BLM18GG471SN1磁珠规格书
磁珠规格书
Remark
0.025
1
0.05 0.05 0.075 0.085 0.105 0.037 0.047 0.053 0.070 0.105 0.115 0.165 0.22 0.25 For DC power line
1400*
0.035 0.06 0.07 0.09 0.022 0.032 0.038 0.055 0.090 0.10 0.15 0.20 0.23
1700*1 1500* 1300*
1
1100*1 1000* 900*
1
1
1
3000*1 1700*1 2500*1 1400*1 2300*1 1300*1 2000*1 1100*1 1500*1 1400*1 1200*1 1000*1 900*1 800*1 800*1 700*1 600*1 500*1
Resistance element becomes dominant at high frequencies.
)
■ Unit Mass(Typical value) 0.001g
5.Marking
No marking.
MURATA MFG.CO.,LTD.
Remark
For high speed signal line
MURATA MFG.CO.,LTD.
Spec.No.JENF243A-0018AD-01
Reference Only
DC Resistance Impedance () (Note) ( max.) (at 100MHz,Under Standard Rated Current Testing Condition) (mA) Initial Values Values After Typical at 85℃ at 125℃ Testing 5～15 30±25% 60±25% 80±25% 120±25% 33±25％ 60±25％ 80±25％ 120±25％ 180±25％ 220±25％ 330±25％ 470±25％ 600±25％ 10 30 60 80 120 33 60 80 120 180 220 330 470 600 1000 2200*
磁珠选型参数
磁珠选型参数
磁珠的选型参数主要包括粒径、表面修饰和交叉频率。
1. 粒径：磁珠的粒径是指其直径大小，通常以纳米为单位表示。
粒径的选择取决于待分离物的大小和所需纯度。
一般而言，较小的粒径能提供更高的分辨率和更好的纯度，但可能会降低操作效率。
大多数应用中常用的磁珠粒径为50-200纳米。
2. 表面修饰：磁珠表面通常会进行修饰以增加其亲和性或特定功能。
例如，可以将氨基酸、抗体、核酸等物质固定在磁珠表面，以实现对特定分子的选择性结合。
选择合适的表面修饰可以提高磁珠的选择性和纯度。
3. 交叉频率：小于交叉频率时，Z和XL几乎是重合的，此时的磁珠主要呈感性，电感并不会吸收能量，此时反射噪声；大于交叉频率时，Z和R曲线几乎是重合的，此时磁珠主要呈电阻特性，大电阻，起吸收噪声并转变为热能的作用，此时才是体现磁珠的吸收噪声干扰的作用。
综上所述，在选择磁珠时，需要综合考虑这些参数以满足特定的应用需求。
请注意，对于具体的应用场景和需求，可能需要更多的实验和研究来确定最佳的磁珠选型参数。
BLM21AG102SN1磁珠规格书
EG
For GHz Band General Use (Low DC Resistance Type)
HB
HD
For GHz Band High-speed Signal Lines
HE
GA
For High-GHz Band High-speed Signal Lines
GG
For High-GHz Band General Use
Type DC Bias Characteristics Improved Type
Ferrite Bead Single Type
eDimensions (LgW)
Code
Dimensions (LgW)
03
0.6g0.3mm
15
1.0g0.5mm
18
1.6g0.8mm
21
2.0g1.25mm
31
0402
For High Speed Signal (Sharp impedance characteristics)
For Large Current For Standard
GHz Range
For High Speed Signal
For Standard (Low DC
Resistance Type)
1400±40%
1000±25%
2000±40%
1800±25%
2700±40%
120±25%
145 (Typ.)
220±25%
270 (Typ.)
120±25%
-
150±25%
-
220±25%
-
330±25%
-
470±25%
BLM31PG391SN1磁珠规格书
yElectrode Expressed by a letter.
Ex.)
Code
S/T
A
W
Electrode Sn Plating Au Plating
Ag/Pd
uCategory Code Z H
For Automotive
Category Infotainment
Powertrain, SafeSignal (Sharp impedance characteristics)
For Large Current For Standard
GHz Range
For High Speed Signal
For Standard (Low DC
Resistance Type)
Type DC Bias Characteristics Improved Type
Ferrite Bead Single Type
eDimensions (LgW)
Code
Dimensions (LgW)
03
0.6g0.3mm
15
1.0g0.5mm
18
1.6g0.8mm
21
2.0g1.25mm
31
Impedance (Ω)
at 100MHz
at 1GHz
10 (Typ.)
-
70 (Typ.)
-
120±25%
-
240±25%
-
10 (Typ.)
-
70 (Typ.)
-
120±25%
-
220±25%
-
600±25%
-
1000±25%
-
600±25%
磁珠选型参数
磁珠选型参数一、磁珠概述磁珠是一种电子元器件，主要用于滤波、耦合、旁路等电路中。
它能有效地抑制高频干扰信号，提高电路的稳定性。
在电子设备中，磁珠的应用越来越广泛，因此对磁珠的选型也显得尤为重要。
二、磁珠选型参数的重要性磁珠的选型参数决定了其性能和应用效果。
在进行磁珠选型时，需要关注以下几个关键参数：材质、尺寸、电阻、电感和频率响应。
这些参数直接影响到磁珠的使用效果，因此具有重要参考价值。
三、磁珠选型参数详解1.磁珠材质：常见的磁珠材质有铁氧体（Ferrite）、陶瓷（Ceramic）和金属（Metal）。
不同材质的磁珠具有不同的性能，如铁氧体磁珠具有较高的磁导率和较低的损耗，适用于高频信号处理；陶瓷磁珠则具有较高的电阻和电感，适用于电源滤波等场景。
2.磁珠尺寸：磁珠尺寸包括直径、长度和厚度。
尺寸越大，磁珠的电感和电阻越大，对高频信号的抑制能力越强。
但在实际应用中，需要根据电路空间和性能要求来选择合适的尺寸。
3.磁珠电阻：磁珠电阻决定了其对电流的阻碍程度。
在高频信号传输中，电阻越小，磁珠对高频信号的损耗越小。
因此，在选型时需要根据电路需求选择合适的电阻值。
4.磁珠电感：磁珠电感决定了其对交流信号的阻抗。
电感越大，磁珠对高频信号的抑制能力越强。
在选型时，需要根据电路的滤波需求来选择合适的电感值。
5.磁珠频率响应：磁珠频率响应是指磁珠在不同频率下的性能表现。
高频响应越好，磁珠对高频干扰的抑制能力越强。
在选型时，需要关注磁珠的频率响应曲线，确保其在所需频率范围内具有较好的性能。
四、选型实例分析以一款铁氧体磁珠为例，其尺寸为3mm×3mm×1.5mm，电阻为10Ω，电感为100nH，频率响应在100MHz以上。
这款磁珠适用于高频信号处理，如手机、通信设备等场景。
五、总结与建议磁珠选型是电子电路设计中的重要环节。
在选型时，要充分考虑磁珠的材质、尺寸、电阻、电感和频率响应等参数，以确保电路性能和稳定性。
BLM15HD182SN1磁珠规格书
BD
BX
PD
PG For Power Lines
PN
PX
KG For Power Lines (Low DC Resistance Type)
SG
HG
For GHz Band General Use
EB
For GHz Band High-speed Signal Lines (Low Direct Current Type)
Chip Ferrite Bead for Automotive Part Numbering
(Part Number)
BL M 18 AG 102 S Z 1 D q w e r t y u io
qProduct ID Product ID BL
Chip Ferrite Beads
wType Code E M
Impedance (Ω)
at 100MHz
at 1GHz
10 (Typ.)
