Mosapride_citrate_SDS_MedChemExpress
SPACE_peptide_SDS_MedChemExpress
Inhibitors, Agonists, Screening LibrariesSafety Data Sheet Revision Date:May-24-2017Print Date:May-24-20171. PRODUCT AND COMPANY IDENTIFICATION1.1 Product identifierProduct name :SPACE peptideCatalog No. :HY-P0123CAS No. :None1.2 Relevant identified uses of the substance or mixture and uses advised againstIdentified uses :Laboratory chemicals, manufacture of substances.1.3 Details of the supplier of the safety data sheetCompany:MedChemExpress USATel:609-228-6898Fax:609-228-5909E-mail:sales@1.4 Emergency telephone numberEmergency Phone #:609-228-68982. HAZARDS IDENTIFICATION2.1 Classification of the substance or mixtureNot a hazardous substance or mixture.2.2 GHS Label elements, including precautionary statementsNot a hazardous substance or mixture.2.3 Other hazardsNone.3. COMPOSITION/INFORMATION ON INGREDIENTS3.1 SubstancesSynonyms:AC⁻TGSTQHQ⁻CG,Disulfide Bridge 2–10Formula:C40H63N15O17S2Molecular Weight:1090.17CAS No. :None4. FIRST AID MEASURES4.1 Description of first aid measuresEye contactRemove any contact lenses, locate eye-wash station, and flush eyes immediately with large amounts of water. Separate eyelids with fingers to ensure adequate flushing. Promptly call a physician.Skin contactRinse skin thoroughly with large amounts of water. Remove contaminated clothing and shoes and call a physician.InhalationImmediately relocate self or casualty to fresh air. If breathing is difficult, give cardiopulmonary resuscitation (CPR). Avoid mouth-to-mouth resuscitation.IngestionWash out mouth with water; Do NOT induce vomiting; call a physician.4.2 Most important symptoms and effects, both acute and delayedThe most important known symptoms and effects are described in the labelling (see section 2.2).4.3 Indication of any immediate medical attention and special treatment neededTreat symptomatically.5. FIRE FIGHTING MEASURES5.1 Extinguishing mediaSuitable extinguishing mediaUse water spray, dry chemical, foam, and carbon dioxide fire extinguisher.5.2 Special hazards arising from the substance or mixtureDuring combustion, may emit irritant fumes.5.3 Advice for firefightersWear self-contained breathing apparatus and protective clothing.6. ACCIDENTAL RELEASE MEASURES6.1 Personal precautions, protective equipment and emergency proceduresUse full personal protective equipment. Avoid breathing vapors, mist, dust or gas. Ensure adequate ventilation. Evacuate personnel to safe areas.Refer to protective measures listed in sections 8.6.2 Environmental precautionsTry to prevent further leakage or spillage. Keep the product away from drains or water courses.6.3 Methods and materials for containment and cleaning upAbsorb solutions with finely-powdered liquid-binding material (diatomite, universal binders); Decontaminate surfaces and equipment by scrubbing with alcohol; Dispose of contaminated material according to Section 13.7. HANDLING AND STORAGE7.1 Precautions for safe handlingAvoid inhalation, contact with eyes and skin. Avoid dust and aerosol formation. Use only in areas with appropriate exhaust ventilation.7.2 Conditions for safe storage, including any incompatibilitiesKeep container tightly sealed in cool, well-ventilated area. Keep away from direct sunlight and sources of ignition.Recommended storage temperature:Powder-20°C 3 years4°C 2 yearsIn solvent-80°C 6 months-20°C 1 monthShipping at room temperature if less than 2 weeks.7.3 Specific end use(s)No data available.8. EXPOSURE CONTROLS/PERSONAL PROTECTION8.1 Control parametersComponents