An Attributional Analysis of Resistance to Group Pressure Regardi[1]

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hallmarks of cancer-the next generation

hallmarks of cancer-the next generation
Sustaining Proliferative Signaling Ar可gu论ab证ly地the most fundamental trait of cancer cells involves their ability to sustain chronic proliferation. Normal tissues carefully control the production and release of growth-promoting signals that i指ns导truct entry into and progression through the cell growthand-division cycle, thereby ensuring a homeostasis of cell
We noted as an a辅nc助illa的ry p提rop议os,iti建on议that tumors are more than in孤su立la的r masses of pro增lif值er的ati癌ng细ca胞ncer cells. Instead, they are complex tissues composed of multiple distinct cell types that participate in h异ete形ro的typic interactions with one another. We depicted the recruited normal cells, which form tumor-associated stroma, as active participants in tumorigenesis rather than p被as动siv的e b旁ys观tan者ders; as such, these stromal cells contribute to the development and expression of certain hallmark capabilities. During the ensuing decade this notion has been solidified and extended, revealing that the biology of tumors can no longer be understood simply by en列um举erating the traits of the cancer cells but instead must encompass the contributions of the ‘‘tumor microenvironment’’ to tumorigenesis.

A practical scale evaluation of catalysts for the selective reduction of NOx with

A practical scale evaluation of catalysts for the selective reduction of NOx with

temperature range, selectivity to N20 and emission of non-reacted or incompletely oxidized reductants, were similar to that shown when model gas mixture systems were used. 0 1998 Elsevier Science B.V.
Keywords:
Diesel exhaust; Nitrogen oxide
1. Introduction Since 1990, there have been a large number of reports about the selective catalytic reduction of nitrogen oxides (NO,) with organic substances under netoxidizing conditions. Many of these were aiming at finding the best catalysts, reductants and catalystreductant combinations. For example, at the annual meetings of the Chemical Society of Japan and that of the Catalytic Society of Japan from 1990 to 1995, more than 300 kinds of catalytic systems have been
Akira Obuchi*, Isamu Kaneko, Junk0 Oi, Akihiko Ohi, Atsushi Ogata, Gratian R. Bamwenda, Satoshi Kushiyama

拉康的理论——精选推荐

拉康的理论——精选推荐

. 黄华,权利,身体与自我一福科与女性主义文学批评「M]北京:北京大学出版社,2005必须注意的是,自我的建构是在一种时间的辩证法中实现的。

对拉康来说,镜子阶段远不仅是幼儿的一个自然发展阶段,而且是主体发展中一个体现了时间辩证法的、先行和回溯相互交织的决定性的时刻。

“这一发展是作为时间的辩证法而经历的,它把个体的形成决定性地投射进历史之中:镜子阶段是一出戏,其内在压力迅猛地从不足冲向先行(anticipation)——对于受空间认同诱惑的主体而言,这出戏生产了从身体的破碎形象到我所说的关于身体整体性的外科整形形式的种种幻想——直到最终穿戴起异化身份的盔甲。

这副盔甲以其僵硬的结构标识出主体的精神发展。

”[2](p6)拉康认为,镜子有一个整体的功能,构建统一的自我,自我形成于镜像阶段。

来源于作者与自我分裂 6Lacan and His Fundamental Concepts of Theory of SubjectJacques Lacan(1901-80)is undoubtedly the central figure of psychoanalysis in the second half of the 20th century.He has notonly revolutionized the psychoanalytic practice but also exerted a global reinterpretation of the entire psychoanalytic theoretical edificeby employing the achievements of structural linguistics and semiotics.The reinterpretation has changed the entire field of the scientific debate.Some of his formulas such as“[T]he unconscious is structured like a language”;“[D]esire is thedesire of the Other”(Ecrites:A Selection 121,123),etc.acquire an almost iconic statuslike Einstein’s E=mc2.The least one can say about Lacan is that nobody was undisturbed and unaffected by his work:even those whopassionately oppose him have to take stance from his theory. ForLacan, psychoanalysis totally changes the way we should understand“cause”,and“reality”,which the fundamental notions like “subject’,clearly indicates that the Freudian unconscious as primordial and irrational drives is ridiculous; on the contrary, the unconscious is in aspecific way fully rational, discursive, “structured likea language”(Ecrites:A Selection 121),which entails that Lacanreduces all psychic lifeto a symbolic interplay. He proposes the triangle of Imaginary-Symbolic-Real as the three basic phases of forming the subject,the elementary matrix of the human experience.The term Imaginary is obviously similar with fiction but in Lacaniansense it is notsimply synonymous with the fictional or unreal;on the contrary,imaginary identifications can have very real effects. Thereis of course an air of unreality to the Imaginary: it traps the subjectinto alienating identifications that prevent the truth from emerging.In the Mirror Stage that is a key step to the formation of the subject,when the baby at the age from six to eighteen months glimpses its own image in a mirror or some equivalent to a mirror, itgreets the image in the mirror with great jubilation and thus identifies with it which is unreal by definition and shows somethingyet to become: an integrated unity. It is this identification that forms its unconscious, but its initial self-identification is inevitably basedon the Other; so identification with this image in the mirror means alienation of the subject. Therefore, from the beginning, the site of the subject is occupied by the other; the subject is in fact deteriorated into emptiness in essence. The unconscious which is the soul of theunconscious, while the subject becomes accordingly the other’sdesire of the subject is the desire of the other. In this sense, the unconscious is not a latent being since it is not an entity; on the contrary, it is rather a negativity, a lack of being, and a hole in chains of signifiers. Lacan calls it pre-ontological. Strictly speaking, the unconscious cannot be defined in the sense of being delimited.The Symbolic is a register that exists prior to the individual subject, into which the subject must be inserted if he/she is to be able to speak and desire. The Symbolic is often exhibited as languages, cultures, persons and surroundings around. Insertion into the Symbolic is a negative or privative process which implies recognition on the part of the child that it is not in possession of thedesire. It is also an object phallus which is the object of the mother’swith which the child attempts to identify (desire always beinga desire of and for the Other).The agency that imposes the law and deprives the child of the phallus in an act of symbolic castration isthe name of father. It is in the name of father that we recognize the symbolic function which has identified the person with the figure ofthe law. In other words, the name of father is the sign of law.Insertion into the Symbolic thus implies renunciation of the illusionof omnipotence associated with identification with the phallus, and italso introduces a split into the subject. The subject, as used by Lacan,is a deliberately ambiguous term; it refers to both the subject of a sentence and the subject of the impersonal laws of language. The subject is always divided or decentered, not simply because of the presence of the unconscious, but because of the structures of language itself. “Existence i s a product of language”(Xie Qun:24). For a subject,to be given a signifier or named is of vital importance.Thesymbolic/language decides the existence of being.The things that enter the Symbolicand are represented by language become existent. The things unnamed by language will not exist into being.By analogy,a subjectthat is represented is recognized as a being.A child has to allow itselfto be represented by language/signifier in order to be a subject.Losing the mark of a signifier,one becomes non-being.Although the Symbolic differentiates dimensions that are confusedin the Imaginary, it does not replace or transcend the Imaginary.Though every subject must be inserted into or inscribed in the Symbolic,no subject lives in the Symbolic alone, just as speech doesnot replace imagery. The two orders co-exist with a third: the Real. Itis not synonymous with external reality but equal to the left dimension that constantly resists symbolism and signification. Thetwo conceptions the Real and the reality are mutually exclusive.the daily life-world in whichWhat we experience as “reality”―we feel athome—can only stabilize itself through the exclusion or primordial repression of the traumatic Real, while the Real is often in the guiseof fantastic apparitions which forever haunt the subject. It is the threatening element that invades the subject when rifts appear in the Symbolic, and the failure to recognize or submit to the name of thefather opens up such rifts. The implication of Lacan’s theory may beworks. First, the mirror stagetwo-fold in analyzing Joseph Conrad’sinitial attempt to construct an identity as anmarks the child’sindependent entity from the Other and to locate a position in the society. Since its self-identification is based on an idealized unitaryimage, fragmentation and loss underline its experience as a subjectonce it steps into the register of Symbolic .The characters in theworks are haunted by severe loss and inner split when they experience what differentiates its prior existential conditions.Second,the implication of Lacan’stheory is found in its explanation of thecauses and destination of human desires and how they are related tobecome a subject. Lacan emphasizes that man’s d esire is theand the desire aims at the phallus, in accessible tomother’s desire,the subject, and ushers the subject into an endless desire chain. To bea subject means to fill in an ontological lack that can never actuallybe fulfilled. Lacan’s revelation of the psychic structure of the selfand its way of function offers us a tool to interpret the cause of thefrustration and disintegration experienced by Conrad’s characters his fictional works.。

