Summary of Responses received on the report credit risk mode
More than 80R2R3MYB regulatory genes in the genome of Arabidopsis

The Plant Journal (1998)14(3),273–284More than 80R2R3-MYB regulatory genes in the genome of Arabidopsis thalianaI.Romero 1,A.Fuertes 1,M.J.Benito 1,J.M.Malpica 2,A.Leyva 1and J.Paz-Ares 1,*1Centro Nacional de Biotecnologı´a-CSIC,Campus de Cantoblanco,28049-Madrid,Spain,and2Instituto Nacional de Investigaciones Agrarias,ctra.de La Corun ˜a,Km.7,528040-Madrid,SpainSummaryTranscription factors belonging to the R2R3-MYB family contain the related helix-turn-helix repeats R2and R3.The authors isolated partial cDNA and/or genomic clones of 78R2R3-MYB genes from Arabidopsis thaliana and found accessions corresponding to 31Arabidopsis genes of this class in databanks,seven of which were not represented in the authors’collection.Therefore,there are at least 85,and probably more than 100,R2R3-MYB genes present in the Arabidopsis thaliana genome,representing the largest regulatory gene family currently known in plants.In contrast,no more than three R2R3-MYB genes have been reported in any organism from other phyla.DNA-binding studies showed that there are differences but also frequent overlaps in binding specificity among plant R2R3-MYB proteins,in line with the distinct but often related functions that are beginning to be recognized for these proteins.This large-sized gene family may contribute to the regulatory flexibility underlying the developmental and metabolic plasticity displayed by plants.IntroductionTranscription factors play a central role in the regulation of developmental and metabolic programs.Despite the large differences in these programs,existing among organisms from different eukaryotic phyla,their transcrip-tion factors are quite conserved and most of them can be grouped into a few families according to the structural features of the DNA-binding domain they contain.One of these families is that of the R2R3-MYB proteins,whose complexity in plants is addressed in this study.The prototype of this family is the product of the animal c-MYB proto-oncogene,whose DNA-binding domain consists of three related helix-turn-helix motifs of about 50amino acid residues,the so-called R1,R2andReceived 18August 1997;revised 26January 1998;accepted 28January 1998.*For correspondence (fax ϩ33415854506;e-mail jpazares@cnb.uam.es).©1998Blackwell Science Ltd273R3repeats.The repeat most proximal to the N-terminus(R1)does not affect DNA-binding specificity and is missing in oncogenic variants of c-MYB,such as v-MYB,and in the known plant R2R3-MYB proteins (Graf,1992;Lipsick,1996;Lu ¨scher and Eisenman,1990;Martin and Paz-Ares,1997;Thompson and Ramsay,1995).R2R3-MYB proteins belong to the MYB superfamily,which also includes proteins with two or three more distantly related repeats (e.g.of the R1/2type,the progenitor of the R1and R2repeats),and proteins with one repeat,either of the R1/2type (Feldbru ¨gge et al .,1997)or of the R3type (Bilaud et al .,1996;Kirik and Ba ¨umlein,1996).Genes of the MYB superfamily have been found in all eukaryotic organisms in which their presence has been investigated.However,the R2R3-type is not present in Saccharomyces cerevisiae and only 1–3copies of R2R3-MYB genes per haploid genome have been described in organisms from protists and animals (Graf,1992;Lipsick,1996;Lu ¨scher and Eisenman,1990;Thompson and Ramsay,1995).In contrast,preliminary evidence suggest that plants contain a much larger number of these genes (Avila et al .,1993;Jackson et al .,1991;Marocco et al .,1989;Oppenheimer et al .,1991).Little is known about the function of most plant R2R3-MYB genes although,in those few cases in which functions are known,these are different from those of their animal counterparts,which are mostly associated with the control of cell proliferation,prevention of apoptosis,and commit-ment to development (Graf,1992;Lipsick,1996;Lu ¨scher and Eisenman,1990;Martin and Paz-Ares,1997;Taylor et al .,1996;Thompson and Ramsay,1995;Toscani et al .,1997).Thus,most members of the plant R2R3-MYB family with known functions have been implicated in the regula-tion of the synthesis of different phenylpropanoids (Cone et al .,1993;Franken et al .,1994;Grotewold et al .,1994;Moyano et al .,1996;Paz-Ares et al .,1987;Quattrocchio et al .,1993;Quattrocchio,1994;Sablowski et al .,1994;Solano et al .,1995a).Phenylpropanoids are a large class of chemically different metabolites originating from phenylalanine,which includes flavonoids,coumarins and cinnamyl alcohols among others (Hahlbrock and Scheel,1989).Despite their chemical diversity,these compounds are biosynthetically related as their synthesis does include common enzymatic steps.Other functions associated with members of the plant R2R3-MYB gene family include the control of cell differentiation (Noda et al .,1994;Oppenheimer et al .,1991)and the mediation of responses to signalling molecules such as salicylic acid and the phytohormones abscisic acid (ABA)and giberellic acid274I.Romero et al.(GA)(Gubler et al .,1995;Urao et al .,1993;Yang and Klessig,1996).Sequence specific DNA-binding has been demonstrated for several R2R3-MYB proteins,in agreement with their role in transcriptional control (Biedenkapp et al .,1988;Grotewold et al .,1994;Gubler et al .,1995;Howe and Watson,1991;Li and Parish,1995;Moyano et al .,1996;Sablowski et al .,1994;Sainz et al .,1997;Solano et al .,1995a;Solano et al .,1997;Stober-Gra ¨sser et al .,1992;Urao et al .,1993;Watson et al .,1993;Yang and Klessig,1996).The information available indicates that these proteins bind to one or more of the following types of site:I,CNGTTR;II,GKTWGTTR;and IIG,GKTWGGTR (where N indicates A,G,C or T;K,G or T;R,A or G;W,A or T).For instance,animal R2R3-MYB proteins recognize type I sequences (Biedenkapp et al .,1988;Howe and Watson,1991;Stober-Gra ¨sser et al .,1992;Watson et al .,1993),the ZmMYBP (also known as P)proteins bind to type IIG sequences,the ZmMYBC1(also known as C1)and AmMYB305proteins bind to both type II and type IIG,and the PhMYB3protein can bind to types I and II (Grotewold et al .,1994;Sablowski et al .,1994;Sainz et al .,1997;Solano et al .,1995a;Solano et al .,1997).Recent studies with protein PhMYB3from Petunia,including molecular modelling based on the solved structure of the mouse c-MYB protein (MmMYB),have highlighted the importance of residues Lys67,Leu71,Lys121and Asn122in determining recognition specificity (Ogata et al .,1994;Solano et al .,1997).These residues are fully conserved in all known plant R2R3-MYB proteins.In contrast,protein AtMYBCDC5,which has two R1/2-type repeats and does not conserve these residues,has a completely different speci-ficity (CTCAGCG,Hirayama and Shinokazi,1996).To evaluate the number of R2R3-MYB genes in plants,and as a first step towards determining the full range of functions associated with these genes using a reverse genetic approach,we have carried out a PCR-based systematic search for R2R3-MYB genes in the model species Arabidopsis thaliana .We estimate that it contains at least 85,and probably more than 100R2R3-MYB genes,representing the largest gene family of regulatory genes described thus far in any plant species.In addition,we have investigated the DNA-binding specificity of representative R2R3-MYB proteins and have shown that there may be differences but also considerable similarities in binding specificitiy between R2R3-MYB proteins,particularly among members of the same phylogenetic group,which is in agreement with the recognizable functional relationships between the members of the R2R3-MYB family.ResultsIsolation of R2R3-MYB clonesAll known plant R2R3-MYB proteins contain highly con-served stretches of amino acid residues within the©Blackwell Science Ltd,The Plant Journal ,(1998),14,273–284Figure 1.Consensus amino acid sequence of the two repeats comprising the DNA binding domain of plant R2R3-MYB proteins as described by A´vila et al .(1993),and oligonucleotide mixtures used in the isolation of the R2R3-MYB genes (N1–6and C1–3).Upper case indicates residues fully conserved in all proteins used to derive the consensus.Lower case indicates residues identical in at least 80%of the proteins.Other symbols are:ϩ,basic amino acid;–,acidic amino acid;#,hydrophobic amino acid.New sequences (published since this alignment,see Figure 2)have not altered this consensus sequence in the regions from which the oligonucleotide sequences were derived,with the exception of PhMYBAn2which has a D/A substitution in the region corresponding to oligonucleotide mixtures C1-C3,although they have increased the variability of residues in variable positions.This variability was taken into account in the design of the oligonucleotide mixtures (R ϭA ϩG,Y ϭC ϩT,S ϭG ϩC,D ϭA ϩG ϩT,N ϭA ϩG ϩC ϩT)and so the oligonucleotide mixtures should have recognized all the more recent additions to the R2R3MYB gene family.recognition helices of the R2and R3repeats from which R2R3-MYB -specific mixtures of oligonucleotides can be derived (Avila et al .,1993;Figure 1).These oligonucleotide mixtures do not recognize the AtMYBCDC5gene encoding a MYB protein with two highly divergent repeats of the R1/2-type (Hirayama and Shinokazi,1996;Lipsick,1996).To search for R2R3-MYB genes,we first prepared cDNA and genomic DNA libraries (of 1000and 3000clones,respectively)enriched in these genes using PCR with R2R3-MYB -specific oligonucleotides.Sequencing of all the different clones present in each of these libraries (for details,see Experimental procedures),revealed that 36and 74different R2R3-MYB genes were represented in the cDNA and genomic DNA libraries,respectively,and that 32were represented in both libraries.A total of 78different R2R3-MYB genes were therefore represented in our collec-tion.A computer search revealed that there were 31R2R3-MYB genes from Arabidopsis described in databanks,of which seven were not represented in the set of 78isolated in this study.There are,therefore,at least 85(78ϩ7),and probably more than 100(78ϫ31/24,see Experimental procedures)R2R3-MYB genes in the Arabidopsis thaliana genome.More than half of the R2R3-MYB genes identified in this study were characterized only at the genomic DNA level,raising the possibility that many of these R2R3-MYBThe R2R3-MYB gene family in Arabidopsis275genomic sequences might represent pseudogenes rather than active genes.However,in no case was the reading frame of the exonic sequences(represented in the genomic clones)prematurely terminated.In addition,the numberof fully conserved residues in plant R2R3-MYB proteins is the same independently of whether those protein sequences from the R2R3-MYB genes characterized onlyat the genomic DNA level are considered in the estimation. On the other hand,pseudogenes usually show higher rates of non-synonymous substitutions(Kns)relative to synonymous substitutions(Ks)than active genes(Satta, 1993).We calculated the Kns/Ks ratio for all possible pairsof R2R3-MYB genes in this population and these ratios were compared to those in the population of R2R3-MYB genes known to be expressed(i.e.those for which a cDNA clone was available),using the method of Nei and Gojobori (1986).The Kns/Ks values in the two populations(Kns/Ksin genomic DNA population:0.393Ϯ0.016;Kns/Ks in cDNA population:0.392Ϯ0.115)were not significantly differentin a t-test(Pϭ0.83ജ0.10).Collectively,these data are in agreement with the conclusion that most,if not all,plantR2R3-MYB sequences represent active genes.Phylogenetic analysis of R2R3-MYB proteinsA phylogram of R2R3-MYB proteins was constructed with the neighbor-joining method(Saitou and Nei,1987)using the sequences of the proteins in Figure2(except HvMYB33, LeMYB1,AtMYB67,AtMYB41and AtMYB45;Figure3). Three major groups were distinguished in the phylogram, A,B and C(Figure3).The bootstrap support for the node corresponding to groupC was not very high(30%),perhaps due to the short size of the sequences used.However, when the analysis was made using the whole R2R3-MYB domain from the proteins for which this sequence was available,the bootstrap support of this node was more than75%(see Figure3).In addition,the existence of the three groups was also supported by the tree constructed using parsimony(Eck and Dayhoff,1966)(not shown)andby the different intron/exon structure of the genes encoding the proteins of each group,with the exception of AtMYB67 (see Figure3).Group A(accounting for about10%of the A.thaliana proteins),which also includes the animal and protist R2R3-MYB proteins,represents genes with no intronin the region sequenced,with the exception of AtMYB1 which has an intron at position1.Group B(5%of the A. thaliana proteins)represents proteins encoded by genes with an intron at position3.Finally,group C(85%of A. thaliana proteins)contains genes with an intron at position 2.As shown below(see Discussion),this classification is also in agreement with the data on DNA-binding specificityof R2R3-MYB proteins,as similarities in this property were usually higher between proteins belonging to the same group than between proteins belonging to different groups.©Blackwell Science Ltd,The Plant Journal,(1998),14,273–284Each group,particularly group C,can be further subdivided into subgroups of more closely related members.Many of these subgroups contain R2R3-MYB proteins from other plant species(although the search for this type of MYB genes in these species has not been exhaustive),consistent with the high functional similarity of regulatory systems among plants(Benfey and Chua,1989).DNA-binding specificity of representative R2R3-MYB proteinsTo evaluate the degree of similarity in DNA binding specificity between different Arabidopsis R2R3-MYB proteins,we isolated cDNA clones containing the entire coding region of four representative R2R3-MYB proteins, AtMYB15,AtMYB77,AtMYB84and AtMYBGl1(see Methods).Full length and deletion derivatives of these proteins were produced by in vitro transcription and translation.To determine their DNA-binding specificity, an EMSA(electrophoretic mobility shift assay)-based random-site selection procedure was used(Blackwell and Weintraub,1990;Solano et al.,1995a).Selection experi-ments were performed with two oligonucleotide mixtures, OI and OII,which had a partially random core sequence representing the three types of sites defined for R2R3-MYB proteins:OI,type I;OII,types II and IIG(Biedenkapp et al., 1988;Grotewold et al.,1994;Gubler et al.,1995;Howe and Watson,1991;Li and Parish,1995;Moyano et al.,1996; Sablowski et al.,1994;Sainz et al.,1997;Solano et al., 1995a;Solano et al.,1997;Stober-Gra¨sser et al.,1992;Urao et al.,1993;Watson et al.,1993;Yang and Klessig,1996; Figure4;see Introduction).In fact,the nucleotides(or their counterparts in the complementary strand)present in the non-randomized positions(–2,ϩ1andϩ3)are contacted by residues fully conserved in all plant R2R3-MYB proteins (Leu71,Lys121and Asn122,respectively,in PhMYB3;the G in the complementary strand of position–2in type I targets is contacted by another fully conserved residue, Lys67(Solano et al.,1997).AtMYB15and AtMYB84bound the partially randomized oligonucleotide mixture OII and,to a lesser extent,the OI oligonucleotide mixture,and the reciprocal was true with a carboxy-terminal deletion derivative of AtMYB77 (AtMYB77∆C1)which bound better to OI(data not shown). AtMYB77∆C1was used because the full size protein had lower binding affinity,as is the case with other R2R3-MYB proteins(PhMYB3and MmMYB)(Ramsay et al.,1992; Solano et al.,1995a).In contrast,neither AtMYBGl1nor its carboxy-terminal deletion derivatives showed detectable binding to either of these oligonucleotide mixtures(not shown).A similar result was obtained with an increased amount of probe and/or a decreased amount of non-specific competitor DNA,independently of the type of probe used,the partially randomized oligonucleotide276I.Romero et al.mixtures OI and OII,or a fully randomized mixture (O,data not shown).Protein phosphatase treatments,which have been shown to increase binding affinity of one R2R3-MYB protein (Moyano et al .,1996),were also ineffective (not shown).Collectively,these data suggest limited in vitro DNA-binding affinity for this protein.It is possible that low DNA-binding affinity is an intrinsic property of AtMYBGl1and that it might be increased in vivo after interaction(s)with other protein(s).For example,there is evidence that maize C1protein (ZmMYBC1),which also shows low bind-ing affinity in vitro (Sainz et al .,1997),requires an inter-action with a second protein (the MYC protein R,Goff et al .,1992)to activate flavonoid biosynthetic genes.A similar interaction is possibly necessary for the activity of AtMYBGl1in vivo (Lloyd et al .,1992).©Blackwell Science Ltd,The Plant Journal ,(1998),14,273–284After four cycles of enrichment,oligonucleotides selected by the R2R3-MYB proteins were cloned and sequenced.In all instances,despite using two target oligonucleotide mixtures,only one type of sequence was recovered for each protein,indicating strong preference for one of the types of sequences (Figure 4).For instance,in the case of protein AtMYB77∆C1,which preferred type I sequences,the sequences selected from oligonucleotide OII were also of type I (generated in variable positions of OII,not shown)and the reciprocal was true for proteins AtMYB15and AtMYB84(not shown).These results argue against a bias in the binding site selection experiments due to the use of partially degenerated oligonucleotide mixtures,although this possibility cannot be fully excluded.Next,we used oligonucleotides representing the definedThe R2R3-MYB gene family in Arabidopsis 277optimal target sites and mutants of these sites in binding experiments with each of the above Arabidopsis proteins and with carboxy-terminal deletion derivatives of PhMYB3(PhMYB3∆C1),AmMYB305(AmMYB305∆C1)and MmMYB (MmMYB ∆C2R1;Solano et al .,1997)as controls (Figure 5a).The results of these experiments agreed with those from site selection experiments,but revealed that AtMYB77∆C1also recognised certain type II sequences,although with reduced affinity compared to that for type I sequences.In addition,they also showed specific DNA binding affinity for AtMYBGl1,as it could weakly bind to oligonucleotide II-1.In an apparent discrepancy with binding site selection experiments,protein AtMYB77∆C1bound better to the oligonucleotide containing one of the optimal binding sites of PhMYB3(MBSI,oligonucleotide I-1;Solano et al .,1995a)than to that containing its deduced optimal binding sequence (oligonucleotide I-2).Discrepancies between a binding site selection derived sequence with the optimal binding site have also been reported for MADS box proteins (Riechmann et al .,1996).A difference between the two oligonucleotides (I-1and I-2)is that I-1is flanked by three extra As,which would increase its ability to bend,a property known to greatly influence binding by DNA-distorting/bending proteins,such as R2R3-MYB proteins and MADS proteins (Parvin et al .,1995;Riechmann et al .,1996;Solano et al .,1995b;Thanos and Maniatis,1992).To test whether this difference could be the cause of the preference of AtMYB77∆C1for oligonucleotide I-1versus I-2,DNA binding experiments were conducted with new oligonucleotides in which the three extra As of oligonucleo-tide I-1had been removed.The binding by AtMYB77∆C1to this deletion version of I-1(I-1∆)was similar to that obtained for the oligonucleotide derived from binding siteFigure 2.Deduced amino acid sequences of Arabidopsis R2R3-MYB proteins.For comparison,the sequences of R2R3-MYB proteins from other plant species and from representative organisms of other phyla are also given.The region shown is that flanked by the sequences used to derive the oligonucleotide mixtures shown in Figure 1.The clones corresponding to AtMYB41and to AtMYB45did not encode the carboxy-terminal part of their sequence due to mispriming events.For protein (and gene)names,a standardized nomenclature has been used (Martin and Paz-Ares,1997)whereby the name of each protein includes a two-letter prefix as species identifier,the term MYB,and then a term describing the particular family member.The codes for the species identifier are:Am,Antirrhinum majus ;At,Arabidopsis thaliana ;Cp,Craterostigma plantagineum ;Dd,Dictyostelium discoideum ;Dm,Drosophila melanogaster ;Gh,Gossypium hirsutum ;Hv,Hordeum vulgare ;Le,Lycopersicon esculentum ;Mm,Mus musculus ;Nt,Nicotiana tabacum ;Os,Oryza sativa ;Ph,Petunia hybrida ;Pm,Picea mariana ;Pp,Physcomitrella patens ;Ps,Pisum sativum ;Xl,Xenopus laevis ;Zm ,Zea mays.As family member identifier we have always used a number except where the previously given name was based on functional information,such as the phenotype of mutants (e.g.the Gl1(Glabrous1)protein from Arabidopsis is named AtMYBGl1).Thus,all the genes identified in this study have been given a standardized number independent of whether a different non-standardized name has been given by other authors.This has occurred in the following cases:AtMYB13,also named AtMYBlfgn (accession number Z50869);AtMYB15,also named Y19(X90384);AtMYB16,also named AtMIXTA (X99809);AtMYB23,also named AtMYBrtf (Z68158);AtMYB31,also named Y13(X90387);AtMYB44,also named AtMYBR1(Z54136);AtMYB77,also named AtMYBR2(Z54137).In addition,the following R2R3-MYB genes,which were not identified in this study,were renamed (with the agreement of the authors who first described them):AtMYB101(M1);AtMYB102(M4).AtMYB90is described in the EMBL databank as an anonymous EST (H76020).The column on the right of the amino acid sequence gives the accession number from which the sequences were derived.The accession numbers of the cDNAs encoding the full-size proteins AtMYB15,AtMYB77and AtMYB84are Y14207,Y14208and Y14209,respectively.In case of PhMYBAn2,the sequence was copied directly from Quattrochio (1994).The second column shows the position of the intron interrupting that part of coding sequence represented in the figure:–,unknown;0,no intron;the localization of introns 1,2and 3is shown relative to the consensus sequence.The third column shows the type of clone isolated in this study:a,cDNA clone;b,genomic clone.Other letters in this column indicate that the sequence shown in the figure was previously described in databanks or published (c)or that only part of the sequence shown was previously described (d).Two additional sequences (accession numbers H36793and T42245),each corresponding to a novel Arabidopsis R2R3-MYB gene,were found in the EST databank,but are not represented in the figure because they were incomplete.These sequences were,however,used for the estimation of the size of the R2R3-MYB gene family.Asterisks indicate proteins for which the sequence of the whole R2R3-MYB domain is known.Symbols in the consensus sequence are as in Figure 1.©Blackwell Science Ltd,The Plant Journal ,(1998),14,273–284selection experiments (Figure 5b).This result underscored the importance of DNA conformational properties in bind-ing by transcriptional factors.DiscussionGenes of the R2R3-MYB family are quite widespread in eukaryotes,with the exception of yeast,and in plants the number of these genes is especially high.Whereas no more than three R2R3-MYB genes have been described in any organisms from other eukaryotic phyla,here we isol-ated partial cDNA and/or genomic clones corresponding to 78different R2R3-MYB genes from Arabidopsis and estimated that there are probably more than 100R2R3-MYB genes in this species.The different size of regulatory gene families in different groups of eukaryotes,a situation which is not exclusive for R2R3-MYB genes (for instance,see the case of MADS box proteins;Theissen et al .,1996),might reflect major differences in developmental and meta-bolic programs generated during evolution of these groups,which largely involved a different use of pre-existing regu-latory systems rather than the generation of new systems (Martin and Paz-Ares,1997).According to recent estimates on the number of genes in Arabidopsis (16000–43000;Gibson and Sommerville,1993),members of the R2R3-MYB family would represent at least 0.2–0.6%of the total Arabidopsis genes,the largest proportion of genes thus far assigned to a single regulatory gene family (and even to a gene family encoding any type of protein)in plants.In other types of eukaryotes there are families of equal,or even larger,size;for instance,it is estimated that genes encoding zinc-finger proteins represent about 1%of the human genes (Hoovers278I.Romero et al.et al .,1992)and,in Caenorhabditis elegans ,about 0.4%of its genes contain homeoboxes (Bu ¨rglin,1995).However,in these families overall sequence conservation is very low and variability in DNA-binding specificity is high (Klug and Schwabe,1995;Treisman et al .,1992).In contrast,members of the plant R2R3-MYB family share higher amino acid sequence similarity,particularly in their recogni-tion helices (Figure 1)and display considerable DNA-recognition similarities (Figures 3and 5).These similarities in recognition specificity are par-ticularly noticeable between members of thesame ©Blackwell Science Ltd,The Plant Journal ,(1998),14,273–284phylogenetic group,although in some cases overlaps in binding specificity between members belonging to differ-ent groups have been observed (Figures 3and 5).Thus,in the cases studied here or elsewhere (Biedenkapp et al .,1988;Grotewold et al .,1994;Gubler et al .,1995;Howe and Watson,1991;Li and Parish,1995;Moyano et al .,1996;Sablowski et al .,1994;Sainz et al .,1997;Solano et al .,1995a,1997;Stober-Gra ¨sser et al .,1992;Urao et al .,1993;Watson et al .,1993;Yang and Klessig,1996)members from group A (including both those from plants and from organisms from other phyla)prefer (or bindThe R2R3-MYB gene family in Arabidopsis 279to)a type I sequence,members of group B bind equally well to both type I and type II,and most members of group C prefer (or bind to)a type IIG.Possible exceptions are the proteins from group C AtMYB2,reported to bind type I sequences (Urao et al .,1993),and GLABROUS1(AtMYBGl1),which only bound to a type II sequence (Figure 5)although,in the first case,binding to IIG sequences was not studied and,in the second case,binding site selection experiments failed to provide information on its optimal binding site (see Results).However,it is striking that the only sequence bound by AtMYBGl1(AAAGTTAGTTA)perfectly conforms to the sequence of gibberellic acid responsive elements,and gibberellic acid is known to affect the AtMYBGl1-controlled trait trichome formation (Oppenheimer et al .,1991;Telfer et al .,1997).In line with these similarities in binding specificity,and despite the fact that target selectivity is usually also influenced by interactions with other factors,most of the R2R3-MYB proteins studied so far,which are scattered throughout groups B and C,have been implicated in the control of phenylpropanoid biosynthetic genes (Cone et al .,1993;Franken et al .,1994;Grotewold et al .,1994;Moyano et al .,1996;Paz-Ares et al .,1987;Quattrocchio et al .,1993,1994;Sablowski et al .,1994;Solano et al .,1995a;Figure 3).Nevertheless,there are some R2R3-MYB proteins that have been implicated in other functions,including the control of cell differentiation and the mediation of plant responses to several signal molecules (Gubler et al .,1995;Noda et al .,1994;Oppenheimer et al .,1991;Urao et al .,1993;Yang and Klessig,1996).TargetFigure 3.Phylogenetic tree of the R2R3-MYB family using the neighbor-joining method (Saitou and Nei,1987).The phylogram shown was constructed with the sequences given in Figure 2,except HvMYB33,LeMYB1,AtMYB67,AtMYB41and AtMYB45.The first two were excluded because they were the only ones out of the 57known complete-MYB-domain sequences which grouped differently (with bootstrap support Ͼ50%)depending on whether the complete MYB domains or the portion characterized in this study was used in the calculations.Protein AtMYB67was the only one which was not grouped with the other proteins encoded by genes with the same intron/exon structure.Proteins AtMYB41and AtMYB45were not used because only partial sequence data were available,although their probable position in the phylogram,inferred from a tree constructed also using their incomplete sequences (not shown),is indicated in the tree with dashed lines.Exclusion of these five proteins increased the bootstrap support of the major nodes (not shown).Names of R2R3-MYB proteins from non-plant species are shown in red.The three major nodes,A,B and C,are denoted.Numbers (0,1,2or 3)in some branches indicate the type of intron in the cloned portion of the genes encoding proteins originating from the respective branch,as far as the genes for which this information is available are concerned (Figure 2).Nodes with high bootstrap support are indicated (empty symbols,bootstraps Ͼ50%;filled symbols,bootstraps Ͼ75%).Circles refer to bootstraps data corresponding to the represented tree.Squares refer to bootstraps data corresponding to the tree constructed with the sequence of the whole MYB domain of the proteins for which this information was available (Figure 2).The known functions associated with some plant R2R3-MYB proteins are indicated:Ph,regulation of phenylpropanoid biosynthetic genes (proteins ZmMYBC1,ZmMYBPl,ZmMYBP ,ZmMYB38,ZmMYB1,AmMYB305,AmMYB340,PhMYBAn2;PhMYB3,Cone et al .,1993;Franken et al .,1994;Grotewold et al .,1994;Moyano et al .,1996;Paz-Ares et al .,1987;Quattrocchio et al .,1993;Quattrocchio,1994;Sablowski et al .,1994;Solano et al .,1995a);CD,control of cell differentiation (proteins AtMYBGl1and AmMYBMx,Noda et al .,1994;Oppenheimer et al .,1991);SA,GA and ABA,involved in signal transduction pathway,respectively,salicylic acid (gene NtMYB1;Yang and Klessig,1996),gibberellic acid (proteins HvMYBGa,Gubler et al .,1995)and abscisic acid (proteins AtMYB2and ZmMYBC1;Hattori et al .,1992;Urao et al .,1993).Capital letters are used when the functions associated are based on genetic evidence (i.e.analysis of mutants).Also indicated is the available information on DNA-binding specificity of some of the R2R3-MYB proteins,(arrowheads indicate the proteins examined in this study):I,CNGTTR (proteins MmMYB,MmMYBA,MmMYBB,DdMYB,AtMYB1,AtMYB2,AtMYB77,PhMYB3,HvMYBGa,NtMYB1;Biedenkapp et al .,1988;Howe and Watson,1991;Solano et al .,1995a;Stober-Gra ¨sser et al .,1992;Urao et al .,1993;Watson et al .,1993);II,GTTWGTTR (proteins PhMYB3,HvMYBGa,AmMYB305,ZmMYBC1,AtMYBGl1;Gubler et al .,1995;Sainz et al .,1997;Solano et al .,1995a;Solano et al .,1997);IIG,GKTWGGTR (proteins AmMYB305,AmMYB340,ZmMYBP ,ZmMYBC1,AtMYB6,AtMYB7,AtMYB15,AtMYB84,NtMYB1;Grotewold et al .,1994;Li and Parish,1995;Moyano et al .,1996;Sablowski et al .,1994;Sainz et al .,1997;Solano et al .,1995a;Yang and Klessig,1996)(where N indicates A or G or C or T;K,G or T;R,A or G;W,A or T).Capital letters are used in those cases in which the sequences are known to be the optimal binding site.When a given protein is able to bind to more than one type of site,the size of the letter reflects the relative binding affinity for these sites.©Blackwell Science Ltd,The Plant Journal ,(1998),14,273–284genes of these latter R2R3-MYB genes are mostly unknown,thus precluding definite conclusions about whether they are functionally related between themselves or indeed with the R2R3-MYB genes regulating phenyl-propanoid biosynthetic genes.However,the signal molecules salicylic acid,ABA and GA influence,among others,the expression of phenylpropanoid biosynthetic genes,in several instances through cis -acting elements resembling R2R3-MYB binding sites (Dixon and Paiva,1995;Hahlbrock and Scheel,1989;Hattori et al .,1992;Sablowski et al .,1994;Shirasu et al .,1997;Weiss et al .,1990,1992).In addition,GA also affects trichome forma-tion,another trait under the control of an R2R3-MYB gene,AtMYBGl1(Telfer et al .,1997).Moreover,the MIXTA gene (AmMYBMx )controls the specialized shape of inner epidermal petal cells of Antirrhinum flowers,and these changes in cell shape correlate with changes in the cell wall,a structure containing phenylpropanoid derivatives (Noda et al .,1994).The number of R2R3-MYB genes with distinct but related functions might therefore be extraordinarily high,particularly with regard to the regulation of different phenylpropanoid biosynthetic genes,although some of these genes could also (or alternatively)act on other types of targets (e.g.the barley gibberellic acid induced α-amy gene is a likely target of HvMYBGa,Gubler et al .,1995).In any case,the broad (phylogenetic)distribution of the R2R3-MYB genes for which there is evidence of their involvement in the regulation of phenylpropanoid metabolism,suggests that a very early plant-specific R2R3-MYB ancestor already had this function,and that。
ResultsSummary

