GM 1963 - C1 Rev. Sept 25 2001-YYY
Gem 操作指南中文
Nipro 产品数据册：单用途翼型肾脏细胞注射器说明书
H O S P I T A L P R O D U C T WING CATHSTERILE, SINGLE-USE WINGED CATHETER WITH INJECTION PORT FOR IV INFUSIONPRODUCT DATA SHEET2PRODUCT COMPLIANCE• CE-marked, Class IIa Medical Devices, Rule 7, MDD 93/42/EEC, UMDNS 10727• Complies with the following norms, directives, and regulations: ―ISO 594-1: 1986 ―EN 980:2008 ―EN 1041:2008 ―EN 1707:1996 ―ISO 7864:2016―ISO 9626:2016 ―EN ISO 10555-1:2013 ―EN ISO 10993-1/4/5/7/11 ―ISO 10993-10:2010 ―EN ISO 11135-1:2014―EN ISO 11607-1:2009 /AMD 2014 ―EN ISO 13485:2016 ―EN ISO 14971: 2012• Labels contain 12 languagesMANUFACTURING DETAILSLegal manufacturer: Nipro Corporation Country of origin: ThailandSTERILIZATION AND SHELF LIFESterilizationSingle-use only Indicator stickerShelf lifeEtO (Ethylene oxide)Each outer box (or shipping carton) contains a chemical indicator that indicates sterility. This indicator comes in the form of a [blue] sticker that turns[red] when sterilized.5 yearsWING CATHSTERILE, SINGLE-USE WINGED CATHETER WITH INJECTION PORT FOR IV INFUSION• Ultra sharp three-beveled needle to minimize discomfort • Radiopaque• Injection port with universal luer fitting • Flexible wings for easy grip• Color-coded wings and hub to indicate gauge• Siliconization of needle minimizes penetration and gliding force• T ransparent ETFE catheter tube for quick visualization of blood in the flashback chamber to facilitate insertion • Flashback in hub means the needle is correctly in the vein • Flashback in catheter means the catheter is correctly in the vein • Latex-free, DEHP-free, PVC-free • Available in 16-18-20-22-24 G• IV infusion duration: hours to several days • For use by healthcare professionals onlyClass IIa Medical DevicesRule 7MDD 93/42/EEC UMDNS 1072701233MATERIALS USEDDEHP-free Latex-free PVC-free PRODUCT RANGE OVERVIEWPACKAGING DETAILSLanguages12 languages on inner and outer box:English (EN), French (FR), Dutch (NL), German (DE), Spanish (ES), Italian (IT), Portuguese (PT), Greek (EL), Swedish (SV), Danish (DA), Norwegian (NO), and Finnish (FI)GlueMaterial: Acrylic resinNeedle (cannula)Material:Stainless steel SUS-304Needle hub Material:Polycarbonate (PC)Catheter hubMaterial: Polypropylene (PP)Port capMaterial: Polyethylene (PE)Catheter tubeMaterial: Ethyltetrafluoroethylene (ETFE)Cutting angleThree-beveledLuer capMaterial: Polypropylene (PP)Cap connectorMaterial: Polypropylene (PP)Caulking pinMaterial: Stainless steel SUS-304One-way valveMaterial: Silicone tubeLubricantMaterial: Silicone PolydimethylsiloxaneMembrane filterMaterial: Random micro glass filterVent fittingMaterial: Polypropylene (PP)Catheter wingMaterial: Polypropylene (PP)Nipro Medical Europe : European Headquarters, Blokhuisstraat 42, 2800 Mechelen, Belgium T: +32 (0)15 263 500 | F: +32 (0)15 263 510 |***********************| D a t a s h e e t - W i n g _C a t h - T H A - 27.N o v 2019Label detailsTransport conditionsClosed and dryStorage conditions Open the packaging only immediately before use to guarantee sterility.。
QPCR及QRT-PCR系列产品
Invitrogen的ICFC系列产品促销1．QPCR及QRT-PCR系列产品Invitrogen公司专门为中国客户提供的定量PCR试剂盒，结合了 UDG 防止残余污染技术和SYBR® Green I 荧光染料（存在于SYBR® Green I荧光定量PCR试剂盒中），在美国接受了严格的质量监控，可提供极高灵敏度的目的序列定量检测，线性剂量低，反应浓度范围很大。
qPCR Supermix-- 即用型反应剂，专为高特异性、实时定量DNA扩增设计UDG-- 防止携带污染物，减少克隆片段假阳性结果ROX参考染料-- 适用ABI仪器的校正染料产品信息活动时间：即日起至2009年4月30日2．Gibco南美胎牛血清即日起凡优惠价¥1780购买Gibco胎牛血清500ml（目录号:C2027050）即可获赠送价值¥250现金抵用券。
您可以凭现金抵用券在英韦创津公司购买任何商品，此券有效期至2009年5月31日。
产品信息活动时间：即日起至2009年4月30日独特的采集方式：GIBCO采用无菌心脏穿刺的方式采血原装直送，避免污染：原产地采集、加工、检测、包装。
完善的质控：采集、处理、检测、运输等环节都有文件和证书。
3．Invitrogen TA Cloning克隆产品专门用于克隆Taq聚合酶扩增的PCR产物。
采用pCR载体，能产生80%以上的重组产物，90%以上重组产物都包含插入片段。
产品信息活动时间：即日起至2009年5月31日附：pCR载体优点及图谱：3’-T突出端可直接连接Taq扩增的PCR产物可选择T7或T7和Sp6启动子进行体外RNA转录和测序侧向EcoRⅠ位点的通用多接头位点方便了插入片段的切离可以选择卡那霉素或氨苄青霉素进行筛选非常简便的蓝/白克隆筛选具有M13正向和反向引物位点，方便测序4．GIBCO液体培养基系列产品创立近50年的历史，品质优秀，产品种类丰富；为了中国用户利益，特建立国内生产线；所有产品，从原材料到生产全部按照GIBCO质量标准进行，每批均送抵美国公司总部质检合格后，才在国内销售。
尼罗红荧光染色溶液产品说明书(中文版)【模板】
尼罗红荧光染色溶液产品说明书（中文版）主要用途尼罗红荧光染色溶液是一种旨在通过与脂类物质的结合并发出荧光检测信号，快速、敏感、可靠地活体定量测定细胞内脂类成分的常用荧光染料。
适用于各种细胞包括细菌细胞，也用于蛋白质电泳染色。
产品严格无菌，即到即用，性能稳定，着色清晰灵敏。
技术背景尼罗红染色剂（9-diethylamino-5H-benzo[alpha]-phenoxazine-5-one；Nile red）是一种亲脂性的恶嗪类荧光染料。
与脂类物质包括腊酯（wax ester）和三酰甘油（triacylglycerol）以及各种脂肪酸结合后，在激发波长543nm的激发下，显示强烈桔红色荧光（散发波长598nm）。
同时在紫外光的照射下显示红色。
产品内容染色液（Reagent A）（0.5毫克/毫升）毫升产品说明书1份保存方式保存染色液（Reagent A）在－20℃冰箱里，避免光照，有效保证6月用户自备HANK平衡盐缓冲溶液（12028）或PBS缓冲溶液（12033）：用于清理细胞胰蛋白酶乙二胺四乙酸混合液（12024）：用于细胞脱离完全细胞培养液（12052）：用于细胞处理所需的培养基15毫升锥形离心管：用于细胞收集的存放微型台式离心机：用于细胞沉淀收集台式离心机：用于细胞沉淀收集1.5毫升离心管：用于细胞检测操作的容器2毫升离心管：用于染色工作液配制的容器37℃培养箱：用于孵育反应物比色皿：用于荧光定量分析荧光显微镜：用于观察荧光细胞荧光分光光度仪：用于定量检测荧光细胞和脂类产量方法一、间接法实验开始前，将试剂盒里的染色液（Reagent A）冻融，置入冰槽里。
然后进行下列操作。
1．开启荧光显微镜或荧光分光光度仪2．小心抽掉25cm2细胞培养瓶里的培养液3．加入3毫升用户自备的HANK平衡盐缓冲溶液或PBS缓冲溶液到细胞培养瓶，覆盖培养瓶表面4．小心抽掉清洗液5．加入1毫升用户自备的胰蛋白酶乙二胺四乙酸混合液，铺满整个培养平面6．置入37℃培养箱1分种7．振动培养瓶，使细胞脱落8．加入5毫升用户自备的完全细胞培养液9．移入15毫升锥形离心管10．放进台式离心机离心10分钟，速度为300g11．小心抽去上清液12．加入1毫升用户自备的HANK平衡盐缓冲溶液或PBS缓冲溶液，充分混匀13．移入到新的1.5毫升离心管14．加入xx微升染色液（Reagent A）到离心管15．用手指轻轻弹动离心管，使其充分混匀16．在37℃培养箱孵育10分钟，避免光照17．放进微型台式离心机离心30秒，速度为16000g（或13000RPM，例如eppendorf 5415）18．小心抽去上清液19．加入1毫升用户自备的HANK平衡盐缓冲溶液或PBS缓冲溶液，充分混匀20．（选择步骤）放进微型台式离心机离心30秒，速度为16000g（或13000RPM，例如eppendorf 5415）21．（选择步骤）小心抽去上清液22．（选择步骤）加入1毫升用户自备的HANK平衡盐缓冲溶液或PBS缓冲溶液，充分混匀23．检测方法A）荧光显微镜定性分析1）移出10微升离心管中的混匀物到载玻片上2）放上盖玻片或封片3）在荧光显微镜下观察荧光细胞：激发波长543nm，散发波长598nm――显示强烈桔红色荧光细胞的为脂类丰富的阳性细胞B）荧光分光光度计定量分析1）移取1毫升细胞悬液到1毫升比色皿2）放进荧光分光光度计测读：激发波长543nm，散发波长598nm――确定活体细胞脂类产量方法二：直接法实验开始前，将试剂盒里的染色液（Reagent A）冻融，然后移出2毫升用户自备的HANK平衡盐缓冲溶液或PBS缓冲溶液到2毫升离心管，加入xx微升染色液（Reagent A），混匀后，置入冰槽里，标记为染色工作液。
