The nuclear acid amplification modulo which design

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专利名称:The nuclear acid amplification modulo which designates the not yet refinement blood as
the sample
发明人:中山 尚母,中山 雅人
申请号:JP2007124454
申请日:20070509
公开号:JP5292722B2
公开日:
20130918
专利内容由知识产权出版社提供
摘要:
To provide a method for amplifying nucleic acid from a whole blood sample (frozen sample or non-frozen FRESH sample) without purifying the nucleic acid and by which impurities inhibiting the nucleic acid amplifying reaction can simply be neutralized by a chemical treatment to facilitate the nucleic acid amplifying reaction from the blood.
SOLUTION: This method for amplifying the nucleic acid comprises a pretreatment process for mixing the whole blood sample with a pretreatment reagent prepared in prescribed concentration, a dilution mixing process for dilution-mixing the mixture solution obtained from the pretreatment process with a nucleic acid amplifying reaction solution and a nucleic acid amplifying process for performing the nucleic acid amplifying reaction in the dilution mixture solution obtained in the dilution mixing process.
COPYRIGHT: (C)2009 and JPO& INPIT
申请人:凸版印刷株式会社
地址:東京都台東区台東1丁目5番1号
国籍:JP
代理人:特許業務法人高橋・林アンドパートナーズ更多信息请下载全文后查看。

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