罗格列酮上调高脂饮食老年大鼠骨骼肌GLUT4和PKB的表达

罗格列酮上调高脂饮食老年大鼠骨骼肌GLUT4和PKB的表
达
高宇;冯增斌;刘晓燕;王敬;马慧娟;宋光耀
【期刊名称】《中国老年学杂志》
【年(卷),期】2011(31)2
【摘要】Objective To observe the expressions of glucose transporter 4 (GLPT4) and protein kinase B (PKB) in high-fat-fed aged rats and the intervention effect of rosiglitazone. Methods Wistar rats were divided into control, high-fat diet (HF) and high-fat diet plus rosiglitazone maleate tablets (RSG) groups, 20 rats in each group. Insulin sensitivity was evaluated by conscious hyperinsulinemic-euglycemic clamp techniques, the expressions of GLUT4 mRNA and PKB mRNA and protein were analyzed by real time PCR and Western blot.Results Skeletal musclelong-chain fatty acyl coenzyme A (LCACoA) was significantly higher and glucose infusion rate was significantly lower in control group than those in HF group. The expressions of GLUT4 and PKB were decreased in HF group, RSG group reserves were abeve of all the changes. Conclusions The increased expressions of GLUT4 and PKB play a pivotal role in insulin-sensitizing effect of rosiglitazone treatment, it maybe the one of the mechanism of improving insulin resistance.%目的观察高脂饮食对老年大鼠骨骼肌葡萄糖转运蛋白4(GLUT4)和蛋白激酶B(PKB)的表达变化及罗格列酮的干预效果.方法老年大鼠随机分为对照组、高脂组(HF)、高脂+罗格列酮干预组(RSG),
每组20只.应用清醒状态下正常葡萄糖高胰岛素钳夹技术的葡萄糖输注率评价胰岛素抵抗,用荧光定量PCR法和Western印迹技术检测骨骼肌GLUT4和PKB的表达.结果高脂组骨骼肌长链脂酰辅酶A(LCACoA)升高而葡萄糖输注率明显下降(P＜0.01),骨骼肌GLUT4和PKB的表达明显降低(P＜0.05,P＜0.01),罗格列酮干预组显著缓解高脂组上述变化(P＜0.05,P＜0.01).结论罗格列酮上调高脂饮食老年大鼠骨骼肌GLUT4和PKB的表达,是改善老年胰岛素抵抗的机制之一.
【总页数】3页(P247-249)
【作者】高宇;冯增斌;刘晓燕;王敬;马慧娟;宋光耀
【作者单位】承德医学院附属医院,河北,承德,067000;承德医学院附属医院,河北,承德,067000;承德医学院附属医院,河北,承德,067000;河北省中医院;河北省人民医院;河北省人民医院
【正文语种】中文
【中图分类】R587.1
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