908737 - Post Closing in Material Ledger CKMLCP 基本业务逻辑
UL8750最新英文版
UL 8750Light Emitting Diode (LED) Equipment for Use in Lighting ProductsMA Y 22, 2014 − UL 8750tr1UL Standard for Safety for Light Emitting Diode (LED) Equipment for Use in Lighting Products, UL 8750UL标准安全发光二极管(LED)设备用于照明产品、UL 8750First Edition, Dated November 18, 2009第一个版本,日期为2009年11月18日Summary of Topics 总结的主题This revision to ANSI/UL 8750 includes the following changes in requirements:这个修订ANSI / UL 8750变化包括以下要求:●Clarify requirements for conformal coatings, paragraph 7.7.27.7.2澄清要求保形涂料、段落●Insulation materials in transformers and coils ± Delete paragraph 7.11.2.11 and add Section 7.11.3绝缘材料在变形金刚和线圈±删除段落7.11.2.11 7.11.3并添加部分●Revise Risk of Fire De®nition to include 15 W power limit and revisions to Class 2 and LVLE referencesthroughout the standard修改火的风险包括15 W功率极限和修正二班和LVLE引用标准●Add requirements for supply and load connections添加需求供应和负载连接●Revisions to consolidate electrical spacings in Sections 7.7 and 7.8 and add optional shorting test forclosely-spaced PWB traces修订整合电气间距在章节7.7和7.8和添加可选做空测试间隔太近PWB痕迹●Add requirement for LED array (module) thermal measurement point添加要求LED阵列(模块)热计量点●Add temperature measurement method for polymeric materials when TC is optically radiated添加温度测量方法,当TC光学辐射高分子材料Text that has been changed in any manner or impacted by UL's electronic publishing system is marked with a vertical line in the margin. Changes in requirements are marked with a vertical line in the margin and are followed by an effective date note indicating the date of publication or the date on which the changed requirement becomes effective.文本已经以任何方式改变或影响UL电子出版系统的边缘有一条垂直线。
造纸专业常用英文缩略语
造纸专业常用英文缩略语AAA = atomic absorption原子吸收ABS = acrylonitrile-buladrene styrene丙烯腈—丁;烯—苯乙烯ACAR = angular correlation of annihilation radiation消除辐射的角相关性AM = acrylamide丙烯酰胺AOX = adsorbable organic halides可吸附的有机卤化物AP = plkali pulp碱法纸浆APAM = anionic polyacrylamide阴离子型聚丙烯酰胺ASB = aerotion stabilization basin稳定曝气池AST = activated sludge treatment活性污泥处理BBCT = best convential pollutant cotrol technology最常用污染物控制技术BDMT = bone dry metric tons绝干公吨BME = bipolar membrane electro dialysis两极膜电透析BMP = best management practices最优管理实践BOD = biochemical oxygen demand生化耗氧量BP = boiling point沸点BPK = bleached papergrade kraft and soda(生产)白纸用硫酸盐和荷性纳法浆BPT = best practicable control technology最佳实用控制技术BTU = british thermal unit英热单位BW = basis weight定量CCAD = computer aided design计算机辅助设计CBLI = chemistry-based leak indicator化学(法)示漏器CC = consistency controller浓度调节器CFD = computational fluid dynamics计算流体动力学CI = colour index比色指数= cofidence interval置信区间CL = colored ledger彩色底板CLSM = confocal laser scanning microscopy共焦激光扫描显微镜CMC = carboxy methylated cellulose羧甲基纤维素COMS = compliance optimization modeling system寻优模型系统CP = chemical pulp化学浆= chemical pure化学纯CPPC = coordinated phosphate/pH chemistry controller配位磷酸盐/pH 调节器CR = consistency regulator浓度调节器CRP = chloride removal process氯化物排出法CSD = condensate steam distillation column冷凝汽馏塔CTMP = chemical treatment in terms of sulphonation硫化期间的化学处理= chemithermomechanical pulp化学热磨机械浆CTU = centigrade thermal unit公制热量单位CV = coefficient variation偏离系数= crystal violet结晶紫DD = dioxide二氧化物DAF = dissolved air floatation(溶)气浮DCS = dissolved and colloidal substances溶解与胶态物= distributed control system集散控制系统DELS = Doppler electrophoretic light scattering多普勒电泳光扫描DIP = deinked pulp脱墨纸浆DKP = deinked kraft pulp脱墨牛皮纸浆DLK = double-line clippings双线限位DMS = dynamic mechanical spectroscopy动力谱学DMSO = dimethyl sulfoxide二甲亚砜DMT = dimethyl terephthalate对邻苯二甲酸二甲酯DO = dissolved oxygen溶解氧DP = degree of polymerization聚合度DSC = differential scanning calorimetry微分扫描量热法DVC = digital valve controller数字伐控制器EEC = embedded costs插入成本ECF = elemental chlorine free无元素氯(漂白)EDTA = ethylene eiamine tetraacetic acid乙二胺四乙酸EPC = experimental prismatic calcite实验棱镜方解石ERV = estimated replacement value预计取代值ESP = electrostatic precipitator静电滤尘器= emergency shutdown procedure事故停机程序EVA = ethylene vinyl acetate乙烯乙酸乙烯酯ESPRA = empire state paper research associates国立造纸研究会EVOH = ethylene-vinyl alcohol乙烯-乙烯醇FFAS = formamidine sulfinic acid甲脒亚磺酸FBB = folding box board折叠箱纸板FBK = fully bleached kraft全漂牛皮纸FC = flow controller流量控制器FID = free induction decays自由感应衰减FP = freezing point冰点;凝固点GGDP = gross domestic product本国生产总值GEMS = general energy and materials balance system通用能量和物料平衡系统GLC = gas-liquid chromatography气液色谱GPC = gel permeation chromatographic analysis凝胶渗透色谱分析GPM = gallons per minute加仑/分钟HHC = high consistency高浓HCR = high consistency refiner高浓磨浆机HD = high density高密度HPR = high production rate高生产率HPSEC = high-performance size-exclusion chromatography高性能粒度筛析色谱法HRT = hydraulic retention time水力停留时间HTH = high test hypochlorite高级漂粉HV = high voltage高压HW = hardwood硬木IIMPM = interactive multiplanar model相互作用的多面模型IPST = institute of paper science and technology造纸科技研究院IWC = international water consultants国际水质顾问团JJIT = just-in-time正好;准时KKP = kraft pulp牛皮浆;硫酸盐浆LLC = level controller液面控制器LCC = lignin-carbohydrate complexes木素-碳水化合物复合体LCL = lower control limits控制下限LCR = level cotroller and recorder液面控制记录仪LDPE = low density poly ethylene低密度聚乙烯LDV = laser Doppler velocimetry激光多普勒测速法LIVG = low inlet velocity gasification process低入口速度气化工艺LPR = low production rate低生产率LRD = long rang dependence广范围相关LVDT = linear position transducer线性位移变送器LWC = lightweight coated低定量涂布的MMACT = maximum achievable control technology最大可达控制技术MAP = modified atmosphere packaging改良常压包装法MC = marginal cost边际成本= medium consistency中浓(度)MDI = methylendiphenyl diisocyanate亚甲苯二苯二异氰酸酯MeB = methylene blue亚甲基兰,四甲基兰MEK = methyl ethyl ketone甲(基)乙(基)酮MF = machine finished机械整饰的MG = machine glazed机械上光的= malachte green孔雀绿MISS = mixed liquor suspended solids (有机物与活性污泥 )混合液中悬浮固体MOW = mixed office waste混合办公废纸MRP = matal removal process金属(离子)脱除过程MSW = municipal solid waste城市固体废物MVP = moisture vapor permeability水蒸汽渗透性MWL = milled wood lignin磨木木素NNC = nitrocellulose 硝化纤维素NF = nanofiltration超滤 (毫微过滤)NMR = nuclear magnetic resonance核磁共振NSPS = new source performance standards新的资源性能标准NSSC = neutral sulfite semi-chemical pulp中性亚硫酸半化学浆OOCC = old corrugated container旧瓦楞纸箱OD = over dry绝干;烘干OEE = overall equipment efficiency总设备效率OIT = oxidative induction temperature氧化起始温度O&M = operating and maintenance 使用与维护ONP = old newspaper旧新闻纸OPP = oriented polypropylene取向聚丙烯OPR = oil penetration rates渗油率OWL = oxidized white liquor氧化白液PPAL = positron annihilation life time正电子湮没寿命PC = pressure controller压力调节器PCA = principal components analysis主成分分析PCC = precipitated calcium carbonate沉淀碳酸钙PCR = pressure controller and recorder压力调节记录仪PDSC = pressure differential scanning colorimetry压差扫描量热术PEMS = predictive emissions modeling system预测排放模型系统PEO = poly ethylene oxide聚氧化乙烯PGS = papergrade sulfite造纸用硫磺PGW = pressurized groundwood压力磨木浆PM = paper machine 造纸机;抄纸机PM/ECCM = preventive maintenance and essential care and condition monitoring预防维修/基本维修及状态监测PP = polypropylene聚丙烯PSES = pretreatment standards for existing sources现存资源预测标准PSM = process safety management(生产)过程安全管理PTFE = polytetrafluoroethylene聚四氟乙烯PTR = photothermal radiometry光热辐射分析法PVC = polyvinylchloride聚氯乙烯PVDC = polyvinyl dichloride聚二氯乙烯PVSK = polyvinylsulfate聚乙烯硫酸酯RRDH = rapid displacement heating快速置换加热法RH = relative humidity相对湿度RMP = refiner mechanical pulp木片磨木浆;盘磨机械浆RN = regular number纸板标准号RT = radiographic testing射线照相试验,X射线检验SSBK = solid bleached kraft(同质)漂白牛皮纸SBR = sequencing batch reactors程序化间歇反应器SC = super calendered超级压光的SDI = silt density index淤泥浓度指数SE = supplemental energy补充能量;辅助能SEC = size exclusion chromatographic粒度筛析色谱法SEM = scanning electron microscope扫描电子显微镜SEM-EDS = scanning electron microscope-energy dispersive spectrometry扫描电子显微镜—能量分散能谱测定法SGW = stone ground wood磨石磨木浆SIF = stress intensity factor应力强度系数;应力强化因子SOPs = standard operating procedures标准作业程序SP = sulphite pulp亚硫酸盐纸浆SPC = satislical process control过程控制SRT = solids retention time粒子留着时间SUB = solid unbleached board(同质)本色浆纸板SW = softwood软木;针叶树SWL = sulphite waste liguor亚硫酸盐废液TTAC = totally applied chlorine总用氯量TC = temperature controller温度调节器TCDF = tetrachlorodibenzofuran四氯二苯并呋喃TCF = totally chlorine-free全无氯(漂白)TCR = temperature controller and recorder温度调节记录仪TGA = thermal gravimetric analysis热重分析TLA = thin layer activation薄层活性化TMP = thermo mechanical pulp热磨机械浆TP = thermo-plastic热塑性的TQ = threshold quantity临界量(值)TRS = total reduced sulfur总还原硫TS = tensile strength抗张强度TSS = total suspended solids总悬浮固体量UUBB = unbeached board本色(浆)纸板UBK = unbeached kraft本色牛皮纸UCL = upper control limits控制上限UT = ultrasonic testing超声试验UV = ultraviolet紫外光VVOC = volatile organic compound挥发性有机化合物WWAS = waste-activated sludge废活性污泥WFMT = wet fluorescent magnetic particle test湿荧光磁粉试验WL = white ledger白色帐簿纸WLC = white-lined chipboard白浆衬里的粗纸板WP = wood pulp木浆WVTR = water vapor transmission rate水蒸汽传递速度YYI = yellow index返黄值;返黄指数YP = yield point屈服(软化)点。
Diodes Inc. Material Data Sheet.pdf_1693271463.264
Diodes Inc. Material Data SheetRev: Aug 2011Part Number:B0530W-7-F, B0540W-7-F, B0520LW-7-F Weight (mg):10.655Element Material Group Materials CAS (ifapplicable)Average mass homogeneous Materal(%)Percent ofwhole (%)Mass (mg)ppmHomogeneous Materialppm overallDie,SchottkyDoped siliconDoped silicon *7440-21-3100.00% 3.4470.367100000034469 Fe 7439-89-657.65%576500162062 Ni 7440-02-041.00%410000115256 Mn7439-96-50.60%60001687Cr(not Cr 6+)7440-47-30.10%1000281 Co 7440-48-40.50%50001406 Si 7440-21-30.15%1500422Pure silver Ag 7440-22-4100.00%0.853 0.091 10000008530 Bonding wire1.4milAu 7440-57-5100.00%0.512 0.055 10000005122SiO260676-86-069.00%690000 441884 Epoxy Resin 29690-82-214.00%140000 89658 Phenol Resin 9003-35-47.00%70000 44829Mg(OH)21309-42-88.00%80000 51233 C 1333-86-40.20%2000 1281 others ---- 1.80%18000 11527 Ag7440-22-480.00%8000008256Bisphenol F28064-14-415.00%1500001548Glycidyl neodeconate26761-45-5 5.00%50000516Tin solder Pure Tin Sn7440-31-5100.00%2.003 0.213 100000020035 100.0010.6551000000Tolerance±10%Antimony compounds Organic tin compoundsAsbestos Ozone Depleting Substances - Class I (CFCs, HBFCs, etc.)Azo compoundsOzone Depleting Substances - Class II (HCFCs)Cadmium and cadmium compounds Perfluorooctane Sulphonate (PFOS) or related compoundsCertain Shortchain Chlorinated Paraffins Polybrominated biphenyls (PBB) and Polybrominated diphenyl ethers (PBDE) including D ecaBDE Chlorinated organic compounds Polychlorinated Biphenyls (PCBs)HalogensPolychlorinated Naphthalenes ( > 3 chlorine atoms)Hexavalent chromium compounds Radioactive SubstancesLead and lead compounds Tributyl Tin (TBT) and Triphenyl Tin (TPT)Mercury and mercury compoundsTributyl Tin Oxide (TBTO)* The Silicon Chip is doped at atomic levels with trace amounts of elements that may include Phosphorus, Boron, Arsenic, and other elements. Metalization mayinclude Titanium, Nickel, Aluminum, Silver or Gold These substances are not reported where their concentration is less than the minimum reportable level per the guidelines specified in the Tables of EIA JIG-101, Material Composition Declaration for Electronic Products.This product or product family does not contain any of the following substances except as CURRENTLY exempted by ELV II and RoHS and reported above:This product or product family does not contain any chemicals designated by the European Chemicals Agency (ECHA) as Substances of Very High Concern (SVHCs) underREACH. Please check the document at /_files/products_lead_free/RoHS_Product_List.pdf for the current compliance status.This data is based on information provided by our suppliers. We believe it to be correct but do not routinely validate it by measurement. It is for guidance only andDiodes Inc. does not guarantee its absolute accuracy or completenessAlloy 42Molding compound KTMC-1050GDie attached epoxy9005SP SOD-123 leadframe1.032 0.110 28.1112.99564.041 6.824。
进出口专业英语词汇(P1)
进出口专业英语词汇(P1)进出口专业英语词汇(P1)进出口专业英语词汇(P1)p-amine acetanilide 对氨基乙铣苯胺p-aminoazobenzene hydrochloride 对氨基偶氮苯盐酸盐p-aminophenol 对氨基苯酚p-anisidine 对氨基苯甲醚p-benzoquinone 对苯醌p-fluoro aniline 对氟苯胺p-hydroxy phenyl ethyl ketone 对羟基苯乙酮p-leuconiline 副品红隐色基p-n diode laser pn结二极管激光器p-n junction electroluminescent diode pn结电致发光二极管p-n junction laser pn结激光器p-n junction photodiode pn结光电二极管p-n junction semiconductor laser pn结半导体激光器p-nitroaniline 对硝基苯胺p-type semiconductor p型半导体paar calorimeter 帕尔量热器paar turbidimeter 帕尔浊度计paarlan 异乐灵pabnapar 帕布纳帕小花纹细布pace 节奏牌手表pacemaker analyser 起搏器分析器pacemaker pulse monitor 起搏器脉冲监视器pacemaker 起搏器pachas 帕查斯马海毛呢pachimeter 弹性切力极限测定计pachometer 测厚计pachras 帕克勒斯彩条粗布pachyma cocos 茯苓pachyma compound digestive tonic pill 保和丸pachymeter 测厚计pachyrhizua angulatus 粉葛pacific converter 丝束直接成条机pacific lamprey 太平洋鳗鱼pacific mackerel 太平洋鲐pacific yellowfin tuna 黄鳍金枪鱼pacific 太平洋牌手表pack cloth 打包布pack duck 打包帆布pack dyed yarn 筒子染色纱pack heating furnace 叠板加热炉pack mill 叠板轧机pack rope 打包绳pack sack 旅行背包pack take-off device 卸垛机pack thread 细绳pack tilting device 叠板翻转机pack twine 包扎麻绳pack-sack diamond drill 可背运的轻便金刚石钻机package counter 包装计数器package dryer 筒子烘燥机package linen 亚麻包装布package mill 打包用带钢轧机package tray 整装式塔盘package type air cooler 便携式空气冷却器package-drying machine 筒子纱干燥机packaged air conditioner 组合式空调器packaged boiler 移动式锅炉packaged gas turbine 快装式燃气轮机packaged reactor 装配式反应堆packaged rotary drum dryer 小型转筒式干燥机packaged tea 小包装茶packaged transistor 密封式晶体管packager 包装机packaging container of metal 金属包装容器packaging machine 包装机packaging paper 包装纸packaging production line 包装生产线packbasket 背篓packed column 填料塔packed-bed scrubber 填充床洗涤器packed-lantern-ring exchanger 填料-灯笼-环换热器packer 包装机packet adapter 分组适配器packet handler 分组处理器packet instamatic camera 袖珍型即拍即现照相机packet multiplexer 分组多路复用器packet repeater 分组中继器packet transmission controller 分组传输控制器packet voice and data multiplexer 分组话音与数据多路复用器packet voice multiplexer 分组话音多路复用器packing cloth 打包麻布packing machine 包装机packing machinery 包装机械packing maker 密封接合器packing material 包装材料packing needle 打包针packing paper 包装纸packing press 包装机packing ring 填料环packing rope 包装绳packing sheet 包装布packing tool 打包机packing washer 密封垫圈packless valve 无填料阀packplane 货舱可更换的飞机packsheet 高级打包麻布paco wool 羊驼毛pacputan wool 帕克普坦粗羊毛pacteron 帕克特龙铁碳磷母合金pad and cover for metal ironing board 金属熨衣板垫和套pad bearing 衬垫轴承pad dyer 轧染机pad lubricator 垫式润滑器pad printing machine 自动移印机pad quilted in diamond stitching 菱形绗褥pad quilted in zigzag stitching 之字绗褥pad relay 衰减器继电器pad with tape bindings 狭带捆边褥pad 拍纸簿padan 巴丹padauk furniture 红木家具padded back lining 黑背印花里子布padded jacket 夹袄padded shoulder 垫肩padder 垫整电容器padding capacitor 垫整电容器padding cloth 衬布padding condenser 垫整电容器padding machine 打底机padding mangle 轧染机padding 垫料paddings 西装麻衬布paddle agitator 桨式搅拌器paddle badminton 板羽球paddle blade stirrer 桨式搅拌器paddle blade type mixer 叶轮式混合机paddle board 蹼板paddle boat 明轮船paddle climb 攀架paddle drum bleacher 桨鼓漂白机paddle dryer 桨式干燥机paddle dyeing machine 桨叶式染色机paddle engine 明轮发动机paddle fan 离心式通风机paddle feeder 叶片式给料器paddle level switch 扳钮开关paddle loader 桨叶式装载机paddle mixer 叶片式搅拌机paddle passenger steamer 明轮客轮paddle steamer 明轮船paddle stirrer 桨式搅拌机paddle wheel agitator 叶轮式搅拌机paddle wheel elevator 叶轮式提升机paddle wheel water supplier 脚踏水车paddle wheel 桨轮paddle wool washing machine 桨叶式洗毛机paddle 乒乓球拍paddle-box 明轮壳paddle-type kneading machine 桨式混捏机paddle-wheel fan 叶轮式风扇paddock coat 男用紧腰骑马外衣paddy basket 谷箩paddy field cultivator 稻田中耕机paddy field harrow 水田耙paddy field plough 水田犁paddy field tractor 水田拖拉机paddy field weeder 稻田除草机paddy planter 水稻直播机paddy pounder 碾米机paddy transplanter 水稻插秧机paddy transplanting machine 水稻插秧机paddy 稻谷padimate 帕地马酯padisway silk 帕迭斯威棱纹绸padlette 帕德勒特凸花刺绣padlock 挂锁padmini 帕德米尼牌汽车padulasoy 棱纹花绸paejama 印度裤paeonia lactiflora pallas 白芍paeonia lactiflora 芍药paeonia suffruticosa 牡丹paesano hemp 帕瑟诺大麻page address register 页面。
Gold Tuning Solution, Part Number 8500-7000 Safety
Gold Tuning Solution, Part Number 8500-7000*************(24小时)化学品安全技术说明书GHS product identifier 应急咨询电话(带值班时间)::供应商/ 制造商:安捷伦科技贸易(上海)有限公司中国(上海)外高桥自由贸易试验区英伦路412号(邮编:200131)电话号码: 800-820-3278传真号码: 0086 (21) 5048 2818Gold Tuning Solution, Part Number 8500-7000化学品的推荐用途和限制用途8500-7000部件号:安全技术说明书根据 GB/ T 16483-2008 和 GB/ T 17519-2013GHS化学品标识:金调谐溶液, 部件号 8500-7000推荐用途:有关环境保护措施,请参阅第 12 节。
物质或混合物的分类根据 GB13690-2009 和 GB30000-2013紧急情况概述液体。
无色。
无气味的。
H290 - 可能腐蚀金属。
物理状态:颜色:气味:GHS危险性类别警示词:警告危险性说明:H290 - 可能腐蚀金属。
:防范说明预防措施:P234 - 仅在原包装内保存。
事故响应:P390 - 吸收溢出物,防止材料损坏。
安全储存:废弃处置:不适用。
标签要素象形图金属腐蚀物 - 类别 1物理和化学危险可能腐蚀金属。
健康危害没有明显的已知作用或严重危险。
::与物理,化学和毒理特性有关的症状皮肤接触食入吸入没有具体数据。
没有具体数据。
没有具体数据。
:::延迟和即时影响,以及短期和长期接触引起的慢性影响短期暴露潜在的即时效应:无资料。
潜在的延迟效应:无资料。
潜在的即时效应:无资料。
长期暴露潜在的延迟效应:无资料。
环境危害:没有明显的已知作用或严重危险。
其他危害:没有已知信息。
物质/混合物美国化学文摘社(CAS)编号/其它标识号:混合物就供应商当前已知,在所适用的浓度中,没有其它对健康或环境有害的成分需要在本章节报告。
Thermo Scientific
