverse cultures, strengthening their empathy, communication skills, and critical thinking abilities.41. What is the aim of multicultural education?A) To limit diversity. B) To promote diversity, equity, and inclusion.C) To restrict cultural backgrounds. D) To isolate students.42. What does multicultural education acknowledge and celebrate?A) Different languages only. B) Different cultural backgrounds, languages, traditions, and perspectives.C) Tradition only. D) Perspectives only.43. How does multicultural education benefit students?A) By limiting their understanding. B) By limiting their perspective.C) By providing a holistic and inclusive learning experience.D) By isolating them from diverse cultures.44. How does multicultural education strengthen students' empathy?A) By limiting their cultural experiences. B) By limiting their communication skills.C) By providing a deeper understanding and appreciation of diverse cultures. D) By isolating them from diversity.45. What are the outcomes of incorporating multicultural education into the curriculum?A) Strengthening students' limitations. B) Strengthening students' empathy.C) Limiting students' critical thinking abilities. D) Limiting students' communication skills.Passage TwoQuestions 46 to 50 are based on the following passage.Climate change is one of the most pressing global challenges of our time. It poses significant threats to the environment, human health, and the economy. To address these challenges, individuals, communities, governments, and businesses must take action to reduce greenhouse gas emissions, transition to renewable energy sources, and promote sustainable practices.Climate change impacts various aspects of life, including weather patterns, sea levels, biodiversity, and food security. By taking proactive measures to mitigate the effects of climate change, we can create a more sustainable and resilient future for generations to come.46. What is one of the most pressing global challenges of our time?A) Climate change. B) Water crisis. C) Pollution. D) Overpopulation.47. How does climate change impact the environment?A) Positively. B) Negatively. C) Minimally.48. What must individuals, communities, governments, and businesses do to address climate change?A) Nothing. B) Take action to reduce greenhouse gas emissions. C) Continue current practices.49. What aspects of life does climate change impact?A) Weather patterns. B) Financial markets. C) Biodiversity.50. How can proactive measures mitigate the effects of climate change?A) By increasing greenhouse gas emissions. B) By transitioning to non-renewable energy sources. C) By creating a sustainable and resilient future.Part III Vocabulary and Structure (20 points)Directions: There are 30 incomplete sentences in this part. For each sentence, there are four choices marked A), B), C), and D). Choose the one that best completes the sentence. Then mark the corresponding letter on Answer Sheet 1 with a single line through the center.51. Relying solely on social media for news updates could lead to _____ misinformation.A) an increase in B) a surge of C) an influx of D) a proliferation of52. The new company policy requires employees to _____ their work before submitting it to their supervisor.A) edit B) revised C) subvert D) concoct53. The conference committee ____ to hold the event outdoors due to favorable weather conditions.A) determined B) highlighted C) faltered D) limited54. The management team decided to ____ the proposal after considering the potential risks.A) scour B) override C) align D) endorse55. The construction of the new subway line has been ____ delayed due to unexpected funding issues.A) harshly B) promptly C) visibly D) significantly56. The students were _____ for their hard work and dedication to the project.A) criticized B) invalidated C) recognized D) overlooked57. The _____ of plastic bags has been banned in many countries to reduce pollution.A) discretion B) proliferation C) confiscation D) utilization58. The team must _____ their strategies to adapt to the changing market conditions.A) appeal B) dedicate C) modify D) fluctuate59. The home renovation project was _____ due to the contractor's failure to secure the necessary permits.A) compromised B) endorsed C) validated D) optimized60. The new software program allows users to _____ their files securely on the cloud.A) endorse B) retrieve C) utilize D) redeemPart IV Reading Comprehension (20 points)Section ADirections: In this section, there is a passage with ten blanks. You are required to select one word for each blank from a list of choices given in a word bank following the passage. Read the passage through carefully before making your choices. Each choice in the word bank is identified by a letter. Please mark the corresponding letter for each item on Answer Sheet 2 with a single line through the center.PassageThe global economy is facing unprecedented challenges due to the ongoing COVID-19 pandemic. The (61) ____ of the virus has disrupted supply chains, halted international trade, and caused widespread economic uncertainty. As countries (62) ____ with the impact of the pandemic, policymakers are (63) ____ to implement measures to mitigate the economic fallout and support struggling industries. The resilience and adaptability of the global economy will be tested as nations work together to (64) ____ from the crisis and build a more sustainable future. It is essential for governments, businesses, and individuals to collaborate and (65) ____ innovative solutions to navigate the challenges ahead.Word Bank:A) outbreakB) recoverC) collaborateD) grappleE) implementSection BDirections: In this section, you are going to read a passage with ten statements attached to it. Each statement contains information given in one of the paragraphs. Identify the paragraph from which the information is derived. You may choose a paragraph more than once. Each paragraph is marked with a letter. Answer the questions by marking the corresponding letter on Answer Sheet 2 with a single line through the center.Passage(66) ____A) Multicultural education aims to promote diversity, equity, and inclusion in the classroom and beyond. It acknowledges and celebrates different cultural backgrounds, languages, traditions, and perspectives, fostering a sense of belonging and respect among students. By incorporating multicultural education into the curriculum, teachers can provide students with a more holistic and inclusive learning experience.B) Climate change is one of the most pressing global challenges of our time. It poses significant threats to the environment, human health, and the economy. To address these challenges, individuals, communities, governments, andbusinesses must take action to reduce greenhouse gas emissions, transition to renewable energy sources, and promote。
奈奎斯特定理的英文
奈奎斯特定理的英文The Nyquist Theorem, also known as the Nyquist Sampling Theorem, is a fundamental principle in the field of signal processing and telecommunications. It was first formulated by Harry Nyquist in 1928 and later expanded upon by Claude Shannon in his groundbreaking work on information theory.The theorem states that in order to perfectly reconstruct a continuous-time signal from its samples, the sampling rate must be at least twice the highest frequency present in the signal. This minimum rate is referred to as the Nyquist rate. If the sampling rate falls below this threshold, a phenomenon known as aliasing occurs, where the sampled signal becomes a distorted version of the original signal.The importance of the Nyquist Theorem lies in its application to digital signal processing, where analog signals must be converted into digital form for processing and storage. By adhering to the theorem's guidelines, engineers can ensure that the digital representation of an analog signal is accurate and free from aliasing.In practice, the Nyquist Theorem has implications for a wide range of technologies, from audio recording and broadcasting to medical imaging and seismology. It is a cornerstone concept that underpins the design of sampling systems and the development of anti-aliasing filters, which are used to prevent aliasing before the sampling process.The theorem also has a direct impact on the capacity of communication channels. In digital communication systems, understanding the relationship between the sampling rate and the frequency content of signals is crucial for maximizing the amount of information that can be transmitted without error.In summary, the Nyquist Theorem is a foundational principle that guides the process of sampling and reconstructing signals in digital systems. It ensures that high-quality digital representations of analog signals can be achieved, provided that the sampling rate is sufficiently high. This theorem has far-reaching applications and continues to be a key concept in the advancement of digital technology.。
Contributions to 2^nd TeV Particle Astrophysics Conference (TeV PA II) Madison Wisconsin -
a r X i v :a s t r o -p h /0611597v 2 22 N o v 2006Contributions to 2nd TeV Particle Astrophysics Con-ference (TeV PA II)Madison Wisconsin -28-31August 2006IceCube CollaborationA.Achterberg 31,M.Ackermann 33,J.Adams 11,J.Ahrens 21,K.Andeen 20,D.W.Atlee 29,J.N.Bahcall 25(deceased),X.Bai 23,B.Baret 9,S.W.Barwick 16,R.Bay 5,K.Beattie 7,T.Becka 21,J.K.Becker 13,K.-H.Becker 32,P.Berghaus 8,D.Berley 12,E.Bernardini 33∗,D.Bertrand 8,D.Z.Besson 17,E.Blaufuss 12,D.J.Boersma 20,C.Bohm 27,J.Bolmont 33,S.B¨o ser 33,O.Botner 30,A.Bouchta 30,J.Braun 20,C.Burgess 27,T.Burgess 27,T.Castermans 22,D.Chirkin 7,B.Christy 12,J.Clem 23,D.F.Cowen 29,28,M.V.D’Agostino 5,A.Davour 30,C.T.Day 7,C.De Clercq 9,L.Demir¨o rs 23,F.Descamps 14,P.Desiati 20,T.DeYoung 29,J.C.Diaz-Velez 20,J.Dreyer 13,J.P.Dumm 20,M.R.Duvoort 31,W.R.Edwards 7,R.Ehrlich 12,J.Eisch 26,R.W.Ellsworth 12,P.A.Evenson 23,O.Fadiran 3,A.R.Fazely 4,T.Feser 21,K.Filimonov 5,B.D.Fox 29,T.K.Gaisser 23,J.Gallagher 19,R.Ganugapati 20,H.Geenen 32,L.Gerhardt 16,A.Goldschmidt 7,J.A.Goodman 12,R.Gozzini 21,S.Grullon 20,A.Groß15,R.M.Gunasingha 4,M.Gurtner 32,A.Hallgren 30,F.Halzen 20,K.Han 11,K.Hanson 20,D.Hardtke 5,R.Hardtke 26,T.Harenberg 32,J.E.Hart 29,T.Hauschildt 23,D.Hays 7,J.Heise 31,K.Helbing 32,M.Hellwig 21,P.Herquet 22,G.C.Hill 20,J.Hodges 20,K.D.Hoffman 12,B.Hommez 14,K.Hoshina 20,D.Hubert 9,B.Hughey 20,P.O.Hulth 27,K.Hultqvist 27,S.Hundertmark 27,J.-P.H¨u lß32,A.Ishihara 20,J.Jacobsen 7,G.S.Japaridze 3,H.Johansson 27,A.Jones 7,J.M.Joseph 7,K.-H.Kampert 32,A.Karle 20,H.Kawai 10,J.L.Kelley 20,M.Kestel 29,N.Kitamura 20,S.R.Klein 7,S.Klepser 33,G.Kohnen 22,H.Kolanoski 6,L.K¨o pke 21,M.Krasberg 20,K.Kuehn 16,ndsman 20,H.Leich 33,D.Leier 13,M.Leuthold 1,I.Liubarsky 18,J.Lundberg 30,J.L¨u nemann 13,J.Madsen 26,K.Mase 10,H.S.Matis 7,T.McCauley 7,C.P.McParland 7,A.Meli 13,T.Messarius 13,P.M´e sz´a ros 29,28,H.Miyamoto 10,A.Mokhtarani 7,T.Montaruli 20,34,A.Morey 5,R.Morse 20,S.M.Movit 28,K.M¨u nich 13,R.Nahnhauer 33,J.W.Nam 16,P.Nießen 23,D.R.Nygren 7,H.¨Ogelman 20,A.Olivas 12,S.Patton 7,C.Pe˜n a-Garay 25,C.P´e rez de los Heros 30,A.Piegsa 21,D.Pieloth 33,A.C.Pohl 30,R.Porrata 5,J.Pretz 12,P.B.Price 5,G.T.Przybylski 7,K.Rawlins 2,S.Razzaque 29,28,E.Resconi 15,W.Rhode 13,M.Ribordy 22,A.Rizzo 9,S.Robbins 32,P.Roth 12,C.Rott 29,D.Rutledge 29,D.Ryckbosch 14,H.-G.Sander 21,S.Sarkar 24,S.Schlenstedt 33,T.Schmidt 12,D.Schneider 20,D.Seckel 23,S.H.Seo 29,S.Seunarine 11,A.Silvestri 16,A.J.Smith 12,M.Solarz 5,C.Song 20,J.E.Sopher 7,G.M.Spiczak 26,C.Spiering 33,M.Stamatikos 20,T.Stanev 23,P.Steffen 33,T.Stezelberger 7,R.G.Stokstad 7,M.C.Stoufer 7,S.Stoyanov 23,E.A.Strahler 20,T.Straszheim 12,K.-H.Sulanke 33,G.W.Sullivan 12,T.J.Sumner 18,I.Taboada 5,O.Tarasova 33,A.Tepe 32,L.Thollander 27,S.Tilav 23,M.Tluczykont 33,P.A.Toale 29,D.Turˇc an 12,N.van Eijndhoven 31,J.Vandenbroucke 5,A.Van Overloop 14,B.Voigt 33,W.Wagner 29,C.Walck 27,H.Waldmann 33,M.Walter 33,Y.-R.Wang 20,C.Wendt 20,C.H.Wiebusch 1,G.Wikstr¨o m 27,D.R.Williams 29,R.Wischnewski 33,H.Wissing 1,K.Woschnagg 5,X.W.Xu 4,G.Yodh 16,S.Yoshida 10,J.D.Zornoza 201III Physikalisches Institut,RWTH Aachen University,D-52056,Aachen,Germany 2Dept.of Physics and Astronomy,University of Alaska Anchorage,3211Providence Dr.,Anchorage,AK99508,USA3CTSPS,Clark-Atlanta University,Atlanta,GA30314,USA4Dept.of Physics,Southern University,Baton Rouge,LA70813,USA5Dept.of Physics,University of California,Berkeley,CA94720,USA6Institut f¨u r Physik,Humboldt Universit¨a t zu Berlin,D-12489Berlin,Germany7Lawrence Berkeley National Laboratory,Berkeley,CA94720,USA8Universit´e Libre de Bruxelles,Science Faculty CP230,B-1050Brussels,Belgium9Vrije Universiteit Brussel,Dienst ELEM,B-1050Brussels,Belgium10Dept.of Physics,Chiba University,Chiba263-8522Japan11Dept.of Physics and Astronomy,University of Canterbury,Private Bag4800,Christchurch,New Zealand12Dept.of Physics,University of Maryland,College Park,MD20742,USA13Dept.of Physics,Universit¨a t Dortmund,D-44221Dortmund,Germany14Dept.of Subatomic and Radiation Physics,University of Gent,B-9000Gent,Belgium15Max-Planck-Institut f¨u r Kernphysik,D-69177Heidelberg,Germany16Dept.of Physics and Astronomy,University of California,Irvine,CA92697,USA17Dept.of Physics and Astronomy,University of Kansas,Lawrence,KS66045,USA18Blackett Laboratory,Imperial College,London SW72BW,UK19Dept.of Astronomy,University of Wisconsin,Madison,WI53706,USA20Dept.of Physics,University of Wisconsin,Madison,WI53706,USA21Institute of Physics,University of Mainz,Staudinger Weg7,D-55099Mainz,Germany22University of Mons-Hainaut,7000Mons,Belgium23Bartol Research Institute,University of Delaware,Newark,DE19716,USA24Dept.of Physics,University of Oxford,1Keble Road,Oxford OX13NP,UK25Institute for Advanced Study,Princeton,NJ08540,USA26Dept.of Physics,University of Wisconsin,River Falls,WI54022,USA27Dept.of Physics,Stockholm University,SE-10691Stockholm,Sweden28Dept.of Astronomy and Astrophysics,Pennsylvania State University,University Park,PA16802,USA 29Dept.of Physics,Pennsylvania State University,University Park,PA16802,USA30Division of High Energy Physics,Uppsala University,S-75121Uppsala,Sweden31Dept.of Physics and Astronomy,Utrecht University/SRON,NL-3584CC Utrecht,The Netherlands 32Dept.of Physics,University of Wuppertal,D-42119Wuppertal,Germany33DESY,D-15735Zeuthen,Germany34on leave of absence University of Bari,70126,ItalyTable of Contents(i)Kael Hanson for the IceCube collaboration,Construction Status and Future of the IceCube NeutrinoObservatory(ii)Julia K.Becker for the IceCube collaboration,Implications of AMANDA Neutrino Flux Limits (iii)D.F.Cowen for the IceCube collaboration,Tau Neutrinos in IceCube(iv)Elisa Resconi for the IceCube collaboration,IceCube:Multiwavelength Search for Neutrinos from Transient Point Sources(v)Xinhua Bai and Thomas K.Gaisser for the IceCube collaboration,Air Showers in a Three-Dimensional Array:Recent Data from IceCube/IceTop(vi)Juan de Dios Zornoza for the IceCube collaboration,High-Energy Gammas from the Giant Flare of SGR1806-20of December2004in AMANDA(vii)Jon Dumm and Hagar Landsman for the IceCube collaboration,IceCube–First Results(viii)Jessica Hodges for the IceCube collaboration,Multi-Year Search for a Diffuse Flux of Muon Neutrinos with AMANDA-II(ix)Brennan Hughey for the IceCube collaboration,Searches for Neutrinos from Gamma-Ray Bursts with AMANDA-II and IceCubeConstruction Status and Future of the IceCube Neutrino ObservatoryKael D.Hanson1for the IceCube Collaboration1A3RI,University of Wisconsin-Madison,222W.Washington Ave,Madison,WI53703Abstract.The IceCube neutrino telescope nears the end of its second running season havingcollected a sample of over2×109triggered events.While the majority of these events are cosmicray muons,the detector is already sufficiently well understood to allow identification of neutrino-induced muon candidate events from the CR background.The production of optical moduleinstrumentation is now well-established,the modules themselves are functioning properly withlow failure rate,and it has been proven that the hot water drill can deliver the holes needed fordeployment of these instruments.The project plans to deploy12-14strings each year duringthe next several austral summers to bring the detector volume to1km3.1.IntroductionHigh-energy neutrino astrophysics is entering the era of kilometer-scale observatories.The IceCube neutrino telescope will be thefirst detector with an integrated exposure volume to reach1km3·yr.The detector includes a deep array of digital optical sensors deployed at depths between1500m and2450m in holes drilled in the glacial ice sheet at the geographic