13 October 2015Irish Responses October 2015 Bank Lending Survey___________________________________________________________________________Results Summary:∙Credit standards were unchanged on loans to enterprises across all loan categories examined during the third quarter of 2015 and are expected to remain unchanged during the fourth quarter. Overall demand for credit from enterprises increased in the third quarter.∙Credit standards tightened with respect to lending to households for house purchases while remaining unchanged with respect to consumer credit and other lending. Household loan demand for house purchases remained unchanged in the third quarter, while increasing for consumer credit and other lending.∙Access to retail and wholesale funding sources was mostly unchanged during the third quarter of 2015 and is expected to remain unchanged during the fourth quarter of 2015. The EAPP was reported to have improved market financing conditions, but not to have had an impact on credit standards or terms and conditions to enterprises or to households.I. EnterprisesAs detailed in Table 1, credit standards were unchanged on loans to enterprises across all five categories examined during the third quarter of 2015. Consistent with this, all of the factors impacting credit standards were reported as unchanged, as were the terms and conditions offered on new loans or credit lines to enterprises.Table 1: Changes in Credit Standards on Loans to EnterprisesQ4 2014 Q1 2015 Q2 2015 Q3 2015 Enterprises: Overall 3.25 3.00 3.00 3.00Small/medium enterprises 3.25 3.00 3.00 3.00Large enterprises 3.00 3.00 3.00 3.00Short-term loans 3.25 3.00 3.00 3.00Long-term loans 3.00 3.00 3.00 3.00Key: 1 = tightened considerably, 2 = tightened somewhat, 3 = remained basically unchanged, 4 = eased somewhat, 5 = eased considerably.For the third successive quarter, the share of loan applications from enterprises that were completely rejected has remained unchanged. Overall loan demand from enterprises increased for the seventh successive quarter albeit at a lower rate than during late-2014/early-2015 (Table 2). Loan demand increased from SMEs and for both short- and long-term loans, while loan demand from large enterprises remained unchanged. Regarding the factors affecting loan demand, there was an increase in demand for fixed investment, which corresponds with demand for long-term loans. The other main driver of loan demand was foraccumulation of working capital. All other demand factors remained unchanged in the third quarter (Figure 1).Table 2: Changes in Loan Demand from EnterprisesQ4 2014 Q1 2015 Q2 2015 Q3 2015 Enterprises: Overall 3.75 3.50 3.25 3.25Small/medium enterprises 4.00 3.50 3.25 3.25Large enterprises 3.75 3.25 3.25 3.00Short-term loans 3.50 3.50 3.25 3.25Long-term loans 3.75 3.75 3.50 3.25Key: 1 = decreased considerably, 2 = decreased somewhat, 3 = remained basically unchanged, 4 = increased somewhat, 5 = increased considerably.Looking forward to the fourth quarter of 2015, credit standards are expected to remain unchanged in respect of loans to enterprises across all five loan categories examined. Loan demand is expected to remain constant for large enterprises once again, but to increase further overall and for SMEs.Figure 1: Changes in Loan Demand from Enterprises and Factors affecting Loan Demand.Key: A response less than 3 indicates that a factor contributed to a reduction in demand while a response greater than 3 indicates that a factor contributed to increased demand.II.HouseholdsCredit standards on loans to households for house purchases tightened during the third quarter of 2015, having tightened in the first quarter and eased marginally in the second quarter. Lending terms and conditions were also reported to have tightened, including loan-to-value ratios, other loan size limits and the margins on average loans to households for house purchase. Competition from other banks or from non-banks continues to have no impact on credit standards for house purchases (Figure 2).Figure 2: Change in Credit Standards: Lending to Households for House Purchases and Factors affecting Credit Standards.Key: A response less than 3 indicates that a factor contributed to a tightening in credit standards while a response greaterthan 3 indicates that a factor contributed to an easing in credit standards.Credit standards on loans to households with respect to consumer credit were reported to be unchanged in the third quarter, with all factors affecting credit standards reported to be unchanged. There was a reported tightening in the terms and conditions on loans to households for consumer credit in terms of loans margins. Overall, for the fourth quarter, the respondents reported an expected tightening in credit standards for house purchase and easing in credit standards for consumer credit.Table 3: Changes in Loan Demand from HouseholdsQ4 2014 Q1 2015 Q2 2015 Q3 2015 Loans for House Purchases 4.00 3.80 2.80 3.00Loans for Consumer Credit 3.60 3.40 3.60 3.60Key: 1 = decreased considerably, 2 = decreased somewhat, 3 = remained basically unchanged, 4 = increased somewhat, 5 = increased considerablyTable 3 shows that demand for mortgage lending remained unchanged in the third quarter, having fallen in the second quarter. This represented the first reported decrease in demand for loans for house purchase since the first quarter of 2012. There was a considerable increase in demand during 2014, peaking in the third quarter of 2014.Lower demand for mortgage lending in the third quarter of 2015 was attributed to the regulatory/fiscal regime of housing markets. Factors which had acted to increase demand for mortgages included consumer confidence and house price developments/prospects for the housing market.Demand for loans for household credit continued to increase in the third quarter, driven by spending on durable consumer goods and higher levels of consumer confidence. The share of household loan applications completely rejected remained unchanged in the third quarter of 2015, both in terms of consumer credit and loans for house purchases. Loan demand from households is expected to increase across both categories during the fourth quarter.III. Ad-Hoc QuestionsThe October round of the BLS included four ad-hoc questions examining (i) access to retail and wholesale funding sources and (ii - iv) the impacts of the Expanded Asset Purchase Programme (EAPP).According to responses received, access to retail funding has remained largely unchanged over the last three months. With regard to wholesale funding, there was a reported easing of market access for securitisation of home loans over the last three months and an expected further easing in the next three months.It was reported that the EAPP improved bank profitability and improved market financing conditions, particularly with regard to covered bonds and unsecured bank bonds. The EAPP is expected to continue to improve market financing over the next three months.The additional liquidity arising from the EAPP was reported to have had little impact over the past six months. Additionally, it is also expected to have little impact over the next six months. Regarding the impact of the EAPP on bank lending behaviour, it was reported that there was no impact in terms of either credit standards or terms and conditions. This followed for both enterprises and households.。
Glossary_of_Terms_英语文学术语解释