DATA SHEET 2D Labtainer BioProcess Containers2D Labtainer BioProcess Container (BPC) systemsWhether in standard or customized configurations, Labtainer BPCs are ideal for:• Dispensing, packaging, and storing cell culture media, buffers, and process liquids• Delivery of cell culture media or process liquids to small-scale bioprocess systems• Bioreactor and fermentation feed, sampling, and harvest • Chromatography feed and fraction collection• Storage and transport of bulk intermediate products, process intermediates, vaccine conjugates, and other biological productsSmall-volume liquid handling systems for cell culture and bioprocessingThermo Scientific ™ Labtainer ™ BioProcess Containers (BPCs) effectively address small-volume liquid handling needs. They range in size from 50 mL to 50 L, with avariety of standard configurations to meet most application needs. These Labtainer BPCs are space efficient, ergonomic, and constructed of Thermo Scientific ™Aegis ™ 5-14 and CX5-14 films. Product configurations cover a range of industry-standard connection systems, and handling systems are available for transport and storage.Standard productsStandard Labtainer BPCs are stocked for immediate delivery and are fully supported by our process and product validation program. For more information on our validation program, please refer to our validation guides for Aegis5-14 and CX5-14 films. Additionally, standard Labtainer BPCs have validated liquid shipping configurations.Standard configurations can be customized for optimal fit, form, and function to address process-specific applications using one of the industry’s largest libraries of qualified components.2D Labtainer BPCs are available with the Thermo Scientific ™ BioTitan ™ Retention Device. This universaltubing retention solution was designed to provide the best method for retaining flexible tubing on a barbed fitting and helps eliminate the risk of leaks and failure of the tubingconnection point.Table 1. Chamber information.50 mL–2 L, 2-port Labtainer BPC 2 L–50 L, 3-port Labtainer BPC2 L–50 L: Polyethylene ports are welded into the BPC seam: one 1/4 in. ID and two 3/8 in. ID ports on standard chamber.Table 2. Custom BPC options.Tubing type C-Flex™ (animal origin–free), silicone, PharMed™, or AdvantaFlex™Tubing size Specific lengths of 3.18–25.4 mm ID (1/8–1 in.); specific length depends on type of tubing chosenConnectors • Luer: 3.18–6.35 mm (1/8–1/4 in.) ID• CPC quick-connect: 6.35–19 mm (1/4–3/4 in.) ID• Steam-in-place connector: 6.35–19 mm (1/4–3/4 in.) ID• Tri-clamp: 3.18–25.4 mm (1/8–1 in.) ID• Mini tri-clamp: 3.18–12.7 mm (1/8–1/2 in.) ID• Aseptic connection and aseptic disconnection devices: all available sizes of Colder AseptiQuik™, Pall™ Kleenpak™, Cytiva ReadyMate™ DACOthers • Needle-free sample port (SmartSite™ or Clave™ products)• Filter capsule (Millipore™, Pall™, Sartorius™, Parker Bioscience™, Meissner™ products)Table 3. Presentation (as dry BPC systems).Outer packaging Supplied “flat-packed”—two polyethylene outer layersLabel • Description• Product code• Lot number• Expiration date on outer packaging and shipping containerSterilization Irradiation (25–40 kGy) inner side of outer packaging Shipping container Durable cardboard cartonDocumentation • Certificate of Analysis provided with each batch for each delivery • Certificate of Irradiation2 portsPack of 10Line 1Luer lock body connection, polypropyleneTubing: C-Flex; 30 cm (12 in.) lengthID x OD: 3.18 x 6.35 mm (0.125 x 0.25 in.)Line 2Luer lock insert connection, polypropyleneTubing: C-Flex; 30 cm (12 in.) lengthID x OD: 3.18 x 6.35 mm (0.125 x 0.25 in.) Line 1Luer lock body connection, polypropyleneTubing: C-Flex; 8 cm (3 in.) lengthID x OD: 6.35 x 10.92 mm (0.25 x 0.43 in.)Line 2Luer lock insert connection, polypropyleneTubing: C-Flex; 8 cm (3 in.) lengthID x OD: 6.35 x 10.92 mm (0.25 x 0.43 in.)Line 1Luer lock body connection, polypropyleneTubing: C-Flex; 30 cm (12 in.) lengthID x OD: 3.18 x 6.35 mm (0.125 x 0.25 in.)Line 2MPC insert, polycarbonateTubing: C-Flex; 8 cm (3 in.) lengthID x OD: 3.18 x 6.35 mm (0.125 x 0.25 in.)2 portsPack of 10Pack of 10Line 1Luer lock insert connection, polypropyleneTubing: C-Flex; 30 cm (12 in.) length ID x OD: 6.35 x 9.7 mm (0.25 x 0.38 in.)Line 2Luer lock body connection, polypropylene Tubing: C-Flex; 30 cm (12 in.) length ID x OD: 6.35 x 9.7 mm (0.25 x 0.38 in.)Line 3Luer lock body connection, polypropylene Tubing: C-Flex; 30 cm (12 in.) lengthID x OD: 3.18 x 6.35 mm (0.125 x 0.25 in.)Line 1MPC insert, polycarbonateTubing: C-Flex; 61 cm (24 in.) length ID x OD: 9.7 x 15.9 mm (0.38 x 0.63 in.)Line 2MPC body, polycarbonateTubing: C-Flex; 61 cm (24 in.) length ID x OD: 9.7 x 15.9 mm (0.38 x 0.63 in.)Line 3Luer lock body connection, polypropylene Tubing: C-Flex; 61 cm (24 in.) length ID x OD: 3.18 x 6.35 mm (0.125 x 0.25 in.)Line 1MPC insert, polycarbonateTubing: C-Flex; 30 cm (12 in.) length ID x OD: 9.7 x 12.7 mm (0.38 x 0.5 in.)Line 2MPC insert, polycarbonateTubing: C-Flex; 30 cm (12 in.) length ID x OD: 9.7 x 12.7 mm (0.38 x 0.5 in.)Line 3End plug, polypropyleneTubing: C-Flex; 8 cm (3 in.) lengthID x OD: 6.35 x 10.92 mm (0.25 x 0.43 in.)3 portsSingle pack3 portsSingle packSingle pack—edge portsLine 1MPC insert, polycarbonateTubing: C-Flex; 8 cm (3 in.) lengthID x OD: 9.6 x 12.7 mm (0.378 x 0.50 in.)Line 2MPC insert, polycarbonateTubing: C-Flex; 8 cm (3 in.) lengthID x OD: 9.6 x 12.7 mm (0.378 x 0.50 in.)Line 3Injection portTubing: C-Flex; 8 cm (3 in.) lengthID x OD: 6.35 x 9.53 mm (0.25 x 0.375 in.)Note: Aegis5-14 film equivalents for this product areavailable as custom configurations.Line 1MPC insert, polycarbonateTubing: C-Flex; 46 cm (18 in.) lengthID x OD: 3.18 x 6.35 mm (0.125 x 0.25 in.)Line 2MPC body, polypropyleneTubing: C-Flex; 61 cm (24 in.) lengthID x OD: 9.53 x 15.875 mm (0.375 x 0.625 in.)Line 3Luer lock body connection, polypropyleneTubing: C-Flex; 61 cm (24 in.) lengthID x OD: 9.53 x 15.875 mm (0.375 x 0.625 in.)3 portsSingle pack—pillow design withpanel portsFind out more at /bpcFor Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.© 2021 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unlessotherwise specified. C-Flex and PharMed are trademarks of Saint-Gobain Performance Plastics. AdvantaFlex is a trademark of NewAge Industries, Inc. AseptiQuik is a trademark of Colder Products Company. Pall and Kleenpak are trademarks of Pall Corporation. ReadyMate is a trademark of Cytiva. SmartSite is a trademark of Becton, Dickinson and Company. Clave is a trademark of Victus Inc. Millipore is a trademark of Merck KGaA, Darmstadt, Germany and/or its affiliates. Meissner is a trademark of Meissner Filtration Products. Parker Bioscience is a trademark of Parker Hannifin Corp. Rubbermaid is a trademark of Rubbermaid Incorporated. Sartorius is a trademark of Sartorius AG. Specifications, terms, and pricing are subject toIndustry-standard Rubbermaid ™ totes with corresponding lids are available. They can be used to protect Labtainer BPCs up to 20 L in size during use, transport, and storage. Use standard 50 L drums for 50 L Labtainer BPCs.Tray with lidFlat-bottom, linear low-density polyethylene (LLDPE) drum with lid。
ELASTOMERIC CLOSURES FOR INJECTIONS(注射剂橡胶塞)USP40
CALCULATIONCalculate the cobalamin content, expressed in m g of cyanocobalamin, of the portion taken for assay by the formula:R(C S /C U )(A U /A S )in which R is the quantity, in m g, of cyanocobalamin in the portion of the standard solution taken; C S and C U are the corrected average radioactivity values, expressed in counts per minute per mL, of the standard and assay solutions, respectively; and A Uand A Sare the absorbances determined at 361 nm of the assay and standard solutions, respectively.á381ñ ELASTOMERIC CLOSURES FOR INJECTIONSINTRODUCTIONElastomeric closures for containers used in the types of preparations defined in the general test chapter Injections and Im-planted Drug Products á1ñ are made of materials obtained by vulcanization (cross-linking) polymerization, polyaddition, or poly-condensation of macromolecular organic substances (elastomers). Closure formulations contain natural or synthetic elastomers and inorganic and organic additives to aid or control vulcanization, impart physical and chemical properties or color, or stabi-lize the closure formulation.This chapter applies to closures used for long-term storage of preparations defined in the general test chapter Packaging andStorage Requirements á659ñ, Injection Packaging . Such closures are typically used as part of a vial, bottle, or pre-fill syringe pack-age system.This chapter applies to closures formulated with natural or synthetic elastomeric substances. This chapter does not apply toclosures made from silicone elastomer; however, it does apply to closures treated with silicone (e.g., Dimethicone, NF ). Whenperforming the tests in this chapter, it is not required that closures be treated with silicone, although there is no restrictionprohibiting the use of siliconized closures.This chapter also applies to closures coated with other lubricious materials (e.g., materials chemically or mechanically bon-ded to the closure) that are not intended to, and in fact do not provide, a barrier to the base elastomer. When performing the tests, closures with lubricious nonbarrier coatings are to be tested in their coated state.The following comments relate solely to closures laminated or coated with materials intended to provide, or in fact function as, a barrier to the base elastomer (e.g., PTFE or lacquer coatings). It is not permissible to use a barrier material in an attempt to change a closure that does not meet compendial requirements to one that does conform. Therefore, all Physicochemical Tests apply to the base formula of such closures, as well as to the coated or laminated closure. To obtain Physicochemical Tests results, the tests are to be performed on uncoated or nonlaminated closures of the same elastomeric compound, as well as to the laminated or coated closure. The Functionality Tests apply to and are to be performed using the laminated or coated elasto-meric closure. Biological Tests apply to the lamination or coating material, as well as to the base formula. Biological Tests may be performed on the laminated or coated closure, or they may be performed on the laminate/coating material and the uncoa-ted or nonlaminated closures of the same elastomeric compound. In the latter case, the results are to be reported separately.The base formula used for physicochemical or biological tests intended to support the compendial compliance of a barrier-coated closure should be similar to the corresponding coated closure in configuration and size.For all Nephelometry, Turbidimetry, and Visual Comparison á855ñ tests performed on any closure type, it is important to docu-ment the closure being tested, including a full description of the elastomer, and any lubrication, coating, laminations, or treat-ments applied.This chapter states test limits for Type I and Type II elastomeric closures. Type I closures are typically used for aqueous prepa-rations. Type II closures are typically intended for nonaqueous preparations and are those which, having properties optimized for special uses, may not meet all requirements listed for Type I closures because of physical configuration, material of con-struction, or both. If a closure fails to meet one or more of the Type I test requirements, but still meets the Type II require-ments for the test(s), the closure is assigned a final classification of Type II. All elastomeric closures suitable for use with injecta-ble preparations must comply with either Type I or Type II test limits. However, this specification is not intended to serve as the sole evaluation criteria for the selection of such closures.It is appropriate to use this chapter when identifying elastomeric closures that might be acceptable for use with injectable preparations on the basis of their biological reactivity, their aqueous extract physicochemical properties, and their functionali-ty.The following closure evaluation requirements are beyond the scope of this chapter:—The establishment of closure identification tests and specifications—The verification of closure–product physicochemical compatibility—The identification and safety determination of closure leachables found in the packaged product—The verification of packaged product closure functionality under actual storage and use conditions326 á371ñ Cobalamin Radiotracer Assay / Chemical Tests USP 40The manufacturer of the injectable product (the end user) must obtain from the closure supplier an assurance that the com-position of the closure does not vary and that it is the same as that of the closure used during compatibility testing. When the supplier informs the end user of changes in the composition, compatibility testing must be repeated, totally or partly, depend-ing on the nature of the changes. Closures must be properly stored, cleaned for removal of environmental contaminants and endotoxins, and, for aseptic processes, sterilized prior to use in packaging injectable products.CHARACTERISTICSElastomeric closures are translucent or opaque and have no characteristic color, the latter depending on the additives used. They are homogeneous and practically free from flash and adventitious materials (e.g., fibers, foreign particles, and waste rub-ber.)IDENTIFICATIONClosures are made of a wide variety of elastomeric materials and optional polymeric coatings. For this reason, it is beyond the scope of this chapter to specify identification tests that encompass all possible closure presentations. However, it is the responsibility of the closure supplier and the injectable product manufacturer (the end user) to verify the closure elastomeric formulation and any coating or laminate materials used according to suitable identification tests. Examples of some of the ana-lytical test methodologies that may be used include specific gravity, percentage of ash analysis, sulfur content determination, FTIR-ATR test, thin-layer chromatography of an extract, UV absorption spectrophotometry of an extract, or IR absorption spec-trophotometry of a pyrolysate.TEST PROCEDURESElastomeric closures shall conform to biological, physicochemical, and functionality requirements both as they are shipped by the closure supplier to the injectable product manufacturer (the end user), and in their final ready-to-use state by the end user.For those elastomeric closures processed by the supplier prior to distribution to the end user, the supplier shall demonstrate compendial conformance of closures exposed to such processing and/or sterilization steps. Similarly, if elastomeric closures re-ceived by the end user are subsequently processed or sterilized, the end user is responsible for demonstrating the continued conformance of closures to compendial requirements subsequent to such processing and/or sterilization conditions (i.e., in their ready-to-use state). This is especially important if closures shall be exposed to processes or conditions that may signifi-cantly impact the biological, physicochemical, or functionality characteristics of the closure (e.g., gamma irradiation).For closures that are normally lubricated with silicone prior to use, it is permissible to perform physicochemical testing on nonlubricated closures, in order to avoid potential method interference and/or difficulties in interpreting test results. For clo-sures supplied with other lubricious nonbarrier coatings, all tests are to be performed using the coated closure.For closures coated or laminated with coatings intended to provide a barrier function (e.g., PTFE or lacquer coatings), physi-cochemical compendial tests apply to the uncoated base elastomer, as well as to the coated closure. In this case, suppliers are responsible for demonstrating physicochemical compendial compliance of the coated closure, as well as of the uncoated clo-sure, processed or treated in a manner simulating conditions typically followed by the supplier for such coated closures prior to shipment to the end user. The uncoated closure subject to physicochemical tests should be similar to the corresponding coat-ed closure in size and configuration. End users of coated closures are also responsible for demonstrating the continued physi-cochemical compendial conformance of the coated closure, processed or treated in a manner simulating conditions typically employed by the end user prior to use.In all cases, it is appropriate to document all conditions of closure processing, pretreatment, sterilization, or lubrication when reporting test results.Table 1 summarizes the testing requirements of closures, and the responsibilities of the supplier and the end user.Table 1Closure Types(As Supplied or Used)Test RequirementsPhysicochemical Tests Functionality Tests Biological TestsClosure with or withoutSilicone Coating• Tests are to be performed.• Tests are to be performed.• Tests are to be performed.• Silicone use is optional.• Silicone use is optional.• Silicone use is optional.• Responsibility: supplier and enduser• Responsibility: supplier and enduser• Responsibility: supplier and enduserClosures with LubriciousCoating (NonbarrierMaterial; Not Silicone)• Tests are to be performed oncoated closures.• Tests are to be performed oncoated closures.• Tests are to be performed oncoated closures.• Responsibility: supplier and enduser• Responsibility: supplier and enduser• Responsibility: supplier and enduserUSP 40Chemical Tests / á381ñ Elastomeric Closures for Injections 327Table 1 (Continued)Closure Types (As Supplied or Used)Test RequirementsPhysicochemical Tests Functionality Tests Biological TestsClosures with Barrier Coating • Tests are to be performed on coated closures.• Tests are to be performed on coated closures.• Tests are to be performed oncoated closures.• Responsibility: supplier and enduser • Responsibility: supplier and end user OR:AND:• Tests are to be performed on un-coated closures (base formula) andthe laminate/coating material (re-port results separately).• Tests are to be performed on un-coated closures (base formula).