TYCO电子通用电源板电源RT1数据手册说明书
F0272-A V REG.-Nr. 6106, Z E214025Technical data of approved types on requestContact data RT.3T RTS3LContact configuration 1 NOContact set pre-make contact single contactType of interruption micro disconnectionRated current16ARated voltage / max.switching voltage AC 250/400VACLimiting continuous current 16AMaximum breaking capacity AC 4000VALimiting making capacitymax 20ms (incandescent lamps)165A120Amax 200μs (fluorescent lamps)800A-Contact material W (pre-make cont.)+AgSnO2AgSnO2Mechanical endurance DC> 5x106cycles> 10x106cyclesbistable> 3x106cycles> 5x106cyclestab manually operated> 103cycles-Rated frequency of operation with / without load 6 / 60 min-1Contact ratingsType Load CyclesRTS3T3000W, 230VAC, DF 8,3%, 5min-1, incandescent lamp typ. 12x103RT*3T16A, 250VAC, capacitive load 140μF, 7,5min-1, EN60669-1> 20x103RT*3T TV5, UL508, 40°C25x103RTS3L16A, 250VAC, 85°C> 100x103RTS3L 1.5 hp, 240 VACRTS3L TV8, UL508, 40°C25x103RTS3L10/100A/250VAC, simulated lamp load, acc. to IEC61810-220x103Coil dataCoil data,monostable coil Array Rated coil voltage range 5...110VDCCoil power typ 400 mWOperative range2Coil insulation system according UL1446 class FCoil versions,monostable DC-coilCoil Rated Operate Release Coil Rated coilcode voltage voltage voltage resistance powerVDC VDC VDC c mW0055 3.50.562+10%4030066 4.20.690+10%400012128.4 1.2360+10%4000242416.8 2.41440+10%4000484833.6 4.85520+10%4170606042.0 6.08570+12%42011011077.011.028800+12%420All figures are given for coil without preenergization, at ambient temperature +23°COther coil voltages on requestCoil data,bistable coils 1 coil 2 coilsRated coil voltage range 3...24VDCCoil powertyp 400mW typ 600mWOperative range2Limiting voltage, % of rated coil voltage 120%150%Minimum energization duration 30 msMaximum energization duration1 min at < 10% DFCoil insulation system according UL1446class FCoil versions,bistable 1 coil Coil Rated Operate Reset Coil Rated coil code voltage voltage voltage resistance powerVDC VDC VDC c mWA033 2.1 2.121+10%429A12128.48.4360+10%400A242416.816.81440+10%400Coil versions,bistable 2 coils F033 2.1 2.115+10%600F12128.48.4240+10%600F242416.816.8886+10%650All figures are given for coil without preenergization, at ambient temperature +23°C Other coil voltages on requestCoils - operation Version1 coil2 coilsCoil terminals A1A2A1A3A2 Pull-in +-+-Reset-+-+Contact position not defined at deliveryInsulationDielectric strength coil-contact circuit4000V rms open contact circuit1250V rms Clearance /creepage coil-contact circuit W 10 / 10mmMaterial group of insulation parts W IIIa Tracking index of relay base PTI 250 V Insulation to IEC 60664-1Type of insulation coil-contact circuitreinforced open contact circuitfunctional Rated insulation voltage 250 VPollution degree32Rated voltage system 240V400VOvervoltage categoryIIIOther data RT.3T RTS3LRoHS - Directive 2002/95/EC compliant Flammability class according to UL94V-0Ambient temperature range monostable -40...70°C -40...85°Cbistable: 1 coil -10...70°C -10...85°C bistable: 2 coils -40...70°C -40...85°CVibration resistance (function) monostable 10g 20g Shock resistance (destruction) 100g Category of protection RTII - flux proof Mounting pcb or on socket*)Mounting distance 0mm Resistance to soldering heat 270 °C / 10 s Relay weight with / without test tab 16 / 14g -/14g Packaging unit with / without test tab 100 / 500pcs -/500pcs *)RTT3T or bistable 2 coil version, pcb mounting only; see AccessoriesAccessories RTS3.For details see datasheetAccessories Power Relay RTTerminal assignmentBottom view on solder pinsS0163-CSa)a)Indicated contact position during or after coil energization with reset voltage.b)for 2 coil version onlymonostable versionbistable versionS0163-BFDimensions / PCB layout 16A, pinning 5mm*) With the recommended PCB hole sizes a grid pattern from 2.5mm to 2.54mm can be used.version without test tabversion with test tabS0491-Bb)for 2 coil version onlyS0272-BC。
迈勒泰尼生物技术说明书-MACS
.06Miltenyi Biotec B.V. & Co. KGpage 1/4Contents1. Description 1.1 Principle of the MACS® Separation 1.2 Background information 1.3 Applications1.4 Reagent and instrument requirements2. Protocol2.1 Sample preparation 2.2 Magnetic labeling 2.3 Magnetic separation2.4 C ell separation with the autoMACS® Pro Separator3. Example of a separation using CD45 (TIL) MicroBeadsWarningsReagents contain sodium azide. Under acidic conditions sodium azide yields hydrazoic acid, which is extremely toxic. Azide compounds should be diluted with running water before discarding. These precautions are recommended to avoid deposits in plumbing where explosive conditions may develop.1. DescriptionThis product is for research use ponents1 mL CD45 (TIL) MicroBeads, mouse:MicroBeads conjugated to monoclonal anti-mouse CD45 antibodies (isotype: rat IgG2b).CapacityFor 10⁹ total cells, up to 100 separations.Product format CD45 (TIL) MicroBeads are supplied in buffercontaining stabilizer and 0.05% sodium azide.StorageStore protected from light at 2−8 °C. Do not freeze. The expiration date is indicated on