siRNA干扰CXCR4基因对T24细胞侵袭力的影响

siRNA干扰CXCR4基因对T24细胞侵袭力的影响
郑湘予;庞霞;李晟磊;朱志强
【期刊名称】《郑州大学学报（医学版）》
【年(卷),期】2012(047)005
【摘要】目的:研究RNA干扰下调CXCR4基因对膀胱移行细胞癌T24细胞体外侵袭力的影响.方法:根据CXCR4基因特点,设计合成CXCR4特异性小干扰
RNA(siRNA),并转染T24细胞,同时设置空白对照(转染时只加脂质体)和阴性对照(采用无关序列RNA转染)组.通过RT-PCR方法检测CXCR4 mRNA的表达,采用Western blot方法检测CXCR4蛋白的表达,用Boyden小室体外细胞侵袭模型检测细胞体外侵袭能力.结果:各组T24细胞的CXCR4 mRNA、蛋白水平和穿膜细胞数比较,差异有统计学意义(F=140.822、473.079和133.247,P<0.001).siRNA转染组T24细胞的CXCR4 mRNA、蛋白表达下调,体外侵袭力减弱(P<0.05).结论:CXCR4特异性siRNA可降低膀胱移行细胞癌T24细胞体外侵袭力.%Aim: To investigate the effects of CXCR4 gene silencing by small interfering RNA on invasiveness of bladder cancer T24 cells. Methods:Using Lipofectamine?2000,CXCR4 siRNA was transfected into T24 cells in
vitro( siRNA transfect group ). Blank control group and negative control group were established. RT-PCR was used to detect the expression of CXCR4 mRNA in T24 cells. Westerm blot was used to detect the expression of CXCR4 protein. Boyden chamber experiment was used to count the membrane-permeable cells. Results:The levels of CXCR4 mRNA and protein expression, and the number of membrane-permeable cells among
the 3 groups had significant differences( F = 140. 822,473. 079, 133. 247,P <0. 001 ),which were lower in siRNA transfect group than those in the control groups, and the invasive ability of the cells in siRNA transfect group decreased( P > 0. 05 ). Conclusion: Using siRNA to down-regulate CXCR4 gene can reduce the invasive and proliferative power of T24 cell line.
【总页数】3页(P667-669)
【作者】郑湘予;庞霞;李晟磊;朱志强
【作者单位】郑州大学第一附属医院病理科,郑州,450052;郑州大学第一附属医院病理科,郑州,450052;郑州大学第一附属医院病理科,郑州,450052;郑州大学第一附属医院急诊科,郑州,450052
【正文语种】中文
【中图分类】R737.1
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