Trioxsalen_3902-71-4_DataSheet_MedChemExpress
HSF1A_DataSheet_MedChemExpress
Inhibitors, Agonists, Screening Libraries Data SheetBIOLOGICAL ACTIVITY:HSF1A is a cell–permeable activator of heat shock transcription factor 1 (HSF1).IC50 & Target: HSF1[1]In Vitro: HSF1A protects cells from stress–induced apoptosis, binds TRiC subunits and inhibits TRiC activity without perturbation of ATP hydrolysis. Genetic inactivation or depletion of the TRiC complex results in human HSF1 activation and HSF1A inhibits the direct interaction between purified TRiC and HSF1 in vitro. Moreover, fluorescence anisotropy experiments using FITC coupled to HSF1A demonstrates that HSF1A–FITC binds to a purified Tcp1 subunit of TRiC with an affinity of approximately 600 nM. This is validated qualitatively via titration of purified Tcp1 into binding reactions containing 500 nM Biotin or HSF1A–Biotin [1]. Quantification bycounting the number of cell containing aggregates as a function of the total number of cells reveals that at HSF1A concentrations as low as 2 μM, a reduced number of aggregate–containing cells are observed. The fraction of cells containing aggregates continued to decrease in a dose–dependent manner such that pretreatment with 12 μM HSF1A resulta in ~20% of the cells exhibiting aggregates visible by fluorescence microscopy [2].In Vivo: HSF1A enhances HSF1 activity, stabilizes HSF1 expression and minimizes Doxorubicin (DOX)–induced cardiac damage. WKY rats are challenged with DOX (accumulated dose: 30 mg/kgw), and DOX combined with HSF1A (100 mg/kgw/day). Supplementation with HSF1A significantly elevates cardiac functions back to the levels of the control group. HSF1A has been shown to stimulate human HSF1 nuclear translocation, elevate protein chaperone expression and ameliorate protein misfolding and cell death in aneurodegenerative disease model. The echocardiographic results show that HSF1A also alleviates DOX–induced failures in cardiac function [3].PROTOCOL (Extracted from published papers and Only for reference)Kinase Assay:[1]Protein extracts are generated from mammalian, yeast and E. coli cultures using biotin–binding buffer (20 mM HEPES,5 mM MgCl 2, 1 mM EDTA, 100 mM KCl, 0.03% NP–40) supplemented with 1% Trition–X100 and protease inhibitors. Approximately 0.5mg of protein extract is incubated with 100 μM HSF1A–Biotin for 4 h at 4°C and HSF1A–Biotin associated proteins captured by with NeutrAvidin Agarose Resin. After washing in biotin binding buffer proteins are eluted using 50 μL biotin elution buffer (100 mM Tris,150 mM NaCl, 0.1 mM EDTA, 2 mM D–biotin), resolved on a 4–20% SDS–PAGE, and immunoblotted. For purified TRiC and Hsp70analyses, 5 nM protein is incubated in biotin–binding buffer+0.5% Triton X–100 with 100 μM biotin or 100 μM HSF1A–Biotin for 4 h at 4°C and captured with NeutrAvidin Resin. For NiNTA purified yeast Tcp1, different concentrations of Tcp1 0.5 μM, 1 mM, 2 mM, 3 mM and 4 mM in 25 mM Hepes pH 7.5, 150 mM NaCl are incubated with 0.5 μM Biotin or HSF1A–Biotin for 4 h at 4°C and captured with NeutrAvidin Resin [1].Cell Assay:[2]PC12 cells seeded into a 96–well plate (5×104 cells/well) are treated with increasing concentrations of HSF1A (2, 4, 8 andProduct Name:HSF1A Cat. No.:HY-103000CAS No.:1196723-93-9Molecular Formula:C21H19N3O2S2Molecular Weight:409.52Target:HSP Pathway:Cell Cycle/DNA Damage; Metabolic Enzyme/Protease Solubility:DMSO: ≥ 150 mg/mL12 μM) for 15 h, at which time httQ74–GFP expression is stimulated by incubation in the presence of 1 μg/mL Doxycycline for 5 d. Cell viability is assessed via the XTT viability assay[2].Animal Administration:[3]Rat[3]Ten–week–old Wistar Kyoto rats (WKY) are used. The rats are housed at a constant temperature (22°C) on a 12–h light/dark cycle with food and tap water. The animals are arranged into three groups: WKY rats (the control group), DOX rats and DOX rats treated with HSF1A. Each group contain five animals. The DOX group is injected with DOX (5 mg/kg) for 6 consecutive weeks intraperitoneal injection to achieve a cumulative dose of 30 mg/kg, which has been well documented to achieve cardiotoxicity. The small molecular HSF1 activator HSF1A (100 mg/kg/day) is injected intraperitoneally.References:[1]. Neef DW, et al. A direct regulatory interaction between chaperonin TRiC and stress–responsive transcription factor HSF1. Cell Rep. 2014 Nov 6;9(3):955–66.[2]. Neef DW, et al. Modulation of heat shock transcription factor 1 as a therapeutic target for small molecule intervention in neurodegenerative disease. PLoS Biol. 2010 Jan 19;8(1):e1000291.[3]. Huang CY, et al. Doxorubicin attenuates CHIP–guarded HSF1 nuclear translocation and protein stability to trigger IGF–IIR–dependent cardiomyocyte death. Cell Death Dis. 2016 Nov 3;7(11):e2455.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。
迈克沃伊和法默的中国体外诊断分销指南说明书
McEvoy and Farmer's Complete Guide to IVD Distribution in Chinahttps:///r/W875106C98AEN.htmlDate: May 2009Pages: 240Price: US$ 3,995.00 (Single User License)ID: W875106C98AENAbstractsWho is Who in Clinical Diagnostics in China, produced jointly with the firm McEvoy & Farmer, is the product of on-the-ground primary research on Chinese labs conducted in fall and winter 2008. Included in this report:Market Size Estimate by Major Category (Chemistry, Critical Care Chemistry, Urine Chemistry, Hematology, Flow Cytometry, Coagulation, Immunochemistry, Molecular Testing, Other IVD)Country Industry OverviewChinese Hospital StatisticsProfiles of 42 International Diagnostic Companies with operations in ChinaProfiles of 130 Domestic Diagnostic CompaniesProfiles of 24 Local Distributors, indicating the companies they distribute forThe Chinese market represents a significant opportunity for IVD diagnostic companies. But actionable information about this emerging market is often difficult to get. Only with an exhaustive, on-the-ground research team can a company truly understand the chinese market. Now, a resource is available that can make on-the-ground research available to all companies at a fraction of the cost.Published jointly with trusted Asian IVD market experts, this Kalorama report is acomplete survey of the IVD market in China today. Market size for major categories of the IVD market, products on the market, important Chinese market trends and intense company profiles are part of this exhaustive study.This country of 1.3 billion people is now America’s sixth largest export market, and China’s economy, while showing some effects of the world recession, has been less impacted than other nations and is showing growth, although not as rapid as in recent years. A number of recent events and trends in the Chinese healthcare environment are making China an increasingly attractive market opportunity for in vitro diagnostics companies. The increasing numbers of private laboratories and expanded reference laboratories are expanding the market for tests of all kinds.Although there are a number of challenges for diagnostic manufacturers to understand and overcome, the market for clinical diagnostics in China (both reagents and instruments) remains one of the most promising emerging markets in the world.ContentsCOUNTRY SUMMARYMarket TotalsWhat is New in ChinaThe Healthcare System in ChinaMedical InsuranceA Brief Guide to the BureaucracyPrivate and Reference LaboratoriesPrivate Medical PracticeProduct RegistrationReimbursementTendersReagent RentalQuality ControlVacuum Tube ConversionSecond hand InstrumentsImported and Domestic Sales INTERNATIONAL MANUFACTURER PROFILESAbbott LaboratoriesABXAcon Biotech/InvernessAdaltisAffymetrixAgilent TechnologiesApplied BiosystemsArkrayAudit DiagnosticsBeckman CoulterBDBioneer TradeBio Rad LaboratoriesbioMérieuxDiaSorinDiaSys Diagnostic SystemsEppendorfEuroimmunFujirebio/CanAg DiagnosticsHitachi High Technologies CorporationHospitexHumanInverness MedicalJei Daniel (JD) BiotechJokohMedicine Devices Company (MDC) Melet Schloesing LaboratoriesMP BiomedicalsOrtho ClinicalDiagnosticsPerkinElmerPromegaQiagenRadiometerRandoxR BiopharmRocheSiemens Healthcare Diagnostics StagoSysmex CorporationThermoFisherVirionSerionYD DiagnosticsDOMESTIC MANUFACTURER PROFILES3V BioengineeringAccuBio TechAddcare Bio TechAi De Diagnostic (IND)AmpllyAntai DiagnosticsAudicom Medical InstrumentAutobio DiagnosticsAVE Science & TechnologyB & E Scientific InstrumentBasoDiagnosticsBeijing Genomics Institute (BGI Healthcare)Biocell InstituteBiocreateBiocup Biotech CompanyBioer TechnologyBiosino BiotechnologyBiote CompanyBioway BiotechnologyBlue Cross Bio MedicalBowlinman Sunshine Science & TechnologyCaihong Analytical InstrumentCaltech GroupCapitalBio CorporationCaretium Medical InstrumentsChang Chun Brother BiotechChangdao BiotechnologyChemClin Bio Tech / China Diagnostics Medical Chemtron BiotechChina Medical/Yuande Bio Medical EngineeringCondor Teco Medical TechnologyCornley Hi TechDa An GeneDecipher BioscienceDirui IndustrialDL Medical BiotechDoubleQ LabDragon MedicalElikan Biological TechnologyFenghua BioengineeringFengHui Medical Science & Technology First Sun ElectronicFosun DiagnosticsGenetel PharmaceuticalsGenius ElectronicsGoldsite DiagnosticsHai Tai BiologicalHaoyuan BiotechnologyHeal Force/Nison InstrumentHealthDigitHongcheng (HC) BiopharmaceuticalHongshi Medical TechnologyHope Industry and TradeHua Sin ScienceHua Tong Medical InstrumentHuaguan Biochip CompanyHuatai Biotechnology IndustrialHuayang Analysis InstrumentHybriBioInTec ProductsJian Ye Medical EquipmentinSangTe Medicine InstrumentKanghua Biotech CompanyKehua Bio EngineeringKinghawk TechnologyLabnovation TechnologiesLandwind International Medical Science LaoLa ElectronicLeadman Biochemistry TechnologyLengguang TechnologyLingyi Medical ScienceLivzon GroupLongx TechnologiesMaker Science TechnologyMaysun TechnologyMaxcom ElectronicMD Pacific TechnologyMeiyilin Electronics InstrumentMerit Choice BioengineeringMindray Medical ElectronicsModern Gold BiotechnologyNanfen Medical Biochemical