Glossary of TermsA |B |C |D |E |F |G |H |I |J |K |L |M |N |O |P |Q |R |S |T |U |V |W | ZAAbstract: Used as a noun, the term refers to a short summary or outline of a longer work. As an adjective applied to writing or literary works, abstract refers to words or phrases that name things not knowable through the five senses.Examples of abstracts include the Cliffs Notes summaries of major literary works. Examples of abstract terms or concepts include "idea," "guilt" "honesty," and "loyalty." (Compare with Concrete.)Absurd, Theater of the: See Theater of the AbsurdAbsurdism: See Theater of the AbsurdAccent: The emphasis or stress placed on a syllable in poetry. Traditional poetry commonly uses patterns of accented and unaccented syllables (known as feet) that create distinct rhythms. Much modern poetry uses less formal arrangements that create a sense of freedom and spontaneity.The following line from William Shakespeare's Hamlet:"To be or not to be: that is the question"has five accents, on the words "be," "not," "be," and "that," and the first syllable of "question." (See also Cadence, Foot, Measure, Meter, poem, Poetics, Poetry, Scansion, Sprung Rhythm, Verse, and Versification.)Act: A major section of a play. Acts are divided into varying numbers of shorter scenes. From ancient times to the nineteenth century plays were generally constructed of five acts, but modern works typically consist of one, two, or three acts.Examples of five-act plays include the works of Sophocles and Shakespeare, while the plays of Arthur Miller commonly have a three-act structure. (Compare with Scene.) (See also drama.)Acto: A one-act Chicano theater piece developed out of collective improvisation. Actos were performed by members of Luis Valdez's Teatro Campesino in California during the mid-1960s.Aestheticism: A literary and artistic movement of the nineteenth century. Followers ofthe movement believed that art should not be mixed with social, political, or moral teaching. The statement "art for art's sake" is a good summary of aestheticism. The movement had its roots in France, but it gained widespread importance in England in the last half of the nineteenth century, where it helped change the Victorian practice of including moral lessons in literature.Oscar Wilde is one of the best-known "aesthetes" of the late nineteenth century. (See also Decadents.)Affective Fallacy: (Also known as Sympathetic Fallacy.) An error in judging the merits or faults of a work of literature. The "error" results from stressing the importance of the work's effect upon the reader —that is, how it makes a reader "feel" emotionally, what it does as a literary work —instead of stressing its inner qualities as a created object, or what it "is."The affective fallacy is evident in Aristotle's precept from his Poeticsthat the purpose of tragedy is to evoke "fear and pity" in its spectators.Age of Johnson: (Also known as Age of Sensibility). The period in English literature between 1750 and 1798, named after the most prominent literary figure of the age, Samuel Johnson. Works written during this time are noted for their emphasis on "sensibility," or emotional quality. These works formed a transition between the rational works of the Age of Reason, or Neoclassical period, and the emphasis on individual feelings and responses of the Romantic period.Significant writers during the Age of Johnson included the novelists Ann Radcliffe and Henry Mackenzie, dramatists Richard Sheridan and Oliver Goldsmith, and poets William Collins and Thomas Gray. (Compare with Neoclassicismand romanticism.)Age of Reason: See NeoclassicismAge of Sensibility: See Age of JohnsonAgrarians: A group of Southern American writers of the 1930s and 1940s who fostered an economic and cultural program for the South based on agriculture, in opposition to the industrial society of the North. The term can refer to any group that promotes the value of farm life and agricultural society.Members of the original Agrarians included John Crowe Ransom, Allen Tate, and Robert Penn Warren.Alexandrine Meter: See MeterAllegory: A narrativetechnique in which characters representing things or abstractideas are used to convey a message or teach a lesson. Allegory is typically used to teach moral, ethical, or religious lessons but is sometimes used for satiric or political purposes.Examples of allegorical works include Edmund Spenser's The Faerie Queene and John Bunyan's The Pilgrim's Progress.(See also Exemplumand Fable.)Alliteration: A poetic device where the first consonant sounds or any vowel sounds in words or syllables are repeated.The following description of the Green Knight from the anonymous Sir Gawain and the Green Knight gives an example of alliteration:And in guise all of green, the gear and the man:A coat cut close, that clung to his sidesAn a mantle to match, made with a liningOf furs cut and fitted — the fabric was noble....(Compare with Assonanceand rhyme.) (See also poem, Poetics, Poetry, Verse, and Versification.)Allusion: A reference to a familiar literary or historical person or event, used to make an idea more easily understood.For example, describing someone as a "Romeo" makes an allusion to William Shakespeare's famous young lover in Romeo and Juliet.Amerind Literature: The writing and oral traditions of Native Americans. Native American literaturewas originally passed on by word of mouth, so it consisted largely of stories and events that were easily memorized. Amerind proseis often rhythmic like Poetry because it was recited to the beat of a ceremonial drum.Examples of Amerind literature include the autobiographical Black Elk Speaks, the works of N. Scott Momaday, James Welch, and Craig Lee Strete, and the poetry of Luci Tapahonso.Analogy: A comparison of two things made to explain something unfamiliar through its similarities to something familiar, or to prove one point based on the acceptedness of another. Similes and metaphors are types of analogies.Analogies often take the formof an extended simile, as in William Blake's aphorism: "As the caterpillar chooses the fairest leaves to lay her eggs on, so the priest lays his curse on the fairest joys." (Compare with Simileand Metaphor.)Anapest: See FootAngry Young Men: A group of British writers of the 1950s whose work expressed bitterness and disillusionment with society. Common to their work is an anti-hero who rebels against a corrupt social order and strives for personal integrity.The term has been used to describe Kingsley Amis, John Osborne, Colin Wilson, John Wain, and others.Antagonist: The major characterin a narrativeor dramawho works against the heroor protagonist.An example of an evil antagonist is Richard Lovelace in Samuel Richardson's Clarissa, while a virtuous antagonist is Macduff in William Shakespeare's Macbeth.(Compare with protagonist.) (See also anti-hero, conflict.)Anthropomorphism: The presentation of animals or objects in human shape or with human characteristics. The term is derived from the Greek word for "human form."The Fables of Aesop, the animated films of Walt Disney, and Richard Adams's Watership Downfeature anthropomorphic characters. (Compare with Personification.) Anti-hero: A central characterin a work of literature who lacks traditional heroic qualities such as courage, physical prowess, and fortitude. Anti-heros typically distrust conventional values and are unable to commit themselves to any ideals. They generally feel helpless in a world over which they have no control. Anti-heroes usually accept, and often celebrate, their positions as social outcasts.A well-known anti-hero is Yossarian in Joseph Heller's novel Catch-22.(Compare with Antagonist, Hero, and Protagonist.)Antimasque: See MasqueAnti-novel: A term coined by French critic Jean-Paul Sartre. It refers to any experimental work of fictionthat avoids the familiar conventions of the novel. The anti-novel usually fragments and distorts the experience of its characters, forcing the reader to construct the reality of the story from a disordered narrative.The best-known anti-novelist is Alain Robbe-Grillet, author of Le voyeur.Antithesis: The antithesis of something is its direct opposite. In literature, the use of antithesis as a figure of speech results in two statements that show a contrast through the balancing of two opposite ideas. Technically, it is the second portion of the statement that is defined as the "antithesis"; the first portion is the "thesis."An example of antithesis is found in the following portion of Abraham Lincoln's "Gettysburg Address"; notice the opposition between the verbs "remember" and "forget" and the phrases "what we say" and "what they did": "The world will little note nor long remember what we say here, but it can never forget what they did here."Apocrypha: Writings tentatively attributed to an author but not proven or universally accepted to be their works. The term was originally applied to certain books of the Bible that were not considered inspired and so were not included in the "sacred canon."Geoffrey Chaucer, William Shakespeare, Thomas Kyd, Thomas Middleton, and John Marston all have apocrypha. Apocryphal books of the Bible include the Old Testament's Book of Enoch and New Testament's Gospel of Thomas.Apollonian and Dionysian: The two impulses believed to guide authors of dramatic tragedy. The Apollonian impulse is named after Apollo, the Greek god of light and beauty and the symbol of intellectual order. The Dionysian impulse is named after Dionysus, the Greek god of wine and the symbol of the unrestrained forces of nature. The Apollonian impulse is to create a rational, harmonious world, while the Dionysian is to express the irrational forces of personality.Friedrich Nietzche uses these terms in The Birth of Tragedyto designate contrasting elements in Greek tragedy. (Compare with classicismand romanticism.) Apostrophe: A statement, question, or request addressed to an inanimate object or concept or to a nonexistent or absent person.Requests for inspiration from the musesin poetry are examples of apostrophe, as is Marc Antony's address to Caesar's corpse in William Shakespeare's Julius Caesar: "O, pardon me, thou bleeding piece of earth,That I am meek and gentle with these butchers!...Woe to the hand that shed this costly blood!..."(Compare with Monologueand Soliloquy.)Apprenticeship Novel: See BildungsromanArchetype: The word archetype is commonly used to describe an original pattern or model from which all other things of the same kind are made. This term was introduced to literary criticismfrom the psychology of Carl Jung. It expresses Jung's theory that behind every person's "unconscious," or repressed memories of the past, lies the "collective unconscious" of the human race: memories of the countless typical experiences of our ancestors. These memories are said to prompt illogical associations that trigger powerful emotions in the reader. Often, the emotional process is primitive,even primordial. Archetypes are the literary images that grow out of the "collective unconscious." They appear in literatureas incidents and plots that repeat basic patterns of life. They may also appear as stereotyped characters.Examples of literary archetypes include themes such as birth and death and characters such as the Earth Mother.Argument: The argument of a work is the author's subject matter or principal idea.Examples of defined "argument" portions of works include John Milton's Arguments to each of the books of Paradise Lost and the "Argument" to Robert Herrick's Hesperides.Aristotelian Criticism: Specifically, the method of evaluating and analyzing tragedy formulated by the Greek philosopher Aristotle in his Poetics.More generally, the term indicates any form of criticismthat follows Aristotle's views. Aristotelian criticism focuses on the form and logical structure of a work, apart from its historical or social context, in contrast to "Platonic Criticism," which stresses the usefulness of art. Adherents of New Criticism including John Crowe Ransom and Cleanth Brooks utilize and value the basic ideas of Aristotelian criticism for textual analysis. (Compare with Platonic Criticism.) (See also catharsis, New Criticism.)Art for Art's Sake: See Aestheticism.Aside: A comment made by a stage performer that is intended to be heard by the audiencebut supposedly not by other characters.Eugene O'Neill's Strange Interlude is an extended use of the aside in modern theater.Assonance: The repetition of similar vowel sounds in Poetry.The following lines from Gerald Manley Hopkins's "God's Grandeur" contain several patterns of assonance:The world is charged with the grandeur of God.It will flame out, like shining from shook foil;It gathers to a greatness, like the ooze of oilCrushed. Why do men then now not reck his rod?(Compare with Alliteration, Dissonance, and rhyme.)Audience: The people for whom a piece of literatureis written. Authors usually write with a certain audience in mind, for example, children, members of a religious or ethnic group, or colleagues in a professional field. The term "audience" also applies to the people who gather to see or hear any performance, including plays, Poetryreadings, speeches, and concerts.Jane Austen's parody of the gothic novel, Northanger Abbey, was originally intended for (and also pokes fun at) an audience of young and avid female gothic novel readers.Autobiography: A connected narrative in which an individual tells his or her life story.Examples include Benjamin Franklin's Autobiography and Henry Adams's The Education of Henry Adams.(Compare with Biography.) (See also Diaryand Memoirs.)Automatic Writing: Writing carried out without a preconceived plan in an effort to capture every random thought. Authors who engage in automatic writing typically do not revise their work, preferring instead to preserve the revealed truth and beauty of spontaneous expression.Automatic writing was employed by many of the Surrealist writers, notably the French poetRobert Desnos. (See also Surrealism.)Avant-garde: A French term meaning "vanguard." It is used in literary criticismto describe new writing that rejects traditional approaches to literaturein favor of innovations in style or content.Twentieth-century examples of the literary avant-gardeinclude the Black Mountain Schoolof poets, the Bloomsbury Group, and the Beat Movement.BBallad: A short poem that tells a simple story and has a repeated refrain. Ballads were originally intended to be sung. Early ballads, known as folk ballads, were passed down through generations, so their authors are often unknown. Later ballads composed by known authors are called literary ballads.An example of an anonymous folk ballad is "Edward," which dates from the Middle Ages. Samuel Taylor Coleridge's "The Rime of the Ancient Mariner" and John Keats's "La Belle Dame sans Merci" are examples of literary ballads. (Compare with Corrido and Oral Transmission.)Baroque: A term used in literary criticism to describe literature that is complex or ornate in style or diction. Baroque works typically express tension, anxiety, and violent emotion. The term "Baroque Age" designates a period in Western European literature beginning in the late sixteenth century and ending about one hundred years later. Works of this period often mirror the qualities of works more generally associated with the label "baroque" and sometimes feature elaborate conceits. Examples of Baroque works include John Lyly's Euphues: The Anatomy of Wit, Luisde Gongora's Soledads, and William Shakespeare's As You Like It.Baroque Age: See BaroqueBaroque Period: See BaroqueBeat Generation: See Beat MovementBeat Movement: A period featuring a group of American poets and novelists of the 1950s and 1960s — including Jack Kerouac, Allen Ginsberg, Gregory Corso, William S. Burroughs, and Lawrence Ferlinghetti —who rejected established social and literary values. Using such techniques as stream of consciousness writing and jazz-influenced free Verse and focusing on unusual or abnormal states of mind —generated by religious ecstasy or the use of drugs — the Beat writers aimed to create works that were unconventional in both form and subject matter.Kerouac's On the Roadis perhaps the best-known example of a Beat Generation novel, and Ginsberg's Howlis a famous collection of Beat Poetry.Beat Poets: See Beat MovementBeats, The: See Beat MovementBelles-lettres: A French term meaning "fine letters" or "beautiful writing." It is often used as a synonym for literature, typically referring to imaginative and artistic rather than scientific or expository writing. Current usage sometimes restricts the meaning to light or humorous writing and appreciative essays about literature.Lewis Carroll's Alice in Wonderland epitomizes the realm of belles-lettres.Bildungsroman: (Also known as Apprenticeship Novel, Coming of Age Novel, Erziehungsroman, or Kunstlerroman.) A German word meaning "novel of development." The bildungsromanis a study of the maturation of a youthful character, typically brought about through a series of social or sexual encounters that lead to self-awareness. Bildungsroman is used interchangeably with erziehungsroman,a novel of initiation and education. When a bildungsroman is concerned with the development of an artist (as in James Joyce's A Portrait of the Artist as a Young Man), it is often termed a kunstlerroman.Well-known bildungsromane include J. D. Salinger's The Catcher in the Rye, Robert Newton Peck's A Day No Pigs Would Die, and S. E. Hinton's The Outsiders. Biography: A connected narrative that tells a person's life story. Biographies typically aim to be objective and closely detailed. James Boswell's The Life of SamuelJohnson,LL.D is a famous example of the form. (Compare with Autobiography and Memoirs.Black Aesthetic Movement: (Also known as Black Arts Movement.) A period of artistic and literary development among African Americans in the 1960s and early 1970s. This was the first major African-American artistic movement since the Harlem Renaissance and was closely paralleled by the civil rights and black power movements. The black aesthetic writers attempted to produce works of art that would be meaningful to the black masses. Key figures in black aesthetics included one of its founders, poet and playwright Amiri Baraka, formerly known as LeRoi Jones; poet and essayist Haki R. Madhubuti, formerly Don L. Lee; poet and playwright Sonia Sanchez; and dramatist Ed Bullins.Works representative of the Black Aesthetic Movement include Amiri Baraka's play Dutchman, a 1964 Obie award-winner; Black Fire: An Anthology of Afro-American Writing,edited by Baraka and playwright Larry Neal and published in 1968; and Sonia Sanchez's poetry collection We a BaddDDD People, published in 1970.Black Arts Movement: See Black Aesthetic MovementBlack Comedy: See Black HumorBlack Humor: (Also known as Black Comedy.) Writing that places grotesque elements side by side with humorous ones in an attempt to shock the reader, forcing him or her to laugh at the horrifying reality of a disordered world.Joseph Heller's novel Catch-22 is considered a superb example of the use of black humor. Other well-known authors who use black humor include Kurt V onnegut, Edward Albee, Eugene Ionesco, and Harold Pinter.Black Mountain School: Black Mountain College and three of its instructors —Robert Creeley, Robert Duncan, and Charles Olson —were all influential in projective verse, so poets working in projective verse are now referred as members of the Black Mountain school.The Black Mountain Review published much of the work of Black Mountain school poets.Blank Verse: Loosely, any unrhymed poetry, but more generally, unrhymed iambic pentameter verse(composed of lines of five two-syllable feet with the first syllable accented, the second unaccented). Blank verse has been used by poets since the Renaissance for its flexibility and its graceful, dignified tone.John Milton's Paradise Lostis in blank verse, as are most of William Shakespeare's plays.(See also Accent, Foot, Measure, and Meter.)Bloomsbury Group: A group of English writers, artists, and intellectuals who heldinformal artistic and philosophical discussions in Bloomsbury, a district of London, from around 1907 to the early 1930s. The Bloomsbury Group held no uniform philosophical beliefs but did commonly express an aversion to moral prudery and a desire for greater social tolerance.At various times the circle included Virginia Woolf, E. M. Forster, Clive Bell, Lytton Strachey, and John Maynard Keynes.Bon Mot: A French term meaning "good word." A bon mot is a witty remark or clever observation.Charles Lamb and Oscar Wilde are celebrated for their witty bon mots. Two examples by Oscar Wilde stand out: (1) "All women become their mothers. That is their tragedy. No man does. That's his." (2) "A man cannot be too careful in the choice of his enemies."Breath Verse: See Projective VerseBurlesque: Any literary work that uses exaggeration to make its subject appear ridiculous, either by treating a trivial subject with profound seriousness or by treating a dignified subject frivolously. The word "burlesque" may also be used as an adjective, as in "burlesque show," to mean "striptease act."Examples of literary burlesque include the comedies of Aristophanes, Miguel de Cervantes's Don Quixote,, Samuel Butler's poem "Hudibras," and John Gay's play The Beggar's Opera.(Compare with Parody.)CCadence: The natural rhythm of language caused by the alternation of accented and unaccented syllables. Much modern Poetry—notably free Verse —deliberately manipulates cadence to create complex rhythmic effects.James Macpherson's "Ossian poems" are richly cadenced, as is the poetry of the Symbolists, Walt Whitman, and Amy Lowell. (Compare with Meter.)Caesura: A pause in a line of Poetry, usually occurring near the middle. It typically corresponds to a break in the natural rhythm or sense of the line but is sometimes shifted to create special meanings or rhythmic effects.The opening line of Edgar Allan Poe's "The Raven" contains a caesura following "dreary": "Once upon a midnight dreary, while I pondered weak and weary...." (Compare with Cadence.)Canzone: A short Italian or Provencal lyric poem, commonly about love and often set to music. The canzonehas no set form but typically contains five or six stanzas made up of seven to twenty lines of eleven syllables each. A shorter, five- to ten-line "envoy," or concluding stanza, completes the poem.Masters of the canzone form include Petrarch, Dante Alighieri, Torquato Tasso, andGuido Cavalcanti.Carpe Diem: A Latin term meaning "seize the day." This is a traditional theme of Poetry, especially lyrics. A carpe diem poem advises the reader or the person it addresses to live for today and enjoy the pleasures of the moment.Two celebrated carpe diem poems are Andrew Marvell's "To His Coy Mistress" and Robert Herrick's poem beginning "Gather ye rosebuds while ye may...."Catharsis: The release or purging of unwanted emotions — specifically fear and pity — brought about by exposure to art. The term was first used by the Greek philosopher Aristotle in his Poeticsto refer to the desired effect of tragedy on spectators.A famous example of catharsis is realized in Sophocles' Oedipus Rex,when Oedipus discovers that his wife, Jacosta, is his own mother and that the stranger he killed on the road was his own father. (See also Aristotelian Criticism.)Celtic Renaissance: (Also known as Celtic Twilight.) A period of Irish literary and cultural history at the end of the nineteenth century. Followers of the movement aimed to create a romantic vision of Celtic myth and legend. The most significant works of the Celtic Renaissance typically present a dreamy, unreal world, usually in reaction against the reality of contemporary problems.William Butler Yeats's The Wanderings of Oisinis among the most significant works of the Celtic Renaissance. (Compare with Irish Literary Renaissanceand romanticism.)Celtic Twilight: See Celtic RenaissanceCharacter: Broadly speaking, a person in a literary work. The actions of characters are what constitute the plot of a story, novel, or poem. There are numerous types of characters, ranging from simple, stereotypical figures to intricate, multifaceted ones. In the techniques of Anthropomorphismand personification, animals —and even places or things — can assume aspects of character. "Characterization" is the process by which an author creates vivid, believable characters in a work of art. This may be done in a variety of ways, including (1) direct description of the character by the narrator; (2) the direct presentation of the speech, thoughts, or actions of the character; and (3) the responses of other characters to the character. The term "character" also refers to a form originated by the ancient Greek writer Theophrastus that later became popular in the seventeenth and eighteenth centuries. It is a short essay or sketch of a person who prominently displays a specific attribute or quality, such as miserliness or ambition.Notable characters in literature include Oedipus Rex, Don Quixote de la Mancha, Macbeth, Candide, Hester Prynne, Ebenezer Scrooge, Huckleberry Finn, Jay Gatsby, Scarlett O'Hara, James Bond, and Kunta Kinte.Characterization: See CharacterChorus: In ancient Greek drama, a group of actors who commented on and interpreted the unfolding action on the stage. Initially the chorus was a major component of the presentation, but over time it became less significant, with its numbers reduced and its role eventually limited to commentary between Acts. By the sixteenth century the chorus — if employed at all — was typically a single person who provided a prologue and an epilogue and occasionally appeared between acts to introduce or underscore an important event.The chorus in William Shakespeare's Henry Vfunctions in this way. Modern dramas rarely feature a chorus, but T. S. Eliot's Murder in the Cathedral and Arthur Miller's A View from the Bridge are notable exceptions. The Stage Manager in Thornton Wilder's Our Town performs a role similar to that of the chorus.Chronicle: A record of events presented in chronological order. Although the scope and level of detail provided varies greatly among the chronicles surviving from ancient times, some, such as the Anglo-Saxon Chronicle,feature vivid descriptions and a lively recounting of events. During the Elizabethan Age, many dramas —appropriately called "chronicle plays" — were based on material from chronicles. Many of William Shakespeare's dramas of English history as well as Christopher Marlowe's Edward II are based in part on Raphael Holinshead's Chronicles of England, Scotland, and Ireland.Classical: In its strictest definition in literary criticism, classicism refers to works of ancient Greek or Roman literature. The term may also be used to describe a literary work of recognized importance (a "classic") from any time period or literature that exhibits the traits of classicism.Classical authors from ancient Greek and Roman times include Juvenal and Homer. Examples of later works and authors now described as classical include French literature of the seventeenth century, Western novels of the nineteenth century, and American fiction of the mid-nineteenth century such as that written by James Fenimore Cooper and Mark Twain.Classicism: A term used in literary criticism to describe critical doctrines that have their roots in ancient Greek and Roman literature, philosophy, and art. Works associated with classicism typically exhibit restraint on the part of the author, unity of design and purpose, clarity, simplicity, logical organization, and respect for tradition. Examples of literary classicism include Cicero's prose, the dramas of Pierre Corneille and Jean Racine, the Poetry of John Dryden and Alexander Pope, and the writings of J. W. von Goethe, G. E. Lessing, and T. S. Eliot.Climax: The turning point in a narrative, the moment when the conflict is at its most intense. Typically, the structure of stories, novels, and plays is one of rising action, in which tension builds to the climax, followed by falling action, in which tension lessens as the story moves to its conclusion.。
2020智慧树知道网课《英语学术论文写作》课后章节测试满分答案