the vial label.1.1 Principle of the MACS® SeparationFirst, the CD45+cells are magnetically labeled with CD45 (TIL) MicroBeads. Then, the cell suspension is loaded onto a MACS® Column, which is placed in the magnetic field of a MACS Separator. The magnetically labeled CD45+ cells are retained within the column. The unlabeled cells run through; this cell fraction is thus depleted of CD45+ cells. After removing the column from the magnetic field, the magnetically retained CD45+ cells can be eluted as the positively selected cell fraction. To increase the purity, the positively selected cell fraction containing the CD45+ cells must be separated over a second column.1.2 Background informationCD45 (TIL) MicroBeads, mouse have been developed for theisolation of tumor-infiltrating leukocytes (TILs) from single-cell suspensions of solid mouse tumors. The CD45 antigen is expressed on all cells of hematopoietic origin except erythrocytes and platelets.1.3 Applications●Positive selection of CD45+ leukocytes from solid mouse tumors, e.g., B16F10, 4T1, or CT26.WT.1.4 Reagent and instrument requirements●Buffer: Prepare a solution containing phosphate-buffered saline (PBS), pH 7.2, 0.5% bovine serum albumin (BSA), and 2 mM EDTA by diluting MACS® BSA Stock Solution (# 130-091-376) 1:20 with autoMACS® Rinsing Solution (# 130-091-222). Keep buffer cold (2−8 °C). Degas buffer before use, as air bubbles could block the column. Always use freshly prepared buffer. ▲ Note: EDTA can be replaced by other supplements such as anticoagulant citrate dextrose formula-A (ACD-A) or citrate phosphate dextrose (CPD). BSA can be replaced by other proteins such as mouse serum albumin, mouse serum, or fetal bovine serum (FBS). Buffers or media containing Ca2+ or Mg2+ are not recommended for use.●MACS Columns and MACS Separators: CD45+ cells can be enriched by using MS or LS Columns. Positive selection can also be performed by using the autoMACS Pro or the MultiMACS™ Cell24 Separator.Positive selection MS 10⁷ 2 ×10⁷MiniMACS, OctoMACS, VarioMACS, SuperMACS II LS4 ×10⁷4 ×10⁷5 ×10⁷5 ×10⁷MidiMACS, QuadroMACS, VarioMACS, SuperMACS IIMultiMACS Cell24 Separator PlusautoMACS5 ×10⁷10⁸autoMACS ProMulti-24 Column Block (per column)2 ×10⁷2.5 ×10⁷MultiMACS Cell24 Separator Plus ▲Note: Column adapters are required to insert certain columns into the VarioMACS™ or SuperMACS™ II Separators. For details refer to the respective MACS Separator data sheet. ▲Note: If separating with LS Columns and the MultiMACS Cell24 Separator Plus use the Single-Column Adapter. Refer to the user manual for details.●Tumor Dissociation Kit, mouse (# 130-096-730) for the generation of single-cell suspension from tumor tissues.CD45 (TIL) MicroBeadsmouseOrder no. 130-110-618●gentleMACS™ Dissociator (# 130-093-235), gentleMACS OctoDissociator (# 130-095-937), or gentleMACS Octo Dissociatorwith Heaters (# 130-096-427)●gentleMACS C Tubes (# 130-093-237, # 130-096-334)●(Optional) Fluorochrome-conjugated REA (REAfinity™antibodies: recombinantly engineered, lacking Fcγ-bindingsite) CD45 antibodies for flow cytometric analysis, e.g., CD45-VioBlue®. For more information about antibodies refer to/antibodies.▲Note: Due to expression of Fcγ receptors on tumor-infiltrating leukocytesREA antibodies are recommended.●(Optional) Propidium Iodide Solution (# 130-093-233), DAPIStaining Solution (# 130-111-570), 7-AAD Staining Solution(# 130-111-568), or Viobility™ Fixable Dyes (# 130-109-812,# 130-109-814, # 130-109-816) for flow cytometric exclusion ofdead cells.●(Optional) Dead Cell Removal Kit (# 130-090-101) for thedepletion of dead cells.●(Optional) Pre-Separation Filters (30 µm) (# 130-041-407) toremove cell clumps.●(Optional) MACS SmartStrainers (30 µm) (# 130-098-458) toremove cell clumps.2. Protocol2.1 Sample preparationFor preparation of a single-cell suspension from solid mouse tumors use the Tumor Dissociation Kit, mouse (# 130-096-730) in combination with the gentleMACS™ Dissociators.For details refer to /protocols.▲ Dead cells may bind non-specifically to MACS® MicroBeads. To remove dead cells, we recommend using the Dead Cell Removal Kit (# 130-090-101).2.2 Magnetic labeling▲ Cells can be labeled with MACS MicroBeads using the autolabeling function of the autoMACS® Pro Separator. For more information refer to section 2.4.▲ Work fast, keep cells cold, and use pre-cooled solutions. This will prevent capping of antibodies on the cell surface and non-specific cell labeling.▲ Volumes for magnetic labeling given below are for up to 10⁷ total cells. When working with fewer than 10⁷ cells, use the same volumes as indicated. When working with higher cell numbers, scale up all reagent volumes and total volumes accordingly (e.g. for 2×10⁷ total cells, use twice the volume of all indicated reagent volumes and total volumes).▲ For optimal performance it is important to obtain a single-cell suspension before magnetic labeling. Pass cells through 30 µm nylon mesh (MACS SmartStrainers (30 µm), # 130-098-458). Moisten filter with buffer before use.▲ The recommended incubation temperature is 2–8 °C. Higher temperatures and/or longer incubation times may lead to non-specific cell labeling. Working on ice may require increased incubation times.1. Determine cell number.2. Centrifuge cell suspension at 300×g for 5 minutes. Aspiratesupernatant completely.3. Resuspend cell pellet in 90 µL of buffer per 10⁷ total cells.