InstrumentNeusoft Medical SystemsNew MoonNewScen Coast Bio PharmaceuticalOption Science & Technology Development Company Perlong GroupPG Biotech/QiagenPrecil InstrumentProcan ElectronicsRayto Life and Analytical SciencesRich Science IndustryRongsheng Biotech.Runbio Biotech .Sanco InstrumentSan Jose Medical Products..Sciarray BiotechSciendox Bio-Technology CompanySenlong Biotech..Share SunShensuo Medical DiagnosticsShenzhen New Industries Biomedical Engineering (SNIBE) Shining Sun TechnologySinnowa Medical Science & TechnologySTAC Medical Science & TechnologySteellex Scientific InstrumentStrong Biotechnologies...Success Technology DevelopmentSun BiotechSunostik Biomedical TechnologySym-Bio Lifescience.Tecom ScienceTechcompTellgen LifeTiangen Biotech...TianHai Medical Equipment (THME)Tianlong Science and TechnologyTigsun Biotinge Science & TechnologyTZD Technological.Urit Medical ElectronicWanCheng Bio-elect.Wantai BiologicalWasson An-Ze Bio-tech Company..WearmaxWeirikang Biological TechnologyW.H.P.M. Bioresearch & Technology/Hemosure Wondfo BiotechXun-Da Medical InstrumentYasen IndustrialYaxin Sheng WuZhong Tai BiotechZJ Bio-TechDISTRIBUTOR PROFILESAdvanced Clinical Laboratory Science (ACLS)Ailex Technology.....AusBio LaboratoriesBio-Asia DiagnosticsBiochem GroupBio-Star Technology DevelopmentChindex InternationalChinMax Medical SystemsDiamond BiotechnologyDong Hu Instrument/East LakeFu Li Tai (FLT) MeditecGene CompanyGiantech Medical Science & TechnologyGolden-Grand Medical Hongtex Bio-techLangkaNewtime TradingRainbow-Mega Scientific InstrumentScience International/Science LaboratoriesSunlionSuns-GroupTruth EnterpriseUnited Science InternationalVastec Medical..Zhi Cheng Biotech.Appendix: China’s Hospitals by Province.I would like to orderProduct name:McEvoy and Farmer's Complete Guide to IVD Distribution in ChinaProduct link:https:///r/W875106C98AEN.htmlPrice:US$ 3,995.00 (Single User License / Electronic Delivery)If you want to order Corporate License or Hard Copy, please, contact our CustomerService:*************************PaymentTo pay by Credit Card (Visa, MasterCard, American Express, PayPal), please, clickbutton on product page https:///r/W875106C98AEN.htmlTo pay by Wire Transfer, please, fill in your contact details in the form below:First name:Last name:Email:Company:Address:City:Zip code:Country:Tel:Fax:Your message:**All fields are requiredCustumer signature _______________________________________Please, note that by ordering from you are agreeing to our Terms& Conditions at https:///docs/terms.htmlTo place an order via fax simply print this form, fill in the information belowand fax the completed form to +44 20 7900 3970。
抗体公司
赛信通（上海）生物试剂有限公司
上海市浦东南路1101号远东大厦514室，200120 info@cst www.cst 2158356288 公司总部: 美国
Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privatelyowned company with over 400 employees worldwide. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. Since its inception, CST has become the world leader in the production of the highest quality activationstate and total protein antibodies utilized to expand knowledge of cell signaling pathways. Our mission is to deliver the world's highest quality research tools that accelerate progress in biological research and personalized medicine. 总引用数为4670，来自于1966篇文章。最常引用的试剂包括： Akt, ERK2, ERK1, p38, Akt1。
AbD Serotec (BioRad)
GC测定盐酸普拉克索中三乙胺残留量
Trigonox 101-50D-PD 产品数据表说明书
Product Data SheetTrigonox 101-50D-PD 2,5-Dimethyl-2,5-di(tert-butylperoxy) hexaneTrigonox® 101-50D-PD is a 50% formulation on an inert carrier sytem in powder form.CAS number78-63-7EINECS/ELINCS No.201-128-1TSCA statuslisted on inventoryMolecular weight290.4Active oxygen contentperoxide11.02%Concentration5.40-5.62%SpecificationsAppearance White powderAssay49.0-51.0 %ApplicationsTrigonox® 101-50D-PD is a bifunctional peroxide which is used for the crosslinking of natural rubber and synthetic rubbers, as well as polyolefins. Rubber compounds containing Trigonox® 101-50D-PD have excellent scorch safety, and under certain conditions one step mixing is possible. Safe processing temperature: 135°C (rheometer ts2 > 20 min.). Typical crosslinking temperature: 175°C (rheometer t90 about 12 min.).Thermal stabilityOrganic peroxides are thermally unstable substances, which may undergo self-accelerating decomposition. The lowest temperature at which self-accelerating decomposition of a substance in the original packaging may occur is the Self-Accelerating Decomposition Temperature (SADT). The SADT is determined on the basis of the Heat Accumulation Storage Test.SADT80°CMethod The Heat Accumulation Storage Test is a recognized test method for thedetermination of the SADT of organic peroxides (see Recommendations on theTransport of Dangerous Goods, Manual of Tests and Criteria - United Nations, NewYork and Geneva).StorageDue to the relatively unstable nature of organic peroxides a loss of quality can be detected over a period of time. To minimize the loss of quality, Nouryon recommends a maximum storage temperature (Ts max. ) for each organic peroxide product.Ts Max.30°CTs Min.0°CNote When stored under these recommended storage conditions, Trigonox® 101-50D-PD will remain within the Nouryon specifications for a period of at least 6 monthsafter delivery.Packaging and transportThe standard packaging is a cardboard box for 20 kg peroxide formulationBoth packaging and transport meet the international regulations. For the availability of other packed quantities contact your Nouryon representative. Trigonox®101-50D-PD is classified as Organic peroxide type E; solid, Division 5. 2; UN 3108.Safety and handlingKeep containers tightly closed. Store and handle Trigonox® 101-50D-PD in a dry well-ventilated place away from sources of heat or ignition and direct sunlight. Never weigh out in the storage room. Avoid contact with reducing agents (e. g. amines), acids, alkalis and heavy metal compounds (e. g. accelerators, driers and metal soaps). Please refer to the Safety Data Sheet (SDS) for further information on the safe storage, use and handling of Trigonox® 101-50D-PD. This information should be thoroughly reviewed prior to acceptance of this product. The SDS is available at /sds-search.Major decomposition productsMethane, Ethane, Acetone, tert-Butanol, tert-AmylalcoholAll information concerning this product and/or suggestions for handling and use contained herein are offered in good faith and are believed to be reliable.Nouryon, however, makes no warranty as to accuracy and/or sufficiency of such information and/or suggestions, as to the product's merchantability or fitness for any particular purpose, or that any suggested use will not infringe any patent. Nouryon does not accept any liability whatsoever arising out of the use of or reliance on this information, or out of the use or the performance of the product. Nothing contained herein shall be construed as granting or extending any license under any patent. Customer must determine for himself, by preliminary tests or otherwise, the suitability of this product for his purposes.The information contained herein supersedes all previously issued information on the subject matter covered. The customer may forward, distribute, and/or photocopy this document only if unaltered and complete, including all of its headers and footers, and should refrain from any unauthorized use. Don’t copythis document to a website.Trigonox® is a registered trademark of Nouryon Functional Chemicals B.V. or affiliates in one or more territories.Contact UsPolymer Specialties Americas************************Polymer Specialties Europe, Middle East, India and Africa*************************Polymer Specialties Asia Pacific************************2022-6-30© 2022Polymer crosslinking Trigonox 101-50D-PD。
冰冻切片胶原纤维(MASSON)染色试剂盒产品说明书
GENMED SCIENTIFICS INC. U.S.A GMS80013.2 v.A GENMED冰冻切片胶原纤维（MASSON）染色试剂盒产品说明书（中文版）主要用途GENMED冰冻切片胶原纤维（MASSON）染色试剂是一种旨在使用胞核－胞浆－胶原蛋白三色染料（trichrome），分析和区分存档中的冰冻组织切片中的胶原纤维（collagen fiber）的权威而经典的技术方法。
该技术由大师级科学家精心改良传统方法、成功实验证明的。
主要适用于胶原蛋白和平滑肌的鉴别分析。
广泛用于结缔组织、肌肉组织和胶原蛋白的研究等。
产品严格无菌，即到即用，操作简捷，性能稳定，显色清晰。
技术背景基于摩罗利（mallory）首次应用三色系统的方法，马森（masson）进行了改良，在三色复合染料体系中，使用苯胺篮（aniline blue）替代绿篮，作为胶原蛋白的染色。
三色复合染料选择性地染色肌肉、胶原纤维、纤维蛋白（fibrin）和红细胞。
肝肾疾病，例如纤维化（cirrhsis）等，其胶原蛋白增多。
产品内容GENMED清理液（Reagent A）XX毫升GENMED包氏液（Reagent B）XX毫升GENMED韦格液（Reagent C）XX毫升GENMED比氏液（Reagent D）XX毫升GENMED酸性液（Reagent E）XX毫升GENMED染色液（Reagent F）XX毫升GENMED修正液（Reagent G）XX毫升产品说明书1份保存方式保存在4℃冰箱里，避免光照；试剂具有腐蚀性，注意安全；有效保证6月用户自备小型玻璃染色缸：用于组织或切片处理的容器培养箱：用于样品反应孵育微波炉：用于样品反应孵育处理载玻片和盖玻片：用于切片后铺片中性树脂：用于切片封片光学显微镜：用于切片染色后观察分析实验步骤操作一：标准染色1．准备好XX微米厚的冰冻切片2．小心加上XX微升GENMED清理液（Reagent A）在切片上，铺满整个切片样品表面3．室温下孵育XX分钟4．小心移去切片上的GENMED清理液（Reagent A）5．小心加入XX微升GENMED包氏液（Reagent B）在切片上，铺满整个切片样品表面6．放进60℃恒温培养箱孵育XX分钟（注意：避免干枯；如果强化效果，可以室温孵育16小时） 7．小心移去GENMED包氏液（Reagent B）8．室温下，小心将切片置入XX毫升GENMED清理液（Reagent A）中孵育2分钟9．小心移去切片上的GENMED清理液（Reagent A）10．小心加入XX微升GENMED韦格液（Reagent C）在切片上，铺满整个切片样品表面11．室温下孵育XX分钟12．小心移去GENMED韦格液（Reagent C）13．室温下，小心将切片置入XX毫升GENMED清理液（Reagent A）中孵育XX分钟14．小心移去切片上的GENMED清理液（Reagent A）15．小心加入XX微升GENMED比氏液（Reagent D）在切片上，铺满整个切片样品表面16．室温下孵育XX分钟（注意：可以延长至15分钟，增强染色效果）17．小心移去GENMED比氏液（Reagent D）18．小心加入XX微升GENMED清理液（Reagent A）在切片上，铺满整个切片样品表面19．小心移去切片上的GENMED清理液（Reagent A）20．重复实验步骤18至19二次21．小心加入XX微升GENMED酸性液（Reagent E）在切片上，铺满整个切片样品表面（注意：可以孵育15分钟，增强染色效果）22．小心移去切片上的GENMED酸性液（Reagent E）23．小心加上XX微升 GENMED染色液（Reagent F）在切片上，铺满整个切片样品表面24．室温下孵育XX分钟，避免光照（注意：可以延长至10分钟，增强染色效果）25．小心移去切片上的GENMED染色液（Reagent F）26．小心加上XX微升 GENMED修正液（Reagent G）在切片上，铺满整个切片样品表面27．室温下孵育XX分钟28．小心移去切片上的GENMED修正液（Reagent G）29．室温下，小心将切片置入XX毫升GENMED清理液（Reagent A）中孵育XX分钟30．小心移去切片上的GENMED清理液（Reagent A）31．放上盖玻片或封片（中性树脂）32．即刻在一般光学显微镜下观察：细胞核――呈现黑色细胞质――呈现红色肌纤维――呈现红色红细胞――呈现红色胶原纤维――呈现蓝色操作二：热处理染色1．准备好XX微米厚的冰冻切片2．小心加上XX微升GENMED清理液（Reagent A）在切片上，铺满整个切片样品表面 3．室温下孵育XX分钟4．小心移去切片上的GENMED清理液（Reagent A）5．准备1个XX毫升烧杯或小型染色缸6．加入XX毫升GENMED包氏液（Reagent B）7．小心放进上述脱腊处理的切片8．放进微波炉（600瓦）加热XX分钟9．室温下静置15分钟10．取出切片，小心移去切片上的GENMED包氏液（Reagent B）11．室温下，小心将切片置入XX毫升GENMED清理液（Reagent A）中孵育5分钟 12．小心移去切片上的GENMED清理液（Reagent A）13．小心加入XX微升GENMED韦格液（Reagent C）在切片上，铺满整个切片样品表面 14．室温下孵育XX分钟15．小心移去GENMED韦格液（Reagent C）16．室温下，小心将切片置入XX毫升GENMED清理液（Reagent A）中孵育5分钟 17．小心移去切片上的GENMED清理液（Reagent A）18．小心加入XX微升GENMED比氏液（Reagent D）在切片上，铺满整个切片样品表面 19．室温下孵育XX分钟（注意：可以延长至15分钟，增强染色效果）20．小心移去GENMED比氏液（Reagent D）21．小心加入XX微升GENMED清理液（Reagent A）在切片上，铺满整个切片样品表面 22．小心移去切片上的GENMED清理液（Reagent A）23．重复实验步骤21至22二次24．小心加入XX微升GENMED酸性液（Reagent E）在切片上，铺满整个切片样品表面 25．室温下孵育XX分钟（注意：可以延长至15分钟，增强染色效果）26．小心移去切片上的GENMED酸性液（Reagent E）27．小心加上XX微升 GENMED染色液（Reagent F）在切片上，铺满整个切片样品表面 28．室温下孵育XX分钟，避免光照（注意：可以延长至10分钟，增强染色效果） 29．小心移去切片上的GENMED染色液（Reagent F）30．小心加上XX微升 GENMED修正液（Reagent G）在切片上，铺满整个切片样品表面 31．室温下孵育XX分钟32．小心移去切片上的GENMED修正液（Reagent G）33．室温下，小心将切片置入XX毫升GENMED清理液（Reagent A）中孵育2分钟 34．小心移去切片上的GENMED清理液（Reagent A）35．放上盖玻片或封片（中性树脂）36．即刻在一般光学显微镜下观察：细胞核――呈现黑色细胞质――呈现红色肌纤维――呈现红色红细胞――呈现红色胶原纤维――呈现蓝色注意事项1．本产品为50次操作2．操作时，须戴手套3．试剂具有腐蚀性，注意操作安全4．建议使用玻璃染色缸5．每次更换试剂溶液时，保持切片面基本晾干6．试剂溶液在切片表面时，避免有气泡存在，同时确保铺满切片表面7．整个操作，在避光状态下进行8．染色完成后，即刻进行光学显微镜观察9．样品染色后保存，避免光照10．本公司提供系列特定组织染色试剂产品质量标准1．本产品经鉴定性能稳定2．本产品经鉴定显色清晰使用承诺杰美基因秉着“信誉至上、客户满意、质量承诺”的宗旨为我们的用户提供优质产品和服务。