第一章测试1【判断题】(5分) Peopledoresearchinordertohaveabetterunderstandingofourworld.A.对B.错2【判断题】(5分)Wedon’tneedtobecreativeinresearchasanyresearchisbasedonevidences.A.错B.对3【判断题】(5分)Goodresearchquestionsareimportantinresearch.A.错B.对4【判断题】(5分) Literaturereviewisneededinsomeresearchpapers.A.对B.错5【判断题】(5分) Agoodresearchpaperneedscarefulrevisingandproofreading.A.错B.对6【判断题】(5分) Unintentionalplagiarismcanbeexcusedasitisnotcommitteddeliberately.A.对B.错7【判断题】(5分) Academicfalsificationisonecommontypeofacademicdishonesty.A.对B.错8【多选题】(5分) Whatroledoesaliteraturereviewplayinaresearchpaper?A.Itenhancesthecredibilityofyourpaper.B.Itprovestheexistenceofaresearchgap.C.Itsynthesizestheexistingstudiesinyourarea.D.Itprovidesevidencesforyourargument.9【单选题】(5分) WhichofthefollowingisNOTanessentialstepinaresearchpaperwriting?A.ChoosingatopicB.ConsultinginstructorsC.LocatingsourcesD.OutliningthePaper10【多选题】(5分) Whichofthefollowingmayleadtoacademicdishonesty?A.AcademicplagiarismB.AcademicpromotionC.AcademicinterestD.Academicfabrication第二章测试1【多选题】(5分)Inchoosingaresearchtopic,whichofthefollowingdoweneedtoconsider?A.PublicationpossibilitiesB.AcademicimportanceC.OurresearchinterestD.Ourmanageability2【判断题】(5分)Tocheckthevalidityofaresearchtopic,weneedtobecriticalenough.A.对B.错3【判断题】(5分) Itisanactofplagiarismifonesimplyparaphrasesabookforaresearchpaper.A.对B.错4【判断题】(5分) StudentsshouldbebraveenoughtotrychallengingissuesfortheirBAtheses.A.对B.错5【判断题】(5分) Carefulreadingofliterarytextisimportantinliterarystudies.A.对B.错6【判断题】(5分) Literarytheoriestakepriorityoverliterarytextsinliteraryanalysis.A.错B.对7【判断题】(5分) ICstudycanbechallengingbutinterestingandpracticalinlife.A.错B.对8【判断题】(5分) Expertssharesimilarunderstandingsoverthestandardsoftranslation.A.错B.对9【判断题】(5分) Translationstrategiesandtranslationtechniquesaredifferentconceptsintranslation.A.对B.错10【判断题】(5分) Sociolinguisticsisoneofthebranchesofappliedlinguistics.A.对B.错第三章测试1【判断题】(5分) Aworkingbibliographyincludesthesourceswehavesofarcollectedforaresearchproject.A.对B.错2【判断题】(5分) Knowingwhatsourcesyouneedisofvitalimportanceinevaluatingyoursources.A.错B.对3【判断题】(5分) Sourcesfromleadingscholarscanbeveryusefultoyourresearch.A.错B.对4【判断题】(5分)Inreadingsources,weneedtobecriticalandformourpersonalresponses.A.对B.错5【判断题】(5分) Theplanforanempiricalresearchshouldcoveritspurpose,method,subjects,andprocedure.A.错B.对6【判断题】(5分) Itiswisefortheobservernottotakepartintheactivityobservedatalltime.A.错B.对7【多选题】(5分)InBooleanLogic,ifonewantstosearchonlyforsourcesrelevantwithcomputervirus,thesearchformul ashouldbe_______.A.computernotvirusB.computernearvirusC.computerorvirusD.computerandvirus8【多选题】(5分) Theannotationofasourceinanannotatedbibliographymayhaveyour____:A.reflectionofthesourceB.adaptationofthesourceC.summaryofthesourceD.assessmentofthesource9【单选题】(5分)Inaquestionnaireentitled“ASurveyontheThirdYearEnglishMajors’EnglishVocabularyinXXUniv ersity”,the“thethirdyearEnglishmajors”shouldbe:A.ThetimeofthesurveyB.ThesubjectofthesurveyC.ThecontentofthesurveyD.Themodeofthesurvey10【单选题】(5分)Inanexperimententitled“AStudyontheEffectsofWriting-after-ReadingActivityonEnglishMajors EnglishVocabulary”,students’languageproficiencyshouldbe:A.IrrelevantvariableB.dependentvariableC.Independentvariable第四章测试1【判断题】(5分)Aresearchproposalshouldbepersuasiveinnature.A.错B.对2【判断题】(5分) Wecanaskforsuggestionsfromtheexpertsforourresearchinourproposal.A.错B.对3【判断题】(5分) Weneedtoputforwardourresearchquestionsinourresearchproposals.A.错B.对4【判断题】(5分) Aresearchproposaldemonstratesthesignificanceofourproposedresearch.A.错B.对5【判断题】(5分) Theliteraturereviewsectioninaproposalprovestheexistenceofaresearchgap.A.对B.错6【判断题】(5分)The“ApplicationoftheResearch”tellshowyourresearchwillbenefitinpractice.A.对B.错7【判断题】(5分)A“PreliminaryBibliography”isalistofthesourcesyouhavecitedinyourproposal.A.错B.对8【判断题】(5分) Thesignificanceofyourresearchemphasizespossibleresearchcontributions.A.对B.错9【判断题】(5分)Simpleasitis,aBAthesisproposalincludesalltheelementsinagrantresearchproposal.A.错B.对10【多选题】(5分)WhichofthefollowingisNOTincludedinthree-moveschemeoftheproposalsummary?A.researchneedB.potentialcontributionsC.possiblelimitationsD.researchmethod第五章测试1【判断题】(5分) Theuseofsignalphrasesincitationcanenhancethefluencyofwriting.A.对B.错2【判断题】(5分) Theintegrationofthesourcesmustfitourwritinginstructureandgrammar.A.错B.对3【判断题】(5分) Researchlimitationisacompulsoryelementinallresearchpapers.A.错B.对4【判断题】(5分) CARSModelisapatternforintroductionwritinginresearchpapers.A.对B.错5【判断题】(5分)Inrevising,weneedtofocusontheerrorslikegrammarandspelling.A.错B.对6【判断题】(5分) Aliteraturereviewmainlypresentsasummaryofeachsourceinchronologicalorder.A.错B.对7【单选题】(5分) Characteristicsofacademicwritingincludesallthefollowingexcept________.A.thefirst-personviewB.aformaltoneC.aclearfocusD.precisewordchoice8【单选题】(5分) Itisessentialtoalwaysacknowledgethesourceofborrowedideasinyourpaper.Todootherwiseisconsi dered_________.A.IgnoranceB.CarelessnessC.Plagiarism9【单选题】(5分)Whichofthefollowingarefeaturesofagoodtitle?A.ClearB.AlloftheaboveC.Attractive.D.Direct10【单选题】(5分)Abstractscanbedividedintotwotypes accordingto their_________.A.functionsB.length第六章测试1【判断题】(5分)MLAin-textcitationrequiresthattheauthorinformationshouldbeputeitherinthetextofthepaperorint heparentheticalcitation.A.错B.对2【判断题】(5分)InMLAdocumentation,parentheticalcitationinthetextofthepapermustalwaysincludetheauthor’sn ameandthework’stitle.A.错B.对3【判断题】(5分)MLAworkscitedlistisorganizedalphabeticallybytheauthor’slastnames(orbytitleforaworkwithnoa uthor).A.错B.对4【判断题】(5分)Whenawork’sauthorisunknown,theworkislistedunder“Anonymous”inthelistofMLAworkscited.A.错B.对5【单选题】(5分)Lee(2007)stated,“Theabilitytothinkcriticallyisneededinthisrevolutionaryageoftechnologicalcha nge”(p.82).Thein-textcitationusedhereis__________.A.integratedB.non-integrated6【多选题】(5分)WhatarethefunctionsofAPAdocumentation?A.Tociteothers’ideasandinformationusedwithinyourpaperB.TodemonstratethetypeofresearchconductedC.ToindicatethesourcesintheReferenceslist7【单选题】(5分)Writethelistofworkscited,usingtheAPAformat.Aparaphraseofanideafrompage121ofWritingSpace:TheComputer;Hypertext,andtheHistoryofWr iting,byJayDavidBolter.This1991bookwaspublishedbyLawrenceErlbaumAssociatesofHillsdale, NewJersey.Whichofthefollowingiscorrectfortheauthor’sname?A.Bolter,J.DB.J.D.BolterC.Jay,D.BolterD.Bolter,JayDavid【单选题】(5分)Writethelistofworkscited,usingtheAPAformat.Aquotationfrompage78ofanarticlebyBartKoskoandSatornIsakafromtheJuly1993issueofScientifi cAmerican,amonthlypublication.Thearticleisentitled"FuzzyLogic"andappearsonpages76to81in volume239,ofthejournal. Whichofthefollowingisthecorrectformatforthevolumeandpagernumber?A.ScientificAmerican,239,78B.ScientificAmerican,239,76-81C.ScientificAmerican,239:76-81D.ScientificAmerican,239,76-81.9【多选题】(5分)Bohren,M.A.,G.J.Hofmeyr,C.Sakala,R.K.Fukuzawa,andA.Cuthbert.(2017).Continuoussupportf orwomenduringchildbirth.CochraneDatabaseofSystematicReviews,2017(7).https:///10.1 002/14651858.CD003766.pub6Errorsmadeinthisentryinclude_________.A.authors’namesB.issuenumberC.theword“and”D.doinumber10【单选题】(5分)Perrey,S.(2017).Doweperformbetterwhenweincreaseredbloodcells?TheLancetHaematology,17, 2352-3026.https:///10.1016/S2352-3026(17)30123-0.RetrievedAugust23,2018.Whatinfo rmationisNOTneededinthisentry?A.ThedateofretrievalB.ThepagerangeC.Thedoinumber。
交互英语第一单元

Preview and preparation of listening materials
Previewing the content
01
Skimming through the material to get a general
idea of the topic and the context
Interactive English is a comprehensive English course that focuses on developing students' communicative competence
It emphasizes on practical language use and resources students to actively participate in classroom interactions
Theme: Introduction to English Language and Culture
Content: This unit introduces students to the basics of English language and culture, including
greetings, introductions, and common everyday expressions
01
02
03
Word 1
Definition
and
example presence
Word 2
Definition
and
example presence
Word 3
Definition
and
example presence
新视野第二册课后段落翻译(配中文)

Unit 1中国书法(calligraphy)是一门独特的艺术,是世界上独一无二的艺术瑰宝。
Chinese calligraphy is a unique art and the unique art treasure in the world.中国书法艺术的形成、发展与汉文字的产生与演进存在着密不可分的关系。
The formation and development of the Chinese calligraphy is closely related to the emergence and evolution of Chinese characters.汉字在漫长的演变发展过程中,一方面起着交流思想、继承文化的重要作用,另一方面它本身又形成了一种独特的艺术。
In this long evolutionary process, Chinese characters have not only played an important role in exchanging ideas and transmitting culture but also developed into a unique art form.书法能够通过作品把书法家个人的生活感受、学识、修养、个性等折射出来,所以,通常有"字如其人"的说法。
Calligraphic works well reflect calligraphers' personal feelings, knowledge, self-cultivation, personality, and so forth, thus there is an expression that "seeing the calligrapher's handwriting is like seeing the person".中国书法不仅是中华民族的文化瑰宝,而且在世界文化艺术宝库中独放异彩。
调查问卷报告_英语模板

IntroductionThis report presents the findings from a survey conducted to [briefly describe the purpose of the survey]. The survey was designed to gather insights on [topic/issue being investigated], aiming to understand the opinions, behaviors, and preferences of [target audience]. The following sections provide a detailed analysis of the survey results, includingthe methodology, key findings, and recommendations.Methodology1. Survey Design- The survey was conducted [describe the type of survey: online, paper-based, etc.].- It consisted of [number] questions, which were categorized into [list question types: multiple-choice, open-ended, Likert scale, etc.].2. Sample- The survey was distributed to [number] participants.- The sample was [describe the sampling method: random, stratified, convenience, etc.].- The participants were [describe the target audience: age range, gender, occupation, etc.].3. Data Collection- The survey was [describe the data collection period: e.g., from [start date] to [end date]].- Responses were collected through [describe the platform or method:e.g., email, online survey tool, etc.].4. Data Analysis- Data were analyzed using [mention the statistical software or methods used: e.g., SPSS, Excel, descriptive statistics, etc.].- Responses were coded and categorized to ensure consistency and accuracy.Key Findings1. Overall Satisfaction- [Percentage] of respondents reported being [satisfied/unsatisfied] with [the topic/issue being investigated].- The most common reasons for satisfaction/unatisfaction were [list reasons based on survey results].2. Frequency of Occurrence- [Percentage] of participants indicated that [the topic/issue] occurs [frequently/infrequently] in their daily lives.- Factors contributing to the frequency were [list factors based on survey results].3. Preferences and Choices- When asked about [specific preference or choice], [percentage] of respondents preferred [option A], [percentage] preferred [option B], and [percentage] preferred [option C].- The reasons for these preferences were [elaborate on the reasons based on survey results].4. Open-Ended Questions- Responses to open-ended questions provided valuable insights into [topic/issue]:- [Summary of responses 1]- [Summary of responses 2]- [Summary of responses 3]5. Demographic Analysis- The survey revealed that [describe any significant demographic patterns or trends]:- [Example: Older age groups were more likely to express satisfaction with the current system, while younger respondents preferred more innovative solutions.]ConclusionThe survey results provide valuable insights into the opinions and behaviors of [target audience] regarding [topic/issue]. The findings suggest that [state a general conclusion or trend]. However, it is important to note that [mention any limitations of the survey or areas that require further investigation].RecommendationsBased on the survey findings, the following recommendations are proposed:1. [Recommendation 1]2. [Recommendation 2]3. [Recommendation 3]Additional Considerations- Further research is needed to [mention any areas that require more in-depth analysis].- The findings of this survey should be considered in conjunction with other data sources to provide a comprehensive understanding of the issue.References[If applicable, include a list of references or sources used in the survey or report.]---This template can be adapted to fit the specific details of your survey. Remember to include relevant data and analysis specific to your survey's objectives and target audience.。
新视野大学英语第三版第一册Unit Section A

Lead-in Pre-reading activities
Lead-in
Survey: How close are you and your parents?
Check (√) the statements which are true for you.
Main idea & structure
Main ideas of the parts
Disagreements between the mother and the daughter (Paras.1-3):
Daughter Tips
Mother Tips
• a truck, large & expensive;
I have been missing my parents a lot since I left for college.
I often chat with my parents. I like sharing my joys and sorrows with my parents. I always remember my parents’ birthdays. I know about my parents’ hobbies. My parents allow me to make my own decisions.
Pre-reading activities
Watch and Talk
Watch a video clip and answer the following questions.
1. What happened to the father and the son?
2. Is there any conflict or disagreement between you and your parents? And what should we do to settle the conflict or disagreement?
外文文献翻译——顾客满意度(附原文)

外文文献翻译(附原文)译文一:韩国网上购物者满意度的决定因素摘要这篇文章的目的是确定可能导致韩国各地网上商场顾客满意的因素。
假设客户的积极认知互联网购物的有用性,安全,技术能力,客户支持和商场接口积极影响客户满意度。
这也是推测,满意的顾客成为忠实的客户。
调查结果证实,客户满意度对顾客的忠诚度有显著影响,这表明,当顾客满意服务时会显示出很高的忠诚度.我们还发现,“网上客户有关安全风险的感知交易中,客户支持,网上购物和商场接口与客户满意度呈正相关.概念模型网上购物者可以很容易的将一个商场内的商品通过价格或质量进行排序,并且可以在不同的商场之间比较相同的产品。
网上购物也可以节省时间和降低信息搜索成本。
因此,客户可能有一种感知,他们可以用更少的时间和精力得到更好的网上交易。
这个创新的系统特性已被定义为知觉有用性。
若干实证研究发现,客户感知的实用性在采用影响满意度的创新技术后得以实现.因此,假设网上购物的知觉有用性与满意度成正相关(H1).网上客户首要关注的是涉及关于网上信用卡使用的明显的不安全感。
虽然认证系统有明显进步,但是顾客担心在网上传输信用卡号码这些敏感的信息是不会被轻易的解决的。
网上的隐私保护环境是另一个值得关注的问题。
研究表明,网上客户担心通过这些网上业务会造成身份盗窃或冒用他们的私人信息。
因此,据推测,网上购物的安全性对顾客满意度有积极地影响(H2)。
以往的研究表明,系统方面的技术,如网络速度,错误恢复能力和系统稳定性都是导致客户满意度的重要因素。
例如,Kim和Lim(2001)发现,网络速度与网上购物者的满意度有关.Dellaert和卡恩(1999年)也报告说,当网络提供商没有进行很好的管理时网上冲浪速度慢会给评价网站内容带来负面影响。
丹尼尔和Aladwani的文件表明,系统错误的迅速准确的恢复能力以及网络速度是影响网上银行用户满意度的重要因素(H3)。
由于网上交易的非个人化性质客户查询产品和其他服务的迅速反应对客户满意度来说很重要。
cochrane纳入的RCT文献质量评价风险偏倚评价工具中英文对照版

分配隐藏
分配前不充足的分配隐藏导致选择偏倚
低风险判断标准
参与者以及纳入参与者的研究者因以下掩盖分配的方法 或相当的方法,事先不了解分配情况
•中心分配(包括电话,网络,药房控制随机)
•相同外形的顺序编号的药物容器;
•顺序编号、不透明、密封的信封
高风险判断标准
参与者以及纳入参与者的研究者可能事先知道分配,因
而引入选择偏倚,譬如基于如下方法的分配:
•使用摊开的随机分配表(如随机序列清单)
•分发信封但没有合适的安全保障(如透明、非密 封、非顺序编号)
•交替或循环
•出生日期
•病历号
•其它明确的非隐藏过程
风险未知
没有足够信息判断为低风险或高风险。通常因分配隐藏 的方法未描述或描述不充分。例如描述为使用信封分配, 但为描述信封是否透明?密封?顺序编号?
outcome assessors.
Attrition bias.
Incomplete outcome dataAssessments should be made for each main outcome (or class of outcomes).?
Describe the completeness of outcome data for each main outcome, including attrition and exclusions from the analysis. State whether attrition and exclusions were reported, the numbers in each intervention group (compared with total randomized participants), reasons for attrition/exclusions where reported, and any re-inclusions in analyses performed by the review authors.
1998 Wharton Survey of Financial Risk Management by U.S. Non-Financial Firms