• Responsibility: supplier • Responsibility: supplier and end userBIOLOGICAL TESTSTwo stages of testing are indicated. The first stage is the performance of an in vitro test procedure as described in general test chapter Biological Reactivity Tests, In Vitro á87ñ. Materials that do not meet the requirements of the in vitro test are subjec-ted to the second stage of testing, which is the performance of the in vivo tests, Systemic Injection Test and Intracutaneous Test ,according to the procedures set forth in the general test chapter Biological Reactivity Tests, In Vivo á88ñ. Materials that meet therequirements of the in vitro test are not required to undergo in vivo testing.Type I and Type II closures must both conform to the requirements of either the in vitro or the in vivo biological reactivitytests. [N OTE —Also see the general information chapter The Biocompatibility of Material Used in Drug Containers, Medical Devices,and Implants á1031ñ.]PHYSICOCHEMICAL TESTSPreparation of Solution SPlace whole, uncut closures corresponding to a surface area of 100±10 cm 2into a suitable glass container. Cover the clo-sures with 200 mL of Purified Water or Water for Injection. If it is not possible to achieve the prescribed closure surface area (100±10 cm 2) using uncut closures, select the number of closures that will most closely approximate 100 cm 2, and adjust the volume of water used to the equivalent of 2 mL per each 1 cm 2 of actual closure surface area used. Boil for 5 minutes, and rinse five times with cold Purified Water or Water for Injection.Place the washed closures into a Type I glass wide-necked flask (see Containers—Glass á660ñ), add the same quantity of Puri-fied Water or Water for Injection initially added to the closures, and weigh. Cover the mouth of the flask with a Type I glass beaker. Heat in an autoclave so that a temperature of 121±2° is reached within 20 to 30 minutes, and maintain this tempera-ture for 30 minutes. Cool to room temperature over a period of about 30 minutes. Add Purified Water or Water for Injection to bring it up to the original mass. Shake, and immediately decant and collect the solution. [N OTE —This solution must be shaken before being used in each of the tests.]Preparation of BlankPrepare a blank solution similarly, using 200 mL of Purified Water or Water for Injection omitting the closures.A PPEARANCE OF S OLUTION (T URBIDITY /O PALESCENCE AND C OLOR )Determination of Turbidity (Opalescence)N OTE —The determination of turbidity may be performed by visual comparison (Procedure A ), or instrumentally using a suita-ble ratio turbidimeter (Procedure B ). For a discussion of turbidimetry, see Nephelometry, Turbidimetry, and Visual Comparison á855ñ. Instrumental assessment of clarity provides a more discriminatory test that does not depend on the visual acuity of the analyst.Hydrazine Sulfate Solution—Dissolve 1.0 g of hydrazine sulfate in water and dilute with water to 100.0 mL. Allow to stand for 4 to 6 hours.Hexamethylenetetramine Solution—Dissolve 2.5 g of hexamethylenetetramine in 25.0 mL of water in a 100-mL glass-stop-pered flask.Opalescence Stock Suspension—Add 25.0 mL of Hydrazine Sulfate Solution to the Hexamethylenetetramine Solution in the flask.Mix, and allow to stand for 24 hours. This suspension is stable for 2 months, provided it is stored in a glass container free from surface defects. The suspension must not adhere to the glass and must be well mixed before use.328 á381ñ Elastomeric Closures for Injections / Chemical Tests USP 40Opalescence Standard Suspension—Prepare a suspension by diluting 15.0 mL of the Opalescence Stock Suspension with water to 1000.0 mL. Opalescence Standard Suspension is stable for about 24 hours after preparation.Reference Suspensions—Prepare according to Table 2. Mix and shake before use. [N OTE—Stabilized formazin suspensions that can be used to prepare stable, diluted turbidity standards are available commercially and may be used after comparison with the standards prepared as described.]Table 2Reference Suspension A Reference Suspension B Reference Suspension C Reference Suspension D Standard of Opalescence 5.0 mL10.0 mL30.0 mL50.0 mLWater95.0 mL90.0 mL70.0 mL50.0 mL Nephelometric TurbidityUnits 3 NTU 6 NTU18 NTU30 NTU Procedure A: Visual Comparison—Use identical test tubes made of colorless, transparent, neutral glass with a flat base and an internal diameter of 15 to 25 mm. Fill one tube to a depth of 40 mm with Solution S, one tube to the same depth with water, and four others to the same depth with Reference Suspensions A, B, C, and D. Compare the solutions in diffuse daylight 5 mi-nutes after preparation of the Reference Suspensions, viewing vertically against a black background. The light conditions shall be such that Reference Suspension A can be readily distinguished from water and that Reference Suspension B can be readily distinguished from Reference Suspension A.R EQUIREMENT—Solution S is not more opalescent than Reference Suspension B for Type I closures, and not more opalescent than Reference Suspension C for Type II closures. Solution S is considered clear if its clarity is the same as that of water when examined as described above, or if its opalescence is not more pronounced than that of Reference Suspension A (refer to Table 3).Procedure B: Instrumental Comparison—Measure the turbidity of the Reference Suspensions in a suitable calibrated turbidime-ter (see á855ñ). The blank should be run and the results corrected for the blank. Reference Suspensions A, B, C, and D represent 3, 6, 18, and 30Nephelometric Turbidity Units (NTU), respectively. Measure the turbidity of Solution S using the calibrated turbidimeter.R EQUIREMENT—The turbidity of Solution S is not greater than that for Reference Suspension B (6NTU FTU) for Type I closures, and is not greater than that for Reference Suspension C (18NTU FTU) for Type II closures (refer to Table 3).Table 3Comparison MethodOpalescenceRequirements Procedure A (Visual)Procedure B(Instrumental)Type I closures No more opalescent than Suspension B No more than 6 NTUType II closures No more opalescent than Suspension CNo more than 18 NTU Determination of ColorColor Standard—Prepare a solution by diluting 3.0 mL of Matching Fluid O (see Color and Achromicity á631ñ) with 97.0 mL of diluted hydrochloric acid.Procedure—Use identical tubes made of colorless, transparent, neutral glass with a flat base and an internal diameter of 15 to 25 mm. Fill one tube to a depth of 40 mm with Solution S, and the second with the Color Standard. Compare the liquids in diffuse daylight, viewing vertically against a white background.Requirement—Solution S is not more intensely colored than the Color Standard.Acidity or AlkalinityBromothymol Blue Solution—Dissolve 50 mg of bromothymol blue in a mixture of 4 mL of 0.02 M sodium hydroxide and 20 mL of alcohol. Dilute with water to 100 mL.Procedure—To 20 mL of Solution S add 0.1 mL of Bromothymol Blue Solution. If the solution is yellow, titrate with 0.01 N sodium hydroxide until a blue endpoint is reached. If the solution is blue, titrate with 0.01 N hydrochloric acid until a yellow endpoint is reached. If the solution is green, it is neutral and no titration is required.Blank Correction—Test 20 mL of Blank similarly. Correct the results obtained for Solution S by subtracting or adding the vol-ume of titrant required for the Blank, as appropriate. (Reference Titrimetry á541ñ.)Requirement—Not more than 0.3 mL of 0.01 N sodium hydroxide produces a blue color, or not more than 0.8 mL of 0.01 N hydrochloric acid produces a yellow color, or no titration is required.AbsorbanceProcedure—[N OTE—Perform this test within 5 hours of preparing Solution S.] Pass Solution S through a 0.45-m m pore size filter, discarding the first few mL of filtrate. Measure the absorbance of the filtrate at wavelengths between 220 and 360 nm in USP 40Chemical Tests / á381ñ Elastomeric Closures for Injections 329a 1-cm cell using the blank in a matched cell in the reference beam. If dilution of the filtrate is required before measurement of the absorbance, correct the test results for the dilution.Requirement—The absorbances at these wavelengths do not exceed 0.2 for Type I closures or 4.0 for Type II closures.Reducing SubstancesProcedure— [N OTE —Perform this test within 4 hours of preparing Solution S .] To 20.0 mL of Solution S add 1 mL of diluted sulfuric acid and 20.0 mL of 0.002 M potassium permanganate. Boil for 3 minutes. Cool, add 1g of potassium iodide, and titrate immediately with 0.01 M sodium thiosulfate, using 0.25 mL of starch solution TS as the indicator. Perform a titration using 20.0 mL of blank and note the difference in volume of 0.01 M sodium thiosulfate required.Requirement—The difference between the titration volumes is not greater than 3.0 mL for Type I closures and not greater than 7.0 mL for Type II closures.Heavy MetalsProcedure—Proceed as directed for Method I under Heavy Metals á231ñ. Prepare the Test Preparation using 10.0 mL of Solu-tion S .Requirement—Solution S contains not more than 2 ppm of heavy metals as lead.Extractable ZincTest Solution—Prepare a Test Solution by diluting 10.0 mL of Solution S to 100 mL with 0.1 N hydrochloric acid. Prepare atest blank similarly, using the Blank for Solution S .Zinc Standard Solution—Prepare a solution (10 ppm Zn) by dissolving zinc sulfate in 0.1 N hydrochloric acid.Reference Solutions—Prepare not fewer than three Reference Solutions by diluting the Zinc Standard Solution with0.1 N hydrochloric acid. The concentrations of zinc in these Reference Solutions are to span the expected limit of the Test Solu-tion .Procedure—Use a suitable atomic absorption spectrophotometer (see Atomic Absorption Spectroscopy á852ñ) equipped with a zinc hollow-cathode lamp and an air–acetylene flame. An alternative procedure such as an appropriately validated inductively coupled plasma analysis (ICP) may be used.Test each of the Reference Solutions at the zinc emission line of 213.9 nm at least three times. Record the steady readings.Rinse the apparatus with the test blank solution each time, to ensure that the reading returns to initial blank value. Prepare a calibration curve from the mean of the readings obtained for each Reference Solution . Record the absorbance of the Test Solu-tion . Determine the ppm zinc concentration of the Test Solution using the calibration curve.Requirement—Solution S contains not more than 5 ppm of extractable zinc.AmmoniumAlkaline Potassium Tetraiodomercurate Solution—Prepare a 100 mL solution containing 11g of potassium iodide and 15g of mercuric iodide in water. Immediately before use, mix 1 volume of this solution with an equal volume of a 250g per L solution of sodium hydroxide.Test Solution—Dilute 5 mL of Solution S to 14 mL with water. Make alkaline if necessary by adding 1N sodium hydroxide,and dilute with water to 15 mL. Add 0.3 mL of Alkaline Potassium Tetraiodomercurate Solution, and close the container.Ammonium Standard Solution—Prepare a solution of ammonium chloride in water (1 ppm NH4). Mix 10 mL of the 1 ppmammonium chloride solution with 5 mL water and 0.3 mL of Alkaline Potassium Tetraiodomercurate Solution. Close the contain-er.Requirement—After 5 minutes, any yellow color in the Test Solution is no darker than the Ammonium Standard Solution (no more than 2 ppm of NH 4 in Solution S ).Volatile SulfidesProcedure—Place closures, cut if necessary, with a total surface area of 20±2 cm 2 in a 100-mL flask, and add 50 mL of a 20g per L citric acid solution. In the same manner and at the same time, prepare a control solution in a separate 100-mL flask by dissolving 0.154 mg of sodium sulfide in 50 mL of a 20g per L citric acid solution. Place a piece of lead acetate paper over the mouth of each flask, and hold the paper in position by placing over it an inverted weighing bottle. Heat the flasks in an autoclave at 121±2° for 30 minutes.Requirement—Any black stain on the paper produced by the test solution is not more intense than that produced by thecontrol substance.330 á381ñ Elastomeric Closures for Injections / Chemical Tests USP 40FUNCTIONALITY TESTSN OTE—Samples treated as described for preparation of Solution S and air dried should be used for Functionality Tests of Pene-trability, Fragmentation, and Self-Sealing Capacity. Functionality Tests are performed on closures intended to be pierced by a hy-podermic needle. The Self-Sealing Capacity test is required only for closures intended for multiple-dose containers. The needle specified for each test is a lubricated long bevel (bevel angle 12±2°) hypodermic needle1.PenetrabilityProcedure—Fill 10 suitable vials to the nominal volume with water, fit the closures to be examined, and secure with a cap. Using a new hypodermic needle as described above for each closure, pierce the closure with the needle perpendicular to the surface.Requirement—The force for piercing is no greater than 10N (1kgf) for each closure, determined with an accuracy of ±0.25 N (25gf).FragmentationClosures for Liquid Preparations—Fill 12 clean vials with water to 4 mL less than the nominal capacity. Fit the closures to be examined, secure with a cap, and allow to stand for 16 hours.Closures for Dry Preparations—Fit closures to be examined into 12 clean vials, and secure each with a cap.Procedure—Using a hypodermic needle as described above fitted to a clean syringe, inject into each vial 1 mL of water while removing 1 mL of air. Repeat this procedure four times for each closure, piercing each time at a different site. Use a new nee-dle for each closure, checking that it is not blunted during the test. Filter the total volume of liquid in all the vials through a single filter with a nominal pore size no greater than 0.5 m m. Count the rubber fragments on the surface of the filter visible to the naked eye.Requirement—There are no more than five fragments visible. This limit is based on the assumption that fragments with a diameter >50 m m are visible to the naked eye. In case of doubt or dispute, the particles are examined microscopically to verify their nature and size.Self-Sealing CapacityProcedure—Fill 10 suitable vials with water to the nominal volume. Fit the closures that are to be examined, and cap. Using a new hypodermic needle as described above for each closure, pierce each closure 10 times, piercing each time at a different site. Immerse the 10 vials in a solution of 0.1% (1g per L) methylene blue, and reduce the external pressure by 27kPa for 10 minutes. Restore to atmospheric pressure, and leave the vials immersed for 30 minutes. Rinse the outside of the vials.Requirement—None of the vials contain any trace of blue solution.á391ñ EPINEPHRINE ASSAYASSAYFerro-citrate solution:On the day needed, dissolve 1.5 g of ferrous sulfate in 200 mL of water to which have been added 1.0 mL of dilute hydrochloric acid (1 in 12) and 1.0 g of sodium bisulfite. Dissolve 500 mg of sodium citrate in 10 mL of this solution, and mix.Buffer solution:In a 50-mL volumetric flask mix 4.2 g of sodium bicarbonate, 5.0 g of potassium bicarbonate, and 18 mL of water (not all of the solids will dissolve at this stage). To another 18 mL of water add 3.75 g of aminoacetic acid and 1.7 mL of 6N ammonium hydroxide, mix to dissolve, and transfer this solution to the 50-mL volumetric flask containing the other mixture. Dilute with water to volume, and mix until solution is complete.Standard preparation:Transfer about 18 mg of USP Epinephrine Bitartrate RS, accurately weighed, to a 100-mL volumet-ric flask with the aid of 20 mL of sodium bisulfite solution (1 in 50), dilute with water to volume, and mix. Transfer 5.0 mL of this solution to a 50-mL volumetric flask, dilute with sodium bisulfite solution (1 in 500) to volume, and mix. [N OTE—Make the final dilution when the assay is carried out.] The concentration of USP Epinephrine Bitartrate RS in the Standard prepara-tion is about 18 m g per mL.Assay preparation:Transfer to a 50-mL volumetric flask an accurately measured volume of the Injection under assay, equivalent to about 500 m g of epinephrine, dilute with sodium bisulfite solution (1 in 500) to volume, if necessary, and mix. [N OTE—The final concentration of sodium bisulfite is in the range of 1 to 3 mg per mL, any bisulfite present in the Injection under assay being taken into consideration.]1Refer to ISO 7864, Sterile hypodermic needles for single use with an external diameter of 0.8 mm (21 Gauge).USP 40Chemical Tests / á391ñ Epinephrine Assay 331。
SensiMix
