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RINSE-ADDED FABRIC TREATMENT COMPOSITION

RINSE-ADDED FABRIC TREATMENT COMPOSITION

专利名称:RINSE-ADDED FABRIC TREATMENTCOMPOSITION发明人:PRICE, Kenneth, Nathan,BETTIOL, Jean-Luc, Philippe,BROWN, Nicola, Kay,GREEN, Simon,Richard,LI, Li,O'CONNOR, Helen,Frances,MORINI, Massimo申请号:US2001019830申请日:20010621公开号:WO01/098447P1公开日:20011227专利内容由知识产权出版社提供摘要:A rinse-added fabric treatment composition having a rinse aid increases the rinse capacity of an aqueous rinse bath solution for removing laundry residue from laundered fabrics. When properly diluted in water, the rinse-added fabric treatment composition provides a rinse bath solution having a rinsing capacity of greater than 1. In addition, a rinse-added fabric treatment composition reduces the surfactant residue on a fabric, and includes from about 0.05 % to about 10 % of a residue reduction agent, a suds suppresser, and the balance adjunct ingredients. The residue reduction agent is selected from a cationic residue reduction agent, a zwitterionic residue reduction agent, and a combination thereof. Moreover, methods for reducing surfactant residue on fabric and a method for reducing the amount of water used in a rinsing step of a laundry process are included. A kit for improving the rinsing capacity of water includes a rinse-added fabric treatment composition and an instruction set.申请人:THE PROCTER & GAMBLE COMPANY地址:US国籍:US代理机构:REED, T., David 更多信息请下载全文后查看。

Diva Decloaker 10X Pretreatment Reagent 说明书

Diva Decloaker 10X Pretreatment Reagent 说明书

Intended Use:For In Vitro Diagnostic UseHeat induced antigen retrieval of formalin-fixed paraffin-embedded (FFPE) tissues for immunohistochemistry (IHC) procedures. The clinical interpretation of any staining or its absence should be complimented by morphological studies using proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.Summary & Explanation:Diva Decloaker is a heat retrieval solution that is compatible with virtually all antibodies and eliminates the need for multiple buffers including citrate buffer, EDTA or high pH tris buffers. Antibody titers are doubled and tripled when compared to citrate buffer, pH 6.0. Diva Decloaker incorporates Assure™ tech nology, a color-coded high temperatures pH indicator solution. The end-user is assured by visual inspection that the solution is at the correct dilution and pH. This product is specially formulated for superior pH stability at high temperatures and will help prevent the possibility of losing pH sensitive antigens. Diva Decloaker is non-toxic, non-flammable, odorless and sodium azide and thimerosal free.Known Applications:Immunohistochemistry (formalin-fixed paraffin-embedded tissues) Supplied As:100mlDiva Decloaker, 10X concentrate (DV2004LX)500mlDiva Decloaker, 10X concentrate (DV2004MX)Materials and Reagents (Needed But Not Provided): Microscope slides, positively chargedDesert Chamber* (Drying oven)Positive and negative tissue controlsXylene (Could be substituted with xylene substitute*)Ethanol or reagent alcoholDecloaking Chamber* (Pressure cooker)Deionized or distilled waterWash buffer*(TBS/PBS)Enzyme digestion*Avidin-Biotin Blocking Kit*(Labeled Streptavidin Kits Only) Peroxidase block*Protein block*Primary antibody*Negative control reagents*Detection kits*Detection components*Chromogens*Hematoxylin*Bluing reagent*Mounting medium** Biocare Medical Products: Refer to a Biocare Medical catalog for further information regarding catalog number and ordering information. Certain reagents listed above are based on specific application and detection system used. Storage and Stability:Store at room temperature. Do not use after expiration date printed on vial. If reagents are stored under conditions other than those specified in the package insert, they must be verified by the user. Diluted reagents should be used promptly; any remaining reagent should be stored at room temperature.Protocol Recommendations:1. Deparaffinize tissues and hydrate to water. If necessary, block for endogenous peroxidase and wash in DI water.2. Dilute concentrated Diva Decloaker at a ratio of 1:10 (1 ml Diva to 9 ml of deionized water).3. Place slides into 1X retrieval solution in a slide container (e.g. Coplin Jar, Tissue -Tek™ staining dish or metal slide canister).4. Retrieve sections under pressure using Biocare's Decloaking Chamber. Follow the recommendations on the antibody data sheet and Decloaking Chamber User Manual.5. Check solution for appropriate color change. (See Technical Note #1)6. Gently rinse by gradually adding DI water to the solution, then remove slides and rinse with DI water.Technical Notes:1. Concentrated Diva Decloaker is a bright yellow color. RTU or 1X solution is a pale yellow color. When the solution reaches 80-125°C, the solution turns yellow and indicates that the high temperature solution is at correct pH. Should the pH rise above 7.0, the solution turns a fuschia red color. Should the pH drop too low, thesolution turns a pink color.2. If using Biocare’s Desert Chamber Pro (a programmable turbo-action drying oven), dry sections at 25ºC overnight or at 37ºC for 30-60 minutes and then dry slides at 60ºC for 30 minutes.3. Use positive char ged slides (use Biocare’s Kling-On HIER Slides) and cut tissues at 4-5 microns. Do not use any adhesives in the water bath. Poor fixation and processing of tissues will cause tissue sections to fall off the slides, especially fatty tissues such as breast. Tissues should be fixed a minimum of 6-12 hours.4. Protocol time and temperatures for HIER can vary depending on the Decloaking Chamber model used. Please refer to the relevant Decloaking Chamber manual for appropriate protocol times and temperatures.Limitations:The protocols for a specific application can vary. These include, but are not limited to: fixation, heat-retrieval method, incubation times, tissue section thickness and detection kit used. Due to the superior sensitivity of these unique reagents, the recommended incubation times and titers listed are not applicable to other detection systems, asresults may vary. The data sheet recommendations and protocols are based on exclusive use of Biocare products. Ultimately, it is the responsibility of the investigator to determine optimal conditions. The clinical interpretation of any positive or negative staining should be evaluated within the context of clinical presentation, morphology and other histopathological criteria by a qualified pathologist. The clinical interpretation of any positive or negative staining should be complemented by morphological studies using proper positive and negative internal and external controls as well as other diagnostic tests.Catalog Number: DV2004 LX, MX Description: 100, 500 ml, concentrateQuality Control:Refer to CLSI Quality Standards for Design and Implementation of Immunohistochemistry Assays; Approved Guideline-Second edition (I/LA28-A2). CLSI Wayne, PA, USA (). 2011 Precautions:1. This product is not classified as hazardous. The preservative used in this reagent is Proclin 300 and the concentration is less than 0.25%. Overexposure to Proclin 300 can cause skin and eye irritation and irritation to mucous membranes and upper respiratory tract. The concentration of Proclin 300 in this product does not meet the OSHA criteria for a hazardous substance. Wear disposable gloves when handling reagents.2. Specimens, before and after fixation, and all materials exposed to them should be handled as if capable of transmitting infection and disposed of with proper precautions. Never pipette reagents by mouth and avoid contacting the skin and mucous membranes with reagents and specimens. If reagents or specimens come in contact with sensitive areas, wash with copious amounts of water.3. Microbial contamination of reagents may result in an increase in nonspecific staining.4. Incubation times or temperatures other than those specified may give erroneous results. The user must validate any such change.5. Do not use reagent after the expiration date printed on the vial.6. The SDS is available upon request and is located at /.7. Consult OSHA, federal, state or local regulations for disposal of any toxic substances. Proclin is a trademark of Rohm and Haas Company, or of its subsidiaries or affiliates.Troubleshooting:Follow the antibody specific protocol recommendations according to data sheet provided. If atypical results occur, contact Biocare's Technical Support at 1-800-542-2002.。

iScript_cDNA_Synthesis_Kit

iScript_cDNA_Synthesis_Kit
The maximum amount of the cDNA reaction that is recommended for downstream PCR is one-tenth of the reaction volume, typically 2µl.
*When using larger amounts of input RNA (>1µg) the reaction should be scaled up e.g. 40µl reaction for 2µg, 100µl reaction for 5µg to ensure optimum synthesis efficiency.
The iScript reverse transcriptase is RNAse H+, resulting in greater sensitivity than RNAse H- enzymes. iScript is a modified MMLV-derived reverse transcriptase, optimized for reliable cDNA synthesis over a wide dynamic range of input RNA. The enzyme is provided pre-blended with RNAse inhibitor. The unique blend of oligo (dT) and random hexamer primers in the iScript Reaction Mix works exceptionally well with a wide variety of targets. This blend is optimized for the production of targets < 1kb in length.

