槲皮素增强阿霉素抗乳腺癌活性及机制的研究

槲皮素增强阿霉素抗乳腺癌活性及机制的研究

张阳阳;赵冉;李美欣;李世正

【期刊名称】《辽宁医学院学报》

【年(卷),期】2016(037)006

【摘要】Objective To investigate the feasibility and mechanism of quercetin enhancing the adriamycin activity in breast canc-er. Method Effect of Que with different concentration on the growth of human breast cancer MCF-7 and MCF-7/ dox cells was ex-amined by MTT assay. Human breast cancer MCF-7 and MCF-7/ dox cells were treated with ADR alone or ADR with non-cytotoxic dose of Que. Then, proliferative capacity of the cells was measured by using MTT assay, intracellular ADR concentration and cell ap-optosis were analyzed by using flow cytometry, and P-gp expression was detected by Western b1ot assay. Result Que less than 0.

7μM had non-cytotoxic effect on the growth of human breast cancer MCF-7 and MCF-7/ dox cells. Compared with treatment with ADR alone, combined treatment of ADR and quercetin (0. 7μM) significantly inhibited breast cancer cell proliferation, increased the intra-cellular ADR concentration and cell apoptosis, and suppressed the expression of P-gp. Conclusion Que can enhance the sensitivity of adriamycin to breast cancer cells, mechanism of which may be related to the down-regulation of P-gp expression in the cells.%目的：探讨槲皮素( quercetin, Que)增强阿霉素抗乳腺癌活性的可行性及其相关机制。方法 MTT 法检测不同浓度 Que 对人乳腺癌

MCF-7和MCF-7/dox细胞生长的影响,单用阿霉素( adriamycin, ADR)和ADR 联合无毒剂量Que作用于人腺癌MCF-7和MCF-7/dox细胞, MTT法检测药物对细胞的增殖抑制作用,流式细胞法检测细胞内ADR的积累和细胞凋亡率；Western blot法检测P-糖蛋白( P glycoprotein, P-gp)的表达变化。结果不高于0.7μM Que对人腺癌MCF-7和MCF-7/dox细胞生长无影响。与单用ADR组相比较,0.7μM Que可提高ADR对乳腺癌细胞的增殖抑制率、增加乳腺癌细胞内ADR的积累和细胞凋亡率、下调P-gp的表达。结论 Que 能够增强阿霉素抗乳腺癌细胞化疗敏感性,其机制可能与下调该细胞P-gp表达有关。

【总页数】3页(P9-10,14)

【作者】张阳阳;赵冉;李美欣;李世正

【作者单位】锦州医科大学附属第一医院普外科，辽宁锦州121001;锦州医科大学附属第一医院普外科，辽宁锦州121001;锦州医科大学附属第一医院普外科，辽宁锦州121001;锦州医科大学附属第一医院普外科，辽宁锦州121001

【正文语种】中文

【中图分类】R285

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