沉默Sp1抑制大肠癌细胞SW480的端粒酶活性并促进细胞凋亡(英文)

沉默Sp1抑制大肠癌细胞SW480的端粒酶活性并促进细胞
凋亡(英文)
Liguo Zhao Yan Zhu Wantong Niu Meining Li Niuliang Cheng
【期刊名称】《中德临床肿瘤学杂志：英文版》
【年(卷),期】2011(10)4
【摘要】Objective:The aim of the study was to examine the effect of Sp1 on the expression of the human telomerase reverse transcriptase(hTERT) gene in human colorectal carcinoma SW480 cells.Methods:The Sp1 shRNA plasmid was transfected into colorectal carcinoma SW480 cells line by liposome mediation for transient expression.After Sp1 shRNA plasmid transfected SW480 cells,the exogenous Sp1 protein expression was determined by the method of Western blot.At same time,hTERT mRNA expression was detected by RT-PCR,telomerase activity was determined by the telomeric repeat amplification protocol(TRAP) assay,and the apoptotic rate of cells was also tested by flow cytometry.Results:The protein expressions of Sp1 gene could be reduce by transfecting of pGenesil-1-Sp1(+) recombinant plasmid into SW480 cells.The apoptotic rate was increased compared with pGenesil-1-Sp1(-)/SW480 and SW480(P <
0.05),which indicated that lowexpression of Sp1 gene could lead to low level of telomerase activity and induce apoptosis.Conclusion:Silencing Sp1 may suppress the activity of telomerase by inhabiting hTERT gene expression.
【总页数】5页(P220-224)
【关键词】人端粒酶逆转录酶;端粒酶活性;细胞凋亡;Sp1;细胞系;大肠
癌;hTERT;shRNA
【作者】Liguo Zhao Yan Zhu Wantong Niu Meining Li Niuliang Cheng 【作者单位】Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan 030001, China
【正文语种】中文
【中图分类】Q55;Q255
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