常见分子实验限制性内切酶酶切位点大全
常见限制性酶切位点
常见限制性内切酶识别序列(酶切位点)（BamHI、EcoRI、HindIII、NdeI、XhoI等）分子生物学实验室技术2009-11-17 17:46:32 阅读2235 评论2 字号：大中小订阅.在分子克隆实验中，限制性内切酶是必不可少的工具酶。
无论是构建克隆载体还是表达载体，要根据载体选择合适的内切酶（当然，使用T载就不必考虑了）。
先将引物设计好，然后添加酶切识别序列到引物5’端（英语怎么说？）。
常用的内切酶比如BamHI、EcoRI、HindIII、NdeI、XhoI等可能你都已经记住了它们的识别序列，不过为了保险起见，还是得查证一下。
下面，我就总结了一些常用的内切酶的识别序列，仅供各位参考。
下面这些内切酶都属于II型内切酶。
先介绍一下什么是II型内切酶吧。
The Type II restriction systems typically contain individual restriction enzymes and modification enzymes encoded by separate genes. The Type II restriction enzymes typically recognize specific DNA sequences and cleave at constant positions at or close to that sequence to produce 5-phosphates and 3-hydroxyls. Usually they require Mg 2+ ions as a cofactor, although some have more exotic requirements. The methyltransferases usually recognize the same sequence although some are more promiscuous. Three types of DNA methyltransferases have been found as part of Type II R-M systems forming either C5-methylcytosine, N4-methylcytosine or N6-methyladenine.ApaI （类型：Type II restriction enzyme ）识别序列：5'GGGCC^C 3'BamHI（类型：Type II restriction enzyme ）识别序列：5' G^GATCC 3'BglII （类型：Type II restriction enzyme ）识别序列：5' A^GATCT 3'EcoRI （类型：Type II restriction enzyme ）识别序列：5' G^AA TTC 3'HindIII （类型：Type II restriction enzyme ）识别序列：5' A^AGCTT 3'KpnI （类型：Type II restriction enzyme ）识别序列：5' GGTAC^C 3'NcoI （类型：Type II restriction enzyme ）识别序列：5' C^CA TGG 3'NdeI （类型：Type II restriction enzyme ）识别序列：5' CA^TATG 3'NheI （类型：Type II restriction enzyme ）识别序列：5' G^CTAGC 3'NotI （类型：Type II restriction enzyme ）识别序列：5' GC^GGCCGC 3'SacI （类型：Type II restriction enzyme ）识别序列：5' GAGCT^C 3'SalI （类型：Type II restriction enzyme ）识别序列：5' G^TCGAC 3'SphI （类型：Type II restriction enzyme ）识别序列：5' GCA TG^C 3'XbaI （类型：Type II restriction enzyme ）识别序列：5' T^CTAGA 3'XhoI （类型：Type II restriction enzyme ）识别序列：5' C^TCGAG 3'当然，上面总结的这些肯定不全，要查找更多内切酶的识别序列，你还可以选择下面几种方法：1. 查你所使用的内切酶的公司的目录或者网站；NEB网站上提供的识别序列图表下载2. 用软件如：Primer Premier5.0或Bioedit等，这些软件均提供了内切酶识别序列的信息；3. 推荐到NEB的REBASE数据库去查（网址：/rebase/rebase.html）当你设计好引物，添加上了内切酶识别序列，下一步或许是添加保护碱基了，可以参考：NEB公司网站提供的保护碱基参考表下载NEB公司网站上关于设计PCR引物保护碱基的参考双酶切buffer的选择（MBI、罗氏、NEB、Promega、Takara）这里再给大家推荐一种新的不需要连接反应的分子克隆方法，优点包括：①设计引物不必考虑选择什么酶切位点；②不必考虑保护碱基的问题；③不必每次都选择合适的酶来酶切质粒制备载体；④而且不需要DNA连接酶；⑤假阳性几率低（因为没有连接反应这一步，载体自连的问题没有了）。
酶切位点汇总
酶切位点汇总
酶切位点，又称为限制性内切酶位点，是指DNA分子上特定的序列，这些序列是限制
性内切酶可以识别和切割的地方。
限制性内切酶是一种在细菌和其它生物中广泛存在的酶，能够切割或切除一个或多个DNA碱基对。
这些限制性内切酶在生物技术领域广泛应用，用
于DNA序列分析、DNA重组、基因工程等方面。
以下是常见的几种酶切位点：
1. EcoRI切割位点是5′-GAATTC-3′，这是一种广泛应用的限制性内切酶，通常用于DNA纯化、制备DNA载体等。
2. BamHI切割位点是5′-GGATCC-3′，BamHI能够切割链间，产生具有黏性末端的DNA 序列。
常被用于制备双链DNA的黏性末端。
4. PstI切割位点是5′-CTGCAG-3′，PstI是一种双切酶，可以切割成不同长度的DNA 序列，适用于构建多种不同长度的DNA分子。
总之，酶切位点及其对应的限制性内切酶在现代生物领域有着广泛的应用和重要的作用。
了解不同的酶切位点是有很大帮助的，它可以为实验设计和分子生物学研究提供基础。
同时，也让我们更好地理解限制性内切酶在DNA分子上的作用，帮助我们在生物技术领域
更加熟练地掌握其应用。
限制性内切酶酶切位点汇总
限制性内切酶酶切位点汇AatII识别位点Acc65I识别位点AccI识别位点AciI识别位点AclI识别位点AcuI识别位点AfeI识别位点AflII识别位点AflIII识别位点AgeI识别位点AhdI识别位点AleI识别位点AluI识别位点AlwI识别位点AlwNI识别位点ApaI识别位点ApaLI识别位点ApeKI识别位点ApoI识别位点AscI识别位点AseI识别位点AsiSI识别位点AvaI识别位点AvaII识别位点AvrII识别位点BaeI识别位点BamHI识别位点BanI识别位点BanII识别位点BbsI识别位点BbvCI识别位点BbvI识别位点BccI识别位点BceAI识别位点BcgI识别位点BciVI识别位点BclI识别位点BfaI识别位点BfuAI识别位点BglI识别位点BglII识别位点BlpI识别位点Bme1580I识别位点BmgBI识别位点BmrI识别位点BmtI识别位点BpmI识别位点Bpu10I识别位点BpuEI识别位点BsaAI识别位点BsaBI识别位点BsaHI识别位点BsaI识别位点BsaJI识别位点BsaWI识别位点BsaXI识别位点BseRI识别位点BseYI识别位点BsgI识别位点BsiEI识别位点BsiHKAI识别位点BsiWI识别位点BslI识别位点BsmAI识别位点BsmBI识别位点BsmFI识别位点BsmI识别位点BsoBI识别位点Bsp1286I识别位点BspCNI识别位点BspDI识别位点BspEI识别位点BspHI识别位点BspMI识别位点BspQI识别位点BsrBI识别位点BsrDI识别位点BsrFI识别位点BsrGI识别位点BsrI识别位点BssHII识别位点BssKI识别位点BssSI识别位点BstAPI识别位点BstBI识别位点BstEII识别位点BstNI识别位点BstUI识别位点BstXI识别位点BstYI识别位点BstZ17I识别位点Bsu36I识别位点BtgI识别位点BtgZI识别位点BtsCI识别位点BtsI识别位点Cac8I识别位点ClaI识别位点CspCI识别位点CviAII识别位点CviKI-1识别位点CviQI识别位点DdeI识别位点DpnI识别位点DpnII识别位点DraI识别位点DraIII识别位点DrdI识别位点EaeI识别位点EaeI识别位点EagI识别位点EarI识别位点EciI识别位点EcoNI识别位点EcoO109I识别位点EcoP15I识别位点EcoRI识别位点EcoRV识别位点FatI识别位点FauI识别位点Fnu4HI识别位点FokI识别位点FseI识别位点FspI识别位点HaeII识别位点HaeIIIHgaI识别位点HhaI识别位点HincII识别位点HindIII识别位点HinfI识别位点HinP1I识别位点HpaI识别位点HpaII识别位点HphI识别位点Hpy188I识别位点Hpy188III识别位点Hpy99I识别位点HpyAV识别位点HpyCH4III识别位点HpyCH4IV HpyCH4V识别位点KasI识别位点KpnI识别位点MboI识别位点MboII识别位点MfeI识别位点MluI识别位点MlyI识别位点MmeI识别位点MnlI识别位点MscI识别位点MseI识别位点MslI识别位点MspA1I识别位点MspI识别位点MwoI识别位点NaeI识别位点NarI识别位点NciI识别位点NcoI识别位点NdeI识别位点NgoMIV识别位点NheI识别位点NlaIII识别位点NlaIV识别位点NmeAIII识别位点NotI识别位点NruI识别位点NsiI识别位点NspI识别位点PacI识别位点PaeR7I识别位点PciI识别位点PflFI识别位点PflMI识别位点PhoI识别位点PleI识别位点PmeI识别位点PmlI识别位点PpuMI识别位点PshAI识别位点PsiI识别位点PspGI识别位点PspOMI识别位点PspXI识别位点PstI识别位点PvuI识别位点PvuII识别位点RsaI识别位点RsrII识别位点SacI识别位点SacII识别位点SalI识别位点SapI识别位点Sau3AI识别位点Sau96I识别位点SbfI识别位点ScaI识别位点ScrFI识别位点SexAI识别位点SfaNI识别位点SfcI识别位点SfiI识别位点SfoI识别位点SgrAI识别位点SmaI识别位点SmlI识别位点SnaBI识别位点SpeI识别位点SphI识别位点SspI识别位点StuI识别位点StyD4I识别位点StyI识别位点SwaI识别位点TaqαI识别位点TfiI识别位点TliI识别位点TseI识别位点Tsp45I识别位点Tsp509I识别位点TspMI识别位点TspRI识别位点Tth111I识别位点XbaI识别位点XcmI识别位点XhoI识别位点XmaI识别位点XmnI识别位点ZraI识别位点。
限制性内切酶酶切位点汇总
限制性内切酶酶切位点汇总限制性内切酶（Restriction Endonuclease）是一类存在于细菌体内的酶，它能够识别特定的酶切位点，并在该位点上切割DNA链。