Luc-Pair
Luc-Pair™Duo-Luciferase HS Assay Kit-高灵敏性双荧光素酶检测试剂盒Cat.No.LF004(100reactions)Cat.No.LF005(300reactions)Cat.No.LF006(1000reactions)使用说明书GeneCopoeia,Inc.广州易锦生物技术有限公司9620Medical Center Drive,#101地址：广州科学城揽月路3号F区F801（510663）Rockville,MD20850电话：4006-020-200、************、************ USA网站：301-762-0888866-360-9531***********************©2016GeneCopoeia,Inc.使用说明书Luc-Pair™Duo-Luciferase HS Assay KitI.产品概述II.产品信息及储存条件III.细胞裂解IV.FLuc和RLuc工作液的配制V.荧光素酶检测流程VI.有限使用许可及质保声明I.产品概述对报告基因表达的转录调控的研究常被应用于生物学研究和药物发现。
荧光素酶在基因表达研究中应用最为广泛，其主要包含以下几个优点：1)在广泛动态范围内具有高灵敏度2)在哺乳动物细胞内无荧光素酶、背景极低3)实验重复性好4)成本低5)操作简单萤火虫和海肾荧光素酶都具有快捷、简便、灵敏度高的检测特点，被公认为是理想的报告基因，因为它们具有完全不同的进化起源、酶学结构和底物要求。
萤光素酶报告基因的测定需要用光度计或多功能微孔板检测仪，且发光强度与荧光素酶的数量成正比。
萤火虫荧光素酶（Photinus pyralis）已被证实是检测启动子活性和监测基因转录后调控状态的理想的报告基因。
它是在细胞质中作用的酶，分子量为61kDa并催化下列反应：海肾（Renilla reniformis）荧光素酶是一个36kDa单亚基蛋白质，酶活性不需要翻译后修饰，因此它可以作为一个实时转录报告基因，催化下面的生物发光反应：此体系可以在目的基因的附近监控顺式作用元件的转录激活。
Santa Cruz Biotechnology产品说明书
In complete Ad j u v ant (10x10ml): s c -24019CH EMICAL IDENTIFICATIO NSynonyms: Celex, Celloidin, Cellulose Tetranitrate, Kodak LR 115, Paralodion, Pyralin, Proxylin, and Xyloidin.Description: Complete Freund’s Adjuvant (CFA) is a water-in-oil emulsion t hat contains Mycobacterium tuberculosis cell wall components. A djuvant activity results from sustained release of immunogen from the oily deposit and stimulation of a local immune response. CFA is used for t he initial inject ions. T o minimize side-effects, Incomplete Freund’s Adjuvant, lacking Mycobacterium tuberculosis cell wall components, is used for subsequent boosts.References:Tubercle 58,4: 221-224 (1977).CO MPOSITION/INFO RMATIO N O N INGRED IENTS CAS #: noneStorage: Store container in a cool, well-ventilated area.HAZARD S IDENTIFICATIONPhysical State and Appearance: Liquid. (liquid until emulsified with antigen when it takes on a milky color.)Medical Conditions Aggravated by O verexposure: Repeated or prolonged exposure is not known to aggravate medical condition.FIRST-AID MEASURESIngestion: Do not induce vomiting unless directed to do so by medical personnel. N ever give anythin g by mou th to an unconscious person. If large quantities of t his material are swallowed, call a physician immediately. Loosen tight clothing such as a collar, tie, belt or waistband.Eye Contact: Check for and remove any contact lenses. In case of contact, immediately flush eyes with plenty of water for at least 15minutes. Get medical attention.Skin Contact: In case of contact, immediately flush skin with plenty of water. Remove contaminated clothing and shoes. Wash clothing before reuse. Thoroughly clean shoes before reuse. Get medical attention.Inhalation: If inhaled, remove to fresh air. If not breathing, giv e artificial respiration. If breathing is difficult, give oxygen. Get medical attention.ACCID ENT AL RELEASE MEASURESWear protective equipment. Absorb on sand or vermiculite and place in closed containers for disposal. Ventilate area and wash spill site after material pickup is complete.FIRE EXTINGUISHING MEASURESExtinguishing media: carbon dioxide, dry chemical powder or appropriate foam.Special firefighting procedures: wear self-contained breathing apparatus and protective clothing to prevent contact with skin and eyes.H ANDLING AND STO RAGEHandling: Keep container tightly closed. Keep container in a cool,well-ventilated area.Storage: Avoid breathing vapors or spray mists.EXPOSURE CO NTROLS/PERSONAL PROTECTIO N Wear appropriate NIOSH /MSH A -approved respirator, chemical-resistan t gloves, safet y goggles, ot her protect ive clothin g.Mechanical exhaust required.PHYSICAL AND CHEMICAL PROPERTIES Appearance and odor: amber oil STABILITY AND REACTIVITY Stability: stable.Protect from light.H azardous combustion or decomposition products: nature of decomposition products not known; hazardous polymerization will not occur.TO XICOLOGICAL IN FORMATIONAcute effects: may be harmful by inhalation, ingestion, or skin absorption. the toxicological properties have not been thoroughly investigated.ECO LO GICAL INFORMATION Data not yet available.DISPOSAL CONSIDERATIONSDissolve or mix the material with a combustible solvent and burn in a chemical incinerator equipped with an afterburner and scrubber. O bserve all federal, state and local environmental regulations.TRANSPORT INFORMATIONContact Santa Cruz Biotechnology for transportation information.Material Safety Data SheetREGULATORY INFORMATIONU.S. Federal RegulationsTSCA: No products were found.Clean Water Act (CWA) 307: No products were found.Clean Water Act (CWA) 311: No products were found.Clean air act (CAA) 112 accidental release prevention: N o products were found.Clean air act (CAA) 112 regulated flammable substances: N o products were found.Clean air act (CAA) 112 regulated toxic substances: No products were found.SARA 302/304/311/312 extremely hazardous substances: N o products were found.SARA 302/304 emergency planning and notification: No products were found.SARA 302/304/311/312 hazardous chemicals: No products were found.SARA 311/312 MSDS distribution - chemical inventory - hazard identification: No products were found.SARA313 toxic chemical notification and release reporting: No products were found.OTHER INFORMATIONThe above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. Santa Cruz Biotechnology shall n ot be held liable for any damage resulting from handling or from contact with the product.。
IFCC Aspartate Aminotransferase 检测手册说明书
ASTAspartate Aminotransferase IFCCMANUAL RX MONZAINTENDED USEFor the quantitative in vitro determination of AspartateAminotransferase (AST) in serum and plasma. This product is suitable for manual use and on the Rx Monza analyser.Cat. No. AS 1202 R1a. Buffer/Substrate 1 x 70 ml 20 x 2 ml R1b. Enzyme/Coenzyme/ 20 x 2 ml α-oxoglutarate GTIN: 05055273200416AS 1204 R1a. Buffer/Substrate 1 x 105 ml 10 x 10 ml R1b. Enzyme/Coenzyme/ 10 x 10 ml α-oxoglutarate GTIN: 05055273200423AS 1267 R1a. Buffer/Substrate 1 x 105 ml 5 x 20 ml R1b. Enzyme/Coenzyme/ 5 x 20 ml α-oxoglutarate GTIN: 05055273200430AS 2359 R1a. Buffer/Substrate 5 x 100 ml 5 x 100 ml R1b. Enzyme/Coenzyme/ 5 x 100 ml α-oxoglutarate GTIN: 05055273200454UV METHODThis is an optimised standard method according to the concentrations recommended by the IFCC.CLINICAL SIGNIFICANCE (1,2,3,4)The aminotransferases are a group of enzymes that catalyse the inter conversions of amino acids and α-oxoacids by transfer of amino groups. AST (aspartate aminotransferase or glutamate oxaloacetatetransaminase) has been found in the cytoplasm and the mitochondria of cells that have been studied. In cases of mild tissue damage, e.g. liver, the predominant form of serum AST is that from the cytoplasm, with a smaller amount coming from the mitochondria. Severe tissue damage will result in more mitochondrial enzyme being released. Elevated levels of AST can signal myocardial infarction, hepatic disease, muscular dystrophy and organ damage.Although heart muscle is found to have the most activity of the enzyme, significant activity has also been seen in the brain, liver, gastric mucosa, adipose tissue and kidneys of humans.The IFCC has now recommended (1980) standardised procedures for AST determinations including:-1. optimization of substrate concentrations.2. Employment of Tris buffers (instead of phosphate, which has beenshown to inhibit recombination of the apoenzyme with pyridoxal phosphate).3. Pre-incubation of combined buffer and serum to allow sidereactions with NADH to occur. 