slat127a
html颜色表及代码
颜色名中文名称Hex RGB 十进制Decimal LightPin k 浅粉红#FFB6C1255,182,193Pink 粉红#FFC0CB 255,192,203Crimson 深红(猩红)#DC143C 220,20,60Lavender Blush淡紫红#FFF0F5255,240,245PaleViol etRed 弱紫罗兰红#DB7093219,112,147HotPink 热情的粉红#FF69B4255,105,180DeepPink 深粉红#FF1493255,20,147MediumVi oletRed 中紫罗兰红#C71585199,21,133Orchid 暗紫色(兰花紫)#DA70D6218,112,214Thistle 蓟色#D8BFD8216,191,216Plum 洋李色(李子紫)#DDA0DD 221,160,221Violet 紫罗兰#EE82EE 238,130,238Magenta 洋红(玫瑰红)#FF00FF 255,0,255Fuchsia 紫红(灯笼海棠)#FF00FF 255,0,255DarkMage nta深洋红#8B008B 139,0,139Purple 紫色#800080128,0,128MediumOr chid中兰花紫#BA55D3186,85,211DarkViol et暗紫罗兰#9400D3148,0,211DarkOrch id暗兰花紫#9932CC 153,50,204Indigo 靛青/紫兰色#4B008275,0,130BlueViol et蓝紫罗兰#8A2BE2138,43,226MediumPu rple中紫色#9370DB 147,112,219MediumSl ateBlue 中暗蓝色(中板岩蓝)#7B68EE 123,104,238SlateBlu e 石蓝色(板岩蓝)#6A5ACD 106,90,205DarkSlat eBlue 暗灰蓝色(暗板岩蓝)#483D8B 72,61,139Lavender 淡紫色(熏衣草#E6E6FA 230,230,250GhostWhi te幽灵白#F8F8FF 248,248,255Blue 纯蓝#0000FF 0,0,255html颜色表及代码颜色名称及色样表(HTML版)MediumBlue中蓝色#0000CD0,0,205 MidnightBlue午夜蓝#19197025,25,112 DarkBlue暗蓝色#00008B0,0,139 Navy海军蓝#0000800,0,128RoyalBlu e 皇家蓝/宝蓝#4169E165,105,225CornflowerBlue矢车菊蓝#6495ED100,149,237 LightSteelBlue亮钢蓝#B0C4DE176,196,222LightSla teGray 亮蓝灰(亮石板#778899119,136,153SlateGra y 灰石色(石板灰)#708090112,128,144DodgerBl ue 闪兰色(道奇蓝)#1E90FF30,144,255AliceBlu e 爱丽丝蓝#F0F8FF240,248,255SteelBlu e 钢蓝/铁青#4682B470,130,180LightSkyBlue亮天蓝色#87CEFA135,206,250 SkyBlue天蓝色#87CEEB135,206,235DeepSkyB lue 深天蓝#00BFFF0,191,255LightBlu e 亮蓝#ADD8E6173,216,230PowderBl ue 粉蓝色(火药青)#B0E0E6176,224,230CadetBlu e 军兰色(军服蓝)#5F9EA095,158,160Azure蔚蓝色#F0FFFF240,255,255LightCya n 淡青色#E0FFFF224,255,255PaleTurquoise弱绿宝石#AFEEEE175,238,238 Cyan青色#00FFFF0,255,255Aqua 浅绿色(水色)#00FFFF0,255,255DarkTurquoise暗绿宝石#00CED10,206,209DarkSlat eGray 暗瓦灰色(暗石板灰)#2F4F4F47,79,79DarkCyan暗青色#008B8B0,139,139 Teal水鸭色#0080800,128,128 MediumTurquoise中绿宝石#48D1CC72,209,204LightSea Green 浅海洋绿#20B2AA32,178,170Turquois e 绿宝石#40E0D064,224,208Aquamarine宝石碧绿#7FFFD4127,255,212MediumAq uamarine 中宝石碧绿#66CDAA102,205,170MediumSp ringGree 中春绿色#00FA9A0,250,154MintCream薄荷奶油#F5FFFA245,255,250SpringGr een春绿色#00FF7F 0,255,127MediumSe aGreen中海洋绿#3CB37160,179,113SeaGreen 海洋绿#2E8B5746,139,87Honeydew 蜜色(蜜瓜色)#F0FFF0240,255,240LightGre en 淡绿色#90EE90144,238,144PaleGree n 弱绿色#98FB98152,251,152DarkSeaG reen 暗海洋绿#8FBC8F 143,188,143LimeGree n 闪光深绿#32CD3250,205,50Lime 闪光绿#00FF000,255,0ForestGr een森林绿#228B2234,139,34Green 纯绿#0080000,128,0DarkGree n 暗绿色#0064000,100,0Chartreu se 黄绿色(查特酒#7FFF00127,255,0LawnGree n 草绿色(草坪绿_#7CFC00124,252,0GreenYel low绿黄色#ADFF2F 173,255,47DarkOliv eGreen暗橄榄绿#556B2F 85,107,47YellowGr een 黄绿色#9ACD32154,205,50OliveDra b 橄榄褐色#6B8E23107,142,35Beige 米色/灰棕色#F5F5DC 245,245,220LightGoldenrodYe llow亮菊黄#FAFAD2250,250,210Ivory 象牙色#FFFFF0255,255,240LightYel low浅黄色#FFFFE0255,255,224Yellow 纯黄#FFFF00255,255,0Olive 橄榄#808000128,128,0DarkKhak i 暗黄褐色(深卡叽布)#BDB76B 189,183,107LemonChi ffon柠檬绸#FFFACD 255,250,205PaleGold enrod 灰菊黄(苍麒麟#EEE8AA 238,232,170Khaki 黄褐色(卡叽布)#F0E68C 240,230,140Gold 金色#FFD700255,215,0Cornsilk 玉米丝色#FFF8DC 255,248,220Goldenro d 金菊黄#DAA520218,165,32DarkGold enrod暗金菊黄#B8860B 184,134,11FloralWh ite 花的白色#FFFAF0255,250,240OldLace 老花色(旧蕾丝)#FDF5E6253,245,230Wheat 浅黄色(小麦色)#F5DEB3245,222,179Moccasin 鹿皮色(鹿皮靴)#FFE4B5255,228,181Orange橙色#FFA500255,165,0PapayaWh ip 番木色(番木瓜)#FFEFD5255,239,213BlanchedAlmond白杏色#FFEBCD255,235,205NavajoWh ite 纳瓦白(土著白)#FFDEAD255,222,173AntiqueWhite古董白#FAEBD7250,235,215 Tan茶色#D2B48C210,180,140BurlyWoo d 硬木色#DEB887222,184,135Bisque陶坯黄#FFE4C4255,228,196 DarkOrange深橙色#FF8C00255,140,0 Linen亚麻布#FAF0E6250,240,230 Peru秘鲁色#CD853F205,133,63PeachPuf f 桃肉色#FFDAB9255,218,185SandyBro wn 沙棕色#F4A460244,164,96Chocolat e 巧克力色#D2691E210,105,30SaddleBr own 重褐色(马鞍棕#8B4513139,69,19Seashell海贝壳#FFF5EE255,245,238 Sienna黄土赭色#A0522D160,82,45LightSal mon 浅鲑鱼肉色#FFA07A255,160,122Coral珊瑚#FF7F50255,127,80OrangeRe d 橙红色#FF4500255,69,0DarkSalm on 深鲜肉/鲑鱼色#E9967A233,150,122Tomato番茄红#FF6347255,99,71MistyRos e 浅玫瑰色(薄雾玫瑰)#FFE4E1255,228,225Salmon 鲜肉/鲑鱼色#FA8072250,128,114Snow雪白色#FFFAFA255,250,250LightCor al 淡珊瑚色#F08080240,128,128RosyBrow n 玫瑰棕色#BC8F8F188,143,143IndianRe d 印度红#CD5C5C205,92,92Red纯红#FF0000255,0,0 Brown棕色#A52A2A165,42,42FireBric k 火砖色(耐火砖)#B22222178,34,34DarkRed深红色#8B0000139,0,0 Maroon栗色#800000128,0,0 White纯白#FFFFFF255,255,255 WhiteSmoke白烟#F5F5F5245,245,245Gainsbor o 淡灰色(庚斯博#DCDCDC220,220,220LightGre y 浅灰色#D3D3D3211,211,211Silver银灰色#C0C0C0192,192,192 DarkGray深灰色#A9A9A9169,169,169 Gray灰色#808080128,128,128DimGray 暗淡的灰色#696969105,105,105Black纯黑#0000000,0,0。
Titan 平台安全系列产品介绍说明书
United Kingdom PartnersWhere To BuyProduct NumbersDetails 1011895Titan roofers kitTitan Roofer kit composed of : * 2-point Titan harness with rear & front anchorage (front webbing loops) * Titan Automatic rope grab with 10manchorage line and 30cm attachment lanyard1011896Titan fall arrest kitKit composed of : * Titan 1-point harness with rear and sternalanchorage. * 2m Titan shock absorbing lanyard with 2 screwgatekarabiners1011897Titan restraint kitThis kit is not suitable for fall arrest! This kit is ideal for flat roofmaintenance,MEWPS... Composition : * Titan 1 point harness with rearanchorage. * Titan F 2 M restraint lanyard with 2 screwgatekarabiners. * Titan 1-point harness, rear anchorage, 2m restraintlanyard with 2 screwgate karabiners1011898Titan construction kitConstruction kit composed of : * Titan 1-point harness, rear anchorage*Titan CMS personal fall arrester with 2.5m webbing lifeline and 2screwgate karabiners.1011899Titan work positioning kitThis Kit is composed of : * Titan 2-point harness with work positioningbelt, front & rear anchorage * Titan CMS arrester with 2,5 m webbinglifeline * adjustable work positioning lanyard with 2 twist lockkarabiners1014794Titan basic Kit N°2Titan basic Kit N°2 : * 1 point TITAN Harness * 1 CS20 * Titan BasicWeb Yellow Lanyard.Titan Fall Protection Kits(EUR)Miller LogoProduct FamilyTitan fall protection kit options1014970Titan ECO KitECO KIT : * 1 point Titan Eco harness * 1 screw SC20 karabiner *1Titan basic lanyard 1,8 M lenght1013730TITAN CONSTRUCTION KIT No 1TITAN CONSTRUCTION KIT No 1 : * Titan 1 point Harness * AUTOMATICROPE GRAB 12mm ANCHORAGE Line 10M * Plastic case : 325 x 295 x105 mm1013731TITAN CONSTRUCTION KIT No 6 - 2m :TITAN CONSTRUCTION KIT No 6 - 2m : * Titan 1 point Harness * 1 Webabsorbing lanyard 2 M lenght. * Plastic case1013732TITAN CONSTRUCTION KIT No 8 - 2mTITAN CONSTRUCTION KIT No 8 - 2m1013733TITAN CONSTRUCTION KIT No 8 - 1,75mTITAN CONSTRUCTION KIT No 8 - 1,75mConstructionFall from heightKey FeaturesMiller kits are produced specifically to provide the worker with immediate selection of fall protection equipment to suit their requirements.Recommended Industries/UseHazardsWarranty InformationOur state of the art equipment provides the critical tool for worker protection, combining mobility with unsurpassed comfort.At our ISO 9001 certified manufacturing facilities in Europe, we ensure that every Miller product is made in accordance with the standards and meets your exacting requirements.90004208 lanyard EN 354, 355 (EUR)/Supplementary/Documents_and_Downloads/Fall_Protection/Lanyards/41642/1033.aspxInstruction manual90004212 harness EN 361, 358 (EUR)/Supplementary/Documents_and_Downloads/Fall_Protection/Full_Body_Harness/41640/1033.aspxInstruction manualMiller TechniservNorthern and Central EuropePhone: +49 (0) 9281/8302-205Email:*********************************Southern Europe, UK, IrelandPhone: +33 (0)2 48 52 40 42Email:****************************© Honeywell International Inc.。
SCDS127A产品说明书
SN74CBT3306CDUAL FET BUS SWITCH5ĆV BUS SWITCH WITH ć2ĆV UNDERSHOOT PROTECTIONSCDS127A − SEPTEMBER 2003 − REVISED OCTOBER 2003D Undershoot Protection for Off-Isolation on A and B Ports Up To −2 VD Bidirectional Data Flow, With Near-Zero Propagation DelayD Low ON-State Resistance (r on )Characteristics (r on = 3 Ω Typical)D Low Input/Output Capacitance Minimizes Loading and Signal Distortion (C io(OFF) = 5 pF Typical)D Data and Control Inputs Provide Undershoot Clamp Diodes D Low Power Consumption (I CC = 3 µA Max)D V CC Operating Range From 4 V to 5.5 V DData I/Os Support 0 to 5-V Signaling Levels (0.8-V, 1.2-V, 1.5-V, 1.8-V, 2.5-V, 3.3-V, 5-V)D Control Inputs Can Be Driven by TTL or 5-V/3.3-V CMOS OutputsD I off Supports Partial-Power-Down Mode OperationD Latch-Up Performance Exceeds 100 mA Per JESD 78, Class IIDESD Performance Tested Per JESD 22− 2000-V Human-Body Model (A114-B, Class II)− 1000-V Charged-Device Model (C101)DSupports Both Digital and AnalogApplications: USB Interface, Bus Isolation,Low-Distortion Signal GatingD OR PW PACKAGE(TOP VIEW)123487651OE 1A 1B GNDV CC 2OE 2B 2Adescription/ordering informationThe SN74CBT3306C is a high-speed TTL-compatible FET bus switch with low ON-state resistance (r on ),allowing for minimal propagation delay. Active Undershoot-Protection Circuitry on the A and B ports of the SN74CBT3306C provides protection for undershoot up to −2 V by sensing an undershoot event and ensuring that the switch remains in the proper OFF state.The SN74CBT3306C is organized as two 1-bit bus switches with separate output-enable (1OE, 2OE) inputs.It can be used as two 1-bit bus switches or as one 2-bit bus switch. When OE is low, the associated 1-bit bus switch is ON, and the A port is connected to the B port, allowing bidirectional data flow between ports. When OE is high, the associated 1-bit bus switch is OFF, and the high-impedance state exists between the A and B ports.ORDERING INFORMATIONT APACKAGE †ORDERABLE PART NUMBER TOP-SIDE MARKING Tube SN74CBT3306CD SOIC − DTape and reel SN74CBT3306CDR CU306C −40°C to 85°CTube SN74CBT3306CPW TSSOP − PWTape and reelSN74CBT3306CPWRCU306C†Package drawings, standard packing quantities, thermal data, symbolization, and PCB design guidelines are available at /sc/package.Copyright 2003, Texas Instruments IncorporatedPlease be aware that an important notice concerning availability, standard warranty, and use in critical applications of Texas Instruments semiconductor products and disclaimers thereto appears at the end of this data sheet.PRODUCTION DATA information is current as of publication date.SN74CBT3306CDUAL FET BUS SWITCH5ĆV BUS SWITCH WITH ć2ĆV UNDERSHOOT PROTECTIONSCDS127A − SEPTEMBER 2003 − REVISED OCTOBER 2003description/ordering information (continued)This device is fully specified for partial-power-down applications using I off . The I off feature ensures that damaging current will not backflow through the device when it is powered down. The device has isolation during power off.To ensure the high-impedance state during power up or power down, OE should be tied to V CC through a pullup resistor; the minimum value of the resistor is determined by the current-sinking capability of the driver.FUNCTION TABLE (each bus switch)INPUT INPUT/OUTPUTOE AFUNCTION L B A port = B port HZDisconnectlogic diagram (positive logic)1A 1OESW1B2A 2OESW2B215736simplified schematic, each FET switch (SW)AEN †B†EN is the internal enable signal applied to the switch.Undershoot Protection CircuitSN74CBT3306CDUAL FET BUS SWITCH5ĆV BUS SWITCH WITH ć2ĆV UNDERSHOOT PROTECTIONSCDS127A − SEPTEMBER 2003 − REVISED OCTOBER 2003 absolute maximum ratings over operating free-air temperature range (unless otherwise noted)†. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Supply voltage range, V CC−0.5 V to 7 V Control input voltage range, V IN (see Notes 1 and 2) −0.5 V to 7 V. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Switch I/O voltage range, V I/O (see Notes 1, 2, and 3) −0.5 V to 7 V. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Control input clamp current, I IK(V IN< 0) −50 mA. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .I/O port clamp current, I I/OK(V I/O< 0) −50 mA ON-state switch current, I I/O (see Note 4) ±128 mA. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Continuous current through V CC or GND terminals ±100 mA. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Package thermal impedance, θJA (see Note 5):D package 97°C/W. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .PW package 149°C/W Storage temperature range, T stg −65°C to 150°C. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .†Stresses beyond those listed under “absolute maximum ratings” may cause permanent damage to the device. These are stress ratings only, and functional operation of the device at these or any other conditions beyond those indicated under “recommended operating conditions” is not implied. Exposure to absolute-maximum-rated conditions for extended periods may affect device reliability.NOTES: 1.All voltages are with respect to ground unless otherwise specified.2.The input and output voltage ratings may be exceeded if the input and output clamp-current ratings are observed.3.V I and V O are used to denote specific conditions for V I/O.4.I I and I O are used to denote specific conditions for I I/O.5.The package thermal impedance is calculated in accordance with JESD 51-7.recommended operating conditions (see Note 6)MIN MAX UNITV CC Supply voltage4 5.5VV IH High-level control input voltage2 5.5VV IL Low-level control input voltage00.8VV I/O Data input/output voltage0 5.5VT A Operating free-air temperature−4085°C NOTE 6:All unused control inputs of the device must be held at V CC or GND to ensure proper device operation. Refer to the TI application report, Implications of Slow or Floating CMOS Inputs, literature number SCBA004.SN74CBT3306CDUAL FET BUS SWITCH5ĆV BUS SWITCH WITH ć2ĆV UNDERSHOOT PROTECTIONSCDS127A − SEPTEMBER 2003 − REVISED OCTOBER 2003electrical characteristics over recommended operating free-air temperature range (unless otherwise noted)PARAMETER TEST CONDITIONS MINTYP †MAX UNIT V IK Control inputs V CC = 4.5 V,I IN = −18 mA−1.8V V IKU Data inputs V CC = 5 V,0 mA > I I ≥ −50 mA,V IN = V CC or GND,Switch OFF−2V I IN Control inputsV CC = 5.5 V,V IN = V CC or GND ±1µA I OZ ‡V CC = 5.5 V,V O = 0 to 5.5 V,V I = 0,Switch OFF,V IN = V CC or GND ±10µA I off V CC = 0,V O = 0 to 5.5 V,V I = 010µA I CC V CC = 5.5 V,I I/O = 0,V IN = V CC or GND,Switch ON or OFF3µA ∆I CC §Control inputs V CC = 5.5 V,One input at 3.4 V,Other inputs at V CC or GND2.5mA C in Control inputs V IN = 3 V or 0 3.5pF C io(OFF)V I/O = 3 V or 0,Switch OFF,V IN = V CC or GND 5pF C io(ON)V I/O = 3 V or 0,Switch ON,V IN = V CC or GND 12.5pFV CC = 4 V,TYP at V CC = 4 VV I = 2.4 V,I O = −15 mA 812on ¶V I O = 64 mA 36r V I = 0I O = 30 mA36ΩCC = 4.5 VV I = 2.4 V,I O = −15 mA 510V IN and I IN refer to control inputs. V I , V O , I I , and I O refer to data pins.†All typical values are at V CC = 5 V (unless otherwise noted), T A = 25°C.‡For I/O ports, the parameter I OZ includes the input leakage current.§This is the increase in supply current for each input that is at the specified voltage level, rather than V CC or GND.¶Measured by the voltage drop between the A and B terminals at the indicated current through the switch. ON-state resistance is determined by the lower of the voltages of the two (A or B) terminals.switching characteristics over recommended operating free-air temperature range (unless otherwise noted) (see Figure 3)FROM TO V CC = 4 V V CC = 5 V ± 0.5 V PARAMETER(INPUT)(OUTPUT)MINMAX MINMAX UNITt pd # A or B B or A 0.240.15ns t en OE A or B 4.6 1.5 4.2ns t disOEA or B4.31.5 4.3ns #The propagation delay is the calculated RC time constant of the typical ON-state resistance of the switch and the specified load capacitance,when driven by an ideal voltage source (zero output impedance).SN74CBT3306CDUAL FET BUS SWITCH5ĆV BUS SWITCH WITH ć2ĆV UNDERSHOOT PROTECTIONSCDS127A − SEPTEMBER 2003 − REVISED OCTOBER 2003undershoot characteristics (see Figures 1 and 2)PARAMETERTEST CONDITIONSMIN TYP †MAXUNIT V OUTU V CC = 5.5 V,Switch OFF,V IN = V CC or GND2V OH −0.3V†All typical values are at V CC = 5 V (unless otherwise noted), T A = 25°C.Figure 1. Device Test Setup50 ΩV SV CC11 V 100 k Ω100 k Ω10 pFDUTInputGeneratorAxBxFigure 2. Transient Input Voltage (V I ) and OutputVoltage (V OUTU ) Waveforms(Switch OFF)−2 V5.5 V10 %20 ns10 %90 %90 %2 ns2 nsV OH − 0.3V OHOutput (V OUTU )Input (Open Socket)SN74CBT3306CDUAL FET BUS SWITCH5ĆV BUS SWITCH WITH ć2ĆV UNDERSHOOT PROTECTIONSCDS127A − SEPTEMBER 2003 − REVISED OCTOBER 2003PARAMETER MEASUREMENT INFORMATIONV OH V OLC L(see Note A)TEST CIRCUITS17 VOpen GNDR LR Lt PLH t PHLOutput Waveform 1S1 at 7 V (see Note B)Output Waveform 2S1 at Open (see Note B)t PZLt PZHt PLZt PHZ 3 V0 V V OH V OL0 VV OL + V ∆V OH − V ∆0 VOutput Control (V IN )3 V3.5 VVOLTAGE WAVEFORMSPROPAGATION DELAY TIMES (t pd(s))VOLTAGE WAVEFORMS ENABLE AND DISABLE TIMESOutputNOTES: A.C L includes probe and jig capacitance.B.Waveform 1 is for an output with internal conditions such that the output is low except when disabled by the output control.Waveform 2 is for an output with internal conditions such that the output is high except when disabled by the output control.C.All input pulses are supplied by generators having the following characteristics: PRR ≤10 MHz, Z O = 50 Ω, t r ≤2.5 ns, t f ≤2.5 ns.D.The outputs are measured one at a time with one transition per measurement.E.t PLZ and t PHZ are the same as t dis .F.t PZL and t PZH are the same as t en .G.t PLH and t PHL are the same as t pd(s). The tpd propagation delay is the calculated RC time constant of the typical ON-stateresistance of the switch and the specified load capacitance, when driven by an ideal voltage source (zero output impedance).H.All parameters and waveforms are not applicable to all devices.1.5 V1.5 V1.5 V1.5 V1.5 V 1.5 V1.5 V1.5 V50 ΩV G1V CCDUT50 ΩV IN50 ΩV G250 ΩV ITEST R L S1V ∆C L 5 V ±0.5 V4 V V CC V I t PHZ /t PZHt PLZ /t PZL t pd(s) 5 V ±0.5 V4 V5 V ±0.5 V4 VOpen Open 7 V 7 V Open Open500 Ω500 Ω500 Ω500 Ω500 Ω500 ΩV CC or GND V CC or GNDGND GND V CC V CC 50 pF 50 pF 50 pF 50 pF 50 pF 50 pF0.3 V 0.3 V 0.3 V 0.3 VOutput Control (V IN )Input GeneratorInput GeneratorV OFigure 3. Test Circuit and Voltage WaveformsPACKAGING INFORMATION(1) The marketing status values are defined as follows:ACTIVE: Product device recommended for new designs.LIFEBUY: TI has announced that the device will be discontinued, and a lifetime-buy period is in effect.NRND: Not recommended for new designs. Device is in production to support existing customers, but TI does not recommend using this part in a new design.PREVIEW: Device has been announced but is not in production. Samples may or may not be available.OBSOLETE: TI has discontinued the production of the device.