L_H13_Okamoto[1]
美泰里氏硬度计 MH310 使用说明书
美泰里氏硬度计MH310使用说明书南北潮商城目录1概述 (3)1.1产品特点 (3)1.2主要用途及适用范围 (3)1.3技术特性 (3)1.4仪器配置 (4)1.5工作条件 (5)1.6注意事项 (5)2结构特征与工作原理 (5)2.1结构特征 (5)2.2主显示界面 (6)2.3键盘定义 (6)2.4工作原理 (7)3测量前的准备 (7)3.1仪器准备 (7)3.2冲击装置选择 (8)3.3被测工件的准备 (8)4测量方法 (8)4.1启动 (8)4.2加载 (8)4.3定位 (9)4.4测量 (9)4.5读取测量值 (9)4.6特别提示 (9)5仪器操作 (10)5.1仪器开、关机 (10)5.2材料设置 (10)5.3硬度测试/强度测试设置 (10)5.4冲击方向设置 (11)5.5平均次数设置 (11)5.6存储功能 (11)5.7打印数据 (12)5.8时间日期设置 (12)5.9恢复出厂设置 (12)5.10背光功能 (13)5.11自动关机 (13)5.12打印纸卷安装 (13)5.13电池充电 (13)5.14电池更换 (13)5.15与PC机连接 (13)5.16提示信息对照表 (14)6保养和维修 (14)6.1冲击装置维护 (14)6.2仪器使用注意事项 (14)6.3故障分析与排除 (14)6.4仪器维修 (14)附录 (15)附表1 (15)附表2 (16)附表3 (16)附表4 (17)用户须知 (18)1.1产品特点●依据里氏硬度测量原理，可以对多种金属材料进行检测；●一台主机可配备6种不同冲击装置使用，自动识别冲击装置类型，更换时无需重新校准；●支持“锻钢（Steel）”材料，当用D/DC型冲击装置测试“锻钢”试样时，可直接读取HB值，无需人工查表；●采用高对比度的段码液晶显示，操作简单、方便；●具有示值校准功能；●可存储最大100组（冲击次数32～1）硬度测量数据，每组数据包括单次测量值、平均值、冲击方向、次数、材料、硬度制等信息；●显示屏上有剩余电量指示图标，可实时显示电池剩余电量。
MCNP 6.1.1 - Beta Release Notes
LA-UR-Approved for public release;distribution is unlimited.Los Alamos National Laboratory, an affirmative action/equal opportunity employer, is operated by the Los Alamos National Security, LLC for the National Nuclear Security Administration of the U.S. Department of Energy under contract DE-AC52-06NA25396. By acceptance of this article, the publisher recognizes that the U.S. Government retains a nonexclusive, royalty-free license to publish or reproduce the published form of this contribution, or to allow others to do so, for U.S. Government purposes. Los Alamos National Laboratory requests that the publisher identify this article as work performed under the auspices of the U.S. Department of Energy. Los Alamos National Laboratory strongly supports academic freedom and a researcher’s right to publish; as an institution, however, the Laboratory does not Title: Author(s): Intended for: 14-24680MCNP 6.1.1-Beta Release NotesTim GoorleyRelease with MCNP 6.1.1MCNP TM 6.1.1 - Beta Release NotesTim Goorley6/24/2014This document provides an overview of the MCNP 6.1.1 beta release, including the new features, bug fixes and other important changes to how the code runs. The production version of the code, MCNP 6.1, was released in June 2013.I.IntroductionII.New Features – CodeIII.New Features - DataIV.Code FixesV.Changes to Nuclear and Atomic Dataments on using the Beta release for Criticality Safety applicationsVII.Known IssuesVIII.Work in ProgressIX.Changes in Running the codeX.Provided Executable Compilation DetailsXI.Installation InstructionsI.IntroductionThe latest release of MCNP6 Monte Carlo code is designated MCNP 6.1.1 beta. We recommend that this beta release is not to be used for production level calculations, because it does not have the same level of verification, validation and testing as MCNP 6.1, both in the evaluation of new features and existing feature interoperability, and testing on a wide variety of hardware andoperating systems. However, if users wish to perform their own V&V, it is ultimately up to their careful consideration how these executables should be used. This release does have the samehigh level of confidence for the existing capabilities released in MCNP 6.1.We have created this release for users interested in testing the new capabilities that are mostly for homeland security and non-proliferation applications. The beta version does not include newcapabilities for the criticality safety community, but does run significantly faster than theproduction version. The MCNP package available from RSICC contains the final productionversion of MCNP 5 and MCNP X, and the latest production version of MCNP 6, in addition to this beta release and the associated nuclear and atomic data. Additional information on the new features can be found in the associated MCNP references collection, and in several cases, in the revised MCNP 6 Manual.II.New Features - Codea.Added Correlated Gamma Multiplicity (CGM) model from LANL, to produce correlatedsecondary particles (gammas) for ACE-based neutron interactions. This feature also providesmore realistic neutron and gamma multiplicities. CGM is invoked by setting the 9th entry on thephys:n card to 2 (effectively a switch between no/ACE/CGM). The default is still to use ACE data. A test suite (Testing/Features/CGM) was added to test this capability. Artf28690b.Enabled delayed alphas from nuclear interactions as well as spontaneous delayed-alpha sources(PAR=sa and PAR=zaid on SDEF card). Added delayed-alpha emission spectra to thedelay_library_v2.dat file (and higher versions). artf29914c.Allow spontaneous neutron (PAR=sn)_and beta (PAR=sb) sources. Enable the production ofthese with heavy-ion decay as well (PAR=ZZAAA ERG=0). Also added DBCN(10), for user input of the half-life threshold for stability (default 1.5768e16 s) Artf27040, artf27488d.Improved time integration of secondary-particle production from spontaneous decay. Althoughtime-dependence is not used for the sampling of source particles from spontaneous decay, the time-dependent differentials are still integrated to obtain total production values. The linear algorithm used to integrate these complex distributions, that are often spread over many decades in time (e.g., 1e-6 to 1e20 s), can lead to significant errors in the integral values (exceeding 50% in some cases). This improvement reset the decay constants to unity and redistributed the 234 time steps to within 20 s (~10 decays), just for the case of spontaneous decay. User control is provided to allow the user to truncate a decay chain after a certain number of decays (~1s per decay). This integration improvement should keep integration errors to <10%. Artf30049e.Added correlated sampling of Delayed-Particle Production. This feature offers correlatedsampling of delayed particles from spontaneous and induced decay. When chosen, this treatment invokes a Monte Carlo sampling of Cinder90 decay branches. Delayed particle spectra from only the sampled branch are subsequently sampled. Use the SAMPLING=[all|correlate] keyword on the ACT card.Three bug fixes involving CINDER_MOD.F90 were also included. Artf28681f.Several unstructured mesh improvements have been made. Added the capability to use theunstructured mesh geometry with electrons, positrons, and protons (or any heavy chargedparticle) transport. Previously only neutron and gamma transport were supported. Pulse height tallies, DXTRAN and point detectors now work with the UM. Overlapping models can be selected globally or by part. Background materials are no longer treated as a void, it is thematerial specified by the container cell. Because of a tracking algorithm change, neutron/photon transport problems have speedups of 20-50%. See the updated UM users’ Guide. The code writes version 4 of the eeout file. The um_post_op utility now has the ability to write a single edit to a file ordered by position. The um_pre_op utility can now check element volumes, and check for twisted and deformed elements in the Abaqus.inp file. Artf30091g.The number of digits in the mctal and output file were increased for large integers. Artf30637h.Several performance enhancements were implemented. Lines of fortran code were revised toreduce setup and wall-clock runtimes. Includes changes to variable initialization, and binary searches. These improvements are always enabled and there are no user controls for them.Artf30039, artf23878i.Added the capability to create Cerenkov optical photons from charged particles and havereflection/refraction at surfaces. The keywords refi, refc, refs added to material cards, 16th entry on phys:<pl> card to control/bias Cerenkov production. Default is off. Artf27508j.Added the Compton Image Tally option, which creates a special tally option (FT card) that enables the creation of two lattice detector grids which can be used in coincidence to create backward-projected Compton circles via a straightforward imaging algorithm. The overlapping of the Compton circles across particle histories should form an image of the source. Artf28594.k.The Cosmic source feature (PAR=cr) now includes heavy ions. A test suite(Testing/Features/COSMIC) was added to test this capability. Artf29193.III.New Features – Dataa.The 3rd version of Background.dat file was released and is located in the MCNP_DATAdirectory. It includes cosmic-source calculations on a 5x10 (latxlong) grid on Earth, and NORM calculations on this grid for the US. This updated file is now the default. Artf28699 andArtf31113b.The file kcksyst.dat was added to the MCNP_DATA directory. It contains parametersystematics for level density calculations for a wide range of nuclei. It is needed by CoH and/or CGM for poorly known nuclei. The reference for how those systematics were established is:"Phenomenological Nuclear Level Densities using the KTUY05 Nuclear Mass Formula forApplications Off-Stability," T.Kawano, S.Chiba, H.Koura. J. Nucl. Sci. Tech. 43(1) 1-8 (2006)c.The file ripl-3.dat was added to the MCNP_DATA directory. It is a compilation of all knowndiscrete level data (so low-lying levels) for each nucleus, as evaluated in the RIPL-3 IAEAcollaboration effort (https:///RIPL-3/)d.Update to the delayed particle emissions library, delay_library.dat, which now includes alphaemissions. The new name is delay_library_v3.dat. MCNP 6.1.1beta will now automatically look for a file named delay_library_v3.dat. artf29914, cmmt124209.IV.Code Fixes: The following is a list of the titles of higher priority bugs that have been fixed since the production release.artf32748 : Fix for cell-source rejection in unstructured embedded geometriesartf32340 : MCNP6 - fix error messages in LLNL fission packageartf32339 : MCNP6 – bug fix for translation of torus surfaceartf31799 : DF dose tally IC=99 option has bug in neutron responseartf31254 : LNK3DNT tracking accuracy bugartf30048 : Unrealistic scores in segmented electron energy deposition tally.artf26783 : Time structure of delayed particles contains anomalous structureartf30637 : Plot and mctal large float and integerartf31704 : Some problems show xsdir lines printed to screen during initializationartf31044 : NCIA options not working correctlyartf30139 : Bad Trouble error in acecasartf31027 : Table 100 print errorartf31263 : FT ROC Tally Option Fails to Rendezvous Properlyartf27841 : WWG Bugartf31264 : um_post_op misc. fixesartf30700 : Incorrect creations time of secondary particles from electrons in magnetic field cells artf31200 : Energy deposition embee gives Nan"s for void cellsartf31180 : DXTRAN diagnostic prints using DD card limitsartf27105 : Magnetic field tracking loses particles if the field direction is perpendicular to a plane surfaceartf29785 : Invalid particle termination before tallydartf27097 : FT TMC option gives bad resultsartf30140 : fixcom bug fixartf29966 : Fission Multiplicity Crashartf21940 : DXTRAN diagnotic table incorrect for kcode problemsartf18109 : PWT card and large source weights can produce wrongartf27742 : Burnup Compatability with Other KCODE Featuresartf25705 : Continuous S(alpha,beta) sampling bugartf28915 : Tally Plots Show Zero Ordinates for Nonzero Tallyartf29432 : Cyclic Time bins created incorrectly from keywordsartf29431 : Cosmic Source Zero Weight Particlesartf29415 : Cosmic Source Option Should Use Provided Polar and Azimuthal Anglesartf29404 : Segfault in expirx print in sourcebartf29305 : Enable FT ROC with FT PHLartf29292 : Tracking Differences with MPI and Delayed Particlesartf29205 : Event Log Analyzer bugs & minor fixesartf18748 : Integer overflow in dxtran diagnotics outputartf29075 : Use of DF 99 Dose option with TMESH tallies is improperartf28704 : WW MESH keyword "kmesh" doesn not accept degrees or radiansartf28857 : Density-Effect Bug with Mixturesartf28953 : Conductor Specification Bugartf28819 : MCTAL Plots For Flux Image Are Often Not Correctartf28122 : New eprdata does not transport photons at the upper energy limit (100 Geartf28646 : particle weight drops without weight cutoff being played when using SI Aartf28599 : Cross Section Plotter Fails to Ignore Coherent Scattering in Some Casesartf28387 : Setting tags for light ion recoil and aceionartf31834 : Electron transport hangs in French UM Problemartf31130 : UM output for regression testingartf31043 : F8 tallies yield incorrect results with unstructured meshV.Changes to Nuclear and Atomic DataThe original S(alpha,beta) data tables for uranium in uranium oxide (u-o2 and u/o2), zirconium in zirconium hydride (zr-h and zr/h), and silicon dioxide (sio2) had some problems in the header of those files. Updated and corrected versions of those files have been provided with a ZAIDextension of 3xt; 2xt is the extension of the originally released files. The new (corrected) datatables are for: u-o2.30t ..... u-o2.37t, zr-h.30t ..... zr-h.37t, sio2.30t ..... sio2.36t. These new tables are the default. The following xsdir files have been revised to include these updates:xsdir_mcnp6.1, xsdir_mcnp6.1_endfb-7.0, xsdir_mcnp6.1_endfb-7.1. These files will overwrite the old xsdir files during installation. It is the intent of the mcnp development team to prevent users from using the wrong data tables, so older copies of these files will also be overwritten during the installation of the beta.ments on using the Beta release for Criticality Safety applicationsMCNP6.1 is the new production version of MCNP [1] released in June 2013. An updated betaversion, MCNP6.1.1, is targeted for release in summer 2014 to enable the use of several newfeatures for homeland security and nonproliferation applications. The beta version does not include new capabilities for the criticality safety community, but does run significantly faster than theproduction version. To verify that both MCNP6.1 and MCNP6.1.1 are performing correctly for criticality safety applications, several suites of verification/validation benchmark problems were runin early 2014. Detailed results and discussion are provided in LA-UR-14-22480, and summarized here:The general conclusions from the recent testing of MCNP6.1 and MCNP6.1.1 for criticality safety applications are:* Both MCNP6.1 and MCNP6.1.1 perform correctly for criticality safety applications.* While small differences were noted for a few cases, these are strictly due to computer roundoff and are not a concern for verification/validation.* MCNP6.1 runs roughly 20-30% slower than MCNP5-1.60.* MCNP6.1.1 runs at least 50-70% faster than MCNP6.1 and 10-15% faster than MCNP5- 1.60.Criticality safety analysts should consider testing MCNP6.1 or MCNP6.1.1 on their particularproblems and validation suites, to prepare for the migration from MCNP5 to MCNP6. It is expected that this migration should be accomplished within the next 1-3 years.F.B. Brown, B.C. Kiedrowski, J.S. Bull, "Verification of MCNP6.1 and MCNP6.1.1 for CriticalitySafety Applications", LA-UR-14-22480 (2014).VII.Known IssuesThe following is a list of known, higher-priority bugs:artf31853 : Nested dxtran spheres in a lattice caused bad trouble error.artf28495 : LNK3DNT issues with eigenvalue calculationsartf29933 : Infinite loop in sdef cell sampling in a latticeartf29268 : Questionable posting to TALMESH and/or TALHEAT manifesting in PHL special tally treatmentartf26747 : Protons on He3 cause array-out-of-bounds error in array XSSartf28325 : Mix-and-match transport below tabular limits.artf28042 : mesh tally isotopic production feature results include reactions in a voidartf10665 : Coincident surface errors using tr in fillartf27093 : Conversion of particle type from LAQGSM to mcnp causes MPI segmentation error artf18740 : Mesh tally number of tracks calculation incorrect, using FM option -1artf25475 : lost particles in a magnetic field latticeVIII.Works in Progressa.Adding the capability to transport light ion source particles (1H, 2H, 3He, 4He, 6Li, 7Li) onto thesame light ion targets, using nuclear and atomic data tables, not theoretical models. The models can be wrong by orders of magnitude for threshold nuclear reactions. This capability is stillunder development and requires the user to download the nuclear data file (CP2011) and updated xsdir, which will be made available from the webpage in the near future. Awarning message will be issued by the code if the particle falls below the lowest energy of thedata table. The light ion tables are only for the source particle onto a heavier particle (thus there is no table for 7Li onto 2H). artf27087b.The charged particle light ion data tables, ACE library CP2011, are not yet available at the timeof this beta release, but will be made available shortly on the website. This data comes from both LANL and TENDL evaluations. See “The Los Alamos CP2011 ACEFormat Charged Particle Transport Library for MCNP6”, by D. Kent Parsons and Morgan C.White.c.Correct elevation scaling for the cosmic background source capability is under development. IX.Changes in running the code.a.MCNP6 can now print out version information to the screen when the –v is added to thecommand prompt line. Artf30585X.Provided Executable Compilation DetailsThe mcnp v 6.1.1 beta executable for Linux was built with:Intel Fortran 12.1.5Gcc 4.7.xCONFIG= “intel plot omp”64-bit executable (x86_64 arch)The mcnp v 6.1.1 beta executable for Linux was built with:Windows Server 2008 Release 2 Standard – Service Pack 1, with AMD-64 bit CPUsIntel Fortran 12.1.7Gcc 4.7.xCONFIG= “intel plot omp”64-bit executable (x86_64 arch)The mcnp v 6.1.1 beta executable for Mac was built with:Mac OS X 10.6.8 (supported on 10.6 & higher)Intel Fortran 12.0gcc 4.2.1CONFIG="intel plot omp"64-bit executable (x86_64 arch)XI.Installing InstructionsThe directions for installing MCNP6v1.1 are found in the HTML file“ABOUT_MCNP611.html”, located in the top level directory on the MCNP 4th DVD (ie. theDVD specific to the MCNP6.1.1 beta release). If you do not already have MCNP6v1 installed, you need to install it first (using RSICC DVDs 1,2 and 3).。