限制性内切酶起源于细菌，原本作为细菌对抗噬菌体感染的防御机制，但现在被广泛应用于分子生物学和基因工程领域。
限制性内切酶的分类和命名依据它们发现的第一个类型的噬菌体。
如EcoRI是从大肠杆菌中分离出的内切酶，与T4噬菌体相关；HindIII是与T4噬菌体有关的内切酶等。
酶名中的缩写首字母通常是酶切位点的首字母，比如EcoRI是指E.coli的RY粘性末端的切点。
现在限制性内切酶已被发现有超过3000多个类型。
下面是一些常见的限制性内切酶的切割位点汇总：1. EcoRI：切割位点为G↓AATTC（↓表示切割位点），产生的切割后的两个DNA片段是G-AATTC和CTTAA-G。
2. HindIII：切割位点为A↓AGCTT，产生的切割后的两个DNA片段是A-AGCTT和TTCGA-A。
3. SmaI：切割位点为CCC↓GGG，产生的切割后的两个DNA片段是CCC-GGG和GGG-CCC。
4. BamHI：切割位点为G↓GATCC，产生的切割后的两个DNA片段是G-GATCC和CCTAG-G。
5. XhoI：切割位点为C↓TCGAG，产生的切割后的两个DNA片段是C-TCGAG和GAGCT-C。
6. NotI：切割位点为GC×GGCCGC，产生的切割后的两个DNA片段是GC-GGCCGC和CGC-GC。
7. EcoRV：切割位点为GAT↓ATC，产生的切割后的两个DNA片段是GAT-ATC和TAC-TAG。
8. KpnI：切割位点为GGTAC↓C，产生的切割后的两个DNA片段是GGTAC-C和CCATG-G。
9. SalI：切割位点为G↓TCGAC，产生的切割后的两个DNA片段是G-TCGAC和CAGCT-G。
10. PstI：切割位点为CTGCA↓G，产生的切割后的两个DNA片段是CTGC-AG和GACG-T。
常用限制性内切酶酶切位点总结
常用限制性内切酶酶切位点总结————————————————————————————————作者：————————————————————————————————日期：Acc65I识别位点AccI识别位点AciI识别位点AclI识别位点AcuI识别位点AfeI识别位点AflII识别位点AflIII识别位点AgeI识别位点AhdI识别位点AleI识别位点AluI识别位点AlwI识别位点AlwNI识别位点ApaI识别位点ApaLI识别位点ApeKI识别位点ApoI识别位点AscI识别位点AseI识别位点AsiSI识别位点AvaI识别位点AvaII识别位点AvrII识别位点BaeI识别位点BamHI识别位点BanI识别位点BanII识别位点BbvCI识别位点BbvI识别位点BccI识别位点BceAI识别位点BcgI识别位点BciVI识别位点BclI识别位点BfaI识别位点BfuAI识别位点BglI识别位点BglII识别位点BlpI识别位点Bme1580I识别位点BmgBI识别位点BmrI识别位点BmtI识别位点BpmI识别位点Bpu10I识别位点BpuEI识别位点BsaAI识别位点BsaBI识别位点BsaHI识别位点BsaI识别位点BsaJI识别位点BsaWI识别位点BsaXI识别位点BseRI识别位点BseYI识别位点BsiEI识别位点BsiHKAI识别位点BsiWI识别位点BslI识别位点BsmAI识别位点BsmBI识别位点BsmFI识别位点BsmI识别位点BsoBI识别位点Bsp1286I识别位点BspCNI识别位点BspDI识别位点BspEI识别位点BspHI识别位点BspMI识别位点BspQI识别位点BsrBI识别位点BsrDI识别位点BsrFI识别位点BsrGI识别位点BsrI识别位点BssHII识别位点BssKI识别位点BssSI识别位点BstAPI识别位点BstBI识别位点BstEII识别位点BstNI识别位点BstXI识别位点BstYI识别位点BstZ17I识别位点Bsu36I识别位点BtgI识别位点BtgZI识别位点BtsCI识别位点BtsI识别位点Cac8I识别位点ClaI识别位点CspCI识别位点CviAII识别位点CviKI-1识别位点CviQI识别位点DdeI识别位点DpnI识别位点DpnII识别位点DraI识别位点DraIII识别位点DrdI识别位点EaeI识别位点EaeI识别位点EagI识别位点EarI识别位点EciI识别位点EcoNI识别位点EcoO109I识别位点EcoP15I识别位点EcoRI识别位点EcoRV识别位点FatI识别位点FauI识别位点Fnu4HI识别位点FokI识别位点FseI识别位点FspI识别位点HaeII识别位点HaeIIIHgaI识别位点HhaI识别位点HincII识别位点HindIII识别位点HinfI识别位点HinP1I识别位点HpaI识别位点HpaII识别位点HphI识别位点Hpy188I识别位点Hpy188III识别位点Hpy99I识别位点HpyAV识别位点HpyCH4III识别位点HpyCH4IV HpyCH4V识别位点KasI识别位点KpnI识别位点MboI识别位点MboII识别位点MfeI识别位点MluI识别位点MlyI识别位点MmeI识别位点MnlI识别位点MscI识别位点MseI识别位点MslI识别位点MspA1I识别位点MspI识别位点MwoI识别位点NaeI识别位点NarI识别位点NciI识别位点NdeI识别位点NgoMIV识别位点NheI识别位点NlaIII识别位点NlaIV识别位点NmeAIII识别位点NotI识别位点NruI识别位点NsiI识别位点NspI识别位点PacI识别位点PaeR7I识别位点PciI识别位点PflFI识别位点PflMI识别位点PhoI识别位点PleI识别位点PmeI识别位点PmlI识别位点PpuMI识别位点PshAI识别位点PsiI识别位点PspGI识别位点PspOMI识别位点PspXI识别位点PstI识别位点PvuI识别位点PvuII识别位点RsrII识别位点SacI识别位点SacII识别位点SalI识别位点SapI识别位点Sau3AI识别位点Sau96I识别位点SbfI识别位点ScaI识别位点ScrFI识别位点SexAI识别位点SfaNI识别位点SfcI识别位点SfiI识别位点SfoI识别位点SgrAI识别位点SmaI识别位点SmlI识别位点SnaBI识别位点SpeI识别位点SphI识别位点SspI识别位点StuI识别位点StyD4I识别位点StyI识别位点SwaI识别位点TaqαI识别位点TfiI识别位点TseI识别位点Tsp45I识别位点Tsp509I识别位点TspMI识别位点TspRI识别位点Tth111I识别位点XbaI识别位点XcmI识别位点XhoI识别位点XmaI识别位点XmnI识别位点ZraI识别位点。
限制性内切酶酶切位点汇总
限制性内切酶酶切位点汇AatII识别位点Acc65I识别位点AccI识别位点AciI识别位点AclI识别位点AcuI识别位点AfeI识别位点AflII识别位点AflIII识别位点AgeI识别位点AhdI识别位点AleI识别位点AluI识别位点AlwI识别位点AlwNI识别位点ApaI识别位点ApaLI识别位点ApeKI识别位点ApoI识别位点AscI识别位点AseI识别位点AsiSI识别位点AvaI识别位点AvaII识别位点AvrII识别位点BaeI识别位点BamHI识别位点BanI识别位点BanII识别位点BbsI识别位点BbvCI识别位点BbvI识别位点BccI识别位点BceAI识别位点BcgI识别位点BciVI识别位点BclI识别位点BfaI识别位点BfuAI识别位点BglI识别位点BglII识别位点BlpI识别位点Bme1580I识别位点BmgBI识别位点BmrI识别位点BmtI识别位点BpmI识别位点Bpu10I识别位点BpuEI识别位点BsaAI识别位点BsaBI识别位点BsaHI识别位点BsaI识别位点BsaJI识别位点BsaWI识别位点BsaXI识别位点BseRI识别位点BseYI识别位点BsgI识别位点BsiEI识别位点BsiHKAI识别位点BsiWI识别位点BslI识别位点BsmAI识别位点BsmBI识别位点BsmFI识别位点BsmI识别位点BsoBI识别位点Bsp1286I识别位点BspCNI识别位点BspDI识别位点BspEI识别位点BspHI识别位点BspMI识别位点BspQI识别位点BsrBI识别位点BsrDI识别位点BsrFI识别位点BsrGI识别位点BsrI识别位点BssHII识别位点BssKI识别位点BssSI识别位点BstAPI识别位点BstBI识别位点BstEII识别位点BstNI识别位点BstUI识别位点BstXI识别位点BstYI识别位点BstZ17I识别位点Bsu36I识别位点BtgI识别位点BtgZI识别位点BtsCI识别位点BtsI识别位点Cac8I识别位点ClaI识别位点CspCI识别位点CviAII识别位点CviKI-1识别位点CviQI识别位点DdeI识别位点DpnI识别位点DpnII识别位点DraI识别位点DraIII识别位点DrdI识别位点EaeI识别位点EaeI识别位点EagI识别位点EarI识别位点EciI识别位点EcoNI识别位点EcoO109I识别位点EcoP15I识别位点EcoRI识别位点EcoRV识别位点FatI识别位点FauI识别位点Fnu4HI识别位点FokI识别位点FseI识别位点FspI识别位点HaeII识别位点HaeIIIHgaI识别位点HhaI识别位点HincII识别位点HindIII识别位点HinfI识别位点HinP1I识别位点HpaI识别位点HpaII识别位点HphI识别位点Hpy188I识别位点Hpy188III识别位点Hpy99I识别位点HpyAV识别位点HpyCH4III识别位点HpyCH4IV HpyCH4V识别位点KasI识别位点KpnI识别位点MboI识别位点MboII识别位点MfeI识别位点MluI识别位点MlyI识别位点MmeI识别位点MnlI识别位点MscI识别位点MseI识别位点MslI识别位点MspA1I识别位点MspI识别位点MwoI识别位点NaeI识别位点NarI识别位点NciI识别位点NcoI识别位点NdeI识别位点NgoMIV识别位点NheI识别位点NlaIII识别位点NlaIV识别位点NmeAIII识别位点NotI识别位点NruI识别位点NsiI识别位点NspI识别位点PacI识别位点PaeR7I识别位点PciI识别位点PflFI识别位点PflMI识别位点PhoI识别位点PleI识别位点PmeI识别位点PmlI识别位点PpuMI识别位点PshAI识别位点PsiI识别位点PspGI识别位点PspOMI识别位点PspXI识别位点PstI识别位点PvuI识别位点PvuII识别位点RsaI识别位点RsrII识别位点SacI识别位点SacII识别位点SalI识别位点SapI识别位点Sau3AI识别位点Sau96I识别位点SbfI识别位点ScaI识别位点ScrFI识别位点SexAI识别位点SfaNI识别位点SfcI识别位点SfiI识别位点SfoI识别位点SgrAI识别位点SmaI识别位点SmlI识别位点SnaBI识别位点SpeI识别位点SphI识别位点SspI识别位点StuI识别位点StyD4I识别位点StyI识别位点SwaI识别位点TaqαI识别位点TfiI识别位点TliI识别位点TseI识别位点Tsp45I识别位点Tsp509I识别位点TspMI识别位点TspRI识别位点Tth111I识别位点XbaI识别位点XcmI识别位点XhoI识别位点XmaI识别位点XmnI识别位点ZraI识别位点。
限制性内切酶酶切位点_方便搜索(word版本).