Shipping: On Dry/Blue Ice Catalog Numbers Batch No.: See vial Concentration: See vial QT605-05: 500 x 50 μL reactions: 10 x 1.25 mL Storage and Stability:SensiMix SYBR ®Hi --20 °C upon receipt. Excessive freeze/thawing is not recommended. Expiry:When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date on the outer box label.Quality Control:SensiMix SYBR ®Hi -ROX Kit and its components are extensively tested for activity,processivity, efficiency, heat activation, sensitivity, absence of nuclease contamination andabsence of nucleic acid contamination prior to release.Safety Precautions:Please refer to the material safety data sheet for further information.Notes:For research or further manufacturing use only. Trademarks:SensiMix, SensiFAST (Bioline Reagents Ltd), SYBR (Molecular Probes), ROX, LightCycler (Roche), StepOne (ABI), RotorGene (Qiagen), LightCycler (Roche).Kit componentsStore at –20 °CThe SensiMix SYBR ® Hi -ROX Kit has been optimized for use in SYBR ® Green -based qPCR on the real -time instruments listed in the following compatibility table, each of these instruments having the capacity to analyze the qPCR data with the passive reference signal either on or off. The kit is also compatible with several instruments that do not require the use of ROX, such as the BMS Mic, Qiagen Rotor -Gene ™ 6000, Bio -Rad CFX96 or Roche LightCycler ® 480.DescriptionThe SensiMix ™ SYBR ® Hi -ROX Kit is a high -performance reagent designed for superior sensitivity and specificity on various real -time instruments. The SensiMix SYBR ® Hi -ROX Kit employs a hot -start DNA polymerase, for high PCR specificity and sensitivity. SensiMix SYBR ® Hi -ROX is inactivated and possesses no polymerase activity during the reaction set -up, preventing non -specific amplification including primer -dimer formation.For ease -of -use and added convenience, SensiMix SYBR ® Hi -ROX is provided as a 2x master mix containing all the components necessary for real -time PCR (qPCR), including the SYBR ® Green I dye, dNTPs, stabilizers and ROX for optional use. As a ready -to -use premix, only primers and template need to be added. Kit compatibilityPrimers: the sequence and concentration of primer as well as the amplicon length can be critical for specific amplification, yield and overall efficiency of any qPCR. We strongly recommend taking the following into consideration when designing and running your PCR reaction:• use primer -design software, such as Primer3 or visual OMP TM (/primer3/ and DNA Software, Inc ; / respectively). Primers should have a melting temperature (Tm) of approximately 60 °C• optimal amplicon length should be 50-150 bp• a final primer concentration of 250 nM is suitable for most PCR conditions, however to determine the optimal concentration we recommend a primer titration in the range of 0.1–1 μM• use equimolar primer concentrations• when amplifying from cDNA use gene -specific primers. If possible use intron -spanning primers to avoid amplification from genomic DNATemplate: it is important that the DNA template is suitable for use in PCR in terms of purity and concentration. Also, the template needs to be devoid of any contaminating PCR inhibitors (e.g. EDTA). The recommended amount of template for PCR is dependent upon the type of DNA used. The following should be considered when using genomic DNA and cDNA templates:• Genomic DNA: use up to 1 μg of complex (e.g. eukaryotic) genomic DNA in a single PCR. We recommend using the ISOLATE Genomic II DNA Mini Kit (BIO -52066) for high yield and purity from both prokaryotic and eukaryotic sources• cDNA: the optimal amount of cDNA to use in a single PCR is dependent upon the copy number of the target gene. We suggest using 100 ng cDNA per reaction, however it may be necessary to vary this amount. To perform a two -step RT -PCR, we recommend using the SensiFAST cDNA Synthesis Kit (BIO -65053) for reverse transcription of the purified RNA. For high yield and purity of RNA, use the ISOLATE II RNA Mini Kit (BIO -52072)General considerationsTo help prevent any carry -over DNA contamination we recommend that separate areas be maintained for PCR set -up, PCR amplification and any post -PCR gel analysis. It is essential that any amplified PCR product should not be opened in the PCR set -up area.Website:/sensimixemail:****************************PI -50231 V11__________________________________________________________________________________________________________________________LICENSING INFORMATION2) Purchase of this product conveys a licence from Life Technologies to use this SYBR ® containing reagent in an end -user RUO assay. Parties wishing to incorporate this SYBR ® containing reagent into a downstream kit, should contact Life Technologies for SYBR ® Licencing information.Website:/sensimixemail:****************************Associated ProductsBioline Reagents Ltd UNITED KINGDOMTel: +44 (0)20 8830 5300 Fax: +44 (0)20 8452 2822Meridian Life Science Inc. USATel: +1 901 382 8716 Fax: +1 901.382.0027Bioline GmbH GERMANYTel: +49(0)3371 60222 00 Fax: +49(0)3371 60222 01Bioline (Aust) Pty. Ltd AUSTRALIATel: +61 (0)2 9209 4180 Fax: +61 (0)2 9209 4763Technical SupportIf the troubleshooting guide does not solve the difficulty you are experiencing, please contact Technical Support with details of reaction setup, cycling conditions and relevant data. Email: ********************************MgCl 2: The MgCl 2 concentration in the 1x reaction mix is 3 mM. In the majority of qPCR conditions this is optimal for both the reverse transcriptase and the hot -start DNA polymerase. If necessary, we suggest titrating the MgCl 2 to a maximum of 5mM.PCR controls: It is important to detect the presence of contaminating DNA that may affect the reliability of the data. Always include a no template control (NTC), replacing the template with PCR -grade water. When performing a two -step RT -qPCR, set -up a no RT control as the NTC for the PCR.ProcedureReaction mix composition: Prepare a PCR master mix. The volumes given below are based on a standard 50 μL final reaction mix and can be scaled accordingly.Optional ROX: The SensiMix ™SYBR Hi -ROX Kit is premixed with ROX (5-carboxy -X -rhodamine, succinymidyl ester), so that where necessary, ROX fluorescence can be optionally detected on certain real -time instruments. If your real -time instrument has the capability of using ROX and you wish to use this option, then this option must be selected by the user in the software.Troubleshooting GuideSuggested thermal cycling conditionsThe PCR conditions described below are suitable for the SensiMix ™ SYBR ® Hi -ROX Kit for the majority of amplicons and real -time PCR instruments. However, the cycling conditions can be varied to suit customer or machine -specific protocols. The critical step of the PCR is the 10 minute initial activation at 95 °C. The detection channel on the real -time instrument should be set to (SYBR ®) Green or FAM.*Non -variable parameterOptional analysis:After the reaction has reached completion refer to the instrument instructions for the option of melt -profile analysis.Website:/sensimixemail:****************************Website:/sensimixemail:****************************Troubleshooting Guide (Continued)。
BONDSTRAND
Bondstrand Glassfiber Reinforced Epoxy Piping SystemsHistorically, offshore production platform, drilling rig and FPSO owners and operators have had to face the grim reality of continuously replacing most metal piping because of severe corrosion. This has resulted in piping systems costing two or three times the original investment since steel and metal pipe systems are very costly to maintain. Bondstrand GRE pipe systems are the cost-effective, maintenance-free and lightweight solution that providescorrosion-free and erosion-free operation during the service life of the vessel.Durable and corrosion resistantBondstrand GRE is highly resistant to corrosion caused by (salt) water, chemicals, residues and bacteria.Similarly, it resists corrosion even in aggressive environments. Cathodic protection is not required.Lightweight – easy to installBondstrand GRE pipes weigh only a quarter to an eighth of steel pipes and are easy to install without the need of heavy nstallation equipment, welding or protective coating. For installation of GRE piping systems no ‘hot’ work is required.Low installation and operating costsInstallation costs of Bondstrand GRE pipe systems are less than that of carbon steel; total installed costs are comparable. Operating costs areThe many advantages of Bondstrand GRE pipe systemsreduced due to less energy needed to pump fluid through the smooth internal bore.Wide range of pipe systemsFiber Glass Systems offers a complete range of pipe systems in a variety of diameters and pressure classes for many different applications. Pipe systems are available in diameters up to 1000 mm (40 inch), and standard lengths up to 12 m (40 feet).No contaminationBondstrand GRE does not rust or scale. This prevents plugging of nozzles, valves and other components.WIDE RANGE OF APPLICATIONSOur corrosion-resistant piping systems can be used in a wide range of applications.Typical application areas are:COST COMPARISONCONVENTIONAL STEEL SYSTEMSTOTAL INSTALLED COST EQUALS T RADITIONAL STEEL PIPING A comparison of costs clearly shows the typical savings during the service life of the piping system.WIDE RANGE OF SOLUTIONSAs a leading producer Fiber Glass Systems offers the world’s mostcomprehensive range of glassfiber reinforced epoxy and phenolic pipe systems. Whether you need corrosion protection, fire protection, or a conductive system, Fiber Glass Systems offers t he right choice. Bondstrand GRE pipe series • Ballast water • Caissons • Cooling water • Disposal • Deluge (dry)• Drains • Drilling mud • Fresh water• Potable water • Produced water • Fire mains• Saltwater / seawater • Sanitary / sewage • Column piping • Vent linesSizes25-1000 mm (1–40 inch)Pressure classes up to 25 bar (365 psi)Internal liners available if neededConductive systems available if needed Joining systemsQuick-Lock™ and Taper/Taperadhesive bonded jointsSPECIFICATIONSISOThe objective of ISO 14692 is to provide the oil & gas industry and the supporting engineering and manufacturing industry with mutually agreed upon specifications and recommended practices for the design, purchase, manufacturing, qualification testing, handling, storage, installation, commissioning and operation of GRP (Glassfiber Reinforced Plastic - a generic terms including epoxy and other resins) piping systems.ISO 14692, part 2, 3 and 4 follow the individual phases in the life cycle of a GRP piping system, i.e. from design through manufacture tooperation. Each part is therefore aimed at the relevant parties involved in that particular phase.ISO 14692 is primarily intended for offshore applications on both fixed and floating topsides facilities, but it may also be used as guidancefor the specification, manufacture, testing and installation of GRE piping systems in other similar applications found onshore, e.g. produced water and firewater systems.IMOIn 1993, the International Maritime Organization (IMO) issued Resolution A.753(18) covering acceptance criteria for plastic materials in piping systems, appropriate design and installation requirements and fire test performance criteria for assuring ship safety. Major certifying bodies (such as Lloyd’s Register, Bureau Veritas, Det Norske Veritas, American Bureau of Shipping and United States Coast Guard) have adopted and implemented these Guidelines in their respective Rules and Regulations for the Classification of Ships and Floating Offshore facilities.All Bondstrand pipe series that are used in the marine/offshore industry are Type Approved by these major certifying bodies.Bondstrand Conductive Piping SystemsBondstrand conductive piping systems have been developed toprevent accumulation of potentially dangerous levels of static electrical charges.Pipe and flanges contain high strength conductive filaments; the fittings include a conductive liner. Combined with a conductive adhesive this provides an integral electrically continuous system.Grounding saddles can be bonded on the pipe. Integral grounding cables are then bolted to the steel structure to drain accumulatedcharges.Bondstrand conductive piping systems** Conductive version of Bondstrand 2000M (1) 2425 Bondstrand SeriesNote: All systems are available with a fire-protection layer.PRODUCT OVERVIEW(External pressure rating according to IMO Regulations)ENGINEERING CAPABILITIESWith manufacturing locations all over the world, Fiber Glass Systems has experienced teams of engineers supporting the customer with support design, engineering analysis, spool and isometric drawings and installation procedures.Fiber Glass Systems Engineering Service can include:• General engineering calculations such as support span, thrustloads, joint strength, collapse pressure and internal pressureratings, etc.• Design drawings, stress and surge analyses• Pipe Spool drawings from piping isometrics• Pipe support detailing• Material take offs (MTO)• Special product design for custom made parts• Expertise on international specification work towards approvalauthorities• Field service• Training to certify installersPREFABRICATIONBondstrand GRE systems are assembled using standard manufactured components. Spools can be pre-fabricated at the yard, or can be supplied from Fiber Glass Systems spooling operation or one of the network partners. The need for adhesive bonded joining on board can be limited.If pipe spacing is a constraint, Fiber Glass Systems can offer custom made spools to meet specific dimensions. Fiber Glass Systemsteam of piping engineers and fabricators can assist to ensure that custom-made spools are designed and fabricated to meet the project requirements.Pre-fabricated spools will reduce the number of field joints and provide greater reliability because of the high quality joints and testing at the Fiber Glass Systems factory.Installers, trained and certified by Fiber Glass Systems – according to IMO standards – can handle the complete installation.Fiber Glass Systems’ scope of supply may vary from material supply tocomplete ’turn-key’ projects.Prefabrication of custom made fiberglass spoolsCertified installation of Bondstrand piping systemTESTINGFIRE ENDURANCEEpoxy pipeUnder IMO Rules, Bondstrand epoxy products can be used for systems (normally water filled) without additional passive fire protection. Fire exposure will cause the outer surface of the pipe to char, but the functionality of the piping remains.Additional fire protectionDepending on the level of fire endurance required, epoxy pipe with enhanced fire resistance properties can be supplied meeting any of the following fire endurance requirements:- IMO L1- IMO L2- IMO FTP 2010- United States Coast Guard (USCG) W/D - USCG PFM 1-98- Jet Fire 30 OTI 95/634- Jet Fire 60 OTI 95/634Bondstrand fittings are tested to 1.5 times their pressure rating before they leave the factory or are used in spools. Small diameter fittings, to 150 mm (6 inch) are air tested, when possible.All others and the large diameter fittings are hydrotested. Fiber Glass Systems is the only manufacturer to conduct unrestrained hydro-test of fittings above 500 mm (20 inch) in diameter using self-energizing test plugs. Unrestrained testing is a more representative test as it simulates the actual conditions to which the pipe system is subjected in most Offshore installations.Fiber Glass Systems has extensive testing capabilities to meet special requirements. Comprehensive qualification testing is done onrepresentative sizes before manufacturing. Qualification test includes long-term hydrostatic test in accordance with ASTM D 2992, medium term survival test (1000 hour survival test) and short time burst test in accordance with ASTM D-1599. Mechanical and physical property testsof Bondstrand pipe can also be conducted.Hydrotesting of Bondstrand prefabricated pipe spool prior to shippingFire endurance testing of Bondstrand fiberglass pipe and fittingJOINING SYSTEMSQuick™ Lock- An adhesive-bonded joint with straight spigot and tapered bell. The integral pipe stop in theQuick-Lock bell provides accurate laying lengths in close tolerance piping. Available in sizes 50-400 mm (2-16 in).Taper x Taper - An adhesive-bonded joint with matching tapered male and female ends offering superiorjoint strength by controlled adhesive thickness. Available in sizes 50-1000 mm (2-40 in).Flanges - One-piece flanges and Stub-end flanges with movable rings. Available in sizes 50-1000 mm (2-40 in).Double O-Ring - A mechanical joint offering quick assembly between male and female ends. Two “O” ringsare employed to provide sealing. Available in sizes 50-900 mm (2-36 in).Fittings - Standard filament-wound Couplings; 30°, 45°, 60°, and 90° Elbows; Tees and Reducing Tees;Concentric Reducers; Flanges and Nipples. Standard Flanges are available with the following drilling: ANSIB16.5 Class 150 & 300, DIN, ISO and JIS. Other drilling patterns are available on request. Available in sizes50-1000 mm (2-40 inch)Fiber Glass Systems17115 San Pedro Avenue, Ste 200San Antonio, Texas 78232 USA Phone: 210 477 7500Fax: 210 477 7560National Oilwell Varco has produced this brochure for general information only, and it is not intended for design purposes. Although every effort has been made to maintain the accuracy and reliability of its contents, National Oilwell Varco in no way assumes responsibility for liability for any loss, damage or injury resulting from the use of information and data herein nor is any warranty expressed or implied. Always cross-reference the bulletin date with the most current version listed at the web site noted in this literature.© 2017 National Oilwell Varco All Rights Reserved MOS1100 October 2017。
东洋纺织株式会社试剂说明书
manual THUNDERBIRD Probe qPCR Mix 0910 A4250K THUNDERBIRD™ Probe qPCR MixQPS-101T 1 mL x 1QPS-101 1.67 mL x 3Store at -20°C, protected from lightContents[1] Introduction[2] Components[3] Primer/Probe design[4] Template DNA[5] Protocol1. Standard reaction set up2. Cycling conditions2-1. Real-time PCR conditions using Applied Biosystems 7900HT2-2. Real-time PCR conditions using Roche LightCycler 1.1[6] Related ProtocolcDNA synthesis[7] Troubleshooting[8] Related productsC AUTIONAll reagents in this kit are intended for research purposes only. Do not use for diagnosis or clinical purposes. Please observe general laboratory precautions and observe safety procedures while using this kit.-LightCycler™ is a trademark of Idaho Technology, Inc. and Roche Molecular Systems, Inc.-TaqMan® is a registered trademark of Roche Molecular Systems, Inc.-SYBR® is a registered trademark of Roche Molecular Systems, Inc.JAPAN CHINATOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140www.toyobo.co.jp/e/bio 1JAPAN CHINATOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140www.toyobo.co.jp/e/bio********************1[ 1 ] Introduction [ 2 ] Components DescriptionTHUNDERBIRD™ Probe qPCR Mix is a highly efficient 2x Master Mix for real-time PCR using TaqMan® probes. The master mix contains all required components, except for ROX reference dye, probe and primers (50x ROX reference dye is individually supplied with this kit). The master mix facilitates reaction setup, and improves the reproducibility of experiments.This product is an improved version of Realtime PCR Master Mix (Code No. QPK-101). In particular, reaction specificity and PCR efficiency is enhanced.Features-High specificityThe specificity for the detection of low-copy targets is improved.-Homogeneous amplificationThe dispersion of PCR efficiency between targets is reduced by a new PCR enhancer*. (*Patent pending)-Broad dynamic rangeHigh specificity and effective amplification enable the detection of a broad dynamic range.-Compatibility for various real-time cyclers.The reagent is applicable to most real-time cyclers (i.e. Block type and glass capillary type). Because the 50x ROX reference dye is individually supplied with this kit, the kit can be applied to real-time cyclers that require a passive reference dye.-Hot start PCRThe master mix contains anti-Taq DNA polymerase antibodies for hot start technology. The antibodies are easily inactivated in the first denaturation step, thereby activating the DNA polymerase.About the fluorescent probe detection systemThe TaqMan® probe system utilizes fluorescence emission from the probes. The probes hybridize to the target amplicons and then emit fluorescence upon degradation by the 5'-3' exonuclease activity of Taq DNA polymerase. This type of detection system can achieve higher specificity in real-time PCR assays than the SYBR® Green I detection system.This kit includes the following components for 40 reactions (QPS-101T) and 200 reactions (QPS-101), with 50 μl per reaction. All reagents should be stored at -20°C.