生物化学与分子生物学PPT图片素材

Pantetheine
NADPH NADP
NADP
NADPH
Pantetheine
Cholesterol de novo synthesis I
Isoprenyl formation
2 NADPH 2 NADP
Thiolase
Acetoacetyl-CoA
HMG-CoA synthase
3-Hydroxy-3methylglutaryl-CoA
Nicotinamide
NAD NAD
Poly(ADP ribosyl) polymerase (PARP)
DNA polymerase
Poly(ADP ribosyl) polymerase (PARP) Nicotinamide
DNA polymerase
Poly(ADP ribosyl) chain elongation and branching
40S 60S
Pyrimidine Nucleotide Interconversions
ADP ADP ADP ATP
UMP
ATP
UDP
ATP
UTP
CTP
H2O
Nucleoside monophosphate kinase
Nucleoside diphosphate kinase
CTP synthase
生物化学与分子生物学 PPT素材
可根据需要选择取舍
Metabolism of 5-fluorouracil (5-FU)
Thymidine phosphorylase
Uridine phosphorylase
5-FU
Carac, Efudex
5-Fluorodeoxyuridine Orotate phosphoribosyl transferase ATP Uridine kinase ADP PRPP ATP
Carnitine acyltransferase I
Palmitoyl carnitine Acetyl-CoA
Myristoyl CoA Palmitoyl CoA Palmitoyl CoA
Carnitine acyltransferase II
AMP
Palmitoyl CoA ligase
Palmitoyl CoA
Ins-1,4,5-P3
PTEN
Ins-1,3,4-P3
PTEN
Ins-1,4-P3
Fatty acids synthesis
Acetyl CoA carboxylase
Oxaloacetate
Malonyl CoA
Citrate lyase ATP
Acetyl CoA
Citrate
ADP + Pi
Myo-Inositol-1,3,4,5,6-pentaphosphate Ins(1,3,4,5,6)P5
Myo-Inositol-1,2,3,4,5-pentaphosphate Ins(1,2,3,4,5)P5
Myo-Inositol-1,2,4,5,6-pentaphosphate Ins(1,2,4,5,6)P5
Ribonucleotide reductase Nucleoside diphosphate kinase Nucleoside TTP TMP monophosphate kinase
DHF
deoxyUDP
N5,N10-MethyleneTHF
deoxyUMP
Nucleoside diphosphate phosphohydrolase
5-Cholestan-3, 7, 12-Triol
Sterol 27hydroxylase CYP27A1
7, 12-Dihydroxy-4-cholesten-3-one 5-Cholestan-3, 7-diol
Cholic acid 3, 7, 12-Trihydroxy-5-cholestanoic acid
HMG-CoA reductase
3, 3-Dimethylalylpyrophosphate
Mevalonate kinase
Mevalonate
Isomerase ADP
ATP
Mevalonate5-pyrophospho decarboxylase
Phosphomevalona来自百度文库e kinase
NAD
DNA polymerase
N-glycosylation pathway in ER
P P Dolichol
GDP-Mannosyl transferases
P P Dolichol
GlcNAc transferase
P P Dolichol
GlcNAc-P transferase
Cytosol
ATP ATP
3-Isopentenylpyrophosphate
ADP
5-Pyrophosphomevalonate
ADP
5-Phosphomevalonate
Biosynthesis of bile acids
Cholesterol
Sterol 7 hydroxylase CYP7A1
3-Hydroxy stroid dehydrogenase HSD3B7
7-Hydroxycholesterol
7-Hydroxy-4-cholesten-3-one
Sterol-12hydroxylase CYP8B1 AKR1D1 AKR1C4
3-Hydroxysteroid dehydrogenase AKR1C4 Oxosteroid5-reductase AKR1D1
Myo-Inositol-1,3,4-triphosphate Ins(1,3,4,)P3
Myo-Inositol-1,4,5-triphosphate Ins(1,4,5)P3
Myo-Inositol-3,4,5,6-tetraphosphate Ins(3,4,5,6)P4
Myo-Inositol-1,3,4,6-tetraphosphate Ins(1,3,4,6)P4
Myo-Inositol-1,2,3,4,5,6-hexaphosphate Ins(1,2,3,4,5,6)P6 (Phytic acid)
Phospho-myo-inositol Toolkit