超级感受态细菌制备试剂盒

超级感受态细菌制备试剂盒
质粒构建的特殊大肠杆菌也适用,制备出来的感受态菌转化质粒肯定没有问题,但如果转化连接产物有时效果不如DH5α 等其它常见的细菌。 本试剂盒提供了专门用于制备超级感受态的特殊细菌培养液,可以提高制备出来的感受态菌的转化效率,同时也方便了 用户,不必自行配制相应的培养液。 本试剂盒可以分多次使用,共可以制备足够进行100次转化的感受态细菌。
进行。
(2) 4℃4000g离心5分钟收集细菌,弃上清。 注意:离心机必须预先冷却。 (3) 如果离心沉淀前的菌量为50毫升,按后续操作进行,如果是其它体积则按比例换算后进行后续操作。 (4) 用20毫升预冷的超级感受态制备试剂A轻轻重悬细菌沉淀。悬浮时一定要很轻很慢,否则会影响效果。 (5) 冰浴15分钟。 (6) 4℃4000g离心5分钟收集细菌,弃上清。注意:离心机必须预先冷却。 (7) 用2毫升预冷的超级感受态制备试剂B轻轻重悬细菌沉淀。悬浮时一定要很轻很慢,否则会影响效果。 (8) 冰浴15分钟。 (9) 在冰浴上进行分装,可以根据需要适当分装成50-200微升/管。 (10) 立即使用或用液氮或乙醇干冰浴速冻后-70℃保存。通常6个月内效率不会有明显的变化。 6. 感受态细菌的使用: (1) 对于感受态细菌效率的测定或连接产物的转化(其它如基因突变产物的转化等参考连接产物进行): a) 在冰上缓慢融解感受态细菌(对于新鲜制备的感受态就可以直接使用了),如果检测感受态细菌的效率,加入10pg-1ng
钟或更长时间使细菌充分沉淀下来。去除约900-950微升LB培养液。细菌沉淀用余下的LB培养液重悬,然后全部涂 布到含有适当抗生素的LB平板上。37℃培养过夜。 (2) 对于质粒的转化: a) 融解感受态细菌,加入不少于50ng质粒到30-50微升感受态细菌中。质粒的量可以是1微克或更多,但体积不宜超过 感受态细菌量的10%。 b) 轻轻用手指弹动离心管,以混匀细菌和质粒。冰浴或冰水浴放置10分钟。 c) 取全部菌液直接涂布到含有适当抗生素的LB平板上,37℃培养过夜。