South Pole.These deep sensor modules detect the Cherenkov light radiated by passing charged relativistic particles in transit through the ice medium. The optical properties of this medium have been measured with in situ light sources[1]deployed with the predecessor detector array,AMANDA[2,3]:below1500m the ice becomes bubble free where long absorption and scattering lengths are found(ℓabs∼100m,ℓscatt∼25m).The IceCube deep array is optimized for the detection of muons produced by high energy(E≫1TeV) neutrinos from astrophysical point source emitters such as active galactic nuclei or transient sources such as gamma ray bursts[4].The muon is produced via charged-current interactions of the neutrino with ice nuclei(νµ+N→µ+X),typically exterior to the detector volume due to the long range of muons with energies in excess of1TeV.Ice is also an ideal calorimetric medium due to the long optical absorption lengths and so the visible energy of contained neutrino events can be reconstructed with±20%resolution in the exponent.In addition to these high-energy phenomena of cosmic origin,IceCube may observe signals from dark matter annihilations and will collect a high statistics sample(O(106))of atmospheric neutrinos relevant to particle physics topics such as Lorenz invariance tests in regions unreachable by other techniques.At the low energy end,IceCube presents an effective volume of approximately2.0×106tons to MeV neutrinos from supernovae.An array of the same sensors deployed in the ice holes are frozen into tanks at the top of each hole, providing an airshower detector component for IceCube.Called IceTop,this instrumentation may be used as a trigger veto to assist in rejection of cosmic ray event backgrounds in the deep detector.Furthermore, combining its data with data from the deep-ice array provides a unique opportunity to study cosmic ray composition in the region of the“knee,”extending earlier measurements performed using the combination of the SPASE and AMANDA detectors[5,6].Figure1.Surface view showing an overlay of the IceCube detector on the South Pole station map.The Amundsen-Scott station is slightly offthe lower righthand corner of this illustration. The existing AMANDA detector is represented by the concentric circles centered approximately on hole#48.Thick dots represent planned hole locations,those circled are either existing or planned in the2006-2007deployment season.2.Status of IceCube instrument deploymentThefirst IceCube string(#21)and thefirst four IceTop stations(#21,#29,#30,and#39)were deployed in January2005at the end of the deployment season and were operated during the austral winter of that year.The survivability of the digital optical modules during deployment and subsequent refreeze of the drill hole was established(all DOMs deployed during this season continue to function properly), useful performance data were gathered throughout the year of operation of the string[7,8],and neutrino candidate events were selected from this data run.During the following austral summer season,from December2005to January2006,eight more strings (#29,#30,#38,#39,#40,#49,#50,and#59)and twelve more IceTop stations(#38,#40,#47, #48,#49,#50,#57,#58,#59,#66,#67,and#74)were deployed bringing the count to9strings and 16surface stations and a total enclosed ice volume of0.1km3.Of the604sensors deployed to date,597 of them communicate and592are producing high quality data.A current view of the IceCube detector installation is shown in Figure1.The deployment plan calls for12-14strings and10surface stations to be deployed this year(2006-2007)to be followed by an average of14strings and IceTop stations in the following years until2011when the full complement of instrumentation will have been deployed, approximately70-75strings(60DOMs per string)and80surface stations(4DOMs per station).IceCube will be operated throughout the construction,achieving an integrated exposure of1km3·yr by2009and 4km3·yr by the second year of operation with the completed detector.We anticipate that the totaloperating lifetime of the experiment will be20years.3.Drilling and deploymentThe Enhanced Hot Water Drill(EHWD)system delivers2.5km×60cm holes to the deployment team for insertion of the optical sensor hardware.The system includes self-contained heating and electrical powerplants with a combined power of approximately5MW,pumping systems,a control facility,and drilling towers.Each year the drill camp is moved into place near the target holes.The towers then operate as mobilefield facilities served by the central drill camp and towed into position atop each drill hole(Figure2).During operation,the drill supplies200gallons per minute of190◦C water at1000psi. The average fuel consumed per hole is7200gallons.The entire operation of drilling a hole and deploying the optical module instrumentation takes approximately50hours.Figure2.IceCube drill camp with drill tower image inset in lower left corner.The tower sits atop a drill hole.Two icetop tanks forming a station are visible in trench to the right of the drill tower.4.The IceCube digital optical moduleThe IceCube digital optical module(DOM)(Figure3)is the central detector element used throughout the array,both in the deep ice and at the surface.It is a self-contained optical detector and data acquisition device.The analog optical device is a10”photomultiplier tube running at1.0×107gain into a∼50Ωfront-end load impedance.PMT high voltage bias is supplied internally by a DC-DC converter module that is powered from the+5V line on the DOM mainboard and can produce a programmable HV from 0to+2048V.A classical resistive divider bleeder distributes voltages to the PMT dynodes.The DOM also contains a PCB containing12405nm LEDs which may beflashed in the ice to provide a known optical source for studying ice properties or performing geometrical calibrations of the sensor array.All components are housed inside a0.5”thick glass pressure sphere rated to10000psi external pressure. The power and digital communication lines exit the DOM via the penetrator cable which attaches to the main communication cable bundles.DOM digital communication signals travel to the surface over copper quads contained within the45mm cable bundles.DOMs are assembled at three production and test facilities worldwide within the IceCube collaboration:University of Wisconsin,Stockholm University/Uppsala University,and DESY Zeuthen. Following assembly each DOM undergoes a2-3week test at various temperatures from+25◦C to−55◦C in order to evaluate its performance at low temperature and to characterize various optical and electronic operational parameters[9].All data thus far obtained with DOMs manufactured at all sites supports the claim that all sites are producing equivalent sensor hardware.To date2000of a total5000DOMs have been built.First pass yields are nearing90%and the shipping yields are in excess of95%.Figure3.IceCube digitial optical module,shown here without mounting harness for clarity. The PCB stack is visible with theflasher board module obscuring most of the DOM mainboard. The HV generator module is mounted on theflasher board(partially blocked in thisfigure by the penetrator assembly).The photomultiplier tube faces downward and here is almost completely hidden under the PCBs.5.Data acquisitionThe PMT pulses are converted into digital waveforms by one or more digitizer chips at speeds up to 3×108samples/s.Each DOM runs in self-triggered mode with the option to monitor digital trigger lines connected to its neighbor DOMs which it may use to influence the trigger decision.DOM-level triggers force a digitization and readout of the digitizers into local memory on the DOM(the DOM has a capacity of16MB)and each readout is time stamped with a counter value derived from the40MHz local DOM oscillator.Upon command from a surface controller,the DOM will transfer the contents of its memory buffers to the surface at a bit rate of1Mbit/s per copper pair.At the surface,DOMs are readout by specialized PCI cards plugged into industrial PCs running Linux.Software running inside these computers must translate the DOM timestamp to a global quantity since each DOM oscillator is free running.Therefore the time stamp generated in the DOM is only locally relevant.The time transformation is achieved by a process called RAPCal wherein the DOM and the surface digital communication hardware periodically(approximately once per second)exchange analog pulses and stamp the arrival and departure times.This information is used to establish the DOM clock to surface clock mapping.The clocks at the surface are driven from a single10MHz master clock signal synchronized to GPS.Measurements in the laboratory and in situ at South Pole demonstrate that DOM-to-DOM time jitter is O(3ns)less than the design specification of5ns.Once the digitized PMT pulses have been stamped with a global time,they are merged and sorted into a stream which is sent over ethernet to a cluster of trigger and event processor computers.The triggering and event packaging is accomplished entirely in application software.During the2006run, two triggers were implemented:a minimum bias trigger(MBT)generating an event trigger every n-th hit for system debugging and the main trigger for physics analysis,the simple majority trigger(SMT), requiring coincidence of8or more DOMs hit in the deep-ice array or6or more hits in the IceTop array within a time window of5µs.The triggers were formed in separate trigger processors for the in-ice and IceTop arrays;coincident triggers were then handled by a global trigger unit.Typical trigger rates froma run in mid-winter operation are listed in Table1.SMT5.28HzIceTop 6.43Hz0.25HzTable1.Trigger rates from a June23,2006data run.6.SummaryIceCube is soon to begin its3rd deployment season after concluding a successful deployment and running season.All indications from data quality verification studies point to the hardware functioning at or above its design specification.The detector will reach an integrated exposure volume of1km3·yr in as little as two years’time.Future detectors involving acoustic and radio detection techniques are being investigated as potential additions to the IceCube observatory to substantially extend the detector volume,particularly at higher energies.References[1]Ackermann M et.al.Optical properties of deep glacial ice at the South Pole2006J.Geophys.Res.111D13203[2]Andres E et.al.Observation of high-energy neutrinos using Cherenkov detectors embedded deep in Antarcticice2001Nature410441–3[3]Ahrens J et.al.Observation of high energy atmospheric neutrinos with the Antarctic muon and neutrinodetector array2002Phys.Rev.D6*******[4]Ahrens J et.al.Sensitivity of the IceCube detector to astrophysical sources of high energy muon neutrinos.2004Astropart.Phys.20507–32[5]Ahrens J et.al.Calibration and survey of AMANDA with the SPASE detectors2004Nucl.Instr.Meth.A522347–59[6]Bai X and Gaisser T Air showers in a three dimensional array:recent data from IceCube/IceTop2006thisproceedings[7]Achterberg A et.al.First year performance of the IceCube neutrino telescope2006Astropart.Phys.26155–73[8]Dumm J and Landsman H IceCube-first results2006this proceedings[9]Hanson K and Tarasova O Design and production of the IceCube digital optical module2006Proceedings ofthe4th International Conference on New Developments in Photodetection published in Nucl.Instr.Meth.A567214–7Implications of AMANDA neutrinoflux limitsJulia Becker for the IceCube Collaboration1Universit¨a t Dortmund,Institut f¨u r Physik,44221Dortmund,GermanyE-mail:julia.becker@Abstract.The Antarctic Muon And Neutrino Detector Array(AMANDA)is currently themost sensitive neutrino telescope at high energies.Data have been collected in a period of eightyears and analyzed with different analysis strategies.Limits to the neutrinoflux from pointsources,transient emissions,source catalogs and limits to different diffuseflux models havebeen obtained implying in some cases strong contraints to hadronic interaction models of suchsources.In this contribution,implications of the diffuse neutrino limit will be discussed withrespect to neutrino production mechanisms in astrophysical sources.1.Neutrinoflux predictionsThe existence of Ultra High Energy Cosmic Rays(UHECRs)as well as the detection of TeV photon emissions from galactic and extragalactic sources are a strong indication for neutrino(ν)emission from the same sources.Pions and kaons are believed to take a fraction of the proton energy producing TeV photons in coincidence with high energy neutrinos.Although the atmospheric background of neutrinos is quite high,it decreases rapidly with energy(∼E−3.7)while the extraterrestrial spectra of galactic and extra-galactic sources are typicallyflatter(typically∼E−2if shock acceleration is the main mechanism producing high energetic protons at the source).The latter should therefore become the dominant component of the total diffuse spectrum at a certain energy,which depends on the normalization of the neutrinoflux.Different predictions are shown in Fig.4.The left panel shows various calculations which use the diffuse X-ray background as measured by ROSAT to normalize the neutrino spectrum,see[1,2]. This is justified when assuming the production of neutrinos along with X-rays at the foot of jets of Active Galactic Nuclei(AGN)where protons are accelerated into the photon target of the disk.The right panel shows models based on the correlation between UHECRs,TeV photons and neutrinos,see[7,4].Such sources are optically thin to both TeV photons and protons.2.Detection techniques of AMANDAAMANDA detects muon-neutrinos(νµs)by observing secondary muons from charged current interactions of the neutrinos with the nucleons of the ice.The muons are traveling faster than light in ice and emit Cherenkov radiation which is detected by the photomultiplier tubes.Between the years2000and2004 data from effectively1001days have been taken and aνµsample of4282events from the Northern hemisphere has been collected2.In order to keep the analysis blinded to avoid experimenter’s bias, analyses cuts are optimized using off-source samples created by scrambling the right ascension of events or excluding the time window of transient emissions under investigation.For the case of diffuseflux analyses the analysis is optimized on a low energy sample,where the signal is expected to be negligible. 12Atmospheric muons make it impossible to use the Southern hemisphere forνµsearches.Cascade analyses can, however,be done for both hemispheres.These results will not be discussed here,but can be found in e.g.[5]E2Φlim=5.9·10−8GeV cm−2s−1(1) for5years of data taking.Here,E is the neutrino energy andΦlim is theflux upper limit.The search for single point sources was complemented by stacking classes of sources according to the direct correlation between the photon output and the potential neutrino signal.This was done for11 different AGN samples that were selected at different wavelength bands,see[6].The optimum sensitivity was typically achieved by the stacking of around10sources.The cumulative and mean source limit for every class is given in table2.In the diffuse analysis high-energy(HE)events from all directions are examined with respect to the spectral energy behavior of the sample.Aflattening of the total neutrino spectrum is expected when aflat,astrophysical component(Φ∼E−2)overcomes the steep atmospheric background(Φ∼E−3.7). The reconstructed energy spectrum for one year of data(year2000)is shown in Fig.4.It follows the atmospheric prediction(dashed lines).The most restrictive limit from the diffuse analysis for the years 2000to2003is given asE2Φlim=8.8·10−8GeV cm−2s−1sr−1(2) in the energy range of4.2<log(E/GeV)<6.4.The results were obtained by optimizing the analysis cuts on E−2spectra.Nonetheless the dependency of the response function of the detector to different spectra was considered and limits were set for different spectral shapes(e.g.E−3)or specific models as shown in Fig.4.Varying the spectral index in the simulation shows that the event distribution simulated for AMANDA peaks at very different energiesTable 2.Results of the stacking analysis for each AGN category[7]:thenumber ofincluded sourcesN src ,the number of expected events N bg νand the number of observed events N obs νare listed as well as the cumulative limit f lim as well as the limit per source f lim /N source ,both inunits of GeV cm −2s −1.N srcN obs νN bg νf lim f lim /N src 81725.72.710.34unidentified GeV sources114043.010.60.96keV blazars (HEAO-A)83133.49.71 1.2TeV blazars82429.5 5.940.74FR-I galaxies174057.2 2.910.17FR-II galaxies113541.6 6.700.61dE T eV <8.8·10−8GeV cm −2s −1sr −1.