(2) RoHS: TI defines "RoHS" to mean semiconductor products that are compliant with the current EU RoHS requirements for all 10 RoHS substances, including the requirement that RoHS substance do not exceed 0.1% by weight in homogeneous materials. Where designed to be soldered at high temperatures, "RoHS" products are suitable for use in specified lead-free processes. TI may reference these types of products as "Pb-Free".RoHS Exempt: TI defines "RoHS Exempt" to mean products that contain lead but are compliant with EU RoHS pursuant to a specific EU RoHS exemption.Green: TI defines "Green" to mean the content of Chlorine (Cl) and Bromine (Br) based flame retardants meet JS709B low halogen requirements of <=1000ppm threshold. Antimony trioxide based flame retardants must also meet the <=1000ppm threshold requirement.(3) MSL, Peak Temp. - The Moisture Sensitivity Level rating according to the JEDEC industry standard classifications, and peak solder temperature.(4) There may be additional marking, which relates to the logo, the lot trace code information, or the environmental category on the device.(5) Multiple Device Markings will be inside parentheses. Only one Device Marking contained in parentheses and separated by a "~" will appear on a device. If a line is indented then it is a continuation of the previous line and the two combined represent the entire Device Marking for that device.(6) Lead finish/Ball material - Orderable Devices may have multiple material finish options. Finish options are separated by a vertical ruled line. Lead finish/Ball material values may wrap to two lines if the finish value exceeds the maximum column width.Important Information and Disclaimer:The information provided on this page represents TI's knowledge and belief as of the date that it is provided. TI bases its knowledge and belief on information provided by third parties, and makes no representation or warranty as to the accuracy of such information. Efforts are underway to better integrate information from third parties. TI has taken andAddendum-Page 1continues to take reasonable steps to provide representative and accurate information but may not have conducted destructive testing or chemical analysis on incoming materials and chemicals. TI and TI suppliers consider certain information to be proprietary, and thus CAS numbers and other limited information may not be available for release.In no event shall TI's liability arising out of such information exceed the total purchase price of the TI part(s) at issue in this document sold by TI to Customer on an annual basis.Addendum-Page 2TAPE AND REEL INFORMATION*All dimensions are nominalDevicePackage Type Package Drawing Pins SPQReel Diameter (mm)Reel Width W1(mm)A0(mm)B0(mm)K0(mm)P1(mm)W (mm)Pin1Quadrant SN74CBT3306CDR SOIC D 82500330.012.4 6.4 5.2 2.18.012.0Q1SN74CBT3306CPWR TSSOP PW 82000330.012.47.0 3.6 1.68.012.0Q1SN74CBT3306CPWRTSSOPPW82000330.012.47.03.61.68.012.0Q1*All dimensions are nominalDevice Package Type Package Drawing Pins SPQ Length(mm)Width(mm)Height(mm) SN74CBT3306CDR SOIC D8*******.5336.125.0 SN74CBT3306CPWR TSSOP PW82000367.0367.035.0SN74CBT3306CPWR TSSOP PW82000364.0364.027.0TUBE*All dimensions are nominalDevice Package NamePackage TypePins SPQ L (mm)W (mm)T (µm)B (mm)SN74CBT3306CD D SOIC 87550783940 4.32SN74CBT3306CPWPWTSSOP815053010.236003.5PACKAGE MATERIALS INFORMATION5-Jan-2022PACKAGE OUTLINESOIC - 1.75 mm max heightD0008ASMALL OUTLINE INTEGRATED CIRCUITNOTES:1. Linear dimensions are in inches [millimeters]. Dimensions in parenthesis are for reference only. Controlling dimensions are in inches. Dimensioning and tolerancing per ASME Y14.5M.2. This drawing is subject to change without notice.3. This dimension does not include mold flash, protrusions, or gate burrs. Mold flash, protrusions, or gate burrs shall not exceed .006 [0.15] per side.4. This dimension does not include interlead flash.5. Reference JEDEC registration MS-012, variation AA.EXAMPLE BOARD LAYOUTSOIC - 1.75 mm max heightD0008ASMALL OUTLINE INTEGRATED CIRCUITNOTES: (continued)6. Publication IPC-7351 may have alternate designs.7. Solder mask tolerances between and around signal pads can vary based on board fabrication site.EXAMPLE STENCIL DESIGNSOIC - 1.75 mm max heightD0008ASMALL OUTLINE INTEGRATED CIRCUITNOTES: (continued)8. Laser cutting apertures with trapezoidal walls and rounded corners may offer better paste release. IPC-7525 may have alternate design recommendations.9. Board assembly site may have different recommendations for stencil design.PACKAGE OUTLINETSSOP - 1.2 mm max heightPW0008ASMALL OUTLINE PACKAGENOTES:1. All linear dimensions are in millimeters. Any dimensions in parenthesis are for reference only. Dimensioning and tolerancing per ASME Y14.5M.2. This drawing is subject to change without notice.3. This dimension does not include mold flash, protrusions, or gate burrs. Mold flash, protrusions, or gate burrs shall not exceed 0.15 mm per side.4. This dimension does not include interlead flash. Interlead flash shall not exceed 0.25 mm per side.5. Reference JEDEC registration MO-153, variation AA.EXAMPLE BOARD LAYOUT PW0008ATSSOP - 1.2 mm max heightSMALL OUTLINE PACKAGE ArrayNOTES: (continued)6. Publication IPC-7351 may have alternate designs.7. Solder mask tolerances between and around signal pads can vary based on board fabrication site.EXAMPLE STENCIL DESIGN PW0008ATSSOP - 1.2 mm max heightSMALL OUTLINE PACKAGE ArrayNOTES: (continued)8. Laser cutting apertures with trapezoidal walls and rounded corners may offer better paste release. IPC-7525 may have alternatedesign recommendations.9. Board assembly site may have different recommendations for stencil design.IMPORTANT NOTICE AND DISCLAIMERTI PROVIDES TECHNICAL AND RELIABILITY DATA (INCLUDING DATA SHEETS), DESIGN RESOURCES (INCLUDING REFERENCE DESIGNS), APPLICATION OR OTHER DESIGN ADVICE, WEB TOOLS, SAFETY INFORMATION, AND OTHER RESOURCES “AS IS” AND WITH ALL FAULTS, AND DISCLAIMS ALL WARRANTIES, EXPRESS AND IMPLIED, INCLUDING WITHOUT LIMITATION ANY IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE OR NON-INFRINGEMENT OF THIRD PARTY INTELLECTUAL PROPERTY RIGHTS.These resources are intended for skilled developers designing with TI products. You are solely responsible for (1) selecting the appropriate TI products for your application, (2) designing, validating and testing your application, and (3) ensuring your application meets applicable standards, and any other safety, security, regulatory or other requirements.These resources are subject to change without notice. TI grants you permission to use these resources only for development of an application that uses the TI products described in the resource. Other reproduction and display of these resources is prohibited. No license is granted to any other TI intellectual property right or to any third party intellectual property right. TI disclaims responsibility for, and you will fully indemnify TI and its representatives against, any claims, damages, costs, losses, and liabilities arising out of your use of these resources.TI’s products are provided subject to TI’s Terms of Sale or other applicable terms available either on or provided in conjunction with such TI products. TI’s provision of these resources does not expand or otherwise alter TI’s applicable warranties or warranty disclaimers for TI products.TI objects to and rejects any additional or different terms you may have proposed.Mailing Address: Texas Instruments, Post Office Box 655303, Dallas, Texas 75265Copyright © 2022, Texas Instruments Incorporated。
loss of control inflight Accident list (1996-2010)
N-240CJ N-195GA N-112AX AP-BJD N-117WM N-81PF N-899RW N-648KA N-925TT N-299GL C-FDYF CC-CFM N-206AV N-437RA N-511AT N-113SH N-501EZ N-405PC N-77215 D-CASH N-550BP OK-ACH HB-VLV N-500AT N-391QS I-FEEV C-FSKI N-958CA N-90AG N-8396A B-3072 N-873G N-699CW N-900LG N-322FX N-168CK N-470ZW EW-101PJ ZK-KFU SX-ECH N-523AC N-68043 N-75142 ZS-NKK C-FNTF N-54AA YV-224C N-313RS N-27UA N-8079U N-825BX
12/21/2002 11/4/2010 10/22/1996 10/21/2009 11/7/1996 6/9/1996 5/8/1997 4/7/1999 8/31/1999 9/2/1999 7/17/2000 3/6/2003 7/8/2003 1/3/2004 8/11/2004 8/14/2005 9/5/200529/2006 10/29/2006 1/1/2007 5/5/2007 9/23/2007 9/14/2008 2/25/2009 1/25/2010 2/13/2010 7/17/1996 11/12/1996 12/22/1999 2/27/2000 5/25/2002 10/9/2002 ZOUTU 5/11/2009 9/3/2010 2/6/1996 10/2/1996 1/22/2002 7/1/2002 10/20/2002 1/28/2009 3/30/2000 8/1/2005 5/4/2002 4/19/1997 7/30/1998 9/14/2001 1/8/2003
Leviton ATLAS-X1 Cat 6A Component-Rated UTP QUICKP
Page 1 of 2APPLICATIONThe ATLAS-X1 Cat 6A Component-Rated UTP QUICKPORT Jack supports 10GBASE-T networks. The jack is part of a complete ATLAS-X1 Cat 6A UTP system, ideal for the most demanding mission-critical network applications. The connector supports emerging technologies and will easily adapt to network trends.SPECIFICATIONThe jack shall meet or exceed the requirements for channel and component-level electrical transmission performance as described in ANSI/TIA-568.2-D (Cat 6A), ISO/IEC11801-1 (Class E A ), and EN 50173-1 (Class E A ). The jack shall be compliant with ANSI/TIA-1096-A, c(UL)us Listed, and be independently verified for electrical transmission performance and power delivery. The jack body shall be made of die-cast zinc and all plastic components shall be made of high-impact, fire-retardant plastic rated UL 94V-0. The jack shall support tool-free termination and re-termination and shall not require a specialized termination tool. The jack wiring shall be universal to accommodate T568A and T568B wiring schemes. The jack shall be available in 13 colors; more than established by the ANSI/TIA-606-C standard. The jack shall be offered in standard and shuttered styles and select jacks shall be supplied with interchangeable icons. The jack shall be compliant with IEEE 802.3 PoE Type 1, 2, 3, 4 (100 watts max).DESIGN CONSIDERATIONS• Use in any QUICKPORT™ housing to support Cat 6A UTP connectivity in surface-mount, flush-mount, or modular furniture outlets and field-configurable panels• Can be used in conjunction with other QUICKPORT snap-in modules for voice/data/video applications over UTP , coax, and fiber• To identify ports, use different colored modules andicons for each application (full selection of ANSI/TIA-606 compatible colors, 13 available)• Robust housing and shutter protects the jack in harsh environmentsATLAS-X1™ Cat 6A Component-Rated UTP QUICKPORT™ Jack6AUJK-xx6, ICONS-ICxFEATURES• Independently tested and guaranteed to exceed all component, permanent link, and channel margins• Patented Retention Force Technology™ (RFT) protects against tine damage and increases system longevity • For Power over Ethernet, RFT maintains contact force between plug and jack, preventing arcing from intermittent disconnects• Unique design supports tool-free termination andre-termination and requires no specialized termination tool • Short jack design supports a wider range of applications (e.g. shallow boxes, enclosures, bend radius, etc.)• Terminates from 26 to 22 AWG solid or stranded conductors for use on various cable types• Robust IDCs can withstand 20 re-termination cycles and jack contacts are tested for 750 plug-mating cycles to ensure system longevity• Available in 13 ANSI/TIA-606-C compatible colors • Tested and approved for use in air-handling spaces (plenum rating) in accordance with UL Standard 2043• Select jacks available with interchangeable icons (voice, data, A/V, blank) for easy ID• Jack with internal shutter protects against dust and debris • Solid metal body dissipates 53% more heat than plastic, minimizing damage from excess heat in PoE applications • Tine geometry prevents arcing damage where plug and jack make contactSTANDARDS & REGULATIONS• ANSI/TIA-568.2-D (Cat 6A)• ISO/IEC 11801-1 (Cat 6A)• EN 50173-1 (Cat 6A)• ANSI/TIA-1096-A (formerly FCC Part 68)• IEC 60603-7 (includes IEC 60512-5-2)• IEC 60512-99-002• IEEE 802.3 PoE Type 1, 2, 3, 4 (100 watts max)• Cisco UPOE, UPOE+ (90 watts max)• Power over HDBaseT™ PoH (95 watts max)• c(UL)us Listed (UL 1863)• UL 2043 Plenum Certified • RoHS 3• ETL verified to meet the IEC 60512-99-002 standard for support of IEEE 802.3 Type 4 PoE (100 watt) applicationsCOUNTRY OF ORIGINUSA and Mexico (Contact Customer Service for details)6AUJK-xx6, ICONS-ICxUSANetwork Solutions Headquarters +1 (800) 722 2082 *******************Leviton Berk-Tek Cable : +1 (800) 237 5835 ************************Asia Pacific+852 3620 2602********************Canada+1 (800) 461 2002**********************Europe+44 (0) 1592 772124 **********************Latin AmericaMX: +52 (55) 2128 6286 LATAM: +52 (55) 2333 5963 *********************Middle East & Africa +971 (4) 247 9800 *******************NETWORK SOLUTIONS PRODUCTS ARE AVAILABLE WORLDWIDE IN OVER 100 COUNTRIES. VISIT US ONLINE AT /NS TO LEARN MORE.Page 2 of 2For further support information, visit /ns/support6AUJK-xx6, ICONS-ICx6AUJK-xx6, ICONS-ICxMECHANICAL SPECIFICATIONSDimensions:See belowMaterials: Jack Body: Die-cast zincSpring-Wire Contacts: High quality, copper-based alloy, plated with 50 microinches of gold for lowest contact resistance andmaximum life Temp. (Storage):Temp. (Installation): Temp. (Operating):Humidity (Max.):WARRANTY INFORMATIONFor Leviton product warranties, go to /ns/warrantyPART NUMBERDescriptionStandard Jack Jack with Shutter GREENPACK™12-Pack Standard Jack ATLAS-X1™ Cat 6A Component-Rated UTP QUICKPORT™ Jack, white 6AUJK-RW66AUJK-SW66AUJK-CW6ATLAS-X1 Cat 6A Component-Rated UTP QUICKPORT Jack, light almond 6AUJK-RT66AUJK-ST6—ATLAS-X1 Cat 6A Component-Rated UTP QUICKPORT Jack, ivory 6AUJK-RI66AUJK-SI6—ATLAS-X1 Cat 6A Component-Rated UTP QUICKPORT Jack, yellow 6AUJK-RY66AUJK-SY6—ATLAS-X1 Cat 6A Component-Rated UTP QUICKPORT Jack, orange 6AUJK-RO66AUJK-SO6—ATLAS-X1 Cat 6A Component-Rated UTP QUICKPORT Jack, crimson 6AUJK-RC66AUJK-SC6—ATLAS-X1 Cat 6A Component-Rated UTP QUICKPORT Jack, dark red 6AUJK-RR66AUJK-SR6—ATLAS-X1 Cat 6A Component-Rated UTP QUICKPORT Jack, purple 6AUJK-RP66AUJK-SP6—ATLAS-X1 Cat 6A Component-Rated UTP QUICKPORT Jack, blue 6AUJK-RL66AUJK-SL66AUJK-CL6ATLAS-X1 Cat 6A Component-Rated UTP QUICKPORT Jack, green 6AUJK-RV66AUJK-SV6—ATLAS-X1 Cat 6A Component-Rated UTP QUICKPORT Jack, gray 6AUJK-RG66AUJK-SG6—ATLAS-X1 Cat 6A Component-Rated UTP QUICKPORT Jack, black6AUJK-RE66AUJK-SE66AUJK-CE6ATLAS-X1 Cat 6A Component-Rated UTP QUICKPORT Jack, brown6AUJK-RB66AUJK-SB6—Green (V)Blue (L)Purple (P)Crimson (C)Dark Red (R)Orange (O)Yellow (Y)Black (E)Gray (G)Ivory (I)Light Almond (T)Brown (B)Color-matched icons (ICONS-ICx) can be ordered separately in 72-quantity packs.x = icon color。
阿特拉斯枪样本
电动装配工具和系统
69