AatII 识别位点GACGTCCTGCAGAcc65I 识别位点GTMKACCAKMTGAccI 识别位点GTMKACCAKMTGAciI 识别位点CCGCGGCGAclI 识别位点AACGTTTTGCAAAcuI 识别位点CTGAAGGACTTCAfeI 识别位点AGCGCTTCGCGAAflII 识别位点CTTAAGGAATTCAflIII 识别位点ACRYGTTGYRCAAgeI 识别位点ACCGGTTGGCCAAhdI 识别位点GACNNNNNGTCCTGNNNNNCAGAleI 识别位点CACNNNNGTGGTGNNNNCACAluI 识别位点AGCTTCGAAlwI 识别位点GGATCCCTAGAlwNI 识别位点CAGNNNCTGGTCNNNGACApaI 识别位点GGGCCCCCCGGGApaLI 识别位点GTGCACCACGTGApeKI 识别位点GCWGCCGWCGApoI 识别位点RAATTYYTTAARAscI 识别位点GGCGCGCCCCGCGCGGAseI 识别位点ATTAATTAATTAAsiSI 识别位点GCGATCGCCGCTAGCGAvaI 识别位点CYCGRGGRGCYCAvaII 识别位点GGWCCCCWGGAvrII 识别位点CCTAGGGGATCCBaeI 识别位点NACNGTAYCNBamHI 识别位点GGATCCCCTAGGBanI 识别位点GGYRCCCCRYGGBanII 识别位点GRGCYCCYCGRGBbsI 识别位点GAAGACCTTCTGBbvCI 识别位点CCTCAGCGGAGTCGBbvI 识别位点GCAGCCGTCGBccI 识别位点 CCATCGGTAGBceAI 识别位点ACGGCTGCCGBcgI 识别位点CGANTGN GCTNACGBciVI 识别位点GTATCCCATAGGBclI 识别位点TGATCAACTACTBfaI 识别位点CTAGGATCBfuAI 识别位点ACCTGCTGGACGBglI 识别位点GCCNNNNNGGCCGGNNNNNCCGBglII 识别位点AGATCTTCTAGABlpI 识别位点GCTNAGCCGANTCGBme1580I 识别位点GKGCMCCMCGKGCACGTCGTGCAGBmrI 识别位点ACTGGGNTGACCCNBmtI 识别位点GCTAGCCGATCGBpmI 识别位点CTGGAGNGACCTCNBpu10I 识别位点CCTNAGCGGANTCGBpuEI 识别位点CTTGAGNGAACTCNBsaAI 识别位点YACGTRRTGCAYBsaBI 识别位点GATNNNNATCCTANNNNTAGBsaHI 识别位点GRCGYC CYGCRGBsaI 识别位点GGTCTCNCCAGAGNBsaJI 识别位点CCNNGGGGNNCCBsaWI 识别位点WCCGGWWGGCCWBsaXI 识别位点NACNCTCCNNTGNGAGGNBseRI 识别位点GAGGAGNCTCCTCNBseYI 识别位点CCCAGCGGGTCGBsgI 识别位点GTGCAGCACGTCBsiEI 识别位点CGRYCGGCYRGCGWGCWCCWCGWGBsiWI 识别位点CGTACGGCATGCBslI 识别位点CCNNNNNNNGGGGNNNNNNNCCBsmAI 识别位点GTCTCNCAGAGNBsmBI 识别位点CGTCTCNGCAGAGNBsmFI 识别位点GGGACCCCTGBsmI 识别位点GAATGCNCTTACGNBsoBI 识别位点CYCGRGGRGCYCBsp1286I 识别位点GDGCHC CHCGDGBspCNI 识别位点CTCAGN GAGTCNBspDI 识别位点ATCGATTAGCTABspEI 识别位点TCCGGAAGGCCTBspHI 识别位点TCATGAAGTACTBspMI 识别位点ACCTGCNTGGACGNBspQI 识别位点GCTCTTCNCGAGAAGNBsrBI 识别位点CCGCTCGGCGAGBsrDI 识别位点GCAATGNNCGTTACNNRCCGGYYGGCCRBsrGI 识别位点TGTACAACATGTBsrI 识别位点ACTGGNTGACCNBssHII 识别位点GCGCGCCGCGCGBssKI 识别位点CCNGGGGNCCBssSI 识别位点CACGAGGTGCTCBstAPI 识别位点GCANNNNNTGCCGTNNNNNACGBstBI 识别位点TTCGAAAAGCTTBstEII 识别位点GGTNACC CCANTGGBstNI 识别位点CCWGGGGWCCBstUI 识别位点CGCGGCGCBstXI 识别位点CCANNNNNNTGGGGTNNNNNNACCBstYI 识别位点RGATCYYCTAGRBstZ17I 识别位点GTATACCATATGBsu36I 识别位点CCTNAGGGGANTCCBtgI 识别位点CCRYGGGGYRCCBtgZI 识别位点GCGATGCGCTACGCAGTGNNCGTCACNNBtsI 识别位点GCAGTGNNCGTCACNNCac8I 识别位点GCNNGCCGNNCGClaI 识别位点ATCGATTAGCTACspCI 识别位点NCAANGTGGNNGTTNCACCNCviAII 识别位点CATGGTACCviKI-1识别位点RGCYYCGRCviQI 识别位点GTACCATGDdeI 识别位点CTNAG GANTCDpnI 识别位点GATCCTAGDpnII 识别位点GATCCTAGDraI 识别位点TTTAAAAAATTTDraIII 识别位点CACNNNGTGGTGNNNCACDrdI 识别位点GACNNNNNNGTCCTGNNNNNNCAGEaeI 识别位点YGGCCRRCCGGYEagI 识别位点CGGCCGGCCGGCEarI 识别位点CTCTTCGAGAAGEciI 识别位点GGCGGACCGCCTEcoNI 识别位点CCTNNNNNAGGGGANNNNNTCCEcoO109I 识别位点RGGNCCYYCCNGGREcoP15I 识别位点CAGCAGGTCGTCEcoRI 识别位点GAATTCCTTAAGEcoRV 识别位点GATATCCTATAGFatI 识别位点CATGGTACFauI 识别位点CCCGCGGGCGFnu4HI 识别位点GCNGCCGBCGFokI 识别位点GGATGNCCTACNFseI 识别位点GGCCGGCCCCGGCCGGFspI 识别位点TGCGCAACGCGTHaeII 识别位点RGCGCYYCGCGRHaeIIIHgaI 识别位点GACGCCTGCGHhaI 识别位点GCGCCGCGHincII 识别位点GTYRACCARYTGHindIII 识别位点AAGCTTTTCGAAHinfI 识别位点GANTCCTNAGHinP1I 识别位点GCGCCGCGHpaI 识别位点GTTAACCAATTGHpaII 识别位点CCGGGGCCHphI 识别位点GGTGACCACTHpy188I 识别位点TCNGAAGNCTHpy188III 识别位点TCNNGAAGNNCTHpy99I 识别位点CGWCG GCWGCHpyAV 识别位点CCTTCGGAAGHpyCH4III 识别位点ACNGTTGCCAHpyCH4IVHpyCH4V 识别位点TGCAACGTKasI 识别位点GGCGCCCCGCGGKpnI 识别位点GGTACCCCATGGMboI 识别位点GATCCTAGMboII 识别位点GAAGACTTCTMfeI 识别位点CAATTGGTTAACACGCGTTGCGCAMlyI 识别位点GAGTCCTCAGMmeI 识别位点TCCRACAGGYTGMnlI 识别位点CCTCGGAGMscI 识别位点TGGCCAACCGGTMseI 识别位点TTAAAATTMslI 识别位点CAYNNNNRTGGTRNNNNYACMspA1I 识别位点CMGCKGGKCGMCMspI 识别位点CCGG GGCCMwoI 识别位点GCNNNNNNNGCCGNNNNNNNCGNaeI 识别位点GCCGCCGGCCGNarI 识别位点GGCGCCCCGCGGNciI 识别位点CCSGGGGSCCNcoI 识别位点CCATGGGGTACCNdeI 识别位点CATATG GTATACNgoMIV识别位点GCCGGC CGGCCGNheI 识别位点GCTAGC CGATCGCATG GTACNlaIV识别位点GGNNCC CCNNGGNmeAIII 识别位点GCCGAG CGGCTCNotI 识别位点GCGGCCGC CGCCGGCGNruI识别位点TCGCGA AGCGCTNsiI 识别位点ATGCAT TACGTANspI 识别位点RCATGY YGTACRPacI 识别位点TTAATTAA AATTAATTPaeR7I 识别位点CTCGAG GAGCTCPciI 识别位点ACATGTTGTACAPflFI 识别位点GACNNNGTCCTGNNNCAGPflMI 识别位点CCANNNNNTGGGGTNNNNNACCPhoI 识别位点GGCCPleI 识别位点GAGTCNCTCAGNPmeI 识别位点GTTTAAACCAAATTTGPmlI 识别位点CACGTGGTGCACPpuMI 识别位点RGGWCCYYCCWGGRGACNNNNGTCCTGNNNNCAGPsiI 识别位点TTATAAAATATTPspGI 识别位点CCWGGGGWCCPspOMI 识别位点GGGCCCCCCGGGPspXI 识别位点VCTCGAGBBGAGCTCVPstI 识别位点CTGCAGGACGTCPvuI 识别位点CGATCGGCTAGCPvuII 识别位点CAGCTGGTCGACRsaI 识别位点GTAC CATGRsrII 识别位点CGGWCCGGCCWGGCSacI 识别位点GAGCTCCTCGAGSacII 识别位点CCGCGGGGCGCCSalI 识别位点GTCGACCAGCTGSapI 识别位点GCTCTTCNCGAGAAGNSau3AI 识别位点GATCCTAGSau96I 识别位点GGNCCCCNGGSbfI 识别位点CCTGCAGGGGACGTCCAGTACTTCATGAScrFI 识别位点CCNGGGGNCCSexAI 识别位点ACCWGGTTGGWCCASfaNI 识别位点GCATCNCGTAGNSfcI 识别位点CTRYAGGAYRTCSfiI 识别位点GGCCNNNNNGGCCCCGGNNNNNCCGGSfoI 识别位点GGCGCCCCGCGGSgrAI 识别位点CRCCGGYGGYGGCCRCSmaI 识别位点CCCGGG GGGCCCSmlI识别位点CTYRAGGARYTCSnaBI识别位点TACGTAATGCATSpeI识别位点ACTAGTTGATCASphI识别位点GCATGCCGTACGSspI识别位点AATATTTTATAAStuI识别位点AGGCCTTCCGGAStyD4I识别位点CCNGGGGNCCStyI识别位点GCWWGGGGWWCCSwaI识别位点ATTTAAATTAAATTTATaqαI识别位点TCGAAGCTTfiI识别位点GAWTCCTWAGTliI识别位点CTCGAGGAGCTCTseI识别位点GCWGCCGWCGTsp45I识别位点GTSACCASTGTsp509I识别位点AATTTTAATspMI识别位点CCCGGGGGGCCCTspRI识别位点NNCASTGNNNNGTSACNNTth111I识别位点GACNNNGTC CTGNNNCAGXbaI识别位点TCTAGAAGATCTXcmI识别位点CCANNNNNNNNNTGG GGTNNNNNNNNNACCXhoI识别位点CTCGAGGAGCTCXmaI识别位点CCCGGGGGGCCCXmnI识别位点GAANNNNTTC CTTNNNNAAGZraI识别位点GACGTCCTGCAG与大家共享（非原创）。