4. Substrate start (α-oxoglutarate)5. Optional pyridoxal phosphate activation.This is an optimised standard method according to the recommendations of the IFCC.PRINCIPLEα-oxoglutarate reacts with L-aspartate in the presence of AST to form L-glutamate plus oxaloacetate. The indicator reaction utilises the oxaloacetate for a kinetic determination of NADH consumption. AST -oxoglutarate + L-aspartate L-glutamate + oxaloacetate MDH oxaloacetate + NADH + H + L-malate + NAD +SPECIMEN COLLECTION AND PREPARATION (5) Serum:- Use serum free from haemolysis.Plasma:- EDTA or heparin can be used as the anticoagulant.Plasma should be separated from cells within one hour after collection.Specimens should be refrigerated if not used immediately:-Specimens stored longer than 3 days should be frozen at -20︒C.REAGENT COMPOSITIONContents Concentrations in the TestR1a. Buffer/Substrate Tris buffer 80 mmol/l, pH 7.5 L-aspartate 240 mmol/l R1b. Enzyme/Coenzyme/α-oxoglutarate α-oxoglutarate 12 mmol/l MDH ≥420 U/l LD ≥600 U/l NADH 0.18 mmol/lSAFETY PRECAUTIONS AND WARNINGS For in vitro diagnostic use only. Do not pipette by mouth.Exercise the normal precautions required for handling laboratory reagents.Solution R1a contains Sodium Azide. Avoid ingestion or contact with skin or mucous membranes. In case of skin contact, flush affected area with copious amounts of water. In case of contact with eyes or if ingested, seek immediate medical attention.Sodium Azide reacts with lead and copper plumbing, to form potentially explosive azides. When disposing of such reagents flush with large volumes of water to prevent azide build up. Exposed metal surfaces should be cleaned with 10% sodium hydroxide.Health and Safety data sheets available on request.The reagents must be used only for the purpose intended by suitably qualified laboratory personnel, under appropriate laboratory conditions.STABILITY AND PREPARATION OF REAGENTS R1a. Buffer/SubstrateContents ready for use. Stable up to the expiry date when stored at +2 to +8︒C.R1b. Enzyme/Coenzyme/α-oxoglutarate Reconstitute one vial of Enzyme/Coenzyme/α-oxoglutarate R1b with the appropriate volume of Buffer/Substrate R1a: 2 ml for the 20 x 2 ml kit (AS 1202) 10 ml for the 10 x 10 ml kit (AS 1204) 20 ml for the 5 x 20 ml kit (AS 1267) Stable for 14 days at +2 to +8︒C or 24 hours at +15 to +25︒C. Cat. AS 2359 5 x 100 mlReconstitute one vial of Enzyme/Coenzyme/α-oxoglutarate R1b with a portion of Buffer/Substrate R1a and then transfer the entire contents to bottle R1a rinsing bottle R1b several times. Stable for 14 days at +2 to +8︒C or 24 hours at +15 to +25︒C.MATERIALS PROVIDED Buffer/SubstrateEnzyme/Coenzyme/ -oxoglutarateMATERIALS REQUIRED BUT NOT PROVIDEDRandox Assayed Multisera Level 2 (Cat. No. HN 1530) and Level 3 (Cat. No. HE 1532)Randox Calibration Serum Level 3 (Cat. No. CAL 2351) RX series Saline (Cat. No. SA 3854)PROCEDUREAspirate fresh ddH 2O and perform a new Gain Calibration in flow cell mode. Select AST in the Run Test screen and carry out a water blank as instructed.Pipette into a test tube:Sample 0.05 ml Reagent 0.5 mlMix and aspirate into the Rx Monza.CALIBRATION FOR RX MONZAThe use of Saline and Randox Calibration Serum Level 3 isrecommended for calibration. Calibration is recommended with change of reagent lot or as indicated by quality control procedures.FOR MANUAL USEWavelength: 340 nm (Hg 334 nm or Hg 365 nm) Cuvette: 1 cm light path Temperature: 25/30/37︒C Measurement: against airPipette into cuvette: Macro MicroSample 0.2 ml 0.1 ml Enzyme/Coenzyme/ α-oxoglutarate R1 2.0 ml 1.0 mlMix, read initial absorbance after 1 minute. Read again after 1, 2 and 3 minutes. Note: If the absorbance change per minute is between 0.11 and 0.16 at 340/Hg 334 nm 0.06 and 0.08 at Hg 365 nmuse only the values for the first 2 minutes for the calculation.MANUAL CALCULATIONTo calculate the AST activity, use the following formulae:U/l = 1746 x A 340 nm/min U/l = 1780 x A Hg 334 nm/min U/l = 3235 x A Hg 365 nm/minSTANDARDISATIONRandox Calibration Serum Level 3 is traceable to AST reference material JSCC TS01.QUALITY CONTROLRandox Assayed Multisera, Level 2 and Level 3 are recommended for daily quality control. Two levels of controls should be assayed at least once a day. Values obtained should fall within a specified range. If these values fall outside the range and repetition excludes error the following steps should be taken:1. Check instrument settings and light source.2. Check cleanliness of all equipment in use.3. Check water. Contaminants, i.e. bacterial growth, maycontribute to inaccurate results. 4. Check reaction temperature.5. Check expiry date of kit and contents.6. Contact Randox Laboratories Customer Technical Services, Northern Ireland +44 (0) 28 9445 1070.SPECIFICITY/INTERFERENCE (6,7)Gross haemolysis will produce falsely elevated test results. The effects of various drugs on AST activity should be taken intoconsideration in the case of patients receiving large doses of drugs.The analytes below were tested up to the following levels and were found not to interfere: Haemoglobin 250 mg/dl Free Bilirubin 25 mg/dl Conjugate Bilirubin 25 mg/dl Triglycerides 1000 mg/dlIntralipid ® 200 mg/dlA list of substances and conditions known to effect AST activity in vivo is given by both Young et al and Friedman et al. Norepresentation is made by Randox Laboratories Ltd regarding the completeness of these lists and the accuracy of the information contained therein.NORMAL VALUES IN SERUM (8,9) +25︒C +30︒C +37︒C Men up to 18 U/l up to 25 U/l up to 37 U/l Women up to 15 U/l up to 21 U/l up to 31 U/lIt is recommended that each laboratory establish its own reference range to reflect the age, sex, diet and geographical location of the population.SPECIFIC PERFORMANCE CHARACTERISTICS The following performance data were obtained using an Rx Monza analyser running at +37o C.LINEARITYThis method is linear up to 562 U/l. If the sample concentration exceeds this value, dilute the sample 1+9 with 0.9% NaCl solution and re-assay. Multiply the result by 10.SENSITIVITYThe minimum detectable concentration of AST with an acceptable level of precision was determined as 9.3 U/l.PRECISIONIntra AssayLevel 2 Level 3Mean (U/l) 35.6 153SD 1.66 1.47CV(%) 4.65 0.96n 20 20Inter AssayLevel 2 Level 3Mean (U/l) 35.6 153SD 1.77 7.10CV(%) 4.96 4.63n 20 20CORRELATIONThis method (Y) was compared with another commerciallyavailable method (X) and the following linear regression equationobtained:Y = 1.07X + 4.9and a correlation coefficient of r = 0.997543 patient samples were analysed spanning the range 28 to 559U/l.REFERENCES1. Wroblewski F, La Due J.S: Ann Intern Med. 1956; 45: 801.2. Wroblewski F, La Due J.S: Proc Soc Exp Biol Med 1956;91: 569.3. Bergmeyer HU, Bowers GN Jr, et al: Clin Chem 1977; 23:887.4. Bergmeyer HU, Bowers GN Jr, et al: J.Clin Chem ClinBiochem 1980; 18: 521-534.5. Tietz N W: Fundamentals of Clinical Chemistry ed 3.Philadelphia, WB Saunders Co. 1987, pg 372.6. Young D S, et al: Clin Chem 1975, 21; No5.7. Friedman RB, et al: Clin Chem 1980, 26; No4.8. Wallnofer H, Schmidt.E, Schmidt FW, eds: Synopsis derLeberkrankheiten Stuttgart, Georg Thieme Verlag, 1974.9. Thefeld W, et al: Dtsch Med Wschr 1974; 99: 343.Revised 26 Apr 16 biRev. 003THIS PAGE IS INTENTIONALLY BLANK。
HOE_32020_SDS_MedChemExpress
Inhibitors, Agonists, Screening LibrariesSafety Data Sheet Revision Date:May-24-2017Print Date:May-24-20171. PRODUCT AND COMPANY IDENTIFICATION1.1 Product identifierProduct name :HOE 32020Catalog No. :HY-15629CAS No. :23554-99-61.2 Relevant identified uses of the substance or mixture and uses advised againstIdentified uses :Laboratory chemicals, manufacture of substances.1.3 Details of the supplier of the safety data sheetCompany:MedChemExpress USATel:609-228-6898Fax:609-228-5909E-mail:sales@1.4 Emergency telephone numberEmergency Phone #:609-228-68982. HAZARDS IDENTIFICATION2.1 Classification of the substance or mixtureNot a hazardous substance or mixture.2.2 GHS Label elements, including precautionary statementsNot a hazardous substance or mixture.2.3 Other hazardsNone.3. COMPOSITION/INFORMATION ON INGREDIENTS3.1 SubstancesSynonyms:HOE32020; HOE⁻32020Formula:C25H23ClN6Molecular Weight:442.94CAS No. :23554-99-64. FIRST AID MEASURES4.1 Description of first aid measuresEye contactRemove any contact lenses, locate eye-wash station, and flush eyes immediately with large amounts of water. Separate eyelids with fingers to ensure adequate flushing. Promptly call a physician.Skin contactRinse skin thoroughly with large amounts of water. Remove contaminated clothing and shoes and call a physician.InhalationImmediately relocate self or casualty to fresh air. If breathing is difficult, give cardiopulmonary resuscitation (CPR). Avoid mouth-to-mouth resuscitation.IngestionWash out mouth with water; Do NOT induce vomiting; call a physician.4.2 Most important symptoms and effects, both acute and delayedThe most important known symptoms and effects are described in the labelling (see section 2.2).4.3 Indication of any immediate medical attention and special treatment neededTreat symptomatically.5. FIRE FIGHTING MEASURES5.1 Extinguishing mediaSuitable extinguishing mediaUse water spray, dry chemical, foam, and carbon dioxide fire extinguisher.5.2 Special hazards arising from the substance or mixtureDuring combustion, may emit irritant fumes.5.3 Advice for firefightersWear self-contained breathing apparatus and protective clothing.6. ACCIDENTAL RELEASE MEASURES6.1 Personal precautions, protective equipment and emergency proceduresUse full personal protective equipment. Avoid breathing vapors, mist, dust or gas. Ensure adequate ventilation. Evacuate personnel to safe areas.Refer to protective measures listed in sections 8.6.2 Environmental precautionsTry to prevent further leakage or spillage. Keep the product away from drains or water courses.6.3 Methods and materials for containment and cleaning upAbsorb solutions with finely-powdered liquid-binding material (diatomite, universal binders); Decontaminate surfaces and equipment by scrubbing with alcohol; Dispose of contaminated material according to Section 13.7. HANDLING AND STORAGE7.1 Precautions for safe handlingAvoid inhalation, contact with eyes and skin. Avoid dust and aerosol formation. Use only in areas with appropriate exhaust ventilation.7.2 Conditions for safe storage, including any incompatibilitiesKeep container tightly sealed in cool, well-ventilated area. Keep away from direct sunlight and sources of ignition.Recommended storage temperature:Powder-20°C 3 years4°C 2 yearsIn solvent-80°C 6 months-20°C 1 monthShipping at room temperature if less than 2 weeks.7.3 Specific end use(s)No data available.8. EXPOSURE CONTROLS/PERSONAL PROTECTION8.1 Control parametersComponents with workplace control parametersThis product contains no substances with occupational exposure limit values.8.2 Exposure controlsEngineering controlsEnsure adequate ventilation. Provide accessible safety shower and eye wash station.Personal protective equipmentEye protection Safety goggles with side-shields.Hand protection Protective gloves.Skin and body protection Impervious clothing.Respiratory protection Suitable respirator.Environmental exposure controls Keep the product away from drains, water courses or the soil. Cleanspillages in a safe way as soon as possible.9. PHYSICAL AND CHEMICAL PROPERTIES9.1 