化学标准参考手册说明书

化学标准参考手册说明书

3-772CHEM-LABNV-BELGIUM-Tel.+32(0)50288320-Fax+32(0)*****************************.chem-lab.be4CHEM-LABNV-BELGIUM-Tel.+32(0)50288320-Fax+32(0)*****************************.chem-lab.beICP Single Element Standards 10 000 mg/LI C P S TA N D A R D S 10.000 P P MElement HNO 3HCl H 2ONH 4OHHFHNO 3/HF HNO 3/tart.KOHNaOHOthersAl CL01.0103CL01.0104Sb CL01.0123CL01.0124As CL01.0134Ba CL01.0203CL01.0204Be CL01.0214Bi CL01.0223B CL01.0233Cd CL01.0303Ca CL01.0314Ce CL01.0323Cs CL01.0333Cr CL01.0364CL01.0363Co CL01.1123CL01.1128Cu CL01.1133CL01.1134Dy CL01.0433Er CL01.0503Eu CL01.0513Gd CL01.0703Ga CL01.0713Ge CL01.0743Au CL01.0733Hf CL01.0804CL01.0803Ho CL01.0823In CL01.0923Ir CL01.0933Fe CL01.0903CL01.0904La CL01.1203Pb CL01.1223Li CL01.1214Lu CL01.1233Mg CL01.1304CL01.1310Mn CL01.1313Hg CL01.1153Mo CL01.1334CL01.1333Nd CL01.1413Ni CL01.1423Nb CL01.1433Pd CL01.1603P CL01.0643CL01.0633CL01.0634Pt CL01.1613K CL01.1104Pr CL01.1623Re CL01.1804CL01.1803Rh CL01.1813Rb CL01.1824Ru CL01.1834Sm CL01.1903Sc CL01.1913Se CL01.1923Si CL01.1943CL01.1934CL01.1933Ag CL01.2603Na CL01.1404Sr CL01.1963S CL01.2644CL01.2643Ta CL01.2003CL01.2004Te CL01.2015CL01.2014Tb CL01.2023Tl CL01.2033Th CL01.2043Tm CL01.2053Sn CL01.2063Ti CL01.2073CL01.2074W CL01.2303CL01.2304CL01.2333V CL01.2203Yb CL01.2503Y CL01.2513Zn CL01.2613ZrCL01.2633I C P S T A N D A R D SCHEM-LAB NV - BELGIUM - Tel. +32 (0)50 28 83 20 - Fax +32 (0)50 78 26 54 - info@chem-lab.be - www.chem-lab.be6AntimonyArsenicumBariumBerylliumBismuthBoronCadmiumCalciumCalcium oxideCeriumCesiumI C P S I N G L E E L E M E N T S CHEM-LABNV-BELGIUM-Tel.+32(0)50288320-Fax+32(0)*****************************.chem-lab.be7CobaltCopperDysprosiumErbiumEuropiumGadoliniumGalliumGermaniumGoldHafniumHolmium8CHEM-LABNV-BELGIUM-Tel.+32(0)50288320-Fax+32(0)*****************************.chem-lab.beIridiumIronLanthanumLeadLithiumLutetiumMagnesiumNEWMagnesium oxideManganeseMercuryMolybdenumNeodymiumI C P S I N G L E E L E M E N T S CHEM-LABNV-BELGIUM-Tel.+32(0)50288320-Fax+32(0)*****************************.chem-lab.be9NiobiumPalladiumPhosphorusPhosphorus pentoxidePlatinumPotassiumPotassium oxidePraseodymiumRheniumRhodiumRubidiumCHEM-LABNV-BELGIUM-Tel.+32(0)50288320-Fax+32(0)*****************************.chem-lab.be10SamariumScandiumSeleniumSiliciumSilverSodiumSodium oxideStrontiumSulfurTantalumTelluriumI C P S I N G L E E L E M E N T S CHEM-LABNV-BELGIUM-Tel.+32(0)50288320-Fax+32(0)*****************************.chem-lab.be11ThalliumThoriumThuliumTinTitaniumTungstenVanadiumYtterbiumYttriumZincZirconiumCHEM-LABNV-BELGIUM-Tel.+32(0)50288320-Fax+32(0)*****************************.chem-lab.be12A Certificate of Analysis is provided with each ICP standard stating:- Actual certified concentration of the final solution- Traceability to NIST- Expiration date- Trace impurities detected I C P S I NCHEM-LABNV-BELGIUM-Tel.+32(0)50288320-Fax+32(0)*****************************.chem-lab.be13ICP Single Element Standards 1 000 mg/LI C P S TA N D A R D S 1.000 P P MElement HNO 3HCl H 2ONH 4OHHFHNO 3/HF HNO 3/tart.KOH NaOHOthersAl CL01.0101CL01.0102Sb CL01.0121CL01.0122CL01.0162As CL01.0133CL01.0132CL01.0131Ba CL01.0201CL01.0202Be CL01.0212CL01.0211Bi CL01.0221B CL01.0232CL01.0231Cd CL01.0301Ca CL01.0311CL01.0312Ce CL01.0321Cs CL01.0331Cr CL01.0362CL01.0361CL01.0352Co CL01.1121CL01.1122Cu CL01.1131CL01.1132Dy CL01.0431Er CL01.0501Eu CL01.0511Gd CL01.0701Ga CL01.0711Ge CL01.0741CL01.0721Au CL01.0731Hf CL01.0802CL01.0801Ho CL01.0821In CL01.0921Ir CL01.0931Fe CL01.0901CL01.0902La CL01.1201CL01.1202Pb CL01.1221Li CL01.1212CL01.1211Lu CL01.1231Mg CL01.1301CL01.1302Mn CL01.1311CL01.1312Hg CL01.1151Mo CL01.1332CL01.1331Nd CL01.1411Ni CL01.1421CL01.1422Nb CL01.1431Os CL01.1501Pd CL01.1601P CL01.0641CL01.0631Pt CL01.1611K CL01.1101CL01.1102Pr CL01.1621Re CL01.1802CL01.1801Rh CL01.1811Rb CL01.1822CL01.1821Ru CL01.1831Sm CL01.1901Sc CL01.1911Se CL01.1922CL01.1921Si CL01.1999CL01.1945CL01.1932CL01.1931CL01.1935Ag CL01.2601Na CL01.1401CL01.1402Sr CL01.1962CL01.1961S CL01.2641CL01.2642Ta CL01.2001CL01.2002Te CL01.2012CL01.2013CL01.2011Tb CL01.2022Tl Th CL01.2041Tm CL01.2051Sn CL01.2061CL01.2062Ti CL01.2072CL01.4601CL01.2071CL01.2075W CL01.2302CL01.2301CL01.2331V CL01.2201Yb CL01.2501Y CL01.2511Zn CL01.2611CL01.2612ZrCL01.2632CL01.2631CL01.2672I C P S I N G L E E L E M E N T S T A N D A R D SCHEM-LAB NV - BELGIUM - Tel. +32 (0)50 28 83 20 - Fax +32 (0)50 78 26 54 - info@chem-lab.be - www.chem-lab.be14Aluminium(III) oxideAntimonyArsenicumBariumBerylliumBismuthBoronCadmiumI C P S I N G L E E L E M E N T S CHEM-LABNV-BELGIUM-Tel.+32(0)50288320-Fax+32(0)*****************************.chem-lab.be15Calcium oxideCeriumCesiumChromiumCobaltCopperDysprosiumErbiumEuropiumGadoliniumGalliumCHEM-LABNV-BELGIUM-Tel.+32(0)50288320-Fax+32(0)*****************************.chem-lab.be16GoldHafniumHolmiumIndiumIridiumIronIron(III) oxideLanthanumLeadLithiumLutetiumI C P S I N G L E E L E M E N T S CHEM-LABNV-BELGIUM-Tel.+32(0)50288320-Fax+32(0)*****************************.chem-lab.be17Magnesium oxideManganeseManganese(III) oxideMercuryMolybdenumNeodymiumNickelNiobiumOsmiumPalladiumDon’t see the exact solution you need?E-mail us the Tailor Made Standard Quotation request form in the back of the catalog. 18CHEM-LABNV-BELGIUM-Tel.+32(0)50288320-Fax+32(0)*****************************.chem-lab.bePhosphorus pentoxidePlatinumPotassiumPotassium oxidePraseodymiumRheniumRhodiumRubidiumRutheniumSamariumScandiumI C P S I N G L E E L E M E N T S CHEM-LABNV-BELGIUM-Tel.+32(0)50288320-Fax+32(0)*****************************.chem-lab.be19SiliciumSilicium dioxideSilverSodiumSodium oxideStrontiumSulfurTantalumCHEM-LABNV-BELGIUM-Tel.+32(0)50288320-Fax+32(0)*****************************.chem-lab.be20TerbiumThalliumThoriumThuliumTinTitaniumTungstenVanadiumYtterbiumI C P S I N G L E E L E M E N T SZincZirconiumLuis BianchiISO/IEC 17025:2005 - General requirements for the competence of calibration laboratories ISO 9001:2008 - Quality ManagementISO Guide 34:2009 - General requirements for the competence of reference material producers A Certificate of Analysis is provided with each ICP standard stating:- Actual certified concentration of the final solution - Traceability to NISTAntimonyArsenicumBariumBerylliumBoronCadmiumCalciumCeriumCesiumChromiumCobaltCopperDysprosiumErbiumI C P S I N G L E E L E M E N T SGadoliniumGalliumGermaniumGoldHafniumHolmiumIndiumIridiumIronLanthanumLeadLithiumLutetiumManganeseMercuryMolybdenumNeodymiumNickelNiobiumOsmiumPalladiumPhosphorusPlatinumPotassiumPraseodymiumRheniumRhodiumI C P S I N G L E E L E M E N T SRutheniumSamariumScandiumSeleniumSiliciumSilverSodiumSulfurTantalumTelluriumTerbiumThalliumThoriumThuliumTinTitaniumTungstenVanadiumYtterbiumYttriumZincZirconium NEWDon’t see the exact solution you need?I C P S I N G L E E L E M E N T SAluminiumAntimonyBariumBerylliumBismuthBoronCadmiumCalciumCeriumCesiumChromiumCobaltCopperDysprosiumEuropiumGadoliniumGalliumGermaniumGoldHafniumHolmiumIndiumIridiumIronLanthanumLeadI C P S I N G L E E L E M E N T SLutetiumMagnesiumManganeseMercury NEWMolybdenumNeodymiumNickelNiobiumOsmiumPalladiumPhosphorusPlatinumPotassiumPraseodymiumRhodiumRubidiumRutheniumSamariumScandiumSeleniumSiliciumSilverSodiumSulfurTantalumTelluriumTerbiumThalliumI