(3)Since TeV photons are absorbed on their way to Earth,current TeV Air Cherenkov telescopes can only detect sources up to z <0.3.TeV photons are believed to be directly correlated to HE neutrinos,since both are produced via the pions from the ∆-resonance resulting from p γinteractions.Thus,the detectedneutrinoflux from TeV observable sources isE2dNdET eV obs<1.7·10−9GeV cm−2s−1sr−1,(5)displayed as the dot-dashed line in the right panel of Fig.4.This underlines the necessity of a diffuse search with HE neutrino telescopes and the need for source-catalog independent searches.4.ConclusionsCurrently,AMANDA is the most sensitive neutrino telescope at high energies.Limits from5years for the point source analysis and four years for the diffuse analysis can already be used to constrain the physics of X-ray emission in AGN.Other acceleration mechanisms,predicting the emission of neutrinos in coincidence with TeV,GeV or MeV photons,are still very interesting to look for and represent interesting targets for observation.Optically thin sources are only observable in TeV photons up to z<0.3,which leaves neutrinos as a unique messenger from higher redshifts.IceCube is currently being built at the South Pole as AMANDA’s1km3-successor and the sensitivity will reach levels of E2Φsens∼(2−7)·10−9GeV cm−2s−1sr−1in only one year of full observation,see e.g.[11,12].This will allow to constrain further neutrino emissions from extragalactic sources,such as AGN and Gamma Ray Bursts(GRBs),or galactic sources such as micro-quasars and Supernova Remnants. AcknowledgmentsAcknowledgments from the IceCube collaboration can be found at .The author thanks the BMBF for the possibility to attend the conference(grant:05CI5PE1/0). References[1]Stecker F W and Salamon M H1996Space Science Reviews75341[2]Nellen L,Mannheim K and Biermann P L1993Phys.Rev.D475270[3]Mannheim K,Protheroe R J and Rachen J P2001Phys.Rev.D6323003[4]M¨u cke A et al.2003Astrop.Phys.18593[5]IceCube Collaboration,contributions to ICRC2005,Pune(India),astro-ph/0509330[6]Ackermann et al.2006,“On the selection of...”,accepted for publication in Astrop.Phys.[7]Achterberg et al.2006,to be submitted to Phys.Rev.D[8]Hodges J for the IceCube Collaboration2006,these proceedings[9]Becker J K,Rhode W,Biermann P L M¨u nich K2006astro-ph/0607427[10]Alvarez-Mu˜n iz J and M´e sz´a ros P2004Phys.Rev.D7012,123001[11]Hanson K for the IceCube collaboration2006,these proceedings[12]Halzen F2006,proc.of“The multimessenger approach to high-energyγ-ray sources”,Barcelona(Spain)Tau Neutrinos in IceCubeD.F.Cowen for the IceCube CollaborationPhysics Department,104Davey Laboratory,Pennsylvania State University,University Park,PA16802USAAbstract.Tau neutrino detection in IceCube would be strong evidence for the presence ofcosmologically-produced neutrinos.In addition to the well-known“double bang”signature,wedescribe herefive additional channels that we believe will not only extend the energy range overwhich IceCube can be sensitive to tau neutrinos,but also provide useful control over systematicuncertainties via self-consistency checks amongst all detection channels.1.IntroductionIn the search for ultrahigh energy neutrinos of cosmological origin,few pieces of evidence would be more convincing than a cleanly identified high energy tau neutrino.Tau neutrinos are not produced in standard cosmic-ray atmospheric interactions that create electron and muon neutrinos,and they are expected at immeasurably small levels in the prompt neutrinoflux created in charm particle decays in cosmic-ray interactions at high energies[1].Furthermore,at the energy and distance scales relevant for IceCube detection of atmospheric neutrinos,oscillations ofνe andνµintoντwill be very limited and will not result in large numbers ofντ’s at the detector.After ruling out all these possible high energyντsources, the only one left is a cosmological source that producesνe andνµthat oscillate over large travel distances to produce a measurable number ofντ’s at the detector.The standard UHE neutrino production mechanism is charged pion(and kaon)decay.Pion decay makes a neutrino beam with aflavor ratio ofνe:νµ:ντ=1:2:0.It is expected that neutrino oscillations will result in a1:1:1flavor ratio at the detector,and large deviations from this ratio would be an indication of new or unexpected physics,either in the production mechanism at the source,the propagation of the neutrinos over cosmological distances,or in the neutrino oscillation mechanism itself [2].Likewise,excessiveντ’s at atmospheric neutrino energies might also be an indicator of new physics, but IceCube will have limited ability to exclusively identifyντ’s at these lower energy scales,where a cascade from aντwill be very difficult to distinguish from a cascade from a charged-currentνe,a neutral-current any-flavor neutrino,or a low energy charged-currentνµinteraction.2.Tau Neutrino Signatures in IceCubeBy virtue of the tau lepton’s long decay length at ultrahigh energies,and its wide variety of decay modes, a tau produced in a charged-currentντinteraction has a rich set of possible signatures in the IceCube detector.The tau decay length is about50m per PeV,so a tau with Eτup to about20PeV can be fully contained in the detector volume.More generally,the tau production vertex,decay vertex or both may be observable in a single event.The tau can decay leptonically,τ→eνeντ(branching ratio=∼18%)or τ→µνµντ(∼18%),or hadronically,mainly to charged and neutral pions and kaons(∼64%).Note that since the average charged-currentντinteraction produces a tau with0.75Eν,we will assume Eν=Eτand refer to either as just“E”for the sake of simplicity.The following subsections and Figure1list six tau neutrino signatures to which IceCube may be sensitive.For each signature we also describe the chief expected backgrounds,energy range over which。
Neutrino Mass Spectrum and Future Beta Decay Experiments
c,d ‡
Scuola Internazionale superiore di Studi Avanzati via Beirut 4, I,34014 Trieste, Italy
b
Instituto de F´ ısica Gleb Wataghin, Universidade Estadual de Campinas, UNICAMP 13083-970 Campinas SP, Brazil The Abdus Salam International Centre for Theoretical Physics, I-34100 Trieste,Italy
hep-ph/0105105 February 1, 2008
Neutrino Mass Spectrum and Future Beta Decay Experiments
arXiv:hep-ph/0105105v3 26 May 2001
Y. Farzan
a
a ∗
, O. L. G. Peres
spectrum, one can put an upper bound on the neutrino mass assuming, however, that there is no conspiracy which leads to cancellation of different effects [4]. Therefore independent measurements of the neutrino mass are needed and their results will be used in the analysis of the cosmological data as an input deduced from particle physics. Several methods have been proposed to determine neutrino masses by using the supernova neutrino data. One method is based on searches for the energy ordering of events which has, however, rather low sensitivity [5]. The limits on the mass can be also obtained from observations of sharp time structures in the signals. It was suggested to study the time distribution of detected neutrino events emitted from supernova which entails to black hole formation [6]. By this method Super-Kamiokande can measure values of the νe mass down to 1.8 eV and SNO can put an upper bound 20 eV on the νµ and ντ masses [6] . (Clearly this bound on the νµ and ντ masses is much weaker than bounds implied by combined analysis of the solar and atmospheric neutrino data and direct measurements of the νe mass.) In this case one can check the still non-excluded possibility in which the solar neutrino problem is solved by the oscillations to sterile neutrino and the masses of νµ and ντ are in 20 eV range. (Such neutrinos should be unstable in cosmological time.) The absolute values of the neutrino masses can be determined in the assumption that the cosmic rays with energies above the GZK cutoff are produced in annihilation of the ultra-high energy neutrinos with the cosmological relic neutrinos [7, 8, 9]. The analysis of the observed energy spectrum of cosmic rays above 1020 eV gives the mass mν = (1.5 − 3.6) eV, if the power-like part of the ultra-high energy cosmic rays spectrum is produced in Galactic halo, and mν = (0.12 − 0.46) eV, if this part has the extragalactic origin [10]. Neutrinoless double beta decay (2β 0ν ) searches are sensitive to the Majorana mass of the electron neutrino. However, in the presence of mixing the situation can be rather complicated: The effective Majorana mass of νe relevant for the 2β 0ν -decay, mee , is a combination of mass eigenvalues and mixing parameters given by mee =
检验专业英语试题及答案
检验专业英语试题及答案一、选择题(每题2分,共20分)1. Which of the following is not a routine test in clinical laboratory?A. Blood countB. Urine analysisC. Liver function testD. DNA sequencing2. The term "hemoglobin" refers to:A. A type of proteinB. A type of enzymeC. A type of hormoneD. A type of lipid3. What is the primary function of the enzyme amylase?A. To break down proteinsB. To break down carbohydratesC. To break down fatsD. To break down nucleic acids4. The process of identifying the presence of a specific microorganism in a sample is known as:A. CulturingB. IsolationC. IdentificationD. Quantification5. Which of the following is a common method for measuring the concentration of glucose in blood?A. SpectrophotometryB. ChromatographyC. ElectrophoresisD. Enzymatic assay6. The term "ELISA" stands for:A. Enzyme-Linked Immunosorbent AssayB. Electrophoresis-Linked Immunosorbent AssayC. Enzyme-Linked Immunofluorescence AssayD. Electrophoresis-Linked Immunofluorescence Assay7. In medical diagnostics, what does "PCR" refer to?A. Polymerase Chain ReactionB. Protein Chain ReactionC. Particle Count ReactionD. Pathogen Characterization Reaction8. The process of measuring the amount of a specific substance in a sample is known as:A. TitrationB. CalibrationC. QuantificationD. Qualification9. Which of the following is a common type of clinical specimen?A. BloodB. SoilC. HairD. Water10. The term "antibodies" refers to:A. Proteins that recognize and bind to specific antigensB. Substances that neutralize toxinsC. Hormones that regulate immune responseD. Cells that produce immune responses二、填空题(每空1分,共10分)1. The process of separating molecules based on their size is known as __________.2. In clinical chemistry, the term "assay" refers to a__________ method.3. The unit of measurement for pH is __________.4. A common method for detecting the presence of antibodies in a sample is the __________ test.5. The process of identifying the type of bacteria in a sample is known as __________.6. The process of separating DNA fragments based on their size is known as __________.7. The term "ELISA" is used in __________ to detect the presence of specific antibodies or antigens.8. The process of identifying the genetic makeup of an organism is known as __________.9. The process of measuring the amount of a substance in a sample using a specific wavelength of light is called__________.10. The process of identifying the presence of specific microorganisms in a sample is known as __________.三、简答题(每题5分,共20分)1. Describe the principle of the Enzyme-Linked Immunosorbent Assay (ELISA).2. Explain the importance of maintaining aseptic technique ina clinical laboratory.3. What are the steps involved in performing a blood count?4. Discuss the role of antibodies in the immune response.四、论述题(每题15分,共30分)1. Compare and contrast the methods of Chromatography and Electrophoresis in terms of their applications in clinical diagnostics.2. Discuss the ethical considerations in the use of genetic testing for medical purposes.五、翻译题(每题5分,共10分)1. 将以下句子从中文翻译成英文:在临床实验室中,酶联免疫吸附测定法是一种常用的检测特定抗体或抗原的方法。
《最后的礼物》中的记忆书写和身份建构
‘齐齐哈尔大学学报“(哲学社会科学版)2024年2月Journal of Qiqihar University(Phi&Soc Sci) Feb.2024 收稿日期:2023-09-17 作者简介:王婉楹(2000-),女,在读硕士㊂主要从事文学伦理学批评研究㊂黄晖(1971-),男,教授,博士,博士生导师㊂主要从事非洲文学研究㊂ 基金项目:2019年国家社科基金重大项目:非洲英语文学史(19ZDA296);2017年国家社科基金后期资助项目:非洲文学史(17FWW001)‘最后的礼物“中的记忆书写和身份建构王婉楹,黄 晖(华中师范大学文学院,湖北武汉430079)摘 要:古尔纳的长篇小说‘最后的礼物“以非裔移民的回忆为故事线索,揭示两代移民之间代际记忆的断裂现象和身份建构危机㊂父辈的刻意遗忘与缄默不语造成子女的记忆危机和身份认同缺失,两代移民在沉默的记忆中都陷入了身份建构危机,既无法回溯过去重寻原生身份,又无法安于当下建构新的伦理身份,他们在复归和同化之间痛苦挣扎㊂古尔纳将言说与交流视为解除记忆危机和身份建构危机的有效手段,通过言说唤起个体记忆,个体记忆与集体记忆的交互作用促成了非裔移民对伦理身份的不断建构㊂关键词:古尔纳;‘最后的礼物“;记忆书写;伦理身份中图分类号:I106.4 文献标识码:A 文章编号:1008-2638(2024)02-0027-04Memory Writing and Identity Construction in The Last GiftWANG Wan -ying ,HUANG Hui(School of Chinese Language and Literature,Central China Normal University,Wuhan Hubei 430079,China) Abstract :Gurnah’s novel The Last Gift takes the memories of African immigrants as the story thread to reveal the inter-genera⁃tional memory breakdown and identity construction crisis between the two generations of immigrants.The parents’deliberate oblivionand silence lead to their children’s memory crisis andidentity absence.Both generations of immigrants fall into identity construction cri⁃sis in the silent memory,unable to trace back to the past to regain their original identity,and unable to settle for the present to build a new ethical identity,so they struggle painfully between return and assimilation.Gurnah regards explanation and communication as ef⁃fective ways to relieve the crisis of memory and identity construction,and evoke individual memory through explanation.The interaction between individual memory and collective memory contributes to the continuous construction of ethical identity by African immigrants. Key words :Gurnah;The Last Gift ;memory writing;ethical identity 2021年诺贝尔文学奖得主阿卜杜勒拉扎克㊃古尔纳(AbdulrazakGurnah)作为生活在英国的坦桑尼亚裔作家,他以书写难民的流散痛苦与身份认同危机为己任,诉说非裔难民的集体记忆,他的长篇作品‘最后的礼物“(The Last Gift ,2011)叙述了移民群体的尴尬处境和痛苦的过往记忆,揭露了移民英国的非裔群体无法逃避的身份认同困境,在小说中,古尔纳以回忆和现实交互穿插的方式将阿巴斯和玛丽亚姆的过去娓娓道来,阿巴斯对自己的记忆始终沉默不语,玛丽亚姆则是有选择性地言说记忆,但她隐藏的部分又是具有决定性作用的,因此同样可以看作是沉默的记忆,记忆的遗忘等同于身份认同的消除,阿巴斯和玛丽亚姆的无言昭示着他们的身份认同失败,同时在他们的沉默下,种族身份的记忆在代际传承中产生了断裂,第二代非裔移民汉娜和贾马尔对身份出处的无知和记忆缺失也诱发了他们的身份建构危机,主要表现为在同化和复归之间的焦虑和犹疑㊂最终在阿巴斯和玛丽亚姆的坦白下,他们的记忆作为 最后的礼物”唤起了两代人的回忆义务,促使四人重新思考自己的伦理身份,在过去与现在㊁第一代与第二代移民的记忆交互下,玛丽亚姆㊁汉娜与贾马尔选择弥补过去记忆的缺失,自觉肩负起传承非裔种族记忆的重任,重新建立起身份认同㊂一㊁沉默的记忆与身份建构失败在‘最后的礼物“中,古尔纳没有采用直线前进式的顺叙方法,而是通过回忆和现实交叉的跳跃性的叙事策略讲述了阿巴斯和玛利亚姆的一生,因此记忆既是小说的叙事主题,也是叙事动力㊂阿巴斯从家乡桑给巴尔逃跑后,在船上做水手开启小阿飞式的游荡生活,作为在全世界到处漂荡㊁居无定所的非裔,除了血液,仅剩记忆是他和桑给巴尔之间的密切联系,记忆是身份建构的关键,然而阿巴斯却在离家后主动且坚定地切断了这个纽带,他从未向任何人提及自己在桑给巴尔的过往,同时也极力忘记那段长期的记忆㊂玛利亚姆同作为流散在英国的非裔,几经辗转最终跟随阿巴斯私奔到诺维奇定居,她有选择性地对孩子们讲述自己幼年在多个寄养家庭短暂生活的记忆,而这些可知的记忆无不是美好㊁温暖的,她隐瞒了其中关键的部分记忆㊂无论是阿巴斯还是玛利亚姆,记忆不仅是他们作为流散非裔和过去或家乡的唯一联系,也是他们的人生经历和身份认同的确证, 回忆不仅位于历史和统治的中心,而且在建构个人和集体身份认同时都是秘密发挥作用的力量㊂”[1]对阿巴斯和玛利亚姆而言,沉默不语的记忆就意味着二人身份建构的失败㊂阿巴斯竭力逃离原生家庭和家乡,对过往记忆的闭口不言就是他对自我身份的主动舍弃,然而他在满世界流散的过程中也没有寻获到身份归属感,即使最后在英国定居并建立家庭,他也始终游离于社会边缘,于是他的身份建构无疑是失败的㊂阿巴斯对自己的原始身份是怀有自卑情绪的,由于吝啬鬼父亲的打压㊁贫穷的限制和社会的歧视,他始终处于敏感不安的情绪状态中,他将他人的目光解读成审视和鄙夷,婚后和谢法里一家生活时,他怀疑谢法里的两个哥哥明里暗里都在嘲笑他,他清楚地认识到自己和他们差距悬殊的种族身份和阶级身份,因此自卑敏感的他变得更加尖锐,所有负面情绪被无限放大,他无法忍受别人的蔑视㊂除此之外,阿巴斯怀疑未出世的孩子是谢法里和别人淫乱的结果,而不是自己的骨肉,他单方面认为谢法里一家同意这个婚姻是因为需要有个 倒霉蛋”来承担这个意外并维护自己的名声和尊严,这更加让阿巴斯感受到了羞辱㊂再加上让他窒息的家庭,吝啬的父亲不仅强制要求全家人日复一日地高强度劳动,而且将阿巴斯围困在闭塞的潇小乡村中,阻碍他前往更广阔的世界接受 无用”的教育, 在受到压迫的情况下,回忆可能变成一种反抗形式㊂”[2]在这样充满压迫的记忆中,他选择奋起反抗,抛弃了现有的一切,果断地逃离了所处的小世界,踏上了前往世界各地的船,在漂泊过程中他既在新加坡感受过自由,也在严苛不公的种族隔离制度中再次感受到对身份的抵触和抗拒,所以阿巴斯更加不愿回忆起过去, 遗忘却等于身份认同的消灭,”[1]他沉默的同时也是对建构已久的身份的瓦解㊂古尔纳并没有明示谢里夫的孩子究竟是不是阿巴斯的,如果阿巴斯真的是掉入了谢法里一家设置的陷阱,他的确是可悲的;如果孩子是阿巴斯的,那他的自卑和羞耻变得更加可笑且可悲㊂阿巴斯作为亲生父亲的这一身份的不明确性侧面印证了阿巴斯无法自我实现身份认同㊁建构㊂在阿巴斯沉默的记忆中,挥散不去的是他无法获得身份归属的漂泊不定感和异乡感,进而加速了其身份建构的失败㊂在迥然不同的异国他乡,阿巴斯无法逃避面临身份认同的困境,他处处展现出了格格不入的异乡人特征,特别体现在他的生活细节中:在寒冷的月份中居民们凭借长期的实践经验穿上合适㊁保暖的衣物,而阿巴斯没有穿够衣服, 尽管在这里生活了这么多年,却还是不知道”,[3]他不会囤积衣物,总是轻装出行,因为 这样等到他需要告别这里的时候,就可以将外套一把甩开”,[3]可见即使定居多年了,阿巴斯仍然没有将自己视作所处环境中的一份子,他在此处的记忆是暂时且不稳定的,他的身份如浮萍般漂泊无根,没有顺利建构身份认同㊂阿巴斯并非彻底放弃身份建构,尽管他对记忆只字不提,但他从未真正遗忘过去,所谓的沉默只是他表面作出的努力,他内心仍然渴望获得身份认同,完成牢固的身份建构㊂他将记忆的长久性寄托在衣物上,他将衣服穿到破为止,因为熟悉的衣服及其带来的回忆可以让他 一眼认出自己来”㊂[3]阿巴斯搬家时仅带几件旧物走,可见他空无一物的流浪者形象已经在他潜意识中根深蒂固,然而搬入新空间时他主动做出一系列的调整:修缮㊁种植和建造,将尚未熟悉的空间改造成自认舒适的环境,这是阿巴斯作为流散者对长期坚持的生活环境和生活方式的固守,既说明阿巴斯的记忆不仅没有真正被遗忘㊂而且也服务于他想要建立起与过往记忆空间相平行的空间的尝试,通过这种尝试,他在其中试图稳固身份认同和身份建构㊂在他中风晕倒之际,他希望 等到有双强有力的臂膀过来将他抱起,带他回家”,[3]同时他认为自己是个 罪孽深重的游子,虚度了全然徒劳的一生后,在一片陌生的土地上害了病㊂”[3]他对在异乡死去这件事既感到恐惧又悲伤,此时他意识到自己身份建构的失败,归乡的隐藏意识逐渐清晰,于是他开始有意愿想要回忆并诉说自己的记忆,意识到只有言说才能疗救他的记忆创伤,完成身份建构,最终他将过往记忆以录音的形式记录下来作为 最后的礼物”送给玛利亚姆㊁汉娜和贾马尔㊂不同于阿巴斯对记忆的对抗和反叛,玛丽亚姆对记忆则是有选择性地回忆和遗忘,她试图通过对部分记忆保持沉默来腾出更多接纳空间以便按照自我意愿来构建身份和展开新生活,但她隐匿的部分记忆是无法遗忘的,无法接受并与这类关键性的记忆融洽相处就意味着她的身份建构失败的必然㊂从出生便被父母遗弃而居无定所的玛利亚姆是个没有确切身份的孤儿,她没有可以产生记忆的固定空间场所㊁固定客体对象以及固定生活方式,因此她从来没有建构起过真正牢固的自我身份㊂尽管玛丽亚姆辗转于多个寄养家庭拥有着并不单调的记忆,但玛利亚姆总是有选择性地回忆并讲述,她只回忆美好的一面,刻意地忽略掉那些艰难㊁受辱㊁被压迫的细节,自发地塑造出一个充满困厄但温馨有爱的人生经历㊂她试图在过去的回忆中寻求身份建构,她把并不确定存在地米色襁褓作为关键记忆点,始终牢记这个物品来寻求身份归属感, 有时候她一摸到那襁褓,就会想到,我的母亲”,[3]玛丽亚姆会通过自己选择记忆从而进行身份建构㊂除此之外,玛丽亚姆只对孩子们简单提及过与费鲁兹夫妇成为一家人的温暖记忆,将自己归属为费鲁兹家庭中的一员,主动做家务实现个人价值,认为这是自己理应付出的,但她却不曾提起后来受到的屈辱和轻视以及迪尼斯的猥亵,她长久以来为身份构建做的努力在迪尼斯到来之后顷刻间付之一炬, 