Information on basic physical and chemical propertiesAppearance Light green to green (Solid)Odor No data availableOdor threshold No data availablepH No data availableMelting/freezing point No data availableBoiling point/range No data availableFlash point No data availableEvaporation rate No data availableFlammability (solid, gas)No data availableUpper/lower flammability or explosive limits No data availableVapor pressure No data availableVapor density No data availableRelative density No data availableWater Solubility No data availablePartition coefficient No data availableAuto-ignition temperature No data availableDecomposition temperature No data availableViscosity No data availableExplosive properties No data availableOxidizing properties No data available9.2 Other safety informationNo data available.10. STABILITY AND REACTIVITY10.1 ReactivityNo data available.10.2 Chemical stabilityStable under recommended storage conditions.10.3 Possibility of hazardous reactionsNo data available.10.4 Conditions to avoidNo data available.10.5 Incompatible materialsStrong acids/alkalis, strong oxidising/reducing agents.10.6 Hazardous decomposition productsUnder fire conditions, may decompose and emit toxic fumes.Other decomposition products - no data available.11.TOXICOLOGICAL INFORMATION11.1 Information on toxicological effectsAcute toxicityClassified based on available data. For more details, see section 2Skin corrosion/irritationClassified based on available data. For more details, see section 2Serious eye damage/irritationClassified based on available data. For more details, see section 2Respiratory or skin sensitizationClassified based on available data. For more details, see section 2Germ cell mutagenicityClassified based on available data. For more details, see section 2CarcinogenicityIARC: No component of this product present at a level equal to or greater than 0.1% is identified as probable, possible or confirmed human carcinogen by IARC.ACGIH: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by ACGIH.NTP: No component of this product present at a level equal to or greater than 0.1% is identified as a anticipated or confirmed carcinogen by NTP.OSHA: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by OSHA.Reproductive toxicityClassified based on available data. For more details, see section 2Specific target organ toxicity - single exposureClassified based on available data. For more details, see section 2Specific target organ toxicity - repeated exposureClassified based on available data. For more details, see section 2Aspiration hazardClassified based on available data. For more details, see section 212. ECOLOGICAL INFORMATION12.1 ToxicityNo data available.12.2 Persistence and degradabilityNo data available.12.3 Bioaccumlative potentialNo data available.12.4 Mobility in soilNo data available.12.5 Results of PBT and vPvB assessmentPBT/vPvB assessment unavailable as chemical safety assessment not required or not conducted.12.6 Other adverse effectsNo data available.13. DISPOSAL CONSIDERATIONS13.1 Waste treatment methodsProductDispose substance in accordance with prevailing country, federal, state and local regulations.Contaminated packagingConduct recycling or disposal in accordance with prevailing country, federal, state and local regulations.14. TRANSPORT INFORMATIONDOT (US)This substance is considered to be non-hazardous for transport.IMDGThis substance is considered to be non-hazardous for transport.IATAThis substance is considered to be non-hazardous for transport.15. REGULATORY INFORMATIONSARA 302 Components:No chemicals in this material are subject to the reporting requirements of SARA Title III, Section 302.SARA 313 Components:This material does not contain any chemical components with known CAS numbers that exceed the threshold (De Minimis) reporting levels established by SARA Title III, Section 313.SARA 311/312 Hazards:No SARA Hazards.Massachusetts Right To Know Components:No components are subject to the Massachusetts Right to Know Act.Pennsylvania Right To Know Components:No components are subject to the Pennsylvania Right to Know Act.New Jersey Right To Know Components:No components are subject to the New Jersey Right to Know Act.California Prop. 65 Components:This product does not contain any chemicals known to State of California to cause cancer, birth defects, or anyother reproductive harm.16. OTHER INFORMATIONCopyright 2017 MedChemExpress. The above information is correct to the best of our present knowledge but does not purport to be all inclusive and should be used only as a guide. The product is for research use only and for experienced personnel. It must only be handled by suitably qualified experienced scientists in appropriately equipped and authorized facilities. The burden of safe use of this material rests entirely with the user. MedChemExpress disclaims all liability for any damage resulting from handling or from contact with this product.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。
TEDA-L33E商品说明书
SAFETY DATA SHEET: TEDA – L33E – US-GHS VersionTEDA - L33E1. IDENTIFICATION OF THE SUBSTANCE OR MIXTURE AND OF THE SUPPLIERPRODUCT IDENTIFIER: TEDA – L33EMANUFACTURER / IMPORTER:TOSOH SPECIALTY CHEMICALS USA, Inc. ADDRESS: 1720 Windward Concourse, Suite 125Alpharetta, Georgia 30005 PHONE: 1-770-442-9501EMERGENCY PHONE :CHEMTREC 1-800-424-9300 OR 1-703-527-3887RECOMMENDED USE:General industrial products2. HAZARDS IDENTIFICATIONGHS CLASSIFICATIONAcute toxicityOral: Category 4 Skin corrosion/irritation Category 2 Serious eye damage/eye irritation Category 2A Specific target organ toxicity – single exposure Category 3 Specific target organ toxicity – repeat exposure Category 2HAZARD SYMBOL:SIGNAL WORD : WARNINGHAZARD STATEMENTS :Harmful if swallowed. Causes skin irritation.Causes serious eye irritation.May cause drowsiness or dizziness.May cause damage to kidneys through prolonged or repeated exposure.PREVENTION :Wash thoroughly after handling.Do not eat, drink or smoke when using thisproduct.Wear protective gloves/eye protection/faceprotection.Avoid breathingdust/fume/gas/mist/vapors/spray.Use only outdoors or in a well-ventilated area.distributed by:Request Quote or SamplesSAFETY DATA SHEET: TEDA – L33E – US-GHS Version2. HAZARDS IDENTIFICATION (continued)RESPONSE :If in eyes: Rinse cautiously with water for several minutes.Remove contact lenses, if present and easy to do.Continue rinsing.If eye irritation persists: Get medical advice/attention.If on skin (or hair): Wash with plenty of water. If skin irritation occurs: Get medical advice/attention.Take off contaminated clothing and wash it before reuse.If inhaled: Remove person to fresh air and keep comfortable for breathing.Call a poison control center/doctor if you feel unwell.If swallowed: Rinse mouth.Call a poison control center/doctor if you feel unwell.STORAGE: Store in a well-ventilated place. Keep container tightly closed.Store locked up.DISPOSAL :Dispose of contents/container in accordance with Federal and state regulations.3. COMPOSITION/INFORMATION ON INGREDIENTSOSHAChemical Name CAS # Hazardous(Y/N) Concentration (%) Triethylenediamine 280-57-9 Y 33 Ethylene glycol 107-21-1 Y 674. FIRST AID MEASURESEYE CONTACT:Hold eyelids open and flush with a steady, gentle stream of water for at least 15 minutes. Seek medical attention if eye irritation develops or persists.SKIN CONTACT:Remove contaminated clothing and shoes. Wash with plenty of water, for at least 15minutes. Seek medical attention if skin irritation develops or persists. Launder contaminated clothing and shoes before re-use.SAFETY DATA SHEET: TEDA – L33E – US-GHS Version4. FIRST AID MEASURES (continued)INGESTION:Do not induce vomiting. If victim is conscious and alert, give 1-2 glasses of water to drink. Do not give anything by mouth to an unconscious person. Seek immediate medical attention. Do not leave victim unattended.INHALATION:If respiratory irritation or distress occurs, remove victim to fresh air. Seek imedical attention if respiratory irritation or drowsiness develops or persists.NOTES TO PHYSICIAN :All treatments should be based on observed signs and symptoms of distress in the patient. Consideration should be given to the possibility that overexposure to materials other than this product may have occurred. Treatsymptomatically. No specific antidote available.5. FIRE FIGHTING MEASURESEXTINGUISHING MEDIA: Water spray, fog, dry chemical, foam, CO 2UNUSUAL FIRE AND EXPLOSION HAZARDS:Closed containers may rupture due to buildup of pressure when exposed to extreme heat. SPECIAL PROTECTIVE EQUIPMENT FOR FIRE FIGHTERS:Firefighters should wear NIOSH/MSHA-approved self-contained breathing apparatus and full protective clothing. Cool containers exposed to fire with water.HAZARDOUS DECOMPOSITIONMATERIALS UNDER FIRE CONDITIONS : Oxides of carbon, oxides of nitrogen, ammonia.6. ACCIDENTAL RELEASE MEASURESPERSONAL PRECAUTIONS: Wear appropriate protective gear for thesituation. (See Personal Protection Information in Section 8).ENVIROMENTAL PRECAUTIONS :Do not flush to drain. Spills may be reportable to the National Response Center (800-424-8802) and to state and/or local agencies.METHOD FOR CLEAN UP:Extinguish or remove all sources of ignition. Absorb with an inert absorbent, sweep up and place in an appropriate closed container. Clean up residual material by washing area with water. Collect washings for disposal. Spills may be reportable to the National Response Center (800-424-8802) and to state and/or local agencies.SAFETY DATA SHEET: TEDA – L33E – US-GHS Version7. HANDLING AND STORAGEPRECAUTIONS FOR SAFE HANDLING : Handle material with suitable protection (SeeSection 8). Handle with adequate ventilation. Avoid breathing vapors. Avoid contact with eyes, skin and clothing.VENTILATION:General area dilution/exhaust ventilation.CONDITIONS FOR SAFE STORAGE :Store upright in a cool, dry, well ventilated area out of direct sunlight. Keep away from heat,open flames and ignition