基于扎根理论的定性数据主题抽题分析法探析_谢雁鸣

基于扎根理论的定性数据主题抽题分析法探析_谢雁鸣

收稿日期:2008-06-10基金项目:国家十一五科技支撑计划重大疑难疾病中医防治研究项目(2006BA I 04A21)作者简介:谢雁鸣(1959-),女,吉林长春人,研究员,博士生导师,学士,研究方向:中医临床疗效评价方法学。

通讯作者:廖星,E -m a i :l ok fro m 2008@h ot m ai.l co m 。

折的发生,女贞子有雌激素样作用,能抑制骨吸收。

临床结果证实,以补通结合为治法特点的静顺袋泡茶对治疗围绝经期综合征进而预防绝经后骨质疏松症的形成和发展,具有明显的协同作用,我们有必要深入研究其作用机理,以便开发出具有防治结合特点的功能性保健食品。

3 疗效探讨抗衰机理静顺袋泡茶临床应用具有显著疗效,为了探讨其抗衰机理,我们进行了临床性激素测定和动物实验预防绝经后骨质疏松症的研究。

临床研究表明,围绝经期综合征患者经静顺袋泡茶治疗3个月后,反映卵巢功能衰退的雌激素水平有不同程度的上升,治疗组雌激素(E 2)上升的幅度优于对照组(P <0.05),促卵泡激素(FS H )水平治疗后下降,与疗前比较有统计学意义(P <0.05)。

对绝经后骨质疏松模型的动物实验研究提示,静顺袋泡茶干预后能延缓和改善骨质疏松大鼠骨代谢指标(C a ,AKP 、Ca /C r 、P /Cr)的改变(P <0.05~0.01),提示其具有一定的抗骨质疏松作用,机制可能与其抑制骨吸收,减少骨丢失,促进骨形成有关。

在骨质疏松的发病过程中白细胞介素6(I L -6)分泌增多,且又通过刺激破骨细胞活动,促进骨吸收,加速骨疏松的发展,静顺袋泡茶能降低大鼠血清I L -6含量提示其抑制骨吸收作用与减少I L -6分泌有关。

临床研究表明,静顺袋泡茶尚能温和提升雌性激素水平进而对围绝经期综合征早期所出现的烘热出汗与晚期所表现的骨质疏松均具有良好的防治作用。

国内同类技术多以单味药制成袋泡茶,以保健形式提高雌激素水平,作用单一。

高中英语 Unit 9 Wheels Section Ⅲ Lesson 2 Lesson 3教案

高中英语 Unit 9 Wheels Section Ⅲ Lesson 2  Lesson 3教案

Section ⅢLesson 2 & Lesson 3[基础词汇]1.sensitive adj.善解人意的;敏感的2.vocabulary n.词汇;词汇量3.schedule n.时间表;进度表4.content n.容纳的东西;目录5.solar adj.太阳的,太阳光的6.chapter n.章节7.essay n.散文8.shopping n.购物[拓展词汇]9.gentle adj.和善的,温和的→gently adv.温和地10.foolish adj.愚蠢的→fool n.傻子11.responsibility n.责任,负责,职责→responsible adj.负责任的12.impression n.印象,感觉→impress vt.给……留下印象→impressive adj.给人印象深刻的13.reliable adj.可靠的→rely v.依靠14.appreciate vt.感谢;欣赏→appreciation n.感激;欣赏1.pull__up(车辆)停止,停车2.pull__out(火车)驶离车站,出站3.so__far迄今为止4.take__place 举行;发生5.go__through穿过6.check__in登记7.take__part__in参加8.all__the__time一直1.was doing...whe n...正在做……这时……He was__going__through__passport__control__when(正要通过检证处,这时) he realised he didn’t have his passport.2.过去分词短语作后置定语People have been worried about pollution__caused__by__fuels(燃料导致的) like petrol and gas for a long time now.3.the one后的定语从句Well, the one I__like__best(我最喜欢的) crosses the whole of Australia from northwest to southeast.Read the text carefully and choose the best answer according to the text.1.Why are people interested in solar cars?A.Because solar cars use less petrol.B.Because solar cars run faster.C.Because solar cars are clean and safe.D.Because solar cars have stronger power.2.How many races has Marie Logan won?A.Six.B.Two.C.Four.D.Five.3.Why has Marie Logan been writing a book about solar cars?A.Because he wants to take part in car races.B.Because he wants to design more cars.C.Because he wants to make money.D.Because he wants people to have a good impression of solar cars.答案:1-3.CBDSolar① Car RacingSolar racers②have been ing③toAustraliaforyearsfortheWorldSolarCarChallenge.WeinterviewedMarieLoganfromBr isbane.Q:Well, the first question I have for you is simple. What is a solar car?A:Solar cars are cars that use the sun’s energy for power④. That means they do n’t use petrol⑤,gas⑥ or any other fuel, just the sunlight⑦.Q:And why are people interested in solar cars?A:People have been worried about pollution caused by fuels like petrol and gas for a long time now⑧.Solar cars are clean and safe, so there is a lot of interest in them.Q:You’ve been designing solar racing cars for a long time. When did you start? A:Well, I’ve been interested in cars since kindergarten⑨. When I was at university, I started designing my car. I’ve designed five or six different cars so far⑩.And I’ve been taking part in⑪ races for about four years.Q:How many races have you taken part in?A:Let me think. Six.We’ve won two of them!Q:Where do these races take place⑫?A:Well, the one I like best crosses the whole of Australia from northwest⑬ to southeast⑭.It’s about 4,500 km long!①solar['səʊlə] adj.太阳的,太阳光的;solar battery太阳能电池;solar system太阳系②racer['reIsə] n.赛车手③have been ing是现在完成进行时。