with workplace control parametersThis product contains no substances with occupational exposure limit values.8.2 Exposure controlsEngineering controlsEnsure adequate ventilation. Provide accessible safety shower and eye wash station.Personal protective equipmentEye protection Safety goggles with side-shields.Hand protection Protective gloves.Skin and body protection Impervious clothing.Respiratory protection Suitable respirator.Environmental exposure controls Keep the product away from drains, water courses or the soil. Cleanspillages in a safe way as soon as possible.9. PHYSICAL AND CHEMICAL PROPERTIES9.1 Information on basic physical and chemical propertiesAppearance White to off-white (Solid)Odor No data availableOdor threshold No data availablepH No data availableMelting/freezing point No data availableBoiling point/range No data availableFlash point No data availableEvaporation rate No data availableFlammability (solid, gas)No data availableUpper/lower flammability or explosive limits No data availableVapor pressure No data availableVapor density No data availableRelative density No data availableWater Solubility No data availablePartition coefficient No data availableAuto-ignition temperature No data availableDecomposition temperature No data availableViscosity No data availableExplosive properties No data availableOxidizing properties No data available9.2 Other safety informationNo data available.10. STABILITY AND REACTIVITY10.1 ReactivityNo data available.10.2 Chemical stabilityStable under recommended storage conditions.10.3 Possibility of hazardous reactionsNo data available.10.4 Conditions to avoidNo data available.10.5 Incompatible materialsStrong acids/alkalis, strong oxidising/reducing agents.10.6 Hazardous decomposition productsUnder fire conditions, may decompose and emit toxic fumes.Other decomposition products - no data available.11.TOXICOLOGICAL INFORMATION11.1 Information on toxicological effectsAcute toxicityClassified based on available data. For more details, see section 2Skin corrosion/irritationClassified based on available data. For more details, see section 2Serious eye damage/irritationClassified based on available data. For more details, see section 2Respiratory or skin sensitizationClassified based on available data. For more details, see section 2Germ cell mutagenicityClassified based on available data. For more details, see section 2CarcinogenicityIARC: No component of this product present at a level equal to or greater than 0.1% is identified as probable, possible or confirmed human carcinogen by IARC.ACGIH: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by ACGIH.NTP: No component of this product present at a level equal to or greater than 0.1% is identified as a anticipated or confirmed carcinogen by NTP.OSHA: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by OSHA.Reproductive toxicityClassified based on available data. For more details, see section 2Specific target organ toxicity - single exposureClassified based on available data. For more details, see section 2Specific target organ toxicity - repeated exposureClassified based on available data. For more details, see section 2Aspiration hazardClassified based on available data. For more details, see section 212. ECOLOGICAL INFORMATION12.1 ToxicityNo data available.12.2 Persistence and degradabilityNo data available.12.3 Bioaccumlative potentialNo data available.12.4 Mobility in soilNo data available.12.5 Results of PBT and vPvB assessmentPBT/vPvB assessment unavailable as chemical safety