microrna-136-5p靶向stat3调控对脊髓损伤大鼠炎症因子的影响

文献标识码 ： A
Effects of STAT3 targeting by microRNA136-5p on inflammatory factors in rats with spinal cord injury*
Nan Wu1, Tao Wang1, Jian-xiong Ma2, Gong-yi Lü1, Xin-long Ma3 (1. Department of Spinal Surgery, Tianjin Hospital, Tianjin, 300211, China; 2. Tianjin Orthopedic
收稿日期 ：2019-05-07 * 基金项目 ：天津市卫生行业重点攻关项目（No ：16KG140）
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中国现代医学杂志
第 29 卷
behavioral score (CBS). The expressions of interleukin-6 (IL-6), interleukin-21 (IL-21), interleukin-23 (IL-23) and the relative expressions of STAT3, phosphorylated STAT3 (p-STAT3) protein and IL-17 mRNA in spinal cord of rats in four groups were detected. Results BBB score of silent group was higher than that of spinal cord injury group and overexpression group (P < 0.05); CBS score of silent group was lower than that of spinal cord injury group and overexpression group (P < 0.05). The expression levels of IL-6, IL-21 and IL-23 in spinal cord tissue of rats in silence group were lower than those in spinal cord injury group and over-expression group (P < 0.05). The relative expressions of STAT3, p-STAT3 and IL-17 in spinal cord tissue of silent rats were lower than those of control group, spinal cord injury group and over-expression group (P < 0.05). Conclusion The silencing of miR-136-5p after spinal cord injury can inhibit the expression of STAT3, and then inhibit the overexpression of inflammatory factors, and ultimately inhibit the inflammatory response of spinal cord.
Research Institute, Tianjin, 300050, China; 3. Department of Osteology, Tianjin Hospital, Tianjin, 300211, China)
Abstract: Objective To investigate the effects of microRNA-136-5p (miR-136-5p) targeting signal transducers and transcriptional activators 3 (STAT3) on inflammatory factors in rats with spinal cord injury. Methods Sixty SD healthy rats were randomly divided into control group, spinal cord injury group, silent group and overexpression group. Spinal cord injury group, silent group and over-expression group were used to establish spinal cord injury model. The control group was not treated during the modeling period. The lentiviral suspension of 10 μl miR136-5p (viral titer 108 TU/ml) was injected into the spinal cord injury area of rats in silence group and overexpression group, and the rats in control group and spinal cord injury group were injected with the same amount of saline. The motor function and spinal nerve function of rats in four groups were evaluated by BBB score and combined
基础研究·论著
MicroRNA-136-5p 靶向 STAT3 调控 对脊髓损伤大鼠炎症因子的影响 *
吴楠 1，王涛 1，马剑雄 2，吕工一 1，马信龙 3
（1. 天津医院 脊柱外科，天津 300211 ；2. 天津市中西医结合骨科研究所，天津 300050 ； 3. 天津医院 骨外科，天津 300211）
对照组、脊髓损伤组大鼠注射等量的生理盐水。使用 BBB 评分、联合行为评分法（CBS）对 4 组大鼠运动功能、
脊髓神经功能进行评价，检测 4 组大鼠脊髓组织白细胞介素 -6（IL-6）、白细胞介素 -21（IL-21）、白细胞介素
-23（IL-23）表达水平及 STAT3、磷酸化 STAT3（p-STAT3）蛋白相对表达量，以及 IL-17 mRNA 表达水平。
结果 沉默组大鼠 BBB 评分于脊髓损伤组和过表达组（P <0.05）；沉默组大鼠 CBS 评分低于脊髓损伤组和过
表达组（P <0.05）。沉默组大鼠脊髓组织 IL-6、IL-21、IL-23 表达水平低于脊髓损伤组和过表达组（P <0.05）。
沉默组大鼠脊髓组织 STAT3、p-STAT3 蛋白相对表达量、IL-17 mRNA 表达水平均低于对照组、脊髓损伤组
摘要 ：目的 研究 microRNA-136-5p（miR-136-5p）靶向信号转导和转录激活子 3（STAT3）调控对脊
髓损伤大鼠炎症因子的影响。方法 选取 60 只 SD 健康大鼠，随机分为对照组、脊髓损伤组、沉默组及过表达组，
每组 15 只。将脊髓损伤组、沉默组、过表达组大鼠复制脊髓损伤模型，对照组在模型复制期间不做任何处理。 无菌环境下将 10 μl miR-136-5p 慢病毒悬液（病毒滴度为 108 TU/ml）注射于沉默组、过表达组大鼠脊髓损伤区。
第 29 卷 第 23 期 2019 年 12 月
中国现代医学杂志
China Journal of Modern Medicine
Vol. 29 No.23 Dec. 2019
DOI: 10.3969/j.issn.1005-8982.2019.23.001 文章编号： 1005-8982（2019）23-0001-06
及过表达组（P <0.05）。结论 脊髓损伤后 miR-136-5p 沉默可抑制 STAT3 的表达，进而抑制炎症因子的过度表
达，最终抑制脊髓炎症反应。
关键词 ： 脊髓损伤 ；microRNA-136-5p/MicroRNAs ；信号转导和转录激活子 3 ；炎症因子
中图分类号 ： R651.2