工具代码............................................................................. 70 产品安全............................................................................. 74 工业用电池螺丝刀............................................................... 75 电动螺丝刀 – Eliza, EBL..................................................... 84 电动螺丝刀 – 电动微扭螺丝刀MicroTorqueTM..................... 87 电动螺丝刀 – Tensor DL..................................................... 90 电动螺丝刀 – Tensor SL..................................................... 91 电动拧紧扳手 – Tensor DS................................................. 92 电动拧紧扳手 – Tensor S.................................................... 98 电动拧紧扳手 – Tensor ST............................................... 103 Tensor DS / DL控制器.......................................................111 Power Focus.................................................................... 112 Power Focus / ETX拧紧轴和电缆..................................... 130 Power MACS 4000 / QST拧紧轴和电缆........................... 133 Power MACS / QMX拧紧轴和电缆................................... 141 刀头和动力套筒................................................................ 149 扭力臂............................................................................... 156
DS-2XS6A46G1 P-IZS C36S80 4 MP ANPR 自动 Number Plat
DS-2XS6A46G1/P-IZS/C36S804 MP ANPR Bullet Solar Power 4G Network Camera KitIt can be used in the areas that are not suitable for laying wired network and electric supply lines, or used for the scenes that feature tough environment and have high demanding for device stability. It can be used for monitoring the farms, electric power cables, water and river system, oil pipelines and key forest areas.It also can be used in the temporary monitoring scenes, such as the large-scale competitions, the sudden public activity, the temporary traffic control and the city construction.Empowered by deep learning algorithms, Hikvision AcuSense technology brings human and vehicle targets classification alarms to front- and back-end devices. The system focuses on human and vehicle targets, vastly improving alarm efficiency and effectiveness.⏹ 80 W photovoltaic panel, 360 Wh chargeable lithium battery⏹ Clear imaging against strong back light due to 120 dB trueWDR technology⏹ Focus on human and vehicle targets classification based ondeep learning⏹Support battery management, battery display, batteryhigh-low temperature protection, charge-dischargeprotection, low-battery sleep protection and remotewakeup ⏹ LTE-TDD/LTE-FDD/WCDMA/GSM 4G wireless networktransmission, support Micro SIM card⏹Water and dust resistant (IP66) *The Wi-Fi module of this product only supports AP mode on Channel 11, and does not support other modes and channels.FunctionRoad Traffic and Vehicle DetectionWith embedded deep learning based license plate capture and recognition algorithms, the camera alone can achieve plate capture and recognition. The algorithm enjoys the high recognition accuracy of common plates and complex-structured plates, which is a great step forward comparing to traditional algorithms. Blocklist and allowlist are available for plate categorization and separate alarm triggering.SpecificationCameraImage Sensor 1/1.8" Progressive Scan CMOSMax. Resolution 2560 × 1440Min. Illumination Color: 0.0005 Lux @ (F1.2, AGC ON), B/W: 0 Lux with light Shutter Time 1 s to 1/100,000 sLensLens Type Auto, Semi-auto, ManualFocal Length & FOV 2.8 to 12 mm, horizontal FOV 107.4° to 39.8°, vertical FOV 56° to 22.4°, diagonal FOV 130.1° to 45.7°8 to 32 mm, horizontal FOV 40.3° to 14.5°, vertical FOV 22.1° to 8.2°, diagonal FOV 46.9° to 16.5°Iris Type Auto-irisLens Mount All In One LensAperture 2.8 to 12 mm: F1.2, 8 to 32 mm: F1.6 DORIDORI 2.8 to 12 mm:Wide: D: 60.0 m, O: 23.8 m, R: 12.0 m, I: 6.0 m Tele: D: 149.0 m, O: 59.1 m, R: 29.8 m, I: 14.9 m 8 to 32 mm:Wide: D: 150.3 m, O: 59.7 m, R: 30.1 m, I: 15.0 m Tele: D: 400 m, O: 158.7 m, R: 80 m, I: 40 mIlluminatorSupplement Light Type IRSupplement Light Range 2.8 to 12 mm: Up to 30 m 8 to 32 mm: Up to 50 mSmart Supplement Light Yes VideoMain Stream Performance mode:50 Hz: 25 fps (2560 × 1440, 1920 × 1080, 1280 × 720) 60 Hz: 30 fps (2560 × 1440, 1920 × 1080, 1280 × 720) Proactive mode:50 Hz: 12.5 fps (2560 × 1440, 1920 × 1080, 1280 × 720) 60 Hz: 15 fps (2560 × 1440, 1920 × 1080, 1280 × 720)Sub-Stream Performance mode:50 Hz: 25 fps (640 × 480, 640 × 360) 60 Hz: 30 fps (640 × 480, 640 × 360) Proactive mode:50 Hz: 12.5 fps (640 × 480, 640 × 360) 60 Hz: 15 fps (640 × 480, 640 × 360)Third Stream 50 Hz: 1 fps (1280 × 720, 640 × 480) 60 Hz: 1 fps (1280 × 720, 640 × 480)Video Compression Main stream: H.264/H.265Sub-stream: H.264/H.265/MJPEGThird Stream: H.265/H.264*Performance mode: main stream supports H.264+, H.265+Video Bit Rate 32 Kbps to 8 MbpsH.264 Type Baseline Profile, Main Profile, High ProfileH.265 Type Main ProfileBit Rate Control CBR/VBRScalable Video Coding (SVC) H.264 and H.265 encodingRegion of Interest (ROI) 4 fixed regions for main streamAudioAudio Compression G.711/G.722.1/G.726/MP2L2/PCM/MP3/AAC-LCAudio Bit Rate 64 Kbps (G.711ulaw/G.711alaw)/16 Kbps (G.722.1)/16 Kbps (G.726)/32 to 192 Kbps (MP2L2)/8 to 320 Kbps (MP3)/16 to 64 Kbps (AAC-LC)Audio Sampling Rate 8 kHz/16 kHz/32 kHz/44.1 kHz/48 kHzEnvironment Noise Filtering YesNetworkSimultaneous Live View Up to 6 channelsAPI Open Network Video Interface (Profile S, Profile G, Profile T), ISAPI, SDK, ISUP, OTAPProtocols TCP/IP, ICMP, HTTP, HTTPS, FTP, DHCP, DNS, DDNS, RTP, RTSP, RTCP, NTP, UPnP, SMTP, SNMP, IGMP, 802.1X, QoS, IPv6, UDP, Bonjour, SSL/TLS, WebSocket, WebSocketsUser/Host Up to 32 users3 user levels: administrator, operator, and userSecurity Password protection, complicated password, HTTPS encryption, 802.1X authentication (EAP-TLS, EAP-LEAP, EAP-MD5), watermark, IP address filter, basic and digest authentication for HTTP/HTTPS, WSSE and digest authentication for Open Network Video Interface, RTP/RTSP over HTTPS, control timeout settings, TLS 1.2, TLS 1.3Network Storage NAS (NFS, SMB/CIFS), auto network replenishment (ANR)Together with high-end Hikvision memory card, memory card encryption and health detection are supported.Client Hik-Connect (proactive mode also supports), Hik-central ProfessionalWeb Browser Plug-in required live view: IE 10+Plug-in free live view: Chrome 57.0+, Firefox 52.0+ Local service: Chrome 57.0+, Firefox 52.0+Mobile CommunicationSIM Card Type MicroSIMFrequency LTE-TDD: Band38/40/41LTE-FDD: Band1/3/5/7/8/20/28 WCDMA: Band1/5/8GSM: 850/900/1800 MHzStandard LTE-TDD/LTE-FDD/WCDMA/GSM ImageWide Dynamic Range (WDR) 120 dBDay/Night Switch Day, Night, Auto, Schedule, Video Trigger Image Enhancement BLC, HLC, 3D DNR, DefogImage Parameters Switch YesImage Settings Saturation, brightness, contrast, sharpness, gain, white balance, adjustable by client software or web browserSNR ≥ 52 dBPrivacy Mask 4 programmable polygon privacy masks InterfaceAudio 1 input (line in), max. input amplitude: 3.3 Vpp, input impedance: 4.7 KΩ, interface type: non-equilibrium,1 output (line out), max. output amplitude: 3.3 Vpp, output impedance: 100 Ω, interface type: non-equilibriumAlarm 1 input, 1 output (max. 12 VDC, 1 A)On-Board Storage Built-in memory card slot, support microSD card, up to 256 GB, Built-in 8 GB eMMC storageReset Key YesEthernet Interface 1 RJ45 10 M/100 M self-adaptive Ethernet portWiegand 1 Wiegand (CardID 26bit, SHA-1 26bit, Hik 34bit, NEWG 72 bit) EventBasic Event Motion detection, video tampering alarm, exception (network disconnected, IP address conflict, illegal login, HDD error)Smart Event Line crossing detection, intrusion detection, region entrance detection, region exiting detection, unattended baggage detection, object removal detection, scene change detection, face detectionLinkage Upload to FTP/NAS/memory card, notify surveillance center, send email, trigger recording, trigger capture, trigger alarm output, audible warningDeep Learning FunctionRoad Traffic and Vehicle Detection Blocklist and allowlist: up to 10,000 records Support license plate recognition License plate recognition rate ≥95%GeneralPower 12 VDC ± 20%, 4-pin M8 waterproof connector1. Standby power consumption: 45 mW2. The average power consumption of 24 hours:3.5 W (4G transmission is excluded).3. The max. power consumption: 7 WMaterial Front cover: metal, body: metal, bracket: metalDimension 816.2 mm × 735.9 mm × 760 mm (32.1" × 28.9" × 29.9") (Max. size of the camera after it is completely assembled)Package Dimension 862 mm × 352 mm × 762 mm (33.9" × 13.9" × 30.0")Weight Approx. 31.885 kg (70.3 lb.)With Package Weight Approx. 25.650 kg (56.5 lb.)Storage Conditions -20 °C to 60 °C (-4 °F to 140 °F). Humidity 95% or less (non-condensing) Startup and OperatingConditions-20 °C to 60 °C (-4 °F to 140 °F). Humidity 95% or less (non-condensing)Language 33 languages: English, Russian, Estonian, Bulgarian, Hungarian, Greek, German, Italian, Czech, Slovak, French, Polish, Dutch, Portuguese, Spanish, Romanian, Danish, Swedish, Norwegian, Finnish, Croatian, Slovenian, Serbian, Turkish, Korean, Traditional Chinese, Thai, Vietnamese, Japanese, Latvian, Lithuanian, Portuguese (Brazil), UkrainianGeneral Function Anti-banding, heartbeat, mirror, flash log, password reset via email, pixel counter BatteryBattery Type LithiumCapacity 360 Wh (90 Wh for each battery)Max. Output Voltage 12.6 V Battery Voltage 10.8 VOperating Temperature Charging: -20 °C to 45 °C (-4 °F to 113 °F) Discharging: -20 °C to 60 °C (-4 °F to 140 °F)Cycle Lifetime Performance mode: 5 days, Proactive mode: 8 days, Standby mode: 80 days *in cloudy/rainy days (25 °C)Battery Life More than 500 cyclesBattery Weight Approx. 2.74 kg (6.0 lb.) (0.685 kg (1.5 lb.) for each battery) ApprovalEMC CE-EMC/UKCA (EN 55032:2015+A11:2020+A1:2020, EN 50130-4:2011+A1:2014); RCM (AS/NZS CISPR 32: 2015);IC (ICES-003: Issue 7)RF CE-RED/UKCA (EN 301908-1, EN 301908-2, EN 301908-13, EN 301511, EN 301489-1, EN 301489-52, EN 62133);ICASA: same as CE-RED;IC ID (RSS-132 Issue 3, RSS-133 Issue 6, RSS-139 Issue 3, RSS-130 Issue 2, RSS-102 Issue 5);MIC (Article 49-6-4 and 49-6-5 the relevant articles and MIC Notice No. 1299 of the Ordinance Regulating Radio Equipment)Safety CB (IEC 62368-1:2014+A11)CE-LVD/UKCA (EN 62368-1:2014/A11:2017) LOA (IEC/EN 60950-1)Environment CE-RoHS (2011/65/EU);WEEE (2012/19/EU);Reach (Regulation (EC) No 1907/2006)Protection Camera: IP66 (IEC 60529-2013)Wind resistance 12 level, up to 40 m/s wind speed resistance⏹Typical ApplicationHikvision products are classified into three levels according to their anti-corrosion performance. Refer to the following description to choose for your using environment.This model has NO SPECIFIC PROTECTION.Level DescriptionTop-level protection Hikvision products at this level are equipped for use in areas where professional anti-corrosion protection is a must. Typical application scenarios include coastlines, docks,chemical plants, and more.Moderate protection Hikvision products at this level are equipped for use in areas with moderate anti-corrosion demands. Typical application scenarios include coastal areas about 2kilometers (1.24 miles) away from coastlines, as well as areas affected by acid rain. No specific protection Hikvision products at this level are equipped for use in areas where no specific anti-corrosion protection is needed.⏹Available ModelDS-2XS6A46G1/P-IZS/C36S80 (2.8-12mm)DS-2XS6A46G1/P-IZS/C36S80 (8-32mm)Dimension。
实验用荣昌猪SLA-1等位基因鉴定及分子遗传特征分析
畜牧兽医学报 2023,54(7):3064-3077A c t a V e t e r i n a r i a e t Z o o t e c h n i c a S i n i c ad o i :10.11843/j.i s s n .0366-6964.2023.07.037开放科学(资源服务)标识码(O S I D ):实验用荣昌猪S L A -1等位基因鉴定及分子遗传特征分析刘弘毅,罗霆宇,李昌文,于海波,路小野,陈洪岩,夏长友*,高彩霞*(中国农业科学院哈尔滨兽医研究所,动物疫病防控全国重点实验室,黑龙江省实验动物与比较医学重点实验室,国家禽类实验动物资源库,哈尔滨150069)摘 要:旨在明确实验用荣昌猪S L A 遗传背景,深入研究S L A -1分子相关的抗原递呈和免疫应答机制,本研究对27头荣昌猪采集抗凝血,分离外周血淋巴细胞,提取总R N A ,设计特异性引物对S L A -1基因进行R T -P C R 扩增㊁克隆和测序,对获得序列的分子特征进行分析㊂结果显示,荣昌猪中共获得11个S L A -1等位基因,其中,9个为新等位基因,全部获得G e n B a n k 登录号和I S A G S L A 命名委员会官方命名,S L A -1*24:01等位基因在该群体中频率最高㊂S L A -1基因编码区全长1086b p,存在127个核苷酸多态位点,非同义单核苷酸多态性位点数高于同义单核苷酸多态性位点数㊂核苷酸多样度为0.0449,单倍型多样度为1,平均核苷酸差异数为48.782,G+C 含量为64.9%㊂S L A -1基因编码的361个氨基酸中,有75个氨基酸变异位点;第2和第3外显子编码区多态性最高,且第2外显子区域的氨基酸变异程度高于第3外显子区,组成抗原肽结合槽6个口袋的33个关键氨基酸位点中,11个在人与荣昌猪之间高度保守,与β2-微球蛋白结合的19个关键氨基酸位点中,10个保持一致,C D 8分子与MH C 结合的关键氨基酸位点中,只有225(T h r /S e r )和228(T h r /M e t )位点不同㊂同源性和系统进化树分析表明,S L A -1*10:03和S L A -1*18:03分别与人H L A -A *02:01和H L A -A *11:01等位基因的同源性最高,荣昌猪与亚洲野猪㊁巴马小型猪和融水小型猪等亚洲猪种具有较近的亲缘关系㊂本研究成功获得了中国地方猪种荣昌猪S L A -1基因,发现其具有极为丰富的多态性,为揭示荣昌猪的S L A 遗传背景和开展异种移植研究奠定了遗传学基础㊂关键词:荣昌猪;S L A -1等位基因;鉴定;分子特征中图分类号:S 852.4 文献标志码:A 文章编号:0366-6964(2023)07-3064-14收稿日期:2022-11-17基金项目:国家重点研发计划项目(2021Y F F 0703000);国家自然科学基金(31872313);中央级公益性科研院所基本科研业务费专项(1610302022018);兽医生物技术国家重点实验室课题(S K L V B P 202120;S K L V B P 202101)作者简介:刘弘毅(1997-),男,湖南常德人,硕士生,主要从事实验动物免疫遗传学研究,E -m a i l :m 175********@163.c o m*通信作者:夏长友,主要从事实验动物学研究,E -m a i l :x i a c h a n g y o u @c a a s .c n ;高彩霞,主要从事实验动物免疫遗传学研究,E -m a i l :ga o c a i x i a @c a a s .c nI d e n t i f i c a t i o n o f S L A -1A l l e l e s a n d A n a l ys i s o f M o l e c u l a r G e n e t i c C h a r a c t e r i s t i c s i n R o n g c h a n g P i gs L I U H o n g y i ,L U O T i n g y u ,L I C h a n g w e n ,Y U H a i b o ,L U X i a o y e ,C H E N H o n g ya n ,X I A C h a n g yo u *,G A O C a i x i a *(S t a t e K e y L a b o r a t o r y f o r A n i m a l D i s e a s e C o n t r o l a n d P r e v e n t i o n ,H e i l o n g j i a n g P r o v i n c i a l K e y L a b o r a t o r y o f L a b o r a t o r y A n i m a l a n d C o m p a r a t i v e M e d i c i n e ,N a t i o n a l P o u l t r y L a b o r a t o r yA n i m a l R e s o u r c e C e n t e r ,H a r b i n V e t e r i n a r y R e s e a r c h I n s t i t u t e ,C h i n e s e A c a d e m y o fA gr i c u l t u r a l S c i e n c e s ,H a r b i n 150069,C h i n a )A b s t r a c t :T h e s t u d y a i m e d t o c l a r i f y t h e S L A g e n e t i c b a c k g r o u n d o f e x p e r i m e n t a l R o n g c h a n g p i gs a n d f u r t h e r s t u d y S L A -1m o l e c u l e -r e l a t e d a n t i g e n p r e s e n t a t i o n a n d i mm u n e r e s po n s e m e c h a n i s m.7期刘弘毅等:实验用荣昌猪S L A-1等位基因鉴定及分子遗传特征分析I n t h i s s t u d y,t h e a n t i c o a g u l a n t b l o o d s w e r e c o l l e c t e d f r o m27R o n g c h a n g p i g s,a n d p e r i p h e r a l b l o o d l y m p h o c y t e s w e r e i s o l a t e d.T o t a l R N A w e r e e x t r a c t e d a n d S L A-1g e n e s w e r e a m p l i f i e d u s i n g s p e c i f i c p r i m e r s w i t h R T-P C R m e t h o d,c l o n e d a n d s e q u e n c e d.M o l e c u l a r g e n e t i c c h a r a c t e-r i s t i c s o f t h e o b t a i n e d s e q u e n c e s w e r e f u r t h e r a n a l y z e d.T h e r e s u l t s s h o w e d t h a t a t o t a l o f11 S L A-1a l l e l e s w e r e o b t a i n e d i n R o n g c h a n g p i g s,o f w h i c h n i n e w e r e n o v e l a l l e l e s.A l l a l l e l e s o b-t a i n e d G e n B a n k a c c e s s i o n n u m b e r s a n d o f f i c i a l n a m e s w e r e a s s i g n e d b y t h e I S A G S L A N o m e n c l a-t u r e C o mm i t t e e.T h e f r e q u e n c y o f S L A-1*24:01a l l e l e w a s t h e h i g h e s t i n t h i s p o p u l a t i o n.T h e f u l l-l e n g t h o f t h e c o d i n g r e g i o n o f S L A-1g e n e w a s1086b p,w i t h127n u c l e o t i d e p o l y m o r p h i c s i t e s.T h e n u m b e r o f n o n-s y n o n y m o u s S N P s w a s h i g h e r t h a n t h a t o f s y n o n y m o u s S N P s.T h e n u-c l e o t i d e d i v e r s i t y w a s0.0449,t h e h a p l o t y p e d i v e r s i t y w a s1,t h e a v e r a g e n u c l e o t i d e d i f f e r e n c e n u m b e r w a s48.782,a n d t h e G+C c o n t e n t w a s64.9%.T h e r e w e r e75a m i n o a c i d v a r i a t i o n s i t e s a m o n g361a m i n o a c i d s e n c o d e d b y S L A-1g e n e.T h e c o d i n g r e g i o n o f e x o n2a n d e x o n3h a d t h e h i g h e s t p o l y m o r p h i s m,a n d t h e d e g r e e o f a m i n o a c i d v a r i a t i o n i n e x o n2r e g i o n w a s h i g h e r t h a n t h a t i n e x o n3r e g i o n.A m o n g t h e33k e y a m i n o a c i d s i t e s t h a t m a k e u p t h e s i x p o c k e t s o f t h e p e p-t i d e-b i n d i n g g r o o v e,11o f t h e m w e r e h i g h l y c o n s e r v e d b e t w e e n h u m a n a n d R o n g c h a n g p i g,a n d 10o f t h e19k e y a m i n o a c i d s i t e s w e r e c o n s i s t e n t w i t hβ2-m i c r o g l o b u l i n.O n l y t w o s i t e s,225 (T h r/S e r)a n d228(T h r/M e t),w e r e d i f f e r e n t a m o n g t h e k e y a m i n o a c i d s i t e s t h a t C D8m o l e-c u l e s b i n d t o MH C.T h e a n a l y s i s o f h o m o l o g y a n d p h y l o g e n e t i c t r e e s h o w e d t h a t S L A-1*10:03 a n d S L A-1*18:03h a d t h e h i g h e s t h o m o l o g y w i t h h u m a n H L A-A*02:01a n d H L A-A*11:01a l-l e l e s,r e s p e c t i v e l y.R o n g c h a n g p i g w a s c l o s e l y r e l a t e d t o A s i a n b o a r,B a m a m i n i a t u r e p i g,R o n g-s h u i m i n i a t u r e p i g a n d o t h e r A s i a n p i g b r e e d s.T h e S L A-1a l l e l e s o f C h i n e s e R o n g c h a n g p i g s w e r e s u c c e s s f u l l y i d e n t i f i e d,a n d i t w a s f o u n d t o h a v e h i g h l y r i c h p o l y m o r p h i s m.T h e