▲ Note: Always use freshly prepared buffer.4. Add 10 µL of CD45 (TIL) MicroBeads per 10⁷ total cells.5. Mix well and incubate for 15 minutes in the dark in therefrigerator (2−8 °C).6. (Optional) Add staining antibodies according tomanufacturer’s recommendations.7. Add buffer to a final volume of 500 μL for up to 5×10⁷ cells.▲Note: If more cells were used, split the sample onto multiple columns duringmagnetic separation.▲Note: For higher cell numbers, scale up buffer volume accordingly.8.Proceed to magnetic separation (2.3).2.3 Magnetic separation▲ Choose an appropriate MACS Column and MACS Separator according to the number of total cells and the number of CD45+ cells. For details refer to the table in section 1.4.▲ Note: MS Columns are recommended for highest purity of CD45+ cells. LSColumns are recommended for highest recovery of CD45+ cells.▲ For optimal performance it is important to obtain a single-cell suspension before magnetic separation. Pass cells through 30 µm nylon mesh (Pre-Separation Filters (30 µm), # 130-041-407) to remove cell clumps which may clog the column. Moisten filter with buffer before use.▲Always wait until the column reservoir is empty before proceeding to the next step.Magnetic separation with MS or LS Columns1. Place column in the magnetic field of a suitable MACSSeparator. For details refer to the respective MACS Columndata sheet.2. Prepare column by rinsing with the appropriate amount ofbuffer:MS: 500 µL LS: 3 mL3. Apply cell suspension onto the column. Collect flow-throughcontaining unlabeled cells.4. Wash column with the appropriate amount of buffer. Collectunlabeled cells that pass through and combine with theflow-through from step 3.MS: 3×500 µL LS: 2×1 mL▲ Note:Perform washing steps by adding buffer aliquots as soon as the columnreservoir is empty.5. Remove column from the separator and place it on a suitablecollection tube.6. Pipette the appropriate amount of buffer onto the column.Immediately flush out the magnetically labeled cells by firmly pushing the plunger into the column.MS: 1 mL LS: 3 mL7. (Optional) To increase the purity of CD45+cells, the elutedfraction can be enriched over a second MS or LS Column.Repeat the magnetic separation procedure as described in steps 1 to 6 by using a new column.Magnetic separation with the MultiMACS™ Cell24 Separator Refer to the the MultiMACS™ Cell Separator user manual for instructions on how to use the MultiMACS Cell24 Separator.2.4 Cell separation with the autoMACS® Pro Separator▲Refer to the user manual for instructions on how to use the autoMACS® Pro Separator.▲ All buffer temperatures should be ≥10 °C.▲ For appropriate resuspension volumes and cell concentrations, please visit /autolabeling.▲ Place tubes in the following Chill Rack positions:position A = sample, position B = negative fraction,position C = positive fraction.2.4.1 F ully automated cell labeling and separation1. Switch on the instrument for automatic initialization.2. Go to the Reagent menu and select Read Reagent. Scan the2D barcode of each reagent vial with the barcode scanner on the autoMACS® Pro Separator. Place the reagent into the appropriate position on the reagent rack.3. Place sample and collection tubes into the Chill Rack.4. G o to the Separation menu and select the reagent name foreach sample from the Labeling submenu (the correct labeling, separation, and wash protocols will be selected automatically).5. Enter sample volume into the Volume submenu. Press Enter.6. Select Run.2.4.2 M agnetic separation using manual labeling1. Label the sample as described in section2.2 Magnetic labeling.2. Prepare and prime the instrument.3. Apply tube containing the sample and provide tubes forcollecting the labeled and unlabeled cell fractions. Place sample and collection tubes into the Chill Rack.4. For a standard separation choose one the following programs:Positive selection:Posseld2for highest purityorPossels for highest recoveryCollect positive fraction in row C of the tube rack.3. Example of a separation usingCD45 (TIL) MicroBeadsA tumor induced by the B16F10 cell line was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, mouse. CD45+ TILs were isolated from the single-cell suspension using CD45 (TIL) MicroBeads, an MS Column, and a MiniMACS™ Separator.Cells were fluorescently stained with CD45-PE and Labeling-Check-Reagent-VioBlue® and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.Before separation10³-1110¹10²10³10²10¹CD45-PELabelingCheckReagent-VioBlue-11CD45+ cells10³-1110¹10²10³10²10¹CD45-PELabelingCheckReagent-VioBlue-11Refer to for all data sheets and protocols. Miltenyi Biotec provides technical support worldwide. Visit /local to find your nearest Miltenyi Biotec contact.Legal noticesLimited product warrantyMiltenyi Biotec B.V. & Co. KG and/or its affiliate(s) warrant this product to be free from material defects in workmanship and materials and to conform substantially with Miltenyi Biotec’s published specifications for the product at the time of order, under normal use and conditions in accordance with its applicable documentation, for a period beginning on the date of delivery of the product by Miltenyi Biotec or its authorized distributor and