限制性内切酶酶切位点汇总
ApaI识别位点Acc65I识别位点ApaLI识别位点AccI识别位点ApeKI识别位点AciI识别位点ApoI识别位点AclI识别位点AscI识别位点AcuI识别位点AseI识别位点AfeI识别位点AsiSI识别位点AflII识别位点AvaI识别位点AflIII识别位点AvaII识别位点AgeI识别位点AvrII识别位点AhdI识别位点BaeI识别位点AleI识别位点BamHI识别位点AluI识别位点BanI识别位点AlwI识别位点BanII识别位点AlwNI识别位点BmrI识别位点BbvCI识别位点BmtI识别位点BbvI识别位点BpmI识别位点BccI识别位点Bpu10I识别位点BceAI识别位点BpuEI识别位点BcgI识别位点BsaAI识别位点BciVI识别位点BsaBI识别位点BclI识别位点BsaHI识别位点BfaI识别位点BsaI识别位点BfuAI识别位点BsaJI识别位点BglI识别位点BsaWI识别位点BglII识别位点BsaXI识别位点BlpI识别位点BseRI识别位点Bme1580I识别位点BseYI识别位点BmgBI识别位点BspMI识别位点BsiEI识别位点BspQI识别位点BsiHKAI识别位点BsrBI识别位点BsiWI识别位点BsrDI识别位点BslI识别位点BsrFI识别位点BsmAI识别位点BsrGI识别位点BsmBI识别位点BsrI识别位点BsmFI识别位点BssHII识别位点BsmI识别位点BssKI识别位点BsoBI识别位点BssSI识别位点Bsp1286I识别位点BstAPI识别位点BspCNI识别位点BstBI识别位点BspDI识别位点BstEII识别位点BspEI识别位点BstNI识别位点BspHI识别位点DdeI识别位点BstXI识别位点DpnI识别位点BstYI识别位点BstZ17I识别位点DpnII识别位点Bsu36I识别位点DraI识别位点BtgI识别位点DraIII识别位点BtgZI识别位点DrdI识别位点BtsCI识别位点EaeI识别位点BtsI识别位点EaeI识别位点Cac8I识别位点EagI识别位点ClaI识别位点EarI识别位点CspCI识别位点EciI识别位点CviAII识别位点EcoNI识别位点CviKI-1识别位点EcoO109I识别位点CviQI识别位点EcoP15I识别位点EcoRI识别位点EcoRV识别位点FatI识别位点FauI识别位点Fnu4HI识别位点FokI识别位点FseI识别位点FspI识别位点HaeII识别位点HaeIIIHgaI识别位点HhaI识别位点HincII识别位点HindIII识别位点HinfI识别位点HinP1I识别位点HpaI识别位点HpaII识别位点HphI识别位点Hpy188I识别位点Hpy188III识别位点Hpy99I识别位点HpyAV识别位点HpyCH4III识别位点HpyCH4IV HpyCH4V识别位点KasI识别位点KpnI识别位点MboI识别位点MboII识别位点MfeI识别位点MluI识别位点MlyI识别位点MmeI识别位点MnlI识别位点MscI识别位点MseI识别位点MslI识别位点MspA1I识别位点MspI识别位点MwoI识别位点NaeI识别位点NarI识别位点NciI识别位点NdeI识别位点NgoMIV识别位点NheI识别位点NlaIII识别位点NlaIV识别位点NmeAIII识别位点NotI识别位点NruI识别位点NsiI识别位点NspI识别位点PacI识别位点PaeR7I识别位点PciI识别位点PflFI识别位点PflMI识别位点PhoI识别位点PleI识别位点PmeI识别位点PmlI识别位点PpuMI识别位点PshAI识别位点PsiI识别位点PspGI识别位点PspOMI识别位点PspXI识别位点PstI识别位点PvuI识别位点PvuII识别位点RsrII识别位点SacI识别位点SacII识别位点SalI识别位点SapI识别位点Sau3AI识别位点Sau96I识别位点SbfI识别位点ScaI识别位点ScrFI识别位点SexAI识别位点SfaNI识别位点SfcI识别位点SfiI识别位点SfoI识别位点SgrAI识别位点SmaI识别位点SmlI识别位点SnaBI识别位点SpeI识别位点SphI识别位点SspI识别位点StuI识别位点StyD4I识别位点StyI识别位点SwaI识别位点TaqαI识别位点TfiI识别位点TseI识别位点Tsp45I识别位点Tsp509I识别位点TspMI识别位点TspRI识别位点Tth111I识别位点XbaI识别位点XcmI识别位点XhoI识别位点XmaI识别位点XmnI识别位点ZraI识别位点。
史上最全限制性内切酶酶切位点汇总
HgaI识别位点 HhaI识别位点 HincII识别位点 HindIII识别位点 HinfI识别位点 HinP1I识别位点 HpaI识别位点 HpaII识别位点 HphI识别位点 Hpy188I识别位点 Hpy188III识别位点 Hpy99I识别位点 HpyAV识别位点 HpyCH4III识别位点 HpyCH4IV
HpyCH4V识别位点 KasI识别位点 KpnI识别位点 MboI识别位点 MboII识别位点 MfeI识别位点 MluI识别位点 MlyI识别位点 MmeI识别位点 MnlI识别位点 MscI识别位点 MseI识别位点 MslI识别位点 MspA1I识别位点
MspI识别位点 MwoI识别位点 NaeI识别位点 NarI识别位点 NciI识别位点 NcoI识别位点 NdeI识别位点 NgoMIV识别位点 NheI识别位点 NlaIII识别位点 NlaIV识别位点 NmeAIII识别位点
SbfI识别位点 ScaI识别位点 ScrFI识别位点 SexAI识别位点 SfaNI识别位点 SfcI识别位点 SfiI识别位点 SfoI识别位点 SgrAI识别位点 SmaI识别位点 SmlI识别位点 SnaBI识别位点 SpeI识别位点 SphI识别位点
SspI识别位点 StuI识别位点 StyD4I识别位点 StyI识别位点 SwaI识别位点 TaqαI识别位点 TfiI识别位点 TliI识别位点 TseI识别位点 Tsp45I识别位点 Tsp509I识别位点 TspMI识别位点
NotI识别位点 NruI识别位点
NsiI识别位点 NspI识别位点 PacI识别位点 PaeR7I识别位点 PciI识别位点 PflFI识别位点 PflMI识别位点 PhoI识别位点 PleI识别位点 PmeI识别位点 PmlI识别位点 PpuMI识别位点 PshAI识别位点 PsiI识别位点
限制性内切酶酶切位点汇总
Alel 识别位点Aatll 识别位点 5「・GACGTt ・・”3‘ G …CJGCAG …却Acc65l 识别位点5 _ GrtaKAC - 31 3：..CAKMJG..,5Accl 识别位点5. .-GfSnCAC .T.Sl3：..CAKMJG (5)Acil 识别位点S. - .C'feGC, ..3 3： ..GGCG. ,.5JAcll 识别位点$…从二 3“p ： .. TTGC/A .. .5'Acul 识别位点5： , XTG A AG(N)…\. . 3 p\. GACTTC<N}^ ・■ 5Afel 识别位点B -,AGC^JCT .. .3 忙…TCqp" . 5Aflll 识别位点5 ,.(TTI^AC,r.3-3：「G A A T 1T& (5)Afllll 识别位点吕…总RW.3UGgqA (5)Agel 识别位点&'、, Q GGGGT …E3： . . TGGCCi t A...5,Ahdl 识别位点6 . G ACNN L T NN GTC ,r 3 3： .XTGNb<NNNCAG. -515 . ..CACNrTNNGTG- ..33 ..GTGNN^hlNCAC . 5^Alul 识别位点5： .. AGtT ..*3 3.. . TCpA...5JAlwl 识别位点5 ..GGATC<N )7 . 3' 3： .. CC7 AG{N)，A . . 6HAlwNl 识别位点rT7CAGNNfftTG77.3" 3J ...GTq fc NNNGAC....5JApal 识别位点5：…GGGC<?t ,…h 3.. XpCGGG...5^ApaLl 识别位点$…密TGCAC …了 3」…CAGjG …5’ApeKl 识别位点5：…GSGC …3 扩…百GWGQ …护Apol 识别位点V — FTAAT T ¥ _ _3" 計…■节TTAAf …hAscl 识别位点5 …GGtGCGg …3" 3. . CCGC3CJ3G . 5JAsel 识别位点5 …A TT A AT …3" 扩…TA A7JAAsiSl 识别位点5 …GCGAffcGC.. 3 CGqr的CG .亍Aval 识别位点5 …CT¥CGRG,・3 3：…GHGCWC …5「Bcgi识别位点5. ..\/N)CGA{N>fl TGC(N)17，丁霞.十㈣GCT㈣』C®叭苫■ ■BciVI识别位点5；.. GTA TCC(Nj T33. .,CAT AGG(N}^. .5'Bcli识别位点5’…ft ATCA ■一3「3：.. ACTAGJ.…5JBamHI Bfai识别位点5：・.(Tr AG …丁3：”. G負咚；…亍BfuAI识别位点5：. ACCTGC(N>7 T3：…TGG ACG(N}g .hBgli识别位点5；. GCCMNhN^lGGC., ,3^3：. CGG^NhNNCCG 5Bglii识别位点5；. A^3 ATCT tt +3*3；.. TCTAi^A .. .5^Blpi识别位点5 …GgTNAGC …3"3：.. CGANT^CG .5"Bme1580l识别位点亍…GKGCWfc…;T扩…qMCGKG.. 5"BmgBI识别位点5：,.CAC?feTC.^3 扩…GTqfAG・5"Bmri识别位点5 ACTGGG(N)3T3"3. .. TGACCC(N)^ 5Bmti识别位点5；. ,GCTACrC,..3^3：. .CGATCG.-.5^Avail识别位点&…富...心CWGf (5)Avril识别位点比TAGG…旷P：..GGATCJD...5'Baei识别位点5； ^.^NlACfN^GTAYCfM)^ 一3”3 =JN)TG(NhCATRG(N)『..5“识别位点6.. .G^ATCC...3HOCTAQp…SBanl识别位点S：^G'bYRCC^,3-3.. .CCRY(\G...6*Ban II识别位点KRGCe …L y..C"GRG …亍Bbsi识别位点|5：.G A AG AC…:TB. …CT TCTG (Nk …弓-BbvCI识别位点H…CC T TCAGC…劭扩…右G由GLUG…亍Bbvl识别位点S P. .GCAGC<N)7 . ,33. . .CGT€G^N)l?i..5rBcci识别位点5 ATC(N)7 .3一卜-.GGTAG叫・hBceAI识别位点，…ACGGU(M)二3 3「.TGCCG(N)鉴「5”Bpml识别位点|5： .CTGG AG{N)V T.3^ p： ..G ACCTC(N)1Jk. .5 BpulOI识别位点5 …ccTrzGC …w GGANl^CG …引BpuEl识别位点p：C TTG AG(N)/. . r3*p. ..GAACTC(M)v^.t5-BsaAl识别位点t；YAC^GT A , 3P：.,RT(\CA? .5^BsaBI识别位点5： . .GATNN'klNATC . 