C P S I N G L E E L E M E N T SThuliumTinTitaniumTungstenVanadiumYtterbiumYttriumZincZirconium NEWChem-Lab’s certified “Custom Made Standards” will save you time and money.Multi Element ICP QC Standard sol. (QCS-23) (23E)CL01.13610Multi Element ICP QC Standard sol. (QCS-01) (23E)CL01.13601I C P M U L T I E L E MMulti Element ICP QC Standard sol. (QCS-04) (19E)NEW CL01.13604Multi Element ICP QC Standard sol. (QCS-19) (19E)CL01.13608Multi Element ICP QC Standard sol. (QCS-02) (7E)CL01.13602Multi Element ICP ASL QC Standard sol. (QCS-ASL-7) (7E)CL01.13607Multi Element ICP QC Standard sol. (QCS-06) (4E)CL01.13606I C P M U L T I E L E MA Certificate of Analysis is provided with each ICP standard stating:- Actual certified concentration of the final solution- Traceability to NIST- Expiration date- Trace impurities detectedMulti Element ICP SQS Standard sol. (SQS-01) (33E)CL01.13631I C P M U L T I E L E MReference Materials - Contents of certificates and labelsGeneral requirements for the competence of calibration laboratoriesPage 2 of 2Chem-Lab multi-element standards are compared against the following NIST SRMs Element Aq. SRMOil SRMElement Aq. SRMOil SRMAg 31511077a Nb 3137-Al 3101a 1075a Nd 3135a -As 3103a 3103a Ni 31361065b Au 3121-NO3-3185-B 31073107NO2-136e -Ba 3104a 1051b P 3139a 1071b Be 3105a 3105a Pb 31281059c Bi 31063106Pd 3138-Br-3184-PO4-33186-Ca 3109a 3109Pr 3142a -Cd 31081053a Pt 3140-Ce 3110-Rb 3145a -Cl-919b -Re 3143-Co 31133113Rh 3144-Cr 3112a 1078b S 31543154Cs 3111a -Sb 3102a 3102a Cu 31141080a Sc 3148a 3148a Dy 3115a -Se 31493149Er 3116a -Si 31501066a Eu 3117a -Sm 3147a -F-3183-Sn 3161a 1057b Fe 3126a 1079b SO4-23181-Ga 3119a -Sr 3153a 1070a Gd 3118a -Ta 3155-Ge 3120a -Tb 3157a -Hf 3122-Te 3156-Hg 31333133Th 3159-Ho 3123a -Ti 3162a 3162a In 3124a -Tl 31583158K 3141a 3141a Tm 3160a -La 3127a 3127a U 3164-Li 3129a 1060a V 31651052b Lu 3130a -W 3163-Mg 3131a 3131a Y 3167a 3167a Mn 31323132Tb 3166a -Mo 31343134Zn 3168a 1073b Na3152a1069bZr31693169*ICP-EPA Methods (Method 200.7 Version 3.3 & earlier) - Laboratory Performance Check Standard (LPCS) Contains 29 elements in 5% HNO3Multi Element ICP LFSS Standard sol. LFSS-01 (25E)CL01.13772*ICP-EPA Methods (Method 200.7 Version 3.3 & earlier) - Laboratory Fortifying Stock Solution (LFSS) Contains 25 elements in 5% HNO3 + traces HFMulti Element ICP SP Standard sol. SP-03 (12E)CL01.13743I C P M U L T I E L E*ICP-EPA Methods (Methods 6010A - 6010B - 200.7 Version 3.3 and earlier) - Mixed Calibration Standard 1 Contains 6 elements in 2% HNO3 (MCS-Multi Element ICP CAL Standard sol. MCS-04 (6E)CL01.13734Multi Element ICP SP Standard sol. SP-05 (5E)CL01.13745Multi Element ICP CAL Standard sol. MCS-02 (5E)CL01.13732 *ICP-EPA Methods (Methods 6010A - 6010B - 200.7 Version 3.3 and earlier) - Mixed Calibration Standard 2 Contains 5 elements in 2% HNO3 (MCS-Multi Element ICP SP Standard sol. SP-05R (5E)CL01.13754*ICP-EPA Methods (Method 200.7 Version 3.3 and earlier) - Spiking Standard for Drinking Water # 5R Contains 5 elements in 5% HNO3 (M-200.7-SP-Multi Element ICP SIC Standard sol. SICS-02 (5E)CL01.13762Multi Element ICP PLASOL Standard sol. M-200.7-PLASOL-1 (4E)CL01.13723*ICP-EPA Methods (Method 200.7 Version 4.4, May 1994) - Plasma Solution (PLASOL) - Determining optimum viewing height of the plasma analytical zone.I C P M U L T I E L E MMulti Element ICP SP Standard sol. SP-01R (4E)CL01.13751 *ICP-EPA Methods (Method 200.7 Version 3.3 and earlier) - Spiking Standard for Drinking Water # 1R Contains 4 elements in H2O + traces HF (M-Multi Element ICP SP Standard sol. SP-02R (4E)CL01.13752 *ICP-EPA Methods (Method 200.7 Version 3.3 and earlier) - Spiking Standard for Drinking Water # 2R Contains 4 elements in 2% HNO3 (M-200.7-SP-Multi Element ICP CAL Standard sol. MCS-03 (3E)CL01.13733Tailor Made Mixtures can be formulated to meet your special applications.Multi Element ICP SP Standard sol. SP-01 (3E)CL01.13741Multi Element ICP TUNSOL Standard sol. M-200.7-TUNSOL-1 (2E)CL01.13724*ICP-EPA Methods (Method 200.7 Version 4.4, May 1994) - Tuning Solution (TUNSOL) - Adjusting the aerosol argon gas flow prior to calibration and analysis.Mono Element ICP SP Standard sol. TCLP-02 (1E)CL01.13773*ICP-EPA Methods (Methods 6010B - 200.7 Version 3.3 and earlier) - Spiking & Mercury Standard - TCLP Standaard 2 Contains 1 elements in 5%I C P M U L T I E L E MMono Element ICP SP Standard sol. SP-04R (1E)CL01.13753 *ICP-EPA Methods (Method 200.7 Version 3.3 and earlier) - Spiking Standard for Drinking Water # 4R Contains 1 elements in 2% HNO3 (M-200.7-SP-Mono Element ICP SIC Standard sol. SICS-01 (1E)CL01.13761Mono Element ICP SP Standard sol. TCLP-02-10X (1E)CL01.13746*ICP-EPA Methods (Method 200.7 Version 4.4, May 1994) - Instrument Fortifying (Spiking) Standard # 1 Contains 26 elements in 5% HNO3 + tracesMulti Element ICP IPC Standard sol. M-200.7-IPC-01 (26E)CL01.13721 *ICP-EPA Methods (Method 200.7 Version 4.4, May 1994) - Instrument Performance Check (IPC) Contains 26 elements in 5% HNO3 (M-200.7-IPC-I C P M U L T I E L E*ICP-EPA Methods (Method 200.7 Version 4.4, May 1994) - Instrument Fortifying (Spiking) Standard for Solids # 1 Contains 24 elements in 5% HNO3Multi Element ICP LFSS Standard sol. M-200.7-LFSS-01W (22E)CL01.13712 *ICP-EPA Methods (Method 200.7 Version 4.4, May 1994) - Instrument Fortifying (Spiking) Standard for Water # 1 Contains 22 elements in 5% HNO3Multi Element ICP CAL Standard sol. M-200.7-01 (10E)CL01.13701Chem-Lab’s certified “Custom Made Standards” will save you time and money.Multi Element ICP CAL Standard sol. M-200.7-02R (6E)CL01.13702Multi Element ICP LFSS Standard sol. M-200.7-LFSS-02 (5E)CL01.13714*ICP-EPA Methods (Method 200.7 Version 4.4, May 1994) - Instrument Fortifying (Spiking) Standard # 2 Contains 5 elements in 5% HNO3 + traces HFMulti Element ICP IPC Standard sol. M-200.7-IPC-02 (5E)CL01.13722*ICP-EPA Methods (Method 200.7 Version 4.4, May 1994) - Instrument Performance Check (IPC) Contains 5 elements in 5% HNO3 + traces HF (M-I C P M U L T I E L E MMulti Element ICP PLASOL Standard sol. M-200.7-PLASOL-1 (4E)CL01.13723 *ICP-EPA Methods (Method 200.7 Version 4.4, May 1994) - Plasma Solution (PLASOL) - Determining optimum viewing height of the plasma analytical zone.Multi Element ICP CAL Standard sol. M-200.7-03R (4E)CL01.13703Multi Element ICP TUNSOL Standard sol. M-200.7-TUNSOL-1 (2E)CL01.13724 *ICP-EPA Methods (Method 200.7 Version 4.4, May 1994) - Tuning Solution (TUNSOL) - Adjusting the aerosol argon gas flow prior to calibration and analysis.Multi Element ICP QC Standard sol. (QCS-01) (23E)CL01.13601Multi Element ICP INT Standard sol. INT-B1 (12E)CL01.13682I C P M U L T I E L E MMulti Element ICP QC Standard sol. (QCS-02) (7E)CL01.13602Multi Element ICP CAL Standard sol. MCS-01 (6E)CL01.13731 *ICP-EPA Methods (Methods 6010A - 6010B - 200.7 Version 3.3 and earlier) - Mixed Calibration Standard 1 Contains 6 elements in 2% HNO3 (MCS-Multi Element ICP Standard sol. PLASOL-R (5E)CL01.13822A Certificate of Analysis is provided with each ICP standard stating:- Actual certified concentration of the final solution- Traceability to NIST- Expiration date- Trace impurities detected。

