上机操作一 RPHPLC测定US EPA 规定的16种优先监测多环芳烃结果分析

上机操作一RPHPLC测定US EPA 规定的16种优先监测多环芳烃结果分析用RPHPLC对USA EPA 规定的16种优先监测多环芳烃10次测定的数据如下表，请用Excel

软件列出计算公式并计算16种多环芳烃峰高和峰面积的均值、标准差、变异系数和检测限（3σ）；

根据上述结果说明测定时用峰高还是用峰面积好？

Name Conc.,

Peak height, mAu

μg/ml

Naphthalene 0.4 0.80.8 0.85 0.85 0.76 0.84 0.8 0.8 0.84 0.86 Acenaphthylene 0.8 1.1 1.15 1.2 1.1 1.15 1.18 1.18 1.13 1.13 1.2 Acenaphthene 0.4 0.30.30.30.280.30.30.30.380.30.3 Fluorene 0.08 0.80.80.70.80.80.840.80.80.840.8 Phenanthrene 0.04 0.95 1.2 1.1 1.2 1.15 1.2 1.18 1.18 1.18 1.2 Anthracene 0.04 2.9 2.7 2.65 2.7 2.7 2.7 2.73 2.73 2.73 2.82 Fluoranthene 0.08 0.60.764 0.60.70.650.650.65 0.65 0.6 0.68 Pyrene 0.04 0.250.371 0.250.280.30.30.3 0.27 0.3 0.23 Benzo[a]anthracene 0.04 0.9 1.071 1.072 1.099 1.08 1.113 1.109 1.117 1.1 0.8 Chrysene 0.04 1.4 1.731 1.716 1.779 1.719 1.82 1.844 1.841 1.79 1.64 Benzo[b]fluoranthene 0.08 1.839 1.948 1.895 1.931 1.838 1.885 1.854 1.886 1.811 1.856 Benzo[k]fluoranthene 0.04 0.5070.45 0.5490.450.450.450.45 0.5 0.45 0.41 Benzo[a]pyrene 0.04 0.6410.645 0.60.8390.680.560.56 0.6 0.55 0.57 Dibenzo[a,h]anthracene 0.08 0.4150.45 0.450.450.430.350.35 0.38 0.35 0.35 Benzo[g,h,i]perylene 0.08 0.1130.1 0.150.150.120.150.15 0.15 0.15 0.12 Indeno[1,2,3-cd]pyrene 0.04 0.2870.3 0.30.30.280.30.3 0.3 0.3 0.3

Name Conc.,

Peak area, mAu

μg/ml

Naphthalene 0.4 43.91346.49955.13738.0137.9437.92837.09937.25337.37537.063 Acenaphthylene 0.8 62.61557.667.00246.70749.35548.96550.18850.78149.42647.716 Acenaphthene 0.4 27.24531.36524.20922.90918.59318.87817.23517.99524.78515.249 Fluorene 0.08 49.91423.39431.3729.04135.07837.03836.48135.9236.12720.27 Phenanthrene 0.04 67.22935.822 41.34238.40540.21934.46239.96842.05741.13230.582 Anthracene 0.04 88.07134.984 47.26644.26246.44546.58947.03347.763 44.247 46.951 Fluoranthene 0.08 18.774 5.18 7.4057.1467.2459.6958.057 8.301 6.821 6.527 Pyrene 0.04 2.988 2.028 5.173 3.116 3.494 3.366 3.41 3.314 4.184 3.358 Benzo[a]anthracene 0.04 8.04 5.008 5.225 5.394 6.5297.296 6.803 7.109 6.208 7.22 Chrysene 0.04 11.6749.064 10.91211.219.8710.0729.998 10.102 9.804 10.144 Benzo[b]fluoranthene 0.08 9.3689.733 9.4949.7059.2999.6819.467 9.709 9.29 10.985 Benzo[k]fluoranthene 0.04 10.46910.896 10.79212.81310.05910.83813.077 10.322 10.032 15.911 Benzo[a]pyrene 0.04 12.48319.195 19.48421.89518.02412.53220.122 17.95 17.48 17.901 Dibenzo[a,h]anthracene 0.08 4.21421.291 23.35924.35423.148 6.03425.208 22.374 21.583 16.941 Benzo[g,h,i]perylene 0.08 0.9488.689 9.059.1398.579 1.51810.039 9.218 9.496 6.562 Indeno[1,2,3-cd]pyrene 0.04 2.71725.717 26.61427.65327.0748.2929.043 28.419 27.161 20.999