亚低温治疗对脓毒症小鼠血清可溶性髓样细胞触发受体1及炎症因子水平的影响

广"#$2021年1月第43卷第1期67

论著•基础研究

亚低温治疗对脓毒症小鼠血清可溶性髓样

细胞触发受体1及炎症因子水平的影响▲

石齐芳杨光耀李言鹏王树云盛鹰

(上海市浦东医院急危重医学科,上海市201399，电子邮箱:shiqifang456@)【摘要】目的探讨亚低温治疗对脓毒症小鼠血清可溶性髓样细胞触发受体1(s/REM-1)及炎症因子水平的影响。方法采用随机数字表法将84只BALB/t小鼠分为正常对照组、脓毒症组、亚低温组，每组28只。除正常对照组外，其余两组采用腹腔注射脂多糖的方法建立脓毒症小鼠模型。造模成功后亚低温组给予物理降温法降温，使小鼠肛温维持在34Z~36Z,其余两组不做处理。建模后6%12h检测小鼠血清肿瘤坏死因子+(TNF-w)%白细胞介素6(ILC)、sTREM-1水平，观察建模后12h各组小鼠的存活率。结果建模后6、12h,正常对照组、亚低温组、脓毒血症组小鼠血清TNF t、ILC%s/REM-1水平依次升高(均P<0.05)。建模后12h 正常对照组小鼠存活率高于其余两组(均P<0-05"，但亚低温组和脓毒症组存活率差异无统计学意义

(P>0.05)。结论亚低温治疗可以降低脓毒症小鼠血清sTREM-1及炎症因子水平#

【关键词】脓毒症；亚低温；小鼠；可溶性髓样细胞触发受体1;炎症因子

【中图分类号】R454.5&R631【文献标识码】A【文章编号】0253-4304(2021)01-0067-03

DOI：10.11675/j.Wsn.0253-4304.2021.01.16

Effect of mill hypothermia therapy on semm levels of soluble triggering

recentor expressed on myeloiO cell1and inflammatory factors in sersis micc

SHI Qi-fang,YANG Gcang-jao,LI Yan-peg,WANG Shc-jun,SHENG Ying

(Department cf Emergency and Critical Cars Medicit,Shanghai Pudong Hospital,Shanghai201399,China)

(Abstract)Objective To explore the eXect of mild hypothemiia therapy on serum levels of soluble tOggering receptor expressed on myeloid cell1(sTREMl)and inOammatoe O/Wo in sepsis mice.Methods Eighty-four BALBLcmoceweeedoeoded onionoemaeconieoegeoup,sepsosgeoup,moed hypoiheemoageoup us ong ihe eandom numbe e table method,with28mice in each goup.Except the nomial control group,the remaining too geups used the therapy of intrapeOtoneal injection of/popolysacchaOde to establish the mouse model of sepsis.After successful modeling,the milU hypotheonig group received physical cooling th—apy W keep their rectal temperature at34Z to36Z,and the remaining two goups did not oceWe any treatwent.Sio and12hour aher modeling,seem tumor n—rosis Oictor+(TNF干), interleukin6(IL-6)and sTREM-1levels of mice were detected,and the survival iho of mice in each group was obseeed12hours after modeling.Results Sio and12hours after modeling,serum TNF-w,IL-6and sTREM-10x11 increased in the order of the nomial control goup,the mild hypothemiia group and the sepsis geup(hl P<0.05)-Twelve hours after modeling,the sueivat rate in the nomial control geup was higher than that in the remaining too geups(hl P<0.05),but no statistically significant dWerenco in sueivat rate blw—n the mild hypothemiia geup and the sepsis geup(P>0.05)-Conclusion M/d hypothemiia therapy can reduce serum levels of sTREM-1and inOammatoe factors in sepsis mWe-

(Key words]Sepsis,M/d hypothemiia,Mouse,Soluble OiggeOng receptor expressed on myeloid colt1, InOammatoro factor

低温可以减缓机体的代谢，降低氧耗，保护线粒体功能，减少缺血缺氧所致的细胞凋亡以及自由基产生,抑制炎症反应［1］。脓毒症是宿主对感染反应失控的结果,对脓毒症患者采用低温疗法可能有一定的效果'2(。但有关亚低温用于治疗脓毒症效果的研究结果并不一致［3-4］，其中有临床研究显示亚低温并不能改善脓毒症/脓毒症休克患者的预后髓样细胞触发受体1(OiggeOng receptor expressed on myeloid cell1,TREM-1)是一种新型炎症激发细胞膜受体，在炎症触发和级联放大的反应中作用突出。可溶性

▲基金项目：上海市浦东新区卫生系统重点学科建设资助(PWZxk2017C0)；上海市浦东医院浦秀计划(PX201607)作者简介:石齐芳(1987-)，男，硕士，主治医师,研究方向:脓毒症的基础与临床。

通信作者:盛鹰(1982〜)，男，硕士，副主任医师，研究方向：脓毒症的基础与临床，电子邮箱:sweet191@ 。