一想到费鲁兹和维贾伊,她就浑身一哆嗦,回回都是,哪怕过了这么多年;她舒展了一下肩膀和脖颈,然后轻轻地82齐齐哈尔大学学报(哲学社会科学版) 把这段记忆推开,”[3]但这些无法忘却的关键记忆又时刻提醒着她:她依旧是无价值㊁无归属的人,尽管她随阿巴斯逃离了埃克塞特,前往新的地方定居,重新选择并组织起自己的记忆以实现身份建构,但她依然无法认同自己是埃克塞特的费鲁兹一家的人,也无法立刻使自己融入当下所处环境中,所以在这种认知的断裂下,玛利亚姆也没有实现真正的身份认同㊂扬㊃阿斯曼将记忆分成两种形式:交往记忆和文化记忆,阿巴斯和玛利亚姆一直以来想要掩盖的记忆正是较为私人的㊁口头的㊁日常的交往记忆,这种交往记忆是以个体生平为框架并且在集体交往中形成的, 随着它的承载者而产生并消失,”[2]其典型范例是代际记忆,然而阿巴斯和玛利亚姆的沉默导致了交往记忆的退场㊁代际记忆的割裂,他们的子女对家庭历史一无所知,同时阿巴斯也不主张他们参加穆斯林相关的仪式活动,他们的生活并无穆斯林文化的渗透,阿巴斯不仅切断了代际记忆的延续,而且也使其一家人从巩固身份认同的文化记忆中抽离出来,如此一来,有身份建构困扰的不止是已然失败的阿巴斯和玛利亚姆这类第一代移民,还有第二代移民㊂二㊁缺失的记忆与身份建构焦虑家庭㊁社会带给阿巴斯夫妇的沉重创伤促使二者选择遗忘过往记忆,交往记忆 可被视为一个社会的短期记忆,注定存在于活着的记忆承载者身上,存在于交往对象的体验当中,涵盖了三至四代人㊂”[4]作为文化承载者的阿巴斯夫妇的沉默,导致了下一代非裔移民对种族身份意识的缺失,造成了汉娜和贾马尔的身份建构焦虑,由于对出身的无知,他们无法通过记忆叙事来界定并理解自己的身份,也无法深入体验自己与世界的关系㊂尽管他们是在英国出生并成长,但是他们仍然是难以得到英国社会认同的边缘人,对此汉娜和贾马尔游离在非裔身份复归和英国化之间,两种伦理选择的博弈使他们愈发焦虑不安㊂汉娜缺失的记忆致使她无法对非裔移民产生身份认同,她努力融入英国白人社会,试图在其中完成身份建构,但她在英国人面前仍然是 他者”的存在,处于悬殊的低位㊂汉娜对家族记忆的态度从幼时到大学时期经历了转变,幼小的汉娜面对父母缄默不语的记忆有着强烈的好奇,她认为一定要知道家庭的历史和出身的真相,而不是像阿巴斯和玛利亚姆一样沉默,这种怪异的沉默是完全没有必要的,这让汉娜感觉 过的是一种偷偷摸摸㊁耻于见光的人生㊂”[3]此时的汉娜表现得正如年轻的阿巴斯一样无所畏惧,永远坚信自己,她想要坦诚的记忆,想要 能够大大方方地说,这就是我㊂”[3]然而汉娜并没有成功地揭开记忆的秘密,本科生汉娜将自己的家庭称为 缺陷家庭”,她不再执着于记忆,而是放弃了过去的记忆,转向构建能够融入美国白人社会的身份,她在外将自己的名字改作更加 英国化”的安娜,甚至潜移默化地站在白人视角凝视非裔,对待需要帮助的黑人妇女,她表现出来的是歧视和厌烦,她已经完全将自己也是非裔移民这一记忆和身份撇除了,明显变成了阿巴斯并不期望的 英国姑娘”㊂汉娜这种身份建构的尝试并没有为她赢得白人的接纳,她在白人男友及其家庭面前,依然是被排挤的 他者”㊂男友父亲拉尔夫在第一次见面的餐桌上对非洲问题侃侃而谈,站在殖民者的角度看待非洲,彰显出不可比拟的优越感,下意识地将汉娜当作非洲群体的代表,尽管汉娜生在英国并渴望 英国化”,但还是会被迪格比追问成为英国人之前是哪里人,汉娜对男友一家的偏见没有任何反驳和争论,因为她苦心经营的身份建构并没有被她想要融入的集体所承认㊂男友家庭的歧视和排挤反而加强了汉娜自身的非裔身份认同,她的身份建构呈现出复归和同化共存的矛盾状态㊂在搬家时,汉娜既会保留原先的旧物,也会果断丢弃旧物以容纳新物品,搬家途中总会损坏的旧物象征着不断被威胁的原生身份和磕磕碰碰的身份建构过程,在搬家机构帮忙搬运时,汉娜面对他们的介入感到十分沮丧,无论是搬家的物品还是个人身份建构,汉娜本应拥有个人选择权,但缺失的记忆和英国文化的强势话语都让汉娜陷入无助和焦虑中㊂汉娜表面上 英国化”的同时,她潜意识里却是背道而驰的复归意向,她频繁地做着同一个充满隐喻意义的梦,梦中 她住在房子的半边屋宇里,剩下的半边早已年久失修:顶梁塌陷,木窗咯吱作响,近乎朽烂㊂”[3]这一半完好一半残败的房子正如同汉娜一样,非裔移民和英国姑娘两种矛盾身份的结合体,她处于完好的一半屋宇中,另一半残败的屋宇 带着恶意,带着警觉,带着指责㊂”[3]梦里她心中充斥着负罪感,这种内疚促使她想要修缮残败的那一半房子,这意味着她的内心对自己 英国化”的谴责,她想要采取某种手段实现身份复归,想要修补那段缺失的记忆,然而她既不认识建筑工也不认识木工,她在父辈的沉默中无从寻找记忆的线索㊂贾马尔对记忆的态度呈现出和玛利亚姆的相似性,他同样选择回忆美好的部分,耐心地聆听着父辈的记忆诉说,以此形成独特的个人经验,反思移民问题,正是如此,他的身份建构焦虑也远远轻于汉娜㊂贾马尔并不偏执地想要父辈坦承一切,而是将慢慢浮现的记忆碎片拼凑起来,尽管他将自己的学术研究聚焦于流散者群体上,但他缺失的记忆令他无法贴近流散者的本质㊂相较于汉娜,贾马尔的身份建构焦虑没有让他低落,而是促使他更加坚定地重构非裔身份,从容地构建起跨族群的双重身份认同㊂比起迫切再塑熟悉环境的阿巴斯,或者是需要更多自主权的汉娜,贾马尔在搬家过程中的反应彰显了他的身份适应性,贾马尔搬家时没有任何旧物,格外轻便地搬进房东已准备好的公寓,在新公寓中,他和来自不同群体的人都可以和谐相处,说明他有足够包容的接纳空间可以选择同化㊂同时,贾马尔在学习过程中感受到族裔群体和外部世界的冲突,邻居哈伦的叙述和父母的坦白都让他更加贴近流散者群体,也使贾马尔确认了自身的非裔身份和立场,并由此出发关注流散者群体身份认同问题㊂对于第二代移民汉娜和贾马尔而言,父母言说的记忆和家庭历史是帮助流散者建构身份的基本途径,想要顺利完成新阶段的身份建构,就要先对原生身份进行解构,但汉娜和贾马尔对原生身份的知情权㊁其所属群体身份的一致性和关联性都被记忆的静默打断,所以二人的两难处境使其在同化和复归之间始终游移不定,滞于身份建构的焦虑中㊂三㊁记忆交互与伦理身份建构阿巴斯和玛利亚姆强迫自己遗忘记忆导致二人不肯承92 第2期‘最后的礼物“中的记忆书写和身份建构认来处又不知该何去何从,记忆的空缺致使汉娜和贾马尔在被英国社会同化或是重寻非裔身份之间犹疑不定,四人的身份建构危机皆是源自沉默的记忆,因此想要巩固群体身份认同, 这类知识要实现其在构建统一体㊁提供行动指南方面(即规范性的和定型性的)的推动力,就必须首先具备这三个作用:存储㊁调取㊁传达,”[2]也就是说身份建构的先决条件是记忆㊁回忆㊁言说, 回忆着的群体通过忆起过去,巩固了其认同㊂”[2]中风使一直以来沉默的阿巴斯更加深切地体悟到了身份建构失败的痛楚,他开始想要言说自己的记忆却无法发声,他于病榻上回想自己的一生,最终将自己的记忆通过录音带讲述给家人,录音带作为个体记忆的承载物可以不断巩固和重复记忆,达到了以个人生平为主线的交往记忆构建身份认同和群体记忆的作用㊂尽管阿巴斯直至死亡都没有解除自己的身份建构危机,但他的自述鼓舞了妻子玛利亚姆直面自己的创伤记忆,进而更为积极主动地实现个人价值,重新确认自身的伦理身份㊂玛利亚姆向汉娜和贾马尔坦言自己被轻视㊁被侵犯的记忆,她的诉说代表她和过去的创伤已经和解,接受了自身的身份建构是由全部记忆成就的,而非仅凭美好的记忆㊂在过去种种苦难的感召下,她内在强化了作为非裔移民的伦理意识,所以她选择去难民中心做志愿服务,想要尽可能地帮助更多和她有着相似经历的人,她的这一决定也得到了家人的支持,她借此也寻找到了个人价值㊂在和阿巴斯的记忆交互中,玛利亚姆意识到自己的创伤记忆并不是羞耻的㊁罕见的,她所遭受的一切是非裔群体所普遍经历过的,而不是她个人的不幸,通过这种苦难记忆的相似,将玛利亚姆和非裔群体凝聚起来,加强她的归属感,因此她重新确认了自身的伦理身份,在伦理意识驱使下,她不再囿于费鲁兹㊁维贾伊和迪尼斯带给她的痛苦记忆中,她联络到费鲁兹并回到埃克塞特与他们相认,她不再迷失寻不到出路,在家人的支持和记忆的言说下,她确证了自身身份建构㊂记忆的言说不仅使阿巴斯和玛利亚姆得到了精神解脱,而且弥补了汉娜和贾马尔对出身认知的空白,二人的身份建构也经历了由摇摆到坚定的历程㊂作为非裔移民的后裔,大学时期的汉娜对这一伦理身份十分抵触,所以她活跃在英国本土年轻人的社交圈中,想要变成英国姑娘㊂但随着她和男友及其家庭交往的逐渐深入,她越来越感觉到自己受到的蔑视和偏见,同时她也越来越失落,通过她反复做的那场梦可以发现她的失落感来源于自身对家族㊁种族记忆的空白,她从原生种族群体剥离出来,漂泊感和不安感席卷而来,汉娜开始意识到寻获记忆㊁重构伦理身份的重要性㊂阿巴斯的死亡和坦白无疑使汉娜和贾马尔更加坚定内心,阿巴斯和玛利亚姆将个人记忆通过叙述言说进而形成家族范围内的集体记忆, 群体记忆的意思是个体通过把自己置于群体的位置来进行回忆,或者说,群体的记忆是通过个体记忆来实现的,并且在个体记忆之中体现自身㊂”[5]在一代移民和二代移民的记忆交互中,四人逐渐形成一个记忆共同体,记忆的传播和分享伴随着情感的交流互动,汉娜㊁贾马尔在记忆的陈述中产生个体情感体验和伦理意识,在这种交流中二人在被英国社会同化和寻找非裔移民记忆之间不再犹豫不决,而是做出自己认为正确的选择,伦理选择是 人的本质的选择”[6],二人选择成为情感自洽的非裔正是对自己本质身份的回归㊂虽然汉娜和贾马尔选择并完成了伦理身份的复归,但他们并没有止步于此,而是开始思考成为非裔集体记忆的传承者,这同样是二人回归非裔群体中责任感的体现㊂在帮助玛利亚姆和养父母重逢并实现身份建构后,三人计划代替已逝的阿巴斯回到故乡桑给巴尔以完成家族记忆的缝合和补充,这种记忆交互对身份建构具有重大意义,个体记忆通过言说进行交换与扩张,经由伦理意识共鸣转化成代际记忆,从而促进下一代对非裔移民这一伦理身份的认同与重建㊂总之,对桑给巴尔人而言,被英国殖民统治的记忆和国内屠杀记忆是永远无法磨灭的记忆,尽管许多人以难民身份逃脱出来,但这种创伤记忆和流散经历已成为他们的身份标记㊂古尔纳作为流散作家,他积极确认并承担文学责任,书写个人的同时更加关注种族身份建构的问题,表达出具有强烈种族责任感的非裔作家对非裔移民流散各处后身份重建的忧惧和思考㊂在‘最后的礼物“中,古尔纳尤为重视记忆和身份建构的密切联系,以阿巴斯和玛利亚姆的回忆展开叙述,展现了流散移民的记忆危机和身份建构危机,进而揭示了非裔移民的记忆和现实境遇的矛盾和冲突,这不仅表现在代际记忆的割裂上,也表现在第二代非裔移民在同化和复归之间挣扎的伦理选择上㊂面对非裔移民的记忆危机和身份建构危机时,或许只有言说才是解决问题的有效手段,通过言说唤起个体记忆,将个体记忆转换成集体记忆,在记忆的分享与互动中,凝聚形成情感共同体,促进伦理意识的觉醒,最终完成伦理身份的认同与建构㊂ 参考文献:[1](德)阿莱达㊃阿斯曼.回忆空间:文化记忆的形式和变迁[M].潘璐,译.北京:北京大学出版社,2016:63,68.[2](德)扬㊃阿斯曼.文化记忆:早期高级文化中的文字㊁回忆和政治身份[M].金寿福,黄晓晨,译.北京:北京大学出版社,2015:69,44,51,47.[3](英)阿卜杜勒拉扎克㊃古尔纳.最后的礼物[M].宋佥,译.上海:上海译文出版社,2022.[4](德)阿斯特莉特㊃埃尔.文化记忆研究指南[M].李恭忠,李霞,译.南京:南京大学出版社,2018:353.[5](法)莫里斯㊃哈布瓦赫.论集体记忆[M].毕然,郭金华,译.上海:上海人民出版社,2002:71.[6]聂珍钊.文学伦理学批评导论[M].北京:北京大学出版社,2014:267.[7]朱振武,游铭悦.身份认同与共同体意识 最新诺奖作家古尔纳‘最后的礼物“的创作旨归[J].山东外语教学,2 (2022):71-82.(责任编辑 吴明东) 03齐齐哈尔大学学报(哲学社会科学版) 。
tpo61三篇托福阅读TOEFL原文译文题目答案背景知识
tpo61三篇托福阅读TOEFL原文译文题目答案背景知识阅读-1 (2)原文 (2)译文 (5)题目 (7)答案 (13)背景知识 (15)阅读-2 (18)原文 (19)译文 (22)题目 (24)答案 (32)背景知识 (34)阅读-3 (39)原文 (39)译文 (42)题目 (45)答案 (53)背景知识 (54)阅读-1原文Physical Properties of Minerals①A mineral is a naturally occurring solid formed by inorganic processes. Since the internal structure and chemical composition of a mineral are difficult to determine without the aid of sophisticated tests and apparatus , the more easily recognized physical properties are used in identification.②Most people think of a crystal as a rare commodity, when in fact most inorganic solid objects are composed of crystals. The reason for this misconception is that most crystals do not exhibit their crystal form: the external form of a mineral that reflects the orderly internal arrangement of its atoms. Whenever a mineral forms without space restrictions, individual crystals with well-formed crystal faces will develop. Some crystals, such as those of the mineral quartz, have a very distinctive crystal form that can be helpful in identification. However, most of the time, crystal growth is interrupted because of competition for space, resulting in an intergrown mass of crystals, none of which exhibits crystal form.③Although color is an obvious feature of a mineral, it is often anunreliable diagnostic property. Slight impurities in the common mineral quartz, for example, give it a variety of colors, including pink, purple (amethyst), white, and even black. When a mineral, such as quartz, exhibits a variety of colors, it is said to possess exotic coloration. Exotic coloration is usually caused by the inclusion of impurities, such as foreign ions, in the crystalline structure. Other minerals —for example, sulfur, which is yellow, and malachite, which is bright green —are said to have inherent coloration because their color is a consequence of their chemical makeup and does not vary significantly.④Streak is the color of a mineral in its powdered form and is obtained by rubbing a mineral across a plate of unglazed porcelain. Whereas the color of a mineral often varies from sample to sample, the streak usually does not and is therefore the more reliable property.⑤Luster is the appearance or quality of light reflected from the surface of a mineral. Minerals that have the appearance of metals, regardless of color, are said to have a metallic luster. Minerals with a nonmetallic luster are described by various adjectives, including vitreous (glassy) pearly, silky, resinous, and earthy (dull).⑥One of the most useful diagnostic properties of a mineral is hardness, the resistance of a mineral to abrasion or scratching. This property is determined by rubbing a mineral of unknown hardness against one ofknown hardness, or vice versa. A numerical value can be obtained by using Mohs' scale of hardness, which consists of ten minerals arranged in order from talc, the softest, at number one, to diamond, the hardest, at number ten. Any mineral of unknown hardness can be compared with these or with other objects of known hardness. For example, a fingernail has a hardness of 2.5, a copper penny 5, and a piece of glass 5.5. The mineral gypsum, which has a hardness of two, can be easily scratched with your fingernail. On the other hand, the mineral calcite which has a hardness of three, will scratch your fingernail but will not scratch glass. Quartz, the hardest of the common minerals, will scratch a glass plate.⑦The tendency of a mineral to break along planes of weak bonding is called cleavage. Minerals that possess cleavage are identified by the smooth, flat surfaces produced when the mineral is broken. The simplest type of cleavage is exhibited by the micas. Because the micas have excellent cleavage in one direction, they break to form thin, flat sheets. Some minerals have several cleavage planes, which produce smooth surfaces when broken, while others exhibit poor cleavage, and still others exhibit no cleavage at all. When minerals break evenly in more than one direction, cleavage is described by the number of planes exhibited and the angles at which they meet. Cleavage should not be confused with crystal form. When a mineral exhibits cleavage, itwill break into pieces that have the same configuration as the original sample does. By contrast, quartz crystals do not have cleavage, and if broken, would shatter into shapes that do not resemble each other or the original crystals. Minerals that do not exhibit cleavage are said to fracture when broken. Some break into pieces with smooth curved surfaces resembling broken glass. Others break into splinters or fibers, but most fracture irregularly.译文矿物的物理性质①矿物质是由无机过程形成的天然固体。
GMAT阅读练习(10)
Passage 1 Virtually everything astronomers known about objects outside the solar system is based on the detection of photons-quanta of electromagnetic radiation. Yet there is another form of radiation that permeates the universe: (5) neutrinos. With (as its name implies) no electric charge, and negligible mass, the neutrino interacts with other particles so rarely that a neutrino can cross the entire universe, even traversing substantial aggregations of matter, without being absorbed or even deflected. Neu- (10) trinos can thus escape from regions of space where light and other kinds of electromagnetic radiation are blocked by matter. Furthermore, neutrinos carry with them information about the site and circumstances of their production: therefore, the detection of cosmic neutrinos (15) could provide new information about a wide variety of cosmic phenomena and about the history of the uni- verse. But how can scientists detect a particle that interacts so infrequently with other matter? Twenty-five years (20) passed between Pauli's hypothesis that the neutrino existed and its actual detection: since then virtually all research with neutrinos has been with neutrinos created artificially in large particle accelerators and studied under neutrino microscopes. But a neutrino telescope, (25) capable of detecting cosmic neutrinos, is difficult to co- nstruct. No apparatus can detect neutrinos unless it is extremely massive, because great mass is synonymous with huge numbers of nucleons (neutrons and protons), and the more massive the detector, the greater the pro- (30) bability of one of its nucleon's reacting with a neutrino. In addition, the apparatus must be sufficiently shielded from the interfering effects of other particles. Fortunately, a group of astrophysicists has proposed a means of detecting cosmic neutrinos by harnessing the (35) mass of the ocean. Named DUMAND, for Deep Under- water Muon and Neutrino Detector, the project calls for placing an array of light sensors at a depth of five kilo- meters under the ocean surface. The detecting medium is the seawater itself: when a neutrino interacts with a (40) particle in an atom of seawater, the result is a cascade of electrically charged particles and a flash of light that can be detected by the sensors. The five kilometers of sea- water above the sensors will shield them from the interf- ering effects of other high-energy particles raining down (45) through the atmosphere. The strongest motivation for the DUMAND project is that it will exploit an important source of information about the universe. The extension of astronomy from visible light to radio waves to x-rays and gamma rays (50) never failed to lead to the discovery of unusual objects such as radio galaxies, quasars, and pulsars. Each of these discoveries came as a surprise. Neutrino astronomy will doubtless bring its own share of surprises. 1. Which of the following titles best summarizes the passage as a whole? (A) At the Threshold of Neutrino Astronomy (B) Neutrinos and the History of the Universe (C) The Creation and Study of Neutrinos (D) The DUMAND System and How It Works (E) The Properties of the Neutrino 2. With which of the following statements regarding neutrino astronomy would the author be most likely to agree? (A) Neutrino astronomy will supersede all present forms of astronomy. (B) Neutrino astronomy will be abandoned if the DUMAND project fails. (C) Neutrino astronomy can be expected to lead to major breakthroughs in astronomy. (D) Neutrino astronomy will disclose phenomena that will be more surprising than past discoveries. (E) Neutrino astronomy will always be characterized by a large time lag between hypothesis and experimental confirmation. 3. In the last paragraph, the author describes the development of astronomy in order to (A) suggest that the potential findings of neutrino astronomy can be seen as part of a series of astronomical successes (B) illustrate the role of surprise in scientific discovery (C) demonstrate the effectiveness of the DUMAND apparatus in detecting neutrinos (D) name some cosmic phenomena that neutrino astronomy will illuminate (E) contrast the motivation of earlier astronomers with that of the astrophysicists working on the DUMAND project 4. According to the passage, one advantage that neutrinos have for studies in astronomy is that they (A) have been detected for the last twenty-five years (B) possess a variable electric charge (C) are usually extremely massive (D) carry information about their history with them (E) are very similar to other electromagnetic particles 5. According to the passage, the primary use of the apparatus mentioned in lines 24-32 would be to (A) increase the mass of a neutrino (B) interpret the information neutrinos carry with them (C) study the internal structure of a neutrino (D) see neutrinos in distant regions of space (E) detect the presence of cosmic neutrinos 6. The passage states that interactions between neutrinos and other matter are (A) rare (B) artificial (C) undetectable (D) unpredictable (E) hazardous 7. The passage mentions which of the following as a reason that neutrinos are hard to detect? (A) Their pervasiveness in the universe (B) Their ability to escape from different regions of space (C) Their inability to penetrate dense matter (D) The similarity of their structure to that of nucleons (E) The infrequency of their interaction with other matter 8. According to the passage, the interaction of a neutrino with other matter can produce (A) particles that are neutral and massive (B) a form of radiation that permeates the universe (C) inaccurate information about the site and circumstances of the neutrino's production (D) charged particles and light (E) a situation in which light and other forms of electromagnetic radiation are blocked 9. According to the passage, one of the methods used to establish the properties of neutrinos was (A) detection of photons (B) observation of the interaction of neutrinos with gamma rays (C) observation of neutrinos that were artificially created (D) measurement of neutrinos that interacted with particles of seawater (E) experiments with electromagnetic radiation Passage 2 Since the late 1970's, in the face of a severe loss of market share in dozens of industries, manufacturers in the United States have been trying to improve produc- tivity-and therefore enhance their international (5) competitiveness-through cost-cutting programs. (Cost- cutting here is defined as raising labor output while holding the amount of labor constant.) However, from 1978 through 1982, productivity-the value of goods manufactured divided by the amount of labor input- (10) did not improve; and while the results were better in the business upturn of the three years following, they ran 25 percent lower than productivity improvements during earlier, post-1945 upturns. At the same time, it became clear that the harder manufacturers worked to imple- (15) ment cost-cutting, the more they lost their competitive edge. With this paradox in mind, I recently visited 25 companies; it became clear to me that the cost-cutting approach to increasing productivity is fundamentally (20) flawed. Manufacturing regularly observes a “40, 40, 20” rule. Roughly 40 percent of any manufacturing-based competitive advantage derives from long-term changes in manufacturing structure (decisions about the number, size, location, and capacity of facilities) and in approaches (25) to materials. Another 40 percent comes from major changes in equipment and process technology. The final 20 percent rests on implementing conventional cost- cutting. This rule does not imply that cost-cutting should not be tried. The well-known tools of this approach- (30) including simplifying jobs and retraining employees to work smarter, not harder-do produce results. But the tools quickly reach the limits of what they can contribute. Another problem is that the cost-cutting approach (35) hinders innovation and discourages creative people. As Abernathy's study of automobile manufacturers has shown, an industry can easily become prisoner of its own investments in cost-cutting techniques, reducing its ability to develop new products. And managers under (40) pressure to maximize cost-cutting will resist innovation because they know that more fundamental changes in processes or systems will wreak havoc with the results o n / p > p b d s f i d = " 2 1 7 " > 0 0 w h i c h t h e y a r e m e a s u r e d . P r o d u c t i o n m a n a g e r s h a v e / p > p b d s f i d = " 2 1 8 " > 0 0 a l w a y s s e e n t h e i r j o b a s o n e o f m i n i m i z i n g c o s t s a n d / p >。