GEORGE WEISS CENTERFOR INTERNATIONAL FINANCIAL RESEARCH WHARTON SCHOOL, UNIVERSITY OF PENNSYLVANIAandCIBC WORLD MARKETS1998 Survey of Financial Risk Management by U.S. Non-Financial Firmswritten byGordon M. Bodnar and Richard C. Marston,Wharton School, University of PennsylvaniaandGreg Hayt, CIBC World MarketsJuly 1998WHARTON/CIBC WORLD MARKETS 1998 SURVEY OF FINANCIAL RISK MANAGEMENT BY U.S. NON-FINANCIAL FIRMSExecutive SummaryThis is the third in a series of surveys on financial risk management practice and derivatives use by non-financial corporations in the United States undertaken by the Weiss Center for International Financial Research of the Wharton School. This 1998 survey, written in partnership again with CIBC World Markets, extends the previous two surveys by asking new questions about certain aspects of derivative use and risk management practice. Among the key findings are the following:(1) There is no evidence that the number of firms using derivatives has declined overtime (as a result of well-publicized derivative losses at Proctor & Gamble and other firms).Among firms participating in one or more of our previous surveys, the proportion using derivatives is unchanged. Among firms using derivatives, 42% report that their usage has actually increased as opposed to 13% who report that their usage has decreased.(2) As in past surveys, the use of derivatives is much higher among large firms (83%)than among small firms (12%), and higher among primary product firms (68%), and manufacturers (48%) than among firms in the service industry (42%). But, the intensity of use of derivatives by service firms is increasing significantly faster than for other firms.(3) A surprisingly large percentage of firms (73%) report that FASB’s new rulegoverning derivatives activity will have no effect on their derivative use or risk management strategy.(4) A new question about revenue and expense exposure in foreign currency reveals awide range of exposure across firms. For example, 40% of the firms with foreign exchange exposure report that their revenues in foreign currency are 20% or more of total revenues, and almost as large a percentage report foreign currency expenses to be 20% or more of their total expenses. On the other hand, 60% of the firms report that they have a balance between total foreign currency revenues and expenses (though this may not be true for revenues and expenses in each currency).(5) Among firms with significant foreign exchange exposure that regularly hedge, thereseems to be a general tendency to hedge only a small fraction of the total foreign currency exposure of the firm. Even in the case of payables and receivables, for example, the average firm hedges less than 50% of the perceived exposure.The majority of hedges, moreover, are short-dated. 82% of firms use foreign currency derivatives with a maturity of 90 days or less and for 40% of firms, these account for the majority of their foreign currency derivative use.(6) A wider variety of options have become common in the marketplace in the last few years, but firms continue to use standard European-style or American-style options much more than such exotic options as average rate, basket, or barrier options.(7) When asked which best describes how the risk management function is evaluated, 40% of the firms chose increased profit relative to a benchmark or absolute profit rather than reduced volatility (40%) or risk adjusted performance (21%). In addition, 32 % of firms that use derivatives reported that their market view of exchange rates leads them to “actively take positions” at least occasionally. A similar result is found for the market view of interest rates.(8) Most firms seem to have internal controls over derivative use. A large percentage of respondents, 86%, have either a written policy about the use of derivatives or a regular schedule for reporting derivative use to the firm’s board of directors.(9) Firms are concerned about counterparty risk, especially when longer-dated derivatives are concerned. 40% of the firms using derivatives insist on a credit rating of AA or better for their counterparty, an insistence that precludes derivative transactions with many U.S. banks.WHARTON/CIBC WORLD MARKETS 1998 SURVEY OF FINANCIAL RISK MANAGEMENT BY U.S. NON-FINANCIAL FIRMSI.INTRODUCTIONThis is the third in a series of surveys on financial risk management practice and derivatives use by non-financial corporations in the United States undertaken by the Weiss Center for International Financial Research of the Wharton School and CIBC World Markets. The results of the two earlier surveys, carried out in 1994 and 1995, are published in Financial Management and have been widely cited in the business and academic press.1 The 1998 survey, once again sponsored by CIBC World Markets, extends the previous two surveys by asking new questions about certain aspects of derivatives use and risk management practice. This report provides a summary of the responses to the questions on the 1998 survey, both in total and, when responses differ, conditional on size and industrial sector. Also, where appropriate, current responses are compared with responses to similar questions on previous surveys. However, caution is required in interpreting some of those comparisons as some differences may result simply from changes in the set of responding firms. A copy of the questionnaire with the response tallies is displayed in the Appendix.As with the previous surveys, one of the primary objectives of this survey is the development of a database on risk management practices suitable for academic research. The survey results can be linked with industry and firm-specific characteristics of the respondents to allow economic analysis of the responses. However, as in the past, the firm-specific responses are confidential and known only to the researchers at Wharton.E OF DERIVATIVESA. Sample Firms and Overall Derivative UsageThe six-page questionnaire was mailed in October 1997 to the same basic sample of firms used in the 1994 and 1995 surveys. The sample consists of the original randomly selected 2000 publicly traded firms used in 1994 plus the remaining 154 non-financial Fortune 500 firms added in 1995. Due to mergers, buyouts, and bankruptcies since 1994, this sample currently consists of 1928 firms. A second mailing of the questionnaire was done in March 1998. 399 firms returned a completed survey, yielding a response rate of 20.7%. Of these firms, 197 are from the manufacturing sector, 82 are from the primary products sector, which includes agriculture, mining, and energy, as well as utilities, and 120 are from the service sector. In terms of size, 160 firms are from the large category, consisting of firms with fiscal year 1996 total sales greater than $1.2 billion, 116 are from the medium-sized category, with total sales between $1.2b and $150m and 123 are from the small category, with total sales less than $150m.21The report on the 1994 survey can be found in Financial Management 24, Fall, 1995, and the report on the 1995 survey can be found in Financial Management 25, Winter, 1996. These results of these studies have been cited in Barrons, Business Week, Financial Times, The Economist, Forbes, and the Washington Post, among others.2 These size groups were defined based upon cutoff points that divided the entire sample of 1928 firms into three equal sized groups. Given our response total, equivalent response rates across size groups would imply 133 responses per group.The first question in the survey asks firms whether they use derivatives. 200of the firms, 50%, report usingderivatives. Table 1 displays the breakdown of the first question. In the “Full Sample” row of Table 1, we compare this usage rate with that of the previous two surveys. The resultssuggest that the percentage of responding firms using derivatives has increased each year. However, this increase over time may be a combination of the change in the sample in 1995and/or variation in response composition.A better way to compare derivatives use over time is to compare the response of the same set of firms. In the second rowof Table 1, we report the usagepercentages for the 58 firms that responded to all three surveys. Interestingly, in all three years the percentage of derivative users from this group is 41%, although several firms switch between use and non-use across years. Because of the limited number of firms that responded to all three surveys, we also report the usage percentages for the 171 firms that responded to both the 1994and the 1998 surveys. These percentages, reported in the bottom row of Table 1, are also the same in both years at 44%. Overall, these results suggest that the percentage of firms using derivatives has remained constant over the past three years.B. Change in Usage IntensityWhile the evidence suggests that the percentage of firms usingderivatives has not changednoticeably, we were interested in determining whether there was anychange in the intensity of usageamong the firms that usederivatives. To consider this,Question 2 asks the derivative using firms to indicate how theirderivative usage in the current yearcompared to usage in the previous year (based upon the notional value of total contracts). Figure 1displays the response to this question. 42% of derivative usersindicated a decrease. The remaining firms indicated that their usage had remained constant.Table 1: Comparison of Derivative Usage Across Surveys Year of Survey Percentage of Respondents using Derivatives This Survey 199850%Previous Years’ Surveys 1995199441%35%Firms responding to all three surveys (58 firms)19981995199441%41%41%Firms responding in both1994 & 1998 (171 firms)1998199444%44%Overall, these responses suggest that a significant proportion of derivative users is finding derivatives helpful enough that they are choosing to increase their usage.C. Derivative Usage Conditional on Size and ActivityFigure 2 presents the percentage of current derivative users broken down by size group and industrial sector. In the size dimension, usage is heaviest among large firms at 83%. The derivative usage rate drops to 45% for medium-sized firms and to 12% for small firms. That large firms are so much more likely to use derivatives is suggestive of an economies-to-scale argument for derivative use, with large firms better able to bear the fixed cost of derivatives use compared to small firms. In the industrial dimension, derivatives usage is greatest among primary product producers at 68%. Given that futures exchanges were originally established to help manage commodity risks, it is not surprising that such a large percentage of primary product producers use derivatives. Among manufacturing firms 48% use derivatives, much of this likely driven by foreign currency exposure arising from foreign operations or exporting/importing. But even among service firms, 42% use derivatives, most likely because of the increased internationalization of service firms and the growing need to manage foreign currency exposure.The change in derivative usage also varies across these groupings. Service firms are nearlytwice as likely to have increased derivative usage than manufacturing or primary product firms. Also, not a single small firm indicated that it had decreased its derivative usage over the previous year. These responses suggest that the usage rate is increasing most among groups where overall derivative usage is least common.D. Approach to Risk Management Across Risk ClassesFinancial price risk can be classified into four broad classes: foreign currency, interest rate, commodity and equity risk. We were interested in the percentage of firms that used derivatives to manage risk in each of these four classes. The responses to this question are displayed as the white bars in Figure 3. The figure reveals that of the firms using derivatives, foreign exchange (FX) is the risk most commonly managed with derivatives, being done so by 83% of all derivative users. Interest rate (IR) risk is the next most commonly managed risk with 76% of firms indicating IR derivatives use. Commodity (CM) risk is managed with derivatives by 56% of derivative users, while equity (EQ) risk is the least commonly managed risk at just 34%.3 It should be noted that unlike EQ risk and IR risk, which are likely to be faced by all firms, some firms will not directly face FX and CM risk because of the nature of their activities. Consequently, the usage of derivatives in these classes, conditional on having an exposure, will be even higher than the numbers displayed in the figure.5%12% The responses to this question conditional on industry display an interesting pattern. Among primary product firms, commodity risk is the most commonly managed risk with 79% of these firms indicating CM derivative use. FX risk is most commonly managed by manufacturing firms with 95% of this group indicating FX derivative use. For service firms, IR risk was slightly more commonly managed with derivatives than FX risk, with derivative usage rates of 78% versus 72%, respectively. Equity risk was managed least frequently by service firms with only 22% indicating EQ derivative use.3 Examples of equity risks that are commonly hedged with equity derivatives by non financial firms include using equity puts as part of a share repurchase program, or using total return swaps to monetize equity positions in other companies.Because of the different nature of these risk classes and the fact that they are often managed separately within firms, we also asked the firms to indicate their approach, in terms of decision-making structure, to managing each class of risk. We allowed firms to choose between i) risk management activities being primarily centralized, ii) risk management decisions primarily decentralized with centralized coordination, or iii) risk management activities primarily decentralized. The responses to this inquiry are also shown in Figure 3 as the multicolored bars under each risk class. As can be seen, centralized risk management activities are overwhelmingly most common, with the only exception being commodity risk management where one-third of firms indicated some degree of decentralized structure.E. Concerns about Derivatives UsageThe use of derivatives in today’s market involves many issues. Question 4a asks respondents to indicate their degree of concern about a series of issues regarding the use of derivatives. These issues include: accounting treatment, credit risk, market risk (unexpected changes in price of derivatives), monitoring and evaluating hedge results, reaction by analysts and investors, SEC disclosure requirements, and secondary market liquidity (ability to unwind transactions). For each issue, firms are asked to indicate a high, moderate, or low level of concern or indicate that the issue is not a concern to them. Firms were also given the option of listing any other issues of high concern to them regarding derivative use. Figure 4 displays the responses. Given the propensity of a majority of firms to indicate a moderate concern with many issues, the figure displays the percentage of firms indicating a high or low degree of concern for the six issues.37% of firms indicating a high concern and only 15% low or no concern with this issue. Undoubtedly, this is the result of the August 1997 release by the FASB of a draft proposal for anew accounting standard for the measurement and reporting of derivatives. Market risk, defined as unforeseen changes in the market value of derivative positions was the next issue most troubling firms, with 31% of firms indicating a high degree of concern and 34% of firms indicating little or no concern. This was followed closely by monitoring and evaluating hedge results with 29% of firms indicating a high degree of concern but 29% indicating little or no concern. The remaining four issues all had significantly more firms indicating little or no concern compared to high concern. In the case of credit risk, secondary market liquidity, and reaction by analysts and investors, more than 40% of firms indicated low or no concern with these issues. For credit risk, this result contrasts markedly with the 1995 survey in which it was the issue causing the most concern among derivative users. Among the “other issues” that some firms indicated high concern about were transaction costs and unauthorized trading.We also asked firms to indicate their most serious concern from the items listed above. The percentage of firms indicating each concern as their most serious are displayed on the right hand edge of Figure 4. Interestingly, market risk came in first with 27% of firms indicating this as their most serious concern. This was followed closely by accounting treatment with 26% of firms ranking as their most serious concern. Despite the large percentage of firms indicating little or no concern about credit risk, 14% ranked this as their most serious concern just ahead of monitoring and evaluating hedges which was ranked the most serious concern by 13% of firms.F. Likely Impact of FASB’s New Accounting RulesGiven the degree of concern regarding the accounting treatment of derivatives, we were interested in investigating the potential impact of the FASB’s new accounting standard, Statement No. 133, "Accounting for Derivative Instruments and Hedging Activities." This new standard, originally released in draft form in June 1996, then modified and re-released in August 1997, and then formally issued in June 1998, requires some significant changes to the way derivatives are measured and reported in the firm’s financial statement. It also provides official recognition to the use of a broader array of derivative for use in hedging transactions. Roughly speaking, the new proposal requires all derivatives be recorded on the balance sheet at fair market value and marked to market each reporting period. Changes in market value are either reported in income each period, or directly in the equity section of the balance sheet, depending on the specific use of the derivatives. The rule also essentially covers all derivative instruments, including derivatives embedded in other securities, thus expanding the set of derivatives instruments for which accounting rules are explicitly stated.In Question 5, we asked firms to indicate the most likely impact on their risk management activities of the FASB’s new rule on derivatives accounting. Table 2 displays the results. For 73% of firms, the new rules will have no effect on their derivative use or their risk management strategies. Of the 27% of firms for whom the new rules will cause some change, the most likely effect is a change in the type of instruments used, with 55% of these firms indicating this change. Other commonly chosen effects include a reduction in the use of derivatives and a change in the timing of hedging transactions.Table 2: Impacts of FASB’s New Rule Governing Derivatives AccountingMost Likely Impact:Percentage of Firms Responding*No effect on derivative use or risk management strategy73%Firms FindingFASB effects A reduction in the use of derivatives38%An increase in the use of derivatives9%A change in the types of instruments used55%A change in the timing of hedging transactions38%A significant change in the firm’s overall approach to risk management13%* Percentages in right column are with respect to those firms not agreeing with the first statement.III.FOREIGN EXCHANGE EXPOSURE MANAGEMENTA. Currency ExposureThe next section of questions addresses the issue of currency exposure and its management using derivatives. As shown above, foreign currency derivatives are the most commonly used class of derivatives with 83% of derivative-using firms utilizing them. Before asking detailed questions on foreign currency derivative use, we were interested in learning about the exposure of the sample firms. To do this, Question 7 asks firms to indicate their percentage of total revenues and costs in foreign currency. The responses to this question for revenues and expenses as well as net foreign currency position are displayed in Table 3.As the table shows, a reasonable percentage of firms report either no foreign currency revenue or no foreign currency costs. On the other hand, 40% of firms report foreign currency revenues to be 20% or more of total revenues, while 36% of firms report foreign currency expenses to be 20% or more of total expenses. So there are many firms in the survey that have significant foreign currency exposure.Table 3: Foreign Currency Revenues and ExpensesAmong Derivatives Users% of Firms Responding in Each Category % of total Revenues Expenses Imbalance -508%0%-405%1%-3011%1%-256%0%-206%1%-157%2%-1012%4%-520%6%028%25%60%514%9%1011%6%156%2%206%5%256%3%309%1%407%0%50+12%0% Table displays the frequency distribution of firm responses to the question: What percentage of your total revenues/expenses is denominated in foreign currency. Expenses are displayed as negative values. Third column is the imbalance of FC revenues and expenses for firms reporting both revenues and expense results.The fourth column of the table displays the net imbalance of foreign currency revenues and expenses for the firms. It is interesting that a majority of firms roughly balance out total foreign currency revenues with foreign currency expenses. Although the responses mask whether the expenses and revenues are in the same foreign currencies, and thus many of these balanced firms may have exposures to particular foreign currencies, this pattern suggests that natural hedging is a common way for firms to manage their exposure to exchange rates. Of the firms that report a net imbalance in total foreign currency revenues and costs, there are nearly twice as many firms with a net revenue exposure (26%) than a net expense exposure (15%).Conditionally, these revenue and expense exposures exhibit several interesting characteristics. First of all, large and medium firms are both substantially net-revenue exposed, while the small firms are, on average, net-expense exposed. Across industries, manufacturing and service firms are heavily revenue exposed with more than three times as many net-revenue exposed firms as net-expense exposed firms. This is offset by a heavy net-expense exposure on the part of the primary product firms.B. Transactions In Foreign Currency Derivative MarketsAs in the previous two surveys, firms were asked to indicate how often they transacted in the foreign currency derivatives market for hedging eight frequently cited exposures. These were contractual commitments--both on-balance sheet (i.e., payables and receivables) and off-balance sheet (i.e., signed contracts pending), anticipated transactions within one year, anticipated transactions beyond one year, economic/competitive exposure, translation of foreign accounting statements, and foreign repatriations. As foreign currencies may be used for financing purposes, we also asked about the frequency of transactions to arbitrage borrowing rates across currencies. Figure 5 reports the percentage of firms who “frequently” or “sometimes” transacted in the foreign currency derivatives markets for each of these reasons (expressed as a percentage of firms responding to the question for whom the exposure was applicable).The figure shows that the most frequently cited motivations for transacting in the foreign currency derivatives markets are for hedging near-term, directly observable exposures. The most commonly hedged exposures were On Balance Sheet Commitments (89% hedge frequently or sometimes), Anticipated Transactions expected within one year (85% hedge frequently or sometimes) and Foreign Repatriations (78% hedge frequently or sometimes)4. Identifiable off-balance sheet commitments are substantially less likely to be hedged by these firms than on balance sheet commitments. Anticipated transactions beyond one year are frequently hedged by 12% of the firms but sometimes hedged by 45%, suggesting that a majority of firms using foreign currency derivatives at least sometimes hedge exposures over a longer horizon. The more amorphous and longer term competitive exposure is hedged frequently by just 11% of firms but sometimes by an additional 28%, which is a noticeable increase from past surveys. Hedging translation exposure was a reason for currency derivatives transactions for only a minority of firms, with 14% percent doing this frequently and another 23% doing so sometimes. Finally, transacting in derivatives to hedge exposures from arbitraging interest rates across currencies was done frequently by only 5% of firms; however, 35% of firms indicated that they do this sometimes.C. Hedging IntensityNot much is known about the extent to which firms hedge their various exposures, so in this year’s survey we asked firms to indicate the percentage of the perceived exposure that they typically hedge across various categories of currency exposure. The responses were aggregated into four classes, firms that hedge 0-25%, 26-50%, 51-75%, and 76-100 of that particular exposure. Table 4 displays the percentage of firms that responded in each of the four groups for each of seven different categories of exposure. In each case, the percentages are taken only with Table 4: Percentage of Foreign Currency Exposures Typically HedgedPercentage of firms responding in the followingranges for the proportion of exposure hedged Exposure Category0-25%26-50%51-75%76-100%Average % of exposure hedgedOn-B/S Commitments40%13%12%35%49% Off-B/S Commitments72%11%5%13%23% Anticipated Transactions < 1 yr.42%22%9%27%42% Anticipated Transactions > 1yr.78%11%4%6%16% Economic/Competitive Exposure90%6%2%3%7% Translation of Foreign Accounts84%6%3%8%12% Repatriations50%14%5%31%40% Percentages taken of all responding firms that indicated that the exposure was applicable to them.4 Given that not all firms using currency derivatives have foreign operations from which to repatriate, these numbers suggest that an even larger proportion of the set of multinational firms use currency derivatives hedge foreign repatriations.respect to those responding firms that indicated that such an exposure was applicable in the previous question.The table reveals that with the exception of three types of exposure--on-balance sheet exposures, anticipated transactions less than one year and foreign repatriations--the majority of firms hedge less than 25% of their perceived exposures. Even for these three heavily hedged exposures, the average proportion hedged, shown in the final column of the table, is less than 50%. Only for on-balance sheet commitments does the average percentage of the exposure hedged reach 50%. Thus, partial hedging appears to be normal practice for these firms. Even in the cases of these three types of exposures, only a third of firms indicated that they hedged more than 75% of the total exposure. Again, these three were the more easily identifiable, near-term, transaction-based exposures. For longer term exposures such as anticipated transactions beyond one year and economic/competitive exposure, less than 10% of firms indicated that they hedged as much as 75% of the perceived exposure. These results suggest that foreign currency hedging, rather than eliminating exposures, generally only reduces the exposures, but typically by less than half of the original outstanding exposure.D. Maturity Structure of HedgingIn the 1995 survey, we learned that most firms use derivatives with short maturities. The percentage of firms using derivatives at longer maturities decreased significantly with the maturity of the derivatives; only 30% of firms reported any use of derivatives with tenor greater than three years. Again, we asked firms to provide some information on the maturity structure of their foreign currency hedging. Table 5 displays the results of our inquiry asking firms to indicate the percentage of their foreign currency hedging done with instruments of various original maturities.Table 5: Percentage of Hedging at Various MaturitiesProportion of responding firms indicating the percentage of theirtotal foreign currency derivatives with various original maturities Derivative Maturity0%1-25%26-50%51-75%76-100%1 day - 90 days18%23%26%13%21%91 – 180 days23%44%26%3%4%181 days - 1 year31%41%22%3%3%1 year – 3 years63%26%7%0%5%More than 3 years88%9%1%0%2% There are several interesting things to note about Table 5. First, short-term derivatives are used by a vast majority of firms. 82% of firms utilize foreign currency derivatives with an original maturity of 90 days or less, and 77% use foreign currency derivatives with an original maturity of 91 - 180 days for less while only 12% use foreign currency derivatives with maturities of more than 3 years. Second, firms tend to concentrate most of their foreign currency derivatives usage at the short horizon, especially 90 days or less. In fact, when we combine the responses in the first two rows, nearly one-quarter of the firms do all of their foreign currency derivative activity in instruments with original maturities of 180 days or less. Finally, the intensity of usage drops off dramatically with the lengthening of the maturity of the derivatives. Very few firms use any instruments with maturities over one year. There is a small group, 7%, of firms, all large firms,。
SCI 投稿全过程信件模板