assessment not required or not conducted.12.6 Other adverse effectsNo data available.13. DISPOSAL CONSIDERATIONS13.1 Waste treatment methodsProductDispose substance in accordance with prevailing country, federal, state and local regulations.Contaminated packagingConduct recycling or disposal in accordance with prevailing country, federal, state and local regulations.14. TRANSPORT INFORMATIONDOT (US)This substance is considered to be non-hazardous for transport.IMDGThis substance is considered to be non-hazardous for transport.IATAThis substance is considered to be non-hazardous for transport.15. REGULATORY INFORMATIONSARA 302 Components:No chemicals in this material are subject to the reporting requirements of SARA Title III, Section 302.SARA 313 Components:This material does not contain any chemical components with known CAS numbers that exceed the threshold (De Minimis) reporting levels established by SARA Title III, Section 313.SARA 311/312 Hazards:No SARA Hazards.Massachusetts Right To Know Components:No components are subject to the Massachusetts Right to Know Act.Pennsylvania Right To Know Components:No components are subject to the Pennsylvania Right to Know Act.New Jersey Right To Know Components:No components are subject to the New Jersey Right to Know Act.California Prop. 65 Components:This product does not contain any chemicals known to State of California to cause cancer, birth defects, or anyother reproductive harm.16. OTHER INFORMATIONCopyright 2017 MedChemExpress. The above information is correct to the best of our present knowledge but does not purport to be all inclusive and should be used only as a guide. The product is for research use only and for experienced personnel. It must only be handled by suitably qualified experienced scientists in appropriately equipped and authorized facilities. The burden of safe use of this material rests entirely with the user. MedChemExpress disclaims all liability for any damage resulting from handling or from contact with this product.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。
蛋白裂解液
之蔡仲巾千创作蛋白质的定位与蛋白质裂解液的选择:全细胞:NP-40或RIPA细胞质(可溶):Tris-HCl细胞质(细胞骨架):Tris-Triton细胞膜:NP-40或RIPA细胞核:RIPA线粒体:RIPARIPA裂解液配方1ml RIPA buffer contains 35μl proteinase inhibitorand 10μl phosphatase inhibitor加水到 100mlAprotinin 10μg/μl ;用10mM HEPES-KOH(PH8.0)溶解,备用。
●Nonidet P-40简称NP-40,乙基苯基聚乙二醇,经常使用非离子性去垢剂。
●Na-deoxycholale,脱氧胆酸钠,离子型去垢剂。
离子型去垢剂主要作用有:裂解细胞;溶解蛋白,尤其是可以溶解一些难溶于水的蛋白,如膜蛋白等;很适合做WB,但在Co-IP中使用,需要谨慎。
●亮抑霉肽(Leupeptin):抑制丝氨酸蛋白酶包含胰蛋白酶、纤溶酶、猪胰激肽原酶)和半胱氨酸蛋白酶(包含木瓜蛋白酶和组织蛋白酶B)●抑肽素A(Pepstatin A)抑制各种天门冬氨酸蛋白酶,例如组织蛋白酶D、肾素、胃蛋白酶、细菌天门冬氨酸蛋白酶和HIV蛋白酶;●胰凝乳蛋白酶抑制剂(Chymostatin)抑制具有糜蛋白酶类特异性丝氨酸蛋白酶(包含糜蛋白酶、胃促胰酶、组织蛋白酶 G)和大多数丝氨酸蛋白酶(包含组织蛋白酶B,H,L)●抗木瓜蛋白酶(Antipain)抑制木瓜蛋白酶、胰蛋白酶和纤溶酶。
●氟化钠抑制酸性磷酸酶●正钒酸钠:抑制碱性磷酸酶和酪氨酸磷酸酶(tyrosine phosphatase)●焦磷酸钠(sodium pyrophosphate):抑制丝氨酸-苏氨酸磷酸酶(serine-threonine phosphatase)。
在与蛋白相关的检测中,首先最关键的一步即是蛋白质的提取。
蛋白质的提取过程中,我们要经常加和蛋白酶抑制剂以防止蛋白质的降解。
迷迭香酸对D-半乳糖致衰老小鼠抗氧化作用的研究
迷迭香酸对D-半乳糖致衰老小鼠抗氧化作用的研究王虹;刘敏;顾建祥;顾建勇【期刊名称】《中国老年学杂志》【年(卷),期】2009(29)5【摘要】目的观察迷迭香酸对D-半乳糖诱导的衰老小鼠的抗氧化作用.方法昆明小鼠连续6 w颈背部皮下注射D-半乳糖1mg·g-1·d-1复制亚急性衰老小鼠模型.造模的过程中,3个给药组(低、中、高剂量) 分别以100、200和400 mg·kg-1·d-1的迷迭香酸分析纯灌胃;同时设立3个含有等效剂量迷迭香酸的迷迭香提取物给药组(1 000、2 000和4 000 mg·kg-1·d-1)对比灌胃.实验6 w后处死动物,测定血清和脑中超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量、谷胱甘肽过氧化物酶(GSH-Px)活性、甘油三酯(TG)水平;以及进行耐缺氧实验. 结果高剂量的迷迭香酸能明显提高衰老小鼠血清和脑中的SOD活力(P<0.01)、GSH-Px活性(P<0.05),降低MDA含量(P<0.05)和TG水平(P<0.05),且能明显延长小鼠常压耐缺氧时间(P<0.05).结论迷迭香酸通过提高抗氧化酶活性,清除自由基,减少过氧化脂质生成而抗衰老.【总页数】3页(P549-551)【作者】王虹;刘敏;顾建祥;顾建勇【作者单位】泰山医学院化学与化学工程学院,山东,泰安,271016;泰安市中心医院妇科,山东,泰安,271016;泰安市中医医院骨科,山东,泰安,271016;泰安大凡神农制药公司,山东,泰安,271016【正文语种】中文【中图分类】R285.5【相关文献】1.松蘑提取液对D-半乳糖致衰老小鼠抗氧化作用的研究 [J], 栾海云;王桂华;许勇;杨茗2.大枣对D-半乳糖致衰老小鼠抗氧化作用的研究 [J], 羊小胜;胡春威3.类叶升麻苷对D-半乳糖致衰老小鼠抗氧化作用的研究 [J], 高莉;林娟;张富春;霍仕霞;彭晓明;闫明4.米酒对D-半乳糖致衰老小鼠抗氧化作用的研究 [J], 陈欲云;刘春丽;王涛;边名鸿;胡婷婷;谢凌冬5.维生素A对D-半乳糖致衰老小鼠抗氧化作用的研究 [J], 王俐;冯雪萍;吕柔;岑花鲜;林朝旗;韦金花;谢尤祥;杨敏;郭子鹏;韦芃因版权原因,仅展示原文概要,查看原文内容请购买。