新教材同步备课2024春高中生物第3章基因的本质3.3DNA的复制课件新人教版必修2
(2)注意碱基的单位是“对”还是“个”。 (3)切记在DNA复制过程中,无论复制了几次,含有亲代脱氧 核苷酸单链的DNA分子都只有两个。 (4)看清试题中问的是“DNA分子数”还是“链数”,“含” 还是“只含”等关键词,以免掉进陷阱。
二、DNA分子的复制
例1.某DNA分子中含有1 000个碱基对(被32P标记),其中有胸腺 嘧啶400个。若将该DNA分子放在只含被31P标记的脱氧核苷酸的 培养液中让其复制两次,子代DNA分子相对分子质量平均比原来 减少 1 500 。
F2:
提出DNA离心
高密度带 低密度带 高密度带
低密度带 高密度带
一、DNA复制的推测—— 假说-演绎法
1.提出问题 2.提出假说
(1)演绎推理 ③分散复制
15N 15N
提出DNA离心
P:
3.验证假说
15N 14N
F1:
细胞分 裂一次
转移到含 14NH4Cl的培养 液中
提出DNA离心
细胞再 分裂一次
二、DNA分子的复制
例3.若亲代DNA分子经过诱变,某位点上一个正常碱基变成了5-溴 尿嘧啶(BU),诱变后的DNA分子连续进行2次复制,得到4个子 代DNA分子如图所示,则BU替换的碱基可能是( C )
A.腺嘌呤 C.胞嘧啶
B.胸腺嘧啶或腺嘌呤 D.鸟嘌呤或胞嘧啶
二、DNA分子的复制
例4. 5-BrU(5-溴尿嘧啶)既可以与A配对,又可以与C配对。将一 个正常的具有分裂能力的细胞,接种到含有A、G、C、T、5-BrU 五种核苷酸的适宜培养基上,至少需要经过几次复制后,才能实现 细胞中某DNA分子某位点上碱基对从T—A到G—C的替换( B )
高效液相色谱法测定鸡肉中磺胺类药物残留的不确定度评定_赵健亚
高效液相色谱法测定鸡肉中磺胺类药物残留的不确定度评定赵健亚1,陈 丹2,谢怀根2,肖 静1,*(1.南通大学公共卫生学院,江苏 南通 226019;2.南通市动物疫病预防控制中心,江苏 南通 226006) 摘 要:依据JJF 1059—1999《测量不确定度评定与表示》和CNAS —GL06《化学分析中不确定度的评估指南》规定的测量不确定度的基本方法,建立数学模型,分析高效液相色谱法测定鸡肉中磺胺类药物残留的不确定度来源,并对各分量进行量化,给出5种磺胺类药物残留测定结果的扩展不确定度,评定结果表明:影响检测结果不确定度的主要因素为标准曲线拟合、样品前处理过程和测量重复性等。
关键词:不确定度;磺胺;高效液相色谱法;鸡肉Uncertainty Evaluation for Determination of Sulfonamide Residues in Chicken by High Performance Liquid ChromatographyZHAO Jian-ya 1,CHEN Dan 2,XIE Huai-gen 2,XIAO Jing 1,*(1. School of Public Health, Nantong University, Nantong 226019, China ;2. Nantong Center for Animal Disease Control and Prevention, Nantong 226006, China)Abstract :A mathematical model was established according to the basic method for uncertainty evaluation from JJF 1059—1999 and CNAS-GL06. The sources of uncertainty for determining sulfonamide residues in chicken by high performance liquid chromatography (HPLC) were analyzed and each component of uncertainty was quantified. The expanded uncertainty for fi ve sulfonamides residues results was calculated. The major uncertainty sources were standard curve fi tting, sample pretreatment and measurement repeatability.Key words :uncertainty ;sulfonamides ;high performance liquid chromatography (HPLC);chicken 中图分类号:TS207.3 文献标志码:A 文章编号:1002-6630(2013)10-0144-04doi:10.7506/spkx1002-6630-201310031收稿日期:2012-02-24基金项目:国家自然科学基金青年科学基金项目(31000539);南通市应用研究计划项目(K2010066)作者简介:赵健亚(1980—),女,讲师,硕士,主要从事食品安全质量控制研究。
Algebraic spin liquid as the mother of many competing orders
spinons is natural, they do not behave as free quasiparticles even at asymptotically low energy. Furthermore, the dynamic critical exponent z = 1. Alternatively, the longdistance, low-energy properties are controlled by an interacting, conformally invariant fixed point. Such states were dubbed algebraic spin liquids (ASL) in Ref. 7. Here we show that, remarkably, several competing orders are unified within the sF state by an emergent SU (4) symmetry, and all have the same slowly-varying long-distance correlations. Two of the competing orders are simply the the N´ eel vector, and the order parameter for the columnar and box valence-bond solid (VBS) states – cartoon pictures of these orders are shown in Fig. 1. To be precise, con-
Algebraic spin liquid as the mother of many competing orders
2020GRE填空题目答案及精选解析
2020GRE填空题目答案及精选解析GRE填空题目答案及解析:隐居的教士4. The reclusive clergyman may have lived and died in melancholy, but this doesn’t seem to have (i)___ his genius in any way. On the contrary, we find ourselves wondering whether his genius wasn’t (ii)___ in some mysterious way by his mood.Blank (i)Blank (ii)A. influencedD. servedB. hamperedE. controlledC. triggeredF. identified选 BD翻译:隐居的教士也许一生忧郁寡欢,但这丝毫没影响他的天才。
相反,我们好奇的是,是否正是他的忧郁以某种神秘的方式激发了他的天才。
reclusive 隐居的,单独的,隔离的clergyman 教士或牧师hamper 妨碍trigger 引发,触发,使爆炸whether … wasn't 这种用法我没见过,不过我把whether 理解为whether … or not 的省略形式,所以后面肯定否定都不影响whether 的双指向。
认为我理解错的了同学或老师,请指导我,谢谢。
GRE填空题目答案及解析:考古学遗址7. The Chavez Pass archaeological site was initially interpreted as indicative of ___ society, since it was thought to have been at the center of a cluster of smaller, contemporarysettlements that it presumably controlled.A. an expansionistB. a hierarchicalC. an urbanD. a heterogeneousE. a diverseF. a stratified选 BF翻译:CP 考古学遗址最开始被理解为代表了某种等级制社会,因为它被认为处于一系列同时代的,可视为由它控制的较小的殖民地中心位置。
专八英语阅读
英语专业八级考试TEM-8阅读理解练习册(1)(英语专业2012级)UNIT 1Text AEvery minute of every day, what ecologist生态学家James Carlton calls a global ―conveyor belt‖, redistributes ocean organisms生物.It’s planetwide biological disruption生物的破坏that scientists have barely begun to understand.Dr. Carlton —an oceanographer at Williams College in Williamstown,Mass.—explains that, at any given moment, ―There are several thousand marine species traveling… in the ballast water of ships.‖ These creatures move from coastal waters where they fit into the local web of life to places where some of them could tear that web apart. This is the larger dimension of the infamous无耻的,邪恶的invasion of fish-destroying, pipe-clogging zebra mussels有斑马纹的贻贝.Such voracious贪婪的invaders at least make their presence known. What concerns Carlton and his fellow marine ecologists is the lack of knowledge about the hundreds of alien invaders that quietly enter coastal waters around the world every day. Many of them probably just die out. Some benignly亲切地,仁慈地—or even beneficially — join the local scene. But some will make trouble.In one sense, this is an old story. Organisms have ridden ships for centuries. They have clung to hulls and come along with cargo. What’s new is the scale and speed of the migrations made possible by the massive volume of ship-ballast water压载水— taken in to provide ship stability—continuously moving around the world…Ships load up with ballast water and its inhabitants in coastal waters of one port and dump the ballast in another port that may be thousands of kilometers away. A single load can run to hundreds of gallons. Some larger ships take on as much as 40 million gallons. The creatures that come along tend to be in their larva free-floating stage. When discharged排出in alien waters they can mature into crabs, jellyfish水母, slugs鼻涕虫,蛞蝓, and many other forms.Since the problem involves coastal species, simply banning ballast dumps in coastal waters would, in theory, solve it. Coastal organisms in ballast water that is flushed into midocean would not survive. Such a ban has worked for North American Inland Waterway. But it would be hard to enforce it worldwide. Heating ballast water or straining it should also halt the species spread. But before any such worldwide regulations were imposed, scientists would need a clearer view of what is going on.The continuous shuffling洗牌of marine organisms has changed the biology of the sea on a global scale. It can have devastating effects as in the case of the American comb jellyfish that recently invaded the Black Sea. It has destroyed that sea’s anchovy鳀鱼fishery by eating anchovy eggs. It may soon spread to western and northern European waters.The maritime nations that created the biological ―conveyor belt‖ should support a coordinated international effort to find out what is going on and what should be done about it. (456 words)1.According to Dr. Carlton, ocean organism‟s are_______.A.being moved to new environmentsB.destroying the planetC.succumbing to the zebra musselD.developing alien characteristics2.Oceanographers海洋学家are concerned because_________.A.their knowledge of this phenomenon is limitedB.they believe the oceans are dyingC.they fear an invasion from outer-spaceD.they have identified thousands of alien webs3.According to marine ecologists, transplanted marinespecies____________.A.may upset the ecosystems of coastal watersB.are all compatible with one anotherC.can only survive in their home watersD.sometimes disrupt shipping lanes4.The identified cause of the problem is_______.A.the rapidity with which larvae matureB. a common practice of the shipping industryC. a centuries old speciesD.the world wide movement of ocean currents5.The article suggests that a solution to the problem__________.A.is unlikely to be identifiedB.must precede further researchC.is hypothetically假设地,假想地easyD.will limit global shippingText BNew …Endangered‟ List Targets Many US RiversIt is hard to think of a major natural resource or pollution issue in North America today that does not affect rivers.Farm chemical runoff残渣, industrial waste, urban storm sewers, sewage treatment, mining, logging, grazing放牧,military bases, residential and business development, hydropower水力发电,loss of wetlands. The list goes on.Legislation like the Clean Water Act and Wild and Scenic Rivers Act have provided some protection, but threats continue.The Environmental Protection Agency (EPA) reported yesterday that an assessment of 642,000 miles of rivers and streams showed 34 percent in less than good condition. In a major study of the Clean Water Act, the Natural Resources Defense Council last fall reported that poison runoff impairs损害more than 125,000 miles of rivers.More recently, the NRDC and Izaak Walton League warned that pollution and loss of wetlands—made worse by last year’s flooding—is degrading恶化the Mississippi River ecosystem.On Tuesday, the conservation group保护组织American Rivers issued its annual list of 10 ―endangered‖ and 20 ―threatened‖ rivers in 32 states, the District of Colombia, and Canada.At the top of the list is the Clarks Fork of the Yellowstone River, whereCanadian mining firms plan to build a 74-acre英亩reservoir水库,蓄水池as part of a gold mine less than three miles from Yellowstone National Park. The reservoir would hold the runoff from the sulfuric acid 硫酸used to extract gold from crushed rock.―In the event this tailings pond failed, the impact to th e greater Yellowstone ecosystem would be cataclysmic大变动的,灾难性的and the damage irreversible不可逆转的.‖ Sen. Max Baucus of Montana, chairman of the Environment and Public Works Committee, wrote to Noranda Minerals Inc., an owner of the ― New World Mine‖.Last fall, an EPA official expressed concern about the mine and its potential impact, especially the plastic-lined storage reservoir. ― I am unaware of any studies evaluating how a tailings pond尾矿池,残渣池could be maintained to ensure its structural integrity forev er,‖ said Stephen Hoffman, chief of the EPA’s Mining Waste Section. ―It is my opinion that underwater disposal of tailings at New World may present a potentially significant threat to human health and the environment.‖The results of an environmental-impact statement, now being drafted by the Forest Service and Montana Department of State Lands, could determine the mine’s future…In its recent proposal to reauthorize the Clean Water Act, the Clinton administration noted ―dramatically improved water quality since 1972,‖ when the act was passed. But it also reported that 30 percent of riverscontinue to be degraded, mainly by silt泥沙and nutrients from farm and urban runoff, combined sewer overflows, and municipal sewage城市污水. Bottom sediments沉积物are contaminated污染in more than 1,000 waterways, the administration reported in releasing its proposal in January. Between 60 and 80 percent of riparian corridors (riverbank lands) have been degraded.As with endangered species and their habitats in forests and deserts, the complexity of ecosystems is seen in rivers and the effects of development----beyond the obvious threats of industrial pollution, municipal waste, and in-stream diversions改道to slake消除the thirst of new communities in dry regions like the Southwes t…While there are many political hurdles障碍ahead, reauthorization of the Clean Water Act this year holds promise for US rivers. Rep. Norm Mineta of California, who chairs the House Committee overseeing the bill, calls it ―probably the most important env ironmental legislation this Congress will enact.‖ (553 words)6.According to the passage, the Clean Water Act______.A.has been ineffectiveB.will definitely be renewedC.has never been evaluatedD.was enacted some 30 years ago7.“Endangered” rivers are _________.A.catalogued annuallyB.less polluted than ―threatened rivers‖C.caused by floodingD.adjacent to large cities8.The “cataclysmic” event referred to in paragraph eight would be__________.A. fortuitous偶然的,意外的B. adventitious外加的,偶然的C. catastrophicD. precarious不稳定的,危险的9. The owners of the New World Mine appear to be______.A. ecologically aware of the impact of miningB. determined to construct a safe tailings pondC. indifferent to the concerns voiced by the EPAD. willing to relocate operations10. The passage conveys the impression that_______.A. Canadians are disinterested in natural resourcesB. private and public environmental groups aboundC. river banks are erodingD. the majority of US rivers are in poor conditionText CA classic series of experiments to determine the effects ofoverpopulation on communities of rats was reported in February of 1962 in an article in Scientific American. The experiments were conducted by a psychologist, John B. Calhoun and his associates. In each of these experiments, an equal number of male and female adult rats were placed in an enclosure and given an adequate supply of food, water, and other necessities. The rat populations were allowed to increase. Calhoun knew from experience approximately how many rats could live in the enclosures without experiencing stress due to overcrowding. He allowed the population to increase to approximately twice this number. Then he stabilized the population by removing offspring that were not dependent on their mothers. He and his associates then carefully observed and recorded behavior in these overpopulated communities. At the end of their experiments, Calhoun and his associates were able to conclude that overcrowding causes a breakdown in the normal social relationships among rats, a kind of social disease. The rats in the experiments did not follow the same patterns of behavior as rats would in a community without overcrowding.The females in the rat population were the most seriously affected by the high population density: They showed deviant异常的maternal behavior; they did not behave as mother rats normally do. In fact, many of the pups幼兽,幼崽, as rat babies are called, died as a result of poor maternal care. For example, mothers sometimes abandoned their pups,and, without their mothers' care, the pups died. Under normal conditions, a mother rat would not leave her pups alone to die. However, the experiments verified that in overpopulated communities, mother rats do not behave normally. Their behavior may be considered pathologically 病理上,病理学地diseased.The dominant males in the rat population were the least affected by overpopulation. Each of these strong males claimed an area of the enclosure as his own. Therefore, these individuals did not experience the overcrowding in the same way as the other rats did. The fact that the dominant males had adequate space in which to live may explain why they were not as seriously affected by overpopulation as the other rats. However, dominant males did behave pathologically at times. Their antisocial behavior consisted of attacks on weaker male,female, and immature rats. This deviant behavior showed that even though the dominant males had enough living space, they too were affected by the general overcrowding in the enclosure.Non-dominant males in the experimental rat communities also exhibited deviant social behavior. Some withdrew completely; they moved very little and ate and drank at times when the other rats were sleeping in order to avoid contact with them. Other non-dominant males were hyperactive; they were much more active than is normal, chasing other rats and fighting each other. This segment of the rat population, likeall the other parts, was affected by the overpopulation.The behavior of the non-dominant males and of the other components of the rat population has parallels in human behavior. People in densely populated areas exhibit deviant behavior similar to that of the rats in Calhoun's experiments. In large urban areas such as New York City, London, Mexican City, and Cairo, there are abandoned children. There are cruel, powerful individuals, both men and women. There are also people who withdraw and people who become hyperactive. The quantity of other forms of social pathology such as murder, rape, and robbery also frequently occur in densely populated human communities. Is the principal cause of these disorders overpopulation? Calhoun’s experiments suggest that it might be. In any case, social scientists and city planners have been influenced by the results of this series of experiments.11. Paragraph l is organized according to__________.A. reasonsB. descriptionC. examplesD. definition12.Calhoun stabilized the rat population_________.A. when it was double the number that could live in the enclosure without stressB. by removing young ratsC. at a constant number of adult rats in the enclosureD. all of the above are correct13.W hich of the following inferences CANNOT be made from theinformation inPara. 