学术英语写作-东南大学中国大学mooc课后章节答案期末考试题库2023年

学术英语写作-东南大学中国大学mooc课后章节答案期末考试题库2023年

学术英语写作_东南大学中国大学mooc课后章节答案期末考试题库2023年1.Sequence markersin English are a certain group of items that link sentencestogether into a larger unit of _______.参考答案:discourse2.When the author uses “Methodology” as the title of this section, he/she needsto provide the_______for how the experiment was designed and conducted for the current study.参考答案:rationales3.“Shopping malls are wonderful places.” is a weak thesis statemen t in that itrestates conventional wisdom.参考答案:错误4.One way is to examine one thing thoroughly and then examine the other. Thismethod is called _____ comparison or contrast.参考答案:block5. A strong thesis statement makes a claim that offers some point about thesignificance ofour evidence that requires further argumentation.参考答案:正确6.Strictly speaking, the purpose of _______ is to show similarities while contrastis used to show differences.参考答案:comparison7.In the elements of the Method Section, ______ refer to the precautions taken tomake sure that the data are valid.参考答案:Restrictions8.Paraphrasing is to explain the original ideas of a passage, chapter, article orbook in fewer words.参考答案:错误9.To avoid plagiarism and conform to academic ethics, you need to providereference to every citation and check for plagiarism before submitting your paper.参考答案:正确10.Which of the following tenses could be used to discuss previously publishedworks which is generally considered to be established knowledge?参考答案:The present simple11.Which of the following tenses could be used when the year of publication isstated within the main sentence.参考答案:The past tense12.Which of the following reporting verbs could be categorized as strong?参考答案:reject13.Reporting verbs can indicate either参考答案:All of the options.14.What is included in a complex model of literature review but NOT included ina simple one?参考答案:Research question15.You can choose any information or data from the graphwhen you describeagraph.参考答案:错误16.Redundancy, raising a totally new point, understatement, anticlimax are thetypical issues in structuring the Conclusion.参考答案:错误17.Unlike the Abstract and Introduction,the Conclusions section does providebackground details.参考答案:错误18. 1. The register of the following discourse is____.I, James Bond, take you, JudithKroll, to be my wife, to have and to hold from this day forward, for better, for worse, for richer, for poorer, in sickness and in health, to love and to cherish, till death us do part, according to God's holy law, in the presence of God Imake thisvow.参考答案:static19.What should you do when you write a literature review?参考答案:Include a critical analysis of various opinions from credible sources.20.To end the Discussion section which also has a Conclusion, the author mayadmit what she/he has not been able to do and as a consequence cannotprovide conclusions on.参考答案:正确21.If the authors are to announce the results of their study, they can just statethe results without saying “we think that…”参考答案:正确22.You can use “he or she” to avoid gender discrimination every time when youmean “everyone”.参考答案:错误23.When writing an academic paper, you should nominalize as many words aspossible.参考答案:错误24.Beginning the Discussion section an author would possibly refer back topapers he/she cited in the Review of the Literature.参考答案:正确25.“The U.S. constitution” is not a good title for an essay, because it is toogeneral.参考答案:正确26.“What implications are revealed in my results?” is a question to considerafter drafting the Discussion section.参考答案:错误27.The process paragraphs are usually developed step by step in a chronologicalor logical sequence.参考答案:正确28.The Results Section can only be presented both in diagrams or graphs.参考答案:错误29.The Method Section can be called Materials and Methods in naturalsciences.参考答案:正确30.The Method Section is considered the most important section becauseitappears in the middle of a research paper.参考答案:错误31.Nominalization is the process of converting simple nouns within a sentenceto complex nouns.参考答案:错误32.If you can discuss a cause without having to discuss any other causes thenvery likely it is an indirect cause.参考答案:错误33.Oversimplification should be avoided because many problems have complexcauses and complex effects.参考答案:正确34.First personal pronouns can never be used in academic paper.参考答案:错误35. A weak thesis statement either makes no claim or makes a claim that doesnot need proving.参考答案:正确36.One of the key elements of the Conclusion section is a final judgment on theimportance and significance of the findings in terms of their implications and impact, along with possible applications to other areas.参考答案:正确37.Effects are the consequences of an event and they respond to the question“Why did that event happen?”参考答案:错误。

语言测试方法简介

语言测试方法简介
Integrative tests are best characterised by the use of cloze testing and of dictation.
Oral interviews, translation and essay writing are also included in many integrative tests -a point frequently overlooked by those who take too narrow a view of integrative testing.
2.3
the structuralist
approach (Psychometric-structural Testing)
Character: language learning is chiefly concerned with the systematic acquisition of a set of habits.
Advantage:The integrated skills involvea in tests of dictation include auditory discrimination, the auditory memory span, spelling, the recognition of sound segments, a familiarity with the grammatical and lexical patterning of the language, and overall textual comprehension. Disadvantage:each error in the dictation is usually penalised in exactly the same way.