<QPS-101T>THUNDERBIRD™ Probe qPCR Mix 1 ml x 150x ROX reference dye 50 μl x 1JAPAN CHINATOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140www.toyobo.co.jp/e/bio********************2[ 3 ] Primer/Probedesign <QPS-101>THUNDERBIRD™ Probe qPCR Mix 1.67 ml x 350x ROX reference dye 250 μl x 1Notes:-THUNDERBIRD™ Probe qPCR Mix can be stored, protected from light, at 2-8°C for up to 3 months. For longer storage, this reagent should be kept at -20°C and protected from light. No negative effect was detected by 10 freeze-thaw cycles of THUNDERBRID™ Probe qPCR Mix. This reagent does not contain the ROX reference dye.-50x ROX reference dye can be stored, protected from light, at 2-8°C or -20°C. For real-time cyclers that require a passive reference dye, this reagent must be added to the reaction mixture at a concentration of 1x or 0.1x. The master mix solution with the ROX reference dye can be stored, protected from light, at 2-8°C for up to 3 months. For longer storage, this reagent should be kept at -20°C and protected from light. The pre-mixed reagents can be prepared according to the following ratios. [5] Table 1 shows the optimal concentration of the ROX dye.1x solutionTHUNDERBIRD™ Probe qPCR Mix : 50x ROX reference dye = 1.67 ml : 66.8 μl THUNDERBIRD™ Probe qPCR Mix : 50x ROX reference dye = 1 ml : 40 μl0.1x solutionTHUNDERBIRD™ Probe qPCR Mix : 50x ROX reference dye = 1.67 ml : 6.7 μl THUNDERBIRD™ Probe qPCR Mix : 50x ROX reference dye = 1 ml : 4 μlFor real-time cyclers that do not require a passive reference dye, THUNDERBIRD™ Probe qPCR Mix without the ROX reference dye can be used.1. Primer conditionsHighly sensitive and quantitative data depend on primer design. The primer should be designed according to the following suggestions;-Primer length: 20-30 mer-GC content of primer: 40-60%-Target length: ≤ 200 bp (optimally, 80-150 bp)-Melting temperature (Tm) of primers: 60-65°C-Purification grade of primers: Cartridge (OPC) grade or HPLC gradeNotes:-Longer targets (>200 bp) reduce efficiency and specificity of amplification.-Tm of the primers can be flexible, because the Tm value depends on the calculation formula.JAPAN CHINATOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140www.toyobo.co.jp/e/bio********************3[ 4 ] Template DNA 2. Fluorescent probeThe probes should be designed according to the guidelines of each probe system. Because insufficiently purified probes may inhibit the reaction, HPLC-grade probes should be used.The following DNA samples can be used as templates.1. cDNANon-purified cDNA, generated by reverse transcription reactions, can be used directly for real-time PCR using THUNDERBIRD™ Probe qPCR Mix. Up to 10% of the volume of a cDNA solution can be used for a real-time PCR reaction. However, excess volume of the cDNA may inhibit the PCR. Up to 20% (v/v) of the cDNA solution from ReverTra Ace® qPCR RT Kit (Code No. FSQ-101) can be used for real-time PCR (see [6]).2. Genomic DNA, Viral RNAGenomic DNA and viral RNA can be used at up to 200 ng in 50 μl reactions.3. Plasmid DNAAlthough super-coiled plasmids can be used, linearized plasmid DNA produces more accurate assays. The copy number of the plasmid DNA can be calculated by the following formula.Copy number of 1μg of plasmid DNA = 9.1 x 1011 / Size of plasmid DNA (kb)JAPAN CHINATOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140www.toyobo.co.jp/e/bio********************4[ 5 ] Protocol1. Reaction mixture setupReaction volume FinalReagent50µl20µlConcentrationDWXµlXµlTHUNDERBIRD™ Probe qPCR Mix 25 µl 10 μl 1xForward Primer 15 pmol 6 pmol 0.3 μM*1Reverse Primer 15 pmol 6 pmol 0.3 μM*1TaqMan® Probe 10 pmol 4 pmol 0.2 μM*150x ROX reference dye 1μl / 0.1 μl 0.4μl / 0.04μl 1x / 0.1x*2DNA solution Y µl Y µlTotal50µl20µlNotes:*1 Primer / probe concentration should be determined according to the manufacturer’sinstructions.Higher primer concentration tends to improve the amplification efficiency, and lowerprimer concentration tends to reduce the non-specific amplification. The primerconcentration should be set between 0.2-0.6 μM.*2 50x ROX reference dye must be added when using real-time cyclers that require apassive reference dye, according to Table 1. Table 1 shows the optimum concentrationof the ROX reference dye. This dye is not necessary for real-time cyclers that do notrequire a passive reference dye.Table 1 Recommended ROX dye concentrationReal-time cycler Optimal dye concentration(dilution ratio)Applied Biosystems 7000, 7300, 7700, 7900HT etc. 1x (50:1)Applied Biosystems 7500, 7500Fast,Stratagene cyclers (Optional) etc.0.1x (500:1)Roche’ cyclers, Bio-Rad cyclers, BioFlux cyclers etc. Not requiredNotes:The ROX dye in Realtime PCR Master Mix (Code No. QPK-101) corresponds to 1xconcentration.JAPAN CHINA TOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140 www.toyobo.co.jp/e/bio********************52. PCR cycling conditionsThe following table shows the recommended thermal conditions using primers designed according to the recommended primer and probe conditions described in [3]. Almost all targets can also be amplified using the ongoing conditions with other real-time PCR reagents.*1Due to the anti-Taq antibody hot start PCR system, the pre-denaturation can be completed within 60 sec. The pre-denaturation time should be determined according to the recommendations of each real-time cycler. If the optimal pre-denaturation time cannot be determined, the time should be set at 60 sec.Table 2 The recommended pre-denaturation time for each real-time cycler Real-time cycler Pre-denaturation time High speed cycler (e.g. Applied Biosystems 7500Fast) 20 sec Capillary cycler (e.g. Roche LightCycler™ 1.x, 2.0) 30 secGeneral real-time cyclers (Applied Biosystems 7700, 7500,7900HT (normal block), Stratagene cyclers, BioFlux cyclers 60 sec *2The following table shows the optimal denaturation times for each real-time cycler. If the optimal denaturation time cannot be determined, the time should be set at 15 sec.Table 3 The recommended denaturation time for each real-time cycler Real-time cycler denaturation time High speed cycler (e.g. Applied Biosystems 7500Fast) 3 sec Capillary cycler (e.g. Roche LightCycler™ 1.x, 2.0) 5 secGeneral real-time cyclers (Applied Biosystems 7700, 7500,7900HT (normal block), Stratagene cyclers, BioFlux cyclers 15 sec *3Insufficient amplification may be improved by decreasing the extension temperature, and non-specific amplification (e.g. abnormal shapes of the amplification curve at low template concentrations) may be reduced by increasing the extension temperature. The extension temperature should be set at 56-64°C. *4If the target size is smaller than 300 bp, the extension time can be set at 30 sec on almost all real-time cyclers. Instability of the amplification curve or variation of data from each well may be improved by setting the extension time at 45-60 sec. Some real-time cyclers or software need over 30 sec for the extension step. In these cases, the time should be set according to each instruction manual (e.g. Applied Biosystems 7000/73000: ≥ 31 sec; Applied Biosystems 7500: ≥ 35 sec.).<3-step cycle> Temperature Time Ramp Pre-denaturation:95°C 20-60 sec *1Maximum Denaturation:95°C 1-15 sec *2 MaximumExtension: 60°C *3 30-60 sec *4 Maximum (data collection should be set at the extension step)JAPAN CHINA TOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140 www.toyobo.co.jp/e/bio********************62-1. Real-time PCR conditions using Applied Biosystems 7900HT(Normal block type, software version 2.2.2)The following is an example of a TaqMan ® assay using Applied Biosystems 7900HT.(1) The cycling parameters should be set according to the following “Thermal CyclerProtocol” window under the “Instrument” tab.Notes:- Appropriate sample volumes should be set. - ≥ 45 sec is necessary for the extension step.(2) Click the “Data collection” tab.(3) Insert the PCR plate(4) Start the programJAPAN CHINATOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140www.toyobo.co.jp/e/bio********************72-2. Real-time PCR conditions using Roche LightCycler 1.1(Software version 3.5)The following is an example of a TaqMan® probe assay using Roche LightCycler 1.1. (1)The cycling parameters should be set according to the following window. Analysisand Acquisition mode of the initial denaturation step must be set at “None”. (2)The cycling parameters should be set according to the following window. Analysismode of the PCR step must be set at “Quantification”. Acquisition modes of 95°C and 60°C must be set at “None” and “Single”, respectively.JAPAN CHINATOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140www.toyobo.co.jp/e/bio********************8(3)The cycling parameters should be set according to the following window. Analysisand Acquisition mode of the cooling step must be set at “Non”.(4) Insert the capillaries in the carousel, and start the cycling program.JAPAN CHINATOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140www.toyobo.co.jp/e/bio********************9[ 6 ] Related Protocol1. cDNA synthesiscDNA synthesized by various cDNA synthesis reagents can be used withTHUNDERBIRD™ Probe qPCR Mix. However, cDNA synthesized by a reagentspecialized for real-time PCR can increase sensitivity.ReverTra Ace® qPCR RT Kit (Code No. FSQ-101) is a cDNA synthesis kit suitable forreal-time PCR. Here, the protocol with ReverTra Ace® qPCR RT Kit is described.However, for the detailed protocol, please refer to the instruction manual of the kit.(1) Denaturation of RNAIncubate the RNA solution at 65°C for 5 min and then chill on ice.Notes:-This step can be omitted. But this step may increase the efficiency of the reversetranscription of RNA, which forms secondary structures.-Do not add 5x RT Buffer and/or enzyme solution at this step.(2) Preparation of the reaction solutionReagent V olume (amount)Nuclease-freeWaterXµl5x RT Buffer 2 µlPrimerMix 0.5µlEnzymeMix 0.5µlRNAsolution 0.5pg-1 µgTotal10µl(3) Reverse transcription reaction-Incubate at 37°C for 15 min. <Reverse transcription>-Heat at 98°C for 2 min. <Inactivation of the reverse transcriptase>-Store at 4°C or -20°C.**This solution can be used in the real-time PCR reaction directly or after dilution.Notes:The above temperature conditions are optimized for ReverTra Ace® qPCR RT Kit.JAPAN CHINATOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140www.toyobo.co.jp/e/bio********************10[ 7 ] TroubleshootingSymptom Cause SolutionLoss of linearity in the high cDNA/DNA concentration region. Inhibition by the components in thecDNA/DNA solution.-DNA: The DNA sample may contain PCRinhibitors. The DNA samples should berepurified.-cDNA: The components in the cDNA synthesisreagent may inhibit the PCR reaction. The cDNAsample should be used after dilution.The template DNA is insufficient. When the DNA/cDNA copy number is lower than10 copies per reaction, the linearity of the reactiontends to be lost. The template concentrationshould be increased.Adsorption of the DNA to the tubewall.The diluted DNA templates tend to be absorbedonto the tube wall. Dilution should be performedjust prior to experiments.Lost of linearity or lower signal in the lowDNA/cDNAconcentration region. Competition with primer dimerformation. In the probe assay, primer dimers are not detected. However, dimer formation may reduce the amplification efficiency of the target, especially for reactions at low template concentration. The reaction conditions should be optimized or the primer sequences should be changed.Loss of linearity of the amplification carves. Competition with non-specificamplification.In the probe assay, non-specific amplification isnot detected. However, non-specific amplificationmay reduce the amplification efficiency of thetarget. The reaction conditions should beoptimized or the primer sequences should bechanged.Inappropriate cycling conditions. Optimize the cycling conditions according to [5]. Degradation of the primers. Fresh primer solution should be prepared.The PCR efficiency is lower than 90% (slope:<-3.6) The calculation of the PCRefficiency is inappropriate. The Ct value on the linear region should be used to calculate PCR efficiency.The PCR efficiency is higher than 110%. The calculation of the PCRefficiency is inappropriate.The Ct value on the linear region should be usedto calculate PCR efficiency.Poor purification of the templateDNA.Low-purity DNA may contain PCR inhibitors.Re-purify the DNA samples.Absorption of the template DNA tothe tube wall.Diluted DNA templates tend to be absorbed ontothe tube wall. Dilution of the templateDNA/cDNA should be performed just prior toexperiments.Plasmid DNA or PCR product isused as a template.In general, plasmid DNA or PCR product is usedat low concentration. Diluted DNA templates tendto be absorbed onto the tube wall. Dilution of thetemplate DNA/cDNA should be performed justprior to experiments. Dilution with a carriernucleic acid solution (Yeast RNA) is alsoeffective in improving linearity.Inappropriate thermal conditions. Optimize the thermal conditions according to [5].Reproducibility is notgood.Low purity of the primers or probes.Different lots of primers or probes may showdifferent results. When the lot is changed, priortesting of the primer or probe should beperformed.JAPAN CHINATOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140www.toyobo.co.jp/e/bio********************11Symptom Cause SolutionContamination or carry over of the PCR products. Change the contaminated reagent.Amplification from the non-template control(NTC). Inappropriate settings offluorescence measurement, such asin the case of multiplex PCR. In multiplex experiments, inappropriate setting of fluorescence measurement may cause the detection of noise by the cross talk of fluorescent dyes. Settings should be reconfirmed.Excessive amount of ROX reference dye. Excessive amount of ROX reference dye may cause low signal. 50x ROX reference dye should be used according to [5] Table 1.Inappropriate settings of fluorescence measurement. Settings should be confirmed according to the instruction manual of each detector.Low purity of fluorescent probes. Low purity of the probe may increase the baseline. HPLC grade probes should be used.Excessive intensity of the quencher Dye. Certain quenchers (e.g. TAMRA) may cause a higher baseline because of its fluorescence. Use of a non-fluorescent quencher may improve the high baseline.Degradation of the probe. Store the probes according to the manufacture’srecommendations.Insufficient fluorescence measurement time. Certain detection systems require a longer time to detect the fluorescent signal. Longer extension (measurement) time (45-60 sec) may improve the unstable signal.Low amplificationcurve signal /Unstable amplificationcurve signal.Insufficient reaction volume. Low reaction volume may cause an unstablesignal. Increase the reaction volume.JAPAN CHINATOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140www.toyobo.co.jp/e/bio********************12[ 8 ] Related productsProduct name Package Code No.High efficiency real-time PCR master mix THUNDERBIRD™ SYBR® qPCR Mix 200reactionsQPS-201High efficiency cDNA synthesis kit for real-time PCR ReverTra Ace® qPCR RT Kit 200reactionsFSQ-101One-step Real-time PCR master mix for probe assayRNA-direct™ Realtime PCR Master Mix 0.5 mL x 20.5 mL x 5QRT-101TQRT-101One-step Real-time PCR master mix for SYBR® Green assayRNA-direct™ SYBR® Realtime PCR Master Mix 0.5 mL x 20.5 mL x 5QRT-201TQRT-201JAPAN CHINA TOYOBO CO., LTD. TOYOBO Bio-Technology, CO., LTD.Tel(81)-6-6348-3888 Tel(86)-21-58794900.4140 www.toyobo.co.jp/e/bio********************NOTICE TO PURCHASER: LIMITED LICENSEA license to perform the patented 5’ Nuclease Process for research is obtained by the purchase of (i) both Authorized 5' Nuclease Core Kit and Licensed Probe, (ii) a Licensed 5’ Nuclease Kit, or (iii) license rights from Applied Biosystems.This product is an Authorized 5’ Nuclease Core Kit. Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155, 5,677,152, 5,773,258, 5,407,800, 5,322,770, 5,310,652, 5,210,015, 5,487,972, and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. Separate purchase of a Licensed Probe would convey rights under the applicable claims of US Patents Nos. 5,538,848, 5,723,591, 5,876,930, 6,030,787, 6,258,569, 5,804,375 (claims 1-12 only), and 6,214,979, and corresponding claims outside the United States. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained from the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.。
SHI Cryopump Product Catalogue说明书
