百合育种

2n-gametes have been successfully used for
producing sexual polyploids. An advantage of 2ngametes is that the F1 hybrids can directly be used for further crossing. When introgression of specific traits is the aim, the use of 2n-gametes and sexual polyploidization is preferable as compared to mitotic chromosome doubling
We studied the extent of intergenomic
recombination in two different types of polyploid progenies derived from two interspecific Lilium hybrids Diploid hybrids L. longiflorum ´ L. rubellum (LR) and L. longiflorum ´ Asiatic hybrid (LA).
Homoeologous Recombination in Interspecific Hybrids of Lilium
百合属植物种间杂交
的部分同源重组
基因重组是指生物体进行有性生殖的过程中，控制
不同性状的基因的重新组合。 异源多倍体：生物学名词，指不同物种杂交产生的 杂种后代经过染色体加倍形成的多倍体。 性状分离：指在后代中同时出现显现性状和隐性性 状。 基因分离定律的实质: 位于一对同源染色体上的等位基因,具有一定的独立 性,生物体在进行减数分裂形成配子时,等位基因会 随着同源染色体的分开而分离,分别进入到两个配子 中,独立地随配子遗传给后代.
INTRODUCTION
The genus Lilium has been classified into
seven sections with more than 80 species Plant height, disease resistance, and flower color and size.（价值） Interspecific hybridization is inevitable sterile due to disturbed chromosome pairing during meiosis
Two different types of polyploid progenies
were analyzed by genomic in situ hybridization (GISH) in order to estimate the extent of homoeologous recombination。 ALA-triploids and AALA-tetraploids showed considerable intergenomic recombination explainable by homoeologous pairing in LAhybrid during the origin of 2n-gametes。
BC1 progenies Meiosis and pollen fertility in LR and LA hybrids
F1 (LA and LR) showed intermediate
phenotypic characters of the parents BC1 progenies (ALA) showed genetic segregation All LLR-hybrids (BC） showed highly homogeneous and intermediate
‘Montreux’, ‘Puccini’ and ‘Meribel’, and accession number ‘78251’ were used as female parents grown in a greenhouse Day (17-20℃) Night (14-18 ℃)
Chromosome preparation
L. rubellum (RR)
Asiatic hybrid ‘Whilito’ (AA)
wenku.baidu.com LL ×RR
LL×AA ↓ ↓ LR(F1)× ‘Snow Queen’, LA (F1) ↓ LLR(2n=3x=36) LLRR ×OO LA×AA LA ×OO ↓ ↓ ↓
OLR
ALA
OLA
diploid cultivars of Asiatic hybrids, viz.,
frequencies of bivalents and univalents
varied in different pollen mother cells. Pollen of LR-hybrids was highly sterile. first division restitution (FDR) gametes containing 24 chromatids
We have used triploids successfully in
crossing with both diploid and tetraploid genotypes and produced large numbers of aneuploid progenies (unpublished data).
MATERIALS AND METHODS
Plant materials
Chromosome preparation DNA isolation and probe preparation
In situ hybridization
Plant materials
Lilium longiflorum, c.v. ‘Gelria’ (LL)
DISCUSSION
sexual polyploidization is the most desirable
approach to lily Colchicine increase fertility(育性) sexual polyploidization is equally important to allopolyploids， intergenomic recombination occurs during formation of 2n-gametes in distantly relatedF1 hybrids
Roots were kept overnight in saturated a-
bromonaphtalene solution at 4℃ and fixed in ethanol acetic acid (3:1) for at least 2 h.
RESULTS(结果)
Morphological observation of the F1 and