ASTM D 1002

ASTM D 1002

B36/B36M Specification for Brass Plate,Sheet,Strip,and Rolled Bar3B152Specification for Copper Sheet,Strip,Plate,and Rolled Bar3B209Specification for Aluminum and Aluminum-Alloy Sheet and Plate4B265Specification for Titanium and Titanium Alloy Strip, Sheet,and Plate5D907Terminology of Adhesives6D4896Guide for Use of Adhesive-Bonded Single Lap-Joint Specimen Test Results6E4Practices for Force Verification of Testing Machines7 3.Terminology3.1Definitions—Many terms in this test method are defined in Terminology D907.4.Significance and Use4.1This test method is primarily comparative.However,it does have application as a discriminator in determining varia-tions in adherend surface preparation parameters and adhesive environmental durability.The test method has found applica-tions in controlling surface preparations,primer,and adhesive systems for determining strength properties of tested systems.4.2The misuse of strength values obtained from this test method as design-allowable stress values for structural joints could lead to product failure,property damage,and human injury.The apparent shear strength of an adhesive obtained from a given small single-lap specimen may differ from that obtained from a joint made with different adherends or by a different bonding process.The normal variation of temperature and moisture in the service environment causes the adherends and the adhesive to swell or shrink.The adherends and adhesive are likely to have different thermal and moisture coefficients of expansion.4.3Even in small specimens,short-term environmental changes may induce internal stresses or chemical changes in the adhesive that permanently affect the apparent strength and other mechanical properties of the adhesive.The problem of predicting joint behavior in a changing environment is even more difficult if a different type of adherend is used in a larger structural joint than was used in the small specimen.4.4The apparent shear strength measured with a single-lap specimen is not suitable for determining design-allowable stresses for designing structural joints that differ in any manner from the joints tested without thorough analysis and under-standing of the joint and adhesive behaviors.4.5Single-lap tests may be used for comparing and select-ing adhesives or bonding processes for susceptibility to fatigue and environmental changes,but such comparisons must be made with great caution since different adhesives may respond differently in different joints.See Guide D4896for further discussion of the concepts relative to interpretation of adhesive-bonded single-lap-joints.5.Apparatus5.1The testing machine shall conform to the requirements of Practices E4.The testing machine shall be so selected that the breaking load of the specimens falls between15and85 percent of the full-scale capacity.The machine shall be capable of maintaining a rate of loading of80to100kg/cm2(1200to 1400psi)/min,or,if the rate is dependent on crosshead motion, the machine should be set to approach this rate of loading, approximately0.05in./min.It shall be provided with a suitable pair of self-aligning grips to hold the specimen.It is recom-mended that the jaws of these grips shall engage the outer25 mm(1in.)of each end of the test specimenfirmly.5.2The grips and attachments shall be so constructed that they will move into alignment with the test specimen as soon as the load is applied,so that the long axis of the test specimen will coincide with the direction of the applied pull through the center line of the grip assembly.5.3The length of overlap of the specimen may be varied where necessary.The length of the specimen in the jaws, however,must not be varied.The distance from the end of the lap to the end of the jaws should be63mm(21⁄2in.)in all tests.6.Test Specimens6.1Test specimens shall conform to the form and dimen-sions shown in Fig.1.These shall be cut from test panels prepared as prescribed in Section7.The recommended thick-ness of the sheets is1.6260.125mm(0.06460.005in.).The recommended length ofoverlap for most metals of1.62mm(0.064in.)in thickness is12.760.25mm(0.560.01in.).6.2Since it is undesirable to exceed the yield point of themetal in tension during test,the permissible length of overlapin the specimen will vary with the thickness and type of metal,and on the general level of strength of the adhesive beinginvestigated.The maximum permissible length may be com-puted from the following relationship:L5Fty t/t(1)where:L=length of overlap,in.,t=thickness of metal,in.,Fty=yield point of metal(or the stress at proportionallimit),psi,andt=150percent of the estimated average shear strengthin adhesive bond,psi.6.3A variation in thickness of the metal,and the length ofoverlap,will likely influence the test values and make directcomparison of data questionable.For this reason,in compara-tive or specification tests,the thickness should preferably be1.6260.125mm(0.06460.005in.)and the length of overlap3Annual Book of ASTM Standards,V ol02.01.4Annual Book of ASTM Standards,V ol02.02.5Annual Book of ASTM Standards,V ol02.04.6Annual Book of ASTM Standards,V ol15.06.7Annual Book of ASTM Standards,V ol03.01.FIG.1Form and Dimensions of Test Specimenshould preferably be12.760.25mm(0.560.01in.),or not in excess of the value computed in6.2.For development tests values could be different,but should then be constant.6.4The following grades of metal are recommended for the test specimens:Metal ASTM DesignationBrass B36,C26800(Alloy8)Copper B152,C11000Aluminum B209,Alloy2024,T3temperSteel A109,Grade2Corrosion-resisting steel A167,Type302Titanium B2656.5At least30specimens shall be tested,representing at least four different joints.However,if statistical analysis of data and variance is employed,it should be possible to reduce this number.7.Preparation of Test Joints7.1It is recommended that test specimens be made up in multiples of at leastfive specimens,and then cut into indi-vidual test specimens(Note1),Fig.2and Fig.3.Cut sheets of the metals prescribed in6.1and6.4to suitable size.All edges of the metal panels and specimens which will be within(or which will bound)the lap joints shall be machined true (without burrs or bevels and at right angles to faces)and smooth(rms160max)before the panels are surface-treated and bonded.Clean and dry the sheets carefully,according to the procedure prescribed by the manufacturer of the adhesive, and assemble in pairs.Prepare and apply the adhesive accord-ing to the recommendations of the manufacturer of the adhe-sive.Apply the adhesive to a sufficient length in the areaacross FIG.2Standard TestPanelthe end of one or both metal sheets so that the adhesive willcover a space approximately 6mm (1⁄4in.)longer than theoverlap as selected in Section 6.Assemble the sheets so thatthey will be held rigidly so that the length of the overlap willbe controlled,as indicated in Section 6,within 0.25mm(60.01in.),and the adhesive allowed to cure as prescribed bythe manufacturer of the adhesive.N OTE 1—Bonding specimens in multiple panels is believed to givemore representative specimens.However,individual specimens may beprepared if agreeable to the supplier or the purchaser of the adhesive.8.Preparation of Test Specimens8.1Cut the test specimens,as shown in Fig.1,from thepanels,Figs.2and 3.Perform the cutting operation so as toavoid overheating or mechanical damage to the joints (Note 2).For final preparation trim panel area according to Fig. 2.Measure the width of the specimen and the length of theoverlap to the nearest 0.25mm (0.01in.)to determine the sheararea.N OTE 2—A five-tooth,typesetter’s circular saw has been found suitablefor such purposes.9.Procedure9.1Test the specimens,prepared as prescribed in Section 8,as soon after preparation as possible.The manufacturer of the adhesive may,however,prescribe a definite period of condi-tioning under specific conditions before testing.9.2Place the specimens in the grips of the testing machine so that the outer 25mm (1in.)of each end are in contact with the jaws (see 5.3)and so that the long axis of the test specimen coincides with the direction of applied pull through the center line of the grip assembly.Apply the loading immediately to the specimen at the rate of 80to 100kg/cm 2(1200to 1400psi)of the shear area per min.Continue the load to failure.This rate of loading will be approximated by a free crosshead speed of 1.3mm (0.05in.)/min.10.Calculations 10.1Record the load at failure and the nature and amount of this failure (cohesion in adhesive or metal,or adhesion)for each specimen.Express all failing loads in kilograms per square centimeter (pounds per square inch)of shear area,calculated to the nearest 0.06cm 2(0.01in.2).11.Report 11.1Report the following:11.1.1Complete identification of the adhesive tested,in-cluding type,source,date manufactured,manufacturers’code numbers,form,etc.,11.1.2Complete identification of the metal used,its thick-ness,and the method of cleaning and preparing its surfaces prior to bonding,11.1.3Application andbonding conditions used in prepar-ing specimens,11.1.4Average thickness of adhesive layer after formation of the joint within 0.001in.(0.025mm).The method of obtaining the thickness of the adhesive layer shall be described including procedure,location of measurements,and range of measurements.11.1.5Length of overlap used,11.1.6Conditioning procedure used for specimens prior to testing,11.1.7Number of specimens tested,11.1.8Number of joints represented and type of joint if other than single overlap,11.1.9Maximum,minimum,and average values for the failing load,and 11.1.10The nature of the failure,including the average estimated percentages of failure in the cohesion of the adhe-sive,contact failure,and adhesion to the metal.12.Precision and Bias 12.1The precision and bias statement for this test method has not been determined yet.Archival and round-robin infor-mation is being reviewed,and the results are expected by September 2004.13.Keywords 13.1adhesives;metal-to-metal;shear strength;single-lap joint;tension loadingFIG.3Optional Panel for Acceptance Tests OnlyThe American Society for Testing and Materials takes no position respecting the validity of any patent rights asserted in connection with any item mentioned in this ers of this standard are expressly advised that determination of the validity of any such patent rights,and the risk of infringement of such rights,are entirely their own responsibility.This standard is subject to revision at any time by the responsible technical committee and must be reviewed everyfive years and if not revised,either reapproved or withdrawn.Your comments are invited either for revision of this standard or for additional standards and should be addressed to ASTM Headquarters.Your comments will receive careful consideration at a meeting of the responsible technical committee,which you may attend.If you feel that your comments have not received a fair hearing you should make your views known to the ASTM Committee on Standards,at the address shown below.This standard is copyrighted by ASTM,100Barr Harbor Drive,PO Box C700,West Conshohocken,PA19428-2959,United States. Individual reprints(single or multiple copies)of this standard may be obtained by contacting ASTM at the above address or at 610-832-9585(phone),610-832-9555(fax),or service@(e-mail);or through the ASTM website().。