Anthocyanins Exist in the Circulation Primarily as Metabolites in Adult

Anthocyanins Exist in the Circulation Primarily as Metabolites in Adult

Human Nutrition and MetabolismAnthocyanins Exist in the Circulation Primarily as Metabolites in Adult Men 1Colin D.Kay,*†2Giuseppe (Joe)Mazza,†and Bruce J.Holub**Department of Human Biology and Nutritional Sciences,University of Guelph,ON,Canada and †Agriculture and Agri-Food Canada,Pacific Agri-Food Research Centre,Summerland,BC,CanadaABSTRACT Anthocyanins are reported to have many “health promoting”properties;however,despite numerous reports of their bioactivities,their absorption and metabolism in humans are poorly understood.The objective of this research was to detail the pharmacokinetic parameters of anthocyanins after the administration of a 721-mg oral dose of cyanidin 3-glycosides from chokeberry extract to human subjects.Solid-phase extraction,prepara-tive-HPLC,preparative-TLC,HPLC-diode array detection,HPLC-MS,and NMR were utilized to isolate,identity,and quantify anthocyanins in 0-to 7-h (0,1,2,3,4,5,6,7h)serum and 0-to 24-h urine samples (total individual urine voids over 24h).The cumulative concentration of total anthocyanins (parent and metabolites)detected in the serum (0–7h)was 376.65Ϯ16.20(nmol ⅐h)/L (area under the concentration time curve),reaching a maximum concentration (C max ϭ96.08Ϯ6.04nmol/L)within 2.8h.The parent anthocyanins represented only 32.0%[120.63Ϯ2.85(nmol ⅐h)/L]of the total anthocyanins detected with 68.0%[256.02Ϯ5.23(nmol ⅐h)identified as conjugated metabolites.Additionally,the total urinary excretion of anthocyanins over 24h was 1071.54Ϯ375.46␮g,reaching a maximal rate of excretion (R max ϭ202.74Ϯ85.06␮g/h)at 3.72Ϯ0.83h.Parallel to the serum data,only 32.5%(347.85Ϯ60.61␮g)of the anthocyanins excreted in the urine (total 24h)were the parent compounds with 67.5%(723.69Ϯ92.59␮g)occurring as conjugated metabolites.The metabolites were identified as glucuronidated and methylated derivatives of the parent cyanidin 3-glycosides.The above results indicate that cyanidin 3-glycosides are rapidly absorbed and metabolized extensively following a moderate-to-high oral dose in humans.J.Nutr.135:2582–2588,2005.KEY WORDS:●anthocyanins●cyanidin 3-glycosides●pharmacokinetics●metabolitesWithin the last decade,many studies have focused on the potential biological activities or health effects of anthocyanins in humans (1–3).Although there is a great deal of evidence indicating the bioactivity of anthocyanins,very little progress has been made in establishing the pharmacokinetics of these compounds,with aspects such as absorption and metabolism left essentially unstudied.Previously,it was reported that an-thocyanins were poorly absorbed and circulated in the blood exclusively as unmetabolized parent glycosides (4–6).It is only recently that researchers have begun to suggest that anthocyanins are metabolized;however,the identification of derived metabolites has been limited as a result of their diver-sity and low concentrations in the blood.In our previous investigation focusing on identifying an-thocyanin metabolites in human serum and urine (7),subjects were fed ϳ1.2g of cyanidin 3-glycosides from chokeberries,which resulted in the identification of glucuronide and methyl derivatives.The aim of the present investigation was to de-termine the pharmacokinetics of the cyanidin 3-glycosides in humans as well as to establish the extent of their metabolic fate after a lower,more realistic anthocyanin dose (721mg).Specifically,the chokeberry extract was chosen because itcontained exclusively cyanidin 3-glycosides,thereby permit-ting the monitoring of its metabolites and their pharmacoki-netics.Subsequent investigations will be required to identify the biological activity of these metabolites.SUBJECTS AND METHODSSubjects.Healthy male volunteers (n ϭ3;40Ϯ14.2y old)participated in the cyanidin 3-glycoside consumption intervention.Subjects had a mean BMI of 28.3Ϯ1.6kg/m 2and had no clinical disease as determined using a medical history questionnaire.Subjects were instructed to consume an essentially anthocyanin-free diet (no fruit or vegetables,including foods colored with red or blue dyes)for 2d before the study;they were also asked to avoid taking aspirin or anti-inflammatory medications,and antioxidant or herbal supple-ments for 2wk before the investigation.The major constituents of the anthocyanin-free washout diet were milk,tuna,white bread,chicken,and white pliance with the anthocyanin-free diet was monitored using food diaries and confirmed in baseline samples via reverse-phase HPLC (RP-HPLC)3with diode array detection1Supported by the Heart &Stroke Foundation of Ontario (HSFO)and a Natural Sciences and Engineering Research Council of Canada (NSERC)post-doctoral fellowship (C.D.K.).2To whom correspondence should be addressed.E-mail:cdk14@.3Abbreviations used:C-3-ara,cyanidin 3-arabinoside;C-3-gal,cyanidin 3-galactoside;C-3-glu,cyanidin 3-glucoside;C-3-xyl,cyanidin 3-xyloside;CD 3OD,Methanol-d4;CF 3COOD,trifluoroacetic acid-d;C max ,maximum concen-tration;DAD,diode array detector;E 440/E max ,ratio of the absorbance intensity at 440nm vs.the maximum absorbance intensity;ESI-MS,electrospray ionization MS;m /z,mass to charge ratio;P,peak;PCA,perchloric acid;P-3-gal,peonidin 3-galactoside;Prep-HPLC,preparative HPLC;Rf,reference value;R max ,maxi-mum rate of urinary excretion;RP-HPLC,reverse phase HPLC;Rt,retention time;0022-3166/05$8.00©2005American Society for Nutrition.Manuscript received 31March 2005.Initial review completed 2May 2005.Revision accepted 27August 2005.2582by guest on December 14, 2010 Downloaded from(DAD).The diets met recommended dietary allowances for macro-nutrients,and no energy restrictions were imposed.All subjects gave written informed consent before the commencement of the investi-gation and experimental procedures followed were in accordance with the Helsinki Declaration of 1975as revised in 1983.Study design.Subjects were admitted to the clinic (Okanagan Clinical Laboratory;Penticton,BC)on the morning of the study dates after fasting (12h,24h no alcohol).Baseline urine samples (first void,t ϭ0)were taken in the morning of each study date along with individual urine voids (total volume)over the next 24h (t ϭ0,2,4,6,8,10,12,and 24h).Immediately after baseline (t ϭ0)blood sampling,the volunteers consumed 7.1g of encapsulated (gel caps)chokeberry extract with 250mL of water.The extract contained 4cyanidin 3-glycosides (721.4mg):491.0mg cyanidin 3-galactoside (C-3-gal),175.3mg cyanidin 3-arabinoside (C-3-ara),27.8mg cya-nidin 3-xyloside (C-3-xyl),and 27.3mg cyanidin 3-glucuoside (C-3-glu),as determined by HPLC-DAD (Table 1;Fig.1).Subsequent blood samples were taken at t ϭ1,2,and 3h postconsumption of the extract.The experiment was repeated at a later date (30d wash-out)following the protocol outlined above with one variation.On d 2,blood samples were taken at t ϭ0,3,4,5h and on d 3,at t ϭ0,5,6,7h (n ϭ3;levels ϭ0,1,2,3,4,5,6,7).The sampling regimen was necessary to acquire the volume of blood needed for the analysis.Individual urine samples were also collected