r e s u l t s o f t h i s s t u d y p r o v i d e d a g e n e t i c f o u n d a t i o n f o r r e v e a l i n g t h e S L A g e n e t i c b a c k g r o u n d o f R o n g c h a n g p i g s a n d c o n d u c t i n g x e n o t r a n s p l a n t a t i o n r e s e a r c h.K e y w o r d s:R o n g c h a n g p i g;S L A-1a l l e l e;i d e n t i f i c a t i o n;m o l e c u l a r c h a r a c t e r i s t i c s*C o r r e s p o n d i n g a u t h o r s:X I A C h a n g y o u,E-m a i l:x i a c h a n g y o u@c a a s.c n;G A O C a i x i a,E-m a i l: g a o c a i x i a@c a a s.c n主要组织相容性复合体(m a j o r h i s t o c o m p a t i-b i l i t y c o m p l e x,MH C)是一组编码主要组织相容性抗原的基因群总称,最早在小鼠体内被发现[1-2]㊂MH C作为组织细胞的遗传标记分子,与动物的抗病性㊁抗原识别㊁递呈和免疫调节密切相关[3-5]㊂此外,MH C编码的高度多态的蛋白对器官移植成功与否具有关键作用[6-9]㊂猪MH C又称猪白细胞抗原(s w i n e l e u k o c y t e a n t i g e n,S L A),位于猪第7号染色体并横跨着丝点,是包含猪基因组中许多免疫相关基因的紧密连锁区域[10-11]㊂S L A基因包含高度多态的S L A I类㊁S L A I I类和相对保守的S L A Ⅲ类基因,高度多态的S L A-1基因是经典的S L A I 类基因,目前I P D-MH C(I mm u n o P o l y m o r p h i s m D a t a b a s e-MH C)数据库共收录了89个S L A-1基因(R e l e a s e3.9.0.107/2022b u i l d209)㊂S L A-1基因编码的S L A I类分子通过内源性抗原递呈,将胞内的抗原肽递呈到细胞表面,经C D8+T细胞识别并引起细胞免疫,对于机体的健康以及对疾病的抗病性具有重要作用㊂S L A-1包含8个外显子,第1外显子编码前导多肽链,第2~4外显子分别编码α1~α3胞外域,第5外显子编码跨膜结构域,第6~ 8外显子编码胞内域,其中,第2和第3外显子编码的α1和α2胞外域多态性最高,二者形成的抗原肽结合槽(p e p t i d e-b i n d i n g g r o o v e,P B G)决定了与之结合的T淋巴细胞表位的特异性[12]㊂有研究利用S L A-1分子体外构建S L A I类复合体研究与病毒T细胞表位结合的特异性,发现不同S L A-1分子结合T细胞表位的能力不同[13],这就提示携带不同S L A-1等位基因的猪对疫病的抵抗力不同㊂猪是世界范围内广泛培育的重要经济动物,是5603畜牧兽医学报54卷人们日常生活主要的动物蛋白来源,而且由于猪与人类在基因组㊁血液生理指标㊁解剖学结构和成分等方面高度相似,使其逐渐成为一种重要的实验动物,应用于临床器官移植㊁动物疾病模型构建和病原致病机制研究等领域[14-17]㊂荣昌猪是中国西南地区的本土猪种,由于其肉质好和抗逆性强成为中国主要的优良猪种之一,目前,已在各领域表现出重要的应用价值,如用于建立人类医学动物模型,M I T F-M基因突变形成的白化耳聋荣昌猪可以作为一种人类遗传性神经性听力缺陷的大型哺乳动物模型,在人工耳蜗应用以及研究中国耳聋遗传方面具有十分重要的意义[18-20]㊂同时荣昌猪具有较强的抗病性,已用于建立猪病原感染模型并研究自身免疫机制,为重大疫病防控提供支撑,同时也用于筛选独特的免疫相关分子,为抗病育种奠定基础㊂2019年,中国农业科学院哈尔滨兽医研究所从重庆市畜牧科学院生物工程研究所引入27头荣昌猪,屏障环境饲养繁育,主要用于培育标准化的实验猪种群,同时开展了疫病净化㊁疾病模型构建及应用研究㊂本研究就该荣昌猪种群S L A-1基因特征进行分析,旨在为标准化实验荣昌猪的培育和推广应用奠定基础,也为异种移植研究提供遗传学参考㊂1材料与方法1.1试验材料通过前腔静脉采集中国农业科学院哈尔滨兽医研究所保存的27头荣昌猪(:6,:21)的抗凝血5m L,置于E D T A抗凝管,用于分离外周血淋巴细胞,27头荣昌猪的编号如表1所示㊂1.2主要试剂猪外周血淋巴细胞分离液购自天津市灏洋生物制品科技有限公司,S i m p l y总R N A提取试剂盒购自B i o s h a r p公司,D N A胶回收试剂盒购自OM E-G A公司,O n e s t e p P C R试剂盒㊁感受态细胞大肠杆菌(E s c h e r i c h i a c o l i)D H5α和载体p M D18-T v e c t o r均购自宝生物工程(大连)有限公司㊂1.3外周血淋巴细胞的分离和R N A的提取根据猪外周血淋巴细胞分离液说明书,将猪外周血淋巴细胞分离,-80ħ保存㊂根据S i m p l y总R N A提取试剂盒说明书提取外周血淋巴细胞总R N A,利用超微量分光光度计(N a n o P h o t o m e t e r,德国)测量总R N A浓度,-80ħ保存㊂1.4引物设计与合成参考G e n B a n k中大白猪H01单倍型序列(登录号:A J251829),利用P r i m e r P r e m i e r5软件设计引物扩增S L A-1基因,扩增片段约为1537b p㊂S L A1-F:5'-C T C A G C T T C T C C C C A G A C C C C G A-G G C T G A G G A T C-3',S L A1-R:5'-G G A T T C T G G A A G G T T C T C A A T C C T T C C A T T T A T T T C C T C-3'㊂引物由吉林省库美生物科技有限公司合成㊂1.5S L A-1的扩增和测序利用O n e S t e p P C R试剂盒以及合成的引物对目的基因进行扩增㊂R T-P C R反应体系50μL:P r i-m e S c r i p t1S t e p E n z y m e M i x2μL,2ˑ1s t e p B u f f e r 25μL,上㊁下游引物(10p m o l㊃μL-1)各1.5μL,R N A 模板2μL(约300n g㊃μL-1),R N a s e F r e e H2O 18μL㊂P C R反应条件:50ħ反转录30m i n;94ħ预变性2m i n;94ħ变性30s,55ħ退火30s,72ħ延伸1m i n,35次循环;72ħ延伸8m i n㊂利用1%琼脂糖凝胶电泳对扩增产物进行鉴定,由吉林省库美生物科技有限公司直接测序㊂对于杂合子,利用胶回收试剂盒纯化目的片段,与p M D-18T载体在16ħ条件下连接2h,然后转化至大肠杆菌D H5α感受态细胞中,并利用含A m p抗性的L B平板进行筛选㊂鉴定阳性单克隆菌落后,送至吉林省库美生物科技有限公司测序㊂1.6序列比对及分析测序获得的原始序列数据通过C h r o m a s V e r-s i o n2.6.6进行人工编辑,校对电泳峰图与碱基对应关系㊂利用M E G A7.0软件对直接测序和克隆测序结果进行比对,根据不同猪个体中S L A-1基因序列差异确定等位基因序列㊂当同一个体克隆测序中至少3个单克隆的序列相同且该序列在不同个体的直接或克隆测序中多次出现,判定为一个等位基因,即一个等位基因的判定必须至少出现在2个不同的个体或来自2个独立的P C R反应㊂利用D n a S P5.10.01软件进行等位基因多态性分析,利用D N A S T A R L a s e r g e n e软件进行同源性分析,以人白细胞抗原(h u m a n l e u k o c y t e a n t i g e n,H L A)频率最高的H L A-A*02:01等位基因(G e n B a n k登录号:F N806801)为参照[21],利用M E G A7.0采用邻接法(N e i g h b o r-J o i n i n g,N-J)构建系统进化树㊂所有序列提交G e n B a n k获得登录号㊂将新等位基因提交国际动物遗传学学会(T h e I n t e r n a t i o n a l S o c i e-t y o f A n i m a l G e n e t i c s,I S A G)S L A命名委员会获得66037期刘弘毅等:实验用荣昌猪S L A -1等位基因鉴定及分子遗传特征分析官方命名㊂S L A 等位基因的命名采用H L A 命名系统,等位基因前两位数字用于指定具有相似D N A序列的等位基因组( 组 是基于系统发育分析和D N A 序列基序的识别);第3和第4位数字用于指定具有不同蛋白质序列的等位基因;第5和第6位数字用于指定因同义核苷酸替换而不同的等位基因;以冒号(:)作为区域分隔符㊂2 结 果2.1 R T -P C R 扩增与克隆R T -P C R 扩增获得1537b p 左右的目的条带(图1),清晰且单一,表明已成功扩增S L A -1基因㊂将27头猪的扩增产物全部进行直接测序,分析测序结果,发现4头猪测序结果为单峰(猪编号为3㊁9㊁13和27),且序列相同,表明是纯合子个体,对其中1头(3号猪)进行了克隆㊂其它23头猪均为套峰,表明为杂合子个体,将具有相同杂合套峰的个体进行分类后,分别对10个个体(猪编号为3㊁5㊁10㊁15㊁16㊁17㊁18㊁19㊁23和24)进行了克隆㊂所有克隆鉴定阳性单克隆菌落并测序(图2)㊂M.D L 2000D N A 分子标记;1.P C R 产物(猪编号2);2.P C R 产物(猪编号3);3.P C R 产物(猪编号5);4.P C R 产物(猪编号10);5.P C R 产物(猪编号14);6.P C R 产物(猪编号15);7.P C R 产物(猪编号17);8.P C R 产物(猪编号18)M.D L 2000D N A m a r k e r ;1.P C R p r o d u c t o f N o .2;2.P C R p r o d u c t o f N o .3;3.P C R p r o d u c t o f N o .5;4.P C Rpr o d u c t o f N o .10;5.P C R p r o d u c t o f N o .14;6.P C R pr o d u c t o f N o .15;7.P C R p r o d u c t o f N o .17;8.P C R pr o d u c t o f N o .18图1 S L A -1基因部分扩增结果F i g .1 P a r t i a l a m pl i f i c a t i o n r e s u l t s o f S L A -12.2 S L A -1等位基因序列分析分析27头荣昌猪S L A -1基因直接测序和克隆测序结果,根据等位基因的判定原则共鉴定出11个等位基因(表1),将其提交G e n B a n k 数据库和I P D -MH C 数据库,分别获得登录号和I S A G S L A 命名委员会的官方命名(表2)㊂与I P D -MH C 数据库中M.D L 2000D N A 分子标记;1~4.编号10的单克隆鉴定部分结果;5~8.编号14的单克隆鉴定结果M.D L 2000D N A m a r k e r ;1-4.P a r t i a l r e s u l t s o f n o .10m o n o c l o n a l i d e n t i f i c a t i o n ;5-8.P a r t i a l r e s u l t s o f n o .14m o n -o c l o n a l i d e n t i f i c a t i o n图2 部分阳性单克隆菌落P C R 扩增结果F i g.2 P a r t i a l r e s u l t s o f m o n o c l o n a l i d e n t i f i c a t i o n 已提交的89个S L A -1等位基因序列相比较,获得2个已知等位基因,S L A -1*11:03和S L A -1*15:03,分别存在于韩国地方猪种和中国融水小型猪㊁皖南花猪中㊂此外,发现9个新等位基因,命名为S L A -1*02:04㊁02:05㊁10:03㊁16:05㊁16:06㊁18:03㊁23:05㊁24:01和25:01㊂27头荣昌猪均至少包含1个S L A -1新等位基因,且10头荣昌猪中至少包含3个S L A -1等位基因,编号10的个体中含有4个不同的S L A -1等位基因,表明S L A -1是双拷贝基因㊂在该群体中,对被鉴定出在S L A -1基因座上具有两个以上等位基因的个体进行等位基因之间的两两组合分析,对在不同基因型个体中出现次数超过3次以上的组合确定为同一条染色体上的双拷贝等位基因,在编号分别为1㊁6㊁10㊁18㊁24㊁25的个体中不仅鉴定出S L A -1*23:05㊁S L A -1*11:03等位基因而且还有其他等位基因存在,因此在该群体中S L A -1*23:05与S L A -1*11:03,S L A -1*02:04与S L A -1*18:03之间存在连锁关系㊂连锁的等位基因S L A -1*23:05和S L A -1*11:03以及S L A -1*02:04和S L A -1*18:03分别用双拷贝等位基因A 和B表示,等位基因S L A -1*02:05㊁S L A -1*10:03㊁S L A -1*15:03㊁S L A -1*16:05㊁S L A -1*16:06㊁S L A -1*24:01㊁S L A -1*25:01分别用等位基因C ㊁D ㊁E ㊁F ㊁G ㊁H ㊁I 表示,该群体在S L A -1基因座上共获得11种基因型(表1)㊂群体的等位基因频率显示,频率最高的是等位基因H (S L A -1*24:01),频率为44.23%,其次是双拷贝等位基因A (S L A -1*23:05,S L A -1*11:03)和等位基因E (S L A -1*15:03),频率分别为19.23%㊁17.31%,H (S L A -1*24:01)是该荣昌猪群体中最流行的等位基因(图3)㊂7603畜 牧 兽 医 学 报54卷表1 荣昌猪S L A -1等位基因T a b l e 1 S L A -1a l l e l e o f R o n g c h a n g p i g荣昌猪编号N o .o f R o n g c h a n g性别S e xS L A -1等位基因A l l e l e s o f S L A -1S L A -1基因型1G e n o t y pe s of S L A -11123:05,11:03,24:01A H215:03,24:01E H324:01HH515:03,24:01E H623:05,11:03,15:03A E723:05,11:03,24:01A H823:05,11:03,24:01A H924:01HH 1023:05,11:03,02:04,18:03A B1115:03,24:01E H 1216:05,24:01F H 1324:01HH 1416:05,24:01F H 1515:03,16:06E G1616:05,24:01F H 1710:03,15:03,24:01-1823:05,11:03,02:05A C 1923:05,11:03,15:03A E2015:03,24:01E H 2116:05,24:01F H2310:03,24:01D H2423:05,11:03,25:01A I2523:05,11:03,16:05A F2615:03,24:01E H 2724:01HH 2010315:03,24:01E H2020323:05,11:03,24:01A H1.A~I 所表示的等位基因见表2;-.基因型无法判断1.T h e a l l e l e s r e p r e s e n t e d b y A t o I a r e m a r k e d i n t a b l e 2;-.G e n o t y pe c a n n o t b e d e t e r m i n e d 表2 荣昌猪S L A -1等位基因提交信息T a b l e 2 S u b m i s s i o n i n f o r m a t i o n f o r S L A -1a l l e l e i n R o n g c h a n g p i g 已鉴定等位基因I d e n t i f i e d a l l e l e等位基因A l l e l e G e n B a n k 登录号G e n B a n k a c c e s s i o n n u m b e rS L A -1*23:05,S L A -1*11:03A O N 859881,O P 249499S L A -1*02:04,S L A -1*18:03BO N 859887,O N 859888S L A -1*02:05C O N 859886S L A -1*10:03DO N 859883S L A -1*15:03E O P 249500S L A -1*16:05F O N 859884S L A -1*16:06G O N 859885S L A -1*24:01HO N 859880S L A -1*25:01IO N 85989086037期刘弘毅等:实验用荣昌猪S L A -1等位基因鉴定及分子遗传特征分析图3 S L A -1等位基因及频率分布F i g .3 T h e d i s t r i b u t i o n o f S L A -1a l l e l e s a n d f r e q u e n c y2.3 S L A -1等位基因多态性与二级结构分析11个S L A -1等位基因编码区(c o d i n g se -qu e n c e s ,C D S )D N A 多态性分析显示(图4),碱基变异(b a s e v a r i a t i o n ,S )主要集中在225~325b p 和500~575b p 两个区域,核苷酸多样度(n u c l e o t i d e d i v e r s i t y,P i )的变化趋势与碱基变异数的分布趋势基本保持一致,而且两者的峰值均处于第2和第3外显子区域内,表示该区域是S L A -1基因的高频变异位点区㊂在C D S 区内,整体的P i 为0.0449,单倍型多样度(h a p l o t y p e d i v e r s i t y,H d )为1,平均核苷酸差异数(a v e r a ge n u m b e r of n u c l e o t i d e d i f f e r e n c e s ,K )为48.782,G+C 含量为64.9%,在所编码的361个氨基酸中,产生了75个氨基酸变异位点(a m i n oa c i d v a r i ab l e s i t e s ,A V );在1~1086b p 的核苷酸位点中,涵盖了127个核苷酸多态位点,其中,包含简约信息位点85个,单一多态位点42个,此外,核苷酸多态位点包含18个同义单核苷酸多态性位点和109个非同义单核苷酸多态性位点㊂第2和第3外显子区域的多态性规律与整个C D S 区变异相似(表3)㊂图4 S L A -1等位基因碱基变异位置分布(A )和核苷酸多样度变化趋势(B )F i g .4 T h e d i s t r i b u t i o n o f b a s e v a r i a t i o n p o s i t i o n s (A )a n d n u c l e o t i d e d i v e r s i t y (B )o f S L A -1a l l e l e s e qu e n c e 由于S L A 提呈不同抗原,因此第2和第3外显子编码的α1和α2结构域构成的P B G 多态性较高,对荣昌猪S L A -1编码蛋白的α1和α2结构域的氨基酸序列变异性进行分析,结果显示,有14个氨基酸位点的W u -K a b a t 指数高于6.0,其中,第74个氨基酸指数最高,达到13.8(图5A )㊂第2外显子区域的氨基酸变异程度高于第3外显子,且变异程度最高的位点位于第2外显子区域㊂此外,由于S L A -1*04:01分子所编码的蛋白结构已经解析[22],共存在33个关键氨基酸组成P B G 的A~F 6个口袋,因此将获得的荣昌猪S L A -1的α1和α2结构域氨基酸序列与S L A -1*04:01进行比对分9603畜牧兽医学报54卷表3S L A-1等位基因序列多态性分析T a b l e3P o l y m o r p h i s m a n a l y s i s o f S L A-1a l l e l e s e q u e n c e s序列信息S e q u e n c e i n f o r m a t i o nS L A-1编码区S L A-1C D S r e g i o n第2和第3外显子E x o n2a n d e x o n3比对序列范围/b p A l i g n e d S e q.r e g i o n 1~108665~610核苷酸数N u m b e r o f n u c l e o t i d e 1086546核苷酸保守性位点数量N u m b e r o f n u c l e o t i d e i n v a r i a b l e s i t e s 959444核苷酸多态位点数量N u m b e r o f n u c l e o t i d e v a r i a b l e s i t e s 127102简约信息位点数量N u m b e r o f n u c l e o t i d e p a r s i m o n y i n f o r m a t i o n s i t e s 8565单一多态位点数量N u m b e r o f n u c l e o t i d e s i n g l e t o n v a r i a b l e s i t e s 4237同义单核苷酸多态性数量N u m b e r o f s y n o n y m o u s S N P(S i n g l e n u c l e o t i d e p o l y m o p h i s m)187非同义单核苷酸多态性数量N u m b e r o f n o s y n o n y m o u s S N P 10995氨基酸数量N u m b e r o f a m i n o a c i d s 361182氨基酸变异位点数量N u m b e r o f a m i n o a c i d s v a r i a b l e s i t e s 7561G+C含量G+C c o n t e n t 0.6490.653平均核苷酸差异数量A v e r a g e n u m b e r o f n u c l e o t i d e d i f f e r e n c e s 48.78239.145单倍型多样性H a p l o t y p e d i v e i t y 1.0001.000核苷酸多样性N u c l e o t i d e d i v e r s i t y0.04490.0717析,结果显示,182个氨基酸位点中,121个位点完全保守,而33个关键氨基酸位点中,11个氨基酸位点完全保守,分别为L e u5(L)㊁T y r7(Y)㊁M e t45(M)㊁T y r59(Y)㊁L e u81(L)㊁T y r84(Y)㊁T y r123(Y)㊁L y s146(K)㊁T y r159(Y)㊁L e u160(L)㊁T y r171(Y),其余22个位点均发生高频率突变(图5B)㊂比对H L A-A*02:01㊁H L A-A*11:01与荣昌猪S L A-1等位基因氨基酸序列,结果显示,在与β2-微球蛋白(β2-m i c r o g l o b u l i n,β2m)结合的19个氨基酸位点中,有10个氨基酸在人H L A-A与荣昌猪S L A-1等位基因中保持一致,分别是T y r7(Y)㊁P h e8(F)㊁V a l25(V)㊁T y r27(Y)㊁P h e33(F)㊁P h e36(F)㊁H i s93 (H)㊁L e u110(L)㊁G l y112(G)和G l n115(Q)㊂另外,比较人和荣昌猪C D8分子与MH C结合的7个关键氨基酸位点(223~229)[23],只有225(T/S)和228(T/M)两个位点存在差异,其它位点高度保守(表4)㊂二级结构分析发现,荣昌猪不同S L A-1等位基因显示出S L A-1基因的典型二级结构,α1和α2区主要为α-螺旋和β-折叠,α1区具有1个α-螺旋和4个β-折叠,α2区具有4个α-螺旋和4个β-折叠(图5B)㊂2.4S L A-1等位基因同源性分析荣昌猪不同S L A-1等位基因的核苷酸相似性为93.6%~99.4%,氨基酸相似性为86.5%~ 98.3%,其中,S L A-1*02:04和S L A-1*02:05同源性最高,核苷酸和氨基酸相似性分别为99.4%和98.3%,二者存在7个核苷酸变异和6个氨基酸变异㊂荣昌猪与I P D-MH C数据库中已提交的69个具有C D S全长序列的S L A-1等位基因相似性分析显示,核苷酸序列和氨基酸序列分别为92.6%~07037期刘弘毅等:实验用荣昌猪S L A -1等位基因鉴定及分子遗传特征分析▼.人白细胞抗原(H L A )与β2-微球蛋白(β2m )结合位点,*.S L A -1*04:01等位基因抗原肽结合槽关键位点▼.A m i n o a c i d p o s i t i o n b i n d i n g h u m a n l e u k o c y t e a n t i g e n (H L A )a n d β2-m i c r o g l o b u l i n (β2m ),*.K e y a m i n o a c i d p o s i t i o n o f P B G l o c a t e d i n S L A -1*04:01a l l e l e 图5 荣昌猪S L A -1等位基因第2和第3外显子区域氨基酸变异性(A )和氨基酸序列比对(B )F i g .5 T h e a m i n o a c i d v a r i a b i l i t y (A )a n d c o m p a r i s o n (B )o f e x o n 2a n d e x o n 3o f S L A -1a l l e l e s i n R o n g c h a n g p i g99.9%和85.9%~99.4%㊂荣昌猪S L A -1等位基因和人群中流行的H L A -A *02:01和H L A -A *11:01等位基因的核苷酸相似性为83.3%~86.8%,氨基酸相似性为70.3%~76.9%,其中,与H L A -A *02:01相似性最高的等位基因是S L A -1*10:03(核苷酸85.0%,氨基酸74.4%),与H L A -A *11:01相似性较高的等位基因是S L A -1*18:03(核苷酸86.8%,氨基酸76.9%)(表5)㊂2.5 S L A -1等位基因系统进化树分析从I P D -MH C 数据库中选取与荣昌猪S L A -1等位基因核苷酸相似性较高(93.3%~99.9%)的31个具有C D S 全长的S L A -1等位基因,与荣昌猪获得的11个S L A -1等位基因序列比对,以人群中H L A -A 频率最高的H L A -A *02:01和H L A -A*11:01等位基因为外群对照,利用邻接法构建系统进化树(图6)㊂结果显示,荣昌猪中9个新等位基因分布在不同聚类分支中,根据系统进化树,I S A GS L A 命名委员会对其进行了命名㊂S L A -1*02:04和S L A -1*02:05聚在02等位基因组分支中,S L A -1*02:05与S L A -1*02:02具有3个核苷酸差异,S L A -1*02:04与S L A -1*02:01具有4个核苷酸差异,与多种猪品种和猪细胞系亲缘关系较近,如:1703畜牧兽医学报54卷表4人和荣昌猪C D8分子与M H C结合的关键氨基酸氨基酸位点比较T a b l e4C o m p a r i s o n o f k e y a m i n o a c i d p o s i t i o n o f h u m a n a n d R o n g c h a n g p i g C D8m o l e c u l e s c o m b i n e w i t h M H C等位基因A l l e l e氨基酸位置A m i n o a c i d p o s i t i o n 223224225226227228229H L A-A*02:01A s p(D)G l n(Q)T h r(T)G l n(Q)A s p(D)T h r(T)G l u(E) H L A-A*11:01ӘӘӘӘӘӘӘS L A-1*02:04ӘӘS e r(S)ӘӘM e t(M)ӘS L A-1*02:05ӘӘS e r(S)ӘӘM e t(M)ӘS L A-1*10:03ӘӘS e r(S)ӘӘM e t(M)ӘS L A-1*11:03ӘӘS e r(S)ӘӘM e t(M)ӘS L A-1*15:03ӘӘS e r(S)ӘӘM e t(M)ӘS L A-1*16:05ӘӘS e r(S)ӘӘM e t(M)ӘS L A-1*16:06ӘӘS e r(S)ӘӘM e t(M)ӘS L A-1*18:03ӘӘS e r(S)ӘӘM e t(M)ӘS L A-1*23:05ӘӘS e r(S)ӘӘM e t(M)ӘS L A-1*24:01ӘӘS e r(S)ӘӘM e t(M)ӘS L A-1*25:01ӘӘS e r(S)ӘӘM e t(M)ӘӘ.与H L A-A*02ʒ01相同的氨基酸Ә.S a m e a m i n o a c i d w i t h H L A-A*02:01表5荣昌猪S L A-1和I P D-M H C数据库中提交的S L A-1以及人H L A-A流行等位基因的相似性比较T a b l e5C o m p a r i s o n o f n u c l e o t i d e a n d a m i n o a c i d h o m o l o g y a m o n g S L A-1a l l e l e s o f R o n g c h a n g p i g,S L A-1a l l e l e s s u b m i t t e d i nI P D-M H C d a t a b a s e a n d f r e q u e n t h u m a n H L A-A a l l e l e s%同源性H o m o l o g y荣昌猪S L A-1等位基因S L A-1a l l e l e s o fR o n g c h a n g p i gI P D-MH C数据库S L A-1等位基因S L A-1a l l e l e s o fI P D-MH C d a t a b a s eH L A-A*02:01等位基因H L A-A*02:01a l l e l eH L A-A*11:01等位基因H L A-A*11:01a l l e l e核苷酸相似性N u c l e o t i d e h o m o l o g y 93.6~99.492.6~99.983.3~85.085.1~86.8氨基酸相似性A m i n o a c i d h o m o l o g y86.5~98.385.9~99.770.3~74.472.8~76.9辛克莱小型猪㊁N I H小型猪和小型猪肾细胞系等㊂S L A-1*10:03等位基因与汉福德㊁大花白以及融水小型猪中获得的S L A-1*10:01等位基因聚于同一分支,与S L A-1*10:01具有5个核苷酸差异㊂S L A-1*16:05和S L A-1*16:06遗传距离较近,与S L A-1*16:03聚为一支,属于16等位基因组,特别是S L A-1*16:05与S L A-1*16:03仅有一个核苷酸差异,两个等位基因均与亚洲野猪的亲缘关系较高㊂S L A-1*18:03与S L A-1*18:01聚于同一分支㊂S L A-1*23:05独立聚为一支,表明有特定的肽结合基序,而且与S L A-1*11:10和S L A-1*23:01聚为一大类分支,表明与巴马小型猪亲缘关系较近㊂S L A-1*24:01与亚洲野猪㊁巴马小型猪中获得的S L A-1*19:01聚于一大支,表明荣昌猪和其亲缘关系较近㊂S L A-1*25:01独立分为一支,表明也具有独特的肽结合基序㊂此外,已知的S L A-1*11:03和S L A-1*15:03等位基因在韩国地方猪㊁中国融水小型猪和皖南花猪也存在,表明这些品种猪可能有相同血缘关系的祖先㊂3讨论实验猪在畜牧兽医生产实践中发挥重要作用,被广泛用于研究病原致病机制㊁T细胞表位识别和疫苗评估等,在这些研究中S L A对抗原的识别具有严格的MH C限制性,从而引起个体差异[24-27]㊂因此鉴定S L A基因可减少实验动物遗传背景差异对各项研究结果的影响㊂经典的S L A I类基因S L A-1编码的I类分子主要参与内源性抗原递呈,识别27037期刘弘毅等:实验用荣昌猪S L A -1等位基因鉴定及分子遗传特征分析һ.荣昌猪S L A -1等位基因һ.S L A -1a l l e l e s o f R o n g c h a n g p i g 图6 荣昌猪S L A -1等位基因系统发育树F i g .6 P h y l o g e n e t i c t r e e o f S L A -1a l l e l e f r o m R o n g c h a n g p i g3703畜牧兽医学报54卷抗原并诱导细胞免疫应答㊂S L A-1基因具有高度多态性,目前I P D-MH C数据库共收录了89个S L A-1等位基因(R e l e a s e3.9.0.107/2022b u i l d209),存在于不同的猪品种或猪细胞系㊂荣昌猪是我国优良的地方猪品种,已作为实验动物在动物医学模型㊁药物评价模型和异种器官供体等研究中广泛应用,但目前尚未对荣昌猪的S L A-1基因进行系统鉴定,本研究在荣昌猪S L A-1基因座上共获得11个等位基因,其中,9个为新等位基因,2个为已知等位基因,在现有群体数量有限的情况下S L A-1基因座表现出较高的多态性㊂荣昌猪S L A-1基因也发现了双拷贝现象㊂在之前的研究中,有学者发现S L A-1基因座双拷贝现象在韩国本地猪㊁S N U小型猪㊁N I H小型猪㊁巴克夏猪㊁约克夏猪㊁杜洛克猪㊁长白猪㊁梅山猪和巴马小型猪等众多猪品种中均存在,且部分双拷贝现象在某些S L A单倍型中持续存在[28-31]㊂一般认为,基因多样性或杂合度的增加有利于机体抵御不良环境,但无论是不同S L A基因座的等位基因杂合还是S L A-1基因座双拷贝杂合,在病原体和S L A等位基因之间的关系都是较为复杂的㊂首先,在同一个S L A基因座存在多个等位基因可能会增加其对不同抗原的结合能力,从而提高生存概率;其次,如果在群体中不存在对特定病原敏感的特定等位基因,即便杂合度很高,该种群对于病原的抵抗力依然较弱,而存在特定等位基因的纯合子群体却能够获得高的抵抗力,这可以从杂合子优势假说以及稀有等位基因优势假说得到解释[32-33]㊂S L A-1基因的第2和第3外显子所编码的抗原肽结合槽相较于其它区域具有非常高的多态性,这种现象在不同猪品种中都存在[34-36]㊂本研究对荣昌猪S L A-1等位基因的D N A多态性进行了分析,从核苷酸多态性位点数量占比和非同义核苷酸多态性位点数量的比例发现,第2和第3外显子区域都表现出绝对的优势㊂氨基酸变异位点数量以及氨基酸变异系数和位置与核苷酸多态性具有相似性,同样证明S L A-1基因的高度多态区域集中于第2和第3外显子㊂S L A I类分子主要通过递呈病原的抗原肽给C D8+T淋巴细胞识别[37],继而诱导细胞杀伤效应清除感染性细胞㊂抗原肽是通过结合S L A I类分子P B G中的6个口袋(A~F),然后与β2m形成复合物递呈在细胞表面供T细胞识别[38-39]㊂因此不同等位基因和递呈抗原肽的差异会对P B G的立体结构造成影响,利用已解析的S L A-1*04:01的三维结构,对荣昌猪S L A-1等位基因6个口袋的关键氨基酸进行比对,发现有较高的差异性,提示获得的等位基因可能展现出与S L A-1*04:01不同的三维结构㊂猪已被选定是最佳的异种移植供体,但猪-人异种器官/细胞移植主要障碍是人体对植入外源器官的免疫反应,其中S L A是与同种和异种免疫识别密切相关的一个重要分子标记基因,能被各种人类免疫细胞亚群直接识别[40]㊂因此异种移植成功与否的前提在于猪S L A与人H L A之间的适配性所导致的急性或慢性免疫排斥反应能否得到解决㊂比较荣昌猪S L A-1与β2m结合以及人和荣昌猪C D8分子与MH C结合的关键氨基酸位点,发现氨基酸位点保守性较高㊂将获得的S L A-1等位基因与人群体中频率较高的等位基因进行同源性分析,显示S L A-1*10:03和S L A-1*18:03相似性最高,预示着携带这两个等位基因的猪可能和人之间存在较高的适配性㊂目前,I P D-MH C数据库已收录了世界各类猪种以及猪细胞系的S L A-1等位基因,选取与荣昌猪相似性较高的S L A-1等位基因建立了系统进化树,发现荣昌猪种群中获得的S L A-1*15:03和S L A-1*11:03也分别存在于中国融水小型猪㊁皖南花猪以及韩国本地猪中,这些猪种起初都源自亚洲㊂获得的新S L A-1等位基因少部分与各类猪源细胞系亲缘性较高,大多数均与亚洲猪种亲缘性较高,因此可推测荣昌猪可能和这些猪种存在血缘相同的祖先㊂本研究获得的荣昌猪S L A-1等位基因遗传信息仅针对于饲养于屏障设施中这个小型封闭群,并不能代表全国整个荣昌猪品种的群体遗传信息,但是对标准化实验荣昌猪的培育和推广应用必不可少㊂此外在荣昌猪上获得的S L A-1基因也可用于深入研究病原的抗原递呈机制,筛选猪重要病原的S L A限制性T细胞表位,对开发安全有效的表位肽疫苗意义重大㊂4结论本研究通过对荣昌猪S L A-1基因C D S区域进行P C R扩增测序,成功获得了11个S L A-1等位基因,其中,9个为新发现等位基因,S L A-1*24:01是47037期刘弘毅等:实验用荣昌猪S L A-1等位基因鉴定及分子遗传特征分析该荣昌猪群体中最流行的等位基因㊂S L A-1基因多态性主要集中在第2和第3外显子区域,P B G中的33个关键氨基酸位点有11个位点完全保守;与β2m结合的19个关键氨基酸位点有10个位点在人与荣昌猪之间保持一致,C D8分子与MH C结合的关键氨基酸位点高度保守,只有225(T h r/S e r)和228(T h r/M e t)位点不同㊂同源性以及系统进化树表明,S L A-1*10:03和S L A-1*18:03分别与人H L A-A*02:01和H L A-A*11:01等位基因的同源性最高,荣昌猪与亚洲野猪㊁巴马小型猪和融水小型猪等亚洲猪种具有较近的亲缘关系㊂本研究结果为荣昌猪S L A遗传背景的研究以及抗原递呈过程中S L A与不同抗原肽的相互作用关系奠定基础㊂参考文献(R e f e r e n c e s):[1]马丽颖,岳秉飞.