雾化吸入L-精氨酸对哮喘小鼠气管急性炎症的影响及机制

雾化吸入L-精氨酸对哮喘小鼠气管急性炎症的影响及机制陈艳妮;邱琴;闫珊;刘美娟;高福生
【期刊名称】《山东医药》
【年(卷),期】2016(56)12
【摘要】Objective To observe the effect of aerosol inhalation of L-arginine on acute airway inflammation in mice model with asthma and to investigate the mechanism.Methods Thirty male BALB/c mice were randomly divided into three groups and 10 mice in each group:L-arginine group, model group and the control group.The L-arginine group and model group were sensitized by intraperitoneal ( i.p.) injections of 0.3 mL suspension of 100 μg OVA and 2 mg AL (OH)3, and the control group was injected with 0.3 mL PBS at the same time.Since the 21st day, these three groups were put into homemade glass atomization box.L-arginine group and model group were given 30 g/L OVA for aerosol inhalation and the control group with PBS for aerosol inhalation, once a day, thirty minutes one time and for 12 days.L-arginine group was given 174 mg/mL L-arginine for aerosol inhalation for 30 minutes before every sensitization.Twenty-four hours after the last sensitization, mice of the three groups were bled to death, and then we collected the left lung tissues and the bronchoalveolar lavage fluid.HE staining was used on the upper left lobe of lung tissue, and the pathological changes were observed.We counted the total number of leukocytes, esosinophils,
lymphocytes and macrophages.The levels of interler-kin-4(IL-4), NO and interferon-γ(IFN-γ) in BALF were measured by ELISA.Results In the model group, the airway epithelium fractured, fell off and had mucous edema with more folds under HE staining, and there was inflammatory cell in-filtration with a large number of esosinophils and lymphocytes around the bronchus.The inflammatory cell infiltration and mucous edema in the L-arginine group was less than that of the model pared with the control group, the leuko-cytes, esosinophils, lymphocytes and macrophages in BALF were increased in the L-arginine group and the model group, and the above indexes of the L-arginine group were less than those of the model group (all P＜0.05).Compared with the control group, the levels of IL-4 and IL-4/IFN-γwere increased, but the levels of IFN-γand NO were decreased in the L-arginine group and model group;compared with the model group, the levels of IL-4 and IL-4/IFN-
γdeclined, but IFN-γand NO elevated in the L-arginine group, and significant difference was found among these groups (all P＜0.05).Con-clusion The aerosol inhalation of L-arginine can inhibit the airway inflammation of asthmatic mice, and its mechanism may be related to inhibiting IL-4 expression, promoting IFN-γexpression and the production of NO.%目的观察雾化吸入L-精氨酸对支气管哮喘(简称哮喘)小鼠气管急性炎症的影响,探讨其作用机制.方法 30只雄性BALB/c小鼠随机分为三组,每组10只.L-精氨酸组、模型组分别于第1、8、15天腹腔注射含100μg卵清蛋白干粉剂(OVA)和2 mg氢氧化铝粉的混悬液0.3 mL致敏,对照组于相同时间腹腔注射PBS 0.3 mL.第21天起将三组分别放于自制玻璃雾化箱内,L-精氨酸组、模型组以30 g/L
OVA进行雾化吸入,对照组雾化吸入PBS,均1次/d、30 min/次、连续12 d.每次雾化激发前30 min,L-精氨酸组雾化吸入L-精氨酸174 mg/mL.末次雾化吸入24 h放血处死所有小鼠,留取左肺组织及右肺支气管肺泡灌洗液(BALF).将左肺上叶组织切片进行HE染色,观察支气管病理变化;取BALF沉渣,计数白细胞总数及嗜酸性粒细胞、淋巴细胞、巨噬细胞数量;采用ELISA法检测BALF中IL-4、IFN-γ及NO.结果 HE染色见模型组气道上皮断裂、脱落,黏膜水肿、皱褶增多;支气管周围有大量以嗜酸性粒细胞、淋巴细胞为主的炎性细胞浸润.L-精氨酸组气道周围炎细胞浸润及黏膜水肿均较模型组减轻.与对照组比较,模型组、L-精氨酸组BALF中白细胞总数及嗜酸性粒细胞、淋巴细胞、巨噬细胞数量均增多,L-精氨酸组上述细胞数量均较模型组减少,组间比较P均＜0.05.与对照组比较,模型组及L-精氨酸组BALF中IL-4水平、IL-4/IFN-γ明显升高,IFN-γ及NO降低;与模型组比较,L-精氨酸组BALF中IL-4、IL-4/IFN-γ降低,IFN-γ及NO升高;组间比较P均＜0.05.结论L-精氨酸雾化吸入对哮喘小鼠气管炎症有抑制作用,其作用机制可能与抑制IL-4表达、促进IFN-γ表达和NO产生有关.
【总页数】4页(P28-30,Ⅱ)
【作者】陈艳妮;邱琴;闫珊;刘美娟;高福生
【作者单位】潍坊医学院,山东潍坊261000;潍坊医学院,山东潍坊261000;潍坊医学院,山东潍坊261000;潍坊医学院,山东潍坊261000;潍坊医学院,山东潍坊261000
【正文语种】中文
【中图分类】R562.2
【相关文献】
1.雾化吸入L-精氨酸治疗高原肺水肿对血液流变性的影响 [J], 崔建华;张西洲;朱永安;王伟;马勇;张芳
2.L-精氨酸L-门冬氨酸盐对血栓形成的影响及其机制 [J], 王银叶;刘晓岩;王景燕;王超;李春波;毛伟;彭师奇
3.L-精氨酸雾化吸入治疗支气管哮喘急性发作 [J], 刘希文;陈志刚;王建国;赵兰江
4.布地奈德雾化吸入对支气管哮喘小鼠肺组织NF-κB及OSM表达的影响 [J], 高亚丰;祁军利;高雷
5.茶多酚对L-精氨酸诱导的小鼠急性胰腺炎的影响及机制分析 [J], 席俊彦;庾镇藩;杨莹;汤银娟;王建钧
因版权原因，仅展示原文概要，查看原文内容请购买。