硝酸盐还原菌抑制硫酸盐还原菌

40–70
60
-
Desulfotomaculum
Up to 5
0.3-1.2
41–75
62
-
thermocisternum
Desulfovibrio gabonensis
1-17
5-6
15–40
30
-
Desulfovibrio longus
0-8
2
10–40
35
-
Lien and Bieder (1997)
+
norvegicus
Rozanova & Pivovarova (1988)
Beeder et al. (1995)
Michel Magot1,et.al.,Microbiology of petroleum reservoirs, Antonie van Leeuwenhoek 77: 103–116, 2000
Magot et al. (1992)
Desulfovibrio vietnamensis
0-10
5
12–45
37
-
Nga et al. (1996)
Thermodesulfobacterium
ND
ND
45–85
65
-
mobile
Thermodesulforhabdus
0-5.6
1.6
44–74
60
+ +
References
Beeder et al. (1994) Rees et al. (1995)
Desulfobacter vibrioformis
1-5
ND
Desulfobacterium cetonicum Up to 5
1
5–38
33
+
20–37
30-35
+
Desulfomicrobium
硝酸盐还原菌抑制硫酸盐还原 菌技术
佘跃惠 2010年3月
主要内容
• 内源菌概述； • 有益菌抑制有害菌； • 生物抑菌法机理； • 生物抑菌法在采油中应用
内源微生物概述
• 1.采油有益菌: • 烃氧化菌(HDB); • 硝酸盐还原菌(NRB); • 产甲烷菌.
• 2.有害菌: • 硫酸盐还原菌(SRB); • 铁细菌.
Nannobacteria as a by-product of enzyme-driven tissue decay-Ju¨rgen Schieber,et.al.Geology2009
砂岩表面生物膜 扫描电镜
EFFECTS OF SEM PREPARATION TECHNIQUES ON THE APPEARANCE OF BACTERIA AND BIOFILMS IN THE CARTER SANDSTONE---SARAH E. FRATESI, JOURNAL OF SEDIMENTARY RESEARCH, VOL. 74, NO. 6, NOVEMBER, 2004, P. 858–867
油藏中的SRB
• 从研究结果看出外源污染的或者土著的 SRB代表了油藏微生物中的一个重要的微 生物群落，它们可以跨越很宽的盐度，温 度同时在油藏中具有存在的普遍性。
FIG. Details from a fractured UUT stone from SEM analysis showing nanobacterium-like particles. The particles appeared as coccoid
D:纳米菌 Close-up of granular-textured area shows it to consist of spheroidal bodies
. ranging in size from 40 to 80 nm. These are nannoballs
Nannobacteria as a by-product of enzyme-driven tissue decay, Ju¨rgen Schieber et.al. Geology2009
Figure. Comparison of nannobacterial textures. At left, picture is from nannobacteria， At right, granular nannoball
texture in decaying squid muscle (nannoballs range in size from 50 to 120 nm).
Galushko & Rozanova (1991)
Rozanova et al. (1988)
Tardy-Jacquenod et al. (1998)
Nazina et al. (1988)
Nazina & Rozanova (1978)
Nilsen et al. (1996b)
Tardy-Jacquenod et al. (1996b)
structures grouped together in coarse clusters. Their diameter was between 200 and 300 nm. Bar, 1 m. Magnification, 15,000.
Michel Drancourt, et.al. J. CLIN. MICROBIOL, 2003
0-8
ND
apsheronum
‘Desulfotomaculum
1-14
4-6
halophilum’a
Desulfotomaculum kuznetsovii 0-3
0
Desulfotomaculum nigrificans Up to 4
1
4–40
25-30
-
30–40
35
-
50–85
60-65
+
Species
Archaeoglobus fulgidus D源自文库sulfacinum infernum
Salinity (%) Range 0.02-3 0-5
Optimum 2 1
Temperature Range 60–85 40–65
Optimum 76 60
Complete oxidizer
油藏环境中的内源微生物
2.硫酸盐还原菌（SRB)
• 硫酸盐还原菌 由于其危害性（ SRB每年腐 蚀结垢给油田造成的损失达数百亿元）， 油田产出水中的SRB已经被广泛研究。
• 存在于地表设备腐蚀的常温SRB和主要导 致油藏酸化堵塞结垢的嗜热SRB存在明显 差异。
表1 从油田分离的常温和嗜热硫酸盐还原菌