(综述)植物化学成分及其代谢物的定性和定量分析

氧杂蒽酮的化学结构（1-15）确定
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Fig. 2. UHPLC–MS chromatograms of the standard xanthones and the crude extracts of G. hanburyi. (A) UHPLC chromatograms of 12 standard xanthones, (B) TIC of the 12 standard xanthones, (C) UHPLC chromatograms of the crude extracts of G. hanburyi, (D) TIC of the crude extracts of G. hanburyi.
Reproduced with permission from [89]. Figure options
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Fig. 5. HSCCC chromatogram of gamboge extract (190 mg). Fraction I (17.26 mg), fr. II (5.48 mg), fr. III (3.81 mg), fr. IV (3.56 mg), fr. V (7.66 mg), fr. VI (3.21 mg), fr. VII (8.21 mg), fr. VIII (35.25 mg), fr. IX (76.78 mg), fr. X (6.04 mg), fr. XI (4.34 mg).
Medical College, Beijing 100193, PR China
b
Department of DMPK, AstraZeneca Pharmaceuticals, 35 Gatehouse Drive, Waltham, MA 02451, USA Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100026, PR China Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, PR China
。2。UHPLC-MS 色谱图的 标准呫吨酮和 G. hanburyi 粗提物中。 （A）（B）UHPLC 色谱图的 12 个标准 氧杂蒽酮，TIC 的 12 个标准氧杂蒽酮（C）UHPLC 色谱 G. hanburyi 粗提物中， （D）议会的粗提物的 G. hanburyi。
Reproduced with permission from [89]. Figure options
Reproduced with permission from [89]. Figure options
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Fig. 4. Proposed fragment pathways of [M + H]+ for: (A) isogambogenin (13), (B) isomorellinol (14), (C) 15.
Haifeng Wua, Jian Guob, Shilin Chena, Xin Liuc, Yan Zhoud, ,Zhanga, Xudong Xua,

a
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union
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Fig. 3. (A) MS/MS spectra of isogambogenin (13) of selected [M + H]+ at m/z 615, (B) MS/MS/MS spectra of selected ion at m/z 491 from m/z 615, (C) MS/MS spectra of isomorellinol (14) of selected [M + H]+ at m/z 547, (D) MS/MS/MS spectra of selected ion at m/z 461 from m/z 547, (E) MS/MS spectra of compound 15 of selected [M + H]+ at m/z 545, (F) MS/MS/MS spectra of selected ion at m/z 461 from m/z 545 (collision energy at 30 eV).
Figures and tables from this article:
Fig. 1. Chemical structures of caged xanthones (1–15) identified from G. hanburyi.
Reproduced with permission from [89].
Reproduced with permission from [92].
图。 已提议片段途径的[M + H]+为： （A） （13） ， （B） 4。 isogambogenin isomorellinol （14），（C）15。 转载许可[89]。
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图。3。（A）MS /，MS 谱 isogambogenin（13）选定的[M+ H] + m / z 为 615， （B）选择离子从 m / z615，m / z 为 491 MS / MS / MS 谱（C）MS / MS，光 谱 isomorellinol（14）选择[M+ H] + m / z 为 547（D）MS / MS / MS 谱的选择 离子为 m / z461 M / Z547，（E）MS/ MS 谱的化合物 15，所选的[M + H]+为 m/ z545（F）MS / MS/ MS 谱图，从 m / z545（碰撞能量 30 eV 的选择离子为 m / z461）。
Keywords

Liquid chromatography; Mass spectrometry; Medicinal plants;

Traditional Chinese medicine; Fingerprinting
抽象 在过去的几年中，液相色谱与质谱联用（LC-MS）在天然产物分析的应用都得到了显着增 长，因为日益提高的分离和检测能力的 LC-MS 仪器。特别是，新颖的高分辨率混合工具与 其他分离或分析技术链接到超高性能 LC 的 LC-MS 和断字大大有助于在复杂基质中的微量 浓度的明确识别和天然产品的高度敏感的定量。提供最新的植物性化合物的分析概述 LC-MS 应用的目的，论文发表在最近几年（2007-2012 年） ，涉及的植物化学成分及其代谢 物的定性和定量分析总结在本次审查中。 在简要说明天然产物的分析， 最显着的特点 LC-MS 和样品制备技术的一般特点，本论文主要集中在酚类物质（包括类黄酮） ，生物碱，萜类化 合物，类固醇，香豆素，木脂素类化合物的筛选和鉴定，和杂项在各自的草药化合物和生物 样品，以及中国传统医学（中医）处方使用串联质谱仪。化学指纹分析用 LC-MS 也被描述。 同时，仪器的特点和方法的细节突出。
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/10.1016/j.jpba.2012.09.004, How to Cite or Link Using DOI

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Abstract
Over the past few years, the applications of liquid chromatography coupled with mass spectrometry (LC–MS) in natural product analysis have been dramatically growing because of the increasingly improved separation and detection capabilities of LC –MS instruments. In particular, novel high-resolution hybrid instruments linked to ultra-high-performance LC and the hyphenations of LC–MS with other separation or analytical techniques greatly aid unequivocal identification and highly sensitive quantification of natural products at trace concentrations in complex matrices. With the aim of providing an up-to-date overview of LC–MS applications on the analysis of plant-derived compounds, papers published within the latest years (2007–2012) involving qualitative and quantitative analysis of phytochemical constituents and their metabolites are summarized in the present review. After briefly describing the general characteristics of natural products analysis, the most remarkable features of LC–MS and sample preparation techniques, the present paper mainly focuses on screening and characterization of phenols (including flavonoids), alkaloids, terpenoids, steroids, coumarins, lignans, and miscellaneous compounds in respective herbs and biological samples, as well as traditional Chinese medicine (TCM) prescriptions using tandem mass spectrometer. Chemical fingerprinting analysis using LC–MS is also described. Meanwhile, instrumental peculiarities and methodological details are accentuated.
Fig. 6. Scheme of the HPLC on-line coupled to ESI-MS and DPPH assay. The arrows indicate flow directions.
在简要说明天然产物的分析最显着的特点lcms和样品制备技术的一般特点本论文主要集中在酚类物质包括类黄酮生物碱萜类化合物类固醇香豆素木脂素类化合物的筛选和鉴定和杂项在各自的草药化合物和生物样品以及中国传统医学中医处方使用串联质谱仪
Recent developments in qualitative and quantitative analysis of phytochemical constituents and their metabolites using liquid chromatography–mass spectrometry