主要组织相容性复合体(MH C)研究进展[J].中国比较医学杂志,2005,15(3):182-185.MA L Y,Y U E B F.A d v a n c e s i n r e s e a r c h o n m a j o rh i s t o c o m p a t i b i l i t y c o m p l e x[J].C h i n e s e J o u r n a l o fC o m p a r a t i v e M e d i c i n e,2005,15(3):182-185.(i nC h i n e s e)[2]刘子展.贵州小型猪S L A经典Ⅰ类和Ⅱ类基因克隆和生物信息学分析及P C R-S S P分型方法的建立[D].合肥:安徽农业大学,2012.L I U Z Z.C l o n i n g a n d b i o i n f o r m a t i c s a n a l y s i s o fs w i n e l e u k o c y t e a n t i g e n s c l a s s I a n d c l a s s I I g e n e s i nG u i z h o u m i n i p i g s a n d e s t a b l i s h m e n t o f P C R-S S Pt y p i n g m e t h o d[D].H e f e i:A n h u i A g r i c u l t u r a lU n i v e r s i t y,2012.(i n C h i n e s e)[3] Y AMA G U C H I T,D I J K S T R A J M.M a j o rh i s t o c o m p a t i b i l i t y c o m p l e x(MH C)g e n e s a n d d i s e a s er e s i s t a n c e i n f i s h[J].C e l l s,2019,8(4):378. [4] C A R R I N G T O N M,B O N T R O P R E.E f f e c t s o fMH C c l a s s I o n H I V/S I V d i s e a s e i n p r i m a t e s[J].A I D S,2002,16:S105-S114.[5] R O C K K L,R E I T S E,N E E F J E S J.P r e s e n tY o u r s e l f!B y MH C c l a s s I a n d MH C c l a s s I Im o l e c u l e s[J].T r e n d s I mm u n o l,2016,37(11):724-737.[6] S E E T H A R A M A,T I R I V E E D H I V,M O H A N A K U M A RT.A l l o i mm u n i t y a n d a u t o i mm u n i t y i n c h r o n i cr e j e c t i o n[J].C u r r O p i n O r g a n T r a n s p l a n t,2010,15(4):531-536.[7] A F Z A L I B,L OM B A R D I G,L E C H L E R R I.P a t h w a y s o f m a j o r h i s t o c o m p a t i b i l i t y c o m p l e xa l l o r e c o g n i t i o n[J].C u r r O p i n O r g a n T r a n s p l a n t,2008,13(4):438-444.[8] W E I S S R A.X e n o t r a n s p l a n t a t i o n[J].B M J,1998,317(7163):931-934.[9] C O O P E R D K C,G A S T O N R,E C K HO F F D,e t a l.X e n o t r a n s p l a n t a t i o n-t h e c u r r e n t s t a t u s a n d p r o s p e c t s[J].B r M e d B u l l,2018,125(1):5-14.[10] C E L I S-G I R A L D O C T,B OHÓR Q U E Z M D,C AMA R G O M,e t a l.A c o m p a r a t i v e a n a l y s i s o fS L A-D R B1g e n e t i c d i v e r s i t y i n C o l o m b i a n(c r e o l e sa n d c o mm e r c i a l l i n e)a n d w o r l d w i d e s w i n ep o p u l a t i o n s[J].S c i R e p,2021,11(1):4340. [11]权金强,江新杰,李昌文,等.实验用S P F大白猪和长白猪S L A-1基因特征分析[J].农业生物技术学报,2017,25(5):770-780.Q U A N J Q,J I A N G X J,L I C W,e t a l.C h a r a c t e r i z a t i o n a n a l y s i s o f S L A-1g e n e f r o m S P Fy o r k s h i r e(S u s s c r o f a)a n d l a n d r a c e[J].J o u r n a l o fA g r i c u l t u r a lB i o t e c h n o l o g y,2017,25(5):770-780.(i n C h i n e s e)[12] HAMM E R S E,HO C S,A N D O A,e t a l.I m p o r t a n c e o f t h e m a j o r h i s t o c o m p a t i b i l i t y c o m p l e x(S w i n e l e u k o c y t e a n t i g e n)i n s w i n e h e a l t h a n db i o m e d ic a l r e s e a r c h[J].A n n u R e v A n i m B i o s c i,2020,8:171-198.[13] G A O C X,H E X W,Q U A N J Q,e t a l.S p e c i f i c i t yc h a r a c t e r i z a t i o n o f S L A c l a s s I m o l e c u l e s b i nd i n g t os w i n e-o r i g i n v i r a l c y t o t o x i c T l y m p h o c y t e e p i t o p ep e p t i d e s i n v i t r o[J].F r o n t M i c r o b i o l,2017,8:2524.[14] L U N N E Y J K.A d v a n c e s i n s w i n e b i o m e d i c a l m o d e lg e n o m i c s[J].I n t J B i o l S c i,2007,3(3):179-184.[15] S W I N D L E M M,MA K I N A,H E R R O N A J,e t a l.S w i n e a s m o d e l s i n b i o m e d i c a l r e s e a r c h a n dt o x i c o l o g y t e s t i n g[J].V e t P a t h o l,2012,49(2):344-356.[16] G E I S L E R K,KÜN Z E L J,G R U N D T N E R P,e t a l.T h e p e r f u s e d s w i n e u t e r u s m o d e l:l o n g-t e r m p e r f u s i o n[J].R e p r o d B i o l E n d o c r i n o l,2012,10:110.[17] R AM I R E Z-M E D I N A E,V U O N O E,R A I A,e t a l.D e l e t i o n o f E184L,a p u t a t i v e D I V A t a r g e t f r o m t h ep a n d e m i c s t r a i n o f a f r i c a n s w i n e f e v e r v i r u s,p r o d u c e sa r e d u c t i o n i n v i r u l e n c e a n d p r o t e c t i o n a g a i n s t v i r u l e n tc h a l l e n g e[J].J V i r o l,2022,96(1):e0141921.[18] C H E N W,Y I H J,Z HA N G L,e t a l.E s t a b l i s h i n gt h e s t a n d a r d m e t h o d o f c o c h l e a r i m p l a n t i nR o n g c h a n g p i g[J].A c t a O t o-L a r y n g o l,2017,1375703。
Fitness Madness 19ASSEMBLY INSTRUCTIONS说明书
5740AH - 40 Ton Air/Hydraulic Shop Press 5750AH - 50 Ton Air/Hydraulic Shop PressCONTENTS:Page 1Specifications2Safety Information and Warranty Information 3Parts List4-6Assembly Instructions789Pump and Ram Assembly Instructions Procedure for Bleeding Air Pump Instructions10-11Winch Kit Parts List and Winch Installation Instructions 12Replacement Parts List© Copyright 2020, Sunex Tools ®SAFETY INFORMATIONThis symbol alerts you to the possibility of serious injury or death if instructions are not followed.This symbol alerts you to the possibility of damage to or destruction of equipment if instructions are not followed.Failure to heed these warnings may result in lossof load, damage to the press and/or failure resulting in property damage, personal or fatal injury. Thisoperating manual contains important details concern-ing the safe operation of this tool. The user must read and understand these details before any use of the tool. This manual must be retained for future reference.• Read, study, understand and follow all instructions before operating this press.• Always wear safety goggles, (users and bystanders).•Parts being pressed may splinter, shatter, or be ejected from the press at a dangerous rate of speed. Because there are a variety of press applications, it is the responsibility of the press owner to provide adequate guards, eye protection and protective clothing to the press operator.• Visual inspection of the press should be made before use, checking for signs of cracked welds, bent bed pins, loose or missing bolts, leaks, or any other structural damage. Corrections must be made before using the press.•Do not go near leaks. High pressure hydraulic uid can puncture skin and cause serious injury,gangrene, or death. If injured, seek emergency medical help as immediate surgery is required to remove the uid.• Prior to use make sure the press is securely anchored to a concrete oor.•Keep hands, arms, feet, and legs out of work area. Accidental slippage can result in personal injury.• Always use an accurate force gauge to measure pressing force.• Do not exceed the rated capacity or tamper with the pressure/force settings. When attachments and adapters are used the rated capacity of the system shall be no greater than the rated capacity of the lowest rated component or combination of components that make up the system. • Avoid off-center loads. Offset loads can damage ram and may cause load to eject at a dangerous rate of speed. Do not use any spacer or extender between the press ram plunger and the item being pressed. If there is not enough ram stroke, adjust the height of the movable bolster.•Remove all loads from movable bolster before attempting to adjust bolster height. Beware of possible falling bolster.•Press only on loads supported by movable bolster and press plates included. Do not support loads on oor or press frame legs.• When using any accessories such as press plates or arbor plates, be certain they are centered on the movable bolster and in full contact with both sides of the bolster.• Before applying load, be certain all movable bolster supporting pins are fully engaged. Verify lift cables (if equipped) are slack before pressing on the bolster.• Always use a bearing shield when pressing bearings.•Use caution when positioning work to be pressed to ensure the item to be pressed cannot be ejected at a dangerous rate of speed.• Release hydraulic pressure before loosening any ttings.• Maintain proper hydraulic uid levels.• Do not make any alterations or modi cations to the press.•This product may contain one or more chemicals known to the State of California to cause cancer and birth defects or other reproductive harm. Wash hands thoroughly after handling.•Failure to heed and understand these markings may result in serious or fatal personal injury and/or property damage.OWNER/USER RESPONSIBILITYThe owner and/or user must have an understanding of the manufacturer'soperating instructions and warnings before using this press. Personnel involvedin the use and operation of equipment shall be careful, competent, trained,and quali ed in the safe operation of the equipment and its proper use whenservicing motor vehicles and their components. Warning information shouldbe emphasized and understood.If the operator is not uent in English, the manufacturer's instructions andwarnings shall be read to and discussed with the operator in the operator'snative language by the purchaser/owner, making sure that the operatorcomprehends its contents.Owner and/or user must study and maintain for future reference the manufactur-er’s instructions. Owner and/or user is responsible for keeping all warning labels and instruction manuals legible and intact. Replacement labels and literature are available from the manufacturers.INSPECTIONVisual inspection of the shop press should be made before each use of the press, checking for damaged, loose or missing parts. Each press must be inspected by a manufacturer’s repair facility immediately, if subjected to an abnormal load or shock. Any press which appears to be damaged in any way, is found to be badly worn, or operates abnormally must be removed fromservice until necessary repairs are made by a manufacturers's authorized repair facility. It is recommended that an annual inspection of the press be made by a manufacturer’s authorized repair facility and that any defective parts, decals or warning labels be replaced with manufacturer’s speci ed parts. A list of authorized repair facilities is available from the manufacturer.SAFETY INSTRUCTIONS•CHECK YOUR LOCAL, STATE AND FEDERAL REGULATIONSREGARDING THE SAFE USE OF THIS EQUIPMENT.•Your safety is top priority. Please handle equipment with care.•Fully retract unit and remove all items from the press bed frame.•Support the press bed, and remove the pins.•Raise or lower bed to desired height and reinstall press pins. Be certainpins are fully engaged in the parallel anges of the upright columns.•Position press on a at, level, hard surface, preferably concrete.Make sure all nuts and bolts are tight.•Clear the area of bystanders, especially small children, before using.•Set the press bed to the required height. The press is most effectivewhen the work piece is located 1 inch below the ram’s retracted position.The compression stroke can include the entire 5 inch working range.•The press is designed to exert a force on anything which is positionedbeneath its ram. The work piece can pop out from under the ram at a high rate of speed and injure someone.•Pressing Bearings: It is essential that you use the bearing shield whenpressing bearings on or off.LIMITED WARRANTY:SUNEX INTERNATIONAL, INC. WARRANTS TO ITS CUSTOMERS THAT THE COMPANY’S SUNEX TOOLS ® BRANDED PRODUCTS ARE FREE FROM DEFECTS IN WORKMANSHIP AND MATERIALS.Sunex International, Inc. will repair or replace its Sunex T ools ® branded products which fail to give satisfactory service due to defective workmanship or materials, based upon the terms and conditions of the following described warranty plans attributed to that speci c product. This product carries a ONE-YEAR warranty. During this warranty period, Sunex T ools ® will repair or replace at our option any part or unit which proves to be defective in material or workmanship. Other important warranty information....This warranty does not cover damage to equipment or tools arising fromalteration, abuse, misuse, damage and does not cover any repairs or replace-ment made by anyone other than Sunex Tools ® or its authorized warranty service centers. The foregoing obligation is Sunex Tools ®’ sole liability under this or any implied warranty and under no circumstances shall we be liable for any incidental or consequential damages. Note: Some states do not allow the exclusion or limitation of incidental or consequential damages, so the abovelimitation or exclusion may not apply to you. Return equipment or parts to Sunex Tools ®, transportation prepaid. Be certain to include your name and address, evidence of the purchase date, and description of the suspected defect.If you have any questions about warranty service, please write to Sunex Tools ®.This warranty gives you speci c legal rights and you may also have other rightswhich vary from state to state. Repair kits and replacement parts are available for many of Sunex Tools ® products regardless of whether or not the product is still covered by a warranty plan.SHIPPING ADDRESS: MAILING ADDRESS:Sunex Tools Sunex Tools 315 Hawkins Rd. P .O. Box 4215Travelers Rest, South Carolina 29690 Greenville, South Carolina 29608THIS OPERATING MANUAL CONTAINS IMPORTANT SAFETY INFORMATION. READ CAREFULLY AND UNDERSTAND ALL INFORMATION BEFORE OPERATING THIS TOOL. SAVE THIS MANUAL FOR FUTURE USE.WARNING: This product can expose you to chemicals including nickel, which is known to the State of California to cause cancer and birth defects or other reproductive harm.For more information go to .Press Frame - 1Press Bed - 1Press Feet - 2Z Bar - 11/2"-13 x 1-1/2" Hex Head Bolts - 101/2" Nuts - 10Air MotorPump and Ram - 1Pump Extension Handle - 11" x 3" x 14"Press Plates - 2Accessory only -Not needed for assemblyPushing Adapter - 1Accessory only -Not needed for assemblyBearing Shield - 1Accessory only -Not needed for assemblyESTIMATED ASSEMBLY TIME: 30 MINUTESACCESSORIESASSEMBL CONTINUEDPUMP AND RAM ASSEMBL Y INSTRUCTIONSPROCEDURE FOR BLEEDING AIRPUMP INSTRUCTIONSWINCH INSTALLATION INSTRUCTIONS CONTINUED。
7LS2A型采煤机技术协议详解
One (1) Hydraulic Pick Sleeve Extractor 1 个液压截齿套拆卸装置
2 x light; 4 x cameras 2 个照明灯和 4 个矿用照相机
One (1) Lubrication System 1 套润滑系统
One (1) Electrical Controller Section containing the Faceboss Electronic Controls including the JNA CCU unit and Display Computer. In both Chinese and English 1 套电控系统，包含 Faceboss 装置和显示器，Faceboss 电控系统包括 JNA 和 CCU 单元。中英 文显示。
Two (2) Butt Plates (right &left hand) 2 个摇臂座(左侧和右侧)
Two (2) 1.8m Kennametal Cutting Drums (right & left hand) including bit holders and bits 2 个 1.8 米的肯纳金属生产的切割滚筒 (左切割滚筒和右切割滚筒), 包括齿座和截齿。
Headgate System with Tele-Remote Equipment includes: 带远程监控的顺槽通讯控制系统,包括： (1) One (1) Data Transmission and display system-JOS 一套数据传输和显示系统-标准顺槽系统 The JNA Outbye System (JOS) is a computer-based data acquisition and control system. Operators can use the JOS to monitor data from the Shearer, AFC, Joy PRS and Switchgear. 久益顺槽系统（JOS）是以计算机为基础的数据获取和控制系统。操作者通过 JOS 可以监控运输 机，采煤机，支架及开关柜的数据。
Leviton HDX Unity Base8 24 Breakout Cassettes 商品说明