Cisplatin_SDS_MedChemExpress
Inhibitors, Agonists, Screening LibrariesSafety Data Sheet Revision Date:Jul.-12-2017Print Date:Jul.-12-20171. PRODUCT AND COMPANY IDENTIFICATION1.1 Product identifierProduct name :CisplatinCatalog No. :HY-17394CAS No. :15663-27-11.2 Relevant identified uses of the substance or mixture and uses advised againstIdentified uses :Laboratory chemicals, manufacture of substances.1.3 Details of the supplier of the safety data sheetCompany:MedChemExpress USATel:609-228-6898Fax:609-228-5909E-mail:sales@1.4 Emergency telephone numberEmergency Phone #:609-228-68982. HAZARDS IDENTIFICATION2.1 Classification of the substance or mixtureGHS Classification in accordance with 29 CFR 1910 (OSHA HCS)Acute toxicity, Oral (Category 2), H300Serious eye damage (Category 1), H318Carcinogenicity (Category 1B), H3502.2 GHS Label elements, including precautionary statementsPictogramSignal word DangerHazard statement(s)H300 Fatal if swallowed.H318 Causes serious eye damage.H350 May cause cancer.Precautionary statement(s)P201 Obtain special instructions before use.P202 Do not handle until all safety precautions have been read and understood.P264 Wash skin thoroughly after handling.P270 Do not eat, drink or smoke when using this product.P280 Wear protective gloves/ eye protection/ face protection.P301 + P310 IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician.P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contactlenses, if present and easy to do. Continue rinsing.P310 Immediately call a POISON CENTER or doctor/ physician.H300 Fatal if swallowed.H318 Causes serious eye damage.H350 May cause cancer. P321 Specific treatment (see supplemental first aid instructions on this label).P330 Rinse mouth.P405 Store locked up.P501 Dispose of contents/ container to an approved waste disposal plant.2.3 Other hazardsNone.3. COMPOSITION/INFORMATION ON INGREDIENTS3.1 SubstancesSynonyms:CDDP; cis–DiaminodichloroplatinumFormula:Cl2H6N2PtMolecular Weight:300.05CAS No. :15663-27-14. FIRST AID MEASURES4.1 Description of first aid measuresEye contactRemove any contact lenses, locate eye-wash station, and flush eyes immediately with large amounts of water. Separate eyelids with fingers to ensure adequate flushing. Promptly call a physician.Skin contactRinse skin thoroughly with large amounts of water. Remove contaminated clothing and shoes and call a physician.InhalationImmediately relocate self or casualty to fresh air. If breathing is difficult, give cardiopulmonary resuscitation (CPR). Avoid mouth-to-mouth resuscitation.IngestionWash out mouth with water; Do NOT induce vomiting; call a physician.4.2 Most important symptoms and effects, both acute and delayedThe most important known symptoms and effects are described in the labelling (see section 2.2).4.3 Indication of any immediate medical attention and special treatment neededTreat symptomatically.5. FIRE FIGHTING MEASURES5.1 Extinguishing mediaSuitable extinguishing mediaUse water spray, dry chemical, foam, and carbon dioxide fire extinguisher.5.2 Special hazards arising from the substance or mixtureDuring combustion, may emit irritant fumes.5.3 Advice for firefightersWear self-contained breathing