-
70 (Typ.)
-
120±25%
-
240±25%
-
10 (Typ.)
-
70 (Typ.)
-
120±25%
-
220±25%
-
600±25%
-
1000±25%
-
600±25%
140 (Typ.)
1000±25%
300 (Typ.)
0402
For High Speed Signal (Sharp impedance characteristics)
For Large Current For Standard
GHz Range
For High Speed Signal
村田磁珠规格书
村田磁珠规格书
摘要：
一、村田磁珠简介
1.村田磁珠的定义与作用
2.产品分类与特点
二、村田磁珠规格书内容
1.产品型号与参数
2.外观尺寸与形状
3.磁性能参数
4.环境适应性
5.应用领域与注意事项
正文：
村田磁珠规格书详细介绍了村田磁珠的相关信息，包括产品简介和具体规格。
一、村田磁珠简介
村田磁珠是一种具有磁性的珠子，主要作用是用于电子设备的磁性元件。
它具有高磁导率、低磁滞损耗和良好的耐热性能，广泛应用于各种电子设备中。
根据产品特点和用途，村田磁珠可分为多种类型，如圆形磁珠、方形磁珠、异形磁珠等。
二、村田磁珠规格书内容
1.产品型号与参数
村田磁珠规格书中详细列出了各种型号的磁珠参数，包括尺寸、磁导率、饱和磁密等。
用户可以根据实际需求选择合适的型号。
2.外观尺寸与形状
规格书中还包括了村田磁珠的外观尺寸和形状，以便用户了解产品的外形特点和尺寸要求。
3.磁性能参数
磁性能参数是衡量磁珠性能的重要指标，规格书中详细列出了各种型号磁珠的磁性能参数，包括磁导率、剩磁、矫顽力等。
4.环境适应性
村田磁珠在不同的环境条件下，其性能和可靠性可能受到影响。
规格书中详细介绍了磁珠的环境适应性，包括耐热性、耐湿性、耐腐蚀性等，帮助用户了解产品在不同环境下的使用情况。
5.应用领域与注意事项
最后，规格书还列出了村田磁珠的应用领域和注意事项，帮助用户了解产品的适用范围和在使用过程中需要注意的问题，以确保产品能够安全、稳定地运行。
陶瓷贴片电容丶贴片电感丶片式磁珠命名规则与基本知识
陶瓷贴片电容、贴片电感.片式磁珠muRuta村田命名规则与基本知识一、村田陶瓷贴片电容知识M名衣示法如卜：片状独们盹瓷电容器GRM 15.3R7K 225KE15D•GRM—农示儀锡电极：卄通貼片期瓷12容•常用的村｝ 11容就是GRM艸通贴片海瓷电容与GNM卄通贴片搏容：•18——衣示尺寸（长*宽）:1.6*0.8mm即内通用尺对衣示是（K*审）1.6*0.8mm （单位为mm：c词阿卜通用尺、J A示是用英寸0603 （单位为inch；，卜J E询常用代码仃03、15、18、21、31、32、42、43、55〕；，II 体的对应值如卜I03-...0.6*0.3mm- (0201)15——1.0*0.5mm~-040218-...1.6*0.8mm- (0603)21-—2.0# 1.25mm——080531一“3.2* 1.6mm—120632-—3.2* 1.5mm一・121042一.5*2.0mm——180843 …45*3・2mm•—181255—5.7 拿5・0mm——2220•8——衣示用度（T：: 0.8mm常用厚度村田代码仃5、6、8、9、B、C、E：'h貝㈱对应值如卜：5-…0.5mm 6-…0.6mm 8-…0.8mm 9-…0.9mm B-…1.25mm C-…1.6mm E -—2.5mm•R7——农示材质：X7R常用材质村t：代码有5C、R6、R7、F5等,R体的对应值如卜：5C--COG/NPO/CHR6——X5RR7——X7RF5・——Y5V5C I仆温度是・55度一+125必温度系敌是0+・30ppm/gR6 I作泪度是・55度一H85度，SH度系数是+-15%:R7 1作温度是・55度一H25度,温度系数好+15%:F5 I作fiU是・30度—85度.温度系数是+22 -82%lOOpfW卜小容fi*L般采比5C材頂，100PF—luf人一般采用R7材质，luf以上一股采用R6材质,櫛S®求不禹的般采用F5材质。
BLM18HG471SN1磁珠规格书
Series BLM03/15/18/21 BLM03/15 BLM15/18 BLM03/15/18/21 BLM03/15 BLM03/15/18/21 BLM15 BLM15 BLM03/15/18/21/31/41 BLE32 BLM03/15
BLM18
BLM03/15/18 BLM03 BLM15/18 BLM03/15/18 BLM03/15/18 BLM18 BLM15 BLM15/18
470 470 470 470 470 470 470 420 420
400
330 330
330 330
240
220 220 220 220 220 220 220 220
200
150 150
150 150
140
120 120 120 120 120 120 120 120 120
100
70 70
75 75 75 60 60
200
100 500 700
500
300 500 200 300 200
200
Size (EIA Code)
Type
For High Speed Signal (Sharp impedance characteristics)
For Digital Interface
0603
For Large Current For Standard
Chip Ferrite Bead for Automotive Part Numbering
(Part Number)
BL M 18 AG 102 S Z 1 D q w e r t y u io
qProduct ID Product ID BL
BLM18AG102SN1磁珠规格书
Impedance (Ω)
at 100MHz
at 1GHz
10 (Typ.)