Page 1 of 2APPLICATIONHDX Unity Base8/24 Breakout Cassettes are designed for connection to electronics with LC-style ports and aggregating SFP ports to QSFP , QSFP28, QSFP-DD, and OSFP 28 ports. The cassettes manage polarity to maintain, transmit, and receive connections between parallel optics and SFP connectivity. The cassette easily snaps in and out ofrack-mount panels, facilitating rapid installation, adds, and changes in the network. Breakout cassettes are ideal for applications including 40G to 10G, 120G to 10G, 120G to 40G, and can support data rates beyond 100G.SPECIFICATIONAll pre-terminated components, including trunks, cassettes, harnesses, brackets, patch panels, and enclosures, shall be provided and warranted by a single manufacturer. Multimode cassettes shall meet an optical insertion loss not to exceed 0.35 dB for Base8 MTP-to-LC cassettes, 0.50 dB for Base24 MTP-to-LC cassettes, or 0.75 for Base24MTP-to-MTP cassettes. Single-mode cassettes shall meet an optical insertion loss not to exceed 0.50 dB for Base8 and Base24 MTP-to-LC cassettes, or 0.75 dB for Base24MTP-to-MTP cassette. LC adapters shall use ceramic ferrules and shall provide an option for IP5x-rated internal shutters, compliant with IEC 60529, that open and close automatically with the insertion and removal of an LC connector. MTPconnectors shall mate to key up/key down adapter on rear of cassette. Cassettes shall be pre-terminated and pre-tested with test documentation provided upon request. Cassettes available in breakout polarities for use with Method B Trunk or array cords and shall allow for multiple cassettes to be connected in a channel.STANDARDS & REGULATIONS• Supports data rates identified in multiple IEEE 802.3xx standards as applicable by connector type and cable grade • ANSI/TIA-942 data center design guidelines• ANSI/TIA-568.3-D for performance requirements • ANSI/TIA-604-5 (MTP), -10B (LC) for connector intermateability• IEC 60529 IP5x dust protection (LC)OPT-X™ HDX Unity Ultra Low Loss Base8/24 Breakout MTP ® CassettesOPT-X HDX Unity Base8/24 Breakout MTP CassettesFEATURES• Ultra Low-Loss Base8 and Base24 MTP Connectors for maximum network design flexibility• Select from a variety of fiber types including single-mode (OS2), laser-optimized multimode (OM3 and OM4), SWDM multimode (OM5)• Cassettes use bend-insensitive fiber• Patented internal shutters on LC adapters eliminate the need for dust plugs to reduce waste, accelerate deployment, and ensure safer installation.• Compatible with reverse polarity LC Uniboot patch cords • HDX cassettes fit in all UHDX and HDX enclosure or panel configurations• Same cassette used on each end of fiber channelDESIGN CONSIDERATIONS• Network design flexibility between Core, Aggregation, and Network switches• To port replicate or breakout switch ports such as:• Arista 7500 Series switches • Brocade VDX switches• Extreme Networks Black Diamond Series switches • Cisco Nexus 7000 Series switches• 4:1 breakout cassette uses Base8 MTP connectors (male)• 3:1 or 12:1 breakout cassettes uses Base24 MTP connectors (male)• Cassettes to be used with Method B trunksMECHANICAL SPECIFICATIONSDimensions: See page two Materials: Gray polycarbonateTemperature (Installation): -10 °C to +60 °C Temperature (Operating): -10 °C to +60 °CWARRANTY INFORMATIONFor Leviton product warranties, go to /ns/warrantyPERFORMANCE SPECIFICATIONOPT-X HDX Unity Base8/24 Breakout MTP Cassettes24F, 4:1 8F MTP to LC24F, 3:1 MTP to MTPCOUNTRY OF ORIGINPlease contact customer serviceUSANetwork Solutions Headquarters +1 (800) 722 2082 *******************Leviton Berk-Tek Cable : +1 (800) 237 5835 ************************Asia Pacific+852 3620 2602********************Canada+1 (800) 461 2002**********************Europe+44 (0) 1592 772124 **********************Latin AmericaMX: +52 (55) 2128 6286 LATAM: +52 (55) 2333 5963 *********************Middle East & Africa +971 (4) 247 9800 *******************NETWORK SOLUTIONS PRODUCTS ARE AVAILABLE WORLDWIDE IN OVER 100 COUNTRIES. VISIT US ONLINE AT /NS TO LEARN MORE.Page 2 of 2For further support information, visit /ns/supportOPT-X HDX Unity Base8/24 Breakout MTP CassettesOPT-X™ HDX Unity Base8/24 Breakout MTP ® CassettesOPT-X HDX UNITY BASE8/24 BREAKOUT MTP CASSETTESDescriptionPart No.HDX OM4, 8-fiber, MTP ® (male) to LC shuttered (aqua), 4:1 breakout 48LSM1-41C HDX OM4, 8-fiber, MTP (male) to LC shuttered (heather violet), 4:1 breakout 48GSM1-41C HDX OM5, 8-fiber, MTP (male) to LC shuttered (lime green), 4:1 breakout M58GSM1-41C HDX OS2, 8-fiber, MTP (male) to LC shuttered (blue), 4:1 breakoutU8LSM1-41C HDX2 OM4, 3x8-fiber, MTP (male) to 24-fiber LC shuttered (aqua), 4:1 breakout 42LSM1-41C HDX2 OM4, 3x8-fiber, MTP (male) to 24-fiber LC shuttered (heather violet), 4:1 breakout 42GSM1-41C HDX OM5, 3x8-fiber, MTP (male) to LC shuttered (lime green), 4:1 breakout M52GSM1-41C HDX2 OS2, 3x8-fiber, MTP (male) to 24-fiber LC shuttered (blue), 4:1 breakout U2LSM1-41C HDX2 OM3, 24-fiber, MTP (male) to 24-fiber shuttered LC (aqua) 12:1 breakout 32LSM2-21C HDX2 OM4, 24-fiber, MTP (male) to 24-fiber shuttered LC (heather violet) 12:1 breakout 42GSM2-21C HDX2 OM5, 24-fiber MTP (male) to 24-fiber LC shuttered (lime green), 12:1 breakout M52GSM2-21C HDX2 OM4, 24-fiber, MTP (male) to 3x8-fiber MTP (male), 3:1 breakout 42NM2-S5C HDX2 OS2, 24-fiber, MTP (male) to 3x8-fiber MTP (male), 3:1 breakoutU2NM2-S5CFor other standard cassette configurations, see Online Configurator at /configurator3x8-fiber MTP to 24-fiber LC shutteredMTP ® is a registered trademark of US Conec, Ltd.。
1989-2001几ε
PARTS INCLUDED1 P C Mishimoto Aluminum Fan Shroud3PC Mishimoto 10" Slim Electric Fans1 P C A/C Fan Adapter Harness1 P C Application-Specific W iring Harness1PC Insulating Adhesive-Backed Foam Strip1 P C Mounting Hardware PackTOOLS NEEDED7mm Socket8mm Socket10mm SocketT30Torx1/4" Drive Ratchet & Extension2.5mm Allen KeySoldering IronPliersINSTALL TIME 3 Hours INSTALL DIFFICULTY 00000 DISCLAIMER•Raise vehicle only on jack stands or on a vehicle lift.•Allow vehicle to cool completely prior to attempting installation.•Do not run the engine or drive the vehicle while overheating; serious damage can occur.•Please dispose of any liquids properly.•Mishimoto is not responsible for any vehicle damage or personal injury due to installation errors, misuse, or removal of Mishimoto products.•Mishimoto suggests that a trained professional install all Mishimoto products.INSTALL PROCEDURE1.Remove the bolts on the upper support bar.(4x T30 torx bolts)2.Remove the bolts that secure the upper supportbar. You will need to remove the battery to reachone of the covered bolts. (6x 10mm bolts)3.Unscrew the bolts that secure the radiator to theupper support bar. (2x 8mm bolts)4.Remove the bolts on the fan shroud. These canbe 10mm or 8mm. (2x 8/10mm bolts)5.Detach the metal support just behind the grille'stop edge. (4x 10mm bolts) CONTINUED ON FOLLOWING PAGE O 16.Take out the loose upper support bar.7.Separate the fan shroud connecter. Push the redtab on the side and then depress the black tab atthe center.8.Lift the AC condenser fan out from behindthe radiator.9.Remove the adjacent fan shroud. You may needto unscrew bolts on the fan clutch assembly.Replace the nuts once the fan is out.(4x 10mm nuts)10.Remove the brackets that hold the AC condenserto the radiator. (2x 8mm nuts)11.Put the push probe sensor about 3" down fromthe top of the radiator and 1" over from theheader plate.12.If you're not using a push probe sensor, you aregoing to instead install the NPT plug and sensoronto your end tank or splice it into the hose onyour cooling system. With the NPT plug, this willattach to the passenger-side end tank on yourMishimoto radiator.13.An earlier version Mishimoto radiator will requireyou to obtain an adapter by contacting MishimotoCustomer Service. The two spots where you cancut the hose and install the adapter are on eitherCONTINUED ON FOLLOWING PAGE O 2side of the compressor. Line up the adapter with the hose before making your cut to avoidremoving too much. Once the adapter is installed with worm gear clamps, wrap the NPT plug with Teflon tape and thread it into the adapter.14.Reattach the AC condenser to the radiator.(2x 8mm nuts)15.Install the fan controller unit to the back of thefan shroud in the orientation shown here.(2x 4mm bolts, 2x 4mm nuts)16.Measure 1 ½" over from the passenger side of thefan shroud and place a foam strip on the top and bottom.17.Place a longer foam strip along both thepassenger side and driver side edges.18.Lower the fan shroud behind the radiator.19.Reposition the nut clips on the upper support bar.20.Align the clips on the coolant hose so theycorrespond with the new clip positions.21.Attach the fan shroud to the upper support bar.(2x 8/10mm bolts)22.Reattach the upper support bar. (6x 10mm bolts)23.Connect the fan shroud wiring on the driver side.Use the provided jumper cable if the connectors do not match. Tuck the wiring out of the way and zip tie it to the fan.CONTINUED ON FOLLOWING PAGEO 324.Plug in the connector for the fan controller.25.At this stage in the install, you will begin thewiring setup. Use the diagram provided at the end of this guide for a reference in completing the steps that follow.26.Follow the red wire from the fan controller to thefuse. Just after the fuse, about 3" or 4", cut the red wire.27.Take the black wires coming from each fan andcut off the bullet connectors. Strip the ends so they are the same length as the red wire. Crimp together the three wires and seal the assembly with the provided end terminal connector.28.Cut the bullet connectors off the blue wire comingfrom each fan. Twist the two cut wires together.Put them inside the male spade connector and crimp the connector. Apply solder to the assembly.29.Run the provided red wire behind the battery tothe positive terminal. The other end runs to the blue wire. Cut the excess wire.30.Strip both ends of the red wire. Crimp the shownconnector onto one end. Apply solder and seal the connector using 1" of heat shrink.31.Slide 2" of heat shrink onto the opposite end.Crimp a female spade connector onto this end and apply solder. DO NOT yet seal with heat shrink.32.Attach the female and male spade connectors.Slide the heat shrink over the connection and apply heat to seal it.CONTINUED ON FOLLOWING PAGE0433.Run the black wire from the fan controller to thebattery's negative terminal. Repeat previous stepsfor attaching a connector to this wire-strip,crimp, solder, and seal the assembly.34.Take the long red wire and attach a fuse jumperto the red wire using two spade connectors. Applyheat shrink over the two spades. You can useany fuse here as long as it's a switchedignition source.35.Find an access point to get through the firewall.The easiest way to do that is to do so through thefuse box, which is in the passenger-side foot well.Ours was completed through an access point onthe driver-side firewall. Yours may be different.36.Remove the fuse box cover. Plug the fuse on thered wire into the box. Replace the fuse box cover.bine the yellow wire from the fan controllerwith the other end of the red wire. Strip the otherend of the red wire from the fuse box. Do thesame with the yellow wire coming from the fancontroller. Slide heat shrink over the yellow wire.Connect the red and yellow wires using a crimpconnector. Seal the assembly with theheat shrink.38.The green wire is optional. Tuck it away andsecure it if not being used. The green wire allowsyou to hook up to a positive battery source and itwill kick on the fans. Some builds may want tohook the green wire up to a positive switch insidethe cabin, so the fans before are activatedby temperature.39.Connect the temperature sensor. Secure anyloose wiring.40.Apply the wiring cover included in your kit. Startnearest to the fan controller and work your wayaround to the back of the engine.41.Secure the wiring so it is out of the way.42.Connect the positive line to the positive terminalon the battery, and negative line to thenegative terminal.CONTINUED ON FOLLOWING PAGE O 543.Zip tie all loose wiring to keep it from getting intothe engine.44.Reinstall the metal support just behind the grille'stop edge. (4x 10mm bolts)45.Secure the edge of the upper support bar.(4x T30 torx bolts)46.Secure the upper support bar to the radiator.(2x 8mm bolts)47.Check when the fans turn on before getting onthe road. Run the engine to get it up intemperature and make sure the fans turn on atthe right time. If the fans are not turning on at the right temperature, adjust the temperature bysimply removing the small rubber plug at theback of the fan controller and using a flat headscrewdriver to change whether they come onsooner or later in the temperature range.Congratulations! You have finishedinstalling the Mishimoto 1989-2001 JeepCherokee XJ 4.0L Fan Shroud Kit. CONTINUED ON FOLLOWING PAGE 06WIRING DIAGRAM/POSITIVE FAN WIRE TO BATTERY (+)RED -30AMP FUSE\FAN CONTROLLER YELLOW -TO SWITCHED IGNITION (12VDC)BLACK -TO CHASSIS GROUND(-)GREEN -MANUAL/ A C OVERRIDE CIRCUIT+ l 2DC07。
贝利摩G765 2.5英寸冷暖水控制阀说明书
G765•••••Technical dataFunctional dataValve Size 2.5" [65]Fluidchilled or hot water, up to 60% glycol Fluid Temp Range (water)32...350°F [0...176°C]Body Pressure Rating ANSI Class 125, up to 175 psi below 150°F Flow characteristic linearServicing repack/rebuild kits available Rangeability Sv 50:1Flow Pattern 3-way Mixing Leakage rateANSI Class III Controllable flow range stem up - open B – AB Cv 68 ANSI Class125Body pressure rating noteup to 175 psi below 150°F MaterialsValve body Cast iron - ASTM A126 Class B Valve plug bronzeStem seal NLP EPDM (no lip packing)SeatStainless steel AISI 316Pipe connection125 lb flanged Suitable actuatorsNon-Spring EVB(X)RVB(X)Electronic fail-safeAVKB(X)(2*GKB(X))Safety notesWARNING: This product can expose you to lead which is known to the State of California to cause cancer and reproductive harm. For more information go to The valve has been designed for use in stationary heating, ventilation and air-conditioning systems and must not be used outside the specified field of application, especially in aircraft or in any other airborne means of transport.Only authorised specialists may carry out installation. All applicable legal or institutional installation regulations must be complied during installation.The valve does not contain any parts that can be replaced or repaired by the user.When determining the flow rate characteristic of controlled devices, the recognised directives must be observed.Product featuresG765 Flow/Mounting detailsDimensionsDimensional drawingsEVB, EVX, RVB, RVXA B C D E F Number of Bolt Holes11.7" [298]9.0" [229]25.4" [646]18.3" [464] 3.6" [92] 3.6" [92]42*GMB, 2*GMX, 2*GKB, 2*GKXA B C D E F Number of Bolt Holes11.7" [298]9.0" [229]29.0" [736]21.8" [554] 3.5" [89] 5.3" [135]4AVKB, AVKXA B C D E F Number of Bolt Holes12.8" [325]9.0" [229]25.4" [646]18.3" [464] 3.6" [92] 3.6" [92]4AFB, AFXG765A B C D E F Number of Bolt Holes11.7" [298]9.0" [229]24.0" [610]16.9" [428] 3.5" [89] 5.3" [135]42*AFB, 2*AFXA B C D E F Number of Bolt Holes11.7" [298]9.0" [229]29.0" [736]21.8" [554] 3.5" [89] 5.3" [135]4EVB24-SR Modulating, Non-Spring Return, Linear, 24 V, forDC 2...10 V or 4...20 mATechnical dataElectrical data Nominal voltage AC/DC 24 VNominal voltage frequency50/60 HzPower consumption in operation 5 WPower consumption in rest position 1.5 WTransformer sizing7.5 VA (class 2 power source)Electrical Connection18 GA plenum cable, 3 ft [1 m], with 1/2" conduitconnector, degree of protection NEMA 2 / IP54Overload Protection electronic throughout full strokeElectrical Protection actuators are double insulatedFunctional data Actuating force motor560 lbf [2500 N]Operating range Y 2...10 VOperating range Y note 4...20 mA w/ ZG-R01 (500 Ω, 1/4 W resistor)Input Impedance100 kΩ for 2...10 V (0.1 mA), 500 Ω for 4...20 mAPosition feedback U 2...10 VDirection of motion motor selectable with switch 0/1Manual override 5 mm hex crank (3/16" Allen), suppliedStroke2" [50 mm]Running Time (Motor)90 s, constant, independent of loadRunning time motor note constant, independent of loadNoise level, motor60 dB(A)Position indication Mechanically, with pointerSafety data Degree of protection IEC/EN IP54Degree of protection NEMA/UL NEMA 2 UL Enclosure Type 2Agency Listing cULus acc. to UL60730-1A/-2-14, CAN/CSAE60730-1:02, CE acc. to 2014/30/EU and 2014/35/EU; Listed to UL 2043 - suitable for use in airplenums per Section 300.22(c) of the NEC andSection 602.2 of the IMCQuality Standard ISO 9001Ambient temperature-22...122°F [-30...50°C]Storage temperature-40...176°F [-40...80°C]Ambient humidity max. 95% r.H., non-condensingServicing maintenance-freeWeight Weight 5.73 lb [2.6 kg]Materials Housing material Die cast aluminium and plastic casingSafety notesEVB24-SR•••••PVC W'Shld for GV w/UGLK (GM)Battery Back Up System for SY(7~10)-110120 to 24 VAC, 40 VA transformer.50% voltage divider kit (resistors with wires).PC Tool computer programming interface, serial port.Electrical installationINSTALLATION NOTESActuators may also be powered by 24 VDC.A 500 Ω resistor (ZG-R01) converts the 4...20 mA control signal to 2...10 V.Actuators with plenum cable do not have numbers; use color codes instead.Meets cULus requirements without the need of an electrical ground connection.Warning! Live Electrical Components!During installation, testing, servicing and troubleshooting of this product, it may be necessary to work with live electrical components. Have a qualified licensed electrician or other individual who has been properly trained in handling live electrical components perform these tasks. Failure to follow all electricalsafety precautions when exposed to live electrical components could result in death or serious injury. VDC / 4 to 20 mA。
完美国际颜色代码表文字版