ending on the expiration date of the product’s applicable shelf life stated on the product label, packaging or documentation (as applicable) or, in the absence thereof, ONE (1) YEAR from date of delivery (“Product Warranty”). Miltenyi Biotec’s Product Warranty is provided subject to the warranty terms as set forth in Miltenyi Biotec’s G eneral Terms and Conditions for the Sale of Products and Services available on Miltenyi Biotec’s website at , as in effect at the time of order (“Product Warranty”). Additional terms may apply. BY USE OF THIS PRODUCT, THE CUSTOMER AGREES TO BE BOUND BY THESE TERMS.THE CUSTOMER IS SOLELY RESPONSIBLE FOR DETERMINING IF A PRODUCT IS SUITABLE FOR CUSTOMER’S PARTICULAR PURPOSE AND APPLICATION METHODS.Technical informationThe technical information, data, protocols, and other statements provided by Miltenyi Biotec in this document are based on information, tests, or experience which Miltenyi Biotec believes to be reliable, but the accuracy or completeness of such information is not guaranteed. Such technical information and data are intended for persons with knowledge and technical skills sufficient to assess and apply their own informed judgment to the information. Miltenyi Biotec shall not be liable for any technical or editorial errors or omissions contained herein.All information and specifications are subject to change without prior notice. Please contact Miltenyi Biotec Technical Support or visit for the most up-to-date information on Miltenyi Biotec products.LicensesThis product and/or its use may be covered by one or more pending or issued patents and/or may have certain limitations. Certain uses may be excluded by separate terms and conditions. Please contact your local Miltenyi Biotec representative or visit Miltenyi Biotec’s website at for more information.The purchase of this product conveys to the customer the non-transferable right to use the purchased amount of the product in research conducted by the customer (whether the customer is an academic or for-profit entity). This product may not be further sold. Additional terms and conditions (including the terms of a Limited Use Label License) may apply.CUSTOMER’S USE OF THIS PRODUCT MAY REQUIRE ADDITIONAL LICENSES DEPENDING ON THE SPECIFIC APPLICATION. THE CUSTOMER IS SOLELY RESPONSIBLE FOR DETERMINING FOR ITSELF WHETHER IT HAS ALL APPROPRIATE LICENSES IN PLACE. Miltenyi Biotec provides no warranty that customer’s use of this product does not and will not infringe intellectual property rights owned by a third party. BY USE OF THIS PRODUCT, THE CUSTOMER AGREES TO BE BOUND BY THESE TERMS.TrademarksautoMACS, gentleMACS, MACS, MACSQuant, MidiMACS, the Miltenyi Biotec logo, MiniMACS, MultiMACS, OctoMACS, QuadroMACS, REAfinity, SuperMACS, VarioMACS, Viobility, and VioBlue are registered trademarks or trademarks of Miltenyi Biotec and/or its affiliates in various countries worldwide.Copyright © 2020 Miltenyi Biotec and/or its affiliates. All rights reserved.。
Invitrogen Neon
Neon™ Transfection SystemFor transfecting mammalian cells, including primary and stem cells, with high transfection efficiency. Catalog Numbers MPK5000, MPK1025, MPK1096, MPK10025, MPK10096Doc. Part No. 25-1056 Pub. No. MAN0001632 Rev.B.0WARNING! For safety and biohazard guidelines, see the “Safety” appendix in the Neon™ Transfection System User Guide (Pub.No. MAN0001557). Read the Safety Data Sheets (SDSs) and follow the handling instructions. Wear appropriate protective eyewear, clothing, and gloves.Note: This Quick Reference is intended as a benchtop reference for experienced users of the Neon™ Transfection System User Guide (Pub. No. MAN0001557). For detailed instructions, supplemental procedures, and troubleshooting, see the Neon™ Transfection System User Guide (Pub. No. MAN0001557).General guidelines•Prepare high-quality plasmid DNA at a concentration of 1 to 5 μg/μL in deionized water or TE buffer, or high quality RNAi duplex at a concentration of 100–250 μM in nuclease-free water.•Use an appropriate GFP (green fluorescent protein) construct or siRNA control to determine transfection efficiency. See the Neon™Transfection System User Guide (Pub. No. MAN0001557) for details.•Use Resuspension Buffer R for established adherent and suspension cells, as well as primary adherent cells. Use Resuspension Buffer T with high voltage protocols of 1900 V or more. If arcing occurs with Resuspension Buffer R, consider switching toResuspension Buffer T.•Based on your initial results, you may need to optimize the electroporation parameters for your experiment using an 18-well or pre-programmed 24-well optimization protocol.•Discard the Neon™ Tips after 2 usages and Neon™ Tubes after 10 usages as a biological hazard. Change the tube and buffer when switching to a different plasmid DNA/siRNA or cell type.•The volume of plasmid DNA or siRNA added to the tranfection reaction should not exceed 10% of the total transfection volume.