Moxa ThingsPro 1 产品介绍说明书
IIoT DAQ software platform with built-in AWS IoT Device SDK Amazon’s AWS IoT (https:///iot-platform/) is aleading Internet of Things web service that provides users with a cloud platform and services for building IoT applications. Amazon not only manage’s your cloud storage infrastructure, they also make it easy for you to work with your connected devices and the data generated by those devices. Amazon’s AWS Greengrass (https:///greengrass/) software provides network security for managing connected devices.Moxa provides an easy-to-use web interface and RESTful API for use with Moxa’s ThingsPro Software Suite, which can be integrated with AWS IoT to offer an intelligent data acquisition and device management software platform.IntroductionThingsPro Gateway for AWS IoT›Modbus polling engine with easy-to-use web-based UI for dataacquisition from end devices in an industrial automation field site ›Supports the MQTT IoT protocol to connect fieldbus data to your cloud-based IoT application›Built-in AWS IoT Device SDK with web interface to accelerate your IoT application development›Wireless manager handles the task of connecting your field devices to a wide area network (WAN)›RESTful API and Modbus API for access to all the softwarefunctions of ThingsPro GatewayMoxa’s ThingsPro Gateway is an IIoT software platform that makes data acquisition easy by providing a web interface for configuring and managing Modbus data acquisition, LTE connectivity, and MQTT communication. This software turns eligible Moxa edge computers into intelligent edge platforms that greatly reduce the effort needed to develop IIoT applications. With ThingsPro Gateway, you can build an edge-to-cloud data transmission and process platform that not only brings your raw or processed edge data back to your enterprise or cloud database, but also allows you to focus on developing software with Moxa’s RESTful API or Modbus API to build a smarter field site. In addition, you may install the AWS Greengrass Core directly on your IIoT Gateway Starter Kit for use with ThingsPro Gateway.Moxa Tech Notes for ThingsPro:• How to Build an IoT Application with Moxa’s ThingsPro and AWS IoT Servicehttps:///doc/tech_notes/Moxa_Tech_Note---ThingsPro_AWS_IoT_Application.pdf• Getting Started with AWS IoT on Moxa UC-8100-LX Using Embedded C SDKhttps:///doc/tech_notes/Moxa_Tech_Note---ThingsPro_AWS_IoT_Application.pdf• How to Use ThingsPro Gateway to Publish Processed Edge Data to AWS IoThttps:///doc/tech_notes/Moxa_Tech_Note---How_to_Publish_Processed_data_to_AWS_IoT.pdfUseful AWS Resources:• AWS IoT SDKs and Tools https:///tools/• AWS Green Grass SDKs and Toolshttps:///documentation/greengrass/Additional Resources• Build Modbus device templates that can be used anywhere you want• Use Tag editor to set up unique identifiers for I/O points • Unlimited tag support • Custom tag management• Set up a data polling table with your desired time interval and upload logs to a cloud database or application• json, xml, and csv upload profiles supported• Set up and monitor cellular strength • Supports keep-alive checks• Supports DHCP Server for Ethernet ports•Supports OpenVPN client to build a secure tunnel for data transmission to a remote server• Set up port-forwarding rules to create a simple firewall• Configure system settings such as Time, GPS, Serial Ports, and GPS location though a web UI• Import system configuration from another ThingsPro Gateway• Enable Modbus Slave to allow your local SCADA system to poll data from end devices that are attached to ThingsPro Gateway• Set up an MQTT client to pub/sub your acquired data to/from any MQTT broker• Supports other MQTT-based third party services• Modbus API via C and Python provides access to ready-to-run data acquisition software functions• High-level RESTful API allows you to retrieve or set ThingsPro Gateway device status and resources for better interoperability• It’s easier than ever to enhance or extend ThingsPro Gateway functions and integrate them into your software applicationImportant FeaturesOrdering InformationThingsPro-GW:eligible device.The AWS IoT Device SDK and a web interface are integrated into ThingsPro Gateway to make it easier for users to configure and use the software.Note: ThingsPro Gateway supports the listed firmware versions or higher.Note: Additional series/models will be supported as ThingsPro Gateway is updated. Check Moxa’s website for the most up-to-date information.IIoT Gateway Starter KitUC-8112-LX-STK: UC-8112-LX development kit with ThingsPro Gateway software (ThingsPro-GW) pre-installedNote: Each account may order a maximum of two IIoT Gateway Start Kits.The AWS IoT Device SDK and a web interface are integrated into ThingsPro Gateway to make it easier for users to configure and use the software. In addition, the kernel configuration and required software packages are pre-installed to meet the technical dependencies of the AWS Greengrass Core.。
科罗曼特·卡普托 刀具选择手册说明书
For more technical information, see ourMetalcutting Technical Guide.Symbols for page references:How to choose tool, overview Toolholding Systems, overviewSpare parts/accessories Conversion table, formulas and definitionsrequirements. Use the program overview to find a suitableG 3TOOLING SYSTEMSTooling guideTurning centersG10ProductsFor turning centers - non-rotating toolsCoromant Capto ®G4Toolholder overview G6Manual clamping units G11Automatic clamping unitsG23Boring bar adapterG26Extension/reduction adaptersG28Coromant Capto ® short adapter for exchangeable headG31ER-collet chuck adapter, shortG32Tap adapterG33Spare parts and accessoriesG35CoroTurn ® SL flexible system I1Tools for Multi-Task machiningH1Tooling systems for rotatingSee main catalog for milling, drilling and boring tools.G 4Coromant Capto® can be integrated directly into the turret using the standard clamping mechanism.Standard lathes can easily be converted to Coromant Capto® quick change tools using standard clamping units.Coromant Capto Disc Interface New solution to replace VDI turretsSame adapters fit multiple machine brands and models Cam actuation screw inside turretCoromant Capto ’Bolt on’ Interface ’Bolt on’ solutionUnique hole pattern for each machine brand Cam actuation screw-in bolt on unitSquare shank Round shank Square shank Round shank Special VDI angled VDI straightCx-391.01Cx-391.02Page G28G29Boring bar adapter Boring bar forMulti-TaskRadial AxialmountingAxialmountingAngular AxialmountingBlanks forcutting units 1)Round shank tools, see General turning,Parting and grooving and ThreadingShank tools, see General turning, Parting and grooving andSteel shank Dampened boringbarsCx-A570/Cx-570CoroTurn® SL cutting heads, see General turning, Parting and grooving, Threading, CoroTurn SL and Multi-task machining chapters.Clockwise spindle rotationExternal machiningInternal machiningCounterclockwise spindle rotationApplication guide for type 2000 and 3000A cover plug (CX-CP-01) must be used in a clamping unit when no cutting unit is installed. As can be seen from the diagram and table, there is no Clamping unit CX-R/LC 2085Upper turretClamping unit NC2000/3000 Upside downRight hand cutting unitsClamping unit NC2000/3000Lower turretUpper turretClamping unit NC2000/3000Left hand cutting unitsClamping unit NC2000/3000 Upside downLower turretNote: Maximum coolant pressure is 80 bar (PSI 1160)Metric versionNote: Maximum coolant pressure is 80 bar (PSI 1160)Metric versionG48G6G2J2Metric version G47G6G2J2Right hand style shown. G49G6G2J2Note: Maximum coolant pressure is 80 bar (PSI 1160)G51G6G2J2Metric versionNote: Maximum coolant pressure is 80 bar (PSI 1160)Dimensions Clamping unit23h24l21min l22l23l24l2519.5 1.58.539191933.527.5 2.51143191936.5 G50G6G2J2Clockwise spindle direction Counterclockwise spindle directionNote: Polygon sleeve must be turned 180°. SeeMetalcutting technical guide.Note: Polygon sleeve must be turned 180°. SeeMetalcutting technical guide.Clockwise spindle direction Counterclockwise spindle directionRight hand style shown. Note: Maximum coolant pressure is 80 bar (PSI 1160)Left hand style reflected.Right hand style shown.Left hand: hexagon clamping socket on opposite side of the tool. Note: Maximum coolant pressure is 80 bar (PSI 1160)G52G6G2J2CDI is compatible mainly with European machine tools and follows the standard turret configuration of many lathe producers. CDI also fits multi-task machines having a lower turret with static and/or driven tools.The CDI turret system is designed for Coromant Capto® quick change tooling systems.The design of CDI with short swing diameter provides added space for tool changing, longer tools, or biggerG55G6G2J2Right hand style shown.Right hand style shown. G54G6G2J2Regarding detailed build-in instructions see the Metalcutting Technical guide, and/or contact your local Sandvik Coromant office.D m min Type NC5010 for coolant pressure up to 80 bar (1160 PSI).Type NC5110 for coolant pressure up to 80 bar (1160 PSI), and equipped with probe contactsG56G6G2J2D m minNeutral style shown. Type NC5210 for coolant pressure over 100 bar (1450 PSI).G57G6G2J2DimensionsCoolant1)dmDimensions, inchCoolant1)dmG59G6G2J2l7in.mm4.3314.921110 G6G2J2CoolantC3-391.01-32 060ACoupling sizeCoolantC3-391.01-32 035G60G6G2J2Design 1Design 2Dimensions, millimeter, inch (mm, in.)Ordering code Coolant1)D mm140Note!G60G6G2J2Coolant1G60G2J2KeyG6G2J2Short designNote: For segment clamping only. Cannot be used in basic holders and extensions/ G61G6G2J2Note: Only for segment clamping.l1 = programming lengthTap shank dimensions, Dimensions, mm, inch391.60A-OZ J421G62G6G2J2TOOLING SYSTEMS Index for accessories and spare partsB G 35G e n e r a l T u r n i n g P a r t i n g a n d G r o o v i n gC T h r e a d i n g M u l t i -t a s k m a c h i n i n g I C o r o T u r n ® S L JG en e ra linf o r mat i on AH G T o o l i n g s y s t e m s Index for accessories and spare parts TOOLING SYSTEMSAccessoriesPage FixturesG36Grease nipple G38Push button operated valveG38Assembly toolG38Hexagon keyG38Torque wrench for tool changingG39Tightening torque recommendationsG40Extracting tool for clamping unitsG41Tools for disassembling polygon socketG41Cover plugG41Cassettes with tapered polygon seatingG43Coolant tubes for Coromant Capto ®G42Spare partsPage Manually operated clamping unitsType 2085G47Type 2000G48Type 3000G48Type 2080G48Type 2090G50Round shank typeG51VDI Angled design, DIN 69880G52VDI Straight design, DIN 69880G53CDI straightG54CDI angledG55Hydraulically operated clamping unitsType 5010G56Type 5110G58Type 5210G57Coromant Capto ® adaptersCoromant Capto ® adapter for solid boring barG59Extension/reduction adaptersG60Collet chuck adaptersG61Tap adapters G62Page Pre-measuring fixture G45Master setting gaugesG46Alignment tool G46For spare parts for Coromant Capto ® Multi-Task machining tools, see chapter H.Ordering code: 391.500Sleeve Collar391.540-C3391.530-C3Choose flange, collar and sleeve to suit tool to be Choose sleeve to suit coupling.The fixture should be fastened to the bench with threesocket head screws (not delivered with fixture).l x1l x2l y1l y2 6713510015105 2.637 5.315 3.737.590 4.134Assembly toolTorque wrenchExtracting tool Withdrawal toolSafe control-4000Plastic storage cassettes (red)-5000High grade plastic in-machine tool storage (black)Passive locking mechanism Active locking mechanismCenter height gauge Alignment tool Tool post center lineTool presetting Component fixture geometric controlThe spring, item 15g, should be used 1123Size Drawbar Clamping screw Holder set (set of 2)C35461 105-015519 105-015546 002-01 C45461 105-025519 105-025546 002-0212The spring, item 17g, should be used onlywhen mounting draw bar set in old designclamping units, where the spring must beplaced inside the plain bearing as shown15678Plain bearing Locking washer Screw Plain bearing Cam shaft3823 010-******* 030-01416.1-8345638 022-015333 025-013823 010-******* 030-02416.1-8345638 022-025333 025-02when mounting a draw bar set in old designplaced inside the plain bearing as shown in1567Plain bearing Locking washer Screw Plain bearing3823 010-******* 030-01416.1-8345638 022-01draw bar set in old designplaced inside the plain bearing as shown in1567Plain bearing Bushing Locking washer Screw3823 010-******* 024-015541 030-01416.1-8343823 010-******* 024-025541 030-02416.1-834。
Moxa ioLogik 4000 Series模块化远程I O系统说明书
ioLogik4000SeriesModular remote I/OFeatures and Benefits•I/O expansion without a backplane•Active communication with MX-AOPC UA Server•Supports SNMP v1/v2c•Easy configuration with Modular ioAdmin utility•Friendly configuration via web browser•Simplifies I/O management with MXIO library for Windows or LinuxCertificationsIntroductionThe ioLogik4000Series is suitable for remote monitoring and alarm systems,such as those used for water treatment systems,water supply systems,wastewater treatment systems,and power monitoring systems.These kinds of applications need more I/O points and a variety of I/O types,including temperature sensors,gas detectors,and water quality detectors,all of which can benefit from the versatile mixture of I/O features supported by the ioLogik4000Series.Slice Form Factor and Flexible I/O VarietyThe unique modular construction of the ioLogik4000Series allows for mixing and matching of modules to achieve the best combination of I/O modules to meet the needs of a wide range of remote automation applications.An industrial modular housing enables I/O modules to be added to the base unit without a backplane.The ioLogik4000Series is perfect for space-limited applications,and high-density I/O points are provided for greater flexibility and expandability.Modules can connect to virtually any type of sensor,including but not limited to those for temperature, pressure,flow,voltage,current,and contact closure.Easy MaintenanceThe ioLogik4000Series comes with removable spring-type terminal blocks(RTBs)that allow you to conserve field wiring for future use.SpecificationsInput/Output InterfaceButtons Reset buttonControl LogicLanguage ioLogik E4200:Click&Go,NA-4010/4020/4021:NoneEthernet Interface10/100BaseT(X)Ports(RJ45connector)NA-4010:1ioLogik E4200:2,2MAC addressesEthernet Software FeaturesConfiguration Options NA-4010/ioLogik E4200:Web Console(HTTP)NA-4010/4020/4021:Windows Utility(ioAdmin)ioLogik E4200:Windows Utility(Modular-ioAdmin)Industrial Protocols NA-4010:Modbus TCP Server(Slave),MXIO LibraryNA-4020/4021:MXIO LibraryioLogik E4200:Modbus TCP Server(Slave),Moxa AOPC(Active Tag),MXIO Library,SNMPv1/v2c,SNMPv1TrapManagement NA-4010/ioLogik E4200:DHCP Client,HTTP,IPv4,TCP/IP,UDPNA-4010/4020/4021:ioAdminioLogik E4200:Modular-ioAdminSerial InterfaceConnector NA-4021:DB9femaleNA-4020:Terminal blockData Bits7,8No.of Ports1Parity None,Even,OddSerial Standards NA-4021:RS-232NA-4020:RS-485Stop Bits1,2Baudrate NA-4020/4021:1200,2400,4800,9600,19200,38400,57600,115200bpsSerial Software FeaturesIndustrial Protocols NA-4020/4021:Modbus RTU/ASCII SlaveSerial SignalsRS-232NA-4021:TxD,RxD,GNDRS-485-2w NA-4020:Data+,Data-,GNDDIP Switch ConfigurationSerial Interface Fixed baudrateModbus TCPMax.No.of Client Connections8Mode