科立得酶底物法试剂

科立得酶底物法试剂

科立得酶底物法试剂英文回答:The Coomassie Brilliant Blue G-250 (CBB G-250) dye is commonly used as a substrate in the Coomassie Brilliant Blue (CBB) method for protein quantification. This methodis also known as the Bradford assay. The CBB G-250 dye is a cationic dye that binds to proteins through electrostatic interactions. When proteins are present in a solution, they bind to the CBB G-250 dye, causing a shift in the dye's absorbance spectrum. The change in absorbance can be measured using a spectrophotometer, and the protein concentration can be determined based on a standard curve.The CBB G-250 dye is available as a ready-to-use reagent in the form of a solution. To perform the assay, a small amount of the protein sample is mixed with the CBB G-250 dye solution. After a brief incubation period, the absorbance at a specific wavelength (usually 595 nm) is measured. The absorbance is then compared to a standardcurve generated using known concentrations of a protein standard. The protein concentration in the sample can be determined by extrapolating from the standard curve.The Coomassie Brilliant Blue method is widely used in protein quantification due to its simplicity, sensitivity, and compatibility with a wide range of protein samples. It is commonly used in research laboratories, biotechnology companies, and academic institutions. The assay is relatively quick and can be performed with minimal equipment, making it a convenient choice for routine protein quantification.中文回答:科立得酶底物法试剂是一种常用于蛋白质定量的染料。

达科为 细胞因子套装(CIK专用)说明书

达科为 细胞因子套装(CIK专用)说明书

深圳市达科为生物工程有限公司 地址:深圳市南山区桃源街道光前工业区20栋4C 邮编:518055 Tel: 0755-******** Fax: 0755-******** Web: 细胞因子套装(CIK 专用)说明书CIK Activated Agent (CIK-CYT )DKW35-CYT-CIK001产品描述:细胞因子套装(CIK 专用)是本公司在对CIK 和T 淋巴细胞培养进行大量摸索和实验的基础上,根据优选出的细胞培养所需要的刺激因子的种类及剂量配制而成,主要作为人CIK 细胞培养的诱导活化剂。

本产品批间质量稳定,可搭配各种无血清或有血清培养基用于诱导活化CIK 细胞,使用时按照1:100的比例加入培养基。

包装规格:使用方法:培养细胞时,将组分I (CIK Activated Agent I )和组分II (CIK Activated Agent II )分别按照1:100的比例加入总体系中使用。

培养基总体积组分I组分II 10 mL 100 μL 100 μL 20 mL 200 μL 200 μL 50 mL500 μL500 μL具体培养方法:1. 采血并分离PBMCs 。

2. 使用完全培养基将分离获得的PBMCs 按2-2.5×106个/ml 的浓度接种于T75或T175细胞培养瓶中,按照1:100的比例加入CIK Activated Agent I ,37℃,5%CO 2培养箱中培养。

3. 24hr 后,即第1天按照1:100的比例加入CIK Activated Agent II ,刺激货号名称组分规格 DKW35-CYT-CIK001细胞因子套装(CIK 专用) CIK Activated Agent KitCIK Activated Agent I 500μL CIK Activated Agent II500μL深圳市达科为生物工程有限公司 地址:深圳市南山区桃源街道光前工业区20栋4C 邮编:518055 Tel: 0755-******** Fax: 0755-******** Web: E-mail:*************CIK 细胞的生长和增殖。

Luc-Pair

Luc-Pair

Luc-Pair™Duo-Luciferase HS Assay Kit-高灵敏性双荧光素酶检测试剂盒Cat.No.LF004(100reactions)Cat.No.LF005(300reactions)Cat.No.LF006(1000reactions)使用说明书GeneCopoeia,Inc.广州易锦生物技术有限公司9620Medical Center Drive,#101地址:广州科学城揽月路3号F区F801(510663)Rockville,MD20850电话:4006-020-200、************、************ USA网站:301-762-0888866-360-9531***********************©2016GeneCopoeia,Inc.使用说明书Luc-Pair™Duo-Luciferase HS Assay KitI.产品概述II.产品信息及储存条件III.细胞裂解IV.FLuc和RLuc工作液的配制V.荧光素酶检测流程VI.有限使用许可及质保声明I.产品概述对报告基因表达的转录调控的研究常被应用于生物学研究和药物发现。

荧光素酶在基因表达研究中应用最为广泛,其主要包含以下几个优点:1)在广泛动态范围内具有高灵敏度2)在哺乳动物细胞内无荧光素酶、背景极低3)实验重复性好4)成本低5)操作简单萤火虫和海肾荧光素酶都具有快捷、简便、灵敏度高的检测特点,被公认为是理想的报告基因,因为它们具有完全不同的进化起源、酶学结构和底物要求。

萤光素酶报告基因的测定需要用光度计或多功能微孔板检测仪,且发光强度与荧光素酶的数量成正比。

萤火虫荧光素酶(Photinus pyralis)已被证实是检测启动子活性和监测基因转录后调控状态的理想的报告基因。

它是在细胞质中作用的酶,分子量为61kDa并催化下列反应:海肾(Renilla reniformis)荧光素酶是一个36kDa单亚基蛋白质,酶活性不需要翻译后修饰,因此它可以作为一个实时转录报告基因,催化下面的生物发光反应:此体系可以在目的基因的附近监控顺式作用元件的转录激活。