Sumitomo Heavy Industries, Ltd. (SHI) has a tradition of excellence and innovation that spans over 400 years. From its very beginning as a small shop selling medicines and books in Kyoto, Japan in the early 17th century, to its current status as a diverse, $6 billion corporation, SHI has continued to grow and flourish in an ever-changing international market.SHI’s acquisition of IGC-APD Cryogenics, Inc. in 2002 brought together two of the world’s leading cryogenic companies to form the SHI Cryogenics Group, with an unsurpassed tradition of design, development and success in the manufac-ture of cryogenic equipment.SHI Cryopumps continue this tradition by supporting both global research & development as well as state-of-the-art technologies. Today, applications of cryogenic technologies can be found in our daily lives. SHI Cryopumps are used directly or in the manufacturing of many of the world’s semiconductor, telecommunications, electronics, vacuum-coat-ed, and custom laboratory equipment and products.SHI offers a wide range of Cryopump products. Marathon® CP Series Cryopumps are offered with stan-dard and low profile enclosures, several flange options and manual and automatic features. They can be serviced in-situ without breaking vacuum or removing the pump from the chamber.The SICERA® Cryopump uses SHI proprietary inverter technology to reduce customerenergy costs. The resulting savings and increased production efficiency makeSICERA® ideal for semiconductor-related manufacturing.SHI Cryopumps are built in world-class manufacturing facilities us-ing Six Sigma manufacturing processes and process capabilitiesand analysis. The result is a product portfolio that offersflexibility, high reliability and is supported by aglobal sales, service and supportnetwork.Cryopump ModelMarathon® CP SICERA®CP-8CP-8LP CP-250LP CP-12CP-16CP-20KZ-8L KZ-12LAir (liters/second)1,5001,8003,0003,6004,8009,7001,5003,300 Water (liters/second)4,2004,2006,3009,56017,30029,1004,0009,500 Argon (liters/second)1,2501,5002,5003,1004,1008,3001,2003,500 Hydrogen (liters/second)2,3003,0005,0007,30012,00014,0002,2005,500 Argon Throughput (torr liters/second)11.011.011.012.611.411.38.811.3 Argon Capacity (standard liters)1,2001,6001,6002,0005,5006,0001,0002,000 Hydrogen Capacity (standard liters)2523305050331235 Crossover Rating (torr-liters)220220300650500400150150Weight35 lbs.(16.8 kg)39.5 lbs.(17.9 kg)44 lbs.(20 kg)90 lbs.(41 kg)110 lbs.(50 kg)170 lbs.(77 kg)70.6 lbs.(32 kg)88.2 lbs.(40 kg)Zephyr®•••HC-4E1•••Masatomo Sumitomo, founder of the Sumitomo family, opens a shopdealing in medicines and books in Kyoto, Japan17th Century Discovery of Besshi Copper Mine—Sumitomo receives exclusivemining rights1690Precursor to Sumitomo Heavy Industries, Ltd. established as amachinery production and repair facility at the Besshi Mine Plant1888Establishment of Sumitomo Machinery Works, Ltd.19341959Precursor to APD Cryogenics established as Space and MissileDepartment of Air Products in Allentown, Pennsylvania, USASumitomo establishes its cryogenics business at the Hiratsuka Research Laboratory in Hiratsuka City, near Tokyo. 1962Renamed the Advanced Product Development Department of AirProducts1968Introduces Displex ® cryocooler systems Merger between Sumitomo Machinery and Uraga Heavy Industriesresults in the establishment of Sumitomo Heavy Industries, Ltd.19691976Pioneers current generation cryopump technologyMerger with Nittoku Metal Industries results in the establishment of the1982APPLICATIONSSHI’s Cryopump systems are specifically designed to meet the needs of high vacuum processes, and are used in the manufacture of a variety of products. Typical applications for cryopumps include:Performance Specifications Performance Specifications Available Configurations• ANSI 6”, ISO 200 or CF 10” FlangeOptions• Standard Manual Operation• Optional Fully-Automated Operationwith Marathon ® Cryopump Controller• Two (2) cryopumps operating with one(1) HC-8E4 or F-70L/H Compressor• Displex ® Technology Standard Scope of Supply • CP-8 Cryopump • Zephyr ®, HC-4E1, HC-8E4 or F-70L/H Compressor • 10’ Flexible Gas Lines • 10’ Cold Head Cable • Tool Kit Available Configurations• Standard Low Profile Design in Left orRight Hand Configurations• ANSI 6”, ISO 200 or CF 10” FlangeOptions• Standard Manual Operation• Optional Fully-Automated Operationwith Marathon ® Cryopump Controller• Two (2) cryopumps operating with one(1) HC-8E4 or F-70L/H Compressor• Displex ® Technology Standard Scope of Supply • CP-8LP Cryopump • Zephyr ®, HC-4E1, HC-8E4 or F-70L/H Compressor • 10’ Flexible Gas Lines • 10’ Cold Head CablePerformance Specifications Performance Specifications Available Configurations• Standard Low Profile Design in Left orRight Hand Configurations• ISO 250 Flange• Standard Manual Operation• Optional Fully-Automated Operationwith Marathon ® Cryopump Controller• Two (2) cryopumps operating with one(1) HC-8E4 or F-70L/H Compressor• Displex ® Technology Standard Scope of Supply • CP-250LP Cryopump • Zephyr ®, HC-4E1, HC-8E4 or F-70L/H Compressor • 10’ Flexible Gas Lines • 10’ Cold Head Cable • Tool Kit Available Configurations• ANSI 10”, ISO 320 or CF 14” FlangeOptions• Standard Manual Operation• Optional Fully-Automated Operationwith Marathon ® Cryopump Controller• Displex ® and Whisper ® Technology Standard Scope of Supply • CP-12 Cryopump • HC-8E4 or F-70L/H Compressor • 10’ Flexible Gas Lines • 10’ Cold Head Cable •Tool KitPerformance Specifications Performance Specifications Available Configurations• ISO 400, CVC 10” or Wire Seal FlangeOptions• Standard Manual Operation• Optional Fully-Automated Operationwith Marathon ® Cryopump Controller• Displex ® and Whisper ® Technology Standard Scope of Supply • CP-16 Cryopump • HC-8E4 or F-70L/H Compressor • 10’ Flexible Gas Lines • 10’ Cold Head Cable • Tool Kit Available Configurations• ISO 500, ANSI 20” or Wire SealFlange Options• Standard Manual Operation• Optional Fully-Automated Operationwith Marathon ® Cryopump Controller• Displex ® and Whisper ® Technology Standard Scope of Supply • CP-20 Cryopump • F-70L/H Compressor • 10’ Flexible Gas Lines • 10’ Cold Head Cable •Tool KitPerformance Specifications Performance Specifications Available Configurations• ICF 253 mm Flange• Standard Fully-Automated Operation• SHI Proprietary Inverter Technology Standard Scope of Supply • KZ-8L Cryopump • CSW-61C/D Compressor • Remote Cryopump Controller with RS-485 Cables • Flexible Gas Lines • Power Cables Available Configurations• ANSI 10” Flange• Standard Fully-Automated Operation• SHI Proprietary Inverter Technology Standard Scope of Supply • KZ-12L Cryopump • CSW-61C/D Compressor • Remote Cryopump Controller with RS-485 Cables • Flexible Gas Lines •Power CablesSHI offers a complete line of necessary interconnect-ing cables for our Marathon ® CP Cryopump Sys-tems. Standard, manual systems include cables that transmit the necessary power from our compressors to the cryopump cold head. Standard length is 10 feet (3 meters) with options to extend up to 66 feet (20 meters). For our fully automatic, MCC-driven systems, additional interconnecting cables are in-cluded to power the cold head, MCC, automatic valves, blanket heater and vacuum and temperature instrumentation. RS-232 cables connect between our optional MCC and the customer’s host computer, PLC or PC.The SI CERA ® Cryopump system includes power cables for the pumps, compressors and controller. In addition, RS-485 cables connect the con-troller to both the pumps and compressors. SI CERA™ system cablescome in a variety of lengths and can be customized to fit the customer’sprocess.SHI offers Temperature I ndicator Kits, de-signed to accurately display and/or com-municate critical cryopump temperatures forour Marathon ® CP Cryopumps. Model 1901Indicator is a single, Model 9302 is dual, andModel 9304 is a four channel temperature in-dicator. All have alarm set points, RS-232 interface and analogoutput (optional on Model 1901). Model 9302 and 9304 Indica-tors additionally have a standard Ethernet interface. Tempera-ture indicators provide the necessary excitation and accuratereadout for our standard temperature diodes and kits comecomplete with 50 foot interconnecting cable(s).The S CERA ® Remote Cryopump Control-ler enables fully automatic operation of SICERA ® Cryopumps using commands from the end user’s host computer and industrystandard cryopump protocol. The controller comes standard with all SICERA®Cryopump systems. An Operation Panel Unit (shown in picture) is available as an option to monitor the status of the cryopumps and compressors, as well as to modify the regen-eration sequence and to obtain key data from the cryopump system.SHI’s MCC enables fully automatic operation of Marathon ® CP Cryopumps. Industry standard cryopump protocol is delivered via RS-232 interface from the customer’s host computer, PLC or Windows-based PC (using optional SHI MCS Software). Automatic operation and regeneration, as well as monitoring of critical system functions, are enabled, resulting in im-proved process times, enhanced efficiency of the user’s process and greatly reduced down-time between production cycles. I n conjunc-tion with the MCC, Marathon ® CP Cryopumps are enhanced with all necessary automatic valves, vacuum and temperature instrumen-tation and blanket heaters to enable safe andefficient automatic operation and regeneration.Tool Kits & Replacement Parts KitsCables Flexible & Superflex Gas LinesMarathon ® Cryopump Controller (MCC)SICERA ® Remote Cryopump Controller Temperature IndicatorsSICERA ® Cryopumps come equipped with flex-ible helium gas lines in 82 feet (25 meter) lengths,while Marathon ® CP Cryopumps come standardwith flexible helium gas lines in lengths from 10feet (3 meters) to 66 feet (20 meters). Gas linesterminate in size 8 female coupling halves forquick connect and disconnect to/from the coldhead and compressor and are also available withone end at 90°.Optional Superflex lines offer superior flexibilityand smaller bend radius without thinning the wall of the hose and of-fer a higher flexing cycle life than standard lines. Superflex lines alsodampen vibration and noise of the helium gas traveling through thelines. All flexible gas lines are pre-charged with clean helium gas.SHI offers a complete line of replacementparts kits that include all of the required partsand assemblies to completely reconditionMarathon ® CP Cryopumps and compres-sors.Tool kits are available from the standardwrench kit (used for connecting couplings)that accompanies new Marathon CP ® sys-tems to more comprehensive kits that in-clude such items as gas charging valves andadditional tools required for performing yourown service on Marathon ® CP Cryopumps and compressors.Contact your local SHI office for details.GLOBAL SERVICE & SUPPORT PROGRAMSAt SHI Cryogenics Group, we realize that our customers are diverse and the markets they serve are demanding and unique. In response, our global service and support network offers responsive and value-added support for our complete range of products. Our factory-trained technicians are located in strategic service centers around the globe and offer 24/7 on-call support, with no machines and no waiting.Our cryopump service offerings are both flexible and cost effective, including:• Product return to regional service depot for service, repair or complete refurbishment• Technical assistance in diagnosing equipment issues via phone or e-mail• Product exchange programs• Customer training programs• Customized service contractsAdditionally, Marathon® CP Cryopumps, can be serviced on-site, in-situ by the cus-tomer or a SHI factory-trained technician, without breaking vacuum or remov-ing the cryopump from the chamber for return or replacement. This uniqueservice option is the result of the high-quality, ultra-reliable Displex®Cryocooler technology found in all Marathon® CP Cryopumps.Displex® Cryocoolers have a long and successful operatinghistory, and feature a pneumatic drive that optimiz-es performance, reliability and main-tainability.Performing in-situ service lowers the total cost of ownership by:• Minimizing the required capital investment in spare parts• Minimizing the “down time” of your system for service or repair• Eliminating the cost of shipping a complete cryopump to a service center• Eliminating labor costs associated with complete disassembly of the cryopump from your systemSICERA® Cryopumps can be returned to one of SHI’s service centers for routine maintenance, service or complete refurbishment. Additional SICERA® pumps and compressors are available as “exchange units.” Simply install the exchange unit and the returned unit will be refurbished and placed “on the shelf” ready for the next exchange.Additionally, our factory-trained service technicians are available for on-site training, scheduled maintenance or emergency visits, offering rapid-response service for mission-critical applications.Whether you have service performed by a qualified service technician, perform in-situ service yourself with readily-ADDITIONAL PRODUCTS FROM SHI CRYOGENICS GROUP In addition to the cryopumps featured in this catalogue, SHI Cryogenics Group designs and manufactures4K and 10K G-M Cryocoolers, Pulse Tubes and other low temperature cooling technology.SHI Cryogenics Group’s 10K Gifford-McMahon Cryocoolers are versatile, orientation-free, closed-cycle systems that feature the same Displex® technology found in the complete line of Marathon® CPCryopumps and MRI coolers, proven the world over with millions of reliable operating hours. Theyhave been recognized as the industry standard since we developed the technology over 40 years ago.Our original pneumatic drive, which limits the number of wear parts in the refrigerator, combined withstate-of-the-art design features, results in superior performance and low maintenance costs. Selectmodels, such as the CH-208 (left), also feature Whisper® technology for quieter operation.SHI’s 10K Cryocoolers have proven reliability in thousands of applications, includingMRI, cryopumping, research and other custom low-temperature applications.SH I CryogenicsGroup’s 4K Gifford-McMahon Cryocoolers arerecognized as the most reliableand versatile systems available in themarketplace. These Cryocoolers featurehigh cooling capacities, compact designs and areorientation-free. Models like the SRDK-408D2 (left) are the standardfor MRI and other superconducting magnets and can be found cooling awide variety of analytical and experimental devices and offer a verycost effective alternative to open-cycle liquid helium systems.SHI’s 4K Pulse Tube Cryocoolers embody leading-edge technologyand provide low vibration, high reliability and low maintenancerequirements. They are uniquely designed with no moving partsinside the coldhead. I n addition, the SRP-062B (right) featuresan optional separated valve unit to further reduce vibration, enableoperation in higher magnetic fields and ease maintenance requirements. SHI PulseTube Cryocoolers provide a stable low-temperature solution for sensitive measurementand analytical applications.For additional literature and information regarding 10K Cryocooler, 4K G-M and PulseTube Cryocooler designs, please contact your local SHI Cryogenics Group sales office.11For Information in:AsiaSumitomo Heavy Industries, Ltd.ThinkPark TowerCryogenics Division, Sales Department 1-1, Osaki 2-Chome, Shinagawa-Ku Tokyo 141-6025, Japan Phone: +81-3-6737-2550Fax: +81-3-6866-5114E-mail:***********.jpCryogenics Division, Service Department 2-1-1, Yato-cho, Nishitokyo-city Tokyo 188-8585, Japan Phone: +81-42-468-4265Fax: +81-42-468-4254E-mail:*******************.jpUnited StatesSumitomo (SHI) Cryogenics of America, Inc.1833 Vultee Street Allentown, PA 18103Phone: +1 610-791-6700Fax: +1 610-791-0440E-mail:***********************EuropeSumitomo (SHI) Cryogenics of Europe, Ltd.3 Hamilton Close, Houndmills Industrial Estate Basingstoke, Hampshire RG21 6YT United KingdomPhone: +44 (0) 1256 853333Fax: +44 (0) 1256 471507E-mail:************************.ukSumitomo (SHI) Cryogenics Shanghai, Ltd.Building 15Lane 333 Zhujian Road Minhang DistrictShanghai 201107, P .R. China Phone: +86-21-5486-6318Fax: +86-21-5486-0065E-mail:***********************.jpSumitomo (SHI) Cryogenics of America, Inc.1700 Wyatt Drive Suite 13Santa Clara, CA 95054Phone: +1 408-645-3346Fax: +1 408-736-7325Sumitomo (SHI) Cryogenics of Europe, GmbH Daimlerweg 5aDarmstadt D-64293, Germany Phone: +49 (0) 6151 860 610Fax: +49 (0) 6151 800 252E-mail:***********************Sumitomo (SHI) Cryogenics Korea Co., Ltd.3F , 280-3, Saneop-ro155beon-gil, Gweonseon-GuSuwon-City, Gyeonggi-Do, South Korea Phone: +82-31-278-3050Fax: +82-31-278-3053E-mail:******************.jpSumitomo (SHI) Cryogenics T aiwan Co., Ltd.4th Floor, No. 3Lane 216, Gongyuan Rd.Hsinchu City 300, Taiwan ROC Phone: +886 3 561 2557Fax: +886 3 562 3400Sumitomo (SHI) Cryogenics of America, Inc.1500-C Higgins RoadElk Grove Village, IL 60007Phone: +1 847-290-5801Fax: +1 847-290-1984World Wide Web: © SHI Cryogenics Group 2/16。
pen needle生产标准
pen needle生产标准英文回答:Pen Needle Production Standards.Pen needles are small, thin needles used to inject medication into the body. They are typically used by people with diabetes to inject insulin, but they can also be used to inject other medications such as growth hormone or blood thinners.Pen needles are made of a variety of materials, including stainless steel, plastic, and rubber. The needles are typically sharpened to a fine point to reduce pain and discomfort during injection.Pen needles are manufactured according to a variety of standards, including ISO 11608-1 and ISO 11608-2. These standards specify the dimensions, materials, and performance requirements for pen needles.The following are some of the key requirements for pen needles:Dimensions: The length of the needle should be between 4mm and 13mm. The outer diameter of the needle should be between 0.25mm and 0.33mm.Materials: The needle should be made of stainlesssteel or another suitable material that is biocompatible and resistant to corrosion.Performance: The needle should be sharp and able to penetrate the skin without causing excessive pain or discomfort. The needle should also be able to withstand multiple injections without breaking or becoming damaged.Pen needles are an important part of diabetes management and other medical treatments. By meeting the requirements of the relevant standards, manufacturers can ensure that pen needles are safe, effective, and reliable.中文回答:钢笔针生产标准。
Axil Scientific Tryptone Bacteria Culture Media 40