1?A. Calhoun's experiment is still considered important today.B. Overpopulation causes pathological behavior in rat populations.C. Stress does not occur in rat communities unless there is overcrowding.D. Calhoun had experimented with rats before.14. Which of the following behavior didn‟t happen in this experiment?A. All the male rats exhibited pathological behavior.B. Mother rats abandoned their pups.C. Female rats showed deviant maternal behavior.D. Mother rats left their rat babies alone.15. The main idea of the paragraph three is that __________.A. dominant males had adequate living spaceB. dominant males were not as seriously affected by overcrowding as the otherratsC. dominant males attacked weaker ratsD. the strongest males are always able to adapt to bad conditionsText DThe first mention of slavery in the statutes法令,法规of the English colonies of North America does not occur until after 1660—some forty years after the importation of the first Black people. Lest we think that existed in fact before it did in law, Oscar and Mary Handlin assure us, that the status of B lack people down to the 1660’s was that of servants. A critique批判of the Handlins’ interpretation of why legal slavery did not appear until the 1660’s suggests that assumptions about the relation between slavery and racial prejudice should be reexamined, and that explanation for the different treatment of Black slaves in North and South America should be expanded.The Handlins explain the appearance of legal slavery by arguing that, during the 1660’s, the position of white servants was improving relative to that of black servants. Thus, the Handlins contend, Black and White servants, heretofore treated alike, each attained a different status. There are, however, important objections to this argument. First, the Handlins cannot adequately demonstrate that t he White servant’s position was improving, during and after the 1660’s; several acts of the Maryland and Virginia legislatures indicate otherwise. Another flaw in the Handlins’ interpretation is their assumption that prior to the establishment of legal slavery there was no discrimination against Black people. It is true that before the 1660’s Black people were rarely called slaves. But this shouldnot overshadow evidence from the 1630’s on that points to racial discrimination without using the term slavery. Such discrimination sometimes stopped short of lifetime servitude or inherited status—the two attributes of true slavery—yet in other cases it included both. The Handlins’ argument excludes the real possibility that Black people in the English colonies were never treated as the equals of White people.The possibility has important ramifications后果,影响.If from the outset Black people were discriminated against, then legal slavery should be viewed as a reflection and an extension of racial prejudice rather than, as many historians including the Handlins have argued, the cause of prejudice. In addition, the existence of discrimination before the advent of legal slavery offers a further explanation for the harsher treatment of Black slaves in North than in South America. Freyre and Tannenbaum have rightly argued that the lack of certain traditions in North America—such as a Roman conception of slavery and a Roman Catholic emphasis on equality— explains why the treatment of Black slaves was more severe there than in the Spanish and Portuguese colonies of South America. But this cannot be the whole explanation since it is merely negative, based only on a lack of something. A more compelling令人信服的explanation is that the early and sometimes extreme racial discrimination in the English colonies helped determine the particular nature of the slavery that followed. (462 words)16. Which of the following is the most logical inference to be drawn from the passage about the effects of “several acts of the Maryland and Virginia legislatures” (Para.2) passed during and after the 1660‟s?A. The acts negatively affected the pre-1660’s position of Black as wellas of White servants.B. The acts had the effect of impairing rather than improving theposition of White servants relative to what it had been before the 1660’s.C. The acts had a different effect on the position of white servants thandid many of the acts passed during this time by the legislatures of other colonies.D. The acts, at the very least, caused the position of White servants toremain no better than it had been before the 1660’s.17. With which of the following statements regarding the status ofBlack people in the English colonies of North America before the 1660‟s would the author be LEAST likely to agree?A. Although black people were not legally considered to be slaves,they were often called slaves.B. Although subject to some discrimination, black people had a higherlegal status than they did after the 1660’s.C. Although sometimes subject to lifetime servitude, black peoplewere not legally considered to be slaves.D. Although often not treated the same as White people, black people,like many white people, possessed the legal status of servants.18. According to the passage, the Handlins have argued which of thefollowing about the relationship between racial prejudice and the institution of legal slavery in the English colonies of North America?A. Racial prejudice and the institution of slavery arose simultaneously.B. Racial prejudice most often the form of the imposition of inheritedstatus, one of the attributes of slavery.C. The source of racial prejudice was the institution of slavery.D. Because of the influence of the Roman Catholic Church, racialprejudice sometimes did not result in slavery.19. The passage suggests that the existence of a Roman conception ofslavery in Spanish and Portuguese colonies had the effect of _________.A. extending rather than causing racial prejudice in these coloniesB. hastening the legalization of slavery in these colonies.C. mitigating some of the conditions of slavery for black people in these coloniesD. delaying the introduction of slavery into the English colonies20. The author considers the explanation put forward by Freyre andTannenbaum for the treatment accorded B lack slaves in the English colonies of North America to be _____________.A. ambitious but misguidedB. valid有根据的but limitedC. popular but suspectD. anachronistic过时的,时代错误的and controversialUNIT 2Text AThe sea lay like an unbroken mirror all around the pine-girt, lonely shores of Orr’s Island. Tall, kingly spruce s wore their regal王室的crowns of cones high in air, sparkling with diamonds of clear exuded gum流出的树胶; vast old hemlocks铁杉of primeval原始的growth stood darkling in their forest shadows, their branches hung with long hoary moss久远的青苔;while feathery larches羽毛般的落叶松,turned to brilliant gold by autumn frosts, lighted up the darker shadows of the evergreens. It was one of those hazy朦胧的, calm, dissolving days of Indian summer, when everything is so quiet that the fainest kiss of the wave on the beach can be heard, and white clouds seem to faint into the blue of the sky, and soft swathing一长条bands of violet vapor make all earth look dreamy, and give to the sharp, clear-cut outlines of the northern landscape all those mysteries of light and shade which impart such tenderness to Italian scenery.The funeral was over,--- the tread鞋底的花纹/ 踏of many feet, bearing the heavy burden of two broken lives, had been to the lonely graveyard, and had come back again,--- each footstep lighter and more unconstrained不受拘束的as each one went his way from the great old tragedy of Death to the common cheerful of Life.The solemn black clock stood swaying with its eternal ―tick-tock, tick-tock,‖ in the kitchen of the brown house on Orr’s Island. There was there that sense of a stillness that can be felt,---such as settles down on a dwelling住处when any of its inmates have passed through its doors for the last time, to go whence they shall not return. The best room was shut up and darkened, with only so much light as could fall through a little heart-shaped hole in the window-shutter,---for except on solemn visits, or prayer-meetings or weddings, or funerals, that room formed no part of the daily family scenery.The kitchen was clean and ample, hearth灶台, and oven on one side, and rows of old-fashioned splint-bottomed chairs against the wall. A table scoured to snowy whiteness, and a little work-stand whereon lay the Bible, the Missionary Herald, and the Weekly Christian Mirror, before named, formed the principal furniture. One feature, however, must not be forgotten, ---a great sea-chest水手用的储物箱,which had been the companion of Zephaniah through all the countries of the earth. Old, and battered破旧的,磨损的, and unsightly难看的it looked, yet report said that there was good store within which men for the most part respect more than anything else; and, indeed it proved often when a deed of grace was to be done--- when a woman was suddenly made a widow in a coast gale大风,狂风, or a fishing-smack小渔船was run down in the fogs off the banks, leaving in some neighboring cottage a family of orphans,---in all such cases, the opening of this sea-chest was an event of good omen 预兆to the bereaved丧亲者;for Zephaniah had a large heart and a large hand, and was apt有…的倾向to take it out full of silver dollars when once it went in. So the ark of the covenant约柜could not have been looked on with more reverence崇敬than the neighbours usually showed to Captain Pennel’s sea-chest.1. The author describes Orr‟s Island in a(n)______way.A.emotionally appealing, imaginativeB.rational, logically preciseC.factually detailed, objectiveD.vague, uncertain2.According to the passage, the “best room”_____.A.has its many windows boarded upB.has had the furniture removedC.is used only on formal and ceremonious occasionsD.is the busiest room in the house3.From the description of the kitchen we can infer that thehouse belongs to people who_____.A.never have guestsB.like modern appliancesC.are probably religiousD.dislike housework4.The passage implies that_______.A.few people attended the funeralB.fishing is a secure vocationC.the island is densely populatedD.the house belonged to the deceased5.From the description of Zephaniah we can see thathe_________.A.was physically a very big manB.preferred the lonely life of a sailorC.always stayed at homeD.was frugal and saved a lotText BBasic to any understanding of Canada in the 20 years after the Second World War is the country' s impressive population growth. For every three Canadians in 1945, there were over five in 1966. In September 1966 Canada's population passed the 20 million mark. Most of this surging growth came from natural increase. The depression of the 1930s and the war had held back marriages, and the catching-up process began after 1945. The baby boom continued through the decade of the 1950s, producing a population increase of nearly fifteen percent in the five years from 1951 to 1956. This rate of increase had been exceeded only once before in Canada's history, in the decade before 1911 when the prairies were being settled. Undoubtedly, the good economic conditions of the 1950s supported a growth in the population, but the expansion also derived from a trend toward earlier marriages and an increase in the average size of families; In 1957 the Canadian birth rate stood at 28 per thousand, one of the highest in the world. After the peak year of 1957, thebirth rate in Canada began to decline. It continued falling until in 1966 it stood at the lowest level in 25 years. Partly this decline reflected the low level of births during the depression and the war, but it was also caused by changes in Canadian society. Young people were staying at school longer, more women were working; young married couples were buying automobiles or houses before starting families; rising living standards were cutting down the size of families. It appeared that Canada was once more falling in step with the trend toward smaller families that had occurred all through theWestern world since the time of the Industrial Revolution. Although the growth in Canada’s population had slowed down by 1966 (the cent), another increase in the first half of the 1960s was only nine percent), another large population wave was coming over the horizon. It would be composed of the children of the children who were born during the period of the high birth rate prior to 1957.6. What does the passage mainly discuss?A. Educational changes in Canadian society.B. Canada during the Second World War.C. Population trends in postwar Canada.D. Standards of living in Canada.7. According to the passage, when did Canada's baby boom begin?A. In the decade after 1911.B. After 1945.C. During the depression of the 1930s.D. In 1966.8. The author suggests that in Canada during the 1950s____________.A. the urban population decreased rapidlyB. fewer people marriedC. economic conditions were poorD. the birth rate was very high9. When was the birth rate in Canada at its lowest postwar level?A. 1966.B. 1957.C. 1956.D. 1951.10. The author mentions all of the following as causes of declines inpopulation growth after 1957 EXCEPT_________________.A. people being better educatedB. people getting married earlierC. better standards of livingD. couples buying houses11.I t can be inferred from the passage that before the IndustrialRevolution_______________.A. families were largerB. population statistics were unreliableC. the population grew steadilyD. economic conditions were badText CI was just a boy when my father brought me to Harlem for the first time, almost 50 years ago. We stayed at the hotel Theresa, a grand brick structure at 125th Street and Seventh avenue. Once, in the hotel restaurant, my father pointed out Joe Louis. He even got Mr. Brown, the hotel manager, to introduce me to him, a bit punchy强力的but still champ焦急as fast as I was concerned.Much has changed since then. Business and real estate are booming. Some say a new renaissance is under way. Others decry责难what they see as outside forces running roughshod肆意践踏over the old Harlem. New York meant Harlem to me, and as a young man I visited it whenever I could. But many of my old haunts are gone. The Theresa shut down in 1966. National chains that once ignored Harlem now anticipate yuppie money and want pieces of this prime Manhattan real estate. So here I am on a hot August afternoon, sitting in a Starbucks that two years ago opened a block away from the Theresa, snatching抓取,攫取at memories between sips of high-priced coffee. I am about to open up a piece of the old Harlem---the New York Amsterdam News---when a tourist。
绿色化学与化工导论Chapter 3 atom economy
3、Less Hazardous Chemical Synthesis
Wherever practicable, synthetic methods should be designed to use and generate substances that possess little or no toxicity to people or the environment.