-
70 (Typ.)
-
120±25%
-
240±25%
-
10 (Typ.)
-
70 (Typ.)
-
120±25%
-
220±25%
-
600±25%
-
1000±25%
-
600±25%
140 (Typ.)
1000±25%
300 (Typ.)
Chip Ferrite Bead for Automotive Part Numbering
oPackaging
Code
Packaging
K
Embossed Taping (ø330mm Reel)
L
Embossed Taping (ø180mm Reel)
B
Bulk
For High Speed Signal
GHz Range
For Digital Interface
For Standard (Low DC
Resistance Type)
0805
For Standard
Part Number
BLM18BB151SN1 BLM18BD151SN1 BLM18BB221SN1 BLM18BD221SN1 BLM18BB331SN1 BLM18BD331SN1 BLM18BD421SN1 BLM18BB471SN1 BLM18BD471SN1 BLM18BD601SN1 BLM18BD102SN1 BLM18BD152SN1 BLM18BD182SN1 BLM18BD222SN1 BLM18BD252SN1 BLM18RK121SN1 BLM18RK221SN1 BLM18RK471SN1 BLM18RK601SN1 BLM18RK102SN1 BLM18PG300SN1 BLM18PG330SN1 BLM18PG600SN1 BLM18PG121SN1 BLM18PG181SN1 BLM18HG471SN1 BLM18HG601SN1 BLM18HG102SN1 BLM18HB121SN1 BLM18HB221SN1 BLM18HB331SN1 BLM18HD471SN1 BLM18HD601SN1 BLM18HD102SN1 BLM18HK331SN1 BLM18HK471SN1 BLM18HK601SN1 BLM18HK102SN1 BLM18EG101TN1 BLM18EG121SN1 BLM18EG221TN1 BLM18EG331TN1 BLM18EG391TN1 BLM18EG471SN1 BLM18EG601SN1 BLM18GG471SN1 BLM21AG121SN1 BLM21AG151SN1 BLM21AG221SN1 BLM21AG331SN1 BLM21AG471SN1 BLM21AG601SN1 BLM21AG102SN1
磁珠规格书
Part No.
CBW160808U110 CBW160808U190 CBW160808U260 CBW160808U310 CBW160808U800 CBW160808U101 CBW160808U121 CBW160808U151 CBW160808U181 CBW160808U221 CBW160808U301 CBW160808U501 CBW160808U601 CBW160808U801 CBW160808U102 CBW160808U122 CBW160808U152 CBW160808U182 CBW160808U202
CBW322513U190 CBW322513U260 CBW322513U310 CBW322513U600 CBW322513U800 CBW322513U121 CBW322513U151 CBW322513U181 CBW322513U221 CBW322513U301 CBW322513U501 CBW322513U601 CBW322513U801 CBW322513U102
Impedance(Ω)
At 100MHz 26±25% 31±25% 60±25% 80±25% 100±25% 150±25% 180±25% 200±25% 300±25% 500±25% 600±25% 800±25% 1000±25% 1200±25%
3225 型 TYPE
Part No.
Impedance(Ω)
At 100MHz 26±25% 31±25% 75±25% 90±25% 120±25% 150±25% 220±25% 300±25% 500±25% 600±25% 850±25%
4532 型 TYPE
BLM18AG121SN1磁珠规格书
For High Speed Signal
GHz Range
For Digital Interface
For Standard (Low DC
Resistance Type)
0805
For Standard
Part Number
BLM18BB151SN1 BLM18BD151SN1 BLM18BB221SN1 BLM18BD221SN1 BLM18BB331SN1 BLM18BD331SN1 BLM18BD421SN1 BLM18BB471SN1 BLM18BD471SN1 BLM18BD601SN1 BLM18BD102SN1 BLM18BD152SN1 BLM18BD182SN1 BLM18BD222SN1 BLM18BD252SN1 BLM18RK121SN1 BLM18RK221SN1 BLM18RK471SN1 BLM18RK601SN1 BLM18RK102SN1 BLM18PG300SN1 BLM18PG330SN1 BLM18PG600SN1 BLM18PG121SN1 BLM18PG181SN1 BLM18HG471SN1 BLM18HG601SN1 BLM18HG102SN1 BLM18HB121SN1 BLM18HB221SN1 BLM18HB331SN1 BLM18HD471SN1 BLM18HD601SN1 BLM18HD102SN1 BLM18HK331SN1 BLM18HK471SN1 BLM18HK601SN1 BLM18HK102SN1 BLM18EG101TN1 BLM18EG121SN1 BLM18EG221TN1 BLM18EG331TN1 BLM18EG391TN1 BLM18EG471SN1 BLM18EG601SN1 BLM18GG471SN1 BLM21AG121SN1 BLM21AG151SN1 BLM21AG221SN1 BLM21AG331SN1 BLM21AG471SN1 BLM21AG601SN1 BLM21AG102SN1
磁珠选型参数
磁珠选型参数1. 简介磁珠是一种常用的生物分离和纯化技术，广泛应用于生物医学研究、临床诊断和生产制造等领域。
磁珠选型参数是指在使用磁珠进行分离和纯化时，需要考虑的与磁珠性能相关的参数。
本文将详细介绍磁珠选型参数的相关内容。
2. 磁珠选择在选择合适的磁珠时，需要考虑以下几个关键参数：2.1 粒径磁珠的粒径是指其直径大小，通常以纳米为单位表示。
粒径的选择取决于待分离物的大小和所需纯度。
一般而言，较小的粒径能提供更高的分辨率和更好的纯度，但可能会降低操作效率。
大多数应用中常用的磁珠粒径为50-200纳米。
2.2 表面修饰磁珠表面通常会进行修饰以增加其亲和性或特定功能。
例如，可以将氨基酸、抗体、核酸等物质固定在磁珠表面，以实现对特定分子的选择性结合。
选择合适的表面修饰可以提高磁珠的选择性和纯度。
2.3 磁性磁珠的磁性是指其对外加磁场的响应能力。
高磁性的磁珠具有较强的吸附能力和迅速的分离速度，但也可能对待分离物造成较大的机械剪切力。
低磁性的磁珠则具有较弱的吸附能力和较慢的分离速度，但对待分离物影响较小。
根据实际需求，选择适当磁性的磁珠是非常重要的。
2.4 稳定性在使用过程中，磁珠需要保持良好的稳定性，不发生聚集、沉淀或颗粒损失等现象。
因此，选择具有良好稳定性且耐受多次反复洗涤、重悬和储存处理的磁珠是必要的。
3. 磁珠选型参数影响因素在确定合适的磁珠选型参数时，需要考虑以下几个主要影响因素：3.1 待分离物特性待分离物的特性对磁珠选型参数有直接影响。
例如，如果待分离物是蛋白质，可以选择具有亲和基团的磁珠；如果待分离物是核酸，可以选择具有亲和碱基或磷酸基团的磁珠。
3.2 样品来源和性质样品来源和性质也会对磁珠选型参数产生影响。
例如，如果样品是血液、组织或细胞等复杂样品，可能需要选择具有更高选择性和较大表面积的磁珠。
3.3 分离纯度要求不同的应用对分离纯度有不同的要求。
某些应用可能对纯度要求较高，需要选择具有更好选择性和较小粒径的磁珠；而其他应用则对纯度要求相对较低。
BLM15HG102SN1磁珠规格书
For High Speed Signal
GHz Range
For Digital Interface
For Standard (Low DC
Resistance Type)
0805
For Standard
Part Number
BLM18BB151SN1 BLM18BD151SN1 BLM18BB221SN1 BLM18BD221SN1 BLM18BB331SN1 BLM18BD331SN1 BLM18BD421SN1 BLM18BB471SN1 BLM18BD471SN1 BLM18BD601SN1 BLM18BD102SN1 BLM18BD152SN1 BLM18BD182SN1 BLM18BD222SN1 BLM18BD252SN1 BLM18RK121SN1 BLM18RK221SN1 BLM18RK471SN1 BLM18RK601SN1 BLM18RK102SN1 BLM18PG300SN1 BLM18PG330SN1 BLM18PG600SN1 BLM18PG121SN1 BLM18PG181SN1 BLM18HG471SN1 BLM18HG601SN1 BLM18HG102SN1 BLM18HB121SN1 BLM18HB221SN1 BLM18HB331SN1 BLM18HD471SN1 BLM18HD601SN1 BLM18HD102SN1 BLM18HK331SN1 BLM18HK471SN1 BLM18HK601SN1 BLM18HK102SN1 BLM18EG101TN1 BLM18EG121SN1 BLM18EG221TN1 BLM18EG331TN1 BLM18EG391TN1 BLM18EG471SN1 BLM18EG601SN1 BLM18GG471SN1 BLM21AG121SN1 BLM21AG151SN1 BLM21AG221SN1 BLM21AG331SN1 BLM21AG471SN1 BLM21AG601SN1 BLM21AG102SN1