sources. Keep container tightly closed. Do not reuse container.8. EXPOSURE CONTROLS/PERSONAL PROTECTIONENGINEERING MEASURES:Set up hand-wash station and eyewash station near work area.General area dilution/exhaust ventilation.EXPOSURE LIMITS:Ethylene glycol – 100 mg/M 3 - ACGIH ceilingPERSONAL PROTECTION MEASURES:Respiratory protection :When respirators are required, selectNIOSH/MSHA approved equipment based on actual or potential airborne concentrations and in accordance with regulatory standards and/or industrial recommendations. Self-contained or supplied-air respiratory equipmment is recommended.Eye protection : Safety glasses with side shields, goggles or face shield are recommended.Skin protection :Skin contact should be minimized through the use of chemical-resistant gloves and boots, and suitable protective clothing.The following general measures should be taken when working or handling this material:1) Do not store, use, and/or consume foods, beverages, tobacco products, or cosmetics in areas where this material is stored.2) Wash hands and face carefully before eating, drinking, using tobacco, applying cosmetics, or using the toilet.3) Wash exposed skin promptly to remove accidental splashes of contact with this material.9. PHYSICAL AND CHEMICAL PROPERTIESPHYSICAL STATE: Liquid COLOR: Pale yellow ODOR: Ammonia-like pH: 11.0 (@10% aqueous) MELTING POINT: No data availableSAFETY DATA SHEET: TEDA – L33E – US-GHS Version9. PHYSICAL AND CHEMICAL PROPERTIES (continued)BOILING POINT: 363-385F (184-196C) FLASH POINT: 219F (104C) AUTOIGNITION POINT: 608F (320C) EXPLOSIVE LIMITS(Lower): No data available EXPLOSIVE LIMITS(Upper): No data available VAPOR PRESSURE: < 13 Pa @ 20C (68F) VAPOR DENSITY: 2.52 (Air = 1) EVAPORATION RATE: No data available RELATIVE DENSITY: 1.10 SOLUBILITY IN WATER : Soluble PARTITION COEFFICIENT: No data available DECOMPOSITION TEMPERATURE: No data available10. STABILITY AND REACTIVITYCHEMICAL STABILITY:This material is stable under normal handlingand storage conditions described in Section 7.CONDITIONS TO AVOID: Heat, open flame, sparks, direct sunlight.INCOMPATIBLE MATERIALS:Strong oxidizing agents, strong acids, copper, zinc, aluminum and their alloys.HAZARDOUS DECOMPOSITION PRODUCTS: Oxides of carbon, oxides of nitrogen, ammonia.HAZARDOUS POLYMERIZATION: Not applicable11. TOXICOLOGICAL INFORMATIONEYE CORROSION/IRRITATION: Severely irritating, rabbit. (Data for Triethylenediamine)SKIN CORROSION/IRRITATION: Moderately irritating, rabbit. (Data for Triethylenediamine)ACUTE TOXICITY:ACUTE ORAL TOXICITY: LD 50 = 1700 mg/kg, rat. (Data for Triethylenediamine)ACUTE DERMAL TOXICITY : LD 50 > 2000 mg/kg, rat. (Data for Triethylenediamine)ACUTE INHALATION TOXICITY :LC 50 ≥ 20.2 mg/L/1 hour, rat (tested as a 20% solution). (Data for Triethylenediamine)SKIN SENSITIZATIONNot a sensitizer (guinea pig). (Data for Triethylenediamine)GENETIC TOXICITYNot mutagenic in the Ames test or in vivo mouse micronucleus test. (Data for Triethylenediamine)SAFETY DATA SHEET: TEDA – L33E – US-GHS Version11. TOXICOLOGICAL INFORMATION (continued)CARCINOGENICITY:This product does not contain any substances that are considered by OSHA, NTP, IARC or ACGIH to be “probable” or “suspected” human carcinogens.REPRODUCTIVE TOXICITY:In a combined repeat-dose/reproductive study (OECD 422) with Triethylenediamine, theNOAEL (no-observed-adverse-effect level) for F0 reproductive toxicity was considered to be 300 mg/kg/day. The NOAEL for Fl neonatal toxicity was considered to be 300 mg/kg/day. The NOAEL for F0 parental systemic toxicity was considered to be 100 mg/kg/day.Reproductive studies with ethylene glycol show that in repeated dose toxicity studies, noevidence of an adverse impact on reproductive organs was observed. In special studies, including a three generation study in rats and continuous breeding protocols in mice, evidence of reproductive effects have been restricted to mice (but not rabbits or rats) exposed to doses considerably higher than those associated with developmental effects in this species or renal effects in rats.STOT-SINGLE EXPOSURE : Ethylene glycol may cause central nervous system depression and drowsiness.STOT-REPEATED EXPOSURE:In a combined repeat-dose/reproductive study (OECD 422) with Triethylenediamine, reversible, treatment-related effects wereobserved in the kidneys and bladders of mid-to-high dose animals. The NOAEL for ethylene glycol was determined to be 150 mg/kg/day and appears to be a threshold dose below which no renal toxicity occurs.12. ECOLOGICAL INFORMATIONECOTOXICITY:96hr LC 50 > 100 mg/L (carp)48hr EC 50 > 92 mg/L (daphnia magna)72hr EC 50 > 110 mg/L (algae, biomass), > 180mg/L (algae, growth rate) (All data for Triethylenediamine)PERSISTENCE AND DEGRADABILITY: Not readily biodegradable (Data forTriethylenediamine)MOBILITY IN SOIL:No data availableSAFETY DATA SHEET: TEDA – L33E – US-GHS Version13. DISPOSAL CONSIDERATION (INCLUDING CONTAINER)RESIDUAL WASTE:Chemical additions, processing or otherwise altering this material may make the waste management information presented in this MSDS incomplete, inaccurate or otherwiseinappropriate. Please be advised that state and local requirements for waste disposal may be more restrictive or otherwise different fromFederal laws and regulations. Consult state and local regulations regarding the proper disposal of this material.CONTAMINATED VESSELS AND CONTAINERS :Rinse containers before disposal. Do not allow rinsate to enter the water systems.EPA Hazardous Waste = No14. TRANSPORTATION INFORMATIONPROPER SHIPPING NAME: NOT REGULATED UN NUMBER:None UN CLASS or DIVISION: None UN PACKING GROUP: None LABELS :None EMERGENCY GUIDE#:None15. REGULATORY INFORMATIONInventory Status:US (TSCA): YesCanada (DSL): Yes EU (REACH): Yes Australia (AICS): Yes Japan (METI): Yes Korea (KECL): YesWhere: Yes = all ingredients are listed on the inventory, Exempt = All ingredients are either on the inventory or exempt from the requirements of listing, No = Not determined, or one or more ingredients are not on the inventory and are not exempt from listingSARA Title III Hazard Classes: Fire Hazard: No Reactive Hazard: No Release of Pressure: No Acute Health Hazard: Yes Chronic Health Hazard: YesSARA Extremely Hazardous Substances/CERCLA Hazardous Substances: Ethylene glycol (107-21-1) (33%), TPQ=5000 pounds, 2270 kgCalifornia Proposition 65: This product does not contain any components that are regulated under Proposition 65.SAFETY DATA SHEET: TEDA – L33E – US-GHS Version16. OTHER INFORMATION INCLUDING INFORMATION ON PREPARATION AND REVISION OF THIS MSDSNational Fire Protection Association (“NFPA”) Hazard Ratings: Health: 2 (Moderate)Flammability: 1 (Slight)Reactivity: 0 (Minimal)National Paint and Coatings Hazardous Materials Identification System (“HMIS”) Hazard Ratings: Health: 2 (Moderate)Flammability: 1 (Slight) Physical Hazard: 0 (Minimal)HISTORY: Date previous SDS: April 7, 2015 Date of issue: November 13, 2015 Reasons for Revision: Revised Phone NumberDisclaimer: The information set forth herein has been gathered from standard reference materials and/or TOSOH SPECIALTY CHEMICALS USA, INC and its related, subsidiary and affiliated companies’ test data and is to the best knowledge and belief of TOSOH SPECIALTY CHEMICALS USA, INC and its related, subsidiary and affiliated companies, accurate andreliable. Such information is offered solely for your consideration, investigation, and verification, and is not suggested or guaranteed that the hazard precautions or procedures mentioned are the only ones that exist. TOSOH SPECIALTY CHEMICALS USA, INC and its related, subsidiary and affiliated companies make no warranties, express or implied, and expressly disclaim any and all such warranties with respect to the use of such information or the use of specific materialidentified herein in combination with any other material or process, and assume no responsibility therefor. TOSOH SPECIALTY CHEMICALS USA, INC and its related, subsidiary and affiliated companies make no representation or warranty, express or implied, and EXPRESSLY DISCLAIM ANY AND ALL SUCH WARRANTIES, as to the usefulness, sufficiency, MERCHANTABILITY or FITNESS FOR ANY PURPOSE whatsoever of the materials identified herein. The purchaser bears sole responsibility for testing, evaluating and determining the suitability of these materials for whatever use(s), manufacturing and refining processes, and any other such application(s) for which it intends or ultimately makes of these materials. Purchaser bears sole responsibility for obtaining any and all regulatory, legal and governmental approval necessary for such use(s).END OF SAFETY DATA SHEETdistributed by:Request Quote or Samples。
2种不同试剂筛查无偿献血者HIV抗体结果分析
HIV抗体血清学检测是献血者是否感染艾滋病病毒的依据,目前我国采供血机构多采用酶联免疫吸附试验使用国产和进口试剂重复2次筛查献血员,结果可能有一定的差异,检测过程受到酶标板包被的抗原种类和数量、酶标板材料、检测人员、仪器、环境条件的不同影响时,会产生不同的检测结果;受标本溶血、污染、感染了与HIV抗原决定簇有交叉反应的传染病病原体等的影响,可能会产生假阳性结果。
为研究国产与进口试剂HIV抗体的初筛检测结果是否有差异性,通过对30125份相同的无偿献血者样本进行HIV抗体初筛检测比较,结果如下。
1 对象与方法1．1 对象对象为2010年1～12月无偿献血者样本30125份,留样后离心,吸取血清。
1．2 试剂北京万泰生物药业股份有限公司生产的第四代人类免疫缺陷病毒抗体诊断试剂盒和荷兰生物梅里埃有限公司生产的第四代人类免疫缺陷病毒抗体诊断试剂盒。
1．3 仪器瑞士Microlab FAME24/20全自动酶免分析仪及瑞士MICRO S T A R 8C H 全自动加样仪。
1．4 检查项目与标准血液筛查按卫生部《献血者健康检查标准》。
国产和进口试剂抗HIV -抗体EL IS A方法S/C O值≥1为阳性,S /C O值≤1为阴性,确认阳性以C D C 报告为准。
1.5 方法抗-HIV抗体筛查采用进口和国产试剂按照试剂盒的使用说明和注意事项进行操作和判断结果。
对初筛阳性结果再送检CDC 确认试验市确认。
2 结果2．1 试剂的灵敏度与特异性采用国家标准品的考核血清盘检测以上两种试剂的特异性与灵敏度,特异性均≥95%,灵敏度均为100%。
2.2 对30125份相同的无偿献血者样本进行HIV抗体初筛检测酶联免疫法初筛检测结果为阳性47例,阴性30078份,阳性率为0.16%,其中国产单种试剂初筛检测阳性25例,进口单种试剂阳性18例,国产与进口双试剂阳性4例,经送检CDC确认实验室确认有3例样本为抗-HIV抗体阳性。
Incucyte