SCI 投稿全过程信件模板SCI 投稿全过程信件模板一览一、最初投稿Cover letterDear Editors:We would like to submit the enclosed manuscript entitled “Paper Title”, which we wish to be considered for publication in “Journal Name”. No conflict of interest exits in the submission of this manuscript, and manuscript is approved by all authors for publication. I would like to declare on behalf of my co-authors that the work described was original research that has not been published previously, and not under consideration for publication elsewhere, in whole or in part. All the authors listed have approved the manuscript that is enclosed.In this work, we evaluated …… (简要介绍一下论文的创新性). I hope this paper is suitable for “Journal Name”.The following is a list of possible reviewers for your consideration:1) Name A E-mail: ××××@××××2) Name B E-mail: ××××@××××We deeply appreciate your consideration of our manuscript, and we look forward to receiving comments from the reviewers. If you have any queries, please don’t hesitate to contact me at the address below.Thank you and best regards.Yours sincerely,××××××Corresponding author:Name: ×××E-mail: ××××@××××二、催稿信Dear Prof. ×××:Sorry for disturbing you. I am not sure if it is the right time to contact you to inquire about the status of my submitted manuscript titled “Paper Title”. (ID: 文章稿号), although the status of “With Editor” has been lasting for more than two months, since submitted to journal three months ago. I am just wondering that my manuscript has been sent to reviewers or not?I would be greatly appreciated if you could spend some of your time check the status for us. I am very pleased to hear from you on the reviewer’s comments.Thank you very much for your consideration.Best regards!Yours sincerely,××××××Corresponding author:Name: ×××E-mail: ××××@××××三、修改稿Cover letterDear Dr/ Prof..(写上负责你文章编辑的姓名,显得尊重,因为第一次的投稿不知道具体负责的编辑,只能用通用的Editors):On behalf of my co-authors, we thank you very much for giving us an opportunity to revise our manuscript, we appreciate editor and reviewers very much for their positive and constructive comments and suggestions on our manuscript entitled “Paper Title”. (ID: 文章稿号).We have studied reviewer’s comments carefully and have made revision which marked in red in the paper. We have tried our best to revise our manuscript according to the comments. Attached please find the revised version, which we would like to submit for your kind consideration.We would like to express our great appreciation to you and reviewers for comments on our paper. Looking forward to hearing from you.Thank you and best regards.Yours sincerely,××××××Corresponding author:Name: ×××E-mail: ××××@××××四、修改稿回答审稿人的意见(最重要的部分)List of ResponsesDear Editors and Reviewers:Thank you for your letter and for the reviewers’ comments concerning our manuscript entitled “Paper Title” (ID: 文章稿号). Those comments are all valuable and very helpful for revising and improving our paper, as well as the important guiding significance to our researches. We have studied comments carefully and have made correction which we hope meet with approval. Revised portion are marked in red in the paper. The main corrections in the paper and the responds to the reviewer’s comments are as flowing: Responds to the reviewer’s comments:Reviewer #1:1. Response to comment: (……简要列出意见……)Response: ××××××2. Response to comment: (……简要列出意见……)Response: ××××××。
电影观后感模板英文

As a seasoned film critic, I often find myself reflecting on the myriad of emotions and thoughts that a movie can evoke. When crafting a film review, its essential to provide a comprehensive and engaging analysis that not only summarizes the plot but also delves into the artistic and technical aspects of the film. Heres a template for writing a film review in English that you can adapt to your own experiences and perspectives.Title: Movie Title A Cinematic Journey Through Theme/GenreIntroduction:Begin with a captivating hook that draws the reader in. This could be a powerful quote from the film, a brief anecdote, or an intriguing question. Follow this with a brief introduction to the film, including its title, director, main actors, and genre.Paragraph 1 Setting the Scene:Introduce the basic premise of the movie, providing enough detail to pique the readers interest without giving away any major spoilers. Mention the setting, time period, and the main characters involved.Paragraph 2 Plot Overview:Offer a concise summary of the plot, highlighting key events and the overall narrative structure. Be mindful not to reveal too much the goal is to give readers a sense of the story without ruining their experience.Paragraph 3 Character Analysis:Delve into the main characters, discussing their motivations, development, and the impact they have on the story. Consider the performances of the actors and how they bring the characters to life.Paragraph 4 Technical Aspects:Examine the films technical elements, such as cinematography, sound design, editing, and visual effects. Discuss how these elements contribute to the overall atmosphere and storytelling.Paragraph 5 Directors Vision:Analyze the directors approach to the film, including their stylistic choices and how these choices affect the audiences perception of the story. Consider the directors body of work and how this film fits within their oeuvre.Paragraph 6 Themes and Messages:Explore the deeper themes and messages of the film. Discuss how these themes are conveyed through the narrative, characters, and visual storytelling. Reflect on the relevance of these themes to contemporary society or universal human experiences.Paragraph 7 Personal Reflection:Share your personal reaction to the film. Discuss what resonated with you, any emotional responses you had, and how the film made you think or feel.Paragraph 8 Critique and Comparison:Offer a balanced critique of the film, pointing out both its strengths and weaknesses. Compare it to other films in the same genre or by the same director, if applicable.Conclusion:Summarize your overall impression of the film. Provide a final verdict on whether you would recommend the film to others and why. End with a memorable quote or thought that encapsulates the essence of the film.Additional Tips:Use descriptive language to paint a vivid picture for your readers.Incorporate specific examples from the film to support your points.Keep your tone conversational and engaging to maintain reader interest. Avoid using spoilers that would detract from a potential viewers experience.Remember, a film review is not just a summary of the plot its an exploration of the films artistic merit and its impact on the viewer. By following this template and adding your unique insights, you can craft a compelling and informative review that will engage and inform your readers.。
vibration2

Term Project (Vibration II)Jianfeng Zeng 000431182·IntroductionNowadays, in automotive industry, half-car model is always used to simulate the response of the car when it moves on the road, which can help to measure the amplitude of car vibration. So it is important to do this simulation due to improve the comfort ability and stability of the car. The straight way is to design the suspension properties which are stiffness and damping coefficient to make the car as smooth as possible when being drived on the rough road under changing conditions.The report is organized as follows. Firstly, set up a four-degree-of-freedom system half-car model. The model takes into account the body bounce, body pitch, and axle hop motion. And these motions can be formulated by setting four coordinates showed on the figure. Therefore, four equations of motion can be derived to find out the natural frequency of the system. After getting natural frequency, the forced vibration needs to be considered to figure out the damping coefficient of the suspension. Plug the parameters in these equations (the parameters are collected by papers and assumptions). Once these parameters are plugged in, the plots of response can be drawn by matlab in changing the vehicle speeds.·System modelingFigure 1 below is known as half-car model, which is the object of this research. As mentioned before, the system exists four coordinates which is marked in the figure 1.Fig.1 half-car model system(x1, x2, x3, θ are four coordinates of the system.)Table. 1 The parameters for the half-car model system (corresponding fig. 1)Sprung mass m1500kg Pitch moment of inertia I12700kgm2 Unsprung masses m236kg Damping coefficients(suspension) c1980Nsm-1 Damping coefficients(tire) c2100 Nsm-1 Suspension stiffness k116,000Nm-1 Tire stiffness k2160,000Nm-1 Distance of rear wheel to sprung mass c.g. l1 2.5m Distance of front wheel to sprung mass c.g. l2 1.5m Create the M-file in matlab and input the data of table.1. Run it before simulation by the equations of motion.·Free vibration analysisNatural frequency can be found by assuming damping c=0. According to the four coordinates of the system, four equations of motion can be derived by free body diagram (fig. 2) as follows:Fig.2 Free body diagram for free vibration=0 By following the equations of motion for the free vibration, structure the simulation(fig. 3) and set the initial conditions to x1(0)=0.1m, . After running it, the plots of responses are shown in scope block. And the figures of responses are list as fig.4.Fig.3 simulation for free vibrationFig.4 responses for free vibrationFig. 4 shows that the car body velocity and displacement responses have no decay under free vibration condition without damping, which proves the equations of motion correction. The reason why only analysis the responses for car body is that the parts of the system connect one by one, which means if there is certain decay in any one of the coordinates, the other three coordinates must be affected to decay. But now one of the coordinate has no decay. So the system has no decay at all for free vibration without damping.·Forced response (support motion)The motion of the base is considered as a harmonic function y(x)=Asin(2πx/L), which means ω=2πv/L, and assume the amplitude of the road is A=50mm and the length of one period should be L=20m. Same procedure with the free vibration analysis, four viscous damping nonlinear equations of motion can be derived by free body diagram (fig. 5) as follows:Fig.5 Free body diagram for forced vibration (support motion)The same process with the free vibration. Structure the simulation (fig.6) without initial conditions. After running it, the plots of responses are shown in scope block. And what should be concerned is the car body response, which has relative with the comfort ability and stability. The figures of responses are list as fig.7.Fig.6 simulation for forced vibrationFig.7(a) plot for displacement responseFig.7(b) plot for velocity response·Effects of vehicle speedThe former car velocity is set to 10m/s (36km/h). And now the car speed is set up to 20m/s (72km/h). The purpose is to find out what and how far is changing with the car speed. After inputting the different frequency in the sine wave function, the new plots are drawn as follows (fig.8):Fig.8(a) plot for displacement response(new)Fig.8(b) plot for velocity response(new)After comparing fig.7(a) to fig.8(a) and fig.7(b) to fig.8(b), it shows that with the speed increasing, the frequency increases a lot meanwhile the displacement response just goes up a little bit.·Summary & ConclusionFrom this test, we know how to make the car drive smoothly when driving on the rough road. We can achieve this goal by changing the properties of the suspension and choose the tire under certain specific situation. If the stiffness is set too high, the whole system will be like a rigid body which is fairly uncomfortable for the driver. On the other hand, what if the stiffness is set too low, the displacement will be higher, which can make car unstable and uncomfortable as well. Therefore, it is necessary to change the stiffness and damping coefficient again and again according to the plots we got until the response is within acceptable range.。
现代大学英语精读第二版book2unit4

• Match the words with their correct meaning in the text.
1. aesthetic 2. chaotic 3. commitment 4. standoff 5. impact a. a determination to do what one considers to be his duty b. being central or most important c. a situation in which neither side in a fight can gain an advantage d. the force of one object striking or hitting another e. concerning beauty, esp. beauty in art f. a stop or pause g. in a state of complete disorder and confusion
Background
Genre
– What kind of article do we have for this lesson? Where do we generally find it? – What does this article concentrate on? Does it focus on the description of the disaster or how people are saved? Why?
Background News Feature
• A news feature does not cover all the details a news report has to contain and only focuses on certain aspects of the event on which the reporter has something to say. Though the details about the news event have to be true and exact, the reporter can add his/her own comment and interpretation of the event, which he/she is not allowed in a news report.
GUIDANCE DOCUMENT FOR THE CONDUCT OF