磁珠简介!磁珠命名规则及功能原理
磁珠简介!磁珠命名规则及功能原理
1.磁珠简介
村田（muRata）磁珠属于EMI静噪元件,也同属于静噪滤波器，学名叫片状铁氧体磁珠，功效等效于电阻和电感串联在电路中，阻值和电感值都由电路中频率变化而变化，在有高频通过时，表现出阻性，从而发挥出过滤高频的滤波器作用。
生产村田磁珠主要原料是铁氧体，主要成分是铁镁合金或铁镍合金，外表成灰黑色。
电路中的高频信号在通过铁氧体这种材料时会大量消耗高频信号，正是因为铁氧体的这种消耗高频的特性，使得磁珠在电路中起着阻高频通低频的作用。
2.磁珠命名规则
下图以型号BLM18AG331SN1D为例说明磁珠的命名规则
3.磁珠特性参数
（1）直流电阻DCResistance(mohm)：直流电流通过此磁珠时，此磁珠所呈现的电阻值。
（2）额定电流RatedCurrent(mA)：表示磁珠正常工作时的最大允许电流。
（3）阻抗[Z]@100MHz(ohm)：这里所指的是交流阻抗。
（4）阻抗-频率特性：描述阻抗值随频率变化的曲线。
（5）电阻-频率特性：描述电阻值随频率变化的曲线
（6）感抗-频率特性：描述感抗随频率变化的曲线
4.磁珠结构
当导线中电流穿过时，铁氧体对低频电流几乎没有什么阻抗，而对较高频率的电流会产生较大衰减作用。
高频电流在其中以热量形式散发，其等效电路为一个电感和一个电阻串联，两个组件的值都与磁珠的长度成比例。
磁珠种类很多，制造商应提供技术指标说明，特别是磁珠的阻抗与频率关系的曲线。
有的磁珠上有多个孔洞，用导线穿过可增加组件阻抗(穿过磁珠次数的平方)，不过在高频时所增加的抑制噪声能力不可能如预期的多，而用多串联几个磁珠的办法会好些。
链霉亲和素纳米磁珠说明书
版本2022/09/08（02）
链霉亲和素纳米磁珠（100-300nm ）说明书1/1链霉亲和素纳米磁珠（100-300nm ）产品说明书
【产品名称】链霉亲和素纳米磁珠（100-300nm ）
【英文名称】Streptavidin Nano Magnetic Beads (100-300nm)
【订货信息】
纳米磁珠/纯水【简介】
东纳生物科技有限公司提供链霉亲和素纳米磁珠（100-300nm ），链霉亲和素磁珠可与生物素化的多肽、蛋白、抗体等生物配体高效偶联，应用于免疫分析、基因分析和纯化PCR 产物等。
【产品参数】
图1：100nm 链霉亲和素磁珠扫描电镜（左图，100±10nm ）、200nm 链霉亲和素磁珠扫描电镜（中图，
220±30nm ）以及300nm 链霉亲和素磁珠扫描电镜（右图，310±20nm ）。
【注意事项】
1.磁珠取用前应充分混匀，防止取用改变磁珠浓度，避免长时间超声对磁珠表面破坏；
2.磁珠取用后，使用前请进行磁分离并用纯水或所用缓冲溶液清洗2-3遍；
3.磁珠使用和保存过程中应避免冻融。
【生产单位】
公司名称
南京东纳生物科技有限公司地址
南京市江宁区龙眠大道568号南京生命科技小镇5号楼北楼6楼邮政编码
210000电话号码
025********电子邮箱
**************公司网站。
磁珠法血液DNA提取试剂盒-说明书
血液DNA提取试剂盒（磁珠法）
【简介】
血液DNA提取试剂盒（磁珠法）适用血液的基因组DNA提取，尤其适用于人类以及哺乳动物的血液基因组DNA提取。
配合核酸自动纯化仪（GNT-SI系列），可实现一键式、自动化操作
【组分】
【血液DNA提取试剂盒（磁珠法）操作说明】（以实际说明书为准）
1、取抗凝全血到离心管中，加入Buffer LB1、Buffer LB2，混合均匀后，将离心管置于水浴
锅中，水浴30min
2、将离心管取出，加入异丙醇，Magnetic Beads。
颠倒混匀数次（期间应静置数秒后继续颠
倒混匀），将离心管置于磁力架，磁分离，吸弃废液
3、将离心管从磁力架上取下，加入Buffer WB I。
颠倒混匀数次（期间应静置数秒后继续颠
倒混匀），将离心管置于磁力架，磁分离，吸弃废液
4、将离心管从磁力架上取下，加入Buffer WB II，颠倒混匀数次（期间应静置数秒后继续颠
倒混匀），将离心管置于磁力架，磁分离，吸弃废液
5、重复步骤5一次，并将废液完全吸弃干净
6、将离心管从磁力架上取下，室温下开盖静置5min
7、加入Elution Buffer，磁珠完全混匀后，置于水浴锅中，水浴10min
8、将离心管置于磁力架，磁分离，小心吸取上清至新的离心管中，进行下游实验
【特点】
1、得率高：200ul人类抗凝全血的普遍产量可达3-8ug
2、纯度高：OD260/280稳定在左右，OD260/230稳定在以上
3、操作简便：手工提取过程无需离心
4、自动化：具有成熟的自动化方案，可配合核酸自动纯化仪，实现一键式操作
【提取效果】。
免疫磁珠说明书
Contents1. Description1.1 Principle of the MACS® Separation1.2 Background information1.3 Applications1.4 Reagent and instrument requirements试剂和仪器的要求2. Protocol2.1 Sample preparation样品制备2.2 Magnetic labeling磁性标记2.3 Magnetic separation磁性分离3. Example of a separation using the CD133 MicroBead Kit4. References1. DescriptionComponents 2 mL CD133 MicroBeads, human:MicroBeads conjugated to monoclonal antihumanCD133 antibodies (isotype: mouse IgG1,clone AC133).2 mL FcR Blocking Reagent, human Specificity CD133 antigen, epitope (CD133/1)1.Capacity For 2亊10⁹total cells, up to 100 separations.Product format CD133 MicroBeads are supplied in buffercontaining stabilizer and 0.05% sodium azide.Storage Store protected from light at 2−8 °C. Do not freeze. The expiration date is indicated on the vial label.1.1 Principle of the MACS® SeparationFirst, the CD133+ cells are magnetically labeled with CD133MicroBeads. Then, the cell suspension is loaded onto a MACS®Column, which is placed in the magnetic field of a MACS Separator.The magnetically labeled CD133+ cells are retained within thecolumn. The unlabeled cells run through; this cell fraction isthus depleted of CD133+ cells. After removing the column fromthe magnetic field, the magnetically retained CD133+ cells can beeluted as the positively selected cell fraction. To increase the purity,the positively selected cell fraction containing the CD133+ cells isseparated over a second column.1.2 Background informationThe CD133 MicroBead Kit is a magnetic labeling system designedfor the positive selection of CD133+ cells. It allows the single-stepisolation of nonhematopoietic and early hematopoietic progenitors and stem cells. The CD133 molecule is a 5-transmembrane cellsurface antigen with a molecular weight of 117 kD.2 The CD133/1(clone AC133) antibody recognizes epitope 1 of the CD133 antigen.1In the hematopoietic system, CD133 expression is restricted to asubset of CD34bright stem and progenitor cells in human fetal liver,bone marrow, cord blood, and peripheral blood.3 Isolated fromhematopoietic sources, CD133+ cells can become adherent and arereported to become CD133–during culture⁴. The CD34+CD133+cell population, which includes CD34+CD38–cells, was shown tobe capable of repopulating NOD/SCID mice.