止咳枇杷糖浆中齐墩果酸及熊果酸含量的高效液相色谱测定

止咳枇杷糖浆中齐墩果酸及熊果酸含量的高效液相色谱测定李建芳;黄劲通
【期刊名称】《中国医药指南》
【年(卷),期】2013(11)2
【摘要】Objective To establish the determination method of Oleanolic acid and Ursolic in Zhike Pipa Tangjiang by HPLC. Methods The separation was out on a Apollo C18 column , with mobile phase of acetonitrile-methanol-0.5%ammonium acetate(67∶12∶21). The flow rale was set at 1.0mL/min and the detection wavelength was at 210nm. Result The calibration curve of oleanolic acid was in good linear of 0.1006-1.5090μg, (r=0.9999), and the average recovery rate was 98.73%, RSD=1.05%. Ursolic showed good linear of 0.4028-6.0420μg, (r=0.9997), and the average recovery rate was 99.25%, RSD=1.54%. Conclusion The developed method is sensitive, accurate, and with a good reproducibility, and can be used for the quality control of the granules.% 目的建立止咳枇杷糖浆中齐墩果酸及熊果酸的含量测定方法.方法采用Apollo C18色谱柱(4.6mm×250mm,5mm),以乙腈-甲醇-0.5%醋酸铵溶液(67∶12∶21)为流动相,流速1.0mL/min,检测波长为210nm.结果齐墩果酸含量在0.1006~1.5090μg范围内呈良好的线性关
系,r=0.9999,平均回收率为98.73%,RSD为1.05%；熊果酸含量在
0.4028~6.0420μg范围内呈良好的线性关系, r=0.9997,平均回收率为
99.25%,RSD为1.54%.结论该方法简便、结果准确、重现性好,可用于止咳枇杷糖浆的质量控制.
【总页数】2页(P401-402)
【作者】李建芳;黄劲通
【作者单位】广西壮族自治区北海食品药品检验所，广西北海536000;广西恒拓集团仁盛制药有限公司，广西上思535500
【正文语种】中文
【中图分类】R284
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