3'p j. XTANN a NNTAG * hBsaHI识别位点亍…GhtGM…丁S^.CYGC^RG. -5JBsal识别位点B：. G G T C T C (肝,.3 p：. . CCAGAG |叽…hBsaJI识别位点5J. ..C'tNNGG.. 3p... GGNNC^. .5BsaWI识别位点b.「W\；UGG 內.・.3一WGGCC^W 6BsaXI识别位点p：\(N)AC (N)&CTCC («)f¥3’3 . rj(NHG(N)" GAGG(M). .BseRI识别位点5 .GAGG AG(N|/ T. . C TCCTC (N)q』…亍BseYI识别位点5 .X\：QAGC---3fl乳. £GGTqp…宙BsgI识别位点5. ..GTGC AG(N)16T.3^ 3 . .«C ACGTCtM),^ . ,5H BsiEI识别位点GGRA T G,3”3’…GCJFIGC；…5’BsiHKAI识别位点5：・.GWGCW七…古a^.^WCGWG ...5"BsiWI识别位点「庞TZG；…3"3’…右CA TG$ 5"BslI识别位点5J . CCNNNNlThJNGG . 3* 3r . GGNN^NNNNNCC …5" BsmAI识别位点5：GTCTC(NjJ 3"3. .. C AG AG (⑷脣…亍BsmBI识别位点5 ..CGTCTC (N)/ , 33 . ..GCAGAG (NK k.. 6' BsmFI识别位点5 …GGGAC^N)./. -3 驭…CCC1GR1-A..5 BsmI识别位点5. G AATQCM T3"3：CTTACGN 亍■BsoBI识别位点5： ..CYMRG ~3"3. ..GRGCY^C .5^Bsp1286l识别位点5】・「G D G C (3)3：…QHGGDG .…5’BspCNI识别位点and BssKI 识别位点BtgI 识别位点5： . ,CTC AG(N}J .33 . .G AGTC (N)s - .3 5…1tCMG …旷3.. GGNCq...5JBssSI 识别位点5 …疗2GAG •，丁 3JGTGG 即…亍BstAPI 识别位点5 .,GCAIMMN hfN TGC ...3 3.. .CGT\NNNNACG. (5)BstBI 识别位点5…TT 七GAA …3^ Y …AAGQTT …hBstEII 识别位点5r Tt l?feTNACC 3\,CGANTG j G r ..5*BstNI 识别位点5 …CtfwGG …丁 3. GG\\CC. 5BstUI 识别位点亍…CGtG, 33’…cqpc …5’BstXI 识别位点5., ,CCANNNNN T NTG G 77,33； GGTN^NNNNNACC £BstYI 识别位点5：「RST£¥ …3"YOTAQP …国BstZ17l 识别位点5. h f AC …3 3：.XAT ；ATG,..51Bsu36l 识别位点5 ...NAGG (3)3；..GGANT A OC.. 5BspDI 识别位点5；, . AffeG AT .3*.. . TAGCJA . ..5fBspEI 识别位点畀一代CGG 山■…丁3：.. AGGCCJ^.5^BspHI 识别位点|511T ftATGA.,3' P ：.. AGTACJ. . .51BspMI 识别位点6 . ACC7GC (N)J. .y r. .. TGGACG Bhj , SBspQI 识别位点fr ：..GCTOTTC(N^..T S\a .QQAGAAG^J .S ,BsrBI 识别位点5； ~CC^V T C,-3- 3J ...GGC^AG.-S^BsrDI 识别位点|5-.. GC A ATGNN^,.^* 卜…CGTTAqNN …5」BsrFI 识别位点AtCGG”…3" YGGCC^R ^5BsrGI 识别位点5 ：, UtTACA r -3" 3：.. AGATGJ. ,5JBsrI 识别位点S AC TGG N T. .3*3： . . TG ACf N …5，BssHII 识别位点5 VCGCGC., .3 J ,Dpnl 识别位点3： ..GGYRC^C ,5JBtgZI 识别位点i5P .GCGATG(N)/ 3* p ： ..CGCTAC(N),^ 5「BtsCI 识别位点fc ； CAG TG NN T,, 3 &\.CGTC ACNN..,5JBtsI 识别位点|5； ,T G CAG TG NN T,, J b, ,.CGTC AGNN r .,5JdkCac8l 识别位点GCN^GC.^3 扩…CGI^NCGClal 识别位点6；.. A T\3 GAT -.3^ 扩…TAGCJ A..SCspCI 识别位点5： 一 \n (N) CAA (N), G TGG(H)1；| 二3* ^.u^GTT^CACC^^^, 5^CviAII 识别位点fc^.C'XTG , . b 3* 卜…GTAf …hCviKI-1 识别位点5…R<TC¥ 一3 3"…¥GJ3Fl 一5CviQI 识别位点5：. <fTAC^,3' 孑...CATf (5)Ddel 识别位点5： — CfTfrJ AG.…：T 扩…昨冉蝌T 卫…玄CH, I ；5 G A T C ,33： CT AG 5"A lCH 3DpnII 识别位点5… $ AT 匚…丁 3；., CT AG a .. .5,DraI 识别位点煮「TT T% AR …了3\t AAAJTT ,.-5JDraIII 识别位点5 …CACNNN 力 TG …丁 勞…GTG&NM 忖CAG-眷DrdI 识别位点5 . ,.GAC MN MlAlNQTC, ,,3 3.. .CTGNN i NNNNCAG (5)EaeI 识别位点5 …ZbXCR …3“ 3.. .RCCGG^ . 5JEaeI 识别位点6；^/bGCCR...3J 3.. .RCCGG^.. 5JEagI 识别位点亍…西GGGG,…丁 扩…GCCGqp …5’EarI 识别位点5 ^CTCTTC (N) T …3 3. GAG A AG (N)^ .5EciI 识别位点5 \ GGCGGA^N^^ …3"3•…CCGCC"叽…5"EcoNI 识别位点5 …CCTN 枷 NNAGG …3"3 . . GG ANNNJM NTCC .,5' EcoO109I识别位点Hgal识别位点9：YCCNG^R.-S^ EcoP15l识别位点5 H CAGCAG(N)/ y 3\..GTCGTC(N)a^. (5)EcoRI识别位点5；, ,<?A AT TC ,3^ 扩…CTTA Af…亍EcoRV识别位点帀GAT^TOP^.CT AJ AG (5)Fatl识别位点5：・N A TG (3)3 .. GTAC iL..5JFaul识别位点IS CCCGC ... 3* A「GGGCG(N)蛀…亍Fnu4HI识别位点F …GbNGC：…3" 护…CG M&G…5"Fokl识别位点.. G G A TG(N)/ 一3 p J. . . CCT AC(N),^ , .5' Fsel识别位点■….GGCCGCTbC；…•才a：.. CGpGCCGG . 5^ Fspl识别位点TG氏C—聞3：.. AC<^pGT...,5Haell识别位点5 …RGCGC TV…：r 扩…YpGGGR-仔5 .G ACGC (忖3： .,CTGCG^N}^ . 5r Hhal识别位点5 …GCGt：, ..3P 3：..CpCG..k5^Hin cll识别位点5…GT 沖2・33：…C為堂TG —hHin dlll 识别位点5…/C A GCTT・・・3「孑…T …hHi nfl识别位点5…G^NTC …b3’..."N比G (5)HinPII识别位点5, ..Gt GO ・・3 3：…CGCQ …hHpal识别位点亍…G* Tk AC…3’3.. .CAAJTG...5^Hpall识别位点5 ….C^GG , . .3' 扩…GgG…中Hphl识别位点5 GGTGA (呵「…3’31…X ACT叽…hHpy188l识别位点新...T C『G A (3)3 .., AGjlCT (5)Hpy188lll识别位点5.. r7CTNNGA...3H 計…典GNNfT-hHpy99l识别位点5r. r r CGWCG T -3'S-.^GCWGC. ,.5JHaelllMnII 识别位点HpyAV 识别位点S ：.,CCTTC(N)/,.,3, 3\ GGA AG{N)^ . 5^HpyCH4III 识别位点|5*.,. ACN^ST ...M 卜...T(^N C A (5)HpyCH4IV HpyCH4V 识别位点出…3“人砂丁…hKasl 识别位点亍…翻CGCC., 丁 缶…CCGC<\p …hKpnl 识别位点6. ..GGT AC^T. ,3' 吿…qpATdG …刖Mbol 识别位点乔0ATC …3” 卜…GTg ,.5"Mboll 识别位点|5■…GAAGA (N)J ,3 忙…CTTCT (N)“ _SMfel 识别位点C ATTG …丁 3…GYTAAJ3…柠Mlul 识别位点y.fCGCGT 3 3J ^TGCG^A., 51Mlyl 识别位点5 . G AGTC{NJ7 3' 3 . GTCAG (NL ・.EMmel 识别位点h …TCC RAC (州广…3" p ； . . AGGYTG(N)tir ..5J 5r . CCTC (N)J 3 扩…GGAG ㈣;Mscl 识别位点 5 TGC^CCA.,,3*3：.. ACCpGT...5JMsel 识别位点5"…TP AA …丁 3. h . A AT^T 5FMsll 识别位点S ;.1CAYNN 1F MNRTG ^.3^3r .. CTRhll^NN YAC hMspAll 识别位点5 .. T CM<St KG ,…3’ 茁…GKqp 衲C..6Mspl 识别位点5.. .C?bGG.,,3^ 扩…GGqC ….5’Mwol 识别位点5.. .GCNNNNN*NNGC ・_3 3r . CGNN^NNNNNCG .5"Nael 识别位点5…GC 祠GC …3’ 3：..CGG b CCG...5-Narl 识别位点亍,G 比GGC …3^ccotyse (x)Ncil 识别位点亍…CC*SGG …;r3 .. .GGS^CC. .5*Ncol 识别位点亍…牝ATGG …聞 扩…GGTACQ …SNdel 识别位点C/fT ATG …了 3：..GT AT ；AC …5’NgoMlV 识别位点6：..<?tCGGC ••卫ET GGG右於…5」Nhel识别位点6："Gt TAGC...3" 缶…CGATQG…hNlalll 识别位点S. ..CATC T ..aNlalV识别位点$p GGEg…y3：.. GCh|b NGG...5JNmeAlll识别位点5^GCCGAG(N)SM*-.3,gGQTC(N)s ・ FNotl识别位点吕….GCtGCCGC,「歹工…©右CCGGjf右…亍Nrul识别位点y…TCCftG島…丁3.. . AGCpGT .