⁵Recently, CD133has also been found to be expressed on circulating endothelialprogenitor cells⁶,⁷and fetal neural stem cells⁸,⁹as well as on othertissue-specific stem cells, such as renal1⁰and prostate11 stem cells.Lately, when isolated from tumor tissue, the CD133+ populationcan be enriched for tumor-initiating cells.11-1⁵CD133 MicroBeadshave been used to isolate adult stem cells from cord blood and asa starting population for reprograming towards iPS cells.1⁶CD133expression has been found on undifferentiated human ES cells.Therefore CD133 MicroBeads could be used for enrichment ordepletion of these cells.1⁷1.3 Applications●Positive selection or depletion of cells expressing human CD133antigen.●Isolation or depletion of CD133+ cells from peripheral bloodmononuclear cells (PBMCs) or single-cell suspensions fromtissue.●CD133+ cells are used in basic stem cell research, stem cellevaluation, stem cell expansion, research in hematologicalmalignancies, stem cell plasticity, and potential cellulartherapies as well as in tissue regeneration and cancer research.1.4 Reagent and instrument requirements●Buffer: Prepare a solution containing phosphate-buffered saline(PBS), pH 7.2, 0.5% bovine serum albumin (BSA), and 2 mMEDTA by diluting MACS BSA Stock Solution (# 130‑091-376)1:20 with autoMACS Rinsing Solution (# 130-091-222). Keepbuffer cold (2−8 °C). Degas buffer before use, as air bubblescould block the column.▲Note: EDTA can be replaced by other supplements such as anticoagulant citrate dextrose formula-A (ACD-A) or citrate phosphate dextrose (CPD). BSA can be replaced by other proteins such as human serum albumin, human serum, or fetal bovine serum (FBS). Buffers or media containing Ca2+ or Mg2+ are not recommended for use.●(Optional) Fluorochrome-conjugated antibodies forflow cytometric analysis, e.g., CD133/2 (293C3)-PE(# 130-090-853), CD133/2 (293C3)-APC (# 130-090-854),CD133/2 (293C3)‑Biotin, CD34-FITC (# 130-081-001),CD34‑APC (# 130-090-954), or CD34-PE (# 130-081-002). Formore information about fluorochrome-conjugated antibodiessee .●MACS Columns and MACS Separators: CD133+ cells can beEnriched浓缩by using MS, LS, or XS Columns or depleted withthe use of LD, CS, or D Columns. Cells which strongly expressthe CD133 antigen can also be depleted using MS, LS, or XSColumns. Positive selection正向or depletion can also be performedby using the autoMACS Pro or the autoMACS Separator.▲Note: Column adapters are required to insert certain columns into theV arioMACS™or SuperMACS™Separators. For details see the respective MACS Separator data sheet.●(Optional) Propidium Iodide Solution (# 130-093-233) or7-AAD for flow cytometric exclusion of dead cells.●(Optional) Dead Cell Removal Kit (# 130-090-101) for thedepletion of dead cells.●(Optional) Pre-Separation Filters (# 130-041-407) to removecell clumps团.2. Protocol2.1 Sample preparationWhen working with anticoagulated peripheral blood or buffy coat,peripheral blood mononuclear cells (PBMCs) should be isolated bydensity gradient centrifugation, for example, using Ficoll-Paque™.▲Note: To remove platelets after density gradient separation, resuspend cell pellet in buffer and centrifuge at 200×g for 10−15 minutes at 20 °C. Carefully aspirate supernatant. Repeat washing step.When working with tissues or lysed blood, prepare a single-cellsuspension using standard methods.For details see the protocols section at /protocols.For preparation of cord blood cells, bone marrow cells, or cellsfrom leukapheresis material, please refer to the sample preparationprotocols at /protocols.▲Dead cells may bind non-specifically to MACS MicroBeads.To remove dead cells, we recommend using density gradientcentrifugation or the Dead Cell Removal Kit (# 130-090-101).2.2 Magnetic labeling▲Work fast, keep cells cold, and use pre-cooled solutions. This willprevent capping of antibodies on the cell surface and non-specificcell labeling.