Client(Master)Power ParametersNo.of Power Inputs1System Power ParametersPower Connector Spring-type Euroblock terminalNo.of Power Inputs1Input Voltage11to28.8VDCPower Consumption NA-4010:60mA@24VDCNA-4020/4021:70mA@24VDCioLogik E4200:175mA@24VDCField Power ParametersPower Connector Spring-type Euroblock terminalNo.of Power Inputs1Input Voltage11to28.8VDCCurrent in Field Power Contact10A(max.)Physical CharacteristicsHousing PlasticDimensions45x99x70mm(1.77x3.90x2.76in)Weight NA-4010/4020/4021:150g(0.33lb)ioLogik E4200:180g(0.396lb)Installation DIN-rail mountingEnvironmental LimitsOperating Temperature-10to60°C(14to140°F)Storage Temperature(package included)-40to85°C(-40to185°F)Ambient Relative Humidity5to95%(non-condensing)Altitude2000m1Standards and CertificationsEMC EN61000-6-2/-6-4EMI CISPR22,FCC Part15B Class AEMS IEC61000-4-2ESD:Contact:4kV;Air:8kVIEC61000-4-3RS:80MHz to1GHz:10V/mIEC61000-4-4EFT:Power:2kV;Signal:1kVIEC61000-4-5Surge:Power:2kV;Signal:1kVIEC61000-4-6CS:10VIEC61000-4-8PFMFSafety UL508Shock IEC60068-2-27Vibration IEC60068-2-6DeclarationGreen Product RoHS,CRoHS,WEEEMTBFTime NA-4010:4,739,300hrsNA-4020:4,721,640hrsNA-4021:4,695,360hrsioLogik E4200:357,000hrsStandards Telcordia SR332WarrantyWarranty Period2yearsDetails See /warranty1.Please contact Moxa if you require products guaranteed to function properly at higher altitudes.Package ContentsDevice1x ioLogik4000Series remote I/OInstallation Kit1x terminal block,8-pin,3.81mmDocumentation1x quick installation guide(ioLogik E4200)1x warranty cardNote This product requires additional modules(sold separately)to function.DimensionsI/O Network Adapter I/O ModuleOrdering InformationModel Name Control Logic Ethernet Interface Serial Interface No.of Support I/O Modules ioLogik E4200Click&Go2x RJ45–16NA-4010–1x RJ45–32NA-4020––RS-48532NA-4021––RS-23232 Accessories(sold separately)I/O ModulesM-1450For the ioLogik4000Series,4DIs,110VACM-1451For the ioLogik4000Series,4DIs,220VACM-1600For the ioLogik4000Series,16DIs,24VDC,sink typeM-1601For the ioLogik4000Series,16DIs,24VDC,source typeM-1800For the ioLogik4000Series,8DIs,24VDC,sink typeM-1801For the ioLogik4000Series,8DIs,24VDC,source typeM-2450For the ioLogik4000Series,4relays,24VDC/230VAC,2AM-2600For the ioLogik4000Series,16DOs,24VDC,0.5A,sink typeM-2601For the ioLogik4000Series,16DOs,24VDC,0.5A,source typeM-2800For the ioLogik4000Series,8DOs,24VDC,0.5A,sink typeM-2801For the ioLogik4000Series,8DOs,24VDC,0.5A,source typeM-3802For the ioLogik4000Series,8AIs,4to20mA,12bitsM-3810For the ioLogik4000Series,8AIs,0to10V,12bitsM-4402For the ioLogik4000Series,4AOs,4to20mA,12bitsM-4410For the ioLogik4000Series,4AOs,0to10V,12bitsM-6200For the ioLogik4000Series,2AIs,RTD:PT100,JPT100M-6201For the ioLogik4000Series,2AIs,TCPower ModulesM-7001For the ioLogik4000Series,system power moduleM-7002For the ioLogik4000Series,field power moduleM-7804For the ioLogik4000Series,potential distributor module,0VDC8channelM-7805For the ioLogik4000Series,potential distributor module,24VDC,8channelSoftwareMX-AOPC UA Server OPC UA Server software for converting fieldbus to the OPC UA standard©Moxa Inc.All rights reserved.Updated Dec09,2020.This document and any portion thereof may not be reproduced or used in any manner whatsoever without the express written permission of Moxa Inc.Product specifications subject to change without notice.Visit our website for the most up-to-date product information.。
1732 ArmorBlock 诊断16点I O模块系列A用户指南说明书
Wiring Diagrams Pinout Guide for 1732 ArmorBlock Diagnostic 16-point I/O Modules, Series ACatalog Numbers 1732D-8X81212D, -8X81212HD,-8I8O1212D, -IB161212D, -IBDPM12MND, -8X8M12D Package ContentsYour package contains one 1732 ArmorBlock module and these wiring instructions.For complete installation instructions, refer to the Module Publication Number table and visit .Module Publication NumberModule PublicationNumber1732D-IN009 DeviceNet 1732 ArmorBlock Network-powered 8-input Auxiliary-powered8-output Module, Series A (cat. no. 1732-8X81212D)1732D-IN010 DeviceNet 1732 ArmorBlock Network-powered 8-input/8-output Module,Series A (cat. no. 1732-8I8O1212D)1732D-IN011 DeviceNet 1732 ArmorBlock Network-powered 8-input Auxiliary-powered8-output Module, Series A (cat. no. 1732-8X81212DH)1732D-IN012 DeviceNet 1732 ArmorBlock Network-powered 16-input Module, Series A(cat. no. 1732-IB161212D)1732D-IN007 DeviceNet 1732 ArmorBlock Network Powered 16-Input Module, Series A(cat. no. 1732D-IBDPM12MND)1732D-IN004 DeviceNet 1732 ArmorBlock Network-powered 8-input Auxiliary-powered8-output Module, Series A (cat. no. 1732D-8X8M12D)Publication 1732-WD003C-EN-P - July 20092 Pinout Guide for 1732 ArmorBlock Diagnostic 16-point I/O Modules, Series A Publication 1732-WD003C-EN-P - July 2009DeviceNet ConnectorsI/O Connectors1732 DeviceNet Micro-style(view into connector)Pin 1DrainPin 2V+Pin 3 V-Pin 4CAN_HPin 5CAN_L (view into connector)Pin 1DrainPin 2V+Pin 3V-Pin 4CAN_HPin 5CAN_LNetwork-in Male Connector Network-out Female Connector1732 DeviceNet Mini-styleNetwork-in Male Connector(view into connector)Pin 1 Sensor Source VoltagePin 2 Input BPin 3 ReturnPin 4 Input APin 5 PE 5-pin Female Input Micro-stylePinout Guide for 1732 ArmorBlock Diagnostic 16-point I/O Modules, Series A 3Publication 1732-WD003C-EN-P - July 2009Auxiliary Power Connector ATTENTION Two sets of mounting holes are used to mount the module directly to a panel or machine. Mounting holes accommodate #6 (M3) pan head screws. The torquespecification is 0.64 Nm (6 in-lb).ATTENTION This equipment is considered Group 1, Class A industrial equipment accordingto IEC/CISPR Publication 11. Without appropriate precautions, there may bepotential difficulties ensuring electromagnetic compatibility in otherenvironments due to conducted as well as radiated disturbance.(view into connector)Pin 1 Not UsedPin 2 Output BPin 3 ReturnPin 4 Output APin 5 PE5-pin Female Output Micro-style(view into connector)Pin 1Sensor Source VoltagePin 2 OutputPin 3 ReturnPin 4 InputPin 5 PE5-pin Female Combination Micro-style(view into connector)Pin 1Output Power+Pin 2No ConnectionPin 3No ConnectionPin 4Output Power-4-pin Mini-style4 Pinout Guide for 1732 ArmorBlock Diagnostic 16-point I/O Modules, Series A Publication 1732-WD003C-EN-P - July 2009SpecificationsEnvironmental Specifications AttributeValue Temperature, operating-20…60 °C (-4…140 °F)Ambient temperaturerating (UL)60 °C (140 °F)Operating voltage11…30V DC Output current 1732D-8I8O1212D - 0.5A per output1732D-8X81212D - 0.5A per output1732D-8X81212HD - 1.4A per output1732D-8X8M12D - 0.5A per outputEnclosure type ratingMeets IP65/66/67/69K (when marked), and NEMA 4X/6P withreceptacle dust caps or cable termination.CertificationsCertifications (whenproduct is marked)(1)(1)See the Product Certification link at for Declarations of Conformity, Certificates, and other certification details.Value c-UL-usUL Listed Industrial Control Equipment, certified for US and Canada. See UL File E322657CE European Union 2004/108/EC EMC Directive, compliant with:EN 61326-1; Meas./Control/Lab., Industrial RequirementsEN 61000-6-2; Industrial ImmunityEN 61000-6-4; Industrial EmissionsEN 61131-2; Programmable Controllers (Clause 8, Zone A & B)C-Tick Australian Radiocommunications Act,compliant with: AS/NZS CISPR 11; Industrial EmissionsDeviceNetODVA conformance tested to DeviceNet specificationsPinout Guide for 1732 ArmorBlock Diagnostic 16-point I/O Modules, Series A 5 Notes:Publication 1732-WD003C-EN-P - July 20096 Pinout Guide for 1732 ArmorBlock Diagnostic 16-point I/O Modules, Series A Notes:Publication 1732-WD003C-EN-P - July 2009Pinout Guide for 1732 ArmorBlock Diagnostic 16-point I/O Modules, Series A 7 Notes:Publication 1732-WD003C-EN-P - July 2009Publication 1732-WD003C-EN-P - July 2009PN-51088Supersedes Publication 1732-WD003B-EN-P - January 2008Copyright © 2009 Rockwell Automation, Inc. All rights reserved. Printed in Singapore.Rockwell Automation SupportRockwell Automation provides technical information on the Web to assist you in using its products. At , you can find technicalmanuals, a knowledge base of FAQs, technical and application notes, sample code and links to software service packs, and a MySupport feature that you can customize to make the best use of these tools.For an additional level of technical phone support for installation, configuration and troubleshooting, we offer TechConnect Support programs. For more information, contact your local distributor or Rockwell Automation representative, or visit .Installation AssistanceIf you experience a problem with a hardware module within the first 24 hours ofinstallation, please review the information that's contained in this manual. You can also contact a special Customer Support number for initial help in getting your module up and running:New Product Satisfaction ReturnRockwell tests all of its products to ensure that they are fully operational when shipped from the manufacturing facility. However, if your product is not functioning and needs to be returned:Allen-Bradley, Rockwell Automation, TechConnect, and ArmorBlock are trademarks of Rockwell Automation, Inc. Trademarks not belonging to Rockwell Automation are property of their respective companies.United States1.440.646.3434 Monday – Friday, 8am – 5pm EST Outside United States Please contact your local Rockwell Automation representative for any technical support issues.United States Contact your distributor. You must provide a Customer Support case number (see phone number above to obtain one) to your distributor in order to complete the return process.Outside United StatesPlease contact your local Rockwell Automation representative for returnprocedure.。
霍巴特AM3洗盘替换零部件手册说明书
19
18
21
20
19
18
9
13
14 15 12 16 17
AM3 DISHWASHER REPLACEMENT PARTS
LOWER FRAME ASSEMBLY
PART NO.
NAME OF PART
1
743388 1.1 kW Wash Pump For 380/50/3 220/50/3
9
RR-11-03 Retaining Ring
10
743309 Throttle Plate
11
743399 Lower Manifold/Shaft Assy
12
473984 Gasket- Manifold
13
473460 Nut- Manifold
14
67500-10 "O" Ring 22.4*3.55
3
538412 Clamp- Hose 50mm
4
542337 Clamp- Hose 60mm
5
743315-1 Clamp- Hose 75mm
6
743396 Hose Adapter- Wash Pump Inlet
7
743336 Clamp- Hose 14mm
8
743397 Piping- Wash Pump Drain
22
743446-3 End Cap- Rinse Arm
AMT.
1 1 4 20 12 2 2 1 1 1 1 1 1 3 1 4 1 20 16 2 2 4
6
AM3 DISHWASHER REPLACEMENT PARTS
WASH TANK
211A_Manual说明书(1)
User’s Manual
Model 211
Temperature Monitor
Serial Numbers 21A0000 and Subsequent
TESCOM TC-5901B 胶囊式电磁干扰盾盒说明书
TC-5901B Pneumatic Shield BoxApplicationTC-5901B Pneumatic Shield Box is about 50% larger in test area andheight than our popular TC-595X series shield boxes providing moreflexibility in handling larger devices and test fixtures. This productprovides an efficient RF-isolated test environment for manufacturingmobile phones and wireless PDA’s.Key Features! Reliable High RF shielding up to 5GHz owing to simple up-down lid motion! Pneumatic control of lid open-close and optional automatic test fixture motions! EMI filters on all data ports and power line! Manual or remote operation(RS-232C)! Modular I/O design for possible customization of data and RF connection! Fixture control air outputs for custom test fixture! Red and Green LED ‘s for Pass/Fail indication! Dual pressure control and Lid Down Sensor for safety! CE compliant : EN 292-1:1991, EN 292-2:1991/A1:1995, EN 983:1996, EN 1050:1996, EN 60204-1:1997Specification! Shielding Effectiveness : >70 dB, from DC to 2.5 GHz,including connectors! Remote control : RS-232C, 3 wire, DB-9 M! Input air pressure : 5bar to 10 bar! Main air connector : 6mm OD hose, one-touch push-on fitting! Fixture control air connectors : 4mm OD, one-touch push-on fitting! Line voltage : 100 to 240 VAC, 50/60 Hz, 15 watt max! Working space : 408(W) x 218(D) x 135(H) mm! Dimension : 459(W) x 300(D) x 302(H) mm, lid closed. 449(H) mm, lid open! Weight without optional test fixture : 20 kgLid Characteristics! Long Life Up-Down design! Lid Speed Control Valve! Dual Pressure : Lid closes without air pressure! Lid Down Sensor: Lid opens if the lid doesn’t close fully within 2 sec.! Initial Lid Position : Lid opens when the air is connected regardless of AC power! Internal Electronic Cycle Counter (downloadable to PC thru RS-232C)Electrical I/OUser Data I/O Connectors! DATA #1, #2 : DB9-P outside, DB9-S inside, 1000pF Pi filter! DATA #3 : DB25-P outside, DB25-S inside, 1000pF Pi filter! DATA #4 : DB9-P outside, DB9-S inside, for USB 2.0 or 100MBps LAN, 100pF Pi filterRF Connectors! RF #1,#2 : N-type outside, SMA insideRemote Control Connector! RS-232C : DB9-PAC Line Input! IEC Type, DetachableFixture CustomizationTC-5901B system with a custom fixture TC-59014A allows automation of most of the test processes. The fixture holds the device, brings it inside the shield box, makes test signal connections and returns the device to the starting position when the test is completed. Once the device is manually loaded on the DUT cradle and the serial number scanned, the rest of the test procedures can be automated. The custom fixture option, TC-59014A may be ordered through Tescom by supplying the necessary device information and samples to help design and verification. Typically, every device has unique shape and electrical connections requiring mechanical and electrical customizations from its basic platform design.A sample design is shown below.TC-59014A Typical Fixture Fixtures fittedOrdering InformationTC-5901B, Pneumatic Shield BoxTC-59014A, Custom Fixture for 5901B5901-71, Dual open/close switch5901-80, Spare gasket Assy, set of 2 pcsDesign Patent 240189SPECIFICATIONS SUBJECT TO CHANGE WITHOUT PRIOR NOTICE View of TC-59014A fixture。
Molex MXS连接器商品说明书
NOTES:1. MATERIALS: HOUSING: LIQUID CRYSTAL POLYMER (LCP) GLASS-FILLED, UL94V-0 TERMINALS: HIGH PERFORMANCE COPPER ALLOY2. FINISH: SELECTIVE GOLD IN SIGNAL AND U-SHIELD CONTACT AREA ONLY. SELECTIVE TIN ON PCB TAILS. NICKEL OVERALL.3. PARTS ARE MAKED WITH THE PART NUMBER, "MXS", AND A DATECODE.DATECODE FORMAT: 2 DIGIT YEAR, 3 DIGIT DAY (YYDDD), AND 2 DIGIT HOUR, MINUTE & SECONDS (HHMMSS).4. REFER TO MOLEX PRODUCT SPECIFICATION 1735109000-PS_PS FOR PERFORMANCE SPECIFICATIONS.5. PRODUCT WILL BE PACKAGED PER: 1736101000PDD_PK.6. MATES WITH IMPULSE DC RAF (RIGHT ANGLE FEMALE) UNGUIDED NUMBER: SEE TABLE.7. APPLICATION SPECIFICATION: 1735109997-AS_PS.8. REFER TO IMPULSE PCB ROUTING GUIDE 1735109999-AS_PS FOR ANTIPAD,ROUTING RECOMMENDATIONS, BACKDRILL, AND ADDITIONAL PCB INFORMATION.CA57.6REFERENCE19.10.62X8.5437.435.0MIN BOARD1.00TAIL LENGTH1.3012.07(CUTAWAY)2.37PIN T16PIN A116 COLUMN PART SHOWNPART NUMBER "MXS" DATECODE SEE NOTE 3MATERIAL NUMBER NUMBER OF COLUMNS NUMBER OF DIFFERENTIAL PAIRS DIMENSION "A"DIMENSION "B"DIMENSION "C"DIMENSION "D"ORTHOGONALORIENTATIONMATING DC RAF PART NUMBER PTH's173625-280586422.814.0024.015.4890°173540-70080.31MM 173625-2205129630.822.0032.023.4890°173631-10120.31MM 173625-24051411234.826.0036.027.4890°173620-10160.31MM 173625-26051612838.830.0040.031.4890°173505-10160.31MM 173625-280686422.814.0024.015.4890°173540-70480.26MM 173625-2206129630.822.0032.023.4890°173631-10520.26MM 173625-24061411234.826.0036.027.4890°173620-10560.26MM 173625-26061612838.830.0040.031.4890°173505-10560.26MM(SHIELD LENGTH)6.50+0.20-0.10REFERENCE25.52PITCH2.00PIN A1REFERENCE22.92 DIVISIONAL SYMBOLSFUNCTIONAL SYMBOLS =0=0=CURRENT REV DESC: ADD 0.26MM COMPLIANT PARTNUMBERSEC NO:675528DRWN:JMENDOZA012021/06/15CHK'D:JMENDOZA012021/09/01APPR:JMENDOZA012021/09/01THIS DRAWING CONTAINS INFORMATION THAT IS PROPRIETARY TO MOLEX ELECTRONIC TECHNOLOGIES, LLC AND SHOULD NOT BE USED WITHOUT WRITTEN PERMISSION DIMENSION UNITS SCALE mm 3:1GENERAL TOLERANCES (UNLESS SPECIFIED)ANGULAR TOL±0.5 °4 PLACES±3 PLACES ±2 PLACES±0.13IMPULSE 8 PAIRORTHO DIRECT RAM - 90° - 2MM UNGUIDEDPRODUCT CUSTOMER DRAWINGDOCUMENT NUMBERDOC TYPE DOC PART REVISIONMML LKKJJHHG GFFEEDDCC BBDETAIL A SCALE 7:1ADKEEP-OUT ZONE SEE NOTE 1TYPICAL2.003.20 3.201.615.471.995.5335.20MAXIMUM 2.702X2.60UNPLATED SCREW HOLE2X2.45±0.05⌀PTH (32 HOLES PER COLUMN)0.31±0.05⌀⌖⌀0.10⌖⌀0.10(FULLY MATED)26.00ORTHO DIRECT RAM HOLE PATTERN(CONNECTOR SIDE)DC RAFREAR (NON-CONNECTOR SIDE)32.132X 8.38EVEN COLUMN DIMENSIONSODD COLUMN DIMENSIONS 0.002.204.006.208.0010.2012.0014.2016.0018.2020.0022.2024.0026.2028.00 1.003.205.007.209.0011.2013.0015.2017.0019.2021.0023.2025.0027.2029.001.003.205.007.209.0011.2013.0015.2017.0019.2021.0023.2025.0027.2029.002.004.206.008.2010.0012.2014.0016.2018.0020.2022.0024.2026.0028.20GND H1G1GND GND D1C1GND GND K1J1GND GND P1N1GND GND F1E1GND