Myo-Inositol-3-phosphate Ins(3)P1 Myo-Inositol-3,4-diphosphate Ins(3,4)P2 Myo-Inositol-3,6-diphosphate Ins(3,6)P2 Myo-Inositol-3,4,6-triphosphate Ins(3,4,6)P3
Myo-Inositol-1,3,4,5-tetraphosphate Ins(1,3,4,5)P4
Myo-Inositol-1,4,5,6-tetraphosphate Ins(1,4,5,6)P4
Myo-Inositol-1,2,3,4,6-pentaphosphate Ins(1,2,3,4,6)P5
PtdIns synthase
PtdIns Myo-Inositol Ins
PtdIns 4-kinase PTEN PtdIns 5-kinase
PtdIns-4,5-P2 PtdIns-4-P
PI3K
PtdIns-3,4,5-P3
PI3K PTEN
PLC
PLC PLC
PtdIns-3,4-P2
Ins-1,3,4,5-P4
Enoyl CoA hydratase
H2O
3-Hydroxypalmitoyl CoA
Phospho-myo-inositol Toolkit 2
Myo-Inositol-3-phosphate Ins(3)P1 Myo-Inositol-3,4-diphosphate Ins(3,4)P2 Myo-Inositol-3,6-diphosphate Ins(3,6)P2 Myo-Inositol-3,4,6-triphosphate Ins(3,4,6)P3
Thymidine kinase
ADP
5’-nucleotidase
5-Fluorouridine
5’-nucleotidase
5-FUMP
5-FdUMP
5-FUTP
5-FdUTP
RNA
DNA
Western Blotting
1. Resolve protein samples on SDS-PAGE 2. Electrophoretically transfer fractionated proteins from gel onto PVDF membrane 3. Block the membrane with neutral protein (BSA or milk casein)
4. Incubate the membrane with primary antibody specific to target protein
Sterol Toolkit
Cholesterol Pregnenolon Progesteron Aldosteron
Cortisol
Corticosterone
ATP FAD
Carnitine
3-Ketopalmitoyl CoA
FADH2
Palmitate
Acyl CoA dehydrogenase 3-Hydroxyacyl CoA dehydrogenase
NADH
Trans Δ2 enolopalmitoyl CoA
NAD
Cytoplasm
Intermembrane space
6. Incubate the blot with chemiluminescent HRP substrate and expose to film
5. Incubate the membrane with HRP-labeled secondary antibody specific to primary antibody
Flippase
ER
Dolichol
P
P
P
Mann-P-Dolichol transferases
P P Dolichol P Dolichol Glc-P-Dolichol transferases
P P Dolichol Dolicholpyrophofphatase Oligosacharyl transferase
Myo-Inositol-1,3,4,5-tetraphosphate Ins(1,3,4,5)P4
Thymidylate synthase
Biosynthesis and metabolism of phosphoinositides
Ins(3)P1 synthase
Myo-Inositol-3-phosphate Ins(3)P1 Glucose 6-pgosphate
Ins(3)P1 phosphatase Myo-inositol kinase
ATP
ADP + Pi Malonyl transacylase
Pantetheine
Acetyl transacylase
-ketoacyl ACP-synthase
Pantetheine
Fatty acid synthase
Enoyl-ACP reductase -ketoacyl ACP-reductase -hydroxyacyl ACP-dehydratase
Myo-Inositol-1,3,4-triphosphate Ins(1,3,4,)P3
Myo-Inositol-1,4,5-triphosphate Ins(1,4,5)P3
Myo-Inositol-3,4,5,6-tetraphosphate Ins(3,4,5,6)P4
Myo-Inositol-1,3,4,6-tetraphosphate Ins(1,3,4,6)P4
Androstendione
Testosteron
17-Estradiol
Estriol
1, 25-Dihydroxycholecalciferol
Cholic acid
Covalent modification of proteins
Poly(ADP ribosyl) Polymerase (PARP)
12-Deoxycholic acid
Biosynthesis of RNA
RNA Polymerase
Biosynthesis of DNA
DNA Polymerase
β-oxidation
Mitochondrial matrix
Palmitoyl carnitine
Citric Acid Cycle