▲V olumes for magnetic labeling given below are for up to10⁸total cells. When working with fewer than 10⁸cells, use the same volumes as indicated. When working with higher cell numbers,scale up all reagent volumes and total volumes accordingly (e.g.for 2亊10⁸total cells, use twice the volume of all indicated reagent volumes and total volumes).▲For optimal performance it is important to obtain a single‑cell suspension before magnetic labeling. Pass cells through 30 μm nylonmesh (Pre-Separation Filters, # 130-041-407) to remove cell clumpswhich may clog the column. Moisten filter with buffer before use.▲The recommended incubation temperature is 2–8 °C. Workingon ice may require increased incubation times. Higher temperaturesand/or longer incubation times may lead to non-specific celllabeling.1. Determine确定cell number.2. Centrifuge cell suspension细胞悬液at 300×g for 10 minutes. Aspirate supernatant completely.吸取上清3. Resuspend cell pellet in 300 μL of buffer per 10⁸total cells.4. Add 100 μL of FcR Blocking Reagent per 10⁸total cells.5. Add 100 μL of CD133 MicroBeads per 10⁸total cells.6. Mix well and incubate for 30 minutes in the refrigerator(2−8 °C).7. (Optional) Add staining antibodies, e.g., 50 μL of CD133/2(293C3)-PE (# 130-090-853), and incubate for 5 minutes in thedark in the refrigerator (2−8 °C).8. Wash cells by adding 1−2 mL of buffer per 10⁸cells andcentrifuge at 300×g for 10 minutes. Aspirate supernatantcompletely.9. Resuspend up to 10⁸cells in 500 μL of buffer.▲Note: For higher cell numbers, scale up buffer volume accordingly.▲Note: For depletion with LD Columns, resuspend up to 1.25亊10⁸cells in 500 μL of buffer.10. Proceed to magnetic separation (2.3).2.3 Magnetic separation▲Choose an appropriate MACS Column and MACS Separatoraccording to the number of total cells and the number of CD133+cells. For details see table in section 1.4.▲Always wait until the column reservoir is empty before proceedingto the next step.Magnetic separation with MS or LS Columns▲To achieve highest purities, perform two consecutive columnruns.1. Place column in the magnetic field of a suitable MACS Separator.For details see the respective MACS Column data sheet.2. Prepare column by rinsing with the appropriate amount ofbuffer:MS: 500 μL LS: 3 mL3. Apply cell suspension onto the column. Collect flow-throughcontaining unlabeled cells.4. Wash column with the appropriate amount of buffer. Collectunlabeled cells that pass through and combine with the effluentfrom step 3.MS: 3×500 μL LS: 3×3 mL▲Note: Perform washing steps by adding buffer aliquots only when the column reservoir is empty.5. Remove column from the separator and place it on a suitablecollection tube.▲Note: To perform a second column run, you may elute the cells directly from the first onto the second, equilibrated column instead of a collection tube.6. Pipette the appropriate amount of buffer onto the column.Immediately flush out the magnetically labeled cells by firmlypushing the plunger into the column.MS: 1 mL LS: 5 mL7. To increase purity of CD133+ cells, enrich the eluted fractionover a second MS or LS Column. Repeat the magneticseparation procedure as described in steps 1 to 6 by using anew column.Magnetic separation with XS ColumnsFor instructions on the column assembly and the separation refer tothe XS Column data sheet.Depletion with LD Columns1. Place LD Column in the magnetic field of a suitable MACSSeparator. For details see LD Column data sheet.2. Prepare column by rinsing with 2 mL of buffer.3. Apply cell suspension onto the column.4. Collect unlabeled cells that pass through and washcolumn with 2×1 mL of buffer. Collect total effluent;this is the unlabeled cell fraction. Perform washingsteps by adding buffer two times. Only add new buffer whenthe column reservoir is empty.Depletion with CS Columns1. Assemble CS Column and place it in the magnetic field of asuitable MACS Separator. For details see CS Column datasheet.2. Prepare column by filling and rinsing with 60 mL of buffer.Attach a 22G flow resistor to the 3-way stopcock of theassembled column. For details see CS Column data sheet.3. Apply cell suspension onto the column.4. Collect unlabeled cells that pass through and wash columnwith 30 mL buffer from the top. Collect total effluent; this isthe unlabeled cell fraction.Depletion with D ColumnsFor instructions on column assembly and separation refer to theD Column data sheet.Magnetic separation with the autoMACS® Pro Separator or theautoMACS® Separator▲Refer to the respective user manual for instructions on how to use the autoMACS Pro Separator or the autoMACS Separator.