GND M1L1GND GND B1A1GNDR1GND GND M2L2GND GND K2J2GND GND P2N2GND GND H2G2GND GND GND D2C2GND GND B2A2GNDGND F2E2GND DC RAF PIN A1PIN T1PIN A1SIGNAL VIA GROUND VIAPCB EDGEDC RAF FIRST GND PIN0.31 SIGNAL & GROUND PTH's16 COLUMN PART SHOWNNOTES1. THESE DIMENSIONS REPRESENT THE AREA NEEDED TOACCOMMODATE CONNECTOR INSERTION AND REPAIR ON THE PCB. THIS IS REFERRED TO AS THE "CONNECTOR KEEP OUT ZONE" AND DOES NOT REPRESENT THE ACTUAL PERIMETER OF THE DC RAF PIN A1DIVISIONAL SYMBOLSFUNCTIONAL SYMBOLS =0=0=CURRENT REV DESC: ADD 0.26MM COMPLIANT PARTNUMBERSEC NO:675528DRWN:JMENDOZA012021/06/15CHK'D:JMENDOZA012021/09/01APPR:JMENDOZA012021/09/01THIS DRAWING CONTAINS INFORMATION THAT IS PROPRIETARY TO MOLEX ELECTRONIC TECHNOLOGIES, LLC AND SHOULD NOT BE USED WITHOUT WRITTEN PERMISSION DIMENSION UNITS SCALE mm 3:1GENERAL TOLERANCES (UNLESS SPECIFIED)ANGULAR TOL±0.5 °4 PLACES±3 PLACES ±2 PLACES±0.13IMPULSE 8 PAIRORTHO DIRECT RAM - 90° - 2MM UNGUIDEDPRODUCT CUSTOMER DRAWINGDOCUMENT NUMBERDOC TYPE DOC PART REVISIONLLKKJJHHGGFFEEDDCC BBDETAIL C SCALE 7:1ADKEEP-OUT ZONE SEE NOTE 1TYPICAL2.003.20 3.201.615.471.995.5335.20MAXIMUM 2.702X2.60⌖⌀0.10UNPLATED SCREW HOLE2X2.45±0.05⌀⌖⌀0.10GROUND PTH (16 HOLES PER COLUMN)0.31±0.05⌀(FULLY MATED)26.00ORTHO DIRECT RAM HOLE PATTERN(CONNECTOR SIDE)DC RAFREAR (NON-CONNECTOR SIDE)32.132X 8.38EVEN COLUMN DIMENSIONSODD COLUMN DIMENSIONS 0.002.204.006.208.0010.2012.0014.2016.0018.2020.0022.2024.0026.2028.00 1.003.205.007.209.0011.2013.0015.2017.0019.2021.0023.2025.0027.2029.001.003.205.007.209.0011.2013.0015.2017.0019.2021.0023.2025.0027.2029.002.004.206.008.2010.0012.2014.0016.2018.0020.2022.0024.2026.0028.20GND H1G1GND GND D1C1GND GND K1J1GND GND P1N1GND GND F1E1GND GND M1L1GND GND B1A1GNDR1GND GND M2L2GND GND K2J2GND GND P2N2GND GND H2G2GND GND GND D2C2GND GND B2A2GNDGND F2E2GND DC RAF PIN A1PIN T1PIN A1SIGNAL VIA GROUND VIAPCB EDGEDC RAF FIRST GND PIN0.26 SIGNAL PTH's & 0.31 GROUND PTH's16 COLUMN PART SHOWNNOTES1. THESE DIMENSIONS REPRESENT THE AREA NEEDED TOACCOMMODATE CONNECTOR INSERTION AND REPAIR ON THE PCB. THIS IS REFERRED TO AS THE "CONNECTOR KEEP OUT ZONE" AND DOES NOT REPRESENT THE ACTUAL PERIMETER OF THE DC RAF PIN A1⌖⌀0.10SIGNAL PTH (16 HOLES PER COLUMN)0.26±0.05⌀DIVISIONAL SYMBOLSFUNCTIONAL SYMBOLS =0=0=CURRENT REV DESC: ADD 0.26MM COMPLIANT PARTNUMBERSEC NO:675528DRWN:JMENDOZA012021/06/15CHK'D:JMENDOZA012021/09/01APPR:JMENDOZA012021/09/01THIS DRAWING CONTAINS INFORMATION THAT IS PROPRIETARY TO MOLEX ELECTRONIC TECHNOLOGIES, LLC AND SHOULD NOT BE USED WITHOUT WRITTEN PERMISSION DIMENSION UNITS SCALE mm 3:1GENERAL TOLERANCES (UNLESS SPECIFIED)ANGULAR TOL±0.5 °4 PLACES±3 PLACES ±2 PLACES±0.13IMPULSE 8 PAIRORTHO DIRECT RAM - 90° - 2MM UNGUIDEDPRODUCT CUSTOMER DRAWINGDOCUMENT NUMBERDOC TYPE DOC PART REVISIONLLKKJJHHGGFFEEDDCC BBMATED DIMENSIONSREFERENCE3.0REFERENCE35.9REFERENCE95.48 PAIR X 16 COLUMN ORTHO DIRECT RAM8 PAIR X 16 COLUMNDC RAFORTHO PIN MAPPING(DC RAF - ORTHO DIRECT RAM)T1-T2T2-R1T3-P2T4-N1T5-M2T6-L1T7-K2T8-J1T9-H2T10-G1T11-F2T12-E1T13-D2T14-C1T15-B2T16-A1R1-R2R2-T1R3-N2R4-P1R5-L2R6-M1R7-J2R8-K1R9-G2R10-H1R11-E2R12-F1R13-C2R14-D1R15-A2R16-B1P1-T4P2-R3P3-P4P4-N3P5-M4P6-L3P7-K4P8-J3P9-H4P10-G3P11-F4P12-E3P13-D4P14-C3P15-B4P16-A3N1-R4N2-T3N3-N4N4-P3N5-L4N6-M3N7-J4N8-K3N9-G4N10-H3N11-E4N12-F3N13-C4N14-D3N15-A4N16-B3M1-T6M2-R5M3-P6M4-N5M5-M6M6-L5M7-K6M8-J5M9-H6M10-G5M11-F6M12-E5M13-D6M14-C5M15-B6M16-A5L1-R6L2-T5L3-N6L4-P5L5-L6L6-M5L7-J6L8-K5L9-G6L10-H5L11-E6L12-F5L13-C6L14-D5L15-A6L16-B5K1-T8K2-R7K3-P8K4-N7K5-M8K6-L7K7-K8K8-J7K9-H8K10-G7K11-F8K12-E7K13-D8K14-C7K15-B8K16-A7J1-R8J2-T7J3-N8J4-P7J5-L8J6-M7J7-J8J8-K7J9-G8J10-H7J11-E8J12-F7J13-C8J14-D7J15-A8J16-B7H1-T10H2-R9H3-P10H4-N9H5-M10H6-L9H7-K10H8-J9H9-H10H10-G9H11-F10H12-E9H13-D10H14-C9H15-B10H16-A9G1-R10G2-T9G3-N10G4-P9G5-L10G6-M9G7-J10G8-K9G9-G10G10-H9G11-E10G12-F9G13-C10G14-D9G15-A10G16-B9F1-T12F2-R11F3-P12F4-N11F5-M12F6-L11F7-K12F8-J11F9-H12F10-G11F11-F12F12-E11F13-D12F14-C11F15-B12F16-A11E1-R12E2-T11E3-N12E4-P11E5-L12E6-M11E7-J12E8-K11E9-G12E10-H11E11-E12E12-F11E13-C12E14-D11E15-A12E16-B11D1-T14D2-R13D3-P14D4-N13D5-M14D6-L13D7-K14D8-J13D9-H14D10-G13D11-F14D12-E13D13-D14D14-C13D15-B14D16-A13C1-R14C2-T13C3-N14C4-P13C5-L14C6-M13C7-J14C8-K13C9-G14C10-H13C11-E14C12-F13C13-C14C14-D13C15-A14C16-B13B1-T16B2-R15B3-P16B4-N15B5-M16B6-L15B7-K16B8-J15B9-H16B10-G15B11-F16B12-E15B13-D16B14-C15B15-B16B16-A15A1-R16A2-T15A3-N16A4-P15A5-L16A6-M15A7-J16A8-K15A9-G16A10-H15A11-E16A12-F15A13-C16A14-D15A15-A16A16-B15PIN MAPA1R16THIS SHEET IS FOR128 DIFFERENTIAL PAIRS ONLYDIVISIONAL SYMBOLSFUNCTIONAL SYMBOLS =0=0=CURRENT REV DESC: ADD 0.26MM COMPLIANT PARTNUMBERSEC NO:675528DRWN:JMENDOZA012021/06/15CHK'D:JMENDOZA012021/09/01APPR:JMENDOZA012021/09/01THIS DRAWING CONTAINS INFORMATION THAT IS PROPRIETARY TO MOLEX ELECTRONIC TECHNOLOGIES, LLC AND SHOULD NOT BE USED WITHOUT WRITTEN PERMISSION DIMENSION UNITS SCALE mm 2:1GENERAL TOLERANCES (UNLESS SPECIFIED)ANGULAR TOL±0.5 °4 PLACES±3 PLACES ±2 PLACES±0.13IMPULSE 8 PAIRORTHO DIRECT RAM - 90° - 2MM UNGUIDEDPRODUCT CUSTOMER DRAWINGDOCUMENT NUMBERDOC TYPE DOC PART REVISIONLLKKJJH HGGFFEED DCC BBORTHO PIN MAPPING(DC RAF - ORTHO DIRECT RAM)M1-T2M2-R1M3-P2M4-N1M5-M2M6-L1M7-K2M8-J1M9-H2M10-G1M11-F2M12-E1M13-D2M14-C1M15-B2M16-A1L1-R2L2-T1L3-N2L4-P1L5-L2L6-M1L7-J2L8-K1L9-G2L10-H1L11-E2L12-F1L13-C2L14-D1L15-A2L16-B1K1-T4K2-R3K3-P4K4-N3K5-M4K6-L3K7-K4K8-J3K9-H4K10-G3K11-F4K12-E3K13-D4K14-C3K15-B4K16-A3J1-R4J2-T3J3-N4J4-P3J5-L4J6-M3J7-J4J8-K3J9-G4J10-H3J11-E4J12-F3J13-C4J14-D3J15-A4J16-B3H1-T6H2-R5H3-P6H4-N5H5-M6H6-L5H7-K6H8-J5H9-H6H10-G5H11-F6H12-E5H13-D6H14-C5H15-B6H16-A5G1-R6G2-T5G3-N6G4-P5G5-L6G6-M5G7-J6G8-K5G9-G6G10-H5G11-E6G12-F5G13-C6G14-D5G15-A6G16-B5F1-T8F2-R7F3-P8F4-N7F5-M8F6-L7F7-K8F8-J7F9-H8F10-G7F11-F8F12-E7F13-D8F14-C7F15-B8F16-A7E1-R8E2-T7E3-N8E4-P7E5-L8E6-M7E7-J8E8-K7E9-G8E10-H7E11-E8E12-F7E13-C8E14-D7E15-A8E16-B7D1-T10D2-R9D3-P10D4-N9D5-M10D6-L9D7-K10D8-J9D9-H10D10-G9D11-F10D12-E9D13-D10D14-C9D15-B10D16-A9C1-R10C2-T9C3-N10C4-P9C5-L10C6-M9C7-J10C8-K9C9-G10C10-H9C11-E10C12-F9C13-C10C14-D9C15-A10C16-B9B1-T12B2-R11B3-P12B4-N11B5-M12B6-L11B7-K12B8-J11B9-H12B10-G11B11-F12B12-E11B13-D12B14-C11B15-B12B16-A11A1-R12A2-T11A3-N12A4-P11A5-L12A6-M11A7-J12A8-K11A9-G12A10-H11A11-E12A12-F11A13-C12A14-D11A15-A12A16-B116 PAIR X 16 COLUMNDC RAF8 PAIR X 12 COLUMN ORTHO DIRECT RAMPIN MAPA1R12REFERENCE3.0REFERENCE87.4REFERENCE27.9THIS SHEET IS FOR96 DIFFERENTIAL PAIRS ONLYMATED DIMENSIONSDIVISIONAL SYMBOLSFUNCTIONAL SYMBOLS =0=0=CURRENT REV DESC: ADD 0.26MM COMPLIANT PARTNUMBERSEC NO:675528DRWN:JMENDOZA012021/06/15CHK'D:JMENDOZA012021/09/01APPR:JMENDOZA012021/09/01THIS DRAWING CONTAINS INFORMATION THAT IS PROPRIETARY TO MOLEX ELECTRONIC TECHNOLOGIES, LLC AND SHOULD NOT BE USED WITHOUT WRITTEN PERMISSION DIMENSION UNITS SCALE mm 2:1GENERAL TOLERANCES (UNLESS SPECIFIED)ANGULAR TOL±0.5 °4 PLACES±3 PLACES ±2 PLACES±0.13IMPULSE 8 PAIRORTHO DIRECT RAM - 90° - 2MM UNGUIDEDPRODUCT CUSTOMER DRAWINGDOCUMENT NUMBERDOC TYPE DOC PART REVISIONLLKKJJHHGGFFEED DCC BBORTHO PIN MAPPING(DC RAF - ORTHO DIRECT RAM)H1-T2H2-R1H3-P2H4-N1H5-M2H6-L1H7-K2H8-J1H9-H2H10-G1H11-F2H12-E1H13-D2H14-C1H15-B2H16-A1G1-R2G2-T1G3-N2G4-P1G5-L2G6-M1G7-J2G8-K1G9-G2G10-H1G11-E2G12-F1G13-C2G14-D1G15-A2G16-B1F1-T4F2-R3F3-P4F4-N3F5-M4F6-L3F7-K4F8-J3F9-H4F10-G3F11-F4F12-E3F13-D4F14-C3F15-B4F16-A3E1-R4E2-T3E3-N4E4-P3E5-L4E6-M3E7-J4E8-K3E9-G4E10-H3E11-E4E12-F3E13-C4E14-D3E15-A4E16-B3D1-T6D2-R5D3-P6D4-N5D5-M6D6-L5D7-K6D8-J5D9-H6D10-G5D11-F6D12-E5D13-D6D14-C5D15-B6D16-A5C1-R6C2-T5C3-N6C4-P5C5-L6C6-M5C7-J6C8-K5C9-G6C10-H5C11-E6C12-F5C13-C6C14-D5C15-A6C16-B5B1-T8B2-R7B3-P8B4-N7B5-M8B6-L7B7-K8B8-J7B9-H8B10-G7B11-F8B12-E7B13-D8B14-C7B15-B8B16-A7A1-R8A2-T7A3-N8A4-P7A5-L8A6-M7A7-J8A8-K7A9-G8A10-H7A11-E8A12-F7A13-C8A14-D7A15-A8A16-B7THIS SHEET IS FOR64 DIFFERENTIAL PAIRS ONLYMATED DIMENSIONSREFERENCE3.0REFERENCE19.9REFERENCE79.48 PAIR X 8 COLUMN ORTHO DIRECT RAMPIN MAPR8A14 PAIR X 16 COLUMNDC RAFDIVISIONAL SYMBOLSFUNCTIONAL SYMBOLS =0=0=CURRENT REV DESC: ADD 0.26MM COMPLIANT PARTNUMBERSEC NO:675528DRWN:JMENDOZA012021/06/15CHK'D:JMENDOZA012021/09/01APPR:JMENDOZA012021/09/01THIS DRAWING CONTAINS INFORMATION THAT IS PROPRIETARY TO MOLEX ELECTRONIC TECHNOLOGIES, LLC AND SHOULD NOT BE USED WITHOUT WRITTEN PERMISSION DIMENSION UNITS SCALE mm 2:1GENERAL TOLERANCES (UNLESS SPECIFIED)ANGULAR TOL±0.5 °4 PLACES±3 PLACES ±2 PLACES±0.13IMPULSE 8 PAIRORTHO DIRECT RAM - 90° - 2MM UNGUIDEDPRODUCT CUSTOMER DRAWINGDOCUMENT NUMBERDOC TYPE DOC PART REVISIONLLKKJ JHHGGFFEED DCC BB。
Paracrine Effects of Mesenchymal Stem Cells Induce Senescence and
Cell Transplantation , V ol. 24, pp. 631–644, 2015 0963-6897/15 $90.00 + .00Printed in the USA. All rights reserved. DOI: /10.3727/096368915X687787Copyright Ó 2015 Cognizant Comm. Corp. E-ISSN 1555-3892631Received January 27, 2015; final acceptance March 9, 2015. Online prepub date: March 24, 2015.Address correspondence to Dr. Helena Motaln, National Institute of Biology, Department of Genetic Toxicology and Cancer Biology, Ve na pot 111, SI-1000 Ljubljana, Slovenia. Tel: +386 5923 2870; E-mail: Helena.Motaln@nib.siParacrine Effects of Mesenchymal Stem Cells Induce Senescence andDifferentiation of Glioblastoma Stem-Like CellsKatja Kološa,* Helena Motaln,* Christel Herold-Mende,† Marjan Koršic ˇ,‡ and Tamara T. Lah*§*Department of Genetic Toxicology and Cancer Biology, National Institute of Biology, Ljubljana, Slovenia †Division of Neurosurgical Research, Department of Neurosurgery, University of Heidelberg, Heidelberg, Germany‡Department of Neurosurgery, University Medical Centre of Ljubljana, Ljubljana, Slovenia §Department of Chemistry and Biochemistry, Faculty of Chemistry and Chemical Technology,University of Ljubljana, Ljubljana, SloveniaGlioblastoma multiforme (GBM) displays high resistance to radiation and chemotherapy, due to the presence of a fraction of GBM stem-like cells (GSLCs), which are thus representing the target for GBM elimination. Since mesenchymal stem cells (MSCs) display high tumor tropism, we examined possible antitumor effects of the secreted factors from human MSCs on four GSLC lines (NCH421k, NCH644, NIB26, and NIB50). We found that conditioned media from bone marrow and umbilical cord-derived MSCs (MSC-CM) mediated cell cycle arrest of GSLCs by downregulating cyclin D1. PCR arrays revealed significantly deregulated expression of 13 genes asso-ciated with senescence in NCH421k cells exposed to MSC-CM. Among these, A TM, CD44, COL1A1, MORC3, NOX4, CDKN1A, IGFBP5, and SERPINE1 genes were upregulated, whereas IGFBP3, CDKN2A, CITED2, FN1, and PRKCD genes were found to be downregulated. Pathway analyses in GO and KEGG revealed their association with p53 signaling, which can trigger senescence via cell cycle inhibitors p21 or p16. For both, upreg-ulated expression was proven in all four GSLC lines exhibiting senescence after MSC-CM exposure. Moreover, MSC paracrine signals were shown to increase the sensitivity of NCH421k and NCH644 cells toward temozo-lomide, possibly by altering them toward more differentiated cell types, as evidenced by vimentin and GFAP upregulation, and Sox-2 and Notch-1 downregulation. Our findings support the notion that MSCs posses an intrin-sic ability to inhibit cell cycle and induce senescence and differentiation of GSLCs.Key words: Cell differentiation; Glioblastoma multiforme (GBM); Stem-like cancer cells; Mesenchymal stem cells (MSCs); Senescence; Therapy responseINTRODUCTIONGlioblastoma multiforme (GBM) is the most malignant type of brain tumor that is still incurable, with the overall survival of GBM patients being less than 15 months (35). This is due to highly infiltrative growth of GBM and its resistance to chemo/radiotherapies, preventing complete elimination of tumor cells, despite improvements made in surgical techniques and therapeutic protocols (7,45). The highly tumorigenic subpopulation of glioblastoma stem-like cells (GSLCs) present in the tumor and defined as a subpopulation of cells with stem-like properties, such as self-renewal, asymmetric division, and differentiation potential, essential for tumor development and propaga-tion in vivo (8,45) are presumably causative for GBM recurrence. Their variable abundance within the tumor mass has been confirmed in several studies (6,42,52). Their stemness and increased resistance to radiation andchemotherapy (4,16,28) are suggesting new therapeutic approaches focusing on GSLC targeting to improve the survival of GBM patients (35).Cell therapy is being revisited in view of using normal tissue stem cells, that is, human mesenchymal stem cells (MSCs) for cancer treatment. MSCs are multipotent stem cells most often isolated from birth-associated [i.e., umbili-cal cord (UC-MSC), cord blood (UCB-MSC)] and adult tissues, such as bone marrow (BM-MSC) and adipose tissue (A T-MSC) (31). They are supposed to have a wide therapeutic potential because of their immunomodulatory ability, wound- and neoplasma-directed homing, and tis-sue repair ability, relying on their differentiation potential (21,24). Already naive UCB-MSCs were shown to sup-press growth of various cancers, including GBM, as a 36% size reduction of C6 xenografts in mice was observed after UCB-MSC intratumoral injection (19). Likewise, a632 KOLOŠA ET AL.systemic A T-MSC coadministration with 8MGA glioma cells was shown to decrease de novo tumor development in mice by 63%, compared to control mice injected with 8MGA cells only (27). Injected A T-MSCs were shown to colocalize with CD133+ cells in GBM xenografts (2). Also in rat glioma models, the injection of UCB-MSCs and BM-MSCs resulted in tumor volume reduction (23), tumor growth inhibition (13), and increased survival of animals (33). Conditioned media (CM) derived from UC-MSCs and A T-MSCs were shown to inhibit the growth of U251 glioma cells (50), whereas BM-MSCs and their CM were shown to inhibit proliferation of U87MG, U251, and U373 glioma cells (31) and induce U87 cell senescence (32). In contrast, the UCB-MSCs, UC-MSCs, and A T-MSCs were shown to induce apoptosis in glioma cells (13,20,50). All of the above is implying the potential of MSCs for the development of novel anticancer cell-based therapies.As the intrinsic resistance of GSLCs to therapy pres-ents the main obstacle in GBM treatment, the repressive potential of MSCs on GSLCs (i.e., on their stemness poten-tial and viability) should be defined. To clarify the MSC effect on GSLCs, we utilized the established GSLC lines and primary culture of GBM cells enriched for GSLCs and exposed them to BM-MSC- and UC-MSC-derived CM. We speculated that the paracrine activity of the MSCs, namely their secreted factors, would cause the alteration of the GSLC behavior. Indeed, we observed both MSC-CMs to cause cell cycle arrest of GSLCs in the G 0/G 1 phase and to induce their senescence. To our knowledge, we are also first to demonstrate that the exposure of GSLCs to MSC paracrine signals changes the gene expression of GSLCs, which is driving them toward a more differentiated phe-notype. The MSC-CM also increased the sensitivity of GSLCs to chemotherapeutic temozolomide (TMZ), com-monly used in GBM treatment.MATERIALS AND METHODSCell CulturesGSLC lines NCH421k (male, age 66) and NCH644 (male, age 66) were isolated from GBM resection and checked for stemness potential, as described previously (8,10).NIB26 (female, age 75) and NIB50 (male, age 81) cells were isolated from fresh GBM samples obtained from the University Medical Centre of Ljubljana (study approved by the National Ethics Committee). Upon mechanical and enzymatic dissociation, the isolated cells were grown as spheroids (with a presumed enriched fraction of GSLCs) under the same conditions as NCH cells in neurobasal (NBE) medium (Gibco, Life Tech. Corp., Paisley, UK) sup-plemented with 2 mM l -glutamine (PAA Lab, Pasching, Austria), 100 U penicillin, 1,000 U streptomycin (PAA Lab), 1× B-27 (Gibco), 1 U/ml heparin (Sigma-Aldrich, Steinheim, Germany), 20 ng/ml EGF in 20 ng/ml bFGF(Gibco). Both derived cell lines were tested for the CD133 expression by flow cytometry.The U87 cell line was grown as described previously (31).Human MSCsHuman BM-MSCs were purchased from Lonza Bio-science (Walkersville, MD, USA) (BM-MSC2: male, age 19, 6F4393; BM-MSC3: female, 22, 7F3677) and cultured according to the manufacturer’s recommendations. Briefly, MSCs were grown in Dulbecco’s medium (DMEM; 5921; Sigma-Aldrich) with 10% fetal bovine serum (FBS; PAA Lab), supplemented with 100 U penicillin (PAA Lab), 1,000 U streptomycin (PAA Lab), 2 mM l -glutamine (PAA Lab), Na-pyruvate (Gibco), and