Product Information Presentation, Storage and StabilityThe Incucyte® Fabfluor-pH Antibody Labeling Reagents for antibody internalization are supplied as lyophilized solids in sufficient quantity to label 50 μg of test antibody, when used at the suggested molar ratio (1:3 of test antibody to labeling Fab). The lyophilized solid can be stored at 2-8° C for one year. Once re-hydrated, any unused reagent should be aliquoted and stored at -80° C for up to one year. Avoid repeated freeze-thaw cycles.Incucyte® Fabfluor-pH Antibody Labeling ReagentsFor Antibody Internalization AssaysAntibody Labeling Reagent Rehydrated: -80° C *Excitation and Emission maxima were determined at a pH of 4.5.Fabfluor_quick_guideBackgroundIncucyte ® Fabfluor-pH Antibody Labeling Reagents are designed for quick, easy labeling of Fc-containing test antibodies with a Fab fragment-conjugated pH-sensitive fluorophore. The pH-sensitive dye based system exploits the acidic environment of the lysosomes to quantify in-ternalization of the labeled antibody. As Fabfluor labeled antibodies reside in the neutral extracellular solution (pH 7.4), they interact with cell surface specific antigens and are internalized. Once in the lysosomes, they enter an acidic environment (pH 4.5–5.5) and a substantial in-crease in fluorescence is observed. In the absence of ex-pression of the specific antigen, no internalization occurs and the fluorescence intensity of the labeled antibodies remains low. With the Incucyte ® integrated analysis soft-ware, background fluorescence is minimized. These reagents have been validated for use with a number of different antibodies in a range of cell types. The Incucyte ® Live-Cell Analysis System enables real-time, kinetic eval -uation of antibody internalization.Recommended UseWe recommend that the Incucyte ® Fabfluor-pH Antibody Labeling Reagents are prepared at a stock concentration of 0.5 mg/mL by the addition of 100 μL of sterile water and triturated (centrifuge if solution not clear). The reagent may then be diluted directly into the labeling mixture with test antibody. Do NOT sonicate the solution.Additional InformationThe Fab antibody was purified from antisera by a combination of papain digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.Human Red (Cat. No. 4722) or Human Orange (Cat. No. 4812)—Based on immunoelectrophoresis and/ or ELISA, the antibody reacts with the Fc portion of human IgG heavy chain but not the Fab portion of human IgG. No antibody was detected against human IgM, IgA or against non-immunoglobulin serum proteins. The anti-body may cross-react with other immunoglobulins from other species.Mouse IgG1 (Cat. No. 4723), IgG2a (Cat. No. 4750) or IgG2b (Cat. No. 4751)—Based on antigen-binding assay and/or ELISA, the antibody reacts with the Fc portion of mouse IgG, IgG2a or IgG2b, respectively, but not the Fab portion of mouse immunoglobulins. No antibody was detected against mouse IgM or against non–immunoglobulin serum proteins. The antibody may cross-react with other mouse IgG subclasses or with immunoglobulins from other species.Rat (Cat. No. 4737)—Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of rat IgG heavy chain but not the Fab portion of rat IgG. No antibody was detected against rat IgM, IgA or against non-immunoglobulin serum proteins. The antibody may cross-react with other immunoglobulins from other species.A.B.C.D.R e d O b j e c t A r e a (x 105 μm 2 p e r w e l l )Time (hours)A U C x 106 (0–12 h )log [α–CD71] (g/mL)Example DataFigure 1: Concentration-dependent increase in antibody internalization of Incucyte ® Fabfluor labeled-α-CD71 in HT1080 cells. α-CD71 and mouse IgG1 isotype control were labeled with Incucyte ® Mouse IgG1 Fabfluor-pH Red Antibody Labeling Reagent. HT1080 cells were treated with either Fabfluor-α-CD71 or Fabfluor-IgG1 (4 μg/mL); HD phase and red fluorescence images were captured every 30 minutes over 12 hours using a 10X magnification. (A) Images of cells treated with Fabfluor-α-CD71 display red fluorescence in the cytoplasm (images shown at 6 h). (B) Cells treated with labeled isotype control display no cellular fluorescence. (C) Time-course of Fabfluor-α-CD71 internalization with increasing concentrations of Fabfluor-α-CD71 (progressively darker symbols). Internalization has been quantified as the red object area for each time-point. (D) Concentration response curve to Fabfluor-α-CD71. Area under the curve (AUC) values have been determined from the time-course shown in panel C (0-12 hours) and are presented as the mean ± SEM, n=3 wells.CD71-FabfluorIgG-FabfluorProtocols and ProceduresMaterialsIncucyte® Fabfluor-pH Antibody Labeling ReagentTest antibody of interest containing human, mouse, or rat IgG Fc region (at known concentration)Target cells of interestTarget cell growth mediaSterile distilled water96-well flat bottom microplate (e.g. Corning Cat. No. 3595) for imaging96-well round black round bottom ULA plate (e.g. Corning Cat. No. 45913799) or amber microtube (e.g. Cole Parmer Cat. No. MCT-150-X, autoclaved) for conjugation step0.01% Poly-L-Ornithine (PLO) solution (e.g. Sigma Cat. No. P4957), optional for non-adherent cells Recommended control antibodiesIt is strongly recommended that a positive and negative control is run alongside test antibodies and cell lines. For example, CD71, which is a mouse anti-human antibody, is recommended as a positive control for the mouse Fab.Anti-CD71, clone MEM-189, IgG1 e.g. Sigma Cat. No. SAB4700520-100UGAnti-CD71, clone CYG4, IgG2a e.g. BioLegend Cat. No. 334102Isotype controls, depending on isotype being studied—Mouse IgG1, e.g. BioLegend Cat. No. 400124, Mouse IgG2a e.g. BioLegend Cat. No. 401501Preparation of Incucyte® Antibody Internalization Assay 1. Seed target cells of interest1.1 Harvest cells of interest and determine cell concentra-tion (e.g. trypan blue + hemocytometer).1.2 Prepare cell seeding stock in target cell growth mediawith a cell density to achieve 40–50% confluence be-fore the addition of labeled antibodies. The suggested starting range is 5,000–30,000 cells/well, although the seeding density will need to be optimized for each cell type.Note: For non-adherent cell types, a well coating may be required to maintain even cell distribution in the well. For a 96-well flat bottom plate, we recommend coating with 50 μL of either 0.01% Poly-L-Or-nithine (PLO) solution or 5 μg/mL fibronectin diluted in 0.1% BSA.Coat plates for 1 hour at ambient temperature, remove solution from wells and then allow the plates to dry for 30-60 minutes prior to cell addition.1.3 Using a multi-channel pipette, seed cells (50 µL perwell) into a 96-well flat bottom microplate. Lightly tapplate side to ensure even liquid distribution in well. Toensure uniform distribution of cells in each well, allowthe covered plate sit on a level surface undisturbed at room temperature in the tissue culture hood for 30minutes. After cells are settled, place the plate insidethe Incucyte® Live-Cell Analysis System to monitor cell confluence.Note: Depending on cell type, plates can be used in assay once cells have adhered to plastic and achieved normal cell morphology e.g.2-3 hours for HT1080 or 1-2 hours for non-adherent cell types. Some cell types may require overnight incubation.2. Label Test Antibody2.1 Rehydrate the Incucyte® Fabfluor-pH Antibody Label-ing Reagent with 100 µL sterile water to result in a final concentration of 0.5 mg/mL. Triturate to mix (centrifuge if solution is not clear).Note: The reagent is light sensitive and should be protected fromlight. Rehydrated reagent can be aliquoted into amber or foilwrapped tubes and stored at -80° C for up to 1 year (avoid freezing and thawing).2.2 Mix test antibody with rehydrated Incucyte® Fabfluor–pH Antibody Labeling Reagent and target cell growth media in a black round bottom microplate or ambertube to protect from light (50 µL/well).a. Add test antibody and Incucyte® Fabfluor–pH Anti-body Labeling Reagent at 2X the final concentration.We suggest optimizing the assay by starting with afinal concentration of 4 µg/mL of test antibody or theFabfluor-pH Antibody Labeling Reagent (i.e. 2Xworking concentration = 8 µg/mL).Note: A 1:3 molar ratio of test antibody to Incucyte® Fabfluor-pHAntibody Labeling Reagent is recommended. The labeling re-agent is a third of the size of a standard antibody (50 and 150KDa, respectively). Therefore, labeling equal quantities will pro-duce a 1:3 molar ratio of test antibody to labeling Fab.b. Make sufficient volume of 2X labeling solution for50 µL/well for each sample. Triturate to mix.c. Incubate at 37° C for 15 minutes protected from light.Note: If performing a range of concentrations of test antibody,e.g. concentration response-curve, it is recommended to createthe dilution series post the conjugation step to ensure consistentmolar ratio. We strongly recommend the use of both a negativeand positive control antibody in the same plate.3. Add labeled antibody to cells3.1 Remove cell plate from incubator.3.2 Using a multi-channel pipette, add 50 µL of 2X labeledantibody and control solutions to designated wells.Remove any bubbles and immediately place plate in the Incucyte® Live-Cell Analysis System and start scanning.Note: To reduce the risk of condensation formation on the lid priorto first image acquisition, maintain all reagents at 37° C prior toplate addition.4. Acquire images and analyze4.1 In the Incucyte® Software, schedule to image every15-30 minutes, depending on the speed of the specific antibody internalization.a Scan on schedule, standard. If the Incucyte® Cell-by-Cell Analysis Software Module (Cat. No. 9600-0031)is available, adherent cell-by-cell or non-adherentcell-by-cell scan types can be selected.b Channel selection: select “phase” and “red” or“phase” and "orange” (depending on reagent used).c Objective: 10X or 20X depending on cell types used,generally 10X is recommended for adherent cells,and 20X for non-adherent or smaller cells.NOTE: The optional Incucyte® Cell-by-Cell Analysis SoftwareModule enables the classification of cells into sub-populationsbased on properties including fluorescence intensity, size andshape. For further details on this analysis module and its appli-cation, please see: /cell-by-cell.4.2 To generate the metrics, user must create an AnalysisDefinition suited to the cell type, assay conditions andmagnification