淫羊藿苷对人骨关节炎软骨细胞增殖凋亡的影响

淫羊藿苷对人骨关节炎软骨细胞增殖凋亡的影响
赵晓燕
【期刊名称】《海南医学》
【年(卷),期】2018(029)001
【摘要】目的研究淫羊藿苷对人骨关节炎软骨细胞增殖凋亡的影响.方法体外分
离培养人骨关节炎软骨细胞,用不同浓度的淫羊藿苷作用于人骨关节炎软骨细胞,噻
唑蓝(MTT)法检测细胞增殖,流式细胞术检测细胞凋亡,酶联免疫吸附测定法(ELISA)检测细胞培养液上清中白细胞介素-1β(IL-1β)水平,Western blot检测细胞中基质
金属蛋白酶-1(MMP-1)、基质金属蛋白酶-3(MMP-3)、基质金属蛋白酶-
13(MMP-13)的表达.结果 0、50μg/mL、100μg/mL、150μg/mL的淫羊藿苷作
用后的骨关节炎软骨细胞OD值依次为(0.58±0.04)、(0.76±0.06)、(0.92±0.07)、(1.26±0.09),凋亡率依次为(9.35±0.27)％、(8.69±0.11)％、(7.41±0.12)％、
(6.87±0.09)％,细胞分泌的IL-1β依次为(10.53±0.97)pg/mL、
(7.94±0.84)pg/mL、(6.05±0.71)pg/mL、(5.02±0.49)pg/mL,MMP-1水平依次为(0.25±0.02)、(0.20±0.01)、(0.16±0.01)、(0.11±0.02),MMP-3水平依次为(0.11±0.01)、(0.08±0.01)、(0.05±0.01)、(0.02±0.01),MMP-13水平依次为(0.39±0.03)、(0.30±0.02)、(0.21±0.01)、(0.19±0.01).以上各项指标比较,差异
均有统计学意义(P<0.05).结论淫羊藿苷能够促进人骨关节炎软骨细胞增殖,抑制人骨关节炎软骨细胞凋亡,作用机制可能与细胞分泌的IL-1β和细胞中MMP-1、MMP-3、MMP-13的表达水平有关.%Objective To study the effects of icariin on the proliferation and apoptosis of human osteoarthritis chondrocytes. Methods Human osteoarthritis chondrocytes were isolated and cultured in
vitro, and then treated with different concentrations of icariin. Cell proliferation was detected by MTT assay, and cell apoptosis was detected by flow cytometry. Interleukin-1beta (IL-1β) in supernatant of cell culture was detected by ELISA, and western blot was used to detect the expression of matrix metalloproteinase-1 (MMP-1), MMP-3 and MMP-13 in the cells. Results After treat-ment with 0 μg/mL, 50 μg/mL, 100 μg/mL and 150
μg/mL icariin, the OD values were (0.58±0.04), (0.76±0.06), (0.92± 0.07), (1.26±0.09); the rate of apoptosis were (9.35±0.27)％, (8.69±0.11)％, (7.41±0.12)％, (6.87±0.09)％; the secretion levels of IL-1β were (10.53±0.97) pg/mL, (7.94±0.84) pg/mL, (6.05±0.71) pg/mL, (5.02±0.49) pg/mL; MMP-1 expres-sion levels were (0.25±0.02), (0.20±0.01), (0.16±0.01), (0.11±0.02); MMP-3 expression levels were (0.11±0.01), (0.08± 0.01), (0.05±0.01), (0.02±0.01); MMP-13 expression levels were (0.39±0.03), (0.30±0.02), (0.21±0.01), (0.19±0.01). There were statistically significant differences in the above indexes (P<0.05). Conclusion Icariin can promote the pro-liferation and inhibit the apoptosis of human osteoarthritis chondrocytes. The mechanism might involve the expression of MMP-1, MMP-3 and MMP-13 and the levels of IL-1β secreted by the cells.
【总页数】4页(P10-13)
【作者】赵晓燕
【作者单位】沈阳药科大学药事管理专业,辽宁沈阳 110015;中国人民解放军总医院门诊药局,北京 100853
【正文语种】中文
【中图分类】R684.3
【相关文献】
1.基于TGF-β1/CTGF信号通路探讨山茱萸总甙对人骨关节炎软骨细胞增殖和凋亡的影响 [J], 王冠贤;李宝林;唐爱民;黄永湘
2.毛蕊异黄酮对人骨关节炎软骨细胞增殖和凋亡的影响 [J], 廖军锋;王虹;吕晓宇
3.miR-375在骨关节炎软骨中的表达及对软骨细胞增殖和凋亡的影响 [J], 刘旭;陈洪涛;那次克道尔吉;何云;曹力
4.环状RNA hsa_circ_0023404对人骨关节炎软骨细胞增殖、凋亡的影响及其机制 [J], 李晓英;吴涛;陈曼丽;张翠召;宫蕊
5.大蒜素对人膝骨关节炎软骨细胞增殖与凋亡的影响及机制研究 [J], 赵林芳;韩爱敏
因版权原因，仅展示原文概要，查看原文内容请购买。