apparatus and protective clothing.6. ACCIDENTAL RELEASE MEASURES6.1 Personal precautions, protective equipment and emergency proceduresUse full personal protective equipment. Avoid breathing vapors, mist, dust or gas. Ensure adequate ventilation. Evacuate personnel to safe areas.Refer to protective measures listed in sections 8.6.2 Environmental precautionsTry to prevent further leakage or spillage. Keep the product away from drains or water courses.6.3 Methods and materials for containment and cleaning upAbsorb solutions with finely-powdered liquid-binding material (diatomite, universal binders); Decontaminate surfaces and equipment by scrubbing with alcohol; Dispose of contaminated material according to Section 13.7. HANDLING AND STORAGE7.1 Precautions for safe handlingAvoid inhalation, contact with eyes and skin. Avoid dust and aerosol formation. Use only in areas with appropriate exhaust ventilation.7.2 Conditions for safe storage, including any incompatibilitiesKeep container tightly sealed in cool, well-ventilated area. Keep away from direct sunlight and sources of ignition.Recommended storage temperature:Powder-20°C 3 years4°C 2 yearsIn solvent-80°C 6 months-20°C 1 monthShipping at room temperature if less than 2 weeks.7.3 Specific end use(s)No data available.8. EXPOSURE CONTROLS/PERSONAL PROTECTION8.1 Control parametersComponents with workplace control parametersThis product contains no substances with occupational exposure limit values.8.2 Exposure controlsEngineering controlsEnsure adequate ventilation. Provide accessible safety shower and eye wash station.Personal protective equipmentEye protection Safety goggles with side-shields.Hand protection Protective gloves.Skin and body protection Impervious clothing.Respiratory protection Suitable respirator.Environmental exposure controls Keep the product away from drains, water courses or the soil. Cleanspillages in a safe way as soon as possible.9. PHYSICAL AND CHEMICAL PROPERTIES9.1 Information on basic physical and chemical propertiesAppearance Light yellow to yellow (Solid)Odor No data availableOdor threshold No data availablepH No data availableMelting/freezing point No data availableBoiling point/range No data available Flash point No data available Evaporation rate No data available Flammability (solid, gas)No data available Upper/lower flammability or explosive limits No data available Vapor pressure No data available Vapor density No data available Relative density No data available Water Solubility No data available Partition coefficient No data available Auto-ignition temperature No data available Decomposition temperature No data available Viscosity No data available Explosive properties No data available Oxidizing properties No data available 9.2 Other safety informationNo data available.10. STABILITY AND REACTIVITY10.1 ReactivityNo data available.10.2 Chemical stabilityStable under recommended storage conditions.10.3 Possibility of hazardous reactionsNo data available.10.4 Conditions to avoidNo data available.10.5 Incompatible materialsStrong acids/alkalis, strong oxidising/reducing agents.10.6 Hazardous decomposition productsUnder fire conditions, may decompose and emit toxic fumes.Other decomposition products - no data available.11.TOXICOLOGICAL INFORMATION11.1 Information on toxicological effectsAcute toxicityClassified based on available data. For more details, see section 2Skin corrosion/irritationClassified based on available data. For more details, see section 2Serious eye damage/irritationClassified based on available