▲Buffers used for operating the autoMACS Pro Separator or the autoMACS Separator should have a temperature of ≥10 °C.▲Program choice depends on the isolation strategy, the strengthof magnetic labeling, and the frequency of magnetically labeled cells. For details refer to the section describing the cell separation programs in the respective user manual.Magnetic separation with the autoMACS® Pro Separator1. Prepare and prime the instrument.2. Apply tube containing the sample and provide tubes for collecting the labeled and unlabeled cell fractions. Placesample tube in row A of the tube rack and the fractioncollection tubes in rows B and C.3. For a standard separation choose one of the following programs:Positive selection from peripheral blood, bone marrow, or leukapheresis: “Posseld”Collect positive fraction in row C of the tube rack.Positive selection from cord blood: “Posseld2”Collect positive fraction in row C of the tube rack.Depletion: “Depletes”Collect negative fraction in row B of the tube rack.Magnetic separation with the autoMACS® Separator1. Prepare and prime the instrument.2. Apply tube containing the sample and provide tubes for collecting the labeled and unlabeled cell fractions. Placesample tube at the uptake port and the fraction collectiontubes at port neg1 and port pos 2.3. For a standard separation choose one of the following programs:Positive selection from peripheral blood, bone marrow, or leukapheresis: “Posseld”Collect positive fraction from outlet port pos 2.Positive selection from cord blood: “Posseld2”Collect positive fraction from outlet port pos 2.Depletion: “Depletes”Collect negative fraction from outlet port neg1.3. Example of a separation using the CD133MicroBead KitCD133+ hematopoietic stem and progenitor cells were isolated from non-mobilized human PBMCs using the CD133 MicroBead Kit, MS Columns, and a MiniMACS™Separator. Cells were fluorescently stained with CD34-FITC (# 130-081-001) and CD133/2 (293C3)-PE (# 130-090-853) and analyzed by flow cytometry. Cell debris and dead cells werde excluded from the analysis based on scatter signals and propidium iodide fluorescence.All protocols and data sheets are available at .WarningsReagents contain sodium azide. Under acidic conditions sodium azide yields hydrazoic acid, which is extremely toxic. Azide compounds should be diluted with running water before discarding. These precautions are recommended to avoid deposits in plumbing where explosive conditions may develop.WarrantyThe products sold hereunder are warranted only to be free from defects in workmanship and material at the time of delivery to the customer. Miltenyi Biotec GmbHmakes no warranty or representation, either expressed or implied, with respect tothe fitness of a product for a particular purpose. There are no warranties, expressedor implied, which extend beyond the technical specifications of the products.Miltenyi Biotec GmbH’s liability is limited to either replacement of the products or refund of the purchase price. Miltenyi Biotec GmbH is not liable for any property damage, personal injury or economic loss caused by the product.autoMACS and MACS are registered trademarks and MidiMACS, MiniMACS, OctoMACS, QuadroMACS, SuperMACS, and V arioMACS are trademarks of Miltenyi Biotec GmbH.Ficoll-Paque is a trademark of GE Healthcare companies.Copyright . 2009 Miltenyi Biotec GmbH. All rights reserved.。
BLM18PG600SN1磁珠规格书
1400±40%
1000±25%
2000±40%
1800±25%
2700±40%
120±25%
145 (Typ.)
220±25%
270 (Typ.)
120±25%
-
150±25%
-
220±25%
-
330±25%
-
470±25%
-
600±25%
-
1000±25%
-
5±25%
-
10±25%
-
22±25%
Impedance (Ω)
at 100MHz
at 1GHz
10 (Typ.)
-
70 (Typ.)
-
120±25%
-
240±25%
-
10 (Typ.)
-
70 (Typ.)
-
120±25%
-
220±25%
-
600±25%
-
1000±25%
-
600±25%
140 (Typ.)
1000±25%
300 (Typ.)
yElectrode Expressed by a letter.
Ex.)
Code
S/T
A
W
Electrode Sn Plating Au Plating
Ag/Pd
uCategory Code Z H
For Automotive
Category Infotainment
Powertrain, Safety
200
100 500 700
500
300 500 200 300 200
200
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1400±40%
1000±25%
2000±40%
1800±25%
2700±40%
120±25%
145 (Typ.)
220±25%
270 (Typ.)