selected.4.3 Select images from a well containing a positiveinternalization signal and an isotype control well(negative signal) at a time point where internalizationis visible.4.4 In the Analysis Definition:Basic Analyzer:a. Set up the mask for the phase confluence measurewith fluorescence channel turned off.b. Once the phase mask is determined, turn the fluores-cence channel on: Exclude background fluorescencefrom the mask using the background subtractionfeature. The feature “Top-Hat” will subtract localbackground from brightly fluorescent objects withina given radius; this is a useful tool for analyzing ob-jects which change in fluorescence intensity overtime.i The radius chosen should reflect the size of thefluorescent object but contain enough backgroundto reliably estimate background fluorescence inthe image; 20-30 μm is often a useful startingpoint.ii The threshold chosen will ensure that objectsbelow a fluorescence threshold will not bemasked.iii Choose a threshold in which red or orange objectsare masked in the positive response image but lownumbers in the isotype control, negative responsewell. For a very sensitive measurement, for example,if interested in early responses, we suggest athreshold of 0.2.NOTE: The Adaptive feature can be used for analysis but maynot be as sensitive and may miss early responses. If interestedin rate of response, Top-Hat may be preferable.Cell-by-Cell (if available):a. Create a Cell-by-Cell mask following the softwaremanual.b. There is no need to separate phase and fluorescencemasks. The default setting of Top-Hat No Mask forthe fluorescence channel will enable backgroundsubtraction without generation of a mask. Ensurethat the Top-Hat radius is set to a value higher thanthe radius of the larger clusters to avoid excess back-ground subtraction.c. The threshold of fluorescence can be determined inCell-by-Cell Classification.Specifications subject to change without notice.© 2020. All rights reserved. Incucyte, Essen BioScience, and all names of Essen BioScience prod -ucts are registered trademarks and the property of Essen BioScience unless otherwise specified. Essen BioScience is a Sartorius Company. Publication No.: 8000-0728-A00Version 1 | 2020 | 04Sales and Service ContactsFor further contacts, visit Essen BioScience, A Sartorius Company /incucyte Sartorius Lab Instruments GmbH & Co. KGOtto-Brenner-Strasse 20 37079 Goettingen, Germany Phone +49 551 308 0North AmericaEssen BioScience Inc. 300 West Morgan Road Ann Arbor, Michigan, 48108USATelephone +1 734 769 1600E-Mail:***************************EuropeEssen BioScience Ltd.Units 2 & 3 The Quadrant Newark CloseRoyston Hertfordshire SG8 5HLUnited KingdomTelephone +44 (0) 1763 227400E-Mail:***************************APACEssen BioScience K.K.4th floor Daiwa Shinagawa North Bldg.1-8-11 Kita-Shinagawa Shinagawa-ku, Tokyo 140-0001 JapanTelephone: +81 3 6478 5202E-Mail:*************************5. Analysis GuidelinesAs the labeled antibody is internalized into the acidic environment of the lysosome, the area of fluorescence intensity inside the cells increases.This can be reported in two ways:Ways to Report Basic AnalyzerCell-by-Cell Analysis* To correct for cell proliferation, it is advisable to normalize the fluorescence area to the total cell area using User Defined Metrics.For Research Use Only. Not For Therapeutic or Diagnostic Use.LicensesFor non-commercial research use only. Not for therapeutic or in vivo applications. Other license needs contact Essen BioS cience.Fabfluor-pH Red Antibody Labeling Reagent: This product or portions thereof is manufactured under license from Carnegie Mellon University and U.S. patent numbers 7615646 and 8044203 and related patents. This product is licensed for sale only for research. It is not licensed for any other use. There is no implied license hereunder for any commercial use.Fabfluor-pH Orange Antibody Labeling Reagent: This product or portions thereof is manufactured under a license from Tokyo University and is covered by issued patents EP2098529B1, JP5636080B2, US8258171, and US9784732 and related patent applications. This product and related products are trademarks of Goryo Chemical. Any application of above mentioned technology for commercial purpose requires a separate li -cense from: Goryo Chemical, EAREE Bldg., SF Kita 8 Nishi 18-35-100, Chuo-Ku, Sapporo, 060-0008 Japan.SupportA complete suite of cell health applications is available to fit your experimental needs. Find more information at /incucyte Foradditionalproductortechnicalinformation,************************************************************/incucyte。
免疫组化实验--全套试剂耗材
免疫组化实验试剂耗材大全华越洋---------------------------- 0.1%胰蛋白酶消化液waryong 10ml 110多聚甲醛merk 25g 504%多聚甲醛waryong 500ml 22010X多聚赖氨酸waryong 10ml 260抗荧光衰减封片剂waryong 25ml 230防脱载玻片waryong 50片310mayer'苏木素染液（免疫组化）waryong 100ml 410封闭用正常绵羊/山羊/兔/人血清waryong 10ml 75弗氏不完全佐剂sigma 10ml 180弗氏完全佐剂sigma 10ml 200柠檬酸钠缓冲液0.01mol/L PH6.0 waryong 1L 10DAB amresco 1g 13520XDAB显色液 A，B液各1.5ml waryong 3ml 95NBT amresco 100mg 95BCIP amresco 100mg 310BCIP/NBT底物显色试剂盒waryong 25ml 210PBST(PH7.4)抗体稀释液waryong 1ml 25一抗稀释液waryong 100ml 390HRP标记抗体稀释液waryong 100ml 390AP标记抗体稀释液waryong 100ml 390荧光抗体稀释液waryong 50ml 110免疫组化名称规格价格Super Polymer-二步法IHC试剂盒3ml35818ml1598兔Streptavidin-HRP试剂盒3ml19818ml998鼠Streptavidin-HRP试剂盒3ml19818ml998兔∕鼠通用型Streptavidin-HRP试剂盒3ml25818ml1198山羊抗兔IgG,Biotin(IHC工作液)3ml6818ml298山羊抗鼠IgG,Biotin(IHC工作液)3ml6818ml298山羊抗兔∕鼠IgG,Biotin(IHC工作液)3ml9818ml498 Streptavidin-HRP(IHC工作液)3ml9818ml49860ml258 AEC底物显色试剂盒20ml98 BCIP∕NBT碱性磷酸酶显色试剂盒(40x)40ml198 BCIP/NBT碱性磷酸酶显色试剂40ml229改良型苏木素(IHC常用复染试剂)10ml68柠檬酸缓冲液(IHC抗原修复液,100x)100ml68EDTA缓冲液(IHC抗原修复液，50x)100ml68封闭用正常山羊血清工作液(免疫组化封闭液)10ml68内源性过氧化物酶封闭液10ml68内源性碱性磷酸酶封闭液10ml68Biotin标记抗体稀释液20ml中性树胶100g98水性封片剂10ml98 Super Polymer-二步法IHC试剂盒（带DAB显色液）3ml39818ml1698兔Streptavidin-HRP试剂盒（带DAB显色液）3ml25818ml1098鼠Streptavidin-HRP试剂盒（带DAB显色液）3ml25818ml1098兔∕鼠通用型Streptavidin-HRP试剂盒（带DAB显色液）3ml29818ml1298。
Sigma-Aldrich实验室常用生化试剂大促销
缓冲液
产品货号 英文品名 中文品名 优惠价 (R M B ) 目录价 (RMB)
A1542-2.5KG A1542-250G A1542-500G B7901-1KG B7901-500G C3041-100CAP C3041-50CAP C3674-100G C3674-1KG C3674-500G E9508-100ML E9508-10UL E9508-1L E9508-2.5L E9508-500ML E6758-100G E6758-500G H3375-100G H3375-1KG H3375-250G H3375-25G H3375-500G H3375-5KG I0125-100G I0125-10G I0125-1KG I0125-25G I0125-500G I0125-5KG M2933-100G M2933-1KG M2933-25G M2933-500G M1254-100G M1254-1KG M1254-250G M1254-25G M1254-50KG M1254-5KG P5493-1L P5493-4L P4809-100TAB P4809-50TAB
Agarose Agarose Agarose Agarose Agarose Agarose Agarose Agarose Agarose Agarose Agarose Agarose
低熔点琼脂糖 低熔点琼脂糖 低熔点琼脂糖 低熔点琼脂糖 低熔点琼脂糖 低熔点琼脂糖 琼脂糖 琼脂糖 琼脂糖 琼脂糖 琼脂糖 琼脂糖
Ammonium acetate ~98% Ammonium acetate ~98% Ammonium acetate ~98% Boric acid Boric acid Carbonate-Bicarbonate Buffer Carbonate-Bicarbonate Buffer Citric acid trisodium salt Citric acid trisodium salt Citric acid trisodium salt Ethanolamine >=98% Ethanolamine >=98% Ethanolamine >=98% Ethanolamine >=98% Ethanolamine >=98% Ethylenediaminetetraacetic acid >=98.5% Ethylenediaminetetraacetic acid >=98.5% HEPES >=99.5% (titration) HEPES >=99.5% (titration) HEPES >=99.5% (titration) HEPES >=99.5% (titration) HEPES >=99.5% (titration) HEPES >=99.5% (titration) Imidazole >=98.5% (titration) Imidazole >=98.5% (titration) Imidazole >=98.5% (titration) Imidazole >=98.5% (titration) Imidazole >=98.5% (titration) Imidazole >=98.5% (titration) MES hydrate >=99.5% MES hydrate >=99.5% MES hydrate >=99.5% MES hydrate >=99.5% MOPS >=99.5% (titration) MOPS >=99.5% (titration) MOPS >=99.5% (titration) MOPS >=99.5% (titration) MOPS >=99.5% (titration) MOPS >=99.5% (titration) Phosphate buffered saline Phosphate buffered saline Phosphate-Citrate Buffer Phosphate-Citrate Buffer
他莫昔芬協同Ad-p53治疗乳腺癌的实验研究
中英文缩略词英文缩写英文全称中文全称Ad-p53 recombinant adenovirus mediated p53 gene 重组人p53腺病毒TAM tamoxifen 他莫昔芬SDS sodium dodecyl sulfonate 十二烷基磺酸钠SDS-PAGE sodium dodecyl sulfate polyacrylamide 聚丙烯酰胺凝胶电泳gel electrophoresisDMEM dulbecco's modified eagle medium DMEM改良培养基FBS fetal bovine serum 胎牛血清PBS phosphate buffered saline 磷酸盐缓冲液GAPDH glyceraldehyde-3-phosphate dehydrogenase 甘油醛-3-磷酸脱氢酶VP virus particle 病毒颗粒PFU plaque forming unit 空斑形成单位MOI multiplicity of infection 感染复数MCF-7 Michigan Cancer Foundation – 7 密歇根癌症基金会-7细胞系PMSF phenylmethanesulfonyl fluoride 苯甲基磺酰氟RIPA Lysis Buffer radio immunoprecipitation assay 放射免疫沉淀法裂解液lysis bufferBCA bicinchoninic acid 2,2-联喹啉-4,4-二甲酸二钠PVDF poly(vinylidene fluoride) 聚偏氟乙烯TBS Tris buffered saline Tris盐酸缓冲液TBST Tris buffered saline-Tween 20 Tris盐酸-Tween20缓冲液ECL electrochemiluminescence 化学发光试剂mL milliliter 毫升μL microlitre 微升uM- micromolar/litre 微摩尔/升mol/L molar/litre 摩尔/升mg microgram 毫克h hour 小时V volt 伏OD optical density 光密度PI propidium iodide 碘化丙啶RPM revolutions per minute 转每分EDTA Ethylene Diamine Tetraacetic Acid 依地酸(乙二胺四乙酸)2他莫昔芬协同Ad-p53治疗乳腺癌的实验研究中文摘要研究目的p53是重要的抑癌基因，其突变或失活与肿瘤的发生发展关系密切，目前认为将具有正常功能的抑癌基因导入肿瘤细胞内，可以恢复突变或失活基因的功能。
Tris-Tricine-SDS-PAGE凝胶制备试剂盒使用说明
Tris-Tricine-SDS-PAGE凝胶制备试剂盒使用说明货号：P1320规格：25块/50块/100块PAGE凝胶产品内容：25T50T100T保存条件49.5%T3%C15ml30ml60ml2-8℃，避光49.5%T6%C50ml100ml200ml2-8℃，避光凝胶缓冲液70ml140ml280ml2-8℃50%甘油30ml60ml120ml室温APS0.25g0.5g 1.0g室温TEMED400ul750ul 1.5ml室温，避光说明书一份一份一份本产品所提供的APS（过硫酸铵）为固体粉末，使用前加入双蒸水溶解即配制成10%APS 溶液（0.5g APS5ml双蒸水），将溶液分装后置于-20℃保存，通常半年内有效。
溶液在使用中可放置4℃保存两周。
产品简介：常规的Tris-SDS-PAGE电泳的只能分辨大分子蛋白，对于相对分子量小的，尤其是10kD以下的蛋白分辨率极低。
而Tricine-SDS-PAGE可以很好的分离分子量在1-10kD的蛋白及多肽，成为目前电泳法变性分离多肽的主要方法。
本产品包括Tricine-SDS-PAGE凝胶制备所需全套试剂，只需自备蒸馏水，即可制备高质量各种浓度的凝胶，方便、快捷，电泳后可直接用于考染、银染、Western杂交等实验。
注意事项：1、10%APS配制后分装-20度保存。
APS溶液不稳定，应尽量减少室温存放时间，每次取用后立即放回冰箱，以防失效；若发现凝胶聚合时间延长，应考虑更换，使用-20度保存的10%APS。
2、在凝胶配制过程中，尤其是液体混匀步骤，应尽量避免气泡的产生。
3、在分离胶上层加蒸馏水时要小心操作，加水时速度不能太快。
4、丙烯酰胺具有神经毒性，操作时请穿着实验服并佩戴一次性手套。
5、本产品仅用于科研，不能用于人体实验或人体治疗。
配胶说明：分离胶夹层胶浓缩胶20%/4.5ml16.5%/4.5ml15.5%/4.5ml10%/2ml4%/2ml 49.5%T3%C///0.407ml0.160ml 49.5%T6%C 1.82ml 1.50ml 1.395ml//凝胶缓冲液 1.50ml 1.50ml 1.50ml0.667ml0.496ml 50%甘油0.96ml0.96ml0.96ml//ddH2O0.22ml0.54ml0.645m0.926ml 1.344ml 10%APS40µl40µl40µl20µ|20µ| TEMED5µl5µl5µl3µl3µl根据目的蛋白分子量大小选择合适的PAGE分离胶配制浓度，凝胶浓度配方参考附表。