真核生物转录机理与调控
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Holoenzyme: Core enzyme (β’, β & α) + Subunit σ RNA pol II: Core subunits (1, 2 & 3) + Subunits 4-12
RNA polymerase II
蟹钳
RNA polymerase III
RNA precursor
4.1 Eukaryotic RNA polymerases
T表ab4le.15.1 Eukaryotic RNA polymerases and their roles
表 4表.15.1
真核生物 RNA 聚合酶 和它们的作用
Polymerase Location
Products
Polymerase I N核u仁cleolus Polymerase II N核u基cle质oplasm Polymerase III N核u基cle质oplasm
Molecular Biology Chaper 4
Transcription in Eukaryotes: Mechanism and Regulation
Content
4.1 Eukaryotic RNA polymerases 4.2 Eukaryotic Promoters 4.3 General Transcription Factors and Initiation 4.4 Specific Transcription Factors and Transcriptional Regulation 4.5 Structures of specific transcription factors 4.6 Experiments
RNA polymerase III Processing
4.5S tRNA
5S
U6
rRNA snRNA
Three types of RNA polymerase and its transcript in Eukaryotes
Transcription in Eukaryotes
Transcripition
TATA box + – –
DPE: Downstream Promoter Element
G
A T
CG
Initiator / 起始子 PyPyAN TAPyPy
drive basic transcription 驱动基础转录
BRE: TFIIB Recognition Element
GGG CCA
my promoter. What would you like?”
“Well, I prefer BRE and DPE.”
TATA box
-10 box TATAAT
TATAAA
TATA框最常出现在特殊的基因里
Specialized genes Housekeeping genes Genes of development
Prokaryotic promoter
Eukaryotic promoter
4.2 Eukaryotic Promoters
TFIIB识别元件 TATA框
起始子
上游启动子 元件
Biblioteka Baidu
下游启动子 元件
Eukaryotic promoter
“I would like to have TATA box and Initiator as
原核生物 RNA 聚合酶
4.3.1 TFIID
Transcripition of mRNA and
Transcripition of tRNA and
of rRNA gene snRNA genes 5S rRNA gene
RNA Pol I RNA Pol II RNA Pol III
mRNA
4.2 Eukayotic Promoters
4.2 Eukayotic Promoters
CGCC
4.3 General Transcription Factors and Initiation
真核生物 RNA polymerase II: “I need help from other proteins.”
IIB
IIF
IIA IID
IIE IIH
TATA
RNA pol II
box
前起始复合体
Locations of RNA polymerase Nucleolus ( RNA polymerase I )
Nucleoplasm ( RNA polymerase II and III )
RNA polymerase I
rRNA precursor
RNA polymerase I Processing
Transcription in Eukaryotes: Mechanism and Regulation
Specific transcription
factor
General transcriptio
n factors
General transcription
factors
Eukaryotic RNA polymerase II and transcription factors
Transcription in Eukaryotes: Mechanism and Regulation
Prokaryote
Eukaryote
Transcription in Eukaryotes: Mechanism and Regulation
Transcription in prokaryotes
5.8S rRNA
18S rRNA
28S rRNA
RNA polymerase II
mRNA
RNA polymerase II Translation
RNA precursor Processing
Protein
snRNAs
RNA polymerase II
Rpb1、Rpb2和 Rpb3称为‘核心亚基’
A large rRNA precursor for 5.8S, 18S, and 28S rRNAs
hnRNAs (precursors for mRNAs) and most of the snRNAs
Precursors for 5S rRNA and 4.5S tRNAs, and U6 snRNA, etc.
RNA polymerase II
蟹钳
RNA polymerase III
RNA precursor
4.1 Eukaryotic RNA polymerases
T表ab4le.15.1 Eukaryotic RNA polymerases and their roles
表 4表.15.1
真核生物 RNA 聚合酶 和它们的作用
Polymerase Location
Products
Polymerase I N核u仁cleolus Polymerase II N核u基cle质oplasm Polymerase III N核u基cle质oplasm
Molecular Biology Chaper 4
Transcription in Eukaryotes: Mechanism and Regulation
Content
4.1 Eukaryotic RNA polymerases 4.2 Eukaryotic Promoters 4.3 General Transcription Factors and Initiation 4.4 Specific Transcription Factors and Transcriptional Regulation 4.5 Structures of specific transcription factors 4.6 Experiments
RNA polymerase III Processing
4.5S tRNA
5S
U6
rRNA snRNA
Three types of RNA polymerase and its transcript in Eukaryotes
Transcription in Eukaryotes
Transcripition
TATA box + – –
DPE: Downstream Promoter Element
G
A T
CG
Initiator / 起始子 PyPyAN TAPyPy
drive basic transcription 驱动基础转录
BRE: TFIIB Recognition Element
GGG CCA
my promoter. What would you like?”
“Well, I prefer BRE and DPE.”
TATA box
-10 box TATAAT
TATAAA
TATA框最常出现在特殊的基因里
Specialized genes Housekeeping genes Genes of development
Prokaryotic promoter
Eukaryotic promoter
4.2 Eukaryotic Promoters
TFIIB识别元件 TATA框
起始子
上游启动子 元件
Biblioteka Baidu
下游启动子 元件
Eukaryotic promoter
“I would like to have TATA box and Initiator as
原核生物 RNA 聚合酶
4.3.1 TFIID
Transcripition of mRNA and
Transcripition of tRNA and
of rRNA gene snRNA genes 5S rRNA gene
RNA Pol I RNA Pol II RNA Pol III
mRNA
4.2 Eukayotic Promoters
4.2 Eukayotic Promoters
CGCC
4.3 General Transcription Factors and Initiation
真核生物 RNA polymerase II: “I need help from other proteins.”
IIB
IIF
IIA IID
IIE IIH
TATA
RNA pol II
box
前起始复合体
Locations of RNA polymerase Nucleolus ( RNA polymerase I )
Nucleoplasm ( RNA polymerase II and III )
RNA polymerase I
rRNA precursor
RNA polymerase I Processing
Transcription in Eukaryotes: Mechanism and Regulation
Specific transcription
factor
General transcriptio
n factors
General transcription
factors
Eukaryotic RNA polymerase II and transcription factors
Transcription in Eukaryotes: Mechanism and Regulation
Prokaryote
Eukaryote
Transcription in Eukaryotes: Mechanism and Regulation
Transcription in prokaryotes
5.8S rRNA
18S rRNA
28S rRNA
RNA polymerase II
mRNA
RNA polymerase II Translation
RNA precursor Processing
Protein
snRNAs
RNA polymerase II
Rpb1、Rpb2和 Rpb3称为‘核心亚基’
A large rRNA precursor for 5.8S, 18S, and 28S rRNAs
hnRNAs (precursors for mRNAs) and most of the snRNAs
Precursors for 5S rRNA and 4.5S tRNAs, and U6 snRNA, etc.