data. For more details, see section 2Respiratory or skin sensitizationClassified based on available data. For more details, see section 2Germ cell mutagenicityClassified based on available data. For more details, see section 2CarcinogenicityIARC: No component of this product present at a level equal to or greater than 0.1% is identified as probable, possible or confirmed human carcinogen by IARC.ACGIH: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by ACGIH.NTP: No component of this product present at a level equal to or greater than 0.1% is identified as a anticipated or confirmed carcinogen by NTP.OSHA: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by OSHA.Reproductive toxicityClassified based on available data. For more details, see section 2Specific target organ toxicity - single exposureClassified based on available data. For more details, see section 2Specific target organ toxicity - repeated exposureClassified based on available data. For more details, see section 2Aspiration hazardClassified based on available data. For more details, see section 2Additional informationRTECS No.: TP2450000May cause liver irregularities.This information is based on our current knowledge. However the chemical, physical, and toxicological properties have not been completely investigated.12. ECOLOGICAL INFORMATION12.1 ToxicityNo data available.12.2 Persistence and degradabilityNo data available.12.3 Bioaccumlative potentialNo data available.12.4 Mobility in soilNo data available.12.5 Results of PBT and vPvB assessmentPBT/vPvB assessment unavailable as chemical safety assessment not required or not conducted.12.6 Other adverse effectsNo data available.13. DISPOSAL CONSIDERATIONS13.1 Waste treatment methodsProductDispose substance in accordance with prevailing country, federal, state and local regulations.Contaminated packagingConduct recycling or disposal in accordance with prevailing country, federal, state and local regulations.14. TRANSPORT INFORMATIONDOT (US)This substance is considered to be non-hazardous for transport.IMDGThis substance is considered to be non-hazardous for transport.IATAThis substance is considered to be non-hazardous for transport.15. REGULATORY INFORMATIONAcute Health Hazard, Chronic Health Hazard.16. OTHER INFORMATIONCopyright 2017 MedChemExpress. The above information is correct to the best of our present knowledge but does not purport to be all inclusive and should be used only as a guide. The product is for research use only and for experienced personnel. It must only be handled by suitably qualified experienced scientists in appropriately equipped and authorized facilities. The burden of safe use of this material rests entirely with the user. MedChemExpress disclaims all liability for any damage resulting from handling or from contact with this product.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。
HPLC法测定枸橼酸莫沙必利分散片的有关物质
山东化工SHANDONG CHEMICAL INDUSTRY・82・2020年第49卷i分析与测试]HPLC法测定枸椽酸莫沙必利分散片的有关物质孙亚敏,栾会妮,徐勤娟,李锡勇(威海海洋职业学院,山东威海264300;)摘要:目的:建立枸椽酸莫沙必利分散片有关物质的检查方法%方法:采用We-h SX-C k色谱柱(4.6xl50mm,5!m);以0.05mol/P的枸椽酸溶液(用2mol/C的氢氧化钠溶液调节pH值至4.0)为流动相A,乙g为流动相B,进行梯度洗脱;检测波长为274nm;柱温为405%结果:莫沙必利主峰与相邻杂质分离度良好,莫沙必利、杂质A、杂质B和杂质C的线性范围分别为0.25~6.00!g/mL-1(c09999)、。
