Temozolomide_NSC362856_CCRG81045_Autophagy_CAS号85622-93-1说明书_AbMole中国
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分子量194.15
溶解性（25°C）
DMSO 39 mg/mL
分子式C H N O Water 5 mg/mL
CAS号85622-93-1Ethanol <1 mg/mL
储存条件3年 -20°C 粉末状
生物活性
Temozolomide是一种DNA损伤诱导剂。
实验操作来自于公开的文献，仅供参考
细胞实验
细胞系A2058, A375, M238 and M249 cell lines
方法Colony formation assay Depending on the cell line (and plating efficiency), 200–1000 cells were seeded into each well of a 6-well plate and treated as described in detail previously. In the case of primary melanoma cells, 1000 cells were seeded and let grow for 24 days
in the presence or absence of drug treatment; because only small colonies formed, the stained colonies were counted under the
microscope.Survival of melanoma cells after drug treatment. Five different melanoma cell lines (as indicated), as well as a culture of
primary melanoma tissue cells (labeled ‘primary’) were exposed to increasing concentrations of TMZ (diamonds) or NEO212 (circles)
for 48 hours, and long-term survival was determined via colony formation assay (CFA).
浓度0, 25, 50, 75, 100µM
处理时间48 h
动物实验
动物模型A375 cells subcutaneous tumor growth model
配制45% glycerol, 45% ethanol, 10% DMSO
剂量50 mg/kg
给药处理subcutaneous injection
不同实验动物依据体表面积的等效剂量转换表（数据来源于FDA指南）
小鼠大鼠兔豚鼠仓鼠狗
重量 (kg)0.020.15 1.80.40.0810
体表面积 (m)0.0070.0250.150.050.020.5
K系数36128520
动物 A (mg/kg) = 动物 B (mg/kg) ×
动物 B的K系数
动物 A的K系数
例如，依据体表面积折算法，将白藜芦醇用于小鼠的剂量22.4 mg/kg 换算成大鼠的剂量，需要将22.4 mg/kg 乘以小鼠的K系数（3），再除以大鼠的K系数（6），得到白藜芦醇用于大鼠的等效剂量为11.2 mg/kg。
参考文献
Human monocytes undergo excessive apoptosis following temozolomide activating the ATM/ATR pathway while dendritic cells and macrophages are resistant. Bauer M, et al. PLoS One. 2012;7(6):e39956. PMID: 22768182.
Temozolomide 目录号M2129
化学数据
6662
2
m
m
m
m m
Inhibition of mitochondria- and endoplasmic reticulum stress-mediated autophagy augments temozolomide-induced apoptosis in glioma cells.
Lin CJ, et al. PLoS One. 2012;7(6):e38706. PMID: 22745676.
Preclinical antitumor activity of temozolomide in mice: efficacy against human brain tumor xenografts and synergism with 1,3-bis(2-chloroethyl)-1-nitrosourea. Plowman J, et al. Cancer Res. 1994 Jul 15;54(14):3793-9. PMID: 8033099.