nonessential amino acids (Sigma-Aldrich).UC-MSCs were isolated from Wharton’s jelly accord-ing to standard protocol (47) in the study approved by the National Ethics Committee, Doc. No. 134/01/11. Umbilical cords were collected at cesarean section (37–41 weeks) upon obtained informed consent, and isolated UC-MSCs were cultured the same as BM-MSCs. UC-MSCs were characterized for CD13+, CD29+, CD44+, CD73+, CD90+, CD105+, CD14−, CD34−, CD45−, HLA-DR − surface marker expression, and osteogenic, chondrogenic, and adipogenic differentiation as recommended (15). Two UC-MSC clones (UC-MSC-10, female and UC-MSC-13, male) with the highest proliferation potential and homogenous spindle-like morphology were used in further experiments.Conditioned Media (CM) CollectionTwo different clones of BM-MSCs and the two clones of UC-MSCs were plated into T75 flasks (Corning, Cambridge, MA, USA) at a density of 6,000 cells/cm 2, and a day before the cells would have reached 90% confluence, the standard MSC cultivation medium, described above, was changed to 12.5 ml NBE medium (used in GSLC cul-ture). After 24 h, this CM was collected and pooled from both BM-MSC lines or from both UC-MSC cell lines, cen-trifuged at 300 × g for 10 min at 4°C, and stored at −80°C. Prior to the experiment, the CM were mixed in a 1:1 ratio with fresh NBE medium and designated as BM-CM and UC-CM, respectively. Similarly, the NBE CM were col-lected after 24 h from cultured NCH421k and NCH644 spheroids and diluted at a 1:1 ratio with fresh NBE medium and when used designated as NBE-CM.Surface Marker Detection by Flow CytometryUC-MSCs (106) were harvested, washed with 1× phosphate-buffered saline (PBS; Gibco), and incubated with antibodies against CD13 (BD#557454), CD29 (BD# 559883), C D 44 (BD #555479), C D 73 (BD #550257), C D 90 (BD#555596), CD14 (BD#555397), CD34 (BD#555821), CD 45 (BD #555482), HLA-D R (BD #340688) (all fromMSCs INDUCE SENESCENCE AND DIFFERENTIATION OF GSLCs 633BD Biosciences, San Jose, CA, USA), CD105 (MHCD1 0505; Molecular Probes, Eugene, OR, USA), and isotype controls [FITC-IgG1 (BD#555748), FITC-IgG2a (BD#55 5573), PE-IgG1 (BD#555749), PE-IgG2b (BD#555743), APC-IgG1 (BD#555751); all from BD Biosciences] as instructed by the manufacturer. All cells were addition-ally stained with propidium iodide (PI; BD Biosciences) to exclude dead cells, washed, and analyzed by flow cytome-try using a BD FACSCalibur TM and the CellQuest Software (both BD Biosciences).NIB26 and NIB50 cells were tested for the expres-sion of CD133 protein using the antibodies recognizing AC133 or CD133/2 (AC141) epitope by the protocol described below. The staining results proved to overlap properly (8).NCH421k and NCH644 (106) cells were harvested upon 72-h exposure to NBE, NBE-CM, UC-CM, and BM-CM, washed with 1× PBS, and incubated with 10 µl CD133/2-PE antibody (130-090-853, Miltenyi Biotec, San Diego, CA, USA) or 20 µl isotype control antibody (PE-IgG2b, BD Biosciences) in equal concentrations. All cells were PI (BD Biosciences) stained for live cell analysis (20 min at 4°C), washed, and analyzed by flow cytometry using a BD FACSCalibur TM and the CellQuest Software (both BD Biosciences).Differentiation of UC-MSCsAdipogenic Differentiation. The adipogenic differen-tiation medium was composed of DMEM supplemented with 10% FBS, 1 µM dexamethasone (Sigma-Aldrich), 0.5 mM 3-isobutyl-1-methyl-xanthine (IBMX; Sigma-Aldrich), 10 µg/ml insulin (Sigma-Aldrich), 100 µM indo-methacin (Sigma-Aldrich). After 72 h, cells were medium changed to adipogenic maintenance medium: D MEM supplemented with 10% FBS and 10 µg/ml insulin for 24 h, and this sequence was repeated three times, fol-lowed by cultivation for 1 week in maintenance medium. After 21 days, cells were fixed in 4% paraformaldehyde for 10 min, and accumulated lipid-rich vacuoles were stained with 0.3% Oil red O (Sigma-Aldrich) for 30 min.O steogenic Differentiation. The osteogenic differen-tiation medium was prepared with D MEM containing 10% FBS, 1 µM dexamethasone (Sigma-Aldrich), 50 µg/ ml l-ascorbic acid (Sigma-Aldrich), and 10 mM glyc-erophosphate (Sigma-Aldrich). After 21 days, cells were fixed in 4% paraformaldehyde for 10 min and stained with 1% Alizarin red S (Sigma-Aldrich) for 30 min to detect calcified extracellular matrix.Chondrogenic Differentiation. Cells were exposed to chondrogenic differentiation medium composed of DMEM, 10% FBS, 10 ng/ml TGF-b3 (Sigma-Aldrich), and 50 µg/ml l-ascorbic acid for 21 days. To detect matrix deposition of sulfated glycosaminogycans (GAGs), cells were fixed in 4% paraformaldehyde for 10 min and stained with 1% Alcian blue 8-GX (Sigma-Aldrich) in 3% acetic acid (Sigma-Aldrich).Cell Cycle AnalysisNCH421k cells were exposed to NBE, UC-CM, and BM-CM for 72 h, trypsinized [0.25% Trypsin-ED TA (Gibco)], washed with 1× PBS, and left in 75% etha-nol overnight at 4°C. Upon washing with 1× PBS, cells were incubated in 0.5 ml PI/RNase staining buffer (BD Pharmingen, San D iego, CA, USA) for 15 min at RT. Cell cycle analysis was performed using the WinMDI and Cyclored on the BD FACSCalibur TM with the CellQuest Software.Apoptosis AssayNCH421k cells were exposed to NBE, UC-CM, and BM-CM for 72 h, harvested, and early/late apoptotic cells detected by staining with Annexin-V-FITC and PI (BD Pharmingen) according to the manufacturer’s protocol. Stained cells were analyzed using BD FACSCalibur TM and the CellQuest Software. Staurosporine (STS; Sigma-Aldrich) treatment (2 µM, 6 h) served as a positive control for apoptosis.Mitochondrial Membrane Potential (MMP) Detection MMP change in NCH421k cells grown in NBE, UC-CM, and BM-CM for 72 h was measured with the Mitochondrial Permeability Transition Detection Kit JC-1 (ImmunoChemistry Technologies, Bloomington, MN, USA) according to the manufacturer’s instructions. STS (2 µM, 6 h) and carbonylcyanide-chlorophenyl-hydrazone (CCCP; 25 µM, 30 min; ImmunoChemistry Technologies)-treated NCH421k cells were used as a positive control. JC-1 staining was analyzed using a Synergy™ MX Microplate Reader (Bio-Tec Instruments, Winooski, VT, USA). The change of MMP was assessed by the ratio of red versus green fluorescence readings.Cell SenescenceNCH421k, NCH644, NIB26, and NIB50 spheroids were grown in NBE, UC-CM, and BM-CM for 72 h; GSLC spheroids were dissociated to single cells and fixed in 0.5% glutaraldehyde solution (Sigma-Aldrich) for 20 min at RT. The proportion of cells positive for senescence indicative of b-galactosidase (SA-b-Gal) activity was evaluated as described previously (14). Quantitative Real-Time PCR (qRT-PCR) andPCR ArraysTotal RNA was isolated from three biological replicates of the U87 cell line and NCH421k, NCH644, NIB26, and NIB50 cells grown in NBE, UC-CM, and BM-CM for 72 h using Trizol TM reagent (Invitrogen Limited,634 KOLOŠA ET AL.Paisley, UK) following the manufacturer’s instructions. Total RNA (1 µg) was used for cDNA generation using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA). Gene expression of cyclin-dependent kinase inhibitor 2A (CD KN2A; p16), cyclin-dependent kinase inhibitor 1A (CD KN1A; p21), cyclin D 1 (CCND 1), BCL-2-associated X protein (BAX), B-cell CLL/lymphoma 2 (BCL-2), prominin 1 (PROM1; CD133), SRY (sex-determining region Y)-box 2 (Sox-2), nestin (NES), Notch-1, tubulin b 3 class III (TUBB3), vimentin (VIM), and glial fibrillary acidic protein (GFAP) was quantified by real-time quantita-tive PCR on ABI 7900 HT Sequence D etection System (Applied Biosystems). Real-time PCR reactions com-prised of cDNA added to TaqMan Universal PCR Master Mix and TaqMan Gene Expression Assays (all Applied Biosystems): CDKN2A, Hs00923894_m1; CDKN1A, Hs 00355782_m1; CCND1, Hs00765553_m1; BAX, Hs999 99001_m1; BCL-2, Hs0060823_m1; PROM1, Hs010092 50; Sox-2, Hs01053049_s1; NES, Hs00707120_s1; Notch-1, Hs01062014_m1; TUBB3, Hs00801390_s1; VIM, Hs001 85584_m1; GFAP, Hs00157674_m1; p53, Hs01034249; and glyceraldehyde 3-phosphate dehydrogenase (GADPH) TaqMan probe (Assay No. 4310884E) as an internal con-trol. The analyses were performed with SDS v2.2 soft-ware (Applied Biosystems) and comparative Ct method (DD Ct algorithm).By using generated cD NA from NCH421k exposed to control and both types of MSC-CM, the expression of 84 senescence-related genes was determined also fol-lowing the Cellular Senescence RT-Prolifer PCR Array (Qiagen-SABiosciences, Hilden, Germany) protocol. On the Cellular Senescence RT-Prolifer PCR Array, the aver-age of three different housekeeping genes, ACTB (actin b ), GAPDH, and ribosomal protein L13a (RLP13A), was used in calculations as an internal control. Fold increase in mRNA levels was calculated with the DD Ct method by the provided online program available at http://pcr /pcr/arrayanalysis.php. Results are expressed as means of three independent experiments done in duplicate.Cytotoxicity Testing After Exposure to Temozolomide and 5-Fluoro-Uracil by MTS Cell Viability AssayNCH421k and NCH644 cells were plated into 96-well plates (Corning) at a density of 104 cells/well and left to grow for 24 h before adding NBE, UC-CM, BM-CM, and TMZ (2.5–1000 µM) or 5-fluoro-uracil (5-FU) (0.01–150 µM) (both Sigma-Aldrich). A fter 48 h and 72 h of TMZ and 5-FU treatment, the CellTiter 96® AQ ueous MTS reagent [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium; Promega Corporation, Madison, WI, USA; Sigma-Aldrich], was added to eachwell to make 333 m g/ml final concentration. Three hours later, the absorbance at 490 nm (reference 690 nm) was measured with the Synergy™ MX Microplate Reader (Bio-Tec Instruments).Statistical AnalysesAll the above experiments were performed in duplicate and independently repeated three times. To test the effect of MSC-CM on GSLCs compared to control medium, the ANOV A test with post hoc D unnett’s test was per-formed using GraphPad Prism version 5.01 for Windows (GraphPad Software, La Jolla, CA, USA). A value of p < 0.05 was considered significant. Data are expressed as the mean ± standard deviation (SD).RESULTSUmbilical Cord-Derived MSC Lines (UC-MSCs) and Primary GSLCs Were Successfully GeneratedWe established six MSC lines from umbilical cords (UC-MSCs) obtained from healthy donors. These UC- MSCs exhibited plastic adherence in culture and typical spindle-shaped morphology. We confirmed the presence of CD 13, CD 29, CD 44, CD 73, CD 90, and CD 105 and the absence of CD14, CD34, CD45, and HLA-DR sur-face antigens in all UC-MSC clones by flow cytometry and verified their osteo-, chondro-, and adipogenic differ-entiation potential (data available, not shown). This char-acterization is in accordance with suggested criteria for MSC phenotype confirmation (15); thus, we further used two UC-MSC clones together with two BM-MSC lines for UC-CM and BM-CM production.Purified NCH421k and NCH644 GSLC lines were established previously by Campos et al. (10) and con-firmed by DNA copy-number profiling and CGH array to show typical GBM chromosome aberrations (8,46) and to highly express CD133 epitope 1 antigen detected by AC133 antibody (9). Flow cytometric analysis also con-firmed expression of CD133 in NIB26 (79.9% CD133+ cells) and NIB50 (71.4% CD 133+ cells) that were iso-lated from primary GBM tissue and grown in spheroids to enrich for their GSLCs fraction (Fig. 1A). Analysis of p53 status in NCH421k, NCH644, NIB26, and NIB50 cells was determined by qRT-PCR with U87 cell line (wild-type p53) serving as reference (54).MSC-CM Caused G 0 /G 1 Phase Cell Cycle Arrest via Cyclin D1 DownregulationCell cycle analysis of NCH421k cells grown with NBE or either type of CM for 72 h was performed. It revealed an increased number of NCH421k cells halted in G 0/G 1 phase in the presence of UC-CM, compared to NBE (Fig. 2A). Additionally, the G 0/G 1 cell cycle arrest of NCH421k cells, grown in UC-CM and BM-CM, was confirmed byMSCs INDUCE SENESCENCE AND DIFFERENTIATION OF GSLCs 635Figure 1. GSLCs isolated from primary GBM tissue express high levels of CD133. NIB26 and NIB50 cells were isolated from primary GBM tissue and grown in spheroids to enrich the GSLC fraction. Flow cytometry analysis of CD133 expression showed (A) 79.9% CD133+ cells in NIB26 and (B) 71.4% CD133+ cells in NIB50 cell culture. (C) Analysis of p53 gene expression in U87, NCH421k, NCH644, NIB26, and NIB50 cells by qRT-PCR.Figure 2. MSC-CM interferes with the cell cycle of GSLCs. (A)Cell cycle analysis of NCH421k cells performed by flow cytometry after 72-h treatment with both types of MSC-CM. (B) Cyclin D1 (CCND1) expression (qRT-PCR) was decreased in all GSLC lines after 72-h treatment with UC-CM and BM-CM. The mean ± SD of three independent experiments are provided. *p < 0.05, **p < 0.01, ***p < 0.001.Morphology of native cells: (C) Control NCH421k cells with compact spheroid morphology; (D) NCH421k spheroid morphology changed upon UC-CM treatment for 72 h; and (E) NCH421k spheroid morphology changed in the presence of BM-CM after 72 h. (F)Control NCH644 cells with compact spheroid morphology; (G) UC-CM treatment for 72 h changed NCH644 spheroid morphology to adherent type of growth; and (H) BM-CM presence after 72 h changed NCH644 spheroid morphology to adherent type of growth. Scale bar: 100 µm.636 KOLOŠA ET AL.CCND1 gene expression analysis. This showed CCND1 gene expression to decrease 4.2-fold in UC-CM and 2.9-fold in BM-CM (Fig. 2B). Similarly, the CCND1 gene expression was found downregulated in NCH644, NIB26, and NIB50 cells exposed to CM. The above was associated with the indicative size reduction and loosened structure of NCH421k and NCH644 spheroids grown in both types of MSC-CM (Fig. 2C–E). The BM-CM was even shown to induce the adherent growth of NCH644 cells (Fig. 2F–H). Altogether, our data imply the capacity of MCS paracrine factors to cause the cell cycle arrest of GSLCs through decrease in the cyclin D1 expression level.MSC-CM Affected Mitochondrial Membrane Potential but Did Not Induce Apoptosis in GSLCsThe induction of apoptosis in NCH421k cells grown in MSC-CM of both origins for 72 h was evaluated by MMP assay. A significant decrease in MMP was detected in NCH421k cells when exposed to UC-CM (23%) and BM-CM (31%) (Fig. 3A). Since MMP change is not solely indicative of apoptosis, BAX and BCL-2 gene expression was analyzed in the NCH421k, NCH644, NIB26, and NIB50 cells cultured in MSC-CM. The analysis of the BAX/BCL-2 gene ratio failed to demonstrate any signifi-cant change to occur in the CM-cultured GSLCs, com-pared to control (NBE) ones (Fig. 3B). To further exclude the onset of apoptosis, the annexin V staining was per-formed. This also failed to detect increased apoptosis in NCH421k cells cultured in MSC-CM versus control cells with staurosporin treatment used as positive control (Table 1). Altogether our results showed that by addition of MSC-CM containing MSC paracrine factors, the apop-tosis in GSLCs cannot be induced.MSC-CM-Activated Senescence in GSLCsAs MSC-CM influenced the cell cycle of GSLCs, but did not cause apoptosis, the senescence in exposed GSLCs was investigated by using the indicative b-galactosidase staining. A significantly increased number of senescent cells was observed in NCH421k (17% and 33%), NCH644 (33% and 39%), NIB26 (20% and 25%), and NIB50 (30% and 31%) cells exposed to UC-CM and BM-CM, respec-tively, for 72 h, when compared to control NCH421k (Fig. 4A). In GSLCs exposed only to NBE, the percent-age of senescent cells was 10.7% in NCH421k, 6.6% in NCH644, 3.3% in NIB26, and 8.2% in NIB50 cells.To get insight into the senescence signaling, NCH421k cells were subjected to both types of MSC-CM, and qRT-PCR analysis of 84 genes associated with senescence was conducted by the human Senescence RT2 Profiler PCR Array (Table 2). The PCR arrays revealed changed expression of 13 genes involved in senescence (ATM, CDKN1A, CDKN2A), cell adhesion (CD44, COL1A1), oxidative stress (PRKCD, NOX4), IGF-related path-way (IGFBP3, IGFBP5), p53/pRb signaling (MORC3, CITED2), and cytoskeleton formation (FN1, SERPINE1) (Table 2). Those genes were consistently up- and down-regulated in exposed NCH421k cells at least 2.1-fold. In addition, KEGG pathway analysis identified the involve-ment of five genes, SERPINE1, CD KN1A, IGFBP3, CD KN2A and ATM, in the p53 pathway (Table 3) and induced in senescence.The PCR Array results were validated with qRT-PCR on independent mRNA samples of GSLCs, which confirmed increased expression of the CDKN1A gene in NCH421k cells grown for 72 h in UC-CM (1.4-fold) and BM-CM (6.7-fold). Likewise, the CD