Chapter 3
Principles and Concepts of Green Chemistry
MIKE LANCASTER
• Introduction • Sustainable Development and Green Chemistry • Atom Economy • Atom Economic Reactions
12、Inherently Safer Chemistry for Accident Prevention
Substances and the form of a substance used in a chemical process should be chosen to minimise the potential for chemical accidents, including releases, explosions, and fires.
4、Designing Safer Chemicals
Chemical products should be designed to effect their desired function while minimising their toxicity.
5、Safer Solvents and Auxiliaries
精子冻存
Hindawi Publishing CorporationAdvances in UrologyVolume2012,Article ID854837,12pagesdoi:10.1155/2012/854837Review ArticleHuman Sperm Cryopreservation:Update on Techniques,Effect on DNA Integrity,and Implications for ARTMarlea Di Santo,Nicoletta Tarozzi,Marco Nadalini,and Andrea BoriniTecnobios Procreazione,Centre for Reproductive Health,Via Dante15,40125Bologna,ItalyCorrespondence should be addressed to Marlea Di Santo,disanto@tecnobiosprocreazione.itReceived5August2011;Revised22September2011;Accepted27September2011Academic Editor:James A.BrownCopyright©2012Marlea Di Santo et al.This is an open access article distributed under the Creative Commons Attribution License,which permits unrestricted use,distribution,and reproduction in any medium,provided the original work is properly cited.Cryopreservation of human spermatozoa—introduced in the1960’s—has been recognized as an efficient procedure for management of male fertility before therapy for malignant diseases,vasectomy or surgical infertility treatments,to store donor and partner spermatozoa before assisted reproduction treatments and to ensure the recovery of a small number of spermatozoa in severe male factor infertility.Despite the usefulness of it,cryopreservation may lead to deleterious changes of sperm structure and function:while the effects of cryopreservation on cells are well documented,to date there is no agreement in the literature on whether or not cryopreservation affects sperm chromatin integrity or on the use of a unique and functional protocol for the freezing-thawing procedure.Therefore,sperm cryopreservation is an important component of fertility management and much of its successful application seems to affect the reproductive outcome of assisted reproduction technologies(ART):appropriate use of cryoprotectants before and sperm selection technologies after cryopreservation seem to have the greatest impact on preventing DNA fragmentation,thus improving sperm cryosurvival rates.1.IntroductionThe procedure that makes it possible to stabilize the cells at cryogenic temperatures is called cryopreservation,also known as an applied aspect of cryobiology or the study of life at low temperatures.Many advances in the cryopreservation technology have led to the development of methods that allow for low-temperature maintenance of a variety of cell types including male and female gametes,small multicel-lular organisms,and even more complex organisms such as embryos.Cryopreservation of human spermatozoa—introduced in the1960’s[1]—has overcome many space and time limitations and now forms integral part of assisted reproduction technologies(ARTs).This technique becomes particularly important in cases of preservation of male fertility before radiotherapy or chemotherapy[2]which may lead to testicular failure or ejaculatory dysfunction.In fact,semen cryostorage seems to be the only proven method that may offer these couples a chance of having children in the future:cancer therapy could in fact lead to damage,resulting in subfertility or sterility due to gonad removal or permanent damage to germ cells caused by adjuvant therapy.In particular,the risk associated to therapy depends on several factors:the age of the patient at the time of treatment,the dose, site,and type of treatment[3].Also some nonmalignant diseases,such as diabetes and autoimmune disorders,may lead to testicular damage.Cryopreservation is advisable also in these conditions[4].In countries in which heterologous fertilization is allowed by law and in donor insemination programmes cryopreservation is necessary to have enough time to screen donors for infectious agents,such as the human immunodeficiency(HIV)and hepatitis B viruses, before the cryopreserved semen is used for clinical purposes [5].In azoospermic patients,who have undergone testic-ular sperm extraction or percutaneous epididymal sperm aspiration,sperm cryostorage is also used to avoid repeated biopsies or aspirations[6].Furthermore,cryopreservation is routinely performed in patients who—having to start an assisted reproduction treatment—decide to preemptively freeze the semen sample to avoid inconveniences due to failed ejaculation often associated with“semen collection stress,”certain emotional states,or other commitments at the time of oocyte retrieval[7].Finally,male gamete freezing is largelyrecommended to preserve fertility in those subjects who—for one reason or another—are exposed to potentially toxic agents which may interfere with gametogenesis[7].2.Techniques for CryopreservationThere are two main conventional freezing techniques used in sperm cryopreservation:slow freezing and rapid freezing 2.1.Slow Freezing.The slow freezing technique proposed by Behrman and Sawada[8]consists of progressive sperm cooling over a period of2–4h in two or three steps, either manually or automatically using a semiprogrammable freezer.The manual method is performed by simultaneously decreasing the temperature of the semen while adding a cryoprotectant in a stepwise manner and after plunging the samples into liquid nitrogen[9].It has been shown that the optimal initial cooling rate of the specimen from room temperature to5◦C is0.5–1◦C/min[10].The sample is then frozen from5◦C to−80◦C at a rate of1–10◦C/min.The specimen is then plunged into liquid nitrogen at−196◦C [11].In spite of reports showing successful sperm freezing with manual techniques,the reproducibility of this procedure could pose some problems.For this reason,programmable freezers have been investigated[12].The freezers use a plate to hold the straws;these are cooled by liquid nitrogen held in a storage tank under the plate.Liquid nitrogen is poured into the tank,and the machine,once programmed, uses the software data logging to obtain cooling from20◦C to−80◦C at rate of1.5◦C/min and then at6◦C/min;at completion of the freezing the straws are removed and stored into liquid nitrogen at−196◦C.This takes about 40min[12].Programmes are simple to use and allow for a cooling combination which does not require continuous operator intervention and have been used to increase the reproducibility of the freezing operations[12].Some authors argue that conventional slow freezing, either manual or automated,causes extensive chemical-phys-ical damage to the sperm probably because of ice crystalliza-tion[13].2.2.Rapid Freezing.Rapid freezing wasfirst proposed by Sherman[14].This technique requires direct contact between the straws and the nitrogen vapours for8–10min and immersion in liquid nitrogen at−196◦C.Inside nitrogen vapours there is a thermal gradient,as a function of the distance and the volume of the liquid below.The sample is initially mixed in dropwise manner with equal volume of cold cryoprotectant;the mixture is loaded into the straws and left to incubate at4◦C for10minutes.The straws are then placed at a distance of15–20cm above the level of liquid nitrogen(−80◦C)for15min;after this stage,the straws are immersed in liquid nitrogen.During cooling it is preferable to place the straws in horizontal position to minimize the heat difference between the two ends.This technique has some drawbacks among which;for example,low reproducibility,indeed,the temperature drop curve cannot be controlled,and the freezing temperatures may vary from−70,−80,and−99◦C[7].2.3.Cryopreservation of Small Numbers of Spermatozoa.The conventional methods of sperm cryopreservation described above are not ideal to cryopreserve small numbers of cells, such as epididymal and testicular spermatozoa.Efficient cry-opreservation of surgically retrieved spermatozoa,as men-tioned earlier in this chapter,reduces the number of surgical interventions and avoids the logistic problem associated with coordinating the women’s oocyte retrieval and also the risk of no sperm being found on the day of oocyte retrieval[15]. Thus,novel cryopreservation approaches have been designed to cryopreserve limited numbers of motile sperms in a very small volume(Table1).Both biological and nonbiological carriers have been tried for the cryopreservation of low numbers of spermatozoa,although,to date,no prospective randomised trials have been conducted to demonstrate that any single carrier is superior to the others[15].Furthermore, to date there is a limited use of these technologies in the majority of IVF programs.This suggests that novel cryopreservation technology,designed to handle small sperm numbers and needs to be further explored[15].Whatever the method used,to obtain good results it is essential to correctly perform all the steps before and after cryopreservation:the choice of more suitable cryoprotectants and the thawing procedure are particularly important.2.4.Cryoprotectants.Cryoprotectants are low-molecular-weight and highly permeable chemicals used to protect spermatozoa from freeze damage by ice crystallization.There are four main well-known cryoprotectants:glycerol,ethylene glycol,dimethyl sulphoxide,and1,2-propanediol.Cryopro-tectants act by decreasing the freezing point of a substance, reducing the amount of salts and solutes present in the liquid phase of the sample and by decreasing ice formation within the spermatozoa[16].Usually the cryoprotectants are added in an equal volume of semen in a dropwise manner,gently mixed at room temperature,and then placed at37◦C for10–15minutes to allow for proper equilibration between the cells and the medium[7].It is necessary that the medium interacts with the cells.Indeed,the effectiveness of cryoprotecting substances is also a function of the time of interaction between the cryoprotectants and the cells[7].Glycerol is the permeating cryoprotectant most widely used for human sperm acting on:the membrane structure,permeability and stability of the lipid bilayer,the association of surface proteins and the cellular metabolism. Its employment gives an unfavorable outcome on membrane and acrosome structure,although allowing the freezing of poor quality sperm[7].Sherman’s[14]studies showed that the use of glycerol may cause few alterations such as:presence of an undulating membrane,alteration in acrosomal internal membrane,nucleus inhomogeneity and disorganization in mitochondrial crests.Following this observations other protective substances were proposed such as the dimethyl sulfoxide(DMSO),which has deleterious effects on humanTable1:Approaches to cryopreserve limited number of spermatozoa. CryopreservationtechniquesAuthors Principle Main advantages Main disadvantagesEmpty zona pellucidaBorini et al.[69]Storage of individualspermatozoa in animal orhuman empty zonapellucida.Avoid waste of time inscreening to locate motilesperm;cryoprotectants canbe added and removedwithout loss ofspermatozoa sequestered inthe zonaRisk of biologicalcontamination Cohen et al.[70]Walmsley et al.[71]Montag et al.[72]Hsieh et al.[73]Liu et al.[74]Levi-Setti et al.[75]Cesana et al.[76]Hassa et al.[77]Microdroplets Gil-Salom et al.[78]Storage of droplets ofsperm/cryoprotectantsmixture on the surface ofdry ice and directly plungedinto liquid nitrogenAvoid sperm loss throughadherence to the vesselRisk of cross-contamination;shape and size of dishes makedifficult to handle and store inconventional freezers andliquid nitrogen tanksSereni et al.[79]Quintans et al.[80]Bouamama et al.[81]ICSI pipette Gvakharia et al.[82]Storage of spermatozoa inICSI pipettesSterile,simple,andconvenient systemNot practical for long-termstorage;fragility of ICSIpipettes;risk ofcross-contamination Sohn et al.[83]Volvox globator spheres Just et al.[84]Storage of sperm intospheres of Volvox globatorSignificant postthawrecovery of motile spermExposure to genetic materialfrom the algae;constantsource of algaeAlginate beads Herrler et al.[85]Microencapsulation inalginate beadsInert nature of alginatebeadsDecrease sperm motility withencapsulationCryoloop Nawroth et al.[86]Individual spermatozoadeposited directly oncryoprotectantfilmcovering the nylon loopand immersed in liquidnitrogenExcellent vessel forvitrification;no additionalpreparationOpen system:risk ofcross-contamination Schuster et al.[87]Isachenko et al.[42]Isachenko et al.[42]Desai et al.[88]Desai et al.[89]Agarose microspheres Isaev et al.[90]Storage of sperm loaded inagarose microspheresNonbiological carrierClinical value of this approachnot evaluatedStrawsDesai et al.[91]Sperm/cryoprotectantsloaded into the ministrawSterile,simple,andconvenient systemNot ideal for severely impairedspecimens;sperm loss due toadherence to the vessel Isachenko et al.[92]Koscinski et al.[93]sperm when used at4◦C,and the1,2-propanediol slightly used in sperm cryopreservation[7].2.5.Thawing Procedure.The thawing procedure is an equally important step:the cell must be allowed to recover its normal biological activities trying to avoid abrupt thermal changes as far as possible.Generally speaking,the cryopreservation protocols use a thawing temperature of37◦C;even if higher thawing temperatures allow for more rapid heating,they are not used because of the risks associated to cell damage.At the present time,several thawing techniques are used, among which are(i)thawing at room temperature for10min and subse-quent thermostat pass at37◦C for another10min, (ii)thawing in a thermostat and water-bath at37◦C for 10min,(iii)thawing at room temperature for15min.Once the semen is thawed,it is separated from the cryo-preservation medium by washing in culture medium and centrifuging[7].3.Detrimental Effects of Cryopreservation on Sperm IntegrityCompared with other cell types,spermatozoa seem to be less sensitive to cryopreservation damage because of the high fluidity of the membrane and the low water content(about 50%).Despite this,cryopreservation may lead to deleterious changes of sperm structure and function[17].It was largely reported that several damaging processes could occur during freezing-thawing of human spermatozoa,such as thermal shock with formation of intracellular and extracellular ice crystals,cellular dehydration,and osmotic shock[18].The primary cause of cellular damage during cryopreser-vation is the formation of intracellular or extracellular ice crystals.During the freezing process,the cooling rate plays an important role in determining the extent of cryoinjury to the spermatozoa[9].A rapid cooling rate causes severe intracellular ice formation,since the efflux of water across the membrane is impaired,thus,inducing supercooling.Ice crystals formed breach the membranes and affect the organelle function.This condition leads to impaired cell survival.On the other hand,a too slow cooling rate deter-mines the efflux of water from the internal to the external environment,increasing the concentration of solutes and the osmotic pressure.This condition leads to cell volume changes associated with the movement of water,dehydration, and toxicity damage due to high solute concentration[9]. Cryoinjury is not limited to the freezing process but may also occur during the thawing process as the ice melts or recrystallizes[9].The phenomenon of recrystallization of both intracellular and extracellular ice,in frozen samples, occurs as smaller ice crystals with a rate of recrystallization that increases with increasing temperature[13].It has largely been reported that chilling injury can modify the structure and integrity of plasma membranes [19,20]mainly composed by phospholipids and cholesterol [21].Even though high concentrations of cholesterol and polyunsaturated fatty acids give morefluidity to the mem-brane at lower temperature[22],during cryopreservation the cooling process causes phase transition of membrane lipids and impairs membrane protein function.In particular,the outer layer of the spermatozoal plasma membrane consists of a glycocalyx,a carbohydrate-rich zone that mainly contains oligosaccharide chains that bind to the integral protein of the plasma membrane(glycoproteins)or lipids(glycolipids) [23].Generally,cryopreservation may have a detrimental effect changing the carbohydrate composition of the glyco-calyx,thus impairing the function of membrane proteins which are responsible for ion transport and metabolism and affecting the fertilizing ability[24]:the glycocalyx is involved in some physiological functions such as immune protection of the female genital tract[25],acrosomal reaction[26],and early gamete interaction.The plasma and mitochondrial membranes have the same susceptibility to cryopreservation[27].Mitochondria are placed along the midpiece between the plasma mem-brane and the ninefibrous columns,to form a coating that provides energy necessary for sperm motility[27]. The greatest amount of energy is provided by molecules of ATP synthesized either by glycolysis in the cytoplasm [28]or through oxidative phosphorylation(oxphos)in the mitochondria[10].The ATP generated by oxphos in the inner mitochondrial membrane is transferred to the microtubules,to drive motility[29].Therefore,an impairment of mitochondrial activity may explain the reduction in motility[27].An alteration in mitochondrial membranefluidity may also lead to an alteration in mitochondrial membrane potential and release of ROS[9].The peroxidative damage induced by increased concentration of ROS is associated with damage to the sperm plasma membrane and impairment of the