月Nsil识别位点杖…ATGG肝「丁3.. . V CQT* -5JNspl识别位点$…RCAT(?¥…旷p\ ..^GTACR . .5^Pacl识别位点砧…TTAA F T片山『3 ... A A T J A A T T (5)PaeR7l识别位点5\r^TCGAG r3'3\ GAGCT b C (5)Pcil识别位点$…心AT GT ■二① 卜…TG"qA.. E PfIFI识别位点^-.GAGhTNNQTC ...3*a^.CTGNN^NCAG (5)PfIMl识别位点5；ANNN^NTGG ・・，3 3 ^GGTN A NNhlNACC..,5Phol识别位点5：・・GGtC…孑Y…CCQD…弓Plel识别位点6 . . GAGTC (NjJ 33「…GTCAG ㈣.…5’Pmel识别位点5 …GTTT% 応AG …3“3…GA A AJT TG…弓Pmll识别位点S .-.CAC'GTC .•同3J ..GTG^AC …亍PpuMl识别位点Y...¥CC WQGB (6)PshAl识别位点5；..GACNN T NNGTC, ..a* 3 …CTGNN』NC2.-£Psil识别位点5 二・TTfT八WATT.•同PspGl识别位点5：. ECWGG -糾3’…GGWgSPspOMI识别位点&,.,<fGGCCC7.,3J.CCGGqp-. +5'PspXl识别位点Sbfl 识别位点3^ .GG/CGTCC 5^Seal 识别位点5 .. T AGrtCT , ir 3' 3.. . TCAJGA ...5^ScrFl 识别位点5…“NGG …3 3：..GGy SSexAl 识别位点5,—XtCWGGT?.^' 3：..TGGWCqA (5)SfaNl 识别位点5 .. GCATC (N)J. .3-GGTAG (N)^...5_Sfcl 识别位点5 …(7TR¥AG …3" 3r .. G AYR*QC .. .5"Sfil 识别位点5 …GGCCMN N EGGCC …3" 3 .CCGGI^NNNNCCGG 5JSfol 识别位点WGG 显CC …3” 3「CgCGG …5」SgrAl 识别位点亍…Crft GGGg.33' .GYGGCC^RC . 5*Smal 识别位点TCC^&GG ?3 ..GGGpCC.. 5'Smll 识别位点亍…brifRAG …暫3'. . ,G A R Y T ；C, ..5FSnaBl 识别位点5二・TAC^GTA ・・・3 3J . ..A TGC AT... S'&…VtfTCGAGB3F .BGAGCT^CV r 5^Pstl 识别位点F …CTGC R …：rIT …qkUGTC . 5’Pvul 识别位点・.一 CGATtG.「孑3.. -GCJ AG J .5*Pvull 识别位点15, , CA<?tTG. ,.3 P ： . GTCpAC.,. 5^Rsal 识别位点F ・・・GTN C ,「才3\.CAJG..5JRsrll 识别位点b …GGCWCipC …中Sacl 识别位点S ： ..G AGCT'fc., .3 扩…C.TCG 占G.. SSacll 识别位点S ；,.CCGtfbG.,.3 3 . ..GGJ3GCC . , .5JSall 识别位点$…G T TCGAC …:rp J ...CAGCT (i G (5)Sapl 识别位点$…GCTUT TC 慣卓3(3. ,,CGAGAAG(N)Jk .,5^Sau3AI 识别位点5 .. 7c A T c - 3^ S- .-CTAG^ . .5*Sau96l 识别位点S^-GlSNCC-. ,3rSpel识别位点6；../fCTAGT, r.3'TGATG/ .-S^ Sphl识别位点6；.. G C A T dt...3^CjST ACG …5 5.. ^TS AC.. .3r Tsp509l识别位点5’…1r T …丁雷…T T A A^. . 5H TspMl识别位点5\. C*CCGGG 3…GGGCC&Sspl识别位点6：,. AA T工TT …N 缶…TT AJAA . ..5J Stul识别位点TspRl识别位点5…村MCASTGN3 3.,^MGTSACNN-..5^ Tthllll识别位点StyD4l识别位点5..,GACN T NNGTC rrT3" S.-XTONN^NCAG. ..5'$：”乂CNGG (3)3：..GGNCq..6Styl识别位点WCWWGG …3,3 +. .6 G WWC^C 一5「Swal识别位点5 ：.. A T T A A T ,.. 3 3：.. T A AAJ TT A.. 5 Taq aI识别位点S…让GA…$3：.. AGCJ (5)Tfil识别位点5： ,,G^W rc ,,3 3...CTWAJ3...5"Tlil识别位点6：T.C T CGAG-7,3*3R .GAGCTJ3 . 5^Tsel识别位点•・・G'bWG£・*3"a p ^CGWCjG …5 Xbal识别位点5 …ffcT AGA …3“3... AGA TC^T . ..5'Xcml识别位点5, ANNNNN'NNNNTGG...3,3r GGT NNNh^NN NN NACC. . 5^ Xhol识别位点5“…疗TCGAG…可31…G負GC“ …亍Xmal识别位点煮…比QGGG…;T 勞…GGGCqC…5’Xmnl识别位点5.1+GAANN^MTTC .…3"3：. XT TN N^N MA AG .. 5"Zral识别位点h…G現龙TC…①3：. CTGjGAG--. 5'Tsp45l识别位点。
史上最全限制性内切酶酶切位点汇总情况
A系列AatII识别位点Acc65I识别位点AccI识别位点AciI识别位点AclI识别位点AcuI识别位点AfeI识别位点AflII识别位点AflIII识别位点AgeI识别位点AhdI识别位点AleI识别位点AluI识别位点AlwI识别位点AlwNI识别位点ApaI识别位点ApaLI识别位点ApeKI识别位点ApoI识别位点AscI识别位点AseI识别位点AsiSI识别位点AvaI识别位点AvaII识别位点AvrII识别位点BaeI识别位点BamHI 识别位点BanI识别位点BanII识别位点BbvCI识别位点BbvI识别位点BccI识别位点BceAI识别位点BcgI识别位点BciVI识别位点BclI识别位点BfaI识别位点BfuAI识别位点BglI识别位点BglII识别位点BlpI识别位点Bme1580I识别位点BmgBI识别位点BmrI识别位点BmtI识别位点BpmI识别位点Bpu10I识别位点BpuEI识别位点BsaAI识别位点BsaBI识别位点BsaHI识别位点BsaI识别位点BsaJI识别位点BsaWI识别位点BsaXI识别位点BseRI识别位点BseYI识别位点BsiEI识别位点BsiHKAI识别位点BsiWI识别位点BslI识别位点BsmAI识别位点BsmBI识别位点BsmFI识别位点BsmI识别位点BsoBI识别位点Bsp1286I识别位点BspCNI识别位点BspDI识别位点BspEI识别位点BspHI识别位点BspMI识别位点BspQI识别位点BsrBI识别位点BsrDI识别位点BsrFI识别位点BsrGI识别位点BsrI识别位点BssHII识别位点BssKI识别位点BssSI识别位点BstAPI识别位点BstBI识别位点BstEII识别位点BstNI识别位点BstXI识别位点BstYI识别位点BstZ17I识别位点Bsu36I识别位点BtgI识别位点BtgZI识别位点BtsCI识别位点BtsI识别位点Cac8I识别位点ClaI识别位点CspCI识别位点CviAII识别位点CviKI-1识别位点CviQI识别位点DdeI识别位点DpnI识别位点DpnII识别位点DraI识别位点DraIII识别位点DrdI识别位点EaeI识别位点EaeI识别位点EagI识别位点EarI识别位点EciI识别位点EcoNI识别位点EcoO109I 识别位点EcoRI识别位点EcoRV识别位点FatI识别位点FauI识别位点Fnu4HI识别位点FokI识别位点FseI识别位点FspI识别位点HaeII识别位点HaeIIIHgaI识别位点HhaI识别位点HincII识别位点HindIII识别位点HinfI识别位点HinP1I识别位点HpaI识别位点HpaII识别位点HphI识别位点Hpy188I识别位点Hpy188III识别位点Hpy99I识别位点HpyAV识别位点HpyCH4III识别位点HpyCH4IV HpyCH4V识别位点KasI识别位点KpnI识别位点MboI识别位点MboII识别位点MfeI识别位点MluI识别位点MlyI识别位点MmeI识别位点MnlI识别位点MscI识别位点MseI识别位点MslI识别位点MspA1I识别位点MspI识别位点MwoI识别位点NaeI识别位点NarI识别位点NciI识别位点NdeI识别位点NgoMIV识别位点NheI识别位点NlaIII识别位点NlaIV识别位点NmeAIII识别位点NotI识别位点NruI识别位点NsiI识别位点NspI识别位点PacI识别位点PaeR7I识别位点PciI识别位点PflFI识别位点PflMI识别位点PhoI识别位点PleI识别位点PmeI识别位点PmlI识别位点PpuMI识别位点PshAI识别位点PsiI识别位点PspGI识别位点PspOMI识别位点PspXI识别位点PstI识别位点PvuI识别位点PvuII识别位点RsrII识别位点SacI识别位点SacII识别位点SalI识别位点SapI识别位点Sau3AI识别位点Sau96I识别位点SbfI识别位点ScaI识别位点ScrFI识别位点SexAI识别位点SfaNI识别位点SfcI识别位点SfiI识别位点SfoI识别位点SgrAI识别位点SmaI识别位点SmlI识别位点SnaBI识别位点SpeI识别位点SphI识别位点SspI识别位点StuI识别位点StyD4I识别位点StyI识别位点SwaI识别位点TaqαI识别位点TfiI识别位点TseI识别位点Tsp45I识别位点Tsp509I识别位点TspMI识别位点TspRI识别位点Tth111I识别位点XbaI识别位点XcmI识别位点XhoI识别位点XmaI识别位点XmnI识别位点ZraI识别位点。
限制性内切酶酶切位点_方便搜索(word版本)