KN1A expression increasedFigure 3. MSC-CM does not induce apoptosis in GSLC lines. (A) Mitochondrial membrane potential (MMP) loss was ascertained by JC-1 staining after exposure of NCH421k cells to UC-CM and BM-CM for 72 h. (B) QRT-PCR analysis of Bax and Bcl-2 gene expression in NCH421k, NCH644, NIB26, and NIB50 cells after exposure to both types of MSC-CM for 72 h. The ratio between the Bax and Bcl-2 was unaffected. Data shown are mean ± SD of three independent experiments. ***p < 0.001. CCCP, carbonylcyanide-chlorophenyl-hydrazone; STS, staurosporin.MSCs INDUCE SENESCENCE AND DIFFERENTIATION OF GSLCs 637Table 1. Annexin V/PI Staining of NCH421k Cells Exposed to Staurosporin (STS) and MSC-CMLive Early Apoptosis Late Apoptosis DeadNCH421k 93.34 ± 4.20 4.83 ± 2.63 1.06 ± 1.700.76 ± 0.69NCH421k + STS 42.98 ± 12.73*45.26 ± 13.87* 5.66 ± 0.560.80 ± 1.02NCH421k + UC-CM 91.06 ± 2.547.01 ± 2.33 1.24 ± 1.410.56 ± 0.55NCH421k + BM-CM 88.82 ± 6.517.69 ± 2.45 2.82 ± 4.320.67 ± 0.68The NCH421k cells were exposed to UC-CM and BM-CM, and the apoptotic cells were determined by flowcytometry as described in Materials and Methods. The exposure to STS, inducing intrinsic apoptotic pathway,was used as positive control. The data of three independent experiments are expressed as the mean ± standarddeviation (SD) and *p< 0.05 was considered significant.Figure 4. MSC-CM activates senescence in all GSLC lines. (A) Senescence-associated b-galactosidase (SA-b-Gal) staining revealed activation of senescence in NCH421k, NCH644, NIB26, and NIB50 cells after exposure to UC-CM and BM-CM upon 72-h incuba-tion. qRT-PCR analysis of (B) p21 expression and (C) p16 expression after 72-h treatment with both types of MSC-CM in all GSLCs. Data shown are mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001. (D) SA-b-Gal staining of dissociated and fixed NCH421k, NCH644, NIB26, and NIB50 cells after UC-CM and BM-CM presence for 72 h. Scale bar: 20 µm.638 KOLOŠA ET AL.in NCH644 (3.2-fold) and NIB50 cells (5.0-fold) exposed to BM-CM (Fig. 4B). The expression of the CD KN2A gene also increased in NCH644 (3.4-fold), NIB26 (7.7-fold), and NIB50 (1.9-fold) exposed to BM-CM (Fig. 4C), whereas it decreased 1.7-fold in NCH421k cells exposed to BM-CM (Fig. 4C). The above was consistent with the observed b-galactosidase staining (Fig. 4D), altogether implying the ability of the MSC paracrine factors to induce GSLC growth arrest and senescence.MSC-CM Affected the Expression of GSLC Markers To evaluate the impact of MSC-CM on the stemness of GSLCs, the expression of indicative GSLC marker CD133/prominin was examined by qRT-PCR (using gene expression assay recognizing all seven splice variants of the prominin transcript). An increased CD133 gene expression was observed in NCH421k cells (1.8-fold and 2.5-fold) and NIB26 cells (3.0-fold), whereas in NCH644 cells a decreased CD133 gene expression (2.3-fold and 3.9-fold) was noticed after 72 h of exposure to UC-CM and BM-CM, respectively (Fig. 5A). Flow cytometry analysis of CD133 glycosylated epitope (CD133/2) revealed a decrease in NCH644 cells exposed to UC-CM (59.5%) and to BM-CM (61.3%) for 72 h (Fig. 5B). In NCH421k cells exposed to both types of MSC-CM, flow cytometry analysis failed to demonstrate changes in CD133 antigen expression. Also, a prolonged culture of NCH421k cells in MSC-CM for 7 days had no impact on their CD133 antigen expression (Fig. 5B).Beside CD133 expression, we also investigated the MSC-CM impact on the expression of other markers asso-ciated with stemness of GSLCs, such as Sox-2, Nestin, and Notch-1. The qRT-PCR analyses revealed decreased expres-sion of Sox-2 in NCH421k (1.5-fold, 1.8-fold), NCH644 (2.0-fold, 1.1-fold), NIB26 (6.3-fold, 4.3-fold), and NIB50 cells (5.1-fold, 5.2-fold) exposed to UC-CM and BM-CM, respectively (Fig. 5C). Likewise, a decreased expression of Notch-1 was noted in NCH644 (1.7-fold, 1.1-fold), NIB26 (3.9-fold, 2.9-fold), and NIB50 cells (2.3-fold, 1.7-fold) when exposed to UC-CM and BM-CM, respectively, yet with no change observed NCH421k cells exposed to either type of MSC-CM (Fig. 5D). Also Nestin expression didTable 2. Differentially Expressed Genes in MSC-CM-Cultured NCH421k CellsNCH421k + UC-CM NCH421k + BM-CMGene symbol Fc p Value Fc p Value Pathway/Process ATM 2.60.032*−1.20.406Senescence CD44 2.70.022* 1.40.241Cell adhesion COL1A17.50.043* 1.50.210Cell adhesion MORC3 2.10.046*−1.10.606p53/pRb signaling NOX4 2.10.045* 1.80.163Oxidative stress CDKN1A 3.00.054* 6.90.001*Senescence IGFBP5 1.50.34330.10.017*IGF related SERPINE1 4.10.172 3.80.010*Cytoskeleton IGFBP3−2.10.009* 2.00.048*IGF related CDKN2A 1.30.311−2.10.022*Senescence CITED2−1.40.101−2.80.034*p53/pRb signaling FN1 2.10.082−2.40.012*Cytoskeleton PRKCD−1.40.294–2.20.016*Oxidative stress Listed are deregulated genes in NCH421k cells exposed to either UC-CM or BM-CM relative to NBE exposed NCH421k cells. Fold increase in mRNA levels are calculated as described in Materials and Methods. The mean ± SD of three independent experiments are provided. *p< 0.05. Fc, fold change.Table 3. Identification of Genes Involved in p53 Pathway With KEGG Pathway AnalysisKEGG Term p Value p FDR Biomolecule IDs Gene Namep53 signaling pathway11.73E-12 434.33E-12 ENSG00000106366ENSG00000124762ENSG00000146674ENSG00000147889ENSG00000149311 SERPINE1 CDKN1A IGFBP3 CDKN2A ATMDifferentially expressed genes in NCH421k cells exposed to both types MSC-CM (Table 2) were subjected to ontology enrichment analysis in KEGG to detect their involvement in cellular pathways. Biomolecular identifica-tion number (Biomolecule IDs) corresponds to ENSEMBL gene description (); p value (<0.01); p FDR (false discovery rate, <0.01).。
Testo 174 数据记录仪 使用手册说明书
testo 174 · Datenlogger Bedienungsanleitungtesto 174 · Data loggersInstruction manualtesto 174 · Enregistreur de données Mode d'emploitesto 174 · Data loggerManual de instruccionestesto 174 · Data loggerIstruzioni per l'usotesto 174 · Data loggerManual de instruçõesDeutsch (3)English (19)Français (38)Español (49)Italiano (65)Português (81)21 Contents1Contents1Contents (19)2Safety and the environment (20)2.1About this document (20)2.2Ensure safety (21)2.3Protecting the environment (21)3Specifications (21)3.1Use (21)3.2Technical data (22)4First steps (24)4.1Releasing/securing data logger (24)4.2Inserting batteries (25)4.3Connecting data logger to PC (26)5Display and control elements (27)5.1Display (27)5.2Key functions (28)6Using the product (29)6.1Programming data logger (29)6.2Mounting wall bracket (29)6.3Reading out data (30)7Maintaining the product (30)7.1Changing the batteries (30)7.2Cleaning the instrument (31)8Tips and assistance (32)8.1Questions and answers (32)8.2Accessories and spare parts (32)192 Safety and the environment202 Safety and the environment2.1About this documentUse> Please read this documentation through carefully andfamiliarize yourself with the product before putting it to use. Payparticular attention to the safety instructions and warning advicein order to prevent injuries and damage to the products.> Keep this document to hand so that you can refer to it whennecessary.> Hand this documentation on to any subsequent users of theproduct. Symbols and writing standardsRepresentation ExplanationWarning advice, risk level according to thesignal word:Warning! Serious physical injury may occur.Caution! Slight physical injury or damage tothe equipment may occur.> Implement the specified precautionarymeasures.Note: Basic or further information. 1. ...2. ...Action: more steps, the sequence must be followed. > ...Action: a step or an optional step. - ...Result of an action. MenuElements of the instrument, the instrument display or the program interface. [OK]Control keys of the instrument or buttons of the program interface. ... | ... Functions/paths within a menu.“...” Example entries3 Specifications2.2Ensure safety> Only operate the product properly, for its intended purpose and within the parameters specified in the technical data. Do notuse any force.> Never use the instrument to measure on or near live parts.> Keep the read-out contacts on the rear of the data logger inparticular away from live parts!> Carry out only the maintenance and repair work on thisinstrument that is described in the documentation. Follow theprescribed steps exactly. Use only original spare parts fromTesto.2.3Protecting the environment> Dispose of faulty rechargeable batteries/spent batteries inaccordance with the valid legal specifications.> At the end of its useful life, send the product to the separatecollection for electric and electronic devices (observe localregulations) or return the product to Testo for disposal.3Specifications3.1UseThe testo 174 data loggers are used for storing and reading outindividual readings and series of measurements.Readings are measured and stored with the testo 174 andtransmitted to the PC via the interface, where they can be read outand evaluated using the testo ComSoft software. The data loggercan also be programmed individually via the software.Examples of applicationstesto 174T is ideally suited for temperature measurement in inrefrigerators, freezers, cold rooms and cold shelves.testo 174H monitors the climatic conditions, e.g. in warehouses,offices and in the manufacturing sector.213 Specifications223.2 Technical datatesto 174TCharacteristic Values Probe typeInternal NTC temperature sensor Measuring range-30 to +70 °C Accuracy± 0.5 °C (-30 to +70 °C) Resolution 0.1 °C Operatingtemperature-30 to +70 °C Storagetemperature-40 to +70 °C Battery type2 x3 V button cell (2 x CR 2032 lithium) Life500 days (15 min measuring cycle, +25 °C) Protection classIP65 Meas. cycle1 min - 24 h (can be selected) Memory 16,000 readingsSoftwarecan be used with Windows XP, Vista, Win7 Standards2004/108/EC, complies with the directives in accordance with the standard EN 128302 Warranty24 months, warranty conditions: see website /warranty2 Please note that with this instrument in accordance with EN 12830 a regular inspection and calibration as per EN 13486 must be performed(recommendation: annually). Contact us for more information.3 Specifications23 testo 174HCharacteristicValues Probe typeNTC temperature sensor and internal capacitive humidity sensor Measuring range 0 to 100 % RH (non-dewing atmosphere),-20 to +70 °CHumidity accuracy ± 3 % RH (2 % RH to 98 % RH)± 1 digit +0.03 % RH/K Temperatureaccuracy± 0.5 °C (-20 to +70 °C) Resolution0.1 % RH, 0.1 °C Operatingtemperature-20 to +70 °C Storagetemperature-40 to +70 °C Battery type2 x3 V button cell (2 x CR 2032 lithium) Life1 year (15 min measuring cycle, +25 °C) Protection classIP20 Meas. cycle1 min - 24 h (can be selected) Memory2 x 8000 readings Softwarecan be used with Windows XP, Vista, Win7 Warranty24 months, warranty conditions: see website /warranty EC Directive 2004/108/EC4 First steps244 First steps4.1Releasing/securing data logger The data logger is delivered secured.Releasing data logger1. Push both locks on the bottom of the wall bracket outwards.2. Slide data logger out of the wall bracket.Securing data logger1. Slide data logger into the wall bracket.2. Push both locks on the bottom of the wall bracket inwards.4 First steps254.2 Inserting batteries1.Place the data logger on its front.2 Open battery compartment cover on the rear of the data loggerby turning to the left and remove it.Use a coin for this.3. Insert supplied batteries (3 V button cell, CR 2032 lithium) sothat the + pole is always visible.4.Place battery compartment cover on the data logger and close by turning to the right.Use a coin for this.- The current temperature is shown on the display screen.4 First steps26 4.3 Connecting data logger to PC1. Install testo ComSoft software.The software is available in the Internet as a free download requiring registration: , International ,Service&Support | Download Center . The instructions for the installation and operation of thesoftware can be found in the testo ComSoft instruction manual that is downloaded together with the software.The software can be ordered on CD (article no. 0572 0580) if the download from the Internet is not desired. 2. Connect the connecting cable of the interface to a free USBinterface on the PC.3. Slide the data logger into the bracket of the interface.4. Configure data logger, see separate testo ComSoft instructionmanual.5 Display and control elements275Display and control elements 5.1 DisplayDepending on the operating status, various information may be shown in the display. A detailed depiction of the information that can be called up can be found in the quickinstruction manual that is included with every data logger. 1 Measurement program running2 Measurement program over3 Wait for start of measurement program4 Highest saved reading5 Lowest saved reading6 Reading7 Units8 Start criterion Date/time programmed9 Battery capacity5 Display and control elements28Icon Capacity75-100%50-75%25-50%10-25%<10%Battery empty (measurement program wasstopped).> Read out data and change battery (seeChanging the batteries page 30 ).10Lower alarm value:• Flashes: programmed alarm value is shown• Lights up: programmed alarm value was undershot11Upper alarm value• Flashes: programmed alarm value is shown• Lights up: programmed alarm value was exceeded The display speed of liquid crystal displays slows down attemperatures below 0 °C (approx. 2 seconds at -10 °C,approx. 6 seconds at -20 °C) for technical reasons. Thisdoes not influence the measuring accuracy.5.2Key functions✓Operating status Wait and start criterion Button startprogrammed.> P ress and hold GO button approx. 3 seconds to start the measurement program.- The measurement program starts and Rec appears in the display.✓Operating status Wait:6 Using the product29> Press GO button in order to change between displays of theupper alarm value, lower alarm value, battery life and last reading.- These are shown in the specified sequence in the display. ✓ Operating status Rec or End :> Press GO button in order to change between displays of thehighest saved reading, lowest saved reading, upper alarm value, lower alarm value, battery life and last reading.- These are shown in the specified sequence in the display.6 Using the product6.1Programming data loggerIn order to adapt the programming of your data logger to yourindividual requirements, you require the testo ComSoft software. It is available in the Internet as a free download requiring registration , International , Service&Support | DownloadCenter .The instructions for the installation and operation of the software can be found in the testo ComSoft instruction manual that is downloaded together with the software.6.2 Mounting wall bracket Mounting materials (e.g. screws, anchor plugs) are not included in the delivery.✓ Data logger is removed from the wall bracket (see Releasingdata logger page 24).1. Position wall bracket at the desired location.2. Using a pen or similar, mark the location for the fasteningscrew.3. Prepare the fastening location for the attachment in accordancewith the material (e.g. drill hole, insert anchor plug).4. Fasten wall bracket using an appropriate screw.7 Maintaining the product306.3 Reading out dataThe data logger is read out and the read-out data are processed further by means of the testo ComSoft software, see separate instruction manual.7 Maintaining the product7.1Changing the batteries The running measurement program is stopped when the battery is changed. The stored data are preserved,however. 1. R ead out stored data, see testo ComSoft software instructionmanual.✓ I f it is no longer possible to read out the stored data becausethe battery capacity is too low:> Change batteries and then read out the stored data.2.Place the data logger on its front. 3. Open battery cover on the rear of the data logger by turning tothe left. Use a coin for this.4. Remove empty batteries from the battery compartment.5. Insert two new batteries (3 V button cells, CR 2032 lithium) intothe instrument so that the + pole is always visible.7 Maintaining the product 31Only use new branded batteries. If a partially used battery is inserted, the calculation of the battery capacity is not performed correctly. 6. Place battery compartment cover on the battery compartmentand close by turning to the right. Use a coin for this. - The current reading is shown on the display. The data logger must be reconfigured. To do so, the testo ComSoft software must be installed on the computer and a connection to the data logger must be set up (seeConnecting data logger to PC page 26).7. Slide the data logger into the bracket of the interface.8. Start testo ComSoft software and create a connection to thedata logger.9. Reconfigure data logger or install the old stored configuration,see separate testo ComSoft instruction manual.- The data logger is once again ready for use.7.2 Cleaning the instrument CAUTIONDamage to the sensor!> Ensure that no liquid enters the inside of the housing.> I f the housing of the instrument is dirty, clean it with a dampcloth.Do not use any aggressive cleaning agents or solvents! Weak household cleaning agents or soap suds can be used.8 Tips and assistance328 Tips and assistance8.1Questions and answersIf you have any questions, please contact your dealer or Testo Customer Service. The contact details can be found on the back of this document or on the Internet at /service-contact. 8.2 Accessories and spare partsDescription Article no. testo 174H mini data logger, 2-channel, incl. wall bracket, battery (2 x CR 2032 lithium)and calibration protocol0572 6560 testo 174T mini data logger, 1-channel, incl.wall bracket, battery (2 x CR 2032 lithium)and calibration protocol0572 1560 USB interface for programming and readingout testo 174T and testo 174H loggers 0572 0500testo ComSoft CD (if free download requiring registration from website is not desired)0572 0580 Battery, 3 V button cell (CR 2032 lithium),please order 2 batteries per logger 0515 0028ISO calibration certificate humidity, calibration points 11.3 % RH; 50.0 % RH; 75.3 % RH at+25 °C/+77 °F; per channel/instrument0520 0176 ISO calibration certificate temperature,calibration points -18 °C; 0 °C; +60 °C; perchannel/instrument 0520 0151testo AGPostfach 1140, 79849 LenzkirchTesto-Straße 1, 79853 LenzkirchTelefon: (07653) 681-0Fax: (07653) 681-100E-Mail:*************Internet: 0970 1743 de en fr es it pt 01。