•Visit for a library of electroporation protocols for a variety of commonly used cell types.Prepare cellsFor the appropriate volume of medium to use based on cell density, or plating volumes for other plate formats, see “Amount of reagents” on page 2.1.Cultivate the required number of cells (70% to 90%confluent on the day of transfection) by seeding a flaskcontaining fresh growth medium 1 to 2 days prior toeletroporation.2.On the day of the experiment, pre-warm aliquots of culturemedium containing serum, PBS (without Ca2+and Mg2+), and Trypsin/EDTA solution to 37°C.3.Rinse the cells with PBS (without Ca2+and Mg2+), thentrypsinize the cells with the Trypsin/EDTA solution.4.Take an aliquot of trypsinized cell suspension, then countcells to determine the cell density.5.Harvest the cells in growth medium containing serum.6.Transfer cells to a 1.5-mL microcentrifuge tube or a 15-mLconical tube, then centrifuge the cells at 100 - 400 × g for 5 minutes at room temperature.7.Wash cells with PBS (without Ca2+and Mg2+) bycentrifugation at 100 - 400 × g for 5 minutes at roomtemperature.8.Aspirate the PBS, then resupsend the cell pellet inResuspension Buffer R (or Resuspension Buffer T forprograms ≥ 1900 V) at a final density of 1.0 × 107 cells/mL for adherent cells or 2.0 × 107 cells/ml for suspension cells.Gently pipette the cells to obtain a single cell suspension.IMPORTANT! Avoid storing the cell suspension for more than 15 to 30 minutes at room temperature. This will reduce cell viability and transfection efficiency.9.Prepare 24-well plates by filling the wells with 500 μL ofculture medium containing serum and supplements, butwithout antibiotics. Pre-incubate plates in a 37°C, 5% CO2 humidified incubator.Amount of reagentsFor each electroporation sample, the amount of plasmid DNA/siRNA, cell number, and volume of plating medium per well are listed in the following table. Use Resuspension Buffer T for cell types that require high voltage protocols of 1900 V or more. For all other cell types, use Resuspension Buffer R.[1]Use Resuspension Buffer T for primary suspension blood cells.Using the Neon ™Transfection SystemFor details on setting up the Neon ™device and Neon ™PipetteStation, see the Neon ™Transfection System User Guide (Pub. No.MAN0001557).1.Select the appropriate protocol for your cell type. Use one ofthe following options:•Input the electroporation parameters in the Input window if you already have the electroporation parameters for your cell type.•Tap Database , then select the cell-specificelectroporation parameters that you have added for various cell types.•Tap Optimization to perform the optimization protocol for your cell type.2.Fill the Neon ™Tube with 3 mL of Electrolytic Buffer (useBuffer E for the 10 μL Neon ™Tip and Buffer E2 for the 100μL Neon ™Tip).Note: Make sure that the electrode on the side of the tube is completely immersed in buffer. 3.Insert the Neon ™ Tube into the Neon ™Pipette Station untilyou hear a click sound (Figure 1).Figure 1 Schematic of Neon ™ Tube and Neon ™ Pipette Station.4.Transfer the appropriate amount of plasmid DNA/siRNA intoa sterile, 1.5 mL microcentrifuge tube.5.Add cells to the tube containing plasmid DNA/siRNA, thengently mix. See “Amount of reagents” on page 2 for cell number, DNA/siRNA amount, and plating volumes to use.6.To insert a Neon ™Tip into the Neon ™Pipette, press thepush-button on the pipette to the second stop to open the clamp.7.Insert the top-head of the Neon ™Pipette into the Neon ™Tipuntil the clamp fully picks up the mount stem of the piston (Figure 2).Figure 2 Schematic of Neon ™ Pipette and Neon ™Tip.8.Gently release the push-button, continuing to apply adownward pressure on the pipette, ensuring that the tip is sealed onto the pipette without any gaps.9.Press the push-button on the Neon ™Pipette to the first stopand immerse the Neon ™Tip into the cell-DNA/siRNA mixture.Slowly release the push-button on the pipette to aspirate thecell-DNA/siRNA mixture into the Neon ™Tip (Figure 3).Figure 3 Schematic of Neon ™Tip.Note: Avoid air bubbles during pipetting as air bubbles cause arcing during electroporation leading to lowered or failed transfection. If you notice air bubbles in the tip,discard the sample, then carefully aspirate the fresh sample into the tip again without any air bubbles.10.Insert the Neon ™Pipette with the sample vertically into theNeon ™ Tube placed in the Neon ™Pipette Station until youhear a click sound (Figure 4).Figure 4 Schematic of Neon ™ Tube and Neon ™ Pipette Station.Note: Ensure that the metal head of the Neon ™pipette projection is inserted into the groove of the pipette station.11.Ensure that you have selected the appropriateelectroporation protocol, then press Start on the touchscreen.12.The Neon ™device automatically checks for the properinsertion of the Neon ™ Tube and Neon ™Pipette before delivering the electric pulse.13.After delivering the electric pulse, Complete is displayed onthe touchscreen to indicate that electroporation is complete.14.Slowly remove the Neon ™Pipette from the Neon ™PipetteStation. Immediately transfer the samples from the Neon ™Tip by pressing the push-button on the pipette to the first stop into the prepared culture plate containing prewarmed medium with serum and supplements but without antibiotics.Note: Discard the Neon ™ Tip into an appropriate biologicalhazardous waste container. To discard the Neon ™Tip, press the push-button to the second stop into an appropriate biological hazardous waste container.15.Repeat step 6 to step 14 for the remaining samples.Note: Be sure to change the Neon ™Tips after using it twiceand Neon ™ Tubes after 10 