常用NOT1和Sbf1识别位点(正向引物前加NOT1;反向引物前加sbf1.)AatII 识别位点GACGTCCTGCAGAcc65I 识别位点GTMKACCAKMTGAccI 识别位点GTMKACCAKMTGAciI 识别位点CCGCGGCGAclI 识别位点AACGTTTTGCAAAcuI 识别位点CTGAAGGACTTCAfeI 识别位点AGCGCTTCGCGAAflII 识别位点CTTAAGGAATTCAflIII 识别位点ACRYGTTGYRCAAgeI 识别位点ACCGGTTGGCCAAhdI 识别位点GACNNNNNGTCCTGNNNNNCAGAleI 识别位点CACNNNNGTGGTGNNNNCACAluI 识别位点AGCTTCGAAlwI 识别位点GGATCCCTAGAlwNI 识别位点CAGNNNCTGGTCNNNGACApaI 识别位点GGGCCCCCCGGGGTGCACCACGTGApeKI 识别位点GCWGCCGWCGApoI 识别位点RAATTYYTTAARAscI 识别位点GGCGCGCCCCGCGCGGAseI 识别位点ATTAATTAATTAAsiSI 识别位点GCGATCGCCGCTAGCGAvaI 识别位点CYCGRGGRGCYCAvaII 识别位点GGWCCCCWGGCCTAGGGGATCCBaeI 识别位点NACNGTAYCNBamHI 识别位点GGATCCCCTAGGBanI 识别位点GGYRCCCCRYGGBanII 识别位点GRGCYCCYCGRGBbsI 识别位点GAAGACCTTCTGBbvCI 识别位点CCTCAGCGGAGTCGBbvI 识别位点GCAGCCGTCGCCATCGGTAGBceAI 识别位点ACGGC TGCCGBcgI 识别位点CGANTGNGCTNACGBciVI 识别位点GTATCCCATAGGBclI 识别位点TGATCAACTACTBfaI 识别位点CTAGGATCBfuAI 识别位点ACCTGCTGGACGBglI 识别位点GCCNNNNNGGCCGGNNNNNCCGAGATCTTCTAGABlpI 识别位点GCTNAGCCGANTCGBme1580I 识别位点GKGCMCCMCGKGBmgBI 识别位点CACGTCGTGCAGBmrI 识别位点ACTGGGNTGACCCNBmtI 识别位点GCTAGCCGATCGBpmI 识别位点CTGGAGNGACCTCNBpu10I 识别位点CCTNAGCGGANTCGCTTGAGNGAACTCNBsaAI 识别位点YACGTRRTGCAYBsaBI 识别位点GATNNNNATCCTANNNNTAGBsaHI 识别位点GRCGYCCYGCRGBsaI 识别位点GGTCTCNCCAGAGNBsaJI 识别位点CCNNGGGGNNCCBsaWI 识别位点WCCGGWWGGCCWBsaXI 识别位点NACNCTCCNNTGNGAGGNGAGGAGNCTCCTCNBseYI 识别位点CCCAGCGGGTCGBsgI 识别位点GTGCAGCACGTCBsiEI 识别位点CGRYCGGCYRGCBsiHKAI 识别位点GWGCWCCWCGWGBsiWI 识别位点CGTACGGCATGCBslI 识别位点CCNNNNNNNGGGGNNNNNNNCCBsmAI 识别位点GTCTCNCAGAGNCGTCTCNGCAGAGNBsmFI 识别位点GGGACCCCTGBsmI 识别位点GAATGCNCTTACGNBsoBI 识别位点CYCGRGGRGCYCBsp1286I 识别位点GDGCHCCHCGDGBspCNI 识别位点CTCAGN GAGTCNBspDI 识别位点ATCGATTAGCTABspEI 识别位点TCCGGAAGGCCTTCATGAAGTACTBspMI 识别位点ACCTGCNTGGACGNBspQI 识别位点GCTCTTCNCGAGAAGNBsrBI 识别位点CCGCTCGGCGAGBsrDI 识别位点GCAATGNNCGTTACNNBsrFI 识别位点RCCGGYYGGCCRBsrGI 识别位点TGTACAACATGTBsrI 识别位点ACTGGNTGACCNGCGCGCCGCGCGBssKI 识别位点CCNGGGGNCCBssSI 识别位点CACGAGGTGCTCBstAPI 识别位点GCANNNNNTGCCGTNNNNNACGBstBI 识别位点TTCGAAAAGCTTBstEII 识别位点GGTNACCCCANTGGBstNI 识别位点CCWGGGGWCCBstUI 识别位点CGCGGCGCCCANNNNNNTGGGGTNNNNNNACCBstYI 识别位点RGATCYYCTAGRBstZ17I 识别位点GTATACCATATGBsu36I 识别位点CCTNAGGGGANTCCBtgI 识别位点CCRYGGGGYRCCBtgZI 识别位点GCGATGCGCTACBtsCI 识别位点GCAGTGNNCGTCACNNBtsI 识别位点GCAGTGNNCGTCACNNGCNNGCCGNNCGClaI 识别位点ATCGATTAGCTACspCI 识别位点NCAANGTGGNNGTTNCACCNCviAII 识别位点CATGGTACCviKI-1识别位点RGCYYCGRCviQI 识别位点GTACCATGDdeI 识别位点CTNAGGANTCDpnI 识别位点GATCCTAGGATCCTAGDraI 识别位点TTTAAAAAATTTDraIII 识别位点CACNNNGTGGTGNNNCACDrdI 识别位点GACNNNNNNGTCCTGNNNNNNCAGEaeI 识别位点YGGCCRRCCGGYEagI 识别位点CGGCCGGCCGGCEarI 识别位点CTCTTCGAGAAGEciI 识别位点GGCGGACCGCCTCCTNNNNNAGGGGANNNNNTCCEcoO109I 识别位点RGGNCCYYCCNGGREcoP15I 识别位点CAGCAGGTCGTCEcoRI 识别位点GAATTCCTTAAGEcoRV 识别位点GATATCCTATAGFatI 识别位点CATGGTACFauI 识别位点CCCGCGGGCGFnu4HI 识别位点GCNGCCGBCGGGATGNCCTACNFseI 识别位点GGCCGGCCCCGGCCGGFspI 识别位点TGCGCAACGCGTHaeII 识别位点RGCGCYYCGCGRHaeIIIHgaI 识别位点GACGCCTGCGHhaI 识别位点GCGCCGCGHincII 识别位点GTYRACCARYTGHindIII 识别位点AAGCTTTTCGAAGANTCCTNAGHinP1I 识别位点GCGCCGCGHpaI 识别位点GTTAACCAATTGHpaII 识别位点CCGGGGCCHphI 识别位点GGTGACCACTHpy188I 识别位点TCNGAAGNCTHpy188III 识别位点TCNNGAAGNNCTHpy99I 识别位点CGWCGGCWGCCCTTCGGAAGHpyCH4III 识别位点ACNGTTGCCAHpyCH4IVHpyCH4V 识别位点TGCAACGTKasI 识别位点GGCGCCCCGCGGKpnI 识别位点GGTACCCCATGGMboI 识别位点GATCCTAGMboII 识别位点GAAGACTTCTMfeI 识别位点CAATTGGTTAACACGCGTTGCGCAMlyI 识别位点GAGTCCTCAGMmeI 识别位点TCCRACAGGYTGMnlI 识别位点CCTCGGAGMscI 识别位点TGGCCAACCGGTMseI 识别位点TTAAAATTMslI 识别位点CAYNNNNRTGGTRNNNNYACMspA1I 识别位点CMGCKGGKCGMCCCGGGGCCMwoI 识别位点GCNNNNNNNGCCGNNNNNNNCGNaeI 识别位点GCCGCCGGCCGNarI 识别位点GGCGCCCCGCGGNciI 识别位点CCSGGGGSCCNcoI 识别位点CCATGGGGTACCNdeI 识别位点CATATG GTATACNgoMIV识别位点GCCGGC CGGCCGGCTAGCCGATCGNlaIII 识别位点CATGGTACNlaIV 识别位点GGNNCCCCNNGGNmeAIII 识别位点GCCGAGCGGCTCNotI 识别位点GCGGCCGC CGCCGGCGNruI 识别位点TCGCGAAGCGCTNsiI 识别位点ATGCAT TACGTANspI识别位点RCATGY YGTACRTTAATTAA AATTAATTPaeR7I识别位点CTCGAG GAGCTCPciI 识别位点ACATGT TGTACAPflFI识别位点GACNNNGTC CTGNNNCAGPflMI 识别位点CCANNNNNTGGGGTNNNNNACCPhoI 识别位点GGCCPleI识别位点GAGTCN CTCAGNPmeI 识别位点GTTTAAAC CAAATTTGPmlI识别位点CACGTG GTGCACRGGWCCYYCCWGGRPshAI 识别位点GACNNNNGTCCTGNNNNCAGPsiI 识别位点TTATAAAATATTPspGI 识别位点CCWGGGGWCCPspOMI 识别位点GGGCCCCCCGGGPspXI 识别位点VCTCGAGBBGAGCTCVPstI 识别位点CTGCAGGACGTCPvuI 识别位点CGATCGGCTAGCCAGCTGGTCGACRsaI 识别位点GTACCATGRsrII 识别位点CGGWCCGGCCWGGCSacI 识别位点GAGCTCCTCGAGSacII 识别位点CCGCGGGGCGCCSalI 识别位点GTCGACCAGCTGSapI 识别位点GCTCTTCNCGAGAAGNSau3AI 识别位点GATCCTAGGGNCCCCNGGSbfI 识别位点CCTGCAGGGGACGTCCScaI 识别位点AGTACTTCATGAScrFI 识别位点CCNGGGGNCCSexAI 识别位点ACCWGGTTGGWCCASfaNI 识别位点GCATCNCGTAGNSfcI 识别位点CTRYAGGAYRTCSfiI 识别位点GGCCNNNNNGGCCCCGGNNNNNCCGGGGCGCCCCGCGGSgrAI 识别位点CRCCGGYGGYGGCCRCSmaI 识别位点CCCGGGGGGCCCSmlI 识别位点CTYRAGGARYTCSnaBI 识别位点TACGTAATGCATSpeI 识别位点ACTAGTTGATCASphI 识别位点GCATGCCGTACGSspI 识别位点AATATTTTATAAStuI 识别位点AGGCCTTCCGGAStyD4I 识别位点CCNGGGGNCCStyI 识别位点GCWWGGGGWWCCSwaI 识别位点ATTTAAATTAAATTTATaqαI 识别位点TCGAAGCTTfiI 识别位点GAWTCCTWAGTliI 识别位点CTCGAGGAGCTCTseI 识别位点GCWGCCGWCGTsp45I 识别位点GTSACCASTGTsp509I 识别位点AATTTTAATspMI 识别位点CCCGGGGGGCCCTspRI 识别位点NNCASTGNNNNGTSACNNTth111I 识别位点GACNNNGTCCTGNNNCAGXbaI 识别位点TCTAGAAGATCTXcmI 识别位点CCANNNNNNNNNTGGGGTNNNNNNNNNACCXhoI 识别位点CTCGAGGAGCTCXmaI识别位点CCCGGG GGGCCCXmnI识别位点GAANNNNTTC CTTNNNNAAGZraI识别位点GACGTC CTGCAG与大家共享（非原创）。
限制性内切酶酶切位点汇总
RsaI 识别位点 RsrII 识别位点 SacI 识别位点 SacII 识别位点 SalI 识别位点 SapI 识别位点 Sau3AI 识别位点 Sau96I 识别位点 SbfI 识别位点 ScaI 识别位点 ScrFI 识别位点 SexAI 识别位点 SfaNI 识别位点 SfcI 识别位点 SfiI 识别位点
AatII 识别位点 Acc65I 识别位点 AccI 识别位点 AciI 识别位点 AclI 识别位点 AcuI 识别位点 AfeI 识别位点 AflII 识别位点 AflIII 识别位点 AgeI 识别位点 AhdI 识别位点 AleI 识别位点 AluI 识别位点 AlwI 识别位点 AlwNI 识别位点
BstUI 识别位点 BstXI 识别位点 BstYI 识别位点 BstZ17I 识别位点 Bsu36I 识别位点 BtgI 识别位点 BtgZI 识别位点 BtsCI 识别位点 BtsI 识别位点 Cac8I 识别位点