over 24h (levels ϭ0,2,4,6,8,10,12,24)at each visit (n ϭ3ϫ3repetitions;y ϭ9replicates).After consumption of the extract,subjects were instructed to consume 250mL of water every hour for 5h with subsequent ad libitum consumption.An anthocyanin-free lunch and dinner was provided for the subjects at 4and 8h postconsumption of the extract.Blood samples (ϳ20mL)were drawn by venipuncture from a brachial vein into 10-mL evacuated glass tubes (2tubes/time point)(Vacutainer;Becton Dickinson).The blood samples were allowed to clot at room temperature for 30min.Samples were then immediately centrifuged (1000ϫg)for 15min at 5°C to recover the serum.Urine samples were acidified with 20␮L of 12mol/L HCl/mL urine uponSPE,solid phase extraction;t ϭ0,baseline;t 1/2,elimination half-life;t 1/2a ,absorption half-life;t max ,time point at which maximal serum concentration oc-curs;t maxR ,time point at which maximal rate of urinary excretion occurs;TFA,trifluoroacetic acid;UV-vis,UV-visible.TABLE 1Identification of anthocyanins and anthocyanin metabolites in human urine and serum after the consumptionof 721mg of cyanidin 3-glycosides 1–3Peak Anthocyanin/identityRtMWparent/daughterfragmentAbsorption spectraTLC (Rf)␭max E 440/E max (as %)1Cyanidin 3-galactoside 23.8449/287280,517310.362Cyanidin glucuronide 27.6463/287280,517310.383Cyanidin 3-arabinoside 29.6419/287280,517310.454Peonidin 3-galactoside33.4463/301280,517310.425Methylated cyanidin glucuronide 36.3477/301280,514370.396Methylated cyanidin glucuronide 37.0477/301280,515320.44Characteristics of anthocyanin standardsCyanidin 3-galactoside 4,523.8449/287280,517310.36Cyanidin 3-arabionside 429.6419/287280,517310.45Cyanidin 3-xyloside 439.0419/287280,517290.48Cyanidin 3-glucoside 4,527.3449/287280,517370.40Cyanidin 543.6287280,526230.80Peonidin 550.8301280,528260.841Identification (retention time and absorption spectra)based on Agilent HPLC-DAD.2Identification (MW)based on Waters microcapillary HPLC-MS.3Comparisons made between serum and urinary samples using HPLC-DAD (Fig.1,C and D )indicated that the serum peaks matched both retention time and UV-vis spectrum with the peaks identified as anthocyanins in the urine,and were therefore regarded as the same compounds.4Data based on analysis of purified anthocyanin from chokeberry extract.5Data based on analysis of purchased standard(Extrasynthese).FIGURE 1Chromatograms of anthocyanins in baseline human urine sample (A ),chokeberry extract (B ),typical serum sample (C ),and typical urine sample (D ).HPLC analysis as outlined in the methods.The identification of each peak represented above (peaks 1–6)is given in Table 1.Urine and serum pharmacokinetic data of each compound represented by peaks 1–6are given in Tables 2and 3,respectively.PHARMACOKINETICS OF CYANIDIN 3-GLYCOSIDES 2583by guest on December 14, 2010Downloaded fromcollection.The serum and urine were stored at Ϫ80°C after removal/collection.Materials/reagents.The chokeberry extract (no.74190,lot L18010)was purchased from Artemis International.The anthocya-nin standards,cyanidin 3-glucoside chloride,cyanidin 3-galactoside chloride (ideain chloride),peonidin 3-glucoside chloride,cyanidin chloride,and peonidin chloride were purchased from Extrasynthese.Formic acid (Fisher Scientific),hydrochloric acid (HCl),trifluoro-acetic acid (TFA),and glacial acetic acid (DH)were all reagent grade;all solvents used for HPLC analysis were HPLC grade.Anthocyanin analysis.The chokeberry extract,serum,and urine anthocyanins were quantified via HPLC-DAD.Individual serum sam-ples were collected every hour for 7h (n ϭ3)and individual urine voids were collected (separately)over a 24-h period (n ϭ3ϫ3repetitions)as detailed in the study design.Every serum and urine sample was analyzed and quantified individually via HPLC-DAD before pooling for identification purposes.After quantification,urine samples were pooled for purification (XAD adsorption chromatogra-phy),isolation [preparative (prep)-HPLC and prep-TLC],and iden-tification (HPLC-MS;HPLC-DAD;TLC;NMR).Identification of the anthocyanins was based on the matching of molecular weight (parent and daughter fragments),retention time (Rt),␭maxvis ,E 440/E max ,and reference (Rf)values with those of available anthocyanin standards as well as isolated chokeberry anthocyanins (Table 1).TLC data (post-acid hydrolysis)were utilized for additional confirmation when considered necessary.Peaks lacking absorption maxima in the 280and 520nm range were not considered anthocyanin metabolites,and no attempt was made to identify these unknown compounds.Selected pharmacokinetic variables were determined for the identi-fied compounds from the initial HPLC quantitative results (Table 2and 3;Figs.2and 3).Methods of extraction [C 18solid-phase extraction (SPE)]were modified from Kay et al.(7)and Tsuda et al.(8).The anthocyanins were extracted from biological fluids before HPLC analysis using disposable SPE C 18cartridges (Supelclean ENVI-186mL 2000mg;Sigma;lot #SP2419C).Unfiltered blood serum (4mL)or 2–10mL of unfiltered acidified urine (2mL,t ϭ0–9h;or 10mL,t ϭ10–24h)were utilized for the extraction.Individual blood and urine samples (nonpooled)were extracted in duplicate,and each extract was in-jected into the HPLC column in duplicate (total of 4injections/sample)for quantitative HPLC analysis.Purification of anthocyanins in pooled human urine samples (postquantitative HPLC analysis)was performed using Amberlite XAD-7polymeric adsorbent (Sigma;Lot #77H0157)before isolation of individual anthocyanin/metabolite peaks using prep-HPLC.The procedure was based on general methods as described by Markham (9).The column (50ϫ3.0cm)was filled with presoaked (24h EtOH:H 2O v:v)XAD-7resin (volume of 212cm 3)and loaded with 1L of unfiltered acidified urine (pH Ϸ2.5).The column was drained under gravity,then washed with 500mL acidified H 2O (0.1%TFA),followed by 500mL MeOH:H 2O (30:70;0.1%TFA;flow rate 10mL/min).Finally,the anthocyanin extract was eluted with 500mL of MeOH:H 2O (75:25;0.1%TFA;flow rate 10mL/min)and evapo-rated.The anthocyanin-rich urine extract was then further purified via prep-HPLC.Analytical HPLC analysis was performed on an Agilent 1100series HPLC (Agilent Technologies)using a Zorbax SB C 18RP column (5␮m,4.6ϫ250mm)with a Supelguard LC-18guardTABLE 3Pharmacokinetic parameters of cyanidin 3-glycosides and corresponding metabolites in human serumafter the consumption of 721mg of cyanidin 3-glycosides 1PeakAnthocyanin/identityC max T max 2AUC t 1/2a 3nmol/Lh (nmol ⅐h)/L h 1Cyanidin 3-galactoside 23.36Ϯ 2.33 2.5(2–3)66.64Ϯ 3.27Ͻ1.352Cyanidin glucuronide 14.51Ϯ 4.04 2.0(2)90.93Ϯ 4.083Cyanidin 3-arabinoside 8.85Ϯ0.50 3.5(3–4)53.99Ϯ 2.43Ͻ1.674Peonidin 3-galactoside3.76Ϯ0.784.0(4)30.67Ϯ 1.125Methylated cyanidin glucuronide 12.81Ϯ0.42 2.5(2–3)34.42Ϯ 2.776Methylated cyanidin glucuronide 32.79Ϯ10.882.5(2–3)100.00Ϯ12.851Pharmacokinetic data are means ϮSD,n ϭ3,over 7h as quantified via HPLC-DAD (Table 1).2Values are medians (range).3t 1/2a :precise absorption half-lives for 2of the 3subjects could not be determined because there was only one serum sample (t ϭ1h)during the absorption phase.Therefore,as a