中5〜6K0/!g・mL-(o0.9999)、0中5〜6K5!g/mL&(r-0.9998)&0.25~6O1/!g・mL-( 1.0000),莫沙必利、杂质A、杂质B和杂质C的检测限分别为0.7ng&0.3ng^tJ)ng和0.7ng,定量限分别为2.5cg、lK cg、5O ng和2.5ng,杂质A、B、C低、中、高3种浓度的平均回收率分别为98.53%&99.01%和101.28%(n-9)%结论:经方法学验证,所建立的方法专属性、线性、准确度、重复性好,本法适用于枸椽酸莫沙必利分散片有关物质检查%关键词:枸椽酸莫沙必利分散片;有关物质;方法学验证;高效液相色谱法中图分类号:0657.7;917文献标识码:A文章编号:1008-021X(2020)19-0082-05HPLC determination of relates substances in mosaprine citratr dispersible tabletiSun Yami n,Luan Humi,Xu Qinjuan,Li Xiyong(Weihai Marine vocational col—ae,Weihai264300,China)Abstract:Objective:To establish an HPLC method for the determination of the mosapride citrato dispersib—tablets and its related :ub:iance:.Meihod::Thedeieemcnaicon wa:ca e ced ouion aWe—ch SX-C18(4.6h150mm,5!m)co—umn,wcih0.05mo—・L-1 citric acid solution(by2mo—C sodium hydroxide adjust to pH4.0)as mobile phase A,with acetonitrile as mobile phase B in a gradAnt elution.The/ow rate was1.0mL・min1,the UV detection wavelength was set at274nm and the column mmperamN was405.Resu—is:Themacn subsianceand cisadeacenicmpuecicescou—d besepaeaied adequaie—ywcih awe—-eequceed eeso—uicon.The—near ranges of mosapride,impurity A,impuriA B and impurity C were0.25〜6.00!g/mL1(w0.9999),0中5〜6.00!g/ mL_1(r=0.9999),0.25〜6O5!g/mL—(w0.9998)and0.25~6O1!g/mL_1(r=1.0000),respectively.The detection limits of mo:apacde,cmpuactyA,cmpuactyBand cmpuactyCweae0.7ng,0.3ng,1.5ngand0.7ng,ae:pectcee—y.Thequantctatcon—cmctoe mo:apecde,cmpueciyA,cmpueciyBand cmpueciyCweee2.5ng, 1.0ng, 5.0ngand2.5ng,ee:pecicee—y.Theaeeeageeecoeee eaie:weee98.53%、99.01%and101.28%ee:pecicee—yai—ow,medcum and hcgh concen iea icon:(n=9).Conc—u:con:The me ihod cea—cdaied ihaiciha:good:pecceccciy,—cneaeciy,accueacy,and eepeaiabc—ciy.Thcmeihod can beu:ed eoeiheee—aied:ub:iance conieo—oemo:apecdeccieaiedcpeecb—eiab—ei.Key words:mosapride citrato dispersib—tablets;related substances;method validation;HPLC枸椽酸莫沙必利(Mosapride Citrate)为大日本制药株式会社开发的胃动力药,是一强效选择性5-径色胺(5-HT4)受体激动剂,通过兴奋肌间神经丛的5-HT4受体,刺激乙酰胆碱释放,从而增强胃肠运动,但不影响胃酸分泌,是一安全有效的胃动力药临床用于慢性胃炎、功能性消化不良、反流性食管炎及手术伴随的一系列胃肠道症状的缓解*3+。
蛋白裂解液
之邯郸勺丸创作蛋白质的定位与蛋白质裂解液的选择:全细胞:NP-40或RIPA细胞质(可溶):Tris-HCl细胞质(细胞骨架):Tris-Triton细胞膜:NP-40或RIPA细胞核:RIPA线粒体:RIPARIPA裂解液配方1ml RIPA buffer contains 35μl proteinase inhibitorand 10μl phosphatase inhibitor加水到 100mlAprotinin 10μg/μl ;用10mM HEPES-KOH(PH8.0)溶解,备用.●Nonidet P-40简称NP-40,乙基苯基聚乙二醇,经常使用非离子性去垢剂.●Na-deoxycholale,脱氧胆酸钠,离子型去垢剂.离子型去垢剂主要作用有:裂解细胞;溶解蛋白,尤其是可以溶解一些难溶于水的蛋白,如膜蛋白等;很适合做WB,但在Co-IP中使用,需要谨慎.●亮抑霉肽(Leupeptin):抑制丝氨酸蛋白酶包含胰蛋白酶、纤溶酶、猪胰激肽原酶)和半胱氨酸蛋白酶(包含木瓜蛋白酶和组织蛋白酶B)●抑肽素A(Pepstatin A)抑制各类天门冬氨酸蛋白酶,例如组织蛋白酶D、肾素、胃蛋白酶、细菌天门冬氨酸蛋白酶和HIV蛋白酶;●胰凝乳蛋白酶抑制剂(Chymostatin)抑制具有糜蛋白酶类特异性丝氨酸蛋白酶(包含糜蛋白酶、胃促胰酶、组织蛋白酶 G)和大多数丝氨酸蛋白酶(包含组织蛋白酶B,H,L)●抗木瓜蛋白酶(Antipain)抑制木瓜蛋白酶、胰蛋白酶和纤溶酶.●氟化钠抑制酸性磷酸酶●正钒酸钠:抑制碱性磷酸酶和酪氨酸磷酸酶(tyrosine phosphatase)●焦磷酸钠(sodium pyrophosphate):抑制丝氨酸-苏氨酸磷酸酶(serine-threonine phosphatase) .在与蛋白相关的检测中,首先最关头的一步即是蛋白质的提取.蛋白质的提取过程中,我们要经常加和蛋白酶抑制剂以避免蛋白质的降解.另外在磷酸化蛋白的研究过程中,磷酸酶抑制剂也是必不成少的,本文总结了经常使用的蛋白酶抑制剂PMSF,Leupeptin 亮肽素,Aprotinin抑肽酶,Pepstatin胃蛋白酶抑制剂,EDTA-Na2等以及磷酸酶抑制剂NaF氟化钠,Na3VO4 原矾酸钠 ,BETA-glycerophosphate 甘油磷酸钠,Na2P2O4 焦磷酸钠等.对这些蛋白酶抑制剂的溶解配制,贮存液与任务液浓度,保管都做了详细的说明.蛋白酶抑制剂PMSF:特性:丝氨酸蛋白酶抑制剂,如胰凝乳蛋白酶,胰蛋白酶和凝血酶,也抑制半胱氨酸蛋白酶如木瓜蛋白酶(可逆的地面处理).溶解性:溶于异丙醇,乙醇,甲醇和丙二醇果>10mg/ml.在水溶液中不稳定.在100%异丙醇, +25℃时稳定至少9个月份子量:174.2使用:贮存浓度:200mM,任务浓度:1mMLeupeptin 亮肽素特性:抑制丝氨酸和半胱氨酸蛋白酶如胰蛋白酶,木瓜蛋白酶,纤溶酶,和组织蛋白酶B溶解性:高度溶于水(1mg/ml).4℃一周稳定,分红小份冷冻在-20℃至少6个月份子量:C20H38N6O4 x 1/2 H2SO4:475.6C20H38N6O4 x 1/2 H2SO4 x H2O:493.6使用:贮存浓度:1mg/ml,任务浓度0.5 ug/ml (1mM)Aprotinin抑肽酶特性:丝氨酸蛋白酶抑制剂,抑制纤维蛋白溶酶, 激肽释放酶,胰蛋白酶,糜蛋白酶的高活性.不抑制凝血酶或因子X.溶解性:易溶于水(10mg/ml)或缓冲液(例如,tris,0.1M,pH8.0).pH 约7-8的溶液在4℃可保管1周,分装保管在-20℃可至少保管6个月.避免频频冻融, pH>12.8的碱性环境可使其灭活.份子量:6,512使用:贮存浓度:1mg/ml, 任务浓度:0.06–2.0 ug/ml(0.01–0.3 uM)Pepstatin胃蛋白酶抑制剂特性:抑制天冬氨酸(酸)蛋白酶如胃蛋白酶,肾素,组织蛋白酶D,凝乳酶, 许多微生物酸性蛋白酶溶解性:溶于甲醇约1mg/ml;可溶于乙醇,过夜溶解可达到1 mg/ml;在6当量乙酸中溶解度为300ug/ml.4℃稳定一周,分装储存于-20℃时可保管1个月份子量: 685.9使用:贮存浓度:1mg/ml,使用浓度:0.7 μg/ml(1μM)EDTA-Na2特性:金属蛋白酶抑制剂溶解性:溶于水至0.5M,在pH8-9的条件下,4℃稳定至少6个月份子量: 372.24使用:任务浓度:0.2–0.5 mg/ml(0.5–1.3 mM),不需现用现配,在溶液pH值调至8-9时再加入.