阿玛达数冲自动松夹钳代码
阿玛达数冲自动松夹钳代码一、引言阿玛达数冲自动松夹钳是一种用于数控冲床机械手的自动夹钳装置。
它具有高效、精确和稳定的特点，能够实现对工件的自动夹取、松开和定位。
本文将对阿玛达数冲自动松夹钳的代码进行详细讨论，并介绍其在生产制造中的应用。
二、数控冲床机械手的自动夹钳功能数控冲床机械手是一种利用计算机和控制系统来控制机械臂进行自动化操作的设备。
自动夹钳功能作为其重要的一部分，充分发挥了机械手的优势，提高了生产效率和质量。
阿玛达数冲自动松夹钳代码实现了以下主要功能：2.1 自动夹取工件通过编写特定的代码，阿玛达数冲自动松夹钳可以实现对工件的自动夹取。
当机械手定位到指定位置后，通过控制夹钳的运动轴，将夹持装置移动到工件附近，并夹取住工件。
夹取完成后，机械手即可将工件移动到其他指定位置进行下一步操作。
2.2 自动松开工件在完成对工件的加工操作后，阿玛达数冲自动松夹钳可以自动松开工件，以便进行下一步的操作。
通过编写相应的代码，可以控制夹钳松开装置的动作，将夹持工件的力减小或完全松开，使得机械手能够顺利地取下已加工完成的工件。
2.3 自动定位工件阿玛达数冲自动松夹钳代码还可以实现对工件的自动定位。
通过计算工件的尺寸、位置和形状等参数，编写相应的代码，可以使机械手将工件准确地定位到指定的位置。
这样可以确保加工过程中的精度和稳定性，提高工件的质量和生产效率。
三、阿玛达数冲自动松夹钳代码的编写要点编写阿玛达数冲自动松夹钳代码需要考虑以下几个关键要点：3.1 运动轴的控制阿玛达数冲自动松夹钳通过控制运动轴来实现夹取、松开和定位功能。
编写代码时，需要明确每个运动轴的控制方式和运动范围，并将其与工件的位置和姿态相匹配。
对于复杂的工件形状和位置，可能需要结合相关的数学算法和传感器数据进行运动轴的控制。
3.2 传感器数据的处理阿玛达数冲自动松夹钳通常配备有各种传感器，用于感知工件的位置、形状和力量等信息。
编写代码时，需要处理传感器数据，包括采集、滤波、解析和处理等过程。
Sigma 3 in 1 炖水龙头用户指南说明书
2OverviewThank you for purchasing a Sigma 3 in 1 Boiling Water Tap. This provides near boiling 98°C water and standard kitchen hot and cold mixer capabilities from a single tap. This makes it ideal for retro-fit applications as well as new kitchens.The Sigma consists of a tank that is installed under the sink, your chosen Sigma tap (swan or square neck), a scale filter which must be installed and complete installation kit.Each Sigma tap incorporates a safety lock on the boiling side with spring back tap handle to prevent inadvertent dispensing of boiling water.This appliance is intended to be used in household and similar applications such as staff kitchen areas in shops, offices and other working environments.Please fully read these instructions before commencing installation and follow to ensure that installation and operation are simple and safe.1.Important Safety PointsThis appliance can be used by children aged from 12 years and above and persons with reduced physical, sensory or mental capabilities or lack of experience and knowledge if they have been given supervision or instruction concerning use of the appliance in a safe way and understand the hazards involved. Children shall not play with the appliance. Very vulnerable people are not expected to use the appliance safely unless continuous supervision is given.Do not install the appliance if it appears damaged in any way.The appliance is intended for open outlet use only, only connect to the supplied tap as per these instructions.Only connect the appliance to a single-phase supply as specified on the rating plate.Due to the vented nature of this appliance, the tap may drip occasionally during theheating cycle.3This appliance dispenses boiling water.The appliance must be placed on a flat surface where it will not be knocked over causing damage to the water hoses or electrical connections.The appliance must not be used in an area subject to flammable vapours such as paint, solvent or petrol.This appliance is not suitable for outdoor use or in damp conditions.Do not use in ambient temperatures exceeding 35°C.Do not modify or misuse the appliance in any way or serious injury could occur.Do not install the appliance in environments likely to be exposed to freezing conditions.The Sigma must be installed and maintained by a competent person in accordance with current electrical and plumbing regulations.2.Box ContentsElectric instant boiling water tank and kettle lead with 13 amp UK plugSigma tap with silicone tube fittedTap assembly, parts A- E - as shown in diagram 1T aste , odour and scale filterHot flexi hose (red braiding)Cold flexi hose (blue braiding)Flexi hose labelled 'tap to filter'Flexi hose labelled 'filter water to boiler 'Fixing n utB arb ed o utlet c onnectorRed washer43.InstallationImportant InformationThe appliance is intended for open outlet use only, only connect to the supplied tap as per these instructions .Ensure the tap is installed with the mixer tap handle on the right, when stood facing the tap .The installation environment should be adequately ventilated. When installing theappliance, provide approximately 10 - 15cm of air space on the sides of the boiling water tank for air circulation and approximately 2.5cm from the back wall .The tank must be sited vertically and level on a flat surface .As t he appliance is not under pressure there is a slight delay in water flow after the boiling water tap handle has been turned on. This is normal.The supplied scale filter must be fitted and changed at least every 6 months, otherwise the lifespan of the product could be seriously reduced and the warranty will be void .A pressure reducing valve is required if the mains pressure is above 0.5 MPa (5 bar ). Step 1 - Prepare and Site the Tap•Choose a suitable location to mount the tap, this may be an existing hole in your sink or worktop .•If a new hole is required c heck that there will be enough room for the mixer lever to operate and that the reach of the spout will be appropriate for your sink before drilling the tap hole in the sink or worktop. Required hole diameter 32 mm - fits in a standard sink.•See diagram 1 for parts A - E•Take part A. Feed the tap connector ends of the hot (red) and cold (blue) hoses and the hose labelled 'tap to filter ' upwards through part A.•Feed the silicone tube attached to the tap downwards through part A. (Do not screw part A into the tap base yet).•Connect the tap connector ends of the hot (red) and cold (blue) hoses to inlets inside the taplabelled H and C by turning clockwise until a full stop is reached.•Connect the male thread of the hose labelled ‘tap to filter’ to inlet inside the tap labelledT by turning clockwise until a full stop is reached. (T his is the inlet to the right of the T label. The inlet on the left is not required).•T urn threaded stem of part A clockwise into the base of the tap.•Feed all hoses through part B and then locate part B into the corresponding ridge at the base of the main tap.•Feed hoses through the hole in sink/ worktop.•Working on the underside of the sink/worktop, feed all hoses through part C (rubber washer) & D (steel washer) and then locate parts C & D over part A.•Remove screws from part E, place to one side. Place part E around hoses under the worktop. Thread this clockwise onto part A to secure all other parts and tap in place.•Replace screws into part E and tighten with a screwdriver. This will secure the tap in desired position.d iagram 156•Remove protective cover from the filter cartridge. Unscrew the filter housing from the filter nut. Insert filter cartridge into the filter hous ing (see diagram 2) so the cartridge inlet slides into the port of the filter nut . Ensure the black o-ring is inside the filter nut. Screw the filter housing back on to the filter nut.(To replace the filter cartridge after installation ensure instructions in the 'replace filter cartridge' section are followed ).•Consider the length of hose when selecting filter location (diagram 3 shows the installation layout).•Position the filter to ensure hose s will reach from the tap and to the tank. Fix mounting bracket to the wall. Ensure easy access for future maintenance. Ensure that the hoses are not stretched, kinked or twisted.•Detach the blue collars from the inlet and outlet of the filter.•Connect hose labelled ‘tap to filter’ to filter inlet labelled ‘in’, this is a push fit connector.Replace blue collar to the filter inlet to prevent accidental disconnection.•Connect hose labelled ‘filter water to boiler ’ to filter outlet labelled ‘out’, this is a push fit connector. Replace blue collar to the filter outlet to prevent accidental disconnection .Step 2 - Connect the FilterDo not locate the filter above an outlet or other electrical device.d iagram2•A pressure reducing valve is required if the mains pressure is above 0.5 MPa (5 bar).•Unscrew blue cap and remove clear washer from inlet to boiling tank. Connect remaining end of hose labelled ‘filter water to boiler ’ to the blue inlet of the boiling water tank . (Unscrew the yellow cap first and ensure the gauze beneath is used on the inlet connection to prevent debris entering the unit ).•Unscrew red cap and remove clear washer from outlet to boiling tank. Take the red washer, barbed outlet connector , fixing nut and connect to the red outlet of the boiling water tank as per diagram 3.•Push the clear silicone tube, which is already attached to the tap, fully onto the barbed connector. (P lace the tube in a cup of hot water to soften to make connection easier if needed). Check this is secure.•Ensure the silicone tube is not kinked or bent in any way as it will pressurise the tank .•Connect the hot (red) and cold (blue) hoses which are already attached to the tap to the hot and cold mains supply of the buildin g (connection required 1/2" male BSP, not supplied). A dedicated control valve should be installed on the hot and cold mains s upply in a convenient position to facilitate future maintenance.7Step 3 - Prepare Water ConnectionsOnly connect the appliance to supply pressures between 0.15 MPa – 0.5MPa (1.5 -5bar).d iagram38Step 4 - Commission and Check Water Connections4.Operati ng the TapIf the appliance splutters and steams excessively from the tap, the temperature setting should be reduced.•Before plugging the tank into the power socket, the system must be full of water and tested for leaks . Open all water valves to allow water to flow to the instant boiling water tap .•Depress the small button and t urn the spring back instant boiling water handle and hold to fill the tank, this will take approx 1 - 2 minutes . When the tank is full, water will flow from the instant boiling water tap spout . Check water flows smoothly from the tap to ensure all air has been purged from the system.•Turn the instant boiling water handle off and check all connections for any sign of leaks .•To test mixer side of tap, pull large lever on the right side away from the body of the tap.Rotate both ways to dispense hot and cold mains water. Check all connections for any sign of leaks.•Check that all hoses and silicone tubing are not twisted or kinked as this will pressurise the tank . Any leaking joint must be tightened to rectify, and the above procedure undertaken again before connecting to the power socket .Step 5 - Electrical ConnectionEnsure tank is full of water and flushed through before plugging the appliance into the power outlet.•Ensure that the installation area is dry . Connect the kettle style lead to the tank then plug into the power outlet and switch on . Do not use an extension cable .•Press and hold the ON/ OFF icon to switch the tank on, the display panel should light up. The display panel will now show the current tank temperature. Proceed to 'operating the tank' section for further instructions.•For instant boiling water depress the small button on left hand side of the tap. Keep the button pressed down and rotate the handle towards yourself .•As the appliance is not under pressure there is a slight delay in water flow after the boiling water t ap handle has been turned on. This is normal .•For standard hot and cold mixed water, pull the large lever on the right side away from the body of the tap. Rotate for wards to dispense mains hot water and back wards for mainscold water.95.Operating the Boiling TankON/ OFF power controlReduce temp, choose from a number of settings up to 98°CIncrease temp, choose from s ettingsas above•Press and hold the ON/ OFF icon to switch the tank on, the display panel will light up .•Press the increase/ decrease temperature button to select required temperature setting. On first use the temperature will default to 90°C. The maximum temperature setting is 98°C.•When choosing the required temperature using the increase/ decrease button the desired temperature will show on the display and remain there for approx. 8 seconds .•Following this 8 second period the temperature which is displayed will revert to the current tank temperature .•The tank will display the icon 'heating ' when the element is on . Once the desired temperature is reached the icon 'ready' will appear. The tank will take approximately 10 minutes to heat up to 98°C from cold.•Please note that the tank will make a sound like a kettle boiling water whilst heating.This is normal.7.Replace Filter CartridgeTo replace the filter cartridge it is important that you follow the steps below in the correct order (see diagram 2). Before commencing place bowl under filter to catch any small escape of water.Replace the filter cartridge at least every 6 months. This is imperative to protect against scale build up. Failure to do so will void the warranty.10•Replace the filter cartridge at least every 6 months. This is imperative to protect against scale build up. Failure to do so will void the warranty.•Regularly inspect the appliance for any signs of water leaks or damage. If a leak or damage is discovered, discontinue use , isolate the water supply and drain down immediately. C ontact the manufacturer.•T he tap and water tank can be cleaned with a lint free damp cloth. Do not use any abrasive or caustic cleaning products as this will damage the surface of the product .6.MaintenanceBefore conducting any user maintenance, the appliance should first be isolated from the electrical supply by removing the appliance plug from the electrical outlet.Before conducting any maintenance run the boiling tap until it is dispensing cold water. Allow time for tank components to cool.Cleaning and user maintenance shall not be made by children without supervision.If the unit is unused for extended periods of time, it should be unplugged and drained.If there is any risk of the installation environment drop ping below freezing the appliance should be switched of f and drained.1.Grip t-piece bracket with one hand and ridged filter nut in other. ¼ turn clockwise to release filter nut from bracket. This is the auto shut off head which isolates filter nut and housing from bracket. Check that no water is escaping from the t-piece bracket .2.Over a sink bowl grip ridged filter nut in one hand and filter housing in other. Unscrew filter housing and tip to remove old cartridge. Unwrap new cartridge and place in filter housing so cartridge inlet slides into port of filter nut.3.Ensure the black washer is inside the filter nut. S crew the filter housing back into the filter nut. Finally reverse step 1 and push head of filter nut into t-piece bracket and ¼turn anticlockwise to re-connect to water supply.11•U nplug the unit from the electricity mains .•Run the boiling tap until it is dispensing cold water. Allow time for tank components to cool .•Turn off water supply . Disconnect the inlet supply to the tank and remove the silicone hose outlet pipe . Lift the tank to the sink, turn upside down and drain all the water from the tank .•When re-installing follow the original installation instructions .8.DrainingDo not connect to electricity supply without re-filling the tank , checking for leaks and purging any air out of the system . T 01924 225 200F 01924 225 210**************.uk10.Specifications* If pressure is above 0.5 MPa (5 bar ) then a pressure reducing valve is required11.GuaranteeThis product is covered by a standard parts or replacement warranty for a period of 12 months from the date of purchase. If there is a manufacturing defect within the warranty period we will send spare parts, repair and return the unit or, at our discretion, supply a replacement product. Incorrect installation, frost damage , the consequences oflimescale deposits or failure to follow correct operating and maintenance instructions are excluded . Consequential costs such as labour charges or damage to fittings and surroundings are expressly excluded.12.Contact UsIf you experience a problem with this product you should first contact our service department on 01924 225 200 before taking any further action. Experience has shown that issues can often be resolved without the need to return or uninstall the product.Hyco Manufacturing Ltd Normandy Court Express Way Castleford, WF10 5NR。