EPI-REZ

EPI-REZ

Technical Data SheetEPI-REZ™ Resin WD-510Product DescriptionEPI-REZ™ Resin WD-510 is a liquid epoxy resin specifically designed for water dilution. In combination with epoxy curing agents and dilution with water it forms resin in water emulsions. It is useful as a modifier for Portland Cement, concrete, mortars, stuccos, and grouts. As a binder it is useful for trowel applied floor toppings, bond coats, base coats, membranecoatings and sealers.Suggested UsesEPI-REZ WD-510 forms uniform dispersions in water in combination with Aliphatic amine curing agents, including adducts, amidoamines, polyamides, and accelerated amines. To make these dispersions the EPI-REZ WD-510 and curing agent must first be thoroughly blended. Then water is added to the blend and stirred in, either manually or with an agitator powered by a motor. Resin/curing agent blends are diluted with water to a solids level of 40 to 80 percent by weight. This dispersion process is typically facilitated with glycol ether cosolvents and epoxy viscosity reducing modifiers. While batches of the water dispersed system exhibit long dispersion life (generally remain emulsified through gelation), the usable working life of some formulations is shorter than the dispersion life. The actual usable working life of a specific formulation should be determined in a laboratory by studying performance related properties as a function of time elapsed from mixing to application. EPI-REZ WD-510 can also be combined with acid-functional acrylic epoxy resin (e.g. ETERSOL G1182 - Eternal Chemical Co) to produce two-package water reducible coatings. EPI-REZ WD-510 may be used in place of EPON Resin 828 for lower VOC's in combination with waterborne EPIKURE™ Epoxy Curing Agents for 2 package epoxy industrial finishes.Sales SpecificationsTest M ethodColor 2Gardner A STM D1259V iscosity 8000 - 12000cP A STM D2196Weight per Epoxide 190 - 205g/eq A STM D1652Typical PropertiesUnitPounds/Gallon 9.64lbs/galProcessing/How to useGeneral InformationEPI-REZ Resin WD-510 can be thinned with epoxy diluent modifiers including HELOXY™ Modifier 8, HELOXY Modifier 62 or CARDURA™ E-10 to facilitate mixing and handling. Ethylene glycol monopropyl ether may be used to lower viscosity of the resins during the water dispersing process. EPI-REZ Resin WD-510 does not contain alkylphenol ethoxylates.Table 1 outlines the handling characteristics of water reducible systems incorporating EPI-REZ WD-510 with various amine curing agents. Substitution of up to 20 percent of EPI-REZ WD-510 with a flexibilizer or reactive diluent retains the water reducible feature in combination with amine curing agents. Flatting agents such as colloidal silica may be incorporated at levels up to 15 parts per hundred resin in the emulsions without building excessive thixotropy.Cured state properties of EPI-REZ WD-510 amine systems are very similar to those of corresponding EPON™ Resin 828 compositions. Table 2 compares such systems prior to water reduction of the EPI-REZ WD-510 system.Table 1/ Handling Characteristics and Film Properties of Water-Thinned Epoxy SystemsEPI-REZ Resin WD-510100100100100 EPIKURE™ Curing Agent 327438–––EPIKURE Curing Agent 3046–48––EPIKURE Curing Agent 3270––72–EPIKURE Curing Agent 3072–––33 Water53576753 Colloidal Silica11211 Blend Properties at 25 °C280.55 Gel Time, 100 g mass hrs9Emulsion Life72727272 Solids%Film Properties3Tack-Free Time hrs1624811 Film Continuity Good Craters Good Good Clarity Transparent Cloudy Cloudy Cloudy "Sweat-out"None Slight None Slight1 Cab-O Sil M-5 supplied Cabot Corporation.2 Emulsions prepared by first blending the resins, converter and colloidal silica, then adding water and agitating for 2 minutes using a portable electric drill and "Jiffy" agitator.3 5 mil wet films applied with doctor blade at 25 °CTable 2/ Comparison of Cured State Properties of Undiluted EPI-REZ™ Resin WD-510 versus EPON Resin 828EPI-REZ WD-510 Resin100–100–EPON Resin 828–100–100 EPIKURE Curing Agent 32743840––EPIKURE Curing Agent 3072––3335Cured State Properties1Ultimate Tensile Strength psi8,4009,2009,4007,000 Tensile Elongation%344 1.6 Ultimate Flexural Strength psi12,900–14,50015,000 Flexural Modulus106 psi0.41–0.460.52 Compressive Yield Strength psi13,000–12,60013,200 Izod Impact ft.•lb./in. notch0.490.530.520.45 Hardness Shore D8*******Chemical Resistance2Distilled Water0.380.210.200.105% Acetic Acid0.590.250.81–50:50 Xylene/lsopropanol11.68 1.25–1 All test specimens were cured for2 weeks at 25 °C.2 Percent weight gain after immersion for 24 hours at 25 °C.Safety, Storage & HandlingPlease refer to the SDS for the most current Safety and Handling information.Please refer to the Hexion web site for Shelf Life and recommended Storage information.For ease of handling and optimum shelf life, epoxy dispersions should be stored in tightly sealed containers at temperatures between 50 °F (10°C) and 100 °F (37.8 °C). Do not allow the product to freeze. To prevent skinning or surface drying, do not leave the product uncovered for extended periods of time. If the need arises to store partially filled drums, replace the plastic top-sheet onto the surface of the liquid product.Exposure to these materials should be minimized and avoided, if feasible, through the observance of proper precautions, use of appropriate engineering controls and proper personal protective clothing and equipment, and adherence to proper handling procedures. None of thesematerials should be used, stored, or transported until the handling precautions and recommendations as stated in the Safety Data Sheet (SDS) for these and all other products being used are understood by all persons who will work with them. Questions and requests for information on Hexion Inc. ("Hexion") products should be directed to your Hexion sales representative, or the nearest Hexion sales office. Information and SDSs on non-Hexion products should be obtained from the respective manufacturer.PackagingAvailable in bulk and drum quantities.Contact InformationFo r p ro d u ct p ri ce s, a va i l a b i l i ty, o r o rd e r p l a ce m e n t, vi s i t th e “Co n ta ct Us” s e c o n o f o u r w e b s i te. Fo r l i te ra tu re a n d te ch n i ca l a s s i s ta n ce, vi s i t o u r w e b s i te a t: w w w.H e xi o n.co m/e p o xy。

无内毒素质粒小量提取试剂盒使用说明

无内毒素质粒小量提取试剂盒使用说明
9、 向 吸 附 柱 中 加 入 700ul 漂 洗 液 (使 用 前 请 先 检 查 是 否 已 加 入 无 水 乙 醇 ), 12000rpm 离 心 1min, 弃 废 液 , 将 吸 附 柱 放 入 收 集 管 中 。
10、 向 吸 附 柱 中 加 入 500ul 漂 洗 液 , 12000rpm 离 心 1min, 弃 废 液 , 将吸附柱放入收集管中。
3、向 离 心 管 中 加 入 200ul 溶 液 P2,温 和 地 上 下 翻 转 6-8 次 使 菌 体 充 分 裂 解 。 注 意 : 混 匀 一 定 要 温 和 , 以 免 污 染 细 菌 基 因 组 DNA, 此 时 菌 液 应 变 得 清 亮 粘 稠 , 作 用 时 间 不 要 超 过 5 min, 以 免 质 粒 受 到 破 坏 。
2. 洗 脱 缓 冲 液 体 积 不 应 少 于 50ul, 体 积 过 小 影 响 回 收 效 率 ; 洗 脱 液 的

pH 值 对 洗 脱 效 率 也 有 影 晌 ,若 需 要 用 水 做 洗 脱 液 应 保 证 其 pH 值 在 8.0 左 右 (可 用 NaOH 将 水 的 pH 值 调 至 此 范 围 ), pH 值 低 于 7. 0 会 降 低 洗 脱 效 率。
由于质粒dna本身的性质清除过程可导致部分质粒dna丢失但内毒素却能得到最大限度清所有溶液应用无内毒素的高纯水配制所有器械材料均应不含内毒素玻璃器皿可高温烘烤非挥发性水溶液可高压处理

无内毒素质粒小量提取试剂盒使用说明 货 号 : D1140 规 格 : 50T/100T 保存:常温干燥保存,复检期为一年。 试剂盒内容:

有 机 化 合 物 。 内 毒 素 清 除 剂 可 最 大 限 度 地 除 去 内 毒 素。 从 1-5ml 大 肠 杆 菌 LB 培 养 液 中 ,可 快 速 提 取 5-15μ g 高 纯 度 质 粒 DNA,提 取 率 达 85-90%。使 用 本 试 剂 盒 提 取 的 质 粒 DNA 纯 度 高 ,可 直 接 用 于 细 胞 转 染 等 要 求 较 高 的 实 验 , 以 及 其 他 各 种 常 规 操 作 , 包 括 酶 切 、 PCR、 测 序 、 连 接 和 转 化 等 试 验 。