SECTION 1 – IDENTIFICATIONProduct Identifier:TryptoneBacteria Culture MediaCatalogue Number:4040Other means of identification: Not availableRecommended use of the chemical and restrictions on use:Recommended for preparing media where enzymatic hydrolyzed casein is desired.For R&D use only. Not for pharmaceutical, household or other uses.Supplier Information:Axil Scientific Pte Ltd Apical Scientific Sdn Bhd41 Science Park Road No 7-1 to 7-4 Jalan SP 2/7#04-08 The Gemini Taman Serdang Perdana, Seksyen 2Singapore Science Park II Seri Kembangan 43300Singapore 117610 Selangor Darul Ehsan, MalaysiaTel: +65 6775 7318 Tel: +603 8943 3252Fax: +65 6775 7211 Fax: +603 8943 3243Email: Email:Emergency phone number:Monday – Friday, 8:00 a.m. to 6:00 p.m.+65 6775 7318 (Singapore)+603 8943 3252 (Malaysia)SECTION 2 – HAZARDS IDENTIFICATIONGHS ClassificationNot a dangerous substance or mixture according to the Globally Harmonised System (GHS). Other Hazards - NoneSECTION 3 – COMPOSITION/ INFORMATION ON INGREDIENTSChemical Identity:TryptoneCAS No.:73049-73-7SECTION 4 – FIRST-AID MEASURESEye ContactFlush eyes with water as a precaution.Skin ContactImmediately wash skin thoroughly with soap and copious amounts of water.InhalationIngestionNever give anything by mouth to an unconscious person. Rinse mouth with water.Most important symptoms and effects, both acute and delayedTo the best of our knowledge, the chemical, physical, and toxicological properties have not been thoroughly investigated.Indication of immediate medical attention and special treatment neededData not available.SECTION 5 – FIRE-FIGHTING MEASURESExtinguishing MediaUse water spray, dry chemical powder, carbon dioxide or alcohol-resistant foam.Special Exposure HazardsCarbon oxides.Special Fire-fighting ProceduresWear self-contained breathing apparatus and protective clothing to prevent contact with skin and eyes. SECTION 6 – ACCIDENTAL RELEASE MEASURESPersonal PrecautionsPrevent skin/eye contact. Use personal protective equipment. Avoid dust formation. Ensure adequate ventilation. Avoid breathing dust.Environmental PrecautionsDo not allow material into sewers and drainage systems.Methods for Cleaning UpClean up spills immediately, observing precautions in the safety data sheet and label. Minimize dust generation. Dispose into a chemical waste container.SECTION 7 – HANDLING AND STORAGEPrecautions for safe handlingPrevent skin/eye contact. Use personal protective equipment. Avoid dust formation. Ensure adequate ventilation. Avoid breathing dust. Wash thoroughly after handling. Remove contaminated clothing and wash before reuse.Conditions for safe storage, including any incompatibilitiesStore in tightly closed container in a cool, dry and well-ventilated area.SECTION 8 – EXPOSURE CONTROLS/ PERSONAL PROTECTIONOccupational Exposure LimitsWe are not aware of any national exposure limit.Appropriate engineering controlsHandle in accordance with good industrial hygiene and safety practice.Eye/ Face ProtectionUse equipment for eye protection tested and approved under appropriate government standards such as NIOSH (US) or EN 166(EU).Skin/ Hand ProtectionHandle with gloves. Gloves must be inspected prior to use. Use proper glove removal technique (without touching glove's outer surface) to avoid skin contact with this product. Dispose of contaminated gloves after use in accordance with applicable laws and good laboratory practices. Wash and dry hands.The selected protective gloves have to satisfy the specifications of EU Directive 89/686/EEC and the standard EN 374 derived from it.Body protectionChoose body protection in relation to its type, to the concentration and amount of dangerous substances, and to the specific work-place. The type of protective equipment must be selected according to the concentration and amount of the dangerous substance at the specific workplace.Respiratory ProtectionRespiratory protection is not required. Where protection from nuisance levels of dusts are desired, use type N95 (US) or type P1 (EN 143) dust masks. Use respirators and components tested and approved under appropriate government standards such as NIOSH (US) or CEN (EU).SECTION 9 – PHYSICAL AND CHEMICAL PROPERTIESa) Appearance Beige, fine powderb) Odour Not availablec) Odour Threshold Not availabled) pH (2%; H₂O, 25°C) 6.5 – 7.5e) Melting/freezing point Not availablef) Initial boiling point and Not availableboiling rangeg) Flash point Not availableh) Evaporation rate Not availablei) Flammability (solid, gas) Not availablej) Upper/lower Not availableflammability orexplosive limitsk) Vapour pressure (mm Hg) Not availablel) Vapour density Not availablem) Relative density Not available n) Solubility (ies) Not available o) Partition coefficient: Not available n-octanol/waterp) Autoignition temperature Not available q) Decomposition temperature Not available r) Viscosity Not available SECTION 10 – STABILITY AND REACTIVITYReactivityData not available.Chemical stabilityStable.Possibility of hazardous reactionsData not available.Conditions to avoidData not available.Incompatible materialStrong oxidizing agents.Hazardous decomposition productsData not available.SECTION 11 – TOXICOLOGICAL INFORMATION Acute toxicityLD50 (oral): 2000 mg/kg [Rat]Skin corrosion/irritationData not available.Serious eye damage/eye irritationData not available.Respiratory or skin sensitizationData not available.Germ cell mutagenicityData not available.CarcinogenicityIARC: No component of this product present at levels greater than or equal to 0.1% is identified as probable, possible or confirmed human carcinogen by IARC.Reproductive toxicityData not available.Specific target organ toxicity – single exposureData not available.Specific target organ toxicity – repeated exposureData not available.Aspiration hazardData not available.Other informationRTECS: Data not availableSECTION 12 – ECOLOGICAL INFORMATIONToxicityData not available.Persistence and degradabilityData not available.Bioaccumulative potentialData not available.Mobility in soilData not available.Other adverse effectData not available.SECTION 13 – DISPOSAL CONSIDERATIONSProductDissolve or mix the material with a combustible solvent and burn in a chemical incinerator equipped with an afterburner and scrubber. Offer surplus and non-recyclable solutions to a licensed disposal company. Contaminated packagingDispose off as unused product.SECTION 14 – TRANSPORT INFORMATIONUN NumberADR/RID: - IMDG: - IATA-DGR: -IMDG: Not dangerous goodsIATA-DGR: Not dangerous goodsTransport Hazard Class(es)ADR/RID: - IMDG: - IATA-DGR: -Packing GroupADR/RID: - IMDG: - IATA-DGR: -Environmental HazardsADR/RID: no IMDG: marine pollutant: no IATA-DGR: noSpecial Precaution for UsersData not availableSECTION 15 – REGULATORY INFORMATIONSafety, health and environmental regulations/legislation specific for the substance or mixtureData not availableSECTION 16 – OTHER INFORMATIONDate of Issue: JULY 11, 2008 Date of Revision: MAY 07, 2017The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. The information in this document is based on the present state of our knowledge and is applicable to the product with regard to appropriate safety precautions. Axil Scientific Pte Ltd shall not be held liable for any damage resulting from handling or from contact with the above product.。
生物制品中 唾液酸残留标准
生物制品中唾液酸残留标准英文回答:Sialic acid is a naturally occurring compound found in many biological products, including human saliva. It is commonly used as a marker for evaluating the quality and safety of these products. The residual level of sialic acid is an important parameter that needs to be monitored and controlled to ensure product quality.In the field of biopharmaceuticals, there are specific standards and regulations set by regulatory authorities such as the U.S. Food and Drug Administration (FDA) and the European Medicines Agency (EMA) for the allowable residual level of sialic acid in biological products. These standards vary depending on the type of product and its intended use.For example, in the case of therapeutic proteins derived from mammalian cell cultures, the FDA hasestablished a limit of 10 µg of sialic acid per mg of protein as an acceptable level. This limit ensures that the product is free from excessive sialic acid, which can potentially affect its safety and efficacy.To comply with these standards, biopharmaceutical manufacturers employ various purification and analytical techniques to remove or reduce the level of sialic acid in their products. These techniques include chromatography, filtration, and enzymatic treatments. By implementing these processes, manufacturers can effectively control the residual level of sialic acid and ensure product quality.In addition to regulatory standards, individual companies may also have their own internal specifications for sialic acid residues in their products. These specifications are often more stringent than regulatory requirements and are based on the company's own research and development efforts.中文回答:唾液酸是一种自然存在的化合物,存在于许多生物制品中,包括人类的唾液中。
喷胶棉 专业英语
Polyester wadding, commonly known as flocking sponge or spray-bonded cotton, is an essential material in various industries due to its unique properties and versatility. This comprehensive analysis delves into the multifaceted aspects that define high-quality and high-standard polyester wadding, emphasizing its production processes, material characteristics, applications, and environmental implications.**Production Processes: Ensuring Optimal Quality**High-quality polyester wadding begins with stringent control over the manufacturing process. Key stages include:1. **Fiber selection**: The use of premium, virgin polyester fibers ensures consistent quality and performance. These fibers exhibit high tensile strength, low elongation, and excellent resilience, providing the foundation for a durable, resilient, and uniform wadding product.2. **Opening and blending**: Fibers are opened and blended meticulously to ensure homogeneity and eliminate impurities. Advanced machinery, such as rotor cards and air-jet systems, facilitate efficient fiber separation and mixing, contributing to a consistent final product.3. **Spray bonding**: The unique spray-bonding process involves atomizinga low-melt adhesive onto the web of fibers, which is then thermally bonded. This method allows for precise control over loft, density, and thickness, resulting in customized wadding with tailored properties. High-quality adhesives and accurate application techniques prevent over-bonding, preserving the material's softness, breathability, and insulation properties.4. **Calendering and finishing**: Post-bonding, the wadding undergoes calendering to achieve the desired thickness and smoothness. Advanced calendaring machines maintain consistent pressure and temperature, ensuring uniformity across the width and length of the fabric. Finishing treatments may include anti-static agents, flame retardants, or hydrophobic coatings, depending on the intended application.**Material Characteristics: Meeting Stringent Standards**High-standard polyester wadding exhibits several key characteristics that set it apart from lower-quality alternatives:1. **Loft and compressibility**: A hallmark of high-quality wadding is its ability to maintain a consistent, lofty appearance while exhibiting excellent compressibility. This balance provides superior cushioning, insulation, and shock absorption without compromising bulkiness.2. **Air permeability and breathability**: Open-cell structures and optimized bonding patterns allow for efficient air circulation, preventing moisture buildup and promoting thermal regulation. This attribute is particularly crucial in applications such as bedding, upholstery, and outdoor gear.3. **Uniformity and stability**: High-standard wadding exhibits minimal variation in weight, thickness, and density across the fabric, ensuring consistent performance and aesthetics. It also demonstrates excellent dimensional stability, resisting shrinkage or distortion during laundering or prolonged use.4. **Durability and resilience**: The use of high-quality polyester fibers and optimal bonding techniques results in a product that withstands repeated compression, abrasion, and mechanical stress without losing its original properties. This durability is vital in applications subject to frequent handling or heavy usage.**Applications: Versatility and Performance**High-quality polyester wadding finds diverse applications across various sectors, where its unique properties deliver exceptional performance:1. **Textiles and apparel**: As a filling material in jackets, sleeping bags, and pillows, wadding provides warmth, comfort, and lightweight insulation. In upholstery and bedding, it offers excellent support, breathability, and hypoallergenic properties.2. **Automotive**: Used in car seats, headrests, and sound insulation, wadding enhances passenger comfort, reduces noise levels, and contributes toenergy absorption in case of collisions.3. **Packaging**: High-loft wadding provides effective cushioning and protection for fragile goods during transportation and storage, reducing damage and product returns.4. **Construction and insulation**: In building materials, wadding serves as an efficient thermal and acoustic insulator, improving energy efficiency and indoor comfort.**Environmental Implications: Sustainability and Responsibility**In today's environmentally conscious landscape, high-standard polyester wadding manufacturers are increasingly adopting sustainable practices:1. **Recycled content**: Utilizing recycled polyester fibers reduces waste, conserves resources, and lowers the carbon footprint associated with raw material extraction and processing.2. **Energy-efficient production**: Implementing energy-saving technologies and processes, such as heat recovery systems and optimized machine settings, minimizes energy consumption during manufacturing.3. **End-of-life considerations**: Some high-quality wadding products can be recycled or repurposed at the end of their useful life, further contributing to the circular economy.4. **Compliance with eco-labels and regulations**: Meeting stringent environmental standards, such as bluesign®, OEKO-TEX®, and REACH, ensures the absence of harmful substances and demonstrates commitment to responsible production.In conclusion, high-quality and high-standard polyester wadding is the result of meticulous production processes, exceptional material characteristics, versatile applications, and a commitment to environmental responsibility. By continuously refining these aspects, manufacturers can cater to the evolving needs of various industries while contributing to a more sustainable future.。
2023年版最新医疗废品分门别类指南英文版
2023年版最新医疗废品分门别类指南英文版2023 Latest Medical Waste Sorting GuideIn 2023, proper sorting and disposal of medical waste is crucial for maintaining a safe and healthy environment. This guide provides information on how to categorize different types of medical waste to ensure proper handling and disposal.Types of Medical Waste1. Infectious Waste: This includes items contaminated with blood or other bodily fluids, such as gloves, bandages, and needles.2. Hazardous Waste: Chemicals, pharmaceuticals, and other materials that pose a risk to human health or the environment fall under this category.3. Sharps: Needles, syringes, and other sharp objects used in medical procedures should be separated and disposed of in designated containers.4. Radioactive Waste: Materials contaminated with radioactive substances, such as used equipment or materials from nuclear medicine procedures.5. Pathological Waste: Tissues, organs, and body parts removed during surgery or autopsy are considered pathological waste.6. Pharmaceutical Waste: Expired or unused medications, as well as empty drug containers, should be disposed of properly to prevent contamination.Sorting Guidelines- Color Coding: Use color-coded containers for different types of medical waste to easily identify and separate them.- Labeling: Clearly label each container with the type of waste it contains to avoid confusion and ensure proper disposal.- Segregation: Separate different types of waste at the point of generation to prevent cross-contamination and facilitate recycling.- Training: Educate healthcare workers on the proper sorting and disposal of medical waste to ensure compliance with regulations.- Disposal Methods: Follow local regulations and guidelines for the safe disposal of medical waste, including treatment and disposal facilities.Benefits of Proper Sorting- Reduced Risk of Infections: Proper handling of medical waste minimizes the risk of healthcare-associated infections and protects both patients and healthcare workers.- Environmental Protection: Correct disposal of medical waste prevents pollution of land, water, and air, preserving the environment for future generations.- Compliance: Adhering to waste management regulations and guidelines ensures legal compliance and avoids fines or penalties.- Resource Conservation: Recycling and proper disposal of medical waste contribute to resource conservation and sustainability.By following the latest medical waste sorting guidelines in 2023, healthcare facilities can promote a safe and sustainable environment for all. Remember, proper sorting and disposal of medical waste are everyone's responsibility.。
ConsistencyandReproducibilityofDNAIsolation…