usages. Use a new Neon ™Tip andNeon ™Tube for each new plasmid DNA sample.16.Gently rock the plate to ensure even distribution of the cells.Incubate the plate at 37℃ in a humidified CO 2 incubator.17.If you are not using the Neon ™device, turn the power switchon the rear to OFF .18.Assay samples to determine the transfection efficiency(e.g., fluorescence microscopy or functional assay) or geneknockdown (for siRNA).19.Based on your initial results, you may need to optimizedthe electroporation parameters for your cell type. For more information, see the Neon™ Transfection System User Guide (Pub. No. MAN0001557).Life Technologies Corporation | 5781 Van Allen Way | Carlsbad, California 92008 USAFor descriptions of symbols on product labels or product documents, go to /symbols-definition.The information in this guide is subject to change without notice.DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these products, you accept the terms and conditions of all applicable Limited Use Label Licenses.©2021 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified./support | /askaquestion。
海洋解淀粉芽孢杆菌GM—1培养基及摇瓶发酵条件优化
接种 １ 环菌株 Ｇ Ｍ一 １ 于盛有
５ ０ ｍＬ种子 培 养 基 的 ２ ５ ０ ｍＬ三 角 瓶 中 ， ２ ８ ￣ Ｃ， １ ８ ０ ＃ 芽 孢杆 菌 是 自然 界 中广 泛存 在 的 非致 病 细菌 ， 对 人畜无 害 、 不 污染 环境 ， 芽孢杆 菌产生 的低分 子抗 生 素 以及 蛋 白或多肽类 化合 物抗 菌活性 物质对 许多 危 害严 重 的植 物 病 原 真 菌 都 表 现 出很 强 的抑 菌 活
性， 且 因其 内生 芽孢 ， 抗逆性 强 ， 繁殖速度 快 ， 有利 于 工 业化 生产 ¨ Ｊ ， 在农 牧业 中具有 良好 的开 发 应用 前
ｍｉ ｎ振 荡培养 ２ ４ ｈ ， 调整菌 液浓度 为 １ ０ ｃ ｆ ｕ ／ ｍ Ｌ， 作发
酵 用种 子液 。
１ ． ２ ． ２ 摇 瓶发 酵培养
摘
要
Ｇ Ｍ． １菌株 为分 离 自海 洋对 油 菜 菌核
菌具 有 强 烈 抑 制 作 用 的 解 淀 粉 芽 孢 菌 菌 株 Ｇ Ｍ一 １ ， 表 现 出 良好 的生 防 应 用 前 景 。采 用 单 因子 和 正交试 验设 计对该 菌 株 的发 酵 条 件进 行 优 化 ， 旨在 为该 菌株 生防制 剂 的开 发和应 用提供 理论基 础 。
０Ｄ６ ｏ ０ 。
景， 一些 菌株 已用 于植 物病 害 的生物 防治 ， 如 苏云金 芽孢杆 菌 （ Ｂ ａ ｃ ｉ ｌ ｌ ｕ ｓ ｔ ｈ ｕ ｒ ｉ ｎ ｇ ｉ ｅ ｎ ｓ ｉ ｓ ） Ｊ 、 枯 草芽孢 杆 菌
以 油 菜 菌 核 病 菌
（ Ｂ ａ ｃ ｉ ｌ ｌ ｕ ｓ ｓ ｕ ｂ ｔ ｉ ｌ ｉ ｓ ） ［ ４ ｊ 、 解 淀 粉 芽孢 杆 菌 （ Ｂ ａ ｃ ｉ ｌ ｌ ｕ ｓ ａ ｍ ｙ ｌ ｏ ｌ ｉ ｑ ｕ ｅ ｆ ａ ｃ ｉ ｅ ｎ ｓ ） 等。海洋具有寡营养的独特 环
维克森公司产品目录说明书
8Catalog 9EM-TK-190-1Pneumatic Division Richland, MichiganDimensionsF16-02-000Particulate Filter F16SpecificationsFlow Capacity* 1/4 63.0 SCFM (29.7 dm 3/s) 3/8 74.1 SCFM (34.9 dm 3/s)1/280.4 SCFM (37.9 dm 3/s)Operating Plastic Bowl 32° to 125°F (0° to 52°C) Temperature Metal Bowl 32° to 150°F (0° to 65.5°C)Maximum Supply Plastic Bowl 150 PSIG (10.3 bar) Pressure Metal Bowl 200 PSIG (13.8 bar)Port SizeNPT / BSPP-G1/4, 3/8, 1/2Standard Filtration Micron5Useful Retention** oz. (cm 3) 2.7 (81)Weightlb. (kg)1.8 (0.8)* Inlet pressure 150 PSIG (10.3 bar). Pressure drop 5 PSID (0.3 bar).** Useful retention refers to volume below the quiet zone baffle.“F” Series Filters, Type “A” 5 micron elements: All Wilkerson Type “A” 5 micron elements meet or exceed ISO Class 3 for maximum particle size and concentration of solid contaminants.Materials of ConstructionBaffle PolypropyleneBodyZincBowls Plastic Bowl PolycarbonateMetal Bowl Zinc DeflectorPolypropyleneElement Retainer Acetal Filter ElementPolyethylene Seals Plastic BowlNitrileMetal Bowl Fluorocarbon Sight GaugeMetal BowlPolycarbonateFeatures• Manual Drain• 5 Micron Rated Element• Quick-disconnect Bowl Guard with Integral Plastic Bowl and Safety LatchAuto DrainManual DrainCatalog 9EM-TK-190-19Pneumatic Division Richland, MichiganOrdering InformationReplacement Bowl KitsMetal Bowl –Automatic Drain ...................................................FRP-95-950 Manual Drain .......................................................FRP-95-178Sight Gauge, Manual Drain.................................GRP-95-133Plastic Bowl –Bowl Guard, Automatic Drain ..............................FRP-95-015 Bowl Guard, Manual Drain ..................................FRP-95-014 Manual Drain .......................................................FRP-95-017Replacement Element KitsType “A”, 5 Micron ...................................................FRP-95-160AccessoriesAutomatic Drain, Nitrile .........................................GRP-95-973L-Bracket .................................................................GPA-95-016Manual Drain ...........................................................FRP-95-610Sight Gauge Kit ......................................................GRP-95-079123450.10.20.31051520250AIR FLOW RATEP R E S S U R E D R O Pb a rdm 3/s1234500.10.20.3P R E S S U R E D R O Pb a rAIR FLOW RATEdm 3/s012345P R E S S U R E D R O P10203040AIR FLOW RATEdm 3/s0.10.2b a r00.3Particulate Filter F16(Revised 5-4-07)。
动物细胞组织细胞色素C释放凋亡检测试剂盒产品说明书(中
动物细胞/组织细胞色素C释放凋亡检测试剂盒产品说明书（中文版）主要用途动物细胞/组织细胞色素C释放凋亡检测试剂是一种旨在从动物细胞或活体组织中分离出线粒体和细胞浆组分，从而检测细胞色素C的转运，即由线粒体释放到细胞浆，来探测细胞凋亡的权威而经典的技术方法。
该技术经过精心研制、成功实验证明的。
适合于动物软组织（肝或脑组织）和硬组织（肌肉）以及培养细胞的线粒体和细胞浆组分的制备。
可以被用于后续蛋白质西方杂交等实验。
产品不含污染性蛋白水解酶和核酶，即到即用，性能稳定，分离到位，质量保证。
技术背景细胞色素C（CYTOCHROME C）在细胞凋亡中扮演着重要作用。
细胞色素C位于线粒体内外膜之间。
当细胞凋亡时，它从线粒体内释放到细胞浆里，引起一系列的信号通道的下游反应。
产品内容清理液（Reagent A）毫升裂解液（Reagent B）毫升净化液（Reagent C）毫升强化液（Reagent D）毫升保存液（Reagent E）毫升活性液（Reagent F）微升溶解液（Reagent G）毫升上样液（Reagent H）微升产品说明书1份保存方式保存净化液（Reagent C）和活性液（Reagent F）在-20℃冰箱里，避免反复冻融；其余的保存在4℃冰箱里；有效保证6月用户自备HANK平衡盐缓冲溶液（GMS12028）或PBS缓冲溶液（GMS12033）：用于清理细胞硬组织处理液（Reagent I）：用于促进硬组织表面溶解胰蛋白酶乙二胺四乙酸混合液（GMS12024）：用于细胞脱离完全细胞培养液（GMS12052）：用于细胞培养所需的培养基15毫升锥形离心管：用于样品操作的容器50毫升锥形离心管：用于样品操作的容器1.5毫升离心管：用于样品操作的容器4℃（微型）台式离心机：用于样品操作DOUNCE匀浆器：用于裂解组织细胞抗细胞色素C抗体：用于蛋白质西方杂交实验步骤一、动物软组织线粒体分离（组织匀浆法）实验开始前，将试剂盒里的活性液（Reagent F）冻融，然后移出xx微升活性液（Reagent F）和xx毫升净化液（Reagent C）到xx毫升的裂解液（Reagent B）里，混匀后，放进冰槽里，标记为裂解工作液。