ISOSLIDE Iron Control Slides 产品说明书
Intended purposeThese “ISOSLIDE ® Iron Control Slides - with reference tissue for the detection of free iron in histological tissue” are used for human-medical cell diagnosis and serve the purpose of the histological investigation and quality control of sample material of human origin. They are ready-to-use glass slides with paraffin sections that contain the typical structures of the corresponding test method. They, when used together with other in vitro diagnostic products from our portfolio, make target structures evaluable for diagnostic purposes (by fixing, embedding, staining, counterstaining, mounting) in human-histological specimen material, for example histologi -cal sections of e. g . the liver .The ISOSLIDE ® Iron Control Slides are prepared using sections of suitable animal material, that clearly visualize ionic iron (Fe 3+) not bound to the heme structure.Each package of ISOSLIDE ® control slides contains 25 slides. The tissue is applied onto the slide next to the white printed labelling field. One slide is already stained with the reference method and is used for comparison. The 24 unstained slides are stained according to the corresponding protocol or with the laboratory’s own protocol.After the staining of the control slides, the staining result is to be compared with the laboratory material and the pre-stained control slide.Due to variable procedures in sampling, fixation, histoprocessing, paraf -finization and equipment, no statements about these processing steps can be made from the ISOSLIDE ® control slides. The ISOSLIDE ® control slides are not quality-controlled for their application with molecular pathology methods and IHC.Unstained structures are relatively low in contrast and are extremely diffi -cult to distinguish under the light microscope. The images created using the staining solutions help the authorized and qualified investigator to better define the form and structure in such cases. Further examinations may be necessary to reach a definitive diagnosis.PrincipleIn the Prussian blue reaction, ionic iron (Fe 3+) not bound to the heme struc-ture reacts with potassium hexacyanoferrate(II) in hydrochloric solution. It precipitates as an insoluble complex salt in the blood, bone-marrow, or tissue cells and thus localizes free cellular iron.4 Fe 3+ + 3 K 4Fe(CN)6 = Fe 4[Fe(CN)6]3 + 12 K +Sample materialParaffin sections with approx. 5 - 6 µm, from tissue samples of animal origin. The tissue was fixed with neutral buffered formalin.ReagentsCat. No. 1.00380.0001 ISOSLIDE ® Iron Control Slideswith reference tissue for the detection of free iron in histological tissue Package components:24 unstained control slides with iron deposits1 stained control slide - stained with the Prussian blue reactionAlso required:Cat. No. 112084 HEMATOGNOST Fe ® 4x 250 ml staining kit for the detection of free ionic iron (Fe 3+) in cellsSample preparationThe sampling must be performed by qualified personnel.All samples must be treated using state-of-the-art technology. All samples must be clearly labeled.Suitable instruments must be used for taking samples and their prepara-tion. Follow the manufacturer’s instructions for application / use.In Vitro Diagnostic Medical DeviceWhen using the corresponding auxiliary reagents, the corresponding in -structions for use must be observed.Deparaffinize sections in the conventional manner and rehydrate in a de -scending alcohol series.ProcedureAnalogous to Cat. No. 112084 - HEMATOGNOST Fe ® staining kit:The staining kit includes 3 reagents:Reagent 1: HEMATOGNOST Fe ® Potassium hexacyanoferrate(II) solution 250 ml Reagent 2: HEMATOGNOST Fe ® Hydrochloric acid 250 ml Reagent 3: HEMATOGNOST Fe ® Nuclear fast red solution 2x 250 mlPrepare the reagents according to the protocol. Stain the slides in a staining cell.Carrying out the Prussian blue reaction at 37 °C yields a sharper definition of the stain precipitates.After dehydration (ascending alcohol series) and clarification with xylene or Neo-Clear ®, histological slides can be covered with non-aqueous mounting agents (e. g . Entellan ® new, Neo-Mount ®) and a cover glass and can then be stored.The staining will remain stable for several month.The use of immersion oil is recommended for the analysis of stained slides with a microscopic magnification >40x.ResultFree iron (Fe 3+)intensive blue granules Nucleipale redCytoplasmtender pinkTechnical notesThe microscope used should meet the requirements of a medical diagnostic laboratory.When using histoprocessor systems or automatic staining systems, please follow the instructions for use supplied by the supplier of the system and software.Remove surplus immersion oil before filing.DiagnosticsDiagnoses are to be made only by authorized and qualified personnel. Valid nomenclatures must be used.This method can be supplementarily used in human diagnostics.Further tests must be selected and implemented according to recognized methods.Suitable controls should be conducted with each application in order to avoid an incorrect result.StorageStore the ISOSLIDE ® Iron Control Slides - with reference tissue for the detection of free iron in histological tissue at +15 °C to +25 °C.Shelf-lifeThe ISOSLIDE ® Iron Control Slides - with reference tissue for the detection of free iron in histological tissue can be used until the stated expiry date. The package can be used up to the stated expiry date when stored at +15 °C to +25 °C.CapacityThe package is sufficient for 24 applications.Additional instructionsFor professional use only.In order to avoid errors, the application must be carried out by qualified personnel only.National guidelines for work safety and quality assurance must be followed. Microscopes equipped according to the standard must be used.Protection against infectionEffective measures must be taken to protect against infection in line with laboratory guidelines.Instructions for disposalThe kit and any unused control slides must be disposed of in accordance with the current disposal guidelines.Used solutions and solutions that are past their shelf-life must be disposed of as special waste in accordance with local guidelines. Information on dis- posal can be obtained under the Quick Link “Hints for Disposal of Microsco-py Products” at . Within the EU the currently applicable REGULATION (EC) No 1272/2008 on classification, labelling and packaging of substances and mixtures, amending and repealing Directives 67/548/EEC and 1999/45/EC, and amending Regulation (EC) No 1907/2006 applies.Microscopy ISOSLIDE ® Iron Control Slideswith reference tissue for the detection of free iron in histological tissue1.00380.0001For professional use onlyAuxiliary reagentsCat. No. 103699 Immersion oil Type N acc. to ISO 8036 100-ml drop-for microscopy ping bottle Cat. No. 104699 Immersion oil 100-ml drop- for microscopy ping bottle,100 ml, 500 ml Cat. No. 107961 Entellan ® new 100 ml, 500 ml, rapid mounting medium 1 l for microscopyCat. No. 108298 Xylene (isomeric mixture) 4 lfor histology Cat. No. 109016 Neo-Mount ® 100-ml drop- anhydrous mounting medium ping bottle, for microscopy500 ml Cat. No. 109843 Neo-Clear ® (xylene substitute) 5 l for microscopyCat. No. 112084 HEMATOGNOST Fe ® 4x 250 mlstaining kit for the detection of free ionic iron (Fe 3+) in cellsHazard classificationCat. No. 1.00380.0001Please observe the hazard classification printed on the label and the infor -mation given in the safety data sheet.The safety data sheet is available on the website and on request.Main product componentsCat. No. 1.00380.0001Paraffin sections with animal tissue: 25 slidesOther IVD productsCat. No. 100361 ISOSLIDE ® Reticulin Control Slides 25 tests with reference tissue for the detection of reticular fibres in histology Cat. No. 100408 ISOSLIDE ® PAS Control Slides 25 tests with reference tissue for the detection of polysaccharides in histological tissue Cat. No. 100425 ISOSLIDE ® Alcian blue, pH 2.5 25 tests Control Slides with reference tissue for the detection of acid mucosubstances in histological tissueCat. No. 102472 ISOSLIDE ® Warthin-Starry Control Slides 25 tests with reference tissue for the detection of Helicobacter pylori and Spirochetes in histological tissueCat. No. 102473 ISOSLIDE ® Methenamine Control Slides 25 tests with reference tissue for the detection of argent-affine structures in histological tissueCat. No. 102560 ISOSLIDE ® AFB Control Slides 25 tests with reference tissue for the detection of acid-fast bacteria in histological tissueGeneral remarkIf during the use of this device or as a result of its use, a serious incident has occurred, please report it to the manufacturer and/or its authorised representative and to your national authority.Literature1. R omeis - Mikroskopische Technik, Editors: Maria Mulisch, Ulrich Welsch, 2015, Springer Spektrum, 19. Auflage2. W elsch Sobotta - Lehrbuch Histologie, Editor: Ulrich Welsch, 2006, ELSEVIER Urban&Fischer , 2. Auflage3. Histotechnik, Gudrun Lang, 2013 Springer Verlag, 2. Auflage4. T heory and Practice of Histological Techniques, John D Bancroft, Marilyn Gamble, 2008, Churchill Livingstone ELSEVIER, 6th Edition5. Laboratory Manual of Histochemistry, Linda L. Vacca, 1985, Raven Press6. Staining Procedures, George Clark, 1981, Williams&Wilkins, 4th Edition7. B asiswissen Histologie und Zytologie, Karl Heinz Stein, Hellmut Flenker , 1998, uZv, 2. Auflage8. H istological & Histochemical Methods: Theory & Practice, J. A. Kiernan, 1990, Pergamon Press, 2nd Edition9. H istological and Histochemical Methods, Theory and practice, J. A. Kiernan, 2015, Scion Publishing Ltd, 5th EditionConsult instructions for useTemperature limitationUse byYYYY-MM-DD Caution, consultaccompanying documents Manufacturer Catalog number Batch codeStatus: 2021-Jan-06Merck KGaA, 64271 Darmstadt, Germany, Tel. +49(0)6151 72-2440EMD Millipore Corporation, 400 Summit DriveBurlington MA 01803, USA, Tel. +1-978-715-4321Sigma-Aldrich Canada Co. or Millipore (Canada) Ltd. 2149 Winston Park, Dr . Oakville, Ontario, L6H 6J8 Phone: +1 800-565-1400。
ascii码表
86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124
56 57 58 59 5A 5B 5C 5D 5E 5F 60 61 62 63 64 65 66 67 68 69 6A 6B 6C 6D 6E 6F 70 71 72 73 74 75 76 77 78 79 7A 7B 7C
2F 30 31 32 33 34 35 36 37 38 39 3A 3B 3C 3D 3E 3F 40 41 42 43 44 45 46 47 48 49 4A 4B 4C 4D 4E 4F 50 51 52 53 54 55
/ 0 1 2 3 4 5 6 7 8 9 : ; < = > ? @ A B C D E F G H I J K L M N O P Q R S T U
01111101 01111110 01111111
175 176 177
125 126 127
7D 7E 7F
} ~ DEL (delete)
闭花括号 波浪号 删除
Bin(二进 制) 0000 0000 0000 0001 0000 0010 0000 0011 0000 0100 0000 0101 0000 0110 0000 0111 0000 1000 0000 1001 0000 1010 0000 1011 0000 1100 0000 1101 0000 1110 0000 1111 0001 0000 0001 0001 0001 0010 0001 0011 0001 0100 0001 0101 0001
- 1、下载文档前请自行甄别文档内容的完整性，平台不提供额外的编辑、内容补充、找答案等附加服务。
- 2、"仅部分预览"的文档,不可在线预览部分如存在完整性等问题,可反馈申请退款(可完整预览的文档不适用该条件!)。
- 3、如文档侵犯您的权益，请联系客服反馈,我们会尽快为您处理(人工客服工作时间：9:00-18:30)。
History of PIV Developmentin JAPANKoji OKAMOTOThe University of Tokyookamoto@k.u-tokyo.ac.jpokamoto@Flow Visualization Society of Japan•Founded in 1981 (National Workshop from 1973)•1st Int. Symp. Flow Visualization, 1977 Tokyo•1st president: Prof. T. Asanuma•Digital image analysis on Visualized images –1981 K.Ohmi: Particle Streak Image–1982 T. Kobayashi: Particle Streak Image–1982 M. Yano: Particle Tracking Velocimetry–1983 M. Yano: Cross-correlation PIVhttp://www.journalarchive.jst.go.jp/english/jnltop_en.php?cdjournal=jvs1981M. Yano (1983) “Velocity Measurement using Correlation concerning with Digital Tracer Image,”J. Flow Vis. Soc. Jpn, 3, pp.189-193 Tracer image before unit time Tracer image after unit timeCross correlation of thetracer imagesVelocity distributionDevelopment of PIV in 1980s •2D PIV development–1986 I. Kimura: Direct Cross-correlation (window shift)–1986 T.Uemura: nearest pair PTV–1987 M.Kawahashi: Digital Laser Speckle Technique–1987 A. Kaga: Intensity Substitution–1988 T. Uemura, F.Yamamoto: Binary Cross-correlation •3D3C measurement–1986 J.Doi: Stereo Analysis of Hydrogen bubble–1987 K. Nishino, N. Kasagi: 3D PTV–1987 T. Kobayashi: 3D PTVNishino, K.,Kasagi, N. and Hirata M., (1989), “Three-dimensional Particle Tracking Velocimetry Based on Automated Digital Image Processing,”Trans. ASME, J. Fluid Engineering,111, pp.384-390.Kasagi N. and Nishino K. 1990, “Probing Turbulence with three-Dimensional Particle Tracking Velocimetry,”Experimental Thermal Fluid Science, 4,pp.601-612Visualization Society of Japan •Founded in 1990 (FVS VSJ)•International Workshop on PIV–1995 Fukui Profs. Kobayashi and Yamamoto–1997 Fukui Profs. Kobayashi and Yamamoto–Santa Barbara, Göttingen, Busan, Pasadena,Rome, Melbourne, Tsukuba,…..•Research Committee on PIV(1996-2000)–Standard Experiment Project–Standard Image Project (www.piv.jp)–Handbook of PIV (2002)–2000s: Dynamic PIV / Micro Visualization / ….PIV Standard Images (1996)#01 –1#04 –1www.piv.jpFor High-speed 2D PIVFor 3D PTVFor High-speedStereo PIV 3D-STD-PIV(1999)For High-speed 2D PIV (#301)www.piv.jp 3D-STD-PIV(1999)For High-speed 3D-PTV or Stereo PIV (#351)Three cameras with different view angleLaser light sheet illuminationImage distortion by test section surfacewww.piv.jp 3D-STD-PIV(1999)For 3D PTV (#337)www.piv.jp 3D-STD-PIV(1999)PIV Developments in 2000s •PIV Hardware–Dynamic PIV (high-speed PIV)–Confocal scanner micro-PIV•PIV Software–Multi-image PIV (3-10 images / temporal sampling)–High-order PIV (Acceleration estimation)•Application Extensions–Micro-PIV, Nano-PIV–Sound Visualization–Bioengineering–and so on…..Photron FastCAM APX SA5 1024 x 1024 7,000fps(150µs)1024 x 74410,000fps(100µs)832 x 44820,000fps( 50µs)512 x 32042,000fps( 24µs)320 x 26475,000fps( 13µs)CMOS, 12bit gray scaleExternal synchronize (0-1,300,000Hz)Dead time (~1µs)http://www.photron.co.jpCASIO EXILIM EX-F1 1280x 720 30fps(33ms)512 x 384300fps(3.3ms)432 x 192600fps(1.7ms)336 x 961,200fps(0.8ms)CMOS, 6Mpixel78,000JPY (~$800)http://dc.casio.jp/product/exilim/ex_f1/10,000Hz (∆t=100µs)Evolution-30 10kHz(single mode)FastCAM MAX 512x256 / 10,000fpsJan. 2003Velocity distribution (10,000Hz)Fine structure / Motion of vortexApplications for Dynamic PIV •Micro-PIV (Dynamic Structure in micro-fluidics)•Scanning micro-PIV (3D micro-system)•Holographic PIV (3D structure)•Biological PIV (Transient Blood flow)•Multi-point correlation(10,000 LDV with 10kHz)•Spatial dynamic range (double camera /1:600)•Tomographic Reconstruction (multi-camera)•Visualization of Sound(High frequency vibration 100~1000Hz)50100150Shinohara et al. 2007Butyl AcetateWater t = 90.0 mst = 90.5 mst = 91.0 ms400400 µm/s400 µm/s Shinohara et al. 2007Out-of-plane velocityDual-plane PIV system for macro-scaleRaffel et al(1995) Exp.Fluids1969-77Shinohara et al. 20073D3C (x,y,z,u,v,w)Shinohara et al. 2007PIV Developments in 2010s •PIV Hardware–Dynamic PIV (high-speed PIV)–Confocal scanner micro-PIV •PIV Software–Multi-image PIV–High-order PIV •Application Extensions–Micro-PIV, Nano-PIV–Sound Visualization–Bioengineering–and so on…..1k x 1k @ 10kHz camera 10mJ/pulse @ 10kHz laserHigh temporal/spatial resolution analysis technique Advanced Technologyto protect Global Warming….。