Method of Surface Modification by Proteins for Ana
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发明人：Petr Novak, Volny,Petr Pompach,Viktor Ruzicka
申请号：US15511680 申请日：20150916 公开号：US2017024 2030A1 公开日：20170824
专利附图：
摘要：A method for modification of solid substrates with proteins for efficient surface preconcentration of an analyte from multi-component samples before the detection based on desorption-ionization mass spectrometry and immunochemical assays. The claimed subject is a method of modification of surfaces used as substrates for desorption-ionization mass spectrometry and immuno-chemical assays. The method is based on electronebulization (electrospraying) of protein solution, depending on the intended application either enzymes, lectins, or antibodies. The formed charged electrospray is dried in real time by its passing through an evaporation compartment and the resulting beam of desolvated ions impacts onto the surface and binds to it firmly. Such modified surface can be then used for a selective interaction with an affinity partner of the deposited protein, its preconcentration or enzymatic modification followed by an analysis by means of desorption-ionization mass spectrometry or immunochemical assays.
专利内容由知识产权出版社提供
专利名称：Method of Surface Modification by Proteins for Analyte Preconcentration for Desorption-Ionization Mass Spectrometry Techniques and for Immunochemical Assays
申请人：Petr Novak,Michael Volny,Petr Pompach,Viktor Ruzicka
地址：Dolni Brezany CZ,Praha 4 CZ,Mratin CZ,Rymarov CZ
国籍：CZ,CZ,CZ,CZ
更多信息请下载全文后查看
申请号：US15511680 申请日：20150916 公开号：US2017024 2030A1 公开日：20170824
专利附图：
摘要：A method for modification of solid substrates with proteins for efficient surface preconcentration of an analyte from multi-component samples before the detection based on desorption-ionization mass spectrometry and immunochemical assays. The claimed subject is a method of modification of surfaces used as substrates for desorption-ionization mass spectrometry and immuno-chemical assays. The method is based on electronebulization (electrospraying) of protein solution, depending on the intended application either enzymes, lectins, or antibodies. The formed charged electrospray is dried in real time by its passing through an evaporation compartment and the resulting beam of desolvated ions impacts onto the surface and binds to it firmly. Such modified surface can be then used for a selective interaction with an affinity partner of the deposited protein, its preconcentration or enzymatic modification followed by an analysis by means of desorption-ionization mass spectrometry or immunochemical assays.
专利内容由知识产权出版社提供
专利名称：Method of Surface Modification by Proteins for Analyte Preconcentration for Desorption-Ionization Mass Spectrometry Techniques and for Immunochemical Assays
申请人：Petr Novak,Michael Volny,Petr Pompach,Viktor Ruzicka
地址：Dolni Brezany CZ,Praha 4 CZ,Mratin CZ,Rymarov CZ
国籍：CZ,CZ,CZ,CZ
更多信息请下载全文后查看