Unclassified ENV/JM/MONO(2004)2 Organisation de Coopération et de Développement Economiques Organisation for Economic Co-operation and Development 05-Mar-2004 ___________________________________________________________________________________________English - Or. English ENVIRONMENT DIRECTORATE JOINT MEETING OF THE CHEMICALS COMMITTEE AND THE WORKING PARTY ON CHEMICALS, PESTICIDES AND BIOTECHNOLOGY GUIDANCE DOCUMENT FOR THE CONDUCT OF SKIN ABSORPTION STUDIES OECD SERIES ON TESTING AND ASSESSMENT Number 28 JT00159305 Document complet disponible sur OLIS dans son format d'origineComplete document available on OLIS in its original format ENV/JM/MONO(2004)2 Unclassified English - Or. EnglishENV/JM/MONO(2004)2OECD Environmental Health and Safety PublicationsSeries on Testing and AssessmentNo. 28GUIDANCE DOCUMENT FOR THE CONDUCT OFSKIN ABSORPTION STUDIESEnvironment DirectorateORGANISATION FOR ECONOMIC CO-OPERATION AND DEVELOPMENTParisMarch 20043ENV/JM//MONO(2004)2Also published in the Series on Testing and Assessment:No. 1, Guidance Document for the Development of OECDGuidelines for Testing of Chemicals (1993; reformatted 1995)No. 2, Detailed Review Paper on Biodegradability Testing(1995)No. 3, Guidance Document for Aquatic Effects Assessment(1995)No. 4, Report of the OECD Workshop on EnvironmentalHazard/Risk Assessment (1995)No. 5, Report of the SETAC/OECD Workshop on AvianToxicity Testing (1996)No. 6, Report of the Final Ring-test of the Daphnia magnaReproduction Test (1997)No. 7, Guidance Document on Direct Phototransformation ofChemicals in Water (1997)No. 8, Report of the OECD Workshop on Sharing Informationabout New Industrial Chemicals Assessment (1997)No. 9, Guidance Document for the Conduct of Studies ofOccupational Exposure to Pesticides during AgriculturalApplication (1997)No. 10, Report of the OECD Workshop on StatisticalAnalysis of Aquatic Toxicity Data (1998)No. 11, Detailed Review Paper on Aquatic Testing Methodsfor Pesticides and industrial Chemicals (1998)No. 12, Detailed Review Document on ClassificationSystems for Germ Cell Mutagenicity in OECD MemberCountries (1998)No. 13, Detailed Review Document on ClassificationSystems for Sensitising Substances in OECD MemberCountries 1998)No. 14, Detailed Review Document on ClassificationSystems for Eye Irritation/Corrosion in OECD MemberCountries (1998)No. 15, Detailed Review Document on ClassificationSystems for Reproductive Toxicity in OECD MemberCountries (1998)4ENV/JM/MONO(2004)2 No. 16, Detailed Review Document on ClassificationSystems for Skin Irritation/Corrosion in OECD MemberCountries (1998)No. 17, Environmental Exposure Assessment Strategies forExisting Industrial Chemicals in OECD Member Countries(1999)No. 18, Report of the OECD Workshop on Improving theUse of Monitoring Data in the Exposure Assessment ofIndustrial Chemicals (2000)No. 19, Draft Guidance Document on the Recognition, Assessment and Use of Clinical Signs as Humane Endpointsfor Experimental Animals used in Safety Evaluation (1999)No. 20, Revised Draft Guidance Document for Neurotoxicity Testing (in preparation)No. 21, Detailed Review Paper: Appraisal of Test Methodsfor Sex Hormone Disrupting Chemicals (2000)No. 22, Guidance Document for the Performance of Out-door Monolith Lysimeter Studies (2000)No. 23, Guidance Document on Aquatic Toxicity Testing ofDifficult Substances and Mixtures (2000)No. 24, Guidance Document on Acute Oral Toxicity Testing(2001)No. 25, Detailed Review Document on Hazard Classification Systems for Specifics Target Organ SystemicToxicity Repeated Exposure in OECD Member Countries(2001)No. 26, Revised Analysis of Responses Received fromMember Countries to the Questionnaire on Regulatory AcuteToxicity Data Needs (2001)No 27, Guidance Document on the Use of the HarmonisedSystem for the Classification of Chemicals Which are Hazardous for the Aquatic Environment (2001)No 28, Guidance Document for the Conduct of Skin Absorption Studies (2004)No 29, Draft Guidance Document on Transformation/Dissolution of Metals and Metal Compoundsin Aqueous Media (2001)5ENV/JM//MONO(2004)2No 30, Detailed Review Document on HazardClassification Systems for Mixtures (2001)No 31, Detailed Review Paper on Non-GenotoxicCarcinogens Detection: The Performance of In-Vitro CellTransformation Assays(draft)No. 32, Guidance Notes for Analysis and Evaluation ofRepeat-Dose Toxicity Studies (2000)No. 33, Harmonised Integrated Classification System forHuman Health and Environmental Hazards of ChemicalSubstances and Mixtures(2001)No. 34, Guidance Document on the Development,Validation and Regulatory Acceptance of New and UpdatedInternationally Acceptable Test Methods in HazardAssessment (in preparation)No. 35, Guidance notes for analysis and evaluation ofchronic toxicity and carcinogenicity studies (2002)No. 36, Report of the OECD/UNEP Workshop on the use ofMultimedia Models for estimating overall EnvironmentalPresistence and long range Transport in the context ofPBTS/POPS Assessment (2002)No. 37, Detailed Review Document on ClassificationSystems for Substances Which Pose an Aspiration Hazard(2002)No. 38, Detailed Background Review of the UterotrophicAssay Summary of the Available Literature in Support of theProject of the OECD Task Force on Endocrine DisruptersTesting and Assessment (EDTA) to Standardise and Validatethe Uterotrophic Assay (2003)No. 39, Guidance Document on Acute Inhalation ToxicityTesting (in preparation)No. 40, Detailed Review Document on Classification inOECD Member Countries of Substances and Mixtures WhichCause Respiratory Tract Irritation and Corrosion (2003)No. 41, Detailed Review Document on Classification inOECD Member Countries of Substances and Mixtures whichin Contact with Water Release Toxic Gases (2003)No. 42, Draft Guidance Document on Reporting SummaryInformation on Environmental, Occupational and ConsumerExposure (in preparation)6ENV/JM/MONO(2004)2 No. 43, Draft Guidance Document on Reproductive ToxicityTesting and Assessment (in preparation)No. 44, Descriptions of Selected Key Generic Terms used inChemical Hazard/Risk Assessment (2003)No. 45, Draft Guidance Document on the Use of MultimediaModels for Estimating Overall Environmental Persistence andLong-range Transport (2004)© OECD 2003Applications for permission to reproduce or translate all orpart of this material should be made to: Head of PublicationsService, OECD, 2 rue André-Pascal, 75775 Paris Cedex 16,France7ENV/JM//MONO(2004)2About the OECDThe Organisation for Economic Co-operation and Development (OECD) is an intergovernmental organisation in which representatives of 30 industrialised countries in North America, Europe and the Pacific, as well as the European Commission, meet to co-ordinate and harmonise policies, discuss issues of mutual concern, and work together to respond to international problems. Most of the OECD's work is carried out by more than 200 specialised Committees and subsidiary groups composed of Member country delegates. Observers from several countries with special status at the OECD, and from interested international organisations, attend many of the OECD's Workshops and other meetings. Committees and subsidiary groups are served by the OECD Secretariat, located in Paris, France, which is organised into Directorates and Divisions.The work of the OECD related to chemical safety is carried out in the Environment, Health and Safety Programme. As part of its work on chemical testing, the OECD has issued several Council Decisions and Recommendations (the former legally binding on member countries), as well as numerous Guidance Documents and technical reports. The best known of these publications, the OECD Test Guidelines, is a collection of methods used to assess the hazards of chemicals and of chemical preparations. These methods cover tests for physical and chemical properties, effects on human health and wildlife, and accumulation and degradation in the environment. The OECD Test Guidelines are recognised world-wide as the standard reference tool for chemical testing.More information about the Environment, Health and Safety Programme and its publications (including the Test Guidelines) is available on the OECD’s World Wide Web site /ehs/.The Environment, Health and Safety Programme co-operates closely with other international organisations. This document was produced within the framework of the Inter-Organisation Programme for the Sound Management of Chemicals (IOMC).The Inter-Organization Programme for the Sound Management of Chemicals (IOMC) was established in 1995 by UNEP, ILO, FAO, WHO, UNIDO and the OECD (the Participating Organisations), following recommendations made by the 1992 UN Conference on Environment and Development to strengthen co-operation and increase international co-ordination in the field of chemical safety. UNITAR joined the IOMC in 1997 to become the seventh Participating Organisation. The purpose of the IOMC is to promote co-ordination of the policies and activities pursued by the Participating Organisations, jointly or separately, to achieve the sound management of chemicals in relation to human health and the environment.8ENV/JM/MONO(2004)2This publication is available electronically, at no charge.For the complete text of this and many other Environment,Health and Safety publications, consult the OECD’sWorld Wide Web site(/document/21/0,2340,en_2649_34365_1862357_1_1_1_1,00.html)or contact:OECD Environment Directorate,Environment, Health and Safety Division2 rue André-Pascal75775 Paris Cedex 16FranceFax: (33-1) 45 24 16 75E-mail: ehscont@9ENV/JM//MONO(2004)2TABLE OF CONTENTSHISTORY OF THE DOCUMENT (11)INTRODUCTION (12)INITIAL CONSIDERATIONS (12)PRINCIPLE OF THE TESTS (13)DESCRIPTION OF THE METHODS (14)Selection of Animal Species (14)Number and Sex of Animals (14)Housing and Feeding Conditions (14)Preparation of Animals (14)Diffusion Cell (15)Receptor Fluid (15)Maintenance of Metabolic Activity (16)Skin Preparations (16)Skin Preparation Integrity (17)Test Substance (18)Test Preparation (19)Application to the Skin (19)Occlusion Conditions (20)Temperature (21)Terminal Procedures (22)Analysis (23)REFERENCES (25)GLOSSARY OF TERMS (30)10HISTORY OF THE DOCUMENT1. The development of the OECD Guidance Document for the conduct of skin absorption studies was agreed at a meeting of the OECD Extended Steering Group on the Development of in vitro and in vivo Percutaneous Absorption Methods as Test Guidelines in Research Triangle Park, NC, USA in October 1997. The need for the Guidance Document became apparent when national experts from a number of Member countries could not agree on the possibilities and limitations of the in vitro test. A Guidance Document was considered the most suitable option to explain Member country’s concerns and options for use of the in vitro method.2. The Toxicology Expert Group of the European Crop Protection Association (ECPA) volunteered to assist in the preparation of a first draft of the Guidance Document, in addition to the revision of the draft Test Guidelines. A sub-group of technical experts from ECPA was formed to undertake this task.3. Several meetings of the ECPA Dermal Absorption Group took place during 1998 and 1999. In preparing the first draft suggestions made at the Meeting of the OECD National Co-ordinators of the Test Guidelines Programme in August 1998 (WNT 10) and at an OECD Steering Group Meeting in Lodz, Poland in June 1999 were taken into account.4. In November 1999, an OECD Expert Group was established to reconsider the first draft and to gain consensus on specific aspects of the Guidance Document. This Expert Group comprised Angela Auletta (US EPA); Bob Bronaugh (US FDA); Jon Heylings (Syngenta, UK); John Worgan (Health Canada)and Errol Zeiger. The OECD Secretariat provided practical assistance and technical guidance.5. Following further discussion between the WNT and the technical expert groups, the revised Guidance Document was presented to the National Co-ordinators meeting in May 2000 (WNT 12). The Guidance Document was endorsed and declassified by the Joint Meeting of the Chemicals Committee and Working Group on Chemicals, Pesticides and Biotechnology in May 2001.6. The Guidance Document provides additional technical background to both the in vivo and in vitro methods for skin absorption as described in Test Guidelines 427 and 428 respectively. It further highlights explains the use of the outcome of these tests for hazard and risk assessment purposes.INTRODUCTION7. Some of the procedures required by the OECD guidelines for the conduct of skin absorption studies (OECD 427 and 428) need further detail before scientists unfamiliar with such procedures can undertake these types of studies correctly. Furthermore, some users of the guidelines from specific industries (e.g. cosmetic, detergent and agrochemical) may have requirements, which are difficult to embody within guidelines of broad applicability. For these reasons, this guidance document has been prepared to support technical aspects of the OECD skin absorption test guidelines (1) (2).INITIAL CONSIDERATIONS8. The methods for measuring skin absorption and dermal delivery can be divided into two categories: in vivo and in vitro. In vivo methods in experimental animals have traditionally been used to assess skin absorption for regulatory purposes and national guidelines are available (3)(4)(5). They have some advantages over in vitro methods, which include generation of systemic kinetic and metabolic information. The disadvantages are the use of live animals, the need for radio-labelled material to facilitate reliable results, difficulties in determining the early absorption phase and the differences in permeability of the preferred species (rat) and human skin. Animal skin is generally more permeable and therefore may overestimate human percutaneous absorption (6)(7)(8).in vitro method has been described in detail in a number of monographs published over9. Thea 10-year period (9)(10)(11)(12)(13)(14)(15)(16)(17). The advantages of the in vitro method over the in vivo method are that it can be used equally well with skin from humans and other species; several replicate measurements can be made from the same or a number of different subjects; live animals are not used; intended use exposure conditions can be studied; a wider range of physical forms can be investigated (i.e. including solids and granules); the impact of skin damage on absorption can be assessed avoiding ethical issues. In addition, the in vitro method can be used for non-radio-labelled test substances, which are extensively metabolised. A limitation associated with an in vitro approach is that sink conditions of the peripheral blood flow may not be fully reproduced. However, skin absorption is primarily a passive process and studies undertaken using appropriate in vitro experimental conditions have produced data for a wide range of chemicals that demonstrate the usefulness of this method (18)(19)(20)(21)(22)(23) (24)(25)(26)(27)(28)(29) (30)(31)(32)(33)(34)(35) (36)(37)(38)(39)(40)(41). Such methods have found use in, for example, comparing delivery of chemicals into and through skin from different formulations and can also provide useful models for the assessment of risk due to percutaneous absorption in humans.10. National regulatory authorities may have different preferences for in vivo and/or in vitro skin absorption studies, so the choice of which method(s) to use should be in line with the requirements of the specific regulatory bodies/authorities. The use of in vivo and/or in vitro methods will depend on the situation being evaluated. In vivo data may be used alone. However, it may be possible, depending on the intended use of the test substance, to undertake only an in vitro study for a first evaluation of skin penetration. Where a more refined evaluation of dermal absorption is required, in vitro and in vivo data are considered together. It is advisable to contact the appropriate regulatory authority to confirm the most relevant and adequate test protocol(s) before conducting skin absorption studies.11. The principle factors relating to the experimental conditions for in vitro skin absorption studies are discussed in detail in this guidance document.12. To demonstrate the performance and reliability of the test system in the performing laboratory, the results for relevant reference chemicals should be available and in agreement withpublished literature, e.g. (12) for the method used. This alternative could be met by testing an appropriate reference substance (preferably of a lipophilicity close to the test substance) concurrently with the test substance or by providing adequate historical data for a number of reference substances of different lipophilicity (Typical examples of reference chemicals that have been extensively studied in vivo and in vitro, include caffeine (log P ow 0.01), benzoic acid (log P ow 1.83) and testosterone (log P ow 3.32).PRINCIPLE OF THE TESTS13. The methods for measuring dermal absorption and dermal delivery can be divided into two categories: in vivo and in vitro. The methods study the diffusion of a test substance from a test preparation through the skin barrier and into the skin.In Vivo14. The rat is the most commonly used species for the in vivo test. In rats, the application site should be about 10 cm2 and should be defined by a device, which is secured on the skin surface. The test preparation is applied to the surface of skin. The test preparation remains on the skin for a specified period of time, relating to potential human exposure, then the test preparation is removed by an appropriate cleansing procedure. During exposure, animals are housed individually in metabolism cages from which excreta are collected. When appropriate, exhaled volatile metabolites may also be collected. At the end of the study, the animals may be anaesthetised and the dislodgeable dose is washed from the skin surface. The animals are then terminated and the amount of dose associated with the skin, the carcass and in excreta, is determined. These data allow for an estimate of the total recovery of the test substance. The skin absorption of the test substance can be expressed as the percentage of dose absorbed per unit time or in terms of an average absorption rate per unit area of skin (e.g., µg/cm2/hr).15. Test substance remaining in the skin after wash-off will normally move over time by one of three processes, it may be sloughed off by desquamation, it may be ingested when the animal grooms itself, or it may diffuse into the animal to become systemically available. Suitable technical procedures will be required to prevent grooming of the treated site, and to prevent desquamed skin from falling into the urine/faeces collection system. This is crucial to avoid overestimation of the systemically absorbed dose.In Vitro16. Skin from many mammalian species, including humans, can be used. Several references discuss interspecies comparison of dermal absorption and absorption through different skin regions (11)(12)(16)(25). The test preparation is applied to the surface of excised skin, which is mounted in a diffusion cell. The receptor fluid, which must have an adequate capacity to solubilise the test substance, is maintained in contact with the underside of the skin from the time of application until the end of the collection of the receptor fluid. The test preparation remains on the skin for a specified period of time, relating to potential human exposure, and then the test preparation is removed by an appropriate cleansing procedure. The receptor fluid is sampled at time points throughout the experiment to ascertain the mass (and possibly rate) of the test substance (including any significant metabolite) passing through the skin. At the end of the study, the dislodgeable dose, the amount associated with the skin and the amount in the receptor fluid is determined. These data are necessary to calculate the total skin absorption, and allow for an estimate of the total recovery of the test substance.The skin absorption of the test substance is expressed as the percentage, amount or rate of absorption per unit time, or in the case of infinite dose applications, as a permeability constant. DESCRIPTION OF THE METHODSSelection of Animal SpeciesIn Vivo17. No further guidance considered necessaryIn Vitro18. Skin preparations from human or animal sources including hairless animals can be used (9)(10)(11)(12)(13)(14)(15)(33)(35)(36)(37). Reconstituted human skin models can be used if data from reference chemicals are consistent with those in the published literature. In most cases, these studies are intended to predict skin absorption in humans. Therefore, use of human skin is most relevant. However, for regulatory or practical reasons, other animal species may have to be used, usually rats or pigs. Rat skin is often used because the majority of regulatory toxicity studies are in this species. Furthermore, existing legislation for assessment of in vivo dermal absorption also stipulate the use of this species (3)(4)(5). Pig skin is used because of its similarity to human skin in terms of its morphology and permeability characteristics, making it a practical alternative (13). Viable skin is preferred but it is recognised that viable skin is not always easily available. Furthermore, for reasons of comparability, standardised non-viable skin may have advantages. Therefore, non-viable skin can also be used provided that the integrity of the skin can be demonstrated.Number and Sex of AnimalsIn Vivo19. No further guidance considered necessaryHousing and Feeding ConditionsIn Vivo20. No further guidance considered necessary.Preparation of AnimalsIn Vivo21. Animals should be housed and fed according to conventional procedures. An acclimatisation period of at least 5 days prior to the application of the test preparation is required. Feed and water should be freely available (15). At least 16 hours before treatment, the application site should be prepared. For example, in the rat, hair on the shoulders and back is removed with animal hair clippers (1)(15). Care is taken to ensure that the skin surface is free of abrasions, which will inevitably increasepercutaneous absorption (15). Consequently shaving of the application site should be avoided. The area must be large enough to allow reliable calculation of the absorbed amount of test substance per cm2 skin, preferably at least 10 cm2 (15). The skin may be washed prior to dosing with a suitable medium, such as water or a mild detergent (15). However, careful consideration must be given to the possibility of modifying the skin or its barrier properties. The reasons for washing and the choice of the cleansing agent should be justified in the report. After preparation, the animals are placed in their individual metabolism cages. Before application animals are marked to permit individual identification.Diffusion CellIn Vitro22. There are several types of diffusion cells, which all consist of a donor chamber and a receptor chamber between which the skin is positioned (9)(11)(16)(45)(46). In a set of diffusion cells, each cell has the same surface area, typically in the range 0.3 to 5 cm2. The skin preparation is positioned in the cell with the stratum corneum uppermost. The cell should provide a good seal around the skin, and enable good mixing and easy sampling of the receptor fluid. It must be made from an inert material, such as glass or PTFE, to minimise interaction with the test substance. The design of the cells should allow accurate temperature control of the skin and receptor fluid. Either static or flow-through cells are acceptable (43)(45)(46)(47). Donor chamber design should allow for occlusion and easy removal of the test preparation, if required.Receptor FluidIn VitroAcceptable Receptor Fluids23. The selection of the receptor fluid must be compatible with the skin preparation and meet the key parameter of solubility of the test substance (not the test preparation) in the receptor fluid.24. For non-viable skin preparations, the receptor fluids for evaluating water soluble compounds are usually saline solutions, pH 7.4. For lipophilic test substances the receptor fluid can contain organic solvents such as 1:1 ethanol:water or 6% polyethylene glycol 20 oleyl ether in water (12)(13). Barrier integrity of skin must not be damaged. Thin preparations of sections of skin (200 µm) have been shown to have increased susceptibility to barrier damage.25. For metabolically active skin preparations, a physiological based receptor fluid, e.g. tissue culture medium should be used (10)(11)(12)(13)(14)(15). The limitation of using such a receptor fluid for non-polar test substances is the potential inadequate solubility of the test substance in the receptor fluid. This should be addressed by the addition of a modifier to the physiological fluid, typically 6% polyethylene glycol 20 oleyl ether (47) or 5% bovine serum albumin (15).26. For non-radio-labelled test substances the chosen receptor fluid should not interfere with the analytical methods employed.Solubility of Test Substance in Receptor Fluid27. It is imperative that solubility in the receptor fluid is not a rate-determining step in skin absorption (12)(13)(15)(16).28. Normally in flow-through systems an adequate flow rate of receptor fluid will prevent back diffusion. In static systems it is particularly important to ensure satisfactory solubility. Ideally, an estimate of the likely maximum achieved concentration of test substance in receptor fluid should be made, based on previous in vivo or in vitro study data. Physicochemical data or experimental results should be used to show that about 10 times this concentration is achievable under the experimental conditions (15)(16). This will ensure that back diffusion is not significant. The absorption rate profile over the study duration can be used retrospectively to confirm that solubility was not rate limiting.Receptor Fluid Exchange /Flow Rate29. In static cells, the volume of the specific receptor fluid (typically 2-20 ml) has to be sufficient not to limit the dermal absorption of the test substance (44).30. In flow-through cells the volume of the receptor fluid in the receptor chamber beneath the skin is typically 0.1 - 5 ml. This volume must be replenished continuously such that the rate of diffusion into the receptor fluid does not become a rate-limiting step (44).31. Typical flow rates may be 9 ml/hr for a receptor chamber of 3 ml (3 changes/hr), or 1.5 mL /hr for a receptor chamber of 150-300 µL (5-10 changes/hr). A slower flow rate (i.e., representing ~1 change/hr) may sometimes be appropriate, particularly in the case of unlabeled test compound where analytical difficulties may result if the concentration of test material in receptor fluid is too low.Maintenance of Metabolic Activity32. Metabolic activity is not required to study dermal absorption of radio-labelled material, as this is a passive diffusion process. However, the biotransformation of the compound in skin prior to systemic absorption may be significant for some compounds. Where maintenance of metabolic activity is required (i.e., there is a requirement for the identification of metabolites) it is necessary to demonstrate that the relevant metabolic activity is maintained for the duration of the study (11)(15). 33. It is known that changes occur in the metabolic capacity of skin once excised from the donor (11)(15). There is usually a rapid reduction in glutathione and phase I enzymes, and a slower reduction in energy metabolism. Some enzyme systems such as esterases remain active even after freezing. The use of cell culture media or other physiologically compatible receptor fluids will increase the duration of metabolic activity. The most widely used indicator of skin viability has been glucose utilization (48) since energy metabolism is critical to metabolic integrity.34. The degree of metabolism in vitro will depend on the residence time of the test substance in the metabolically active tissue layer (normally the epidermis). A longer residence time provides greater opportunity for metabolism; residence time is often shorter in vivo than in vitro (16).Skin PreparationsIn Vitro35. Dermal absorption through human skin is normally assessed using abdominal or breast skin (12)(14)(15)(49)(50)(51). When pig skin is used, the most commonly used sites are the flank and the ear; skin from the back or limb is also typically used (13).36. In the case of the rat, dorsal or ventral skin may be used but dorsal skin should be used if the study is designed to correspond with the in vivo application site (12).。
五年级作文调查你们班上上网的信息,五百字