result of the rapid absorption phase (Ͻ1h)for 2of the 3subjects,SDs cannot be specified.The absorption half-life values above are from 1subject only and it can be assumed that the mean absorption half-life for the 3subjects would be Ͻ1h.For a more accurate estimate of absorption additional half-life samples are required before the end of h 1.The t 1/2a value was determined using the method of residuals (14).TABLE 2Pharmacokinetic parameters of cyanidin 3-glycosides and corresponding metabolites in human urineafter the consumption of 721mg of cyanidin 3-glycosides 1PeakAnthocyanin/identityQuantityR maxt maxR t 1/2␮g␮g/h h h 1Cyanidin 3-galactoside 267.55Ϯ90.8248.74Ϯ14.80 3.39Ϯ1.14 3.72Ϯ0.422Cyanidin glucuronide 173.25Ϯ86.4639.47Ϯ20.77 3.17Ϯ0.86 3.63Ϯ0.563Cyanidin 3-arabinoside 80.30Ϯ31.3013.17Ϯ 4.68 2.94Ϯ1.39 4.05Ϯ0.824Peonidin 3-galactoside103.88Ϯ35.6319.27Ϯ7.74 3.39Ϯ1.14 4.28Ϯ0.595Methylated cyanidin glucuronide 111.06Ϯ60.6119.61Ϯ10.51 4.05Ϯ1.21 4.53Ϯ0.926Methylated cyanidin glucuronide 335.50Ϯ187.6666.63Ϯ37.134.05Ϯ1.214.39Ϯ0.831Pharmacokinetic data are means ϮSD,n ϭ9,over 24h as quantified via HPLC-DAD (Table 1).KAY ET AL.2584 by guest on December 14, 2010Downloaded fromcolumn (C 185␮m,4.6ϫ20mm;Supelco,Sigma-Aldrich).The following procedure was modified from previously published methods (7,10).Prep-HPLC separation of individual anthocyanins from pooled urine samples was performed on a Waters Chromatographic system (Waters)comprised of 3Model 510pumps,and a Model 490pro-grammable multiwavelength detector set at 525nm.The preparative column system (Waters PrepPak)consisted of 2Nova-Pak HR C 18radial compression cartridges (25ϫ100mm;6␮m,60Å;PrepPak Cartridge;Waters)with a Nova-Pak HR C 18guard insert (Waters).Injections were carried out on a manual injection port (Rheodyne)equipped with a 500-␮L injection loop.The column and injector were kept at ambient temperature,with an injection volume of 250–500␮L.The mobile phase consisted of 0.1%TFA in water (solvent A)and 100%MeOH (solvent B).The flow rate was 15mL/min with an isocratic run of 80%A and 20%B.Peaks on the chromatogram corresponding to anthocyanins,as identified by spec-tral analysis (peaks detected at 525nm with ␭max 250–300and 500–550nm),were collected manually from the prep-HPLC column and concentrated using a rotary evaporator.The remaining H 2O was removed in a freeze-dryer and samples were sealed under nitrogen gas and stored at Ϫ80°C until further analysis.TLC procedures were based on methods described by Wagner and Bladt (11).Normal phase prep-TLC was utilized for the final purifi-cation of individual anthocyanins separated from the pooled urine samples (20ϫ20250-␮m silica gel Redi/plates;Analtech).The solvent system consisted of ethyl acetate,glacial acetic acid,formic acid,and H 2O (100:11:11:26).After the plates were developed,the anthocyanin bands were removed and dissolved in 5mL of MeOH containing 0.1%formic acid.The final solution was filtered through a 0.45Ϫ␮m polyvinylidene fluoride syringe filter,evaporated in arotary evaporator,and brought to dryness in a freeze-dryer.The remaining extract was sealed under nitrogen gas and stored at Ϫ80°C until further analysis.For the postacid hydrolysis of anthocyanins for verification of aglycones,0.20-mm silica gel 60analytical TLC plates (Macherey-Nagel;Batch 901/021)containing a fluorescent indicator (UV 254)were used.Acid hydrolysis of the anthocyanin glycosides was achieved by dissolving a portion of the dry anthocyanin extracts in 200␮L of 2mol/L HCl.The solution was then sealed under nitrogen gas and heated to 100°C for 1.5h.The samples were then cooled immediately in an ice bath and plated using the above solvent system.MS identification of individual compounds was conducted postseparation via prep-HPLC and prep-TLC (as outlined above).The analysis was carried out on a Waters Alliance 2695HPLC coupled serially with a Waters 2996photodiode array detector and a Waters ZQ 2000quadrupole analyzer utilizing the electrospray ionization interface (ESI-MS)(Waters).The chromatographic separation was performed on a 250ϫ 2.0mm Synergi 4-␮m Max-RP 80Åcolumn (Phenomenex)with a 4ϫ2mm Phenome-nex Max RP guard cartridge (Phenomenex).Injection volumes were 2␮L.The mobile phase consisted of an acidified (0.18%v:v acetic acid)water:acetonitrile mixture (95:5)(solvent A)and 100%acetonitrile (solvent B).The flow rate was 130␮L/min;the solvent gradient program used 100%A at 0–2min and was ramped to 100%B at 60min.The instrument was operated in electrospray positive ion mode (ES ϩ).Micromass ZQ single quadrupole MS with electrospray interface and MassLynx 4.0software (Micro-mass)was used for data acquisition.The MS parameters were loosely based on methods previously published by Felgines et al.(12)and Garcı´a-Beneytez et al.(13).NMR spectra were obtained on a Bruker Avance DRX 500MHz spectrometer (Bruker Biospin),equipped with a cryoprobe,at 300K.For 1H (500MHz)NMR,a solvent mixture of methanol-d4to trifluoroacetic acid-d (CD 3OD:CF 3COOD)(98:2,v:v,200␮L)was used and ␦values were referenced to CD 3OD (CHD 2OD at 3.30ppm).Analysis of the 1H NMR spectra was based on the comparison of the chemical shift and relative intensity of the signals with those of standard compounds.Statistical analysis.The primary analyses were performed on urinary values (urinary values only)using the mixed models proce-dure (PROC MIXED)in SAS (version 9.1;SAS Institute).The data are presented as means ϮSD unless otherwise stated.The distribu-tions of anthocyanins were corrected with natural logtransforma-FIGURE 2Time course of total,parent,and metabolized antho-cyanins in human urine (A )and serum (B )of subjects after the con-sumption of 721mg of cyanidin 3-glycosides.For urinary (A ;n ϭ9replicates)and serum (B ;n ϭ3)data,values are means ϮSD as represented by vertical bars.(A )Different letters for the points indicate that the concentrations of total urinary anthocyanins differed across time,P Ͻ0.05.Identification of each peak as labeled in the legend (as peaks 1–6)is given in Table 1.Urinary and serum pharmacokinetic data of each compound represented by peaks 1–6,is given in Tables 2and 3,respectively.FIGURE 3Cumulative time course of individual anthocyanins excreted in human urine after the consumption of 721mg of cyanidin 3-glycosides.Values are means ϮSD (n ϭ9replicates)as represented by vertical bars.Different letters for the lines indicate that the concen-trations differed at the level of the individual anthocyanin,P Ͻ0.05.For peak identities,refer to Table 1.For urinary and serum pharmacokinetic data of each compound represented by peaks 1–6,refer to Tables 2and 3,respectively.PHARMACOKINETICS OF CYANIDIN 3-GLYCOSIDES 2585by guest on December 14, 2010Downloaded fromtions,and unadjusted values are reported.The total level of antho-cyanins as well as the levels of individual anthocyanin species were evaluated over time.The model included time (levels ϭ0,2,4,6,8,10,12,and 24h)and anthocyanin species (levels ϭ1,2,3,4,5,6)as fixed effects,and subjects (n ϭ3)and their replicate treatments (3replicates)were treated as random variables (y ϭ9replicates).For all