碧云天生物技术产品说明书 - pCMV-mCherry-p62
碧云天生物技术/Beyotime Biotechnology 订货热线：400-168-3301或800-8283301 订货e-mail ：******************技术咨询：*****************网址：碧云天网站 微信公众号pCMV-mCherry-p62产品编号 产品名称 包装 D2818-1μg pCMV-mCherry-p62 1μg D2818-100μgpCMV-mCherry-p62100μg产品简介：pCMV-mCherry-p62是碧云天研发的在哺乳动物细胞中表达红色mCherry 标签的人源p62融合蛋白的质粒。
该质粒含有CMV 启动子，为卡那霉素抗性，转染后能够在靶细胞中高效表达带有红色荧光蛋白mCherry 标签的p62融合蛋白，呈现明亮的红色荧光，可以用于细胞自噬(autophagy)的研究。
本质粒转染细胞后，可以使用G418筛选稳定表达融合蛋白的细胞株。
p62也称sequestosome I (SQSTM1)，在多细胞生物(不包括植物和真菌)中高度保守，主要分布于细胞质，也可以定位于细胞核、自噬体(autophagosome)和溶酶体(lysosome)中。
p62是一种应激诱导的蛋白，可以作为信号枢纽(signaling hub)在氨基酸感应(amino acid sensing)和氧化应激等许多细胞事件中发挥重要作用，并且还可以作为PKC 、ERK1、mTORC1、NF-кB 和caspase-8等的支架蛋白(scaffold protein)而参与相应的信号转导。
p62全长440个氨基酸，包含一个PB1(Phox1/Bem1p)结构域，一个锌指结构域(ZZ)，两个核定位信号(NLS1和NLS2)，一个TB (TRAF6 binding)结构域，一个LIR 结构域(LC3-interacting region)，一个KIR 结构域(Keap1-interacting region)，和一个C 端的UBA(Ubiquitin-associated)结构域。
HUMAN EMBRYONIC KIDNEY (HEK) 293 SUBCLONE CELL ST
专利名称：HUMAN EMBRYONIC KIDNEY (HEK) 293 SUBCLONE CELL STRAIN发明人：ZHANG, Xiaozhi,PENG, Zhaohui申请号：CN2004000457申请日：20040509公开号：WO04/111239P1公开日：20041223专利内容由知识产权出版社提供摘要：The present invention disclosed a HEK 293 subclone cell strain for producing recombinant adenovirus preparation, the said cell strain, which comprises human glyceraldehydes-3-phosphate dehydrogenase (GAPD), is made of ATCC 293 cell via subcloning method. The producing method includes the following steps: a. proliferating the ATCC 293 cell (P32) to P33, and then using FACS flow cytometer to subclone the P33 suspension; b. using two 96-well plates, wherein placing a cell in 0.2ml medium of every cell, and culturing at 3638C, 3-6 % C02 for 6- 10days; c. selecting the clones with the cell uniform, growth well and not less than 60% confluent culture; d. repeating the above steps 4times, and finally selecting a cell strain(P37) of subclone, continuing to amplify and obtain the proto-cell library(P38), and freezing under - 80'C; e. preparing the parental cell library and working cell library based on proto-cell library. The present invention makes it having the advantages of increasing the function of the cells adherence growth and inducing the multiplication time of cell growth substantially.申请人：ZHANG, Xiaozhi,PENG, Zhaohui地址：CN,CN国籍：CN,CN代理机构：JEEKAI & PARTNERS 更多信息请下载全文后查看。
SequencingPrimers
M13 Reverse M13/pUC Forward M13/pUC Reverse MBP-F mCherry-F mCherry-R MT Forward MMLV-F Moloney murine leukemia virus LTR (MoMuLV), forward primer mPGK-F MSCV MSCV-rev MT1-F mU6-F Myc Neo-R NOS-F Nmt1-F S. pombe nmt1 pБайду номын сангаасomoter, forward primer OpIE2 Forward pACYC-F pAd-CMV pBABE 3' pBABE 5' pBAD Forward pBAD Reverse pBluescriptKS pBluescriptSK pBMN 5' MMLV sequence, for inserts in pBMN retroviral vector pBR322ori-F pBRforBam pBRforEco pBRrevBam pCAG-F pCasper-F pcDL-F
SequencingPrimers
Commonly Used Primers
Full Primer List GCAAATGGCATTCTGACATCC (Invitrogen) 3'AOX1 5'AOX1 35S promoter AC5 Alpha-factor Amp-R AUG1 Forward BGH Reverse Bglob-intron-F Bglob-intron-R Rabbit beta-globin intron, reverse primer Bglob-pA-R CAT-R CMV Forward CRE-R CYC1 DsRed1-C DsRed1-N
巨噬细胞mtorc1 精氨酸代谢
巨噬细胞mtorc1 精氨酸代谢（中英文实用版）Title: Macrophage mTORC1 and Arginine Metabolism英文：The mammalian target of rapamycin complex 1 (mTORC1) is a key regulator of cell growth, survival, and metabolism.In macrophages, mTORC1 plays a critical role in controlling inflammation and immune responses.One of the important functions of mTORC1 is to regulate the metabolism of arginine, an essential amino acid.Arginine metabolism is essential for macrophage function, and imbalances in arginine metabolism have been associated with various diseases, including cancer, diabetes, and cardiovascular diseases.中文：哺乳动物雷帕霉素靶蛋白复合体1（mTORC1）是细胞生长、生存和代谢的关键调节器。
在巨噬细胞中，mTORC1在控制炎症和免疫反应中发挥关键作用。
mTORC1的一个重要功能是调节精氨酸代谢，精氨酸是一种必需氨基酸。
精氨酸代谢对巨噬细胞功能至关重要，精氨酸代谢失衡与多种疾病有关，包括癌症、糖尿病和心血管疾病。
英文：The mTORC1 pathway is activated by nutrient availability, growth factors, and other signals.In the context of arginine metabolism, mTORC1 promotes the synthesis of arginine by regulating the expression ofarginine-related genes.This synthesis is important for macrophage proliferation and survival.Additionally, mTORC1 activates the translation of arginine-rich proteins, which are involved in various cellular processes, including translation, transcription, and cell signaling.中文：mTORC1途径通过调节营养素可用性、生长因子和其他信号来激活。
肠杆菌科细菌编码微量鉴定管使用中的注意事项
肠杆菌科细菌编码微量鉴定管使用中的注意事项于建华，闫文婧（解放军第医院检验科，山东淄博）关键词：肠杆菌；鉴定；编码中图分类号：肠杆菌科的细菌是造成各种感染的常见菌，对于它的鉴定，虽然可用全自动微生物鉴定仪，但大多数中、小医院不具备条件，而按传统方法鉴定必须进行一系列生化反应和血清学试验，不仅造成人力、材料的浪费，而且需要长时间等待结果甚至造成漏检。
采用肠杆菌科编码的方法，简便易行，结果及时可靠。
但该法在使用编码微量鉴定管过程中，发现有一些影响鉴定结果的因素，必须引起注意。
!接种生化鉴定管分离培养出的细菌，首先观察菌落特征。
氧化酶阴性、发酵型的革兰阴性杆菌可按肠杆菌科处理。
取单一菌落或纯的细菌培养物接种生化管，应每接种一管均需灭菌，但微量生化管数量多，耗时长，在实际工作中多采用一次取菌连续接种!管，为了避免生化管成分交叉感染造成假性结果，可合理配组减少影响。
如：（）赖氨酸、鸟氨酸、氨基酸；（）乳糖、卫茅醇、双糖铁；（）硫化氢、尿素；（）枸橼酸盐、蛋白胨水。
组间顺序可变，与单一管接种是一致的。
另外，在主要试验和辅助试验接种过程中，苯丙氨酸、葡萄糖酸盐管接种量要大，否则会造成假阴性；枸橼酸盐管接种量要小，避免将培养基成分带入而出现可疑反应；半固定接种应在中间垂直穿入近底部，但管径小，扩散生长并不明显，特别是动力弱的菌株，沿管壁穿刺较易观察，同时参考悬滴法或压片法。
氨基酸脱羧试验一定用灭菌液体石蜡!封口，否则会产生不稳定的胺而被氧化造成假阳性。
"结果判断肠杆菌科的细菌多属于快速生长菌，采用肠杆菌微量鉴定管时多数结果，!时观察结果。
如糖、醇类（迟缓分解除外）、苯丙氨酸、葡萄糖酸盐、靛基质、硫化氢、氨基酸脱羧试验等。
但也有特殊者，观察不妥，如：（）甲基红试验：克雷伯菌、肠杆菌属的常见菌，如后加试剂观察呈阳性（红），而后变为阴性（黄）；（）尿素酶试验，!呈阳性（红色），不变为阴性，当疑为变形杆菌属应分段观察，此菌属!可迅速分解尿素，具有鉴别意义。