五年级作文调查你们班上上网的信息,五百字示例回答如下1:Title: A Survey on Internet Usage in Our Fifth Grade ClassIntroduction:In today's digital age, the internet has become an integral part of our lives, including the lives of fifth-grade students. This article aims to explore the internet usage habits of our class and shed light on the benefits and challenges associated with online activities.Survey Methodology:To conduct this survey, a questionnaire was distributed among the students in our fifth-grade class. The questionnaire consisted of questions regarding the frequency and purpose of internet usage, preferred online platforms, and any concerns related to online safety.Survey Findings:Based on the responses received, the following trends were observed:1. Frequency of Internet Usage:- 80 of the students reported using the internet every day, primarily for educational purposes.- 15 of the students stated that they use the internet a few times a week, mainly for entertainment purposes.- Only 5 of the students reported using the internet occasionally or rarely.2. Purpose of Internet Usage:- Educational purposes, such as research for school assignments and accessing online learning resources, were the primary reasons for internet usage among our class.- Social media and online gaming were also popular activities, with 20 of students using the internet for these purposes.3. Preferred Online Platforms:- Google was the most commonly used search engine, with 70 of students relying on it for research purposes.- YouTube was the preferred platform for watching educational videos, with 50 of students utilizing it.- For online gaming, popular platforms included Roblox, Minecraft, and Fortnite.4. Concerns related to Online Safety:- 60 of students expressed concerns about encountering inappropriate content online.- 40 of students worried about cyberbullying and online privacy.- However, it was reassuring to note that 90 of students reported discussing online safety with their parents or teachers.Conclusion:The survey results indicate that internet usage among fifth-grade students in our class is primarily for educational purposes, with a significant focus on research and accessing online learning resources. However, concerns related to online safety, such as exposure to inappropriate content and cyberbullying, are prevalent. It is crucial for parents and teachers to continue educating students about responsible internet usage and ensuring their safety online.标题:我们五年级班上网信息调查简介:在当今数字时代,互联网已成为我们生活的一部分,包括五年级学生的生活。
调查了这件事情英文作文

调查了这件事情英文作文下载温馨提示:该文档是我店铺精心编制而成,希望大家下载以后,能够帮助大家解决实际的问题。
文档下载后可定制随意修改,请根据实际需要进行相应的调整和使用,谢谢!并且,本店铺为大家提供各种各样类型的实用资料,如教育随笔、日记赏析、句子摘抄、古诗大全、经典美文、话题作文、工作总结、词语解析、文案摘录、其他资料等等,如想了解不同资料格式和写法,敬请关注!Download tips: This document is carefully compiled by theeditor. I hope that after you download them,they can help yousolve practical problems. The document can be customized andmodified after downloading,please adjust and use it according toactual needs, thank you!In addition, our shop provides you with various types ofpractical materials,such as educational essays, diaryappreciation,sentence excerpts,ancient poems,classic articles,topic composition,work summary,word parsing,copyexcerpts,other materials and so on,want to know different data formats andwriting methods,please pay attention!Recently, I conducted a survey on the topic and found some interesting results. Many people expressed strong opinions on the matter, with some feeling very passionate about it. It was clear that this issue struck a chord with a lot of individuals.One thing that stood out to me was the diversity of responses I received. People from all walks of life and backgrounds had something to say about the topic. It was fascinating to see how different perspectives shaped their views on the matter.I was surprised by the level of knowledge people had about the issue. Some were very well-informed and had clearly done their research, while others seemed to have a more limited understanding. It was a good reminder of the importance of education and awareness when it comes to such topics.There were also a few unexpected findings that came out of the survey. Some people had unique experiences related to the issue that I hadn't considered before. It was eye-opening to hear these personal stories and added an extra layer of complexity to the topic.Overall, the survey provided a lot of valuable insight into the matter. It highlighted the diversity of opinions, the importance of education, and the power of personal experiences in shaping our views. It was a great reminder of the richness and complexity of human perspectives.。
群众反馈英语作文

群众反馈英语作文Feedback from the Masses。
In recent years, the Chinese government has been actively promoting the concept of "mass line education and practice", which emphasizes the importance of listening to and responding to the needs and opinions of the masses. As a result, the government has been encouraging citizens to provide feedback on a wide range of issues, from policy decisions to public services.One of the most common forms of feedback is through online platforms, such as social media and government websites. These platforms allow citizens to share their opinions and suggestions with a wider audience, and to engage in discussions with government officials and other members of the public. For example, in 2019, the Ministry of Education launched an online survey to gather feedback on the national college entrance exam, which received over 2 million responses.Another way that citizens provide feedback is through public meetings and consultations. These events are often organized by government agencies or local authorities, and provide an opportunity for citizens to express their opinions and concerns directly to officials. For example, in 2018, the city of Shanghai held a public consultation on a proposed ban on disposable plastic products, which attracted over 1,000 participants.The government also encourages citizens to report problems and issues directly to officials through hotlines and other channels. These reports can range from complaints about public services, such as garbage collection and transportation, to concerns about safety and security. For example, in 2017, the city of Beijing launched a "smart city" initiative that included a hotline for citizens to report problems with public services, which received over 6 million calls in its first year.Overall, the feedback from the masses plays an important role in shaping government policies and improvingpublic services. By listening to the needs and concerns of citizens, the government can better understand the challenges facing society and develop effective solutions to address them. At the same time, citizens have a responsibility to provide constructive feedback and to engage in dialogue with officials to ensure that their voices are heard and their needs are met.仿写:In recent years, the Chinese government has been actively promoting the concept of "mass line education and practice", which emphasizes the importance of listening to and responding to the needs and opinions of the masses. As a result, the government has been encouraging citizens to provide feedback on a wide range of issues, from policy decisions to public services.One of the most common forms of feedback is through online platforms, such as social media and government websites. These platforms allow citizens to share their opinions and suggestions with a wider audience, and toengage in discussions with government officials and other members of the public. For example, in 2019, the Ministryof Health launched an online survey to gather feedback on the national health insurance system, which received over 5 million responses.Another way that citizens provide feedback is through public meetings and consultations. These events are often organized by government agencies or local authorities, and provide an opportunity for citizens to express their opinions and concerns directly to officials. For example,in 2020, the city of Guangzhou held a public consultationon a proposed ban on single-use plastic bags, whichattracted over 2,000 participants.The government also encourages citizens to report problems and issues directly to officials through hotlines and other channels. These reports can range from complaints about public services, such as water supply and electricity, to concerns about environmental pollution and traffic congestion. For example, in 2021, the city of Shenzhen launched a "smart city" initiative that included a hotlinefor citizens to report problems with public services, which received over 10 million calls in its first year.Overall, the feedback from the masses plays an important role in shaping government policies and improving public services. By listening to the needs and concerns of citizens, the government can better understand the challenges facing society and develop effective solutions to address them. At the same time, citizens have a responsibility to provide constructive feedback and to engage in dialogue with officials to ensure that their voices are heard and their needs are met.。
- 1、下载文档前请自行甄别文档内容的完整性,平台不提供额外的编辑、内容补充、找答案等附加服务。
- 2、"仅部分预览"的文档,不可在线预览部分如存在完整性等问题,可反馈申请退款(可完整预览的文档不适用该条件!)。
- 3、如文档侵犯您的权益,请联系客服反馈,我们会尽快为您处理(人工客服工作时间:9:00-18:30)。
SUMMARY OF RESPONSES RECEIVED ON THE REPORT “CREDIT RISK MODELLING: CURRENT PRACTICES AND APPLICATIONS”Basel Committee on Banking SupervisionBaselMay 2000Models Task ForceChairperson:Danièle Nouy, Secretary GeneralBasel Committee on Banking SupervisionCommission Bancaire et Financière, Brussels Patrick Massin Office of the Superintendent of Financial Institutions, Ottawa Richard Gresser Commission Bancaire, Paris Thierry BayleChristian DelhommeEvelyn GuillyC. K. Tran Deutsche Bundesbank, Frankfurt am Main Stefan Hohl(ex-officio member)Roland Raskopf Bundesaufsichtsamt für das Kreditwesen, Berlin Uwe TraberBanca d’Italia, Rome Sebastiano Laviola The Bank of Japan, Tokyo Masao Yoneyama (ex-officio member)Akira IedaFinancial Supervisory Agency, Tokyo Taro TeruuchiTomomichi Tomiie Commission de Surveillance du Secteur Financier, Luxembourg Erik OschDe Nederlandsche Bank N.V., Amsterdam Jaap BikkerAd HuijserBanco de Espana, Madrid Javier de La CruzFernando Vargas Finansinspektionen, Stockholm Lena BengtssonMats Stenhammar (ex-officio member)Karin Sandstrom Eidgenössische Bankenkommission, Bern Uwe Steinhauser Swiss National Bank, Zürich Christian Braun Financial Services Authority, London Terry AllenAlan CathcartColin Tattersall Bank of England, London Pamela NickellVictoria SaportaBoard of Governors of the Federal Reserve System,James Houpt Washington, DC David JonesBill Treacy Federal Reserve Bank of New York,Brian PetersNew York Marc Saidenberg Office of the Comptroller of the Currency, Washington, DC Daniel BaileySteve JacksonRoger Tufts Federal Deposit Insurance Corporation, Washington, DC Miguel BrownJohn Feid Secretariat of the European Commission Jürgen Baum Secretariat of the Basel Committee on Banking Zahra El-Mekkawy Supervision, Bank for International SettlementsTable of ContentsBackground (1)The responses (1)Overview of Conceptual Approaches to Credit Risk Modelling (2)Parameter Specification and Estimation (3)Validation (4)Supervisory and regulatory application of models (4)BackgroundIn April 1999, The Basel Committee on Banking Supervision published a report, prepared by the Basel Models Task Force, entitled “Credit Risk Modelling: Current Practices and Applications”. This report analysed current practices and issues in credit risk modelling and assessed the potential use of credit risk models for supervisory and regulatory purposes. The report concluded that “credit risk modelling may indeed prove to result in better internal risk management, and may have the potential to be used in the supervisory oversight of banking organisations. However, before a portfolio modelling approach could be used in the formal process of setting regulatory capital requirements for credit risk, regulators would have to be confident not only that models are being used to actively manage risk, but also that they are conceptually sound, empirically validated and produce capital requirements that are comparable across institutions. At this time, significant hurdles, principally concerning data availability and model validation, still need to be cleared before these objectives can be met, and the Committee sees difficulties in overcoming these hurdles in the timescale envisaged for amending the Capital Accord.”The report went on to set out current practice in credit risk modelling, based on a survey of 20 large international banks in 10 countries. Technical aspects of modelling were examined and the modelling issues that they raised were discussed. Comments from interested parties were sought by 1 October 1999.The responsesTwenty-two responses were received. Three were received from non-G-10 banking regulators, nine from individual banks or industry associations, five from academics or academic organisations and five from representatives of the consulting, accounting or risk management professions. The Models Task Force held discussions with two of the main industry associations in October 1999.The Committee is grateful to all those who responded. The Committee values the comments made and the interest shown in the report. The responses acknowledged that the report addressed the relevant issues in a serious and balanced manner. The respondents were supportive of the argument that data shortages made parameter estimation difficult, particularly estimates of the tails of distributions, correlations, and loss given default.Not all of the responses agreed with all the judgements within the report, however. The chief issues on which respondents challenged the report were on validation, where some respondents felt that the Committee was searching for a market risk-style backtesting framework, and on comparability of outputs, where some respondents took the report to indicate a wish for uniformity of outputs and argued that this was neither achievable nor desirable.Many technical insights and comments were received on the report. In addition, some respondents submitted descriptions of modelling activity in their own domains, and some provided papers and original research that addressed general credit modelling issues. Regrettably, this summary cannot reflect all the comments received; accordingly, the summary focuses on responses that comment directly on the report.The purpose of the report was to facilitate debate and discuss the issues surrounding credit risk modelling, and not to provide proposals for a capital regime. Since details of a portfolio models-based capital regime are not presently under active consideration, the Committee does not wish to react in detail to comments made.At this stage, the Committee wishes to note that a robust validation process will be needed to ensure the integrity of any future internal model based regime; the Committee is open minded as to the form of validation. On the need for comparability of outputs, the Committee has noted the points made by respondents, but notes that a capital regime will need to provide a level playing field for capital requirements in banking organisations.There have been a number of subsequent developments since the report was issued. Chief among these from the Committee’s point of view was the issuance of a consultative paper entitled “A New Capital Adequacy Framework” in June 1999. Inter alia, the consultative paper proposed making use of banks’ internal credit rating systems, an important input to many forms of credit risk model, and the Models Task Force is currently engaged in significant work in developing options for an internal ratings based approach to minimum capital requirements. The consultative paper did, however, make reference to credit risk modelling in the light of April’s report, saying that the Committee hoped the outputs of such models could at some future time be used as a basis for setting regulatory capital requirements. In addition, the industry has co-operated with the Committee on empirical work related to the development of an internal ratings-based approach. Moreover, new material on the subject of models continues to be published: a recent example is a joint IIF/ISDA study entitled “Modelling Credit Risk” (February 2000). The Committee expects that its work on internal ratings and the associated work in co-operation with the industry will help pave the way for a future models based approach to credit risk capital requirements.The remainder of this note is devoted to a summary of the responses received on the April report on credit risk modelling. The organisation of this note largely reflects the organisation of the original report.Overview of Conceptual Approaches to Credit Risk ModellingOn the definition of a credit risk model, respondents noted that no one model type was suitable across all portfolio: techniques used in measuring the credit risk inherent in corporate loan portfolios, trading and derivatives portfolios and in retail portfolios might be different from each other. One response noted that a definition of VaR-style credit risk model (namely one aimed at generating confidence intervals of density functions) was a narrow definition. An eclectic approach to modelling should be permitted, in particular models based on scenarios or other techniques should be seen as acceptable types of model.On the definition of loss, the report discussed two main types of definition: default-mode (DM) and mark-to market (MTM). Responses were divided in their opinion: some argued that both types of definition could lead to acceptable models, and moreover that there should not be an ex-ante preference on the part of the Committee that marking assets to market (or to fair value) in the banking book should be a precondition to using models in setting credit risk capital requirements. Others argued that the MTM approach was a superior definition in that it was the more accurate and sound method for measuring potential losses.On the choice of time horizon, there were no strong views expressed. Respondents felt that the choice of horizon depended on whether the model was intended to measure risk of loss under a liquidation scenario or over a time period over which mitigating action could be taken. One year was felt to be appropriate for the latter by most respondents. One respondent suggested that the choice of time horizon may need to be decided with reference to the financial practices and market conditions prevailing within individual countries.On loss given default, respondents generally agreed that data was a hindrance to accurate estimation, although some felt that there was sufficient data on US and European credits.On the shape of the density function, responses accepted the report’s observation that there were no standard functional forms for the loss distribution and that the tails of distributions were inherently difficult to measure. However, respondents argued that this was not a barrier to successful modelling.On the choice between models that are conditional on the state of the economy or unconditional, a few comments were received on this point. One response felt that credit models needed to be combined with macroeconomic forecasts, given the dependency of credit losses on the economic environment, while others felt that conditional models were appropriate only if there was a sound empirical linkage. One response noted that good risk management practice meant models or their parameters might require adjustment in the event of adverse economic conditions.As a general comment, one respondent noted that more sophisticated modelling techniques did not necessarily result in more successful models; simple models could be useful. Parameter Specification and EstimationThere was agreement that data issues were a significant challenge for modelling activity. Responses drew attention to the fact that data availability was good in some areas, e.g. on US corporate bonds. One respondent pointed out that the legitimate questions concerning data availability should not be taken to mean that data sets used in the parameterisation credit risk models needed to be of similar quality as those used in the market risk arena.Some responses argued that data deficiencies occurred because of the absence of data distribution channels and incentives. Data deficiency could be overcome through the use of data proxies and through the making of conservative assumptions.One response argued that continued development of credit modelling would itself provide a spur to better data collection, both by banks themselves, but also by encouraging data vendors to facilitate bank-to-bank data distribution. A suggestion made by a few respondents was that some sort of data-pooling could ameliorate data deficiencies and one response suggested that a public data warehouse should be introduced, with data supplied by each bank within a given region. One respondent suggested that regulators were well placed to promote data availability, such as producing standardised data definitions.Some international respondents reflected a concern that model parameters might be derived from US data and thus might be insensitive to patterns of credit risk present in regional economies. These respondents gave examples of institutional idiosyncrasies in individual countries or regions that were not well reflected in commercially available credit modelling packages.ValidationRespondents agreed that validation of models was an important factor in moving to their use in regulatory applications. There was also agreement with the Committee’s view that market risk style backtesting did not transfer easily to credit risk models. Such a backtesting standard was seen as neither practical nor methodologically feasible. These respondents pointed out that insufficient data existed to undertake this form of validation; moreover, the length of runs required for a satisfactory test would include historical data that was inappropriate to the modern-day financial industry.Some responses included practical suggestions on validation methods, summarised as follows. One approach suggested by a number of respondents was reliance on sensitivity analysis and stress-testing. It was felt that sensitivity analysis could contribute insight into the model’s performance in response to incremental changes in key parameters and quantitative assumptions; it could demonstrate that model outputs responded in a directionally intuitive fashion in response to changes in inputs and could be a key diagnostic tool in validation work. Stress testing could help demonstrate of a model’s internal consistency, as well as demonstrate the model’s performance under conditions of extreme events (although a limitation was that application of a stress event might take a model outside the range within which it was designed to operate). Respondents agreed that stress testing should form part of sound risk management practice regardless of its use in validating models for regulatory purposes.Another suggested method was the use of test portfolios or the use of panel data or test portfolios (perhaps randomly selected) in addition to broader measures of historical loss experience. One response suggested that supervisors and banks could work together to undertake a series of quantitative exercises to test various credit risk models.Respondents emphasised the need to take account of the context within which models were being used when assessing them, since model construction and operation could vary significantly depending on the portfolio typically held by the bank and the choice of loss definition. One respondent argued that a model was a tool designed to assist the modeller in making estimate of credit risk; on this view, validation work should include not just the model but also the way in which the model is used to measure risk.Some responses discussed methods of internal model validation: these pointed to the need for comprehensive documentation of models and the need for independent review of models within organisations as part of a satisfactory validation process.Supervisory and regulatory application of modelsMost responses acknowledged that the Committee had taken a significant step in looking at models and considering them for use in regulatory capital requirements. It was noted that an advantage would be that, if models were to be used in setting capital requirement, banks would not need to maintain costly dual risk measurement systems for regulatory and internal purposes.A number of responses noted a possible role for the regulatory community in providing guidance for the industry on good practice and minimum standards and in fostering the use of models generally. It was felt that this was important in order to stimulate development of, andimprovements in, risk management as well as to avoid tension caused by differing regulatory and internal measures of credit risk. The Committee was looked to for leadership, co-ordination and information sharing.One response expressed caution over using models in setting capital requirements, arguing that, for institutions with a good history of relationship lending, a model was an inferior tool of comparative risk assessment.Some respondents suggested that models be recognised or approved on a portfolio by portfolio basis. It was claimed that this would be in line with the fact that banks implement models gradually, as they gain confidence in the model and in the data used to support it. In this way, it was argued, banks would have an incentive to implement models across broad portions of their business in a timely manner. If, conversely, regulators required complete coverage of a model before considering it for recognition, then the incentives would be likely to lead to delay in the modelling process and consequently prove counterproductive.On internal use of models, respondents were in strong agreement that models should be used internally for risk management purposes or some other internal application if they were to be considered for use in setting capital requirements. Caution was expressed that this should not result in regulators requiring regulatory models to be used for internal purposes. One respondent argued that the test should be whether models helped to improve the efficiency of business and accuracy of risk analysis; to this end they suggested as a test of internal use that models should fulfil two out of three internal functions: economic capital, concentration/exposure limits and assessing risk-adjusted capital.Several responses picked up on the Committee’s desire that the output of models should be comparable across institutions. Respondents stated that a definition of comparability that required model differences to be “explained”, or related to parameter differences or model differences, was acceptable to the industry. However, if “comparable” meant that the Committee wished to see standardisation or uniformity among models, this was seen as undesirable and unrealistic. Arguments included the need to foster innovation and diversity, the need for competition between financial institutions in risk measurement technique, and the need to avoid requiring banks to use regulatory models for internal purposes. Encouragement of standardisation of model output might foster manipulation of data or model assumptions in a way that reduced the value of regulatory reliance on internal models.Some respondents expressed the hope that, until such time as a full portfolio models approach to credit risk capital requirements was adopted, institutions that continue to invest in credit risk modelling to improve their risk management practices should be provided with incentives in the form of regulatory relief under the proposed supervisory review pillar of the revised capital accord (“Pillar 2”). Regulatory relief should be commensurate with an institution’s level of progress in migrating towards industry best practices. This would incentivise banks to invest in modelling; moreover, by ensuring that active credit risk management practices develop, the availability of data needed to run and parameterise the models would be improved.5。