《农业生物技术》试题三

姓名_____ 班级______ 分数______

一、选择题（每个2分，共30分）

1．植物组织培养中进行茎尖剥离的仪器设备是

A．显微镜B．放大镜 C．聚光镜D．解剖镜

2．下列用品中，不能采用干热灭菌法灭菌的是

A．棉塞B．剪刀C．培养皿D．解剖刀

3．下列激素中，不属于生长素的是

A．吲哚乙酸IAA B．萘乙酸NAA C．吲哚丁酸IBA D．玉米素ZT 4．大多数植物组织培养的温度为

A．15～20℃B．23～27℃C．25±2℃D．37±2℃5．采用70％～75%酒精对外植体进行消毒的时间一般为

A．3～5s B．0.2～2min C．3～5min D．5～10min

6、（）是植物组织培养最常用、最普及的无菌操作装置。

A.超净工作台

B.显微镜

C.培养箱

D.天平

7.外植体的灭菌常用( )。

A.干热灭菌

B.湿热灭菌

C.浸泡灭菌

D.擦拭灭菌

8、组织培养中，如超净工作台的局部环境消毒灭菌常用（）。

A.空气过滤灭菌

B.紫外线照射消毒

C.喷雾消毒

D.熏蒸消毒

9、熏蒸灭菌时，常用的熏蒸剂甲醛用量为（）。

A.0.1ml/m3

B.0.2ml/m3

C.1ml/m3

D.24ml/m3

10、玻璃器皿灭菌常用( )。

A. 75%酒精消毒

B.火焰灭菌

C.高温干热灭菌

D.高压蒸汽灭菌

11、植物组织培养中污染苗的灭菌常采用（）。

A．高温干热灭菌B．高压蒸汽灭菌C．紫外灯杀菌 D．70%酒精喷雾灭菌

12、下列对对胚的形成.芽的分化和幼苗转绿有促进作用的元素是（）。

A.铜

B.钼

C.硼

D.铁

13、在植物组织培养中，常用的维生素浓度约在（）mg/L之间。

A.0.1～0.2

B.0.1～1

C.10～100

D.50～100

14、下列不属于细胞分裂素的是（）。

A．2，4-D B．6-BA C．KT D．ZT

15、高温高压易被分解破坏的植物激素是( )。

A. IBA

B.GA

C.肌醇

D.维生素

16、在植物组织培养中，培养基的pH一般为（）。

A.低于5.0

B.5.6～6.5

C.6.0～7.0

D.7.0以上

17、适宜的外植体的大小是（）

A.0.2～05cm

B.0.5～1cm

C.1～1.5cm D1.5～2cm

18、下列植物器官哪种做外植体最合适（）。

A．茎尖.茎段B．叶片.叶柄 C．一年生枝条 D．幼嫩的茎尖.茎段

19、试管苗移植后，适宜的生根温度是（）。

A.10～15℃

B.15～18℃

C.18～20℃

D.20～25℃

20、超净工作台在每次接种前先用紫外线灯照射（）。

A.20min

B.40min

C.2h

D.20～30min

21、干热灭菌的温度应控制在( )，持续120min。

A. 100℃.

B. 120～150℃

C. 150～170℃

D. 121～125℃

22、温热灭菌主要用于( )灭菌，

A.培养基

B.赤霉素、玉米素、脱落酸等不耐热物质

23、组织培养中普遍采用的是对无菌操作的微环境进行( )灭菌。

A.空气过滤

B.紫外线

C.喷雾

D.熏蒸

24、组织培养中加温配制的培养基要趁热分装，低于（）℃易凝固。

A．25 B．30 C．50 D．60

25、固体培养基中琼脂的用量在( )之间。

A.3～5g/L

B.6～lOg/L

C.12g/L D． 20g/L

二、判断题（每个2分，共20分）

1、培养基经过高压灭菌后pH一般会升高0.2～0.3个单位。（）

2．铬酸洗液是强氧化剂，去污能力很强，可用来洗油脂多的器皿。（）3．从植物上分离真菌采样时最好选择阴天多云的日子。（）

4. 植物的任何器官包括细胞和组织都可作为外植体。（）

5．新玻璃器皿可以不用洗涤。（）6．杀死、消除或抑制部分微生物，使之不发生危害作用称为消毒。（）

7. 培养基中添加活性炭的作用是防止褐化；促进生根，增强琼脂的凝固能力。（）

8．已被霉菌等杂菌污染的器皿要用121℃高温高压蒸汽灭菌30min。（）9．用于进行琼脂、蔗糖和各种药品的称量，需要用分析天平。（）10．利用烘箱进行烘烤灭菌的方法，适用于各种玻璃器皿和器械的灭菌消毒。（）

三、名词解释（每个3分，共12分）

1、外植体

2、植物细胞的全能性

3、接种

4、灭菌

四、简答题（4小题，共28分）

1、简述植物组织培养特点？（4分）

2、简述植物组织培养快速繁殖的程序？（8分）

3、简述外植体的表面灭菌与接种程序？（8分）

4、阐述利用高压蒸气灭菌法进行培养基灭菌的具体操作步骤？（8分）

五综合题（10分）

按照所给配方MS+KT 1mg/L+IBA0.2mg/L，如何量取6L培养基的母液？

母液如下：大量元素20倍，铁盐100倍，有机物200倍，微量元素500倍，KT

0.5mg/mL，IBA 0.5mgmL。？

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