Consistency and Reproducibility of DNA Isolation From Whole Blood Using 96-Well DNA Binding Plate and Liquid-Handling Robot for Samples Under Different Storage ConditionsGalina Fomovska and Daniel Kim, Pall Life Sciences, Port Washington, NYAn optimized protocol for automated DNA isolation using a 96-well DNA binding plate and a liquid-handling robot was developed. Vacuum filtration was used for all liquid evacuation steps.The protocol was verified for DNA isolation from blood under different storage conditions with multi-well plates from two manufacturers.Plate performance was monitored by measuring DNA yield and dsDNA purity in each well.DNA yield (µg/well) from100 µL of blood was measured by OD260and Pico Green u assays.Percent DNA recovery was calculated with white blood cell (WBC) concentration from each blood sample.DNA quality was assessed by an A260/280 ratio for each well and gel electrophoresis run for every other well in the plate.Intra-plate (well-to-well) and inter-plate (plate-to-plate) consistency was evaluated by comparing averages, standard deviation (SD), and CV characteristics of each plate. Protocol outcomes were found dependable on blood storage conditions.Fresh blood produced the best gDNA yield at 3.7 µg per well and 86% total predicted recovery from 100 µL of blood.5-hour lysate blood produced poor gDNA yield at 2.7 µg per well and 63% of total predicted recovery.Freeze-thaw blood demonstrated a decrease in WBC number and DNA yield compared to fresh samples, 2.3 µg versus 3.7 µg per well, respectively.The purity of gDNA for all conditions was in the range of industry expectations—A260/280 ratio of 1.7-1.9 from fresh blood and 1.6-1.8 from 5-hour lysate and freeze-thaw blood.Inter-plate performance was similar for all plates used in the study, varying in the range of one SD for DNA yield and DNA purity characteristics.There are a number of commercial kits consisting of a DNA binding plate and ready-made buffers for DNA isolation from blood. Pall Life Sciences has recently developed a DNA binding plate for isolation of DNA from a variety of sources. It is necessary to demonstrate that Pall’s AcroPrep™ Advance DNA binding plate is capable of interchangeable use with solution kits from other manufacturers that employ glass fiber medium for gDNA isolation and purification. Previous studies proved the viability of processing the Pall filter plate with centrifugation and this study intended that vacuum filtration could be employed when using an automated processing system, such as the Eppendorf epMotion u5075.ObjectivesOptimize the automation protocol enabling consistent and reproducible gDNA isolation from human blood using vacuum filtration for all liquid evacuation steps. Demonstrate the robustness of the protocol using an automated system and plates from two different manufacturers in conjunction with commercially available buffers.I. Materials RequiredPall AcroPrep Advance DNA Binding Plate (PN 8132)Eppendorf DWP (0030 522.109, 2000 μL/well)Corning UV/Vis sample collection plate (capacity > 250 μL/well)Eppendorf Thermo adapter DWP 96 (960002391)Eppendorf EpMotion 5075 vacuumE-Gel u Agarose Gel Electrophoresis System (Invitrogen)E-Gel 48 1% Agarose gels (Invitrogen G8008-01)Quant-iT u Pico Green dsDNA Assay Kit (Invitrogen P7589)Schematic of epMotion 5075 System Customized for DNA Isolation ProtocolII. Solutions RequiredAL Lysis Buffer (Qiagen PN 19075 )Proteinase K (20 mg/mL, Qiagen PN 19131)Wash Buffers AW1 and AW2 (Qiagen PN 19081 and PN 19072, both required)Elution Buffer (Qiagen PN 19077)100% EtOHIII. DNA Isolation and Purification ProtocolThe automation protocol consists of four steps, at each step whole blood or DNAsamples are transferred from one plate to another.1.Automation set-up – All solutions, buffers, and disposables are in place as perschematics. Blood samples are loaded into sample prep 2 mL plate.2.Cell lysis and DNA isolation – Blood samples are lysed, DNA is incubated withchaotropic salt buffer in 2 mL sample prep plate.3.DNA purification – DNA bound to the glass fiber media on DNA binding plate,impurities washed out using two subsequential wash buffers.4.DNA elution – DNA is released from the DNA binding plate into collection platewith TE buffer.DNA EvaluationDNA yield, µg/well, was measured by OD260and Pico Green assays. PercentDNA recovery was calculated from WBC concentration in each blood sample.DNA quality was assessed by A260/280 ratio for each well and gel electrophoresisrun for every other well in the plate.The protocol robustness has been demonstrated using multiple plates fromtwo manufacturers by comparing average yield, SD and CV (%) characteristicsof each plate. Six plates from Pall and three plates from Manufacturer Q wereused in the study.DNA Isolation From Blood UnderDifferent Storage ConditionsAn optimized protocol for automated DNA isolation using Pall’s AcroPrep AdvanceDNA binding plates and a liquid-handling robot was developed. Vacuum filtrationwas used for all liquid evacuation steps.Results presented in T able 1 are representative of Pall DNA binding plate performancewith blood from the same donor, but under different storage conditions: freshEDTA blood, freeze-thaw blood, and lysate that was stored for 5 hours before DNAisolation. 24 wells of the filter plate were used for each condition.Table 1Performance of Pall DNA Binding Plate with Blood Under Different Storage ConditionsFresh Freeze/Thaw 5 Hour LysateDNA Available (µg) 4.3 2.5 4.3Purity (A260/A280) 1.8 +/- 0.1 1.7 +/- 0.1 1.7 +/- 0.1Total Yield (µg) 3.7 +/- 0.5 2.3 +/- 0.5 2.7 +/- 0.7% Recovery869263CV (%) Recovery142226The performance of the plate was found dependable on blood sample condition. Freshblood produced the best gDNA yield at 3.7 µg per well/86% total recovery. Freeze/thawblood demonstrated significant decrease in WBC count; although the gDNA yield was92%, the amount of recovered DNA was only at 2.3 µg per well. 5-hr lysate blood producedpoor gDNA yield at 2.7 µg per well/63% of total recovery. The purity of gDNA for allconditions was in the range of industry expectations – 1.7-1.9 from fresh blood; 1.6-1.8 from5-hr, lysate, and freeze thaw blood.In Figure 1, the distribution of the DNA yield data from blood samples underdifferent storage conditions is presented. DNA yield, µg/well, from 100 µL bloodin each well was measured by OD260(blue dots) and Pico Green (green dots)assays. Available DNA (red triangles) was calculated based on WBC amount in100 µL blood assuming 7 pg DNA per cell.Figure 1DNA Yield (µg per well) Depending on Different Blood Sample ConditionsThe best DNA yield and well-to-well consistency of 14% CV was achieved with a freshblood sample; freeze-thaw and 5-hr lysate blood samples resulted in lower gDNA yieldalong with higher well-to-well variability in the range of 21-26% CV.In Figure 2, the distribution of the DNA purity data from blood samples underdifferent storage conditions is presented. DNA quality in each well was assessedby A260/280 ratio for each well (purple dots) and gel electrophoresis run for everyother well in the plate (data not shown).Figure 2Purity of DNA (A260/280 ratio) Depending on Different Blood Sample ConditionsThe quality of gDNA for all blood sample conditions was found in the range of industryexpectations, 1.7-1.9 for samples of fresh blood, 1.6-1.8 for freeze-thaw and 5-hr lysateblood. Well-to-well consistency was the best for samples of fresh blood.Protocol Robustness: Intra-/Inter-Plate ConsistencyProtocol robustness has been demonstrated using multiple plates from twomanufacturers by comparing averages, SD and CV (%) of DNA yield of each plate.Fresh EDTA blood collected from the same donor on the days of the test was used.In T able 2, the performance of six plates from Pall and three plates from ManufacturerQ are summarized in terms of DNA quality assessed as A260/280 ratio.Table 2DNA Purity, Intra- and Inter-Plate Consistency: Plates From Two ManufacturersOD260/280ratio SD CV (%)Pall Plate 1 1.760.05 2.71Plate 2 1.830.07 3.86Plate 3 1.830.06 3.07Plate 4 1.760.11 6.44Plate 5 1.760.06 3.51Plate 6 1.720.03 1.99Average for all Pall 1.780.06 3.60OD260/280ratio SD CV (%)Manufacturer Q Plate 1 1.780.06 3.46Plate 2 1.740.08 4.79Plate 3 1.740.04 2.45Average for all Manufacturer Q 1.750.06 3.57Inter-plate consistency was similar for six Pall plates and three Manufacturer Q plates,plate averages varied in the range of one SD. Intra-plate consistency, CV (%), was ~6% orless for all plates, better than variability commonly expected for the application.In Figure 3 below, the performance of six plates from Pall and three plates fromManufacturer Q is summarized. Each green column represents a plate performanceas the average of DNA yield as percent of available DNA from 96 wells (with theexception of plate #5 represented by 24 wells). Orange dots show CV% for a plate,error bars show one SD range.Figure 3DNA Yield, Intra- and Inter-Plate Consistency, Plates From Two ManufacturersIn Figure 4, the distribution of individual well performance as DNA yield presentedas percent of available DNA, is shown for six Pall DNA binding plates; dsDNA wasmeasured by Pico Green assay while available DNA was calculated based on theamount of WBC per well assuming 7 pg DNA per cell.Figure 496 Data Points for All Plates with an Exception of Plate #5 Presented by 24 Data PointsThe plate averages varied in the range of 71-86% of DNA yield; 3-SD distributionof individual well data points was between 50-100%.1.An optimized protocol for automated DNA isolation using Pall’s AcroPrepAdvance DNA binding plates and a liquid-handling robot was developed usingvacuum for all liquid evacuation steps.2.Robustness of the procotol was demonstrated using plates from two manufacturers:Inter-plate consistency, DNA yield, and purity was similar for six Pall platesand three Manufacturer Q plates varying in the range of one SD.Intra-plate consistency, CV (%), was 11% or less for DNA yield and ~6% forDNA purity as measured A260/280 ratio, better than commonly expected forthe application.3.Protocol was verified for blood samples under different blood storage conditions:Fresh blood produced the best gDNA yield at 3.7 µg per well, 86% totalrecovery; freeze-thaw and 5-hr lysate blood resulted in decreased amount ofrecovered DNA.The purity of gDNA for all blood storage conditions was in the range ofindustry expectations—1.7-1.9 from fresh blood, 1.6-1.8 from stored blood.DNAYield,%ofAvailableDNAYield,%ofAvailableDNA Yield, % of AvailableCV(%)CV (%)OD26/28RatioFreshBlood Columns5-HrLysateFreeze/Thaw ColumnsColumnsDNAYield,µg/wellBlood Columns LysateThaw260Pico Green ValuesColumnsColumnsClamp1000 µL50 µLPipettes50 µL Tips 1000 µL Tips Shaker/HeatingThermo AdapterDWP 96 (960002391)2 mL Proteinase K Tub1-5Tub6-7VacuumWaste Bucket Sample Prep Plate Corning UV/Vis96-well plate2 mL DNABinding PlateVacuum HolderInter-plate consistency assessedby comparing averages of DNAyield for each plate was similarfor six Pall plates and threeManufacturer Q plates, varyingin the range of one SD.Intra-plate consistency, CV (%),calculated for each plate was11% or less, which is betterthan variability commonlyexpected for the application.。
彩盒亚胶英语表达
彩盒亚胶英语表达Title: The Role of Aqueous Varnish in Color Box PackagingIn the world of packaging, especially for consumer products, the aesthetic appeal and durability of packaging materials play a crucial role. One such material that enhances both appearance and functionality is aqueous varnish, commonly used in color box packaging.Aqueous varnish is a water-based coating applied to printed surfaces to improve their visual and physical properties. Unlike solvent-based varnishes, aqueous varnish is environmentally friendly and has lower levels of volatile organic compounds (VOCs), making it a more sustainable choice.One of the primary advantages of aqueous varnish is its ability to enhance the visual appeal of color boxes. By providing a glossy or matte finish, it cansignificantly impact the final look of the packaging.A glossy finish can make colors appear more vibrant and attractive, while a matte finish offers a sophisticated and elegant touch. This visual enhancement helps color boxes stand out on store shelves and capture the attention of consumers.In addition to its aesthetic benefits, aqueous varnish also improves the durability of color boxes. The varnish forms a protective layer over the printed surface, which can resist scratches, smudges, and other forms of damage. This added protection ensures that the color boxes maintain their quality and appearance throughout the supply chain and while in use by the consumer.Another important feature of aqueous varnish is its fast-drying capability. This property allows for efficient production processes and reduces the risk of smudging or damage during handling and packaging. The quick drying time also contributes to a faster turnaround in production, which is beneficial formeeting tight deadlines and fulfilling orders promptly.Furthermore, aqueous varnish is versatile and can be applied to various types of paper and board materials used in color box manufacturing. This adaptability makes it a suitable choice for a wide range of products, from food packaging to electronics and cosmetics.Despite its many benefits, the application of aqueous varnish does require careful consideration of factors such as the type of paper used and the desired finish. Proper application techniques and equipment are essential to achieve the best results and ensure that the varnish performs effectively.In conclusion, aqueous varnish plays a vital role in the packaging industry, particularly for color boxes. Its ability to enhance visual appeal, provide durability, and support efficient production processes makes it a valuable material. As packaging continues to evolve, the use of environmentally friendly andeffective coatings like aqueous varnish will remain important for creating high-quality and attractive packaging solutions.。
- 1、下载文档前请自行甄别文档内容的完整性,平台不提供额外的编辑、内容补充、找答案等附加服务。
- 2、"仅部分预览"的文档,不可在线预览部分如存在完整性等问题,可反馈申请退款(可完整预览的文档不适用该条件!)。
- 3、如文档侵犯您的权益,请联系客服反馈,我们会尽快为您处理(人工客服工作时间:9:00-18:30)。
908737 - Post Closing in Material LedgerSymptomYou would like some clarification on how the system works in step 'Post Closing' for costing run in transaction CKMLCP.Other TermsCNACT, Post closing, CKMLCP, FI accounts, Posting LogicReason and PrerequisitesThe material ledger and is active and material has price determination 3SolutionIf you do not fully understand the 'Post closing' step in CKMLCP or how the FI accounts are posted when running this step, this SAP note should help you for the analysis. It provides explanations for how the values arose in the different FI accounts.During the periodic transactions price/exchange rate differences are recorded under different transaction keys:PRD ( ) Price differences from purchase orderPRD (PRF) Price differences from goods receipt with external value from order settlement.KDM Exchange rate differences from goods receipts, with external value from order settlement.AUM Price differences and exchange rate differences from stock transferAKO Price differences from stock transfer consignment stock to ownAt the end of the period, by running transaction CKMLCP, the Material Ledger settles the differences to the different receivers according to the actual movements of the period.Transaction CKM3 shows, how the differences were distributed to the different receivers.By executing the CKMLCP step 'Closing Entries' these results are posted in FI.The Material Ledger postings use own transaction keys for price/exchange rate difference acccounts:PRY Price differences from same (upper) levelPRV Price differences from lower levelKDM Exchange rate differences from same (upper) levelKDV Exchange rate differences from lower levelPRM Differences of cancelled WIP (Materials)PRA Differences of cancelled WIP (Activities)For the offset postings the following transaction keys are used:BSX Inventory postingLKW Accruals and defer.acct.(Material ledger)UMB Gain/loss from revaluationCOC Revaluation of other consumablesWPM WIP from Price Differences (Material)WPA WIP from Price Differences (Int. Acty)GBB (AUI) Cost Center Crediting from ActivitiesThe different postings that occur in Material Ledger are:1. ROLL UP OF PRICE/EXCHANGE RATE DIFFERENCES* from input material to output material.INPUT MATERIAL OUTPUT MATERIALPRY PRV -> PRVKDM KDV -> KDV* from input activity/business process to output material.COST CENTER OUTPUT MATERIALGBB(AUI) -> PRV* from WIP input material to output material.INPUT MATERIAL OUTPUT MATERIALPRM -> PRV* from WIP input activity/business process to output material.INPUT ACTIVITY/BP OUTPUT MATERIALPRA -> PRV2. REVALUATION OF ENDING INVENTORY* Revaluation of Ending Inventory (flag 'Revaluate material'set):Price/exchange rate differences are posted to Inventory AccountBSX. Price control is changed from 'S' to 'V' because inventoryis now valuated at actual price.PRY PRV KDM KDV -> BSX* No revaluation of Ending Inventory (flag 'Revaluate material notset): Price/exchange rate differences are posted to stastisticalaccount LKW. Price control is not changed, because inventoryremains valuated at standard price.PRY PRV KDM KDV -> LKWThe ending inventory posting is reversed immediately in the next period. For this inverse posting a separate accounting document is created with the first day of following period as posting date.So in the next period the inventory continues to be valuated at standard price (until closing of next period).If the standard price was changed in next period, the inverse posting includes a posting to revaluation account (UMB) to clear the revaluation from price change.Postings for beginning of next period:* Revaluation of Ending InventoryBSX -> PRY PRV KDM KDV UMB* No revaluation of Ending InventoryLKW -> PRY PRV KDM KDV UMB3. REVALUATION OF CONSUMPTION* Revaluation of Original Account:Price/exchange rate differences are posted to the same consumption account that was debited by the original goods issue. ( Optionally the CO account assignment of the original goods issue can berevaluated too.)PRY PRV KDM KDV -> Original account* Revaluation of Collective Account:Price/exchange rate differences are posted to a collective accountCOC.PRY PRV KDM KDV -> COC4. REVALUATION OF WORK IN PROCESS (WIP)* Build-up of new WIP (material):PRY PRV KDM KDV -> WPM* Build-up of new WIP (activity/business process):GBB(AUI) -> WPA* Reduction of old WIP (material):WPM -> PRM* Reduction of old WIP (activity/business process):WPA -> PRA________________________________________________________________________Valid releasesSoftware Component Releasefrom toSAP_APPL SAP Application46C - 500________________________________________________________________________Reference to related Notes。