学术会议海报模板

Results
Table 1 Analysis of components of CSS Time/min 4.43 5.06 5.41 5.79 6.22 6.65-7.60 7.90 8.49-9.84 M 1000 1146 1188 1002 1148 1190 1044 1086 1232 Formula C45H76O24 C51H86O28 Unknown C45H78O24 C51H88O28 C55H98O27 C33H58O15 Unknown C55H92O30 [M-H]999.51 1145.58 1187.41 1147.60 1189.77 1043.54 1085.70 1231.62 [M+Cl]- [M+Na]+ 1035.49 1023.55 1181.57 1169.63 1223.46 1211.50 1025.56 1171.63 1213.71 1067.59 1109.64 1255.68 1183.56 1225.73 1079.55 1121.68 1267.61 Type MukuroziosideⅠb MukuroziosideⅡb Impurity MukuroziosideⅠa MukuroziosideⅡa Unknown Unknown Impurity Mukurozioside A Abs 1.0 0.5
Introduction
Sapindus saponin has wonderful detergency which is natural, environment-frendly and nontoxic. It can be developed into food industry cleaning agent. However, there is no pure sapindus saponin commercially available. We produced Total sapindus-saponins, the purity were calculated to more than 95.2%. TSS were mainly oleanolic acid saponins and can be used for quality control as standard. No. 1 2 3 4 5 6 7 8
1206 1074 1128 1400 882 750 924 924 966 966
C58H94O26 C53H86O22 C56H88O33 Unknown C46H74O16 C41H66O12 C48H76O17 C48H76O17 C50H78O18 C50H78O18
1205.68 1073.61 1127.71 1399.72 881.51 749.47 923.53 923.65 965.56 965.54
Preperation of total sapindus-saponins for quality control
H. Wu, C. Zhang, D. P. Xu, H. Y. Wang, W. R. Yao
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
1241.67 1109.59 1163.70 1436.70 918.51 785.45 959.52 959.63 1001.54 1001.52
1129.70 1097.63 1151.69 1423.74 905.53 773.49 947.55 947.65 989.53 989.54
Mukurozi-saponinY Mukurozi-saponinX Unknown Impurity Sapindoside B Sapindoside A Mukurozi-saponin E1 Sapindoside L Mukurozi-saponin G Sapindoside M
Conclusion
It can be further concluded that TSS were mainly oleanolic acid saponins and can be used for quality control as standard. Author: Heng Wu, Master degree Email: wuheng369414949@
15000
10000
11
0 4 8 12 16 Time/min
0
4Байду номын сангаас
8
Fig 1 ESI- ion chromatograms of CSS
12 16 Time/min
Fig 2 The result of separation of MCI GEL by TLC detection, the hemolytic test and ESI- ion chromatograms (Left Right)
0.0 400.0
9
10 11 12 13 14 15
10.30
11.20 13.76 14.72 15.00 15.14 15.52 15.73 16.06
Methods
Sapindus Solvant TSS Qualitative test: pericarp extract Hemolysis test. AB-8 macroporous resin Crude sapindus- MCI Total sapindussaponins (CSS) Gel saponins (TSS) TSS Quantitive test: High Performance Liquid Chromatography/Mass spectrum,vanillin–sulfuric acid.
Results
Ion current 30000 25000 5 20000 4 23 1 6 7 8 9 10 11 14 13 15 12
Hemolysis test of “c” proved the most hemolytic. The components 11~16 were analysed by HPLC/MS, identified as Oleanolic acid saponins. (Table 1, Fig 2) Ion current 45 25000 a a 6 2 3 1 7 20000 15000 b 10000 c 5000 c 0 b 8
c
396
952
95.2
910 14 15 13 12
The purity of TSS (Total Sapindus Saponin) were calculated to more than 95.2% by acid hydrolysis under the condition of high temperature and pressure. (Table 2)
Results
1.5 sapindus-sapogenin 548.0,1.0
549.0,0.9 Olenolic acid
450.0 500.0
1001.52 1037.50
550.0 600.0 Wave(nm) Fig 3 The UV graph of Olenolic acid and sapindus-sapogenin in vanilin-acetic acid-HClO4 system Oleanolic acid was chosen as standard. Sapogenin of TSS after hydrolysis had a maximum absorption at 550 nm with the method of vanillin– sulfuric acid, which was the same as Oleanolic acid. (Fig 3) Table 2 Comparation of hydrolysis conditions of the total sapindussaponins (a-Heatunderreflux,b-100℃ water bath,c-High temperature and pressure) Hydrolysis Triterpinoid TSS TSS conditions sapogenin(mg) (mg) purity(%) a b 370 336 888 806 88.8 80.6