axonemal structure[30].In addition,cryopreservation has been shown to diminish the antioxidant activity of the spermatozoa making them more susceptible to ROS damage [31].High concentration of ROS and fall of antioxidant enzymes lead to cell apoptosis[32].In this context the apoptosis cascade is mediated by activation of the BCL2 family proteins:there is a permeabilization of the outer mito-chondrial membrane through the Bax and BAK proteins and the release of cytochrome[33].In turn,caspase9is activated along with APAF-1to form an apoptosome[34].The release of apoptosis-inducing factors from the mitochondria leads to DNA fragmentation[35].Several studies examined the role of in vitro antioxidant supplementation in protecting the sperm DNA from oxidative damage.For example,when added to the seminalfluid during cryopreservation,genistein [36],resveratrol[37],and ascorbic acid[37]seem to reduce DNA damage;on the contrary,vitamin E[38],ascorbate,and catalase[39]seem to improve motility and reduce ROS levels, though they do not improve spermatozoal viability and do not reduce DNA damage.In any case,the number of these studies and the number of patients they include is still too limited to draw any conclusions on the efficacy of antioxidant supplementation in protecting DNA from freezing-induced damage.3.1.Cryopreservation and DNA Damage.While the effects of cryopreservation on the fertilization capacity,motility,mor-phology,and viability of spermatozoa are well documented, still open is the question of the possible alteration of sperm DNA integrity after freezing-thawing procedures.There is no agreement in the literature neither on whether cryopreserva-tion induces DNA damage nor on the amount of damage.In some studies,authors have reported significant alterations of sperm DNA integrity after cryopreservation/thawing[6,40], whereas other studies have expressed a different opinion [41,42].This controversy between one study and the other couldfirst of all be explained by the fact that thefindings do not refer to a considerable number of samples and is also due to the use of(1)different freezing procedures, (2)different tests to evaluate the DNA integrity,and(3) different semen preparation techniques before cryopreser-vation(i.e.,swimup or density gradient centrifugation). For example,Donnelly and colleagues[6]investigated pre-cryopreservation and postcryopreservation DNA integrity of both semen and prepared sperm samples(density gradient centrifugation or direct swimup)in50men.They reported that freezing sperm in seminal plasma improves postthaw DNA integrity:sperm-frozen unprepared in seminalfluid seems to be more resistant to freezing damage than frozen prepared sperm;further improvement can be achieved by preparing sperm and freezing after readdition of seminal plasma.This may be due to the presence of abundant antioxidants in seminal plasma.Concerning the variability linked to different freezing procedures,in the study of Petym and colleagues[43],the authors evaluated cryodamage on sperm chromatin comparing two different procedures:liquid nitrogen vapour versus computerized program freezer. They analyzed50semen samples using acridine orange and concluded that DNA damage was significantly higher following freezing with liquid nitrogen.From a detailed analysis of the references currently available in the literature,it was found that there are basically three different lines of thought about the question:“Does the freezing-thawing procedure induce sperm DNA damage?”.According to several authors the answer is“YES”(Table2(a)).Spano and his group[44]reported that overall sperm quality deteriorates after freezing-thawing,including sperm DNA integrity assessed by SCSA in19samples.These findings have been confirmed in a study by De Paula and colleagues[40]on77patients,where the authors have eval-uated the degree of sperm DNA fragmentation by TUNEL assay before and after cryopreservation:the authors stressed that the freezing-thawing procedure negatively affects DNA integrity.Furthermore,from the data published in the literature,it is also clear that among the authors who argue that cryopreservation induces sperm DNA damage there is sometimes no agreement on the mechanism which actually induces that damage.For example,in spite of the fact that ROS was widely reported to play an important role in the pathophysiology of damage to human spermatozoa,includ-ing DNA fragmentation,Zribi and his group[45]stated that there is no relationship between DNA fragmentation and DNA oxidation.They suggested that cryopreservation induces sperm DNA fragmentation through other pathways beside oxidative stress,such as defects in DNA repairing enzymes or enhancement of defects already present in sperm cells.This hypothesis is controverted by Thomson and colleagues[46]:despite the use of the same technique to assess DNA oxidation,fluorescent assay for the detection of8oxoguanine,they reported that human sperm DNA fragmentation is associated with an increase in oxidative stress during cryopreservation,rather than the activation of caspase and apoptosis.About the question“Does the freezing-thawing proce-dure induce sperm DNA damage?”some authors follow another line of thought and answer“YES,but with some conditions”(Table2(b)).They support the hypothesis of less susceptibility to freezing damage in the spermatozoa of fertile men,classified using WHO criteria,than those of infertile men.In the study of Donnelly and colleagues [6],semen samples were obtained from17fertile and40 infertile men,and sperm integrity was assessed before and after cryopreservation using the Comet assay.The authors showed that semen from fertile men appears to be more resistant to freezing damage than sample from infertile men; moreover,in fertile man,there was no significant decrease in DNA integrity after cryopreservation.These results support the observation that spermatozoa from infertile men have a greater incidence of irregular chromatin organization and show significantly decreased resistance to thermal denatu-ration compared with spermatozoa from fertile men[47, 48].In fact,as a consequence of reduced protamination, poor-quality spermatozoa often contain partially decon-densed chromatin that generates functional immaturity[6]. Chromatin condensation is fundamental for spermatozoa since spermatogenesis results in the discarding of cytoplasm, leaving the spermatozoa incapable of undertaking DNA repair.According to this hypothesis,Kalthur and colleagues [49],evaluating sperm morphology and sperm DNA damage before and after cryopreservation,reported that the suscep-tibility of morphologically abnormal sperm to DNA damage during the freezing process is significantly higher than that of sperm with normal morphology.They hypothesised that sperm with head abnormalities may have altered membrane physical properties and thereby have altered tolerance to cold stress.However,there are no studies conducted to assess whether or not a morphologically abnormal sperm can retain its chromatin integrity during cryopreservation.In opposition to these two answers to the above question, there is a third line of thought:some authors say:“NO, the freezing-thawing procedure does not compromise sperm DNA integrity”(Table2(c)).For example,Duru and his group[41]evaluated the effects of cryopreservation on DNA fragmentation and membrane integrity in21patients using the TUNEL assay and annexin V.Their results indicated that cryopreservation altered plasma membrane symmetry and was associated with translocation of phosphatidylserine, while DNA integrity was maintained.In addition,Isachenko and colleagues[42],comparing the effects of slow freezing and vitrification on sperm DNA integrity in the absence of cryoprotectant,found that the integrity of DNA is unaffected by cryopreservation.The lack of effects of cryopreservation on sperm DNA has also been confirmed by data of Paasch and colleagues[50].They demonstrated that cryopreser-vation was significantly associated with disruption of the mitochondrial membrane potential,as well as activation of caspase3,8,and9,but no significant changes were observed in DNA fragmentation,as assessed by the TUNEL assay in84 samples.Even if the opinions on the issue of“cryopreservation and DNA damage”are still highly controversial,the evaluation of the impact of cryopreservation on sperm chromatin is of extreme importance.Likewise,sperm DNA integrity is an important factor for the success of ART[51–53].4.Cryopreservation and Reproductive Outcome Cryopreservation is widely known to raise impaired sperm motility and decrease fertilization rate through detrimental effects on membranes,acrosomal structure,and acrosin activity[54].The freezing-thawing procedure of human spermatozoa may also be detrimental to the chromatin structure[55],leading to a potential risk of decondensation of the sperm nucleus after injection into the oocyte,thus, reducing fertilization rate[56].However,a cumulative effect of cryopreservation on sperm fertilization capacity is not definitely established.Considering the decrease in sperm fertilization power induced by cryopreservation,it can be easily understood that intrauterine insemination and conventional in vitro fertilization(IVF)with frozen-thawed spermatozoa result in lower pregnancy rates compared with insemination with fresh sperm[1]:this is the reason why cryopreservation of semen samples before intrauterine insemination or conventional IVF is not recommended.Table2:(a)–(c)Evaluation of DNA integrity after cryopreservation:description of the experimental design and conclusions.(a)Authors Test to evaluate DNA integrity Number of samples Cryopreservationmethod“Does thefreezing-thawingprocedure inducesperm DNA damage?”Hamamah et al.[94]Acridine orange staining andFeulgen-DNA quantitativemicrospectrophotometry10Unspecified YesSpan`o et al.[44]SCSA+Acridine orange staining19Equilibration at37◦C,freezing in liquidnitrogen vapour at−80◦C and then storagein liquid nitrogen at–196◦CYesHammadeh et al.[95]Acridine orange staining59Computerizedslow-stage freezer+static liquid nitrogenvapourYesDonnelly et al.[6]COMET assay40Equilibration at37◦C,freezing in liquidnitrogen vapour at−80◦C and then storagein liquid nitrogen at–196◦CYesGandini et al.[96]Acridine orange staining19Equilibration at37◦C,freezing in liquidnitrogen vapour at−80◦C and then storagein liquid nitrogen at–196◦CYesde Paula et al.[40]TUNEL assay77:(i)30normozoospermic(ii)47oligozoospermicUse of freezer at–20◦C,freezing in liquidnitrogen vapour,thenstorage in liquidnitrogen–196◦CYesPetyim and Choavaratana[43]Acridine orange staining50Freezing with liquidnitrogen vapour+computerized programfreezerYesNagamwuttiwong and Kunathikom[97]Acridine orange staining20Freezing with liquidnitrogen vapourYesDejarkom and Kunathikom[98]Acridine orange staining20Computerizedcontrolled rate freezingYesThomson et al.[46]TUNEL assay60Use of programmablefreezerYesThomson et al.[46]TUNEL assay320Sample frozen with andwithout cryoprotectantby slow-controlled-ratemethod using aprogrammable freezerYesZribi et al.[45]TUNEL assay15Equilibration at37◦C,freezing in liquidnitrogen vapour at−80◦C,then storage inliquid nitrogen at–196◦CYes。
Random Matrices and the Anderson Model
Random Matrices and Wigner’s law
The GUE
The simplest ensemble of random matrices is the Gaussian unitary ensemble. It is a probability measure on random hermitian N × N complex matrices. Each coefficient in the upper triangle of the matrix is identically independently ¯ ji , Gaussian distributed. Here the matrix is H = Hij , H = H ∗ , hence Hij = H and 1 N P (H ) = exp(− TrH ∗ H ) (2.1) Z 2 Z being a normalization factor. The matrix H is made therefore of N (N − 1)/2 complex variables Hij with i < j and N real ones Hii , so there are N 2 real random variables in H . Since
In the GUE, eigenvalues are not independent but tend to ”repel” each other. This is seen in the following behavior of the 2-level correlation R2 R2 (s) = δ (s) + 1 − sin2 πs , (πs)2 (2.11)
觉知及其反身性结构_论意识的现象本性(中国社会科学)
觉知及其反身性结构论意识的现象本性李恒威摘 要:意识作为一种确定的现象,具有明确的特性和品质。
意识现象不但迥异于物理、化学、生命现象,而且与一般的认知和心智现象相比,也有迥然不同的特性。
澄清意识的现象本性,须从与之相关的形而上学和方法论问题入手,由 视角二元论 立场出发,采用对比的方法论原则,并将其置于与一般生物现象既存在差别又连续的背景中来理解。
差异性对比分析表明,意识首要的现象学特性是觉知,觉知是加之于心智之上的独特成分,具有自觉知的反身性结构。
光是理解意识现象本性的一个恰当且强有力的隐喻。
关键词:意识 现象本性 觉知 反身性 对比认识论原则作者李恒威,哲学博士,浙江大学哲学系、浙江大学语言与认知研究中心副教授(杭州 310028)。
一、引 言近代科学自建立以来,以独特的方法研究过各种各样的现象(物理的、化学的、生物的),但对意识这个最为人们所熟知的现象,却直到很晚才介入,这是一个非常令科学尴尬的现象。
正如科赫(Ch Koch)所评价:这些亲知的、主观的体验(ex perience)给在许多其他领域一直证明是如此富有成果的科学方法提出了一个令它畏缩的挑战。
科学能描述大爆炸后几微秒的事件。
它对物质以及如何操控它们提供了日益详尽的解释,并且揭示了大脑及其病理的生物物理和神经生理的细节。
然而,这个同样的方法目前却不能对第一人称的、主观的体验如何适合于物理的、客观的宇宙提供一个满意的解释。
意识这个 残酷的 事实是作为一个全然的惊异闯入[科学世界]的,它似乎并不是由任何传统的物理学或生物学现象产生的。
自然科学如此之晚才介入意识研究,存在两个方面的原因,其一是 外在的 ,其二是 内在的 。
外在的 是由于研究意识的科学手段和方法直到最近才发展起来。
埃德尔曼(G M Edelman)和托诺尼(G T ononi)在 意识的宇宙 物质如何转变为精神 (2000)中写道: 意识是哲学探讨的主要对象之一,只是到了最近人们才将意识纳入到用科学实验来研究本文为国家社会科学基金项目(项目编号:09BZX023)成果。
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¯p = ǫp dN
−∞
|f (p) |2
dp . 2π
(4)
Clearly, the total number of particles and the field total energy in the state (1) are infinite if the function |f (p) |2 decreases too slowly at large momenta. In particular, the integrals in Eqs. (3, 4) diverge for the case f (p) = c = const. When dealing with the Unruh problem it is convenient to quantize the field in the basis of eigenfunctions Ψκ (x), x = (t, z ), of the Lorentz boost operator rather than in the plane-wave basis [2,4]. These functions have the following integral representation, see, e.g., [4] Ψκ (x) = 1 23/2 π
∞
∗ †
E-mail: belinski@icra.it E-mail: narozhny@theor.mephi.ru ‡ E-mail: a fedotov@ 1 We use natural units c = = kB = 1
dq eim(z sinh q−t cosh q)−iκq ,
′ [bκ , b† κ′ ] = δ (κ − κ ), † [bκ , bκ′ ] = [b† κ , b κ′ ] = 0 .
M,
(7)
(10)
where b† 0 is the creation operator of a particle in the state corresponding to the singular zero boost mode Ψ0 (x), √ (8) Ψ0 (x) = −i 2∆(+) (x; m) , with ∆(+) (x; m) being the positive-frequency Whightman function, see Ref. [4]. Thus, in the framework of the boost quantization scheme the state (1) with f (p) = const contains an infinite number of particles concentrated in a single quantum state, namely in the state with zero value of the boost quantum number κ. Hence, in a sense, this state is equivalent to the Bose-Einstein condensate. It is well known that the Whightman function [which determines the zero mode Eq. (8)] vanishes exponentially fast for large spacelike distances away from the light cone. It is clear therefore that particles of the condensate are located basically inside the light cone. Moreover, the energy density in this state is infinite at the light cone. This is closely related to singular properties of the zero boost mode. Indeed, near the light cone x+ x− → 0 (x± = t ± z ) the zero mode Ψ0 (x) behaves [4] as 1 Ψ0 (x) ∼ − √ ln[−m2 x+ x− + iε sgn (t)] , 2 2π (9)
where a† p is a conventional creation operator of a particle in the plane wave state with momentum p and energy ǫp = p2 + m2 , f (p) is a complex weight function, ¯ ¯ is the Zcoh = eN is the normalization constant and N expectation value of total number of particles. The momentum distribution of particles in the state (1) is described by ¯p = coh | a† ap | coh dp = dN p |f (p) |2 dp , 2πǫp (2)
−∞
(5)
1
and boost destruction operators bκ can be expressed in terms of plane wave operators ap as
∞
bκ =
−∞
dp 2πǫp
ǫp + p m
iκ
ap .
(6)
The two quantization schemes are unitary equivalent [4]. Let us introduce the notation | c for the coherent state (1) with f (p) = c = const. Using Eq. (6) it can be easily represented in terms of boost operators as
N.B. Narozhny† , A.M. Fedotov‡ , and V.D. Mur,
Moscow Engineering Physics Institute, 115409 Moscow, Russia
arXiv:hep-th/0306191v1 19 Jun 2003
We have shown that the Unruh quantization scheme can be realized in Minkowski spacetime in the presence of BoseEinstein condensate containing infinite average number of particles in the zero boost mode and located basically inside the light cone. Unlike the case of an empty Minkowski spacetime the condensate provides the boundary conditions necessary for the Fulling quantization of the part of the field restricted only to the Rindler wedge of Minkowski spacetime. 03.70.+k, 04.70.Dy
dp f (p) a† |0 p 2πǫp
M,
(1)
¯ of the total number of while the expectation values N ¯ in the state (1) are particles and of the field energy E given by Eqs. (3, 4) respectively
scalar field | coh =−1/2 Z来自ohexp
∞
−∞
The Unruh effect [1,2] consists of the statement that any detector uniformly accelerated in the empty Minkowski space (MS) reveals a universal response as if it were emersed into a thermal bath with the temperature TDU = g/2π , where g is the proper acceleration of the detector1 . The universality of the detector response is based on interpretation of the Minkowski vacuum state in terms of Fulling-Unruh particles states which appear in the Unruh quantization scheme [2] alternative to the standard plane wave quantization (see also Ref. [3] and citations therein). However, we have shown in our earlier paper [4], see also Refs. [5,6], that the Unruh construction [2] was not a valid quantization scheme in the empty MS. This conclusion stems from the fact that the Unruh scheme neglects the contribution of the singular zero boost mode to the total field amplitude. The purpose of this paper is to present a situation for which the Unruh quantization procedure could be realized. We argue that this is the case for MS which is not empty but filled with a background of special type. We hope that our consideration will clarify the drawbacks of the Unruh approach to the problem in empty MS. For the sake of simplicity we will restrict our consideration to two-dimensional case. Let us consider a coherent state for a massive neutral