120±25%
-
150±25%
-
220±25%
-
330±25%
-
470±25%
-
600±25%
-
1000±25%
-
5±25%
-
10±25%
-
22±25%
For Standard
0603
For High Speed Signal (Sharp impedance characteristics)
Part Number
BLM03AG100SN1 BLM03AG700SN1 BLM03AG121SN1 BLM03AG241SN1 BLM15AG100SN1 BLM15AG700SN1 BLM15AG121SN1 BLM15AG221SN1 BLM15AG601SN1 BLM15AG102SN1 BLM15AG601AN1 BLM15AG102AN1 BLM15BB050SN1 BLM15BB100SN1 BLM15BB220SN1 BLM15BB470SN1 BLM15BB750SN1 BLM15BB121SN1 BLM15BB221SN1 BLM15BD750SN1 BLM15BD121SN1 BLM15BD221SN1 BLM15BD471SN1 BLM15BD601SN1 BLM15BD102SN1 BLM15BD182SN1 BLM15PG100SN1 BLM15HG601SN1 BLM15HG102SN1 BLM15HD601SN1 BLM15HD102SN1 BLM15HD182SN1 BLM15EG121SN1 BLM15EG221SN1 BLM18AG121SN1 BLM18AG151SN1 BLM18AG221SN1 BLM18AG331SN1 BLM18AG471SN1 BLM18AG601SN1 BLM18AG102SN1 BLM18BA050SN1 BLM18BB050SN1 BLM18BA100SN1 BLM18BB100SN1 BLM18BA220SN1 BLM18BB220SN1 BLM18BA470SN1 BLM18BB470SN1 BLM18BB600SN1 BLM18BA750SN1 BLM18BB750SN1 BLM18BA121SN1 BLM18BB121SN1 BLM18BD121SN1 BLM18BB141SN1
60 (0.5A) 60 (3A)
50 (3A)
60 (6A)
33 (3A) 30 (1A)
30 (3A)
33 (6A)
22 22
10 10 10
10 10
555
mm 0603 1005 1608 2012 1005 1608 2012 1608 2012
EIA Code 0201 0402 0603 0805 0402 0603 0805 0603 0805
Type DC Bias Characteristics Improved Type
Ferrite Bead Single Type
eDimensions (LgW)
Code
Dimensions (LgW)
03
0.6g0.3mm
15
1.0g0.5mm
18
1.6g0.8mm
21
2.0g1.25mm
31
-
47±25%
-
60±25%
-
75±25%
-
-
120±25%
-
-
140±25%
-
BLM Series
Rated Current (mA) 500 200 200 100 1000 500
300 200 300 200 500
300
200
300
200
100 1000 300 250 300 250 200 1500* 700*
For Standard BLMppA/T
For High Speed For Digital
Signal
Interface
BLMppB BLMppR
10 (1A)
1005 0402
22 (6A)
1608
2012
3216
0603
0805
1206
For Large Current BLMppP
( )=Rated Current
Chip Ferrite Bead for Automotive Part Numbering
(Part Number)
BL M 18 AG 102 S Z 1 D q w e r t y u io
qProduct ID Product ID BL
Chip Ferrite Beads
wType Code E M
3.2g1.6mm
32
3.2g2.5mm
41
4.5g1.6mm
EIA 0201 0402 0603 0805 1206 1210 1806
tImpedance Expressed by three figures. The unit is in ohm (Ω) at 100MHz. The first and second figures are significant digits, and the third figure expresses the number of zeros that follow the two figures.
4516 1806
1005
1608
0402
0603
GHz Range Noise GHz Range Noise Suppression Type Suppression Type BLM15H/E BLM18H/E/G
Size (EIA Code) 0201
Type For Standard
For Standard
200
100 500 700
500
300 500 200 300 200
200
Size (EIA Code)
Type
For High Speed Signal (Sharp impedance characteristics)
For Digital Interface
0603
For Large Current For Standard
Series BLM03/15/18/21 BLM03/15 BLM15/18 BLM03/15/18/21 BLM03/15 BLM03/15/18/21 BLM15 BLM15 BLM03/15/18/21/31/41 BLE32 BLM03/15
BLM18
BLM03/15/18 BLM03 BLM15/18 BLM03/15/18 BLM03/15/18 BLM18 BLM15 BLM15/18
0402
For High Speed Signal (Sharp impedance characteristics)
For Large Current For Standard
GHz Range
For High Speed Signal
For Standard (Low DC
Resistance Type)
Chip Ferrite Bead for Automotive Part Numbering
oPackaging
Code
Packaging
K
Embossed Taping (ø330mm Reel)
L
Embossed Taping (ø180mm Reel)
B
Bulk
BD
BX
PD
PG For Power Lines
PN
PX
KG For Power Lines (Low DC Resistance Type)
SG
HG
For GHz Band General Use
EB
For GHz Band High-speed Signal Lines (Low Direct Current Type)
For High Speed Signal
GHz Range
For Digital Interface
For Standard (Low DC
Resistance Type)
0805
For Standard
Part Number
BLM18BB151SN1 BLM18BD151SN1 BLM18BB221SN1 BLM18BD221SN1 BLM18BB331SN1 BLM18BD331SN1 BLM18BD421SN1 BLM18BB471SN1 BLM18BD471SN1 BLM18BD601SN1 BLM18BD102SN1 BLM18BD152SN1 BLM18BD182SN1 BLM18BD222SN1 BLM18BD252SN1 BLM18RK121SN1 BLM18RK221SN1 BLM18RK471SN1 BLM18RK601SN1 BLM18RK102SN1 BLM18PG300SN1 BLM18PG330SN1 BLM18PG600SN1 BLM18PG121SN1 BLM18PG181SN1 BLM18HG471SN1 BLM18HG601SN1 BLM18HG102SN1 BLM18HB121SN1 BLM18HB221SN1 BLM18HB331SN1 BLM18HD471SN1 BLM18HD601SN1 BLM18HD102SN1 BLM18HK331SN1 BLM18HK471SN1 BLM18HK601SN1 BLM18HK102SN1 BLM18EG101TN1 BLM18EG121SN1 BLM18EG221TN1 BLM18EG331TN1 BLM18EG391TN1 BLM18EG471SN1 BLM18EG601SN1 BLM18GG471SN1 BLM21AG121SN1 BLM21AG151SN1 BLM21AG221SN1 BLM21AG331SN1 BLM21AG471SN1 BLM21AG601SN1 BLM21AG102SN1