超氧化物歧化酶质检编号

超氧化物歧化酶质检编号

超氧化物歧化酶质检编号全文共四篇示例,供读者参考第一篇示例:超氧化物歧化酶(superoxide dismutase,SOD)是一种细胞内的重要抗氧化酶,能够将细胞内超氧化物(O2-)转化为氧气(O2)和过氧化氢(H2O2),从而帮助细胞抵抗氧化应激和减少细胞损伤。

SOD在机体的生理过程中起着重要的作用,对维持细胞内氧化还原平衡具有重要意义。

随着现代医学技术的进步,人们对超氧化物歧化酶进行了深入研究,并开发出了对SOD进行质检的方法。

质检编号是对SOD的一种质量标准检测,可以用来评估SOD的纯度和活性水平,从而保证其在医学和科研领域的应用效果。

质检编号主要包括对SOD的纯度、活性和稳定性等方面的检测,以下将从这几个方面对SOD的质检编号进行介绍。

其次是SOD的活性检测。

SOD的活性是其在细胞内发挥生理功能的关键所在,因此活性检测是SOD质检编号中至关重要的一环。

活性检测主要通过化学方法或生物学方法,如抗氧化能力检测、超氧化物歧化反应检测等,来评估SOD样品的活性水平。

只有经过活性检测合格的SOD样品才能保证其在使用过程中具有良好的效果。

SOD的质检编号是对其纯度、活性和稳定性等方面的综合评估,可以帮助人们了解SOD的品质和使用效果。

通过对SOD进行质检编号,可以提高其在医学和科研领域的应用效果,保证其在细胞内的抗氧化作用,从而提高机体的免疫力和抗氧化能力。

希望未来能够通过不断的研究和质检,为人类的健康和生活质量带来更多的改善和帮助。

【2000字】第二篇示例:超氧化物歧化酶(superoxide dismutase,SOD)是一种重要的抗氧化酶,在生物体内发挥着关键的保护作用。

它能够催化超氧自由基的歧化反应,将有害的超氧分子转化为对细胞更为安全的分子,从而保护细胞免受氧化应激的损害。

超氧化物歧化酶不仅在人体内起着重要的作用,也在其他生物体中被广泛存在。

为了检测超氧化物歧化酶的质量,科研工作者们研发出了一种特殊的质检编号系统,以确保产品的质量和可靠性。

碧云天NAC抗氧化剂产品说明书

碧云天NAC抗氧化剂产品说明书

碧云天生物技术/Beyotime Biotechnology 订货热线:400-168-3301或800-8283301 订货e-mail :******************技术咨询:*****************网址:碧云天网站 微信公众号NAC (抗氧化剂) (≥99%, Reagent grade)产品编号 产品名称包装 ST1546-2g NAC (抗氧化剂) (≥99%, Reagent grade) 2g ST1546-10g NAC (抗氧化剂) (≥99%, Reagent grade) 10g ST1546-50gNAC (抗氧化剂) (≥99%, Reagent grade)50g产品简介:CAS Number Chemical FormulaMolecular WeightPurity Grade 616-91-1C 5H 9NO 3S163.19≥99%Reagent grade基本信息(General Information ):Name (Chinese) NAC (抗氧化剂) Name (English) NAC|N-Acetyl-L-cysteine Specifications Reagent grade, ≥99% (TLC), powder Chemical Formula C 5H 9NO 3S Synonym (Chinese) LNAC, NAC, N -乙酰基-L-半胱氨酸 Synonym (English) LNAC; NAC Beilstein Registry No. 1724426 EINECS Number 210-498-3 MDL Number MFCD00004880 UNSPSC Code 12352209 产品描述(Description):Biochem/physiol Actions抗氧化剂和粘液溶解剂。

增加细胞库的自由基捕获剂。

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项目名称:中国石油独山子石化公司中国石油
独山子石化改扩建炼油及新建乙烯工程
装置名称:乙烯装置
资质等级 : 甲级 证书编号:010016-sj 文件号D200501-21010-FU0204-0003
页数3
C0 详细设计
修改
说 明
编制 校核 审核 审定 项目经理日期
裂解炉
(10-F-0101~10-F-0801) 离心铸造管感应弯管
工程规定
目录
1 总则 (2)
2 标准及规范 (2)
3 材料 (2)
4 制造及检验 (2)
1 总则
1.1适用范围
1)本工程规定适用于独山子100万吨/年乙烯工程乙烯装置裂解炉(10-F-0101~10-F-0801)离心铸造管感应弯管的制造、试验、检验及验收要求,并作为离心铸造炉管询价文件的组成部分。

2)当卖方申请代材或与本规定有偏差或背离时,必须经过设计部门的书面认可。

2 标准及规范
除满足本工程规定的要求外,还应满足以下标准规范及工程规定的相关要求。

当下列标准或规范与本规定出现矛盾或不一致时按要求严格者执行。

标准规范版次应为现行最新版。

中国寰球工程公司设计的图纸及相关规范:
裂解炉辐射段炉管工程图 D200501-21010-FU1104-0002
裂解炉离心铸造炉管工程规定 D200501-21010-FU0204-0001
LINDE公司标准:
LS 145-12 Pipe System For Process Furnace Welding And Testing
LS 011-270 Centrifugally Cast Pipes And Pipe Systems of Heat-Resisting Steel
LS 015-270 Inductive Bent Centrifugally Cast Pipes of Heat-Resisting Steel
3 材料
离心浇注管感应弯管的所用直管应符合HQCEC工程规定
D200501-21010-FU0204-0001《裂解炉离心铸造炉管工程规定》及林德标准 LS 011-270 “Centrifugally Cast Pipes and Pipe Systems of Heat-Resisting Steel”的要求。

4. 制造及检验
4.1 热处理
内表面机加工好的管子在弯曲之前进行退火固溶热处理(消除机加工冷作硬化)。

热处理过程参数应有准确记录文档(环境条件、温度、保温时间、升温与冷却速度、冷却介质)。

弯管厂应对热处理过程参数的适用性、准确性负责。

4.2 在正式弯制之前,应根据管子的材料、直径、壁厚按管子的最小弯曲半径制作
样件,以验证弯管工艺。

样件需进行以下步骤检验:
-几何尺寸检验,满足本规定4.5的要求;
-弯管切开;
-切开弯头试件内外表面100%渗透检验;结果应符合ASME Ⅷ,DIV.1,附件
8的要求或GB/T 9443 “1” 级的要求。

-测量弯管内侧与外侧壁厚。

-在壁厚最薄处取微观剖面,在平行管子轴线方向上的微观剖面上不得有裂纹,最大允许其他缺陷显示深度为:50μm,并达到HQCEC 工程规定《离心铸
造炉管工程规定》(D200501-21010-FU0204-0001) 4.1.2、4.1.3、4.1.4的要求-任何弯管工艺不得改变原有材料的金相组织结构。

4.3 弯管应无任何皱折、凹陷、机械伤痕等缺陷。

4.4 所有弯管在弯曲后的最小厚度不得小于图样中规定的最小密实层厚度。

4.5 弯管尺寸公差:
-弯曲半径:±1%
-弯曲角度:±0.5°
-线性尺寸:±2mm
-弯曲部位不圆度偏差: 2mm
-设计图样中所标公差
4.6 制造与检验:
每个产品需进行以下检验:
-检查标记;
-几何尺寸检验;
-坡口与外表面100%渗透检验;坡口渗透检验结果应符合ASME Ⅷ,DIV.1,附件8的要求或JB/T 4730.5中I级的要求。

外表面渗透检验应符合ASME Ⅷ,DIV.1,附件8的要求或GB/T 9443 “1” 级的要求。

-内表面100%内窥镜(至少放大5倍)检验;达到HQCEC 工程规定《离心铸造炉管工程规定》4.1.2、4.1.3的要求。

-检验结果应记录归档。

4.7 除注明外,弯管工艺参照《电站弯管》DL/T 515-2004中的相关规定。

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