analyses,the significant main effects (P Յ0.05)were investigated using the Tukey-Kramer test.In addition,noncompartmental pharmacokinetic evaluation of urine and serum parent compounds,and their metabolites was per-formed on untransformed data according to standard methods (14).For urinary variables,there was no effect of subject (P ϭ0.3);therefore,subject (n ϭ3)and their replicate treatments (3replicates)were collapsed (y ϭ9replicates)for the determination of means (ϮSD)in the pharmacokinetic analysis.The analyses of serum variables included 3subjects with no replications and are presented as means ϮSD (n ϭ3;no replicates).Calculation of the area under the plasma concentration time curve was based on the mean serum concentra-tions of individual subjects using the trapezoidal rule.Absorption half-lives were determined graphically (SPSS SigmaPlot,IL)using the method of residuals.The geometric means of the elimination half-lives were determined graphically from the renal excretion rates of individual subjects.RESULTSSerum and urine samples collected before the administra-tion of the chokeberry extract (baseline,t ϭ0)contained no detectable anthocyanins (Fig.1A ).Postconsumption,both cyanidin 3-galactoside and cyanidin 3-arabinoside [peak (P)1,3;Fig.1]were present in the serum and urine.Both glucuronidated (loss m /z ϭ176upon fragmentation)and methylated (m ϩ14)derivatives of cyanidin were also present.In total,4derivatives/metabolites were isolated from the urine in sufficient quantities for structural identification.One metabolite (P2;Fig.1C and D )was identified as a cyanidin glucuronide,as indicated by its molecular ion at m /z 463and fragment at m /z 287,indicating a loss of m /z 176upon fragmentation (representing a glucuronide residue;m /z ϭ176;Table 1).Additionally,the hydrolysis of this compound re-sulted in an aglycone with the same HPLC Rt,UV-vis spectral data,and TLC Rf value as the purchased cyanidin standard.There was insufficient evidence to determine the exact posi-tion of the glucuronide residue because the NMR spectra were uninterruptible (result of insufficient quantity and poor solu-bility).The second identified metabolite (P4;Fig.1C and D )had the chemical characteristics of a methylated derivative of C-3-gal,having a parent ion of m /z 463,daughter fragment of m /z 301(consistent with methylation;m /z 287ϩ14ϭ301),and loss of m /z 162upon fragmentation (indicative of a hexose sugar;m /z ϭ162;Table 1).The hydrolysis of the compound resulted in an aglycone with similar HPLC Rt,UV-vis spectral data,and TLC Rf value as the purchased peonidin standard.There was insufficient evidence to determine the exact posi-tion of the methylation and glucuronidation because the NMR spectra were uninterruptible (result of insufficient quantity and poor solubility).Two other metabolites (P5,6;Fig.1,C and D)were identified as methylated derivatives of cyanidin glucuro-nide,having parent ions of m /z 477and daughter fragments of m /z 301,indicating a loss of m /z 176upon fragmentation (consistent with glucuronic acid residue;m /z ϭ176;Table 1).The derivatives were dissimilar to peonidin,having different HPLC and TLC parisons made between serum and urinary samples using HPLC-DAD (Fig.1,C and D;Table 1)indicated that the serum peaks matched both reten-tion time and UV-vis spectrum of the peaks identified as anthocyanins in the urine,and were therefore regarded as the same compounds.As a result of the sampling regimen,the mixed models procedure was performed on urinary variables only.There was a significant main effect of time (P Ͻ0.0001)for the total (P1–6)and individual level of anthocyanins (P1,2,3,4,5,6),as well as a significant interaction between time and anthocyanin species (P ϭ0.0016).Additionally,the level of total antho-cyanins did not differ among the 3subjects (P ϭ0.30).Serum variables were utilized only for pharmacokinetic analyses.The pharmacokinetic analysis of urine and serum variables (Tables 2and 3)and their graphical representations (Figures 2and 3)utilized untransformed data as was previously described (14).The results of both urinary (Table 2)and serum (Table 3)analyses indicated that parent compounds and their metabo-lites had similar pharmacokinetic profiles (T max and T 1/2).DISCUSSIONIn previous investigations,it was questioned whether the concentration of anthocyanins observed in the blood was sufficient to yield biological activity.We hypothesized that unidentified anthocyanin metabolites may contribute to the reported effects of anthocyanins.A previous investigation by our group identified anthocyanin metabolites in human serum and urine after the consumption of cyanidin 3-glycosides in chokeberries (7).Subjects were fed ϳ1.2g of cyanidin 3-gly-cosides,leading to the identification of glucuronide and meth-ylated derivatives in the serum and urine.The aim of the present investigation was to identify metabolites and their time course (pharmacokinetics)after a lower,more realistic anthocyanin dose.The present investigation involved a 721-mg oral dose of cyanidin 3-glycosides with the subsequent collection of serum over 7h and urine over 24h.This dose is equivalent to ϳ120–230g of whole berries (fresh weight)(15).Additionally,a 721-mg dose is roughly the median dose of 12reviewed anthocyanin human consumption trials in the literature (788Ϯ883mg)(5,10,12,16–24).The chokeberry extract as utilized in the present investigation was chosen for its simplistic anthocyanin profile,consisting of only cyanidin 3-glycosides.The use of a fruit extract containing only one anthocyanidin species (cyanidin)was crucial for establishing the origin of methylated cyanidin derivatives.Further inves-tigations are required to identify the biological activity of these metabolites.In the present investigation,no anthocyanins were identi-fied in the serum or urine of fasting subjects suggesting that the washout phase and prestudy dietary exclusion of anthocyanins was sufficient.Glucuronidation was the major metabolic path-way observed for anthocyanin metabolism in the present in-vestigation,representing 59.8and 57.8%of the total antho-cyanins detected in the blood and urine,respectively.Methylation was the second most commonly observed meta-bolic transformation for anthocyanins,representing 43.8and 51.4%of the total anthocyanins detected in the serum and urine,respectively.Even though only a few researchers have reported glucuronidated and methylated anthocyanins in the urine and blood of humans and animals (7,8,12),methylated and glucuronidated derivatives of the flavonoid quercetin are well documented (18,25,26).Although some recent investi-gations described the detection of anthocyanin metabolites in urine,this is the first study to give detailed pharmacokinetic parameters for anthocyanin metabolites.In the present investigation,no attempt was made to iden-tify HPLC peaks lacking characteristic anthocyanin profiles (maxima in the 280and 520nm range).There are likely other anthocyanin metabolites (breakdown products)present in the serum and urine with absorbance outside the 240-to 525-nmKAY ET AL.2586 by guest on December 14, 2010Downloaded from。