ClaI 识别位点 CspCI 识别位点 CviAII 识别位点 CviKI-1 识别位点 CviQI 识别位点
ApaI 识别位点 ApaLI 识别位点 ApeKI 识别位点 ApoI 识别位点 AscI 识别位点 AseI 识别位点 AsiSI 识别位点 AvaI 识别位点 AvaII 识别位点 AvrII 识别位点 BaeI 识别位点 BamHI 识别位点 BanI 识别位点 BanII 识别位点
BbsI 识别位点 BbvCI 识别位点 BbvI 识别位点 BccI 识别位点 BceAI 识别位点 BcgI 识别位点 BciVI 识别位点 BclI 识别位点 BfaI 识别位点 BfuAI 识别位点 BglI 识别位点 BglII 识别位点 BlpI 识别位点 Bme1580I 识别位点 BmgBI 识别位点
常用限制性内切酶酶切位点汇总
常用限制性内切酶酶切位点汇总Acc65I识别位点AccI识别位点AciI识别位点AclI识别位点AcuI识别位点AfeI识别位点AflII识别位点AflIII识别位点AgeI识别位点AhdI识别位点AleI识别位点AluI识别位点AlwI识别位点AlwNI识别位点ApaI识别位点ApaLI识别位点ApeKI识别位点ApoI识别位点AscI识别位点AseI识别位点AsiSI识别位点AvaI识别位点AvaII识别位点AvrII识别位点BaeI识别位点BamHI识别位点BanI识别位点BbvCI识别位点BbvI识别位点BccI识别位点BceAI识别位点BcgI识别位点BciVI识别位点BclI识别位点BfaI识别位点BfuAI识别位点BglI识别位点BglII识别位点BlpI识别位点Bme1580I识别位点BmgBI识别位点BmrI识别位点BmtI识别位点BpmI识别位点Bpu10I识别位点BpuEI识别位点BsaAI识别位点BsaBI识别位点BsaHI识别位点BsaI识别位点BsaJI识别位点BsaWI识别位点BsaXI识别位点BseRI识别位点BseYI识别位点BsiHKAI识别位点BsiWI识别位点BslI识别位点BsmAI识别位点BsmBI识别位点BsmFI识别位点BsmI识别位点BsoBI识别位点Bsp1286I识别位点BspCNI识别位点BspDI识别位点BspEI识别位点BspHI识别位点BspMI识别位点BspQI识别位点BsrBI识别位点BsrDI识别位点BsrFI识别位点BsrGI识别位点BsrI识别位点BssHII识别位点BssKI识别位点BssSI识别位点BstAPI识别位点BstBI识别位点BstEII识别位点BstNI识别位点BstXI识别位点BstYI识别位点BstZ17I识别位点BtgI识别位点BtgZI识别位点BtsCI识别位点BtsI识别位点Cac8I识别位点ClaI识别位点CspCI识别位点CviAII识别位点CviKI-1识别位点CviQI识别位点DdeI识别位点DpnI识别位点DpnII识别位点DraI识别位点DraIII识别位点DrdI识别位点EaeI识别位点EaeI识别位点EagI识别位点EarI识别位点EciI识别位点EcoNI识别位点EcoO109I识别位点EcoP15I识别位点EcoRI识别位点EcoRV识别位点FatI识别位点FauI识别位点Fnu4HI识别位点FseI识别位点FspI识别位点HaeII识别位点HaeIIIHgaI识别位点HhaI识别位点HincII识别位点HindIII识别位点HinfI识别位点HinP1I识别位点HpaI识别位点HpaII识别位点HphI识别位点Hpy188I识别位点Hpy188III识别位点Hpy99I识别位点HpyAV识别位点HpyCH4III识别位点HpyCH4IV HpyCH4V识别位点KasI识别位点KpnI识别位点MboI识别位点MboII识别位点MfeI识别位点MluI识别位点MlyI识别位点MmeI识别位点MnlI识别位点MscI识别位点MslI识别位点MspA1I识别位点MspI识别位点MwoI识别位点NaeI识别位点NarI识别位点NciI识别位点NdeI识别位点NgoMIV识别位点NheI识别位点NlaIII识别位点NlaIV识别位点NmeAIII识别位点NotI识别位点NruI识别位点NsiI识别位点NspI识别位点PacI识别位点PaeR7I识别位点PciI识别位点PflFI识别位点PflMI识别位点PhoI识别位点PleI识别位点PmeI识别位点PmlI识别位点PpuMI识别位点PshAI识别位点PsiI识别位点PspOMI识别位点PspXI识别位点PstI识别位点PvuI识别位点PvuII识别位点RsrII识别位点SacI识别位点SacII识别位点SalI识别位点SapI识别位点Sau3AI识别位点Sau96I识别位点SbfI识别位点ScaI识别位点ScrFI识别位点SexAI识别位点SfaNI识别位点SfcI识别位点SfiI识别位点SfoI识别位点SgrAI识别位点SmaI识别位点SmlI识别位点SnaBI识别位点SpeI识别位点SphI识别位点SspI识别位点StuI识别位点StyD4I识别位点SwaI识别位点TaqαI识别位点TfiI识别位点TseI识别位点Tsp45I识别位点Tsp509I识别位点TspMI识别位点TspRI识别位点Tth111I识别位点XbaI识别位点XcmI识别位点XhoI识别位点XmaI识别位点XmnI识别位点ZraI识别位点。
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常见分子实验限制性内切酶酶切位点大全
公司内部编号：（GOOD-TMMT-MMUT-UUPTY-UUYY-DTTI-
AatII 识别位点
Acc65I 识别位点
AccI 识别位点
AciI 识别位点
AclI 识别位点
AcuI 识别位点
AfeI 识别位点
AflII 识别位点
AflIII 识别位点
AgeI 识别位点
AhdI 识别位点
AleI 识别位点
AluI 识别位点
AlwI 识别位点
AlwNI 识别位点
ApaI 识别位点
ApaLI 识别位点
ApeKI 识别位点
ApoI 识别位点
AscI 识别位点
AseI 识别位点
AsiSI 识别位点
AvaI
识别位点
AvaII
识别位点
AvrII
识别位点
BaeI 识别位点
BamHI
识别位点
BanI
识别位点
BanII
识别位点
BbsI
识别位点
BbvCI
识别位点
BbvI
识别位点
BccI识别位点BceAI
识别位点
BcgI识别位点
BciVI识别位点BclI 识别位点
BfaI识别位点
BfuAI识别位点
BglI识别位点
BglII识别位点
BlpI识别位点
Bme1580I识别位点BmgBI识别位点
BmrI
识别位点
BmtI
识别位点
BpmI 识别位点
Bpu10I
识别位点
BpuEI
识别位点
BsaAI
识别位点
BsaBI
识别位点
BsaHI
识别位点
BsaI
识别位点
BsaJI
识别位点
BsaWI 识别位点
BsaXI 识别位点
BseRI 识别位点
BseYI 识别位点
BsgI 识别位点
BsiEI 识别位点
BsiHKAI 识别位点
BsiWI 识别位点
BslI
识别位点
BsmAI
识别位点
BsmBI 识别位点
BsmFI 识别位点
BsmI识别位点
BsoBI识别位点Bsp1286I
识别位点
BspCNI识别位点
BspDI识别位点BspEI
识别位点
BspHI识别位点
BspMI识别位点
BspQI识别位点
BsrBI识别位点
BsrDI识别位点
BsrFI识别位点
BsrGI
识别位点
BsrI
识别位点
BssHII识别位点
BssKI
识别位点
BssSI识别位点
BstAPI识别位点
BstBI识别位点
BstEII识别位点
BstNI识别位点
BstUI识别位点
BstXI
识别位点
BstYI
识别位点
BstZ17I
识别位点
Bsu36I
识别位点
BtgI 识别位点
BtgZI 识别位点
BtsCI 识别位点
BtsI
识别位点
Cac8I 识别位点
ClaI
识别位点
CspCI 识别位点
CviAII 识别位点
CviKI-1
识别位点
CviQI 识别位点
DdeI 识别位点
DpnI 识别位点
DpnII 识别位点
DraI
识别位点
DraIII 识别位点
DrdI 识别位点
EaeI 识别位点
EaeI
识别位点
EagI
识别位点
EarI
识别位点
EciI
识别位点
EcoNI 识别位点
EcoO109I
识别位点
EcoP15I 识别位点
EcoRI 识别位点
EcoRV 识别位点
FatI 识别位点
FauI 识别位点
Fnu4HI 识别位点
FokI 识别位点
FseI 识别位点
FspI 识别位点
HaeII 识别位点
HaeIII
HgaI 识别位点
HhaI 识别位点
HincII 识别位点
HindIII 识别位点
HinfI 识别位点
HinP1I识别位点HpaI识别位点HpaII识别位点HphI识别位点
Hpy188I识别位点Hpy188III识别位点Hpy99I识别位点HpyAV识别位点HpyCH4III识别位点
HpyCH4IV
HpyCH4V识别位点
KasI识别位点
KpnI识别位点
MboI识别位点
MboII识别位点
MfeI识别位点
MluI识别位点
MlyI识别位点
MmeI识别位点
MnlI识别位点
MscI识别位点
MseI识别位点
MslI识别位点
MspA1I
识别位点
MspI
识别位点
MwoI 识别位点
NaeI
识别位点
NarI
识别位点
NciI
识别位点
NcoI
识别位点
NdeI
识别位点
NgoMIV
识别位点
NheI
识别位点
NlaIII 识别位点
NlaIV 识别位点
NmeAIII 识别位点
NotI 识别位点
NruI 识别位点
NsiI 识别位点
NspI 识别位点
PacI
识别位点
PaeR7I 识别位点
PciI 识别位点
PflFI 识别位点
PflMI 识别位点
PhoI
识别位点
PleI
识别位点
PmeI 识别位点
PmlI
识别位点
PpuMI
识别位点
PshAI
识别位点
PsiI
识别位点
PspGI
识别位点
PspOMI
识别位点
PspXI
识别位点
PstI 识别位点
PvuI 识别位点
PvuII 识别位点
RsaI 识别位点
RsrII
识别位点
SacI 识别位点
SacII 识别位点
SalI 识别位点
SapI 识别位点
Sau3AI 识别位点
Sau96I 识别位点
SbfI 识别位点
ScaI 识别位点
ScrFI
识别位点
SexAI 识别位点
SfaNI 识别位点
SfcI
识别位点
SfiI
识别位点
SfoI 识别位点
SgrAI
识别位点
SmaI
识别位点
SmlI
识别位点
SnaBI 识别位点
SpeI 识别位点
SphI
识别位点
SspI 识别位点
StuI 识别位点
StyD4I
识别位点
StyI 识别位点
SwaI 识别位点
Taq αI
识别位点
TfiI 识别位点
TliI
识别位点
TseI 识别位点
Tsp45I识别位点
Tsp509I识别位点TspMI
识别位点
TspRI识别位点
Tth111I识别位点XbaI
识别位点XcmI
识别位点XhoI
识别位点
XmaI识别位点
XmnI
识别位点
ZraI识别位点
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