Pink 粉红#FFC0CB 255,192,203 16761035 Crimson 猩红#DC143C 220,20,60 14423100 LavenderBlush 脸红的淡紫色#FFF0F5 255,240,245 16773365 PaleVioletRed 苍白的紫罗兰红色#DB7093 219,112,147 14381203 HotPink热情的粉红#FF69B4 255,105,180 ******** DeepPink深粉色#FF1493 255,20,147 16716947 MediumVioletRed 适中的紫罗兰红色#C71585 199,21,133 ******** Orchid 兰花的紫色#DA70D6 218,112,214 14315734 Thistle 蓟#D8BFD8 216,191,216 14204888 plum 李子#DDA0DD 221,160,221 14524637 Violet 紫罗兰#EE82EE 238,130,238 15631086 Magenta 洋红#FF00FF 255,0,255 16711935 Fuchsia 灯笼海棠(紫红色) #FF00FF 255,0,255 16711935 DarkMagenta 深洋红色#8B008B 139,0,139 9109643 Purple 紫色#800080 128,0,128 8388736 MediumOrchid 适中的兰花紫#BA55D3 186,85,211 12211667 DarkViolet 深紫罗兰色#9400D3 148,0,211 9699539 DarkOrchid 深兰花紫#9932CC 153,50,204 10040012 Indigo 靛青#4B0082 75,0,130 4915330 BlueViolet 深紫罗兰的蓝色#8A2BE2 138,43,226 9055202 MediumPurple 适中的紫色#9370DB 147,112,219 9662683 MediumSlateBlue 适中的板岩暗蓝灰色#7B68EE 123,104,238 8087790 SlateBlue 板岩暗蓝灰色#6A5ACD 106,90,205 6970061 DarkSlateBlue 深岩暗蓝灰色#483D8B 72,61,139 4734347 Lavender 熏衣草花的淡紫色#E6E6FA 230,230,250 15132410 GhostWhite 幽灵的白色#F8F8FF 248,248,255 16316671 Blue 纯蓝#0000FF 0,0,255 255 MediumBlue 适中的蓝色#0000CD 0,0,205 205 MidnightBlue 午夜的蓝色#191970 25,25,112 1644912 DarkBlue 深蓝色#00008B 0,0,139 139Navy 海军蓝#000080 0,0,128 128 RoyalBlue 皇军蓝#4169E1 65,105,225 4286945 CornflowerBlue 矢车菊的蓝色#6495ED 100,149,237 6591981 LightSteelBlue 淡钢蓝#B0C4DE 176,196,222 11584734 LightSlateGray 浅石板灰#778899 119,136,153 7833753 SlateGray 石板灰#708090 112,128,144 7372944 DodgerBlue 道奇蓝#1E90FF 30,144,255 2003199AliceBlue 爱丽丝蓝#F0F8FF 240,248,255 15792383SteelBlue 钢蓝#4682B4 70,130,180 4620980 LightSkyBlue 淡天蓝色#87CEFA 135,206,250 8900346 SkyBlue 天蓝色#87CEEB 135,206,235 8900331 DeepSkyBlue 深天蓝#00BFFF 0,191,255 49151 LightBLue 淡蓝#ADD8E6 173,216,230 11393254 PowDerBlue 火药蓝#B0E0E6 176,224,230 11591910 CadetBlue 军校蓝#5F9EA0 95,158,160 6266528 Azure 蔚蓝色#F0FFFF 240,255,255 15794175 LightCyan 淡青色#E1FFFF 225,255,255 14745599 PaleTurquoise 苍白的绿宝石#AFEEEE 175,238,238 11529966 Cyan 青色#00FFFF 0,255,255 65535 Aqua 水绿色#00FFFF 0,255,255 65535 DarkTurquoise 深绿宝石#00CED1 0,206,209 52945 DarkSlateGray 深石板灰#2F4F4F 47,79,79 3100495 DarkCyan 深青色#008B8B 0,139,139 35723 Teal 水鸭色#008080 0,128,128 32896 MediumTurquoise 适中的绿宝石#48D1CC 72,209,204 477230032,178,170 2142890 LightSeaGreen 浅海洋绿B2AATurquoise 绿宝石#40E0D0 64,224,208 4251856 Aquamarine 绿玉,碧绿色#7FFFAA 127,255,170 8388564 MediumAquamarine 适中的碧绿色#66CDAA 102,205,170 6737322 MediumSpringGreen 适中的春天的绿色#00FA9A 0,250,154 64154 MintCream 薄荷奶油#F5FFFA 245,255,250 16121850 SpringGreen 春天的绿色#00FF7F 0,255,127 65407 MediumSeaGreen 适中的海洋绿#3CB371 46,139,87 3978097 SeaGreen 海洋绿#2E8B57 46,139,87 3050327 Honeydew 蜂蜜#F0FFF0 240,255,240 15794160 LightGreen 淡绿色#90EE90 144,238,144 9498256 PaleGreen 苍白的绿色#98FB98 152,251,152 ******** DarkSeaGreen 深海洋绿#8FBC8F 143,188,143 9419919 LimeGreen 酸橙绿#32CD32 50,205,50 3329330 Lime 酸橙色#00FF00 0,255,0 65280 ForestGreen 森林绿#228B22 34,139,34 2263842 Green 纯绿#008000 0,128,0 32768 DarkGreen 深绿色#006400 0,100,0 25600 Chartreuse 查特酒绿#7FFF00 127,255,0 8388352 LawnGreen 草坪绿#7CFC00 124,252,0 8190976 GreenYellow 绿黄色#ADFF2F 173,255,47 11403055 DarkOliveGreen 深橄榄绿#556B2F 85,107,47 5597999OliveDrab 橄榄土褐色#556B2F 85,107,47 7048739 Beige 米色(浅褐色) #6B8E23 107,142,35 16119260 LightGoldenrodYellow 浅秋麒麟黄#FAFAD2 250,250,210 16448210 Ivory 象牙#FFFFF0 255,255,240 16777200 LightYellow 浅黄色#FFFFE0 255,255,224 16777184 Yellow 纯黄#FFFF00 255,255,0 16776960 Olive 橄榄#808000 128,128,0 7421376 DarkKhaki 深卡其布#BDB76B 189,183,107 12433259 LemonChiffon 柠檬薄纱#FFFACD 255,250,205 16775885 PaleGoldenrod 灰秋麒麟#EEE8AA 238,232,170 15657130 Khaki 卡其布#F0E68C 240,230,140 15787660 Gold 金#FFD700 255,215,0 16766720 Cornsilk 玉米色#FFF8DC 255,248,220 16775388 GoldEnrod 秋麒麟#DAA520 218,165,32 14329120 DarkGoldEnrod 深秋麒麟#B8860B 184,134,11 12092939 FloralWhite 花的白色#FFFAF0 255,250,240 16775920 OldLace 老饰带#FDF5E6 253,245,230 16643558 Wheat 小麦色#F5DEB3 245,222,179 16113331 Moccasin 鹿皮鞋#FFE4B5 255,228,181 ******** Orange 橙色#FFA500 255,165,0 16753920 PapayaWhip 番木瓜#FFEFD5 255,239,213 16773077 BlanchedAlmond 漂白的杏仁#FFEBCD 255,235,205 16772045 NavajoWhite Navajo白#FFDEAD 255,222,173 ******** AntiqueWhite 古代的白色#FAEBD7 250,235,215 16768685 Tan 晒黑#D2B48C 210,180,140 13808780 BurlyWood 结实的树#DEB887 222,184,135 ******** Bisque (浓汤)乳脂,番茄等#FFE4C4 255,228,196 16770244 DarkOrange 深橙色#FF8C00 255,140,0 16747520 Linen 亚麻布#FAF0E6 250,240,230 16445670 Peru 秘鲁#CD853F 205,133,63 13468991 PeachPuff 桃色#FFDAB9 255,218,185 ******** SandyBrown 沙棕色#F4A460 244,164,96 16032864 Chocolate 巧克力#D2691E 210,105,30 13789470 SaddleBrown 马鞍棕色#8B4513 139,69,19 9127187 SeaShell 海贝壳#FFF5EE 255,245,238 16774638 Sienna 黄土赭色#A0522D 160,82,45 10506797 LightSalmon 浅鲜肉(鲑鱼)色#FFA07A 255,160,122 16752762Coral 珊瑚#FF7F50 255,127,80 16744272DarkSalmon 深鲜肉(鲑鱼)色#E9967A 233,150,122 15308410 Tomato 番茄#FF6347 255,99,71 16737095 MistyRose 薄雾玫瑰#FFE4E1 255,228,225 16770273 Salmon 鲜肉(鲑鱼)色#FA8072 250,128,114 16416882 Snow 雪#FFFAFA 255,250,250 16775930 LightCoral 淡珊瑚色#F08080 240,128,128 15761536 RosyBrown 玫瑰棕色#BC8F8F 188,143,143 12357519 IndianRed 印度红#CD5C5C 205,92,92 13458524 Red 纯红#FF0000 255,0,0 16711680 Brown 棕色#A52A2A 165,42,42 10824234 FireBrick耐火砖#B22222 178,34,34 11674146 DarkRed 深红色#8B0000 139,0,0 9109504 Maroon 栗色#800000 128,0,0 8388608 White 纯白#FFFFFF 255,255,255 16777215 WhiteSmoke 白烟#F5F5F5 245,245,245 16119285 Gainsboro Gainsboro #DCDCDC 220,220,220 14474460 LightGrey浅灰色#D3D3D3 211,211,211 13882323 Silver 银白色#C0C0C0 192,192,192 12632256 DarkGray深灰色#A9A9A9 169,169,169 11119017 Gray 灰色#808080 128,128,128 8421504 DimGray暗淡的灰色#696969 105,105,105 6908265Black 纯黑#000000 0,0,0 0。
Lattice Boom Crawler Crane LS–138H II 80-ton (72.5
Litho in USA 3/02#5349 (supersedes #5296)SpecificationsLattice Boom Crawler CraneLS–138H II80–ton (72.57 metric ton)HYLAB Series-2-LS–138H II LS–138H II Machine Transport Weights – approximateLS–138H II–3–LS–138H II Transportation Weights – approximateBase Machine: Rigid Boom Backstops, 27 Gallons (102.2 L) of Fuel, Catwalks (front right and left side), 20ʼ(6.10 m)Tube Base Section, 24ʼ(6.10m) Live Mast,Bridle & Spreader Bar, 14–Part Boom Hoist Reeving, 700ʼ(189 m) of Type ʻDB ʼ Front Hoist Rope, 540ʼ (165m ) of Type ʻRB ʼ Rear Hoist Rope.Gross WeightTransport LoadsItem Descriptionlbs.kgLoad #1Load #2Load #3Notes and Load SummaryBase Machine89,75140 7101Numbers in the load columns to the left represent quantities.Add “A ” Counterweight 25,25011 4531Add “B ” Counterweight25,25011 4531Estimated transport loadAdd Hydraulic Third Drum w/o rope 1,345610assumes the load out consist of Add 20ʼ(6.1m) Tube Top Section2,726 1 2371200ʼ(60.96 m) of tube boom +60ʼ (18.29 m ) of jib with full Add 10ʼ (3.05m ) Tubular Extension w/pins & pendants 6773071counterweight.Add 20ʼ (6.1m ) Tubular Extension w/pins & pendants 1,07648812Support loads were targeted at Add 30ʼ (9.1m ) Tubular Extension w/pins & pendants 1,48167221Add 20ʼ (6.1m ) Angle Base Section at 0 degrees2,853 1 29445,000 lb (20 412 kg ), 8ʼ–6”(2.6 m ) wide, 48ʼ (14.63 m ) long,Add 20ʼ (6.1m ) Angle Top Section with 4 Lifting Sheaves 3,5001 588and 13ʼ–6” (4.11 m ) high using a Add 20ʼ (6.1m ) Angle Top Section with 3 Lifting Sheaves 3,400 1 592drop deck trailer. This may vary Add 20ʼ (6.1m ) Angle Top Section with2 Lifting Sheaves 3,300 1 497depending on state laws, empty truck/trailer weights, and style Add 10ʼ (3.05m ) Angular Extension w/pins & pendants 1,040472of trailer.Add 20ʼ (6.1m ) Angular Extension w/pins & pendants 1,680762Add 30ʼ (9.1m ) Angular Extension w/pins & pendants 2,400 1 089Estimated weights vary by Add Bridle & Spreader Bar Only (No Live Mast)990449+/– 2%.Add Tagline Winder 760345Estimated Total Load of #1Add Fairleader 50022789,751 lbs. (40 710 kg).Add PAT DS–35010045Estimated Total Load of #2Add 30ʼ (9.1m ) Tubular Jib1,6837631Add 15ʼ (4.6m ) Tubular Jib Extension 317144235,089 lbs. (15 916 kg).Add 5ʼ (1.5m ) Auxiliary Tip Extension800363Estimated Total Load of #3Add Holding Rope – 0.88” X 165ʼ Type ʻDB ʼ23410631,877 lbs. (14 459 kg).Add Closing Rope – 0.88” X 220ʼ Type ʻDB ʼ312142Add Inhaul Rope – 0.88” X 80ʼ Type ʻM ʼ10849Add Hoist Rope – 0.88” x 165ʼ Type ʻDB ʼ234106Add Jib Wire Rope – 0.88” X 700ʼ Type ʻDB ʼ994451Add 3rd Drum Wire Rope 0.63” X 385ʼ Type ʻZB ʼ312142Add 3rd Drum Wire Rope 0.63” X 385ʼ Type ʻWB ʼ296134Add Auxiliary Lifting Bail20091Add 15–ton (13.6mt) Hook Ball – Non Swivel 7503401Add 15–ton (13.6mt ) Hook Ball – Swivel 760345Add 80–ton (72.6mt ) 4 Sheave Hook Block 2,325 1 0551Remove 20ʼ Tube Base Section–1,988–902Remove Front Hoist Rope 0.88” X 700ʼ Type ʻDB ʼ–944–451Remove Jib Wire Rope 0.88” X 540ʼ Type ʻRB ʼ–810–367Remove 24ʼ (7.3m ) Live Mast with Bridle & Spreader Bar –2,618–1 188Add 50 gallons (189.3L ) of Fuel362164Machine Working WeightOptionDescriptionGross Weight lbs. (kg )Ground BearingPressure psi (kg/cm 2)1Base Machine equipped with 40ʼ(12.2 m ) of tubular boom, live mast, “A ” counterweight, 700ʼ(213 m) front hoist rope, 540ʼ(164.6 m) rear hoist rope, 80–ton (72.6 mt) hook block, 77 gallons (291.4 L) of fuel, and a 200 lbs. (90.7kg) operator.121,097(54 929)7.71(0.54)2Option #1 plus “B ” counterweight, midpoint pendants, and 160ʼ(48.77m)of boom extensions to obtain 200ʼ(60.96m) of main boom.155,627(70 591)9.92(0.70)3Option #2 plus 60ʼ(18.29 m) of jib and 15–ton (13.61 mt) hookball – subtract 20ʼ (6.10 m) of boom extension and midpoint pendants to obtain maximum 180ʼ + 60ʼ(54.86 + 18.29 m) of main boom + jib.157,452(71 419)10.03(0.70)4Base Machine equipped with 40ʼ(12.20 m) of angle boom, live mast, “A ” counterweight, 700ʼ(213 m) front hoist rope, 540ʼ(164.59 m) rear hoist rope, 80–ton (72.57 mt) hook block, 77 gallons (291.4 L) of fuel, and a 200 lbs. (90.7kg) operator.121,722(55 212)7.76(0.54)5Option #4 plus “B ” counterweight and 110ʼ(33.55 m) of boom extensions to obtain 150ʼ (45.72 m ) of main boom.156,172(70 838)9.95(0.71)6Option #5 plus 60ʼ (18.29 m) of jib and 15–ton (13.61 mt ) hookball to obtain maximum 150ʼ + 60ʼ(45.72 + 18.29 m) of main boom + jib.155,387(70 482)9.90(0.70)Notes:1.Ground bearing pressure is based on the total weight distributed evenly over the track contact area.2.Total contact area for 36”(0.91m ) track shoes is 15,689 in 2(101,219cm 2).-4-LS–138H II Attachment OptionsJ 40ʼ–200ʼ Tube Boom (12.19 – 60.96 m) Basic Tube Boom – 40ʼ(12.19 m) two–piece design that utilizes a 20ʼ(6.10 m)base section and a 20ʼ(6.10 m) open throat top section with in–line connecting pins on 54”(1.37 m) wide and 44”(1.12m) deep centers.S Boom feet on 50”(1.27 m) centers S 3”(76.2 mm) diameter chordsS Lugs on base section to attach carrying linksS Skywalk platformS Deflector roller on top sectionS Permanent skid pads mounted on top section to protect head machinery S Rigid sheave guardsS Five 18”(0.46 m) root diameter steel sheaves mounted on sealed anti–fric-tion bearingsS Mechanical boom angle indicatorOptional – Handling system that mounts in the boom base to allow loading/un-loading of a counterweight or a boomsection onto transport trailers.Tube Boom Extensions – The following table provides the lengths available andthe suggested quantity to obtain maxi-mum boom in 10ʼ(3.05 m) increments.Midpoint pendant connections are re-quired at 80ʼ(24.38 m) for 190ʼ (57.91 m)and 200ʼ(60.96 m ) boom lengths.Tube Boom Extensions Suggested Quantity forMax. Boom10ʼ (3.05 m )120ʼ (6.10 m )130ʼ (9.14 m )4S Deflector roller on top of each sectionS Appropriate length pendantsS Maximum tube boom tip height of 204ʼ(62.18 m)J 40ʼ–150ʼ Angle Boom (12.19 – 45.72 m)Basic Angle Boom – 40ʼ(12.19 m) two–piece design that utilizes a 20ʼ(6.10 m)base section and a 20ʼ(6.10 m) open throat top section with in–line connecting pins. Boom extensions are 48”(1.22 m)wide and 48”(1.22 m) deep at outside dimensions of angles.S Boom feet on 50”(1.27 m) centersS 4” X 4” X 0.38”(101.6 x 101.6 x 9.5 mm)angle chordsS Lugs on base section to attach carrying linksS Skywalk platformS Deflector roller on top sectionS Permanent skid pads mounted on top section to protect head machinery S Rigid sheave guardsS Four 18”(0.46 m) root diameter steel sheaves mounted on sealed anti–friction bearings S Mechanical boom angle indicatorOptional – Three sheave head machin-ery for clam applications or two widesheaves for dragline appplications Angle Boom Extensions – The follow-ing table provides the lengths availableand the suggested quantity to obtainmaximum boom in 10ʼ(3.05 m) incre-ments. Midpoint pendant connectionsare not required.Angle Boom Extensions Suggested Quantity for Max. Boom10ʼ (3.05 m )120ʼ (6.10 m )230ʼ (9.14 m )2S Deflector roller on top of each section S Appropriate length pendantsS Maximum angle boom tip height of 154ʼ(46.94 m)J 30ʼ– 60ʼ Tube Jib(9.14– 18.29 m)Basic Tube Jib – 30ʼ(9.14 m) two–piecedesign that utilizes a 15ʼ(4.57 m) base section and a 15ʼ (4.57 m ) top section with in–line connecting pins on 32”(0.81m) wide and 24” (0.61 m ) deep centers.S 2”(50.8 mm) diameter tubular chords S One 18.5”(0.47 m) root diameter steel sheave mounted on sealed anti–friction bearings.S 15ʼ (0.38 mm) jib extensions provide jib lengths at 45ʼ(13.76 m ) and 60ʼ(18.29m)S Jib offset angles at 5, 15 and 25 degrees S Maximum tip height of boom + jib is 242ʼ(73.76 m) using the tube boom and 204ʼ(65.23 m) using the angle boomJ Auxiliary 5ʼ (1.52 m)Tip ExtensionDesigned to use instead of a jib to pro-vide clearance between working hoist lines. The extension is equipped with a single 15.25”(0.39 m) root diameter steel sheave mounted on sealed anti–friction bearings. Maximum capacity is 9–ton (8.16 mt).J Boom Hoist SystemDesigned to lift off maximum boom or maximum boom plus jib unassisted.Operates up to a maximum boom angleof 82 degrees. Automatically limits maxi-mum boom angle operation.S Retractable gantry frameS Pin–on bail frame S 14–part reeving with 5/8”(14.7 mm)type ʻW ʼ wire ropeS Bridle assemblyS 24ʼ(7.31 m) live mast (optional for angle attachment)S Two 1.25”(31.75 mm) pendants S Telescopic boom backstops (tubular-type)S Sheaves contain sealed anti–friction bearingsS Boom speed from 10_–70_ is 52 sec-onds with no load and 94 seconds withfull load. Speed was determined using 100ʼ(30.5 m ) of tube boomLS–138H II–5–Revolving UpperstructureJ FrameAll welded steel frame with precision ma-chined surfaces for mating parts.J Engine Isuzu A–6SDITQB with oil cooler, oil cooler, air cleaner, fuel filter, water separator, hour meter,tachometer, and electrical shutdown.Number of cylinders6Bore and stroke – in(mm )4.72 x5.71 (120 x 145)Piston displacement – in 3 (cm 3)600(9 839)Engine rpm at full load speed 2,100Hi–idle rpm2,325Full load speed – horsepower (kw )207(155)Peak torque – ft lb (joule )513(696)Peak torque – rpm 1,400Electrical system 24 volt Batteries 2–12 volt Approximate fuel consumptionGal./hr (L/hr)100% H.P .11.50 (43.53)75% H.P .8.60(32.55)50% H.P . 