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norms but often from complex attributionaland perceptual processesthat the individual being influenced engages in to understandthe causes of the behaviors and opinions of the influencingagents or referents(see Folkes 1988; Mizerski,Golden, and Kernan 1979). In Calderand Burnkrant literature, the consumer-behavior (1977) first proposed an attributional model of coninfluences.This model, while never sumerinterpersonal tested in its entirety, proposes that conformity in the purchase of products and services is mediated by the consumer's attributionsregardingthe likely reactions of referentothersto his/her productchoices. Similarly, conformityto peersis often consideredto be one of the hallmarks of adolescent and young adult behavior (Brown,Clasen,and Eicher 1986). However,it is somewhat surprisingthat little effort has been made to ascertain the nature of adolescents' cognitive responses to peer pressures.We believe that a cognitive perspective, focusing primarilyon encouragingyoung people to seek explanations(or certain types of explanations) for a peer group's illicit behavior, offers considerable promise as a means of reducingconformityin the consumption of drugs and alcohol (see Ross, Bierbrauer, and Hoffman 1976). Therefore, we investigated cognitive responses to conformitypressurein the domain of drugand alcohol abuse among young people, particularlythe attribuC 1992by JOURNALOF CONSUMER RESEARCH, * Vol. 19 * June 1992 Inc. All rightsreserved. 0093-5301/93/1901-0001$2.00
University of South Carolina
Scholar Commons
Faculty Publications Marketing Department
6-1-1992
An Attributional Analysis of Resistance to Group Pressure Regarding Illicit Drug and Alcohol Consumption
Randall L. Rose
University of South Carolina - Coluniversity of South Carolina - Columbia, bbearden@
An
to
Attributional Analysis
Group Alcohol Pressure Consumption
of
Resistance Illicit Drug
regarding
and
RANDALLL. ROSE 0. WILLIAM BEARDEN JESSE E. TEEL*
This article investigates the role of attributionalthinking in generating resistance to pressures toward conformity in the illicitconsumption of drugs and alcohol. The results of four studies regarding how conformity influences illicitdrug and alcohol consumption among high school and college students are reported. Instudy 1 more than two-thirds of the respondents reported concern for the implications of their own dissent or compliance regarding the reactions of their peers. Study 2 demonstrated a significant relationship between high school students' attributionalthinking concerning a peer group's illicit beer consumption and conformity, expressed as intentions to drinkthe beer. In study 3, in-depth interviews with high school students provided insight into the realism of the conformity scenarios used in the research and the types of conformity pressures experienced by young people. In study 4, dimension, and several specific attributions locus of causality, an abstract attributional were shown to be significantly associated with conformity in the consumption of marijuana.
*RandallL. Rose is assistantprofessorof marketing,William 0. Beardenis professorof marketing,and Jesse E. Teel is professorof University marketing, in the Collegeof BusinessAdministration, all of South Carolina,Columbia,SC 29208. The authorswould like to thank Bob Burnkrant, Joel Cohen, Valerie Folkes, Peter Reingen, TerryShimp,and the three reviewersfor their insightsand encouragement.
2
JOURNAL OF CONSUMER RESEARCH
tional natureof their thinking and the implications of attributionalthinking for conformity. As background to a discussionof the role of attributionalprocessingin conformity,we present a brief review of the literature concerningconformityand substanceabuse.
ccordingto a recentGallop poll, drugand alcohol abuse has become the issue of greatestconcern to many Americans.The influence of other persons, individuallyand in groups,is frequentlyused to describe the decision to use illicit drugs and alcohol, especially among adolescents and young adults (e.g., Halebsky 1987;Marcos,Bahr,and Johnson 1986;Winfree 1985). President George Bush, in his "National Strategyon Drug Abuse," a nationally televised address on September 5, 1989, blamed peer pressurefor much of the nation's drug problem and argued that peer pressure could also affect a reduction in drug abuse. Unfortunately, little researchregardingthe consumption of either traditional products and services or illicit drugs and alcohol has been directed toward understanding the mediating processes through which pressures to conform are ultimately manifestedin conforming behaviors. influence from a cognitive perspective Interpersonal stems not so much from a passive submissionto group
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