5.75(21.77)25% H.P .2.87(10.86)J Hydraulic SystemHydraulic Pumps – The pump arrange-ment is designed to provide hydraulically powered functions allowing positive, pre-cise control with independent or simulta-neous operation of all crane functions.S Two variable displacement pumps op-erating at 4,000 psi (281.24 kg/cm 2) and 64 gal/min (243 l/min ) powers load hoist drums, boom hoist drum, optional third drum, and travel.S One fixed displacement gear type pump operating at 3,600 psi (250 kg/cm 2) and 32 gal/min (121 l/min ) powers the swing,and treadmember retract cylinders.S One fixed displacement gear type pump operating at 1,250 psi (85 kg/cm 2) and 8gal/min (32 l/min) powers the pilot con-trol system, clutches, brakes, pump controls, counterweight removal sys-tem, and optional handling system mounted in boom base.Pump Control (“Fine Inching”) mode Special pump setting, selectable from operator ʼs cab, that allows very slow movements of load hoist drums, boom hoist drum, and travel for precision work.Hydraulic Reservoir – 78 US gallons (295 l), equipped with sight level gauge.Diffusers built in for deaeriation.Filtration – One 10 micron, full flow, line filter in the control circuit. All oil is filteredprior to entering the reservoir.Counterbalance Valves – All hoistmotors are equipped with counterbal-ance valves to provide positive load low-ering and prevent accidental load drop ifthe hydraulic pressure is suddenly lost.J Load Hoist DrumsEach drum contains a pilot controlled,bi–directional, axial piston motor and a planetary gear reduction unit to provide positive control under all load conditions.S Power up/down & free–fall operation modesS Automatic brake mode (spring applied,hydraulically released, band type brake)S 0.88”(22.22 mm) grooved lagging S Drum pawl controlled manually S Electronic drum rotation indicators S Mounted on anti–friction bearings S 17.64”(0.45 m) root diameter S 29.92”(0.76 m) flange diameter S 19.84” (0.50 m) widthNote: The freefall operational mode is designed to prevent load lowering even if the freefall switch is accidentally acti-vated. The automatic brake mode meets all OSHA requirements for personnel handling.Drum Clutches – Speed–o–Matic t power hydraulic two shoe clutch design that uses a 20”(0.51 mm) diameter x 5”(127 mm ) wide shoe that internally ex-pands to provide load control. Swept area is 314 in 2 (2 026 cm 2).J Optional Front Mounted Third Hoist Drum The hydraulic winch is pinned to the frontof the upper frame and is used in con-junction with a fleeting sheave and3–sheave idler assembly to run the wirerope over the boom top section.S Free–spooling capability for piledriving applicationsS 10.63”(0.27 m) root diameterS 20”(0.51 m) outside flange diameterS 13.5” (0.34 m) widthS Mounted on anti–friction bearingsJ Boom Hoist Drum Contains a pilot controlled, bi–directional,axial piston motor and a planetary gear reduction unit to provide positive control under all load conditions.S Spring applied, hydraulically released,disc type brake controlled automatically S 5/8”(15.88 mm ) grooved laggingS Drum pawl controlled manuallyS Mounted on anti–friction bearingsS 12.60”(0.31 m ) root diameter S 24.41”(0.62 m ) flange diameterS 9.57”(0.24 m ) widthJ Swing SystemMechanical linkage controls the bi–direc-tional axial piston motor and the plane-tary gear reduction unit to provide posi-tive control under all load conditions.S Spring applied, hydraulically released,360 degree multi–plate brakeS Free swing mode when lever is in neu-tral positionS Two position positive house lock S Audio/Visual swing alarmS Maximum swing speed is 2.8 rpmJ Upper CounterweightConsist of a two piece design that can be easily lowered to the ground using the gantry.S 25,250 lbs. (11 453 kg)“A ” upper counterweight S 25,250 lbs. (11 453 kg)“B ” uppercounterweight can be added to maximize capacities J Operator ʼs Cab andControlsFully enclosed modular steel compart-ment is independently mounted and in-sulated to protect against vibration and noise.S All tinted/tempered safety glassS Sliding entry door and front windowS Swing up roof window with wiperS Door and window locks S Heater with circulating fanS Air conditionerS Sun visorS Engine instrumentation panel (tachome-ter, voltmeter, engine oil pressure, en-gine water temperature, fuel level, hy-draulic oil temperature, and servicemonitor system)S Mechanical drum rotation indicatorsS Six way adjustable seatS Dry chemical fire extinguisherS Hand and foot throttleS Single axis, armchair control leversS Swing lever with swing brake and horn located on handle S Bubble type level (continued on page 7)-6-LS–138H II LS–138H II Load Hoisting PerformanceAvailable line speed and line pull – based on Isuzu A–6SDITQB at 2,000 rpm full load speed. Line pulls are not based on wire rope strength. See wire rope capacity chart for maximum permissible single part of line working loads.Front or Rear Drum – 7/8”(22.22 mm) Wire RopeRope Maximum Line Pull No Load Line Speed Full Load Line Speed Pitch Diameter Layer TotalLayer lbs.kg ft/min.m/min ft/min.m/min in.mm ft.m ft.m 132,43014 710298911133518.54701003010030229,63013 440326991243820.35161093320964327,27412 3723541081354122.055911936327100425,26611 4613821161454423.860512839455139523,53310 6744101251564825.564813742593181622,0239 9894381341675127.3693147457402257––––––29.073715648896273Boom Hoist Drum – 5/8”(15.88 mm) Wire RopeRope Maximum Line Pull No Load Line Speed Full Load Line Speed Pitch Diameter LayerTotalLayer lbs.kg ft/min.m/min ft/min.m/min in.mm ft.m ft.m 117 8328 089196601093313.233648154815216 2827 385214651193614.5368521610031314 979 6 794233711304015.7400571715748413 869 6 291251771404317.0432611921867512 913 5 857270821514618.3464662028487611 080 5 479289881614919.54967021355108711 348 5 147307941715220.85287523430131810 6994 853326991825522.05608024509155Optional Third Drum – 5/8” (15.88 mm) Wire RopeRope Maximum Line Pull No Load Line Speed Full Load Line Speed Pitch Diameter LayerTotalLayer lbs.kg ft/min.m/min ft/min.m/min in.mm ft.m ft.m 115,041 6 822157481434411.2528657175717213,537 6 140175531594812.50318642012137312,307 5 582192591755313.75349712219259411,282 5 117210641915815.00381772326982510,414 4 724228692076316.25413832535210769,6714 387245752236817.504459027442135Diameter LengthMaximum Permissible LoadWire Rope Application in mm ft m Type lb kg Boom Hoist 5/815.88610186W 11,770 5 339Front Hoist7/822.22700213DB 22,74010 315Rear Hoist (Optional)7/822.22540165RB 17,5207 947Rear Hoist (Optional)7/822.22700213DB 22,74010 315Third Drum (Optional)5/815.88385117ZB 11,080 5 026Third Drum (Optional)5/815.88385117WB13,6506 192Rope Type DescriptionDB 6 x 26 (6 X 19 Class) – Warrington Seale – Extra Improved Plow Steel – Preformed – Right Lay – Regular Lay – I.W.R.C.RB*18 x 19 (19 x 19 Class) – Rotation Resistant – Extra Improved Plow Steel – Preformed – Right Lay – Regular Lay – Swaged – SF=5.1ZB 36 x 7 – Non–rotating – Extra Improved Plow Steel – Right Lay – Regular Lay – S.F.=5.1WB 8 Strand – Regular LayW6 x 26 (6 x 19 Class) – Warrington Seale – Extra Improved Plow Steel – Preformed – Right Lay – Alternate Lay*– Use of swivel ball is not recommended.LS–138H II–7–Revolving Upperstructure(continued from page 5)J Load Indicator/Rated Capacity LimiterStandard Equipment – PAT EI–65 load indicator provides two lineriders, angle sensor, computer, display, and anti–two block equipment to provide the following information.S Boom length & angle.S Jib length & angle.S Load on hook.S Load radius.S Tip height.S Anti–two block warning & function limiters.S Operation mode.S Operator settable alarms provide audio/visual warning.Optional Equipment – PAT DS–350rated capacity limiter provides all the same equipment and features of the standard El–65 in conjunction with the following features.S Provides an audio/visual warning when the load on hook is within 90% of the cranes rated load.S Provides an audio/visual warning and limits functions when the load on hook is at 100% of the cranes rated load.Note: The DS–350 function limiters areactivated for anti–two block and overloadconditions. These limiters are designedto prevent hoist up on front and reardrums and boom down.J Additional Equipment–StandardS 57.88”(1.47 m ) outside diameter turntable bearing.S Front, right, & left side removable catwalks.S 77 US Gallons (291.5 L ) fuel tank (usable quantity).S Machine lifting links.J Additional Equipment–Optional S Rud–o–matic R model 1248 taglinewinder for angle boom (double barrel,spring wound, drum type).S Rud–o–matic R model 648 tagline winder for tube boom.S Full revolving type Fairleader with barrel, sheaves, and guide rollers.Lower StructureJ Lower FrameAll welded box construction frame with precision–machined surfaces for turnta-ble bearing and rotating joint.S 8ʼ–10.7” (2.71 m ) overall width.S 11ʼ–11” (3.63 m ) overall length.J TreadmembersAll welded, precision–machined, steel frames can be hydraulically extended and retracted by a hydraulic cylinder mounted in the lower frame.S 14ʼ (4.27 m ) extended gauge.S 8ʼ–11” (2.72 m ) retracted gauge.S 20ʼ–2” (6.15 m ) overall length.S 36” (0.91 m ) wide track shoes.S 11 sealed (oil filled) track rollers per treadmember.S Sealed (oil filled) idler and drive planetaries.S Compact travel drives.S Hydraulic self adjusting tracks.Travel and Steering – Each treadmem-ber contains a pilot controlled, bi–direc-tional, axial piston motor and a planetary gear reduction unit to provide positive control under all load conditions.S Individual control provides smooth,precise maneuverability including full-counter–rotation.S Spring applied, hydraulically released disc type brake controlled automati-cally.S Maximum travel speed is 1.0 mph (1.7 km/h ) in high speed and 0.6 mph (1 km/h ) in low speed.SDesigned to 30% gradeability.-8-LS–138H II Link–Belt Construction Equipment CompanyLexington, Kentucky RLink–Belt is a registered trademark. Copyright 2002. All rights reserved. We are constantly improving our products and therefore reserve the right to change designs and specifications.。
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Grace ™SoftwareProduct bulletinFully harness MSP430 MCUs integrated analog and digital peripherals with Grace Software Enable and configure ADCs, DACs, timers, clocks, serial communication interfaces and more, by interacting with buttons, drop-down menus, and text fields. Navigate through the MSP430 MCUs highly integrated peripheral set like a pro.Grace software supports all MSP430F2xx and G2xx Value Line MCU devicesThis means it supports the MSP430 MCUs most popular tools such as MSP-EXP430G2 LaunchPad, eZ430-F2013, eZ430-RF2500 and other tool kits.Seamless integration into TI’s eclipse-based Code Composer Studio ™ softwareGrace software generates easy-to-understand C code, which is directly inserted into your active project. This seamless integration into the integrated development environment (IDE) allows the Grace generated code to be debugged and downloaded into your MSP430 microcontroller just as if it was handwritten.Grace software is now also available as a separate install. This means Grace-generated code can be easily imported into other development tools.Graphical User Interface for enablingand configuring MSP430™ MCU peripherals• Now included in Code ComposerStudio ™IDE version 5• Also available as a stand-alone tool for other IDE support• Supports all MSP430F2xx and G2xx value line devices• Enables graphical configuration of MSP430 peripherals including: – ADCs, OpAmps, timers, clocks, comparators, serial communication modules and more!• Basic user, power user and register views are offered• Fully commented, easy-to-understand and editable C code is generatedKey featuresSLAT127AA042210Important Notice: The products and services of Texas Instruments Incorporated and its subsidiaries described herein are sold subject to TI’s standard terms andconditions of sale. Customers are advised to obtain the most current and complete information about TI products and services before placing orders. TI assumes no liability for applications assistance, customer’s applications or product designs, software performance, or infringement of patents. The publication of information regarding any other company’s products or services does not constitute TI’s approval, warranty or endorsement thereof.© 2012 Texas Instruments IncorporatedThe platform bar, Grace, MSP430 and Code Composer Studio are trademarks of Texas Instruments. All other trademarks are the property of their respective owners.Fly high above the bits and bytes of low level register settingsGrace software provides various levels of abstraction, which allows users to focus more on the application layer than low-level peripheral configuration. This enables developers to create high quality, robust solutions in less time. Grace software includes a Power User, Basic User, and Register views for each peripheral, providing different levels of abstraction depending on your needs. Changes made in any setting in one view are automatically reflected in the other views.Generate easy-to-understand C code The peripheral initialization code that Grace soft-ware produces is fully commented, readable and editable. Accomplish what used to take hours, and start working on your application layer within minutes of starting up Grace software.Integrated tool tips guide peripheral setup Grace software is based directly from the MSP430 MCU user’s guides and datasheets. As users explore the various views of Grace software, developers learn about the ins and outs of the MSP430 MCU s integrated peripherals. U sers are invited to hover over interactiveelements to get detailed information about the MSP430 MCU being programmed.For more information visit, www. /graceGet started quickly and learn as you go As developers interact with Grace software, instant notifications ensure that the enabled MSP430 MCU peripherals are being properly configured. Helpful hints and popups point out errors, which prevents erroneous settings, conflicting configurations and peripheral collisions.This immediate feedback saves hours of development time,and quickens your time to market!IMPORTANT NOTICETexas Instruments Incorporated and its subsidiaries(TI)reserve the right to make corrections,modifications,enhancements,improvements, and other changes to its products and services at any time and to discontinue any product or service without notice.Customers should obtain the latest relevant information before placing orders and should verify that such information is current and complete.All products are sold subject to TI’s terms and conditions of sale supplied at the time of order acknowledgment.TI warrants performance of its hardware products to the specifications applicable at the time of sale in accordance with TI’s standard warranty.Testing and other quality control techniques are used to the extent TI deems necessary to support this warranty.Except where mandated by government requirements,testing of all parameters of each product is not necessarily performed.TI assumes no liability for applications assistance or customer product design.Customers are responsible for their products and applications using TI components.To minimize the risks associated with customer products and applications,customers should provide adequate design and operating safeguards.TI does not warrant or represent that any license,either express or implied,is granted under any TI patent right,copyright,mask work right, or other TI intellectual property right relating to any combination,machine,or process in which TI products or services are rmation published by TI regarding third-party products or services does not constitute a license from TI to use such products or services or a warranty or endorsement e of such information may require a license from a third party under the patents or other intellectual property of the third party,or a license from TI under the patents or other intellectual property of TI.Reproduction of TI information in TI data books or data sheets is permissible only if reproduction is without alteration and is accompanied by all associated warranties,conditions,limitations,and notices.Reproduction of this information with alteration is an unfair and deceptive business practice.TI is not responsible or liable for such altered rmation of third parties may be subject to additional restrictions.Resale of TI products or services with statements different from or beyond the parameters stated by TI for that product or service voids all express and any implied warranties for the associated TI product or service and is an unfair and deceptive business practice.TI is not responsible or liable for any such statements.TI products are not authorized for use in safety-critical applications(such as life support)where a failure of the TI product would reasonably be expected to cause severe personal injury or death,unless officers of the parties have executed an agreement specifically governing such use.Buyers represent that they have all necessary expertise in the safety and regulatory ramifications of their applications,and acknowledge and agree that they are solely responsible for all legal,regulatory and safety-related requirements concerning their products and any use of TI products in such safety-critical applications,notwithstanding any applications-related information or support that may be provided by TI.Further,Buyers must fully indemnify TI and its representatives against any damages arising out of the use of TI products in such safety-critical applications.TI products are neither designed nor intended for use in military/aerospace applications or environments unless the TI products are specifically designated by TI as military-grade or"enhanced plastic."Only products designated by TI as military-grade meet military specifications.Buyers acknowledge and agree that any such use of TI products which TI has not designated as military-grade is solely at the Buyer's risk,and that they are solely responsible for compliance with all legal and regulatory requirements in connection with such use. TI products are neither designed nor intended for use in automotive applications or environments unless the specific TI products are designated by TI as compliant with ISO/TS16949requirements.Buyers acknowledge and agree that,if they use any non-designated products in automotive applications,TI will not be responsible for any failure to meet such requirements.Following are URLs where you can obtain information on other Texas Instruments products and application solutions:Products ApplicationsAudio /audio Automotive and Transportation /automotiveAmplifiers Communications and Telecom /communicationsData Converters Computers and Peripherals /computersDLP®Products Consumer Electronics /consumer-appsDSP Energy and Lighting /energyClocks and Timers /clocks Industrial /industrialInterface Medical /medicalLogic Security /securityPower Mgmt Space,Avionics and Defense /space-avionics-defense Microcontrollers Video and Imaging /videoRFID OMAP Mobile Processors /omapWireless Connectivity /wirelessconnectivityTI E2E Community Home Page Mailing Address:Texas Instruments,Post Office Box655303,Dallas,Texas75265Copyright©2012,Texas Instruments Incorporated。