磷酸酶抑制剂NaF氟化钠溶解性:溶于水份子量: 41.99使用:贮存液:5M 任务浓度:10-20mMNa3VO4 原矾酸钠份子量:183.91溶解性:溶于水,我们采办过来的是原矾酸钠.原矾酸钠需要经过处理以后才干成为激活的矾酸钠,激活的矾酸钠才具有抑制去磷酸化的作用.原矾酸钠酿成激活的矾酸钠的过程是:100mM原矾酸钠激活储存液配制(1).取0.183克的原矾酸钠溶解于10ml的双蒸水中,加酸调节PH至10(颜色变黄)(2).煮至无色(3).室温冷却(4).重调PH至10(5)重复(1)(2)(3)(4)直至溶液坚持无色,并且PH稳定于10,分装100ul/管,每10ml裂解液加一管.(终浓度为1mM),-20度保管.使用:贮存液:100mM 任务浓度:1mMBETA-glycerophosphate 甘油磷酸钠溶解性:溶于水份子量:306.11使用:贮存液:100mM 任务浓度:25mMNa2P2O4 焦磷酸钠溶解性:溶于水份子量:265.9贮存液:100mM, 任务浓度:1-2 mMSDS ,NP-40 ,TritonX-100这三种去垢剂的作用是不合的,或者说作用力量强弱不合.SDS属于离子型去垢剂,最厉害,基本可以把细胞完全破掉,DNA会释放出来,裂解液变得很粘稠.NP-40是很温和的去垢剂,1%浓度的基本可以破坏掉胞膜,而对核膜破坏的作用弱,结合特定的buffer可以获得胞浆蛋白.TritonX-100的能力介于NP40和SDS之间,偏向于NP40,也是经常使用的细胞裂解液成分之一,在呵护蛋白活性方面有一定作用(SDS 基本会使蛋白变性失活).Brij Buffer I。
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A145N-乙氧碳基氨甲基-L-异亮氨酸A2780人卵巢癌细胞细胞系A549人肺腺癌细胞系aberrant异常abortion流产acceleration加速access访问accessible可访问的acetylaminofluorene乙酰氨基芴(致癌物)aclacinon山之内的商品名adriblastine阿霉素affect影响affection疾患、病、影响alafloxacin阿拉沙星albacort地塞米松amenorrhea闭经amerantrone蒽二酮aminoazotoluene氨基偶氮甲笨(肝脏致癌剂)amniocentesis羊膜穿刺amnion羊膜amnioscope羊膜镜amnioscopy羊膜镜检查amniotomy人工破膜amoxicillin阿莫西林;羟氨苄青霉素amphiregulin双调素,为表皮生长因子家族的成员之一ampulla壶腹部anastomosis吻合术anastrozole阿纳托唑,选择性非甾体类芳香化酶抑制剂。
androblastoma男性母细胞瘤androgen雄激素Anesthetist麻醉医师aneuploid非整倍体angiostatin血管抑制素anitratum硝酸盐阴性(不动杆菌)anovulation不排卵anteflexion前屈antibody抗体antigen抗原antineoplaston抗肿瘤治疗antiretroviral抗逆转录病毒的antisignaling抗信号药物appendix阑尾approximation对合arabitin阿糖腺苷,腺嘌呤阿糖甙Aredia阿可达,帕米膦酸钠(pamidronate)的商品名,Novartis公司出品arimedex芳香化酶抑制剂arimidex新的芳香化酶抑制剂arteriography动脉造影atelectasis肺不张atopic变态反应性autocrineloops自泌环autoinvasive自身侵袭性autosome常染色体AY62013环氧甘醚,乙环氧啶azimexone羧胺氰丙啶(BRMs)B16黑色素瘤细胞系(小鼠)BAK椎体间融合器Ball公司名称;波尔公司;金属制品;网址baseline基线baumanii鲍曼不动杆菌BCDFB细胞分化因子(BRMs)bednet臭虫behavior行为Bel7402人肝癌细胞系Biaoroubixing表阿霉素bioassay生物测定biopsy活检boolean布尔bromoacetoxylphenylhexan溴乙酰己烷雌酚,内消旋3bromodeoxyuridine溴脱氧尿嘧啶,在DNA合成过程中渗入细胞,用于作为进入或经历DNA合成期(S期)的标记,代表DNA合成水平bronchicanis支气管败血症(Bordetella bronchiseptica支气管败血症博代氏菌)BSLi西非单叶豆凝集素,对B型血球专一BSN次硝酸铋(诱导正常组织产生金属硫蛋白,降低化疗药物毒性);bowel sounds normal 肠鸣音正常BTIC氯乙基三氮烯咪唑酰胺BU2231A泰来霉素(博莱霉素衍生物,是一种霉菌产生的糖肽化合物)C12U核酸抗癌剂干扰素诱导剂cabergoline卡麦角林(镇痛药)cackexia恶液质CAI羧基胺基咪唑,是一种细胞信号传导抑制剂,能够阻滞配体受体依赖性Ca2+的内流,改变包括内皮细胞在内的多种细胞的生物行为,如运动、侵袭、酶解和构建血管等,在体内外抑制血管形成calciifolinas亚叶酸calcoacelicus醋酸钙(不动杆菌)Calprotectin钙卫蛋白:钙卫蛋白是近年发现的与钙结合的不均一的复合蛋白质,由2重链及1轻链构成,主要来源于嗜中性粒细胞和单核细胞,分布于粒单细胞、上皮细胞、角质细胞内及各种组织和体液中,具有抗微生物、抗增殖等多种生物学功能。
迷迭香酸对过氧化氢处理下的皮肤黑色素瘤的抗氧化作用(原文翻译)
迷迭香酸(罗丹酚酸)对H2O2处理过的皮肤黑色素瘤细胞的抗氧化作用Sun Mi Yoo1 and Jeong Ran Kang2*1.韩国光州500-741号东冈大学美容系2.韩国首尔143-701号建国大学生物工程系2009.2.6收到 2009.4.17接收本学科旨在检测迷迭香酸对人工孵育的皮肤黑色素瘤细胞在ROS下的抗氧化作用。
通过XTT比色法,以细胞毒性和抗氧化作用来分析细胞粘附活性,DPPH自由基清除活性以及H2O2处理1-10h和未经处理的两种情况下乳酸脱氢酶的活性。
用20-110 μM 的H2O2处理皮肤黑色素瘤细胞5-7h后,细胞活性的降低呈剂量和时间依赖性。
通过XTT比色法测得H2O2的半抑制浓度(IC50 )为90μM。
同时H2O2增强了LDH细胞的剂量依赖性。
用50-90μM的H2O2处理8h后测得LDH50为60 μM H2O2。
迷迭香酸能增强细胞活性和DPPH自由基清除活性,降低乳酸盐脱氢酶的活性。
细胞的H2O2处理证实了对人工孵育的皮肤黑色素瘤细胞的强抗氧化作用。
通过H2O2的处理,迷迭香酸能在细胞内能增强细胞活性和DPPH 自由基清除活性,降低乳酸盐脱氢酶的活性。
这被认为是迷迭香酸对ROS(ROS)如H2O2的抗氧化作用。
Key words:DPPH-radical scavenging, LDH, rosmarinic acid, XTT assay关键字:DPPH自由基清除活性,乳酸脱氢酶,迷迭香酸,XTT比色法据研究发现,ROS通过氧化应激对细胞的损伤和一些脑部疾病比如帕金森症或心脏疾病例如心肌梗塞之间有很大的关联[Difazio et al., 1992; Delanty and Dichter, 1998].尤其是研究人员认为ROS是皮肤老化的一个主要的因素后,一直试图从ROS方面研究衰老。
[Yokozawa et al., 1998].据研究表明,ROS的氧化应激会通过萎缩细胞引起各种疾病,例如超氧自由基、H2O2(H2O2)或羟基自由基的巯基蛋白反应中断酶的活性,破坏脱氧RMA(DNA)或RMA(RNA),诱导细胞膜脂质过氧化。
稳态荧光探针法测定SDS表面活性剂胶束聚集数
稳态荧光探针法测定SDS表面活性剂胶束聚集数
陈红梅
【期刊名称】《广东化工》
【年(卷),期】2009(36)8
【摘要】以芘作为荧光探针,十六烷基氯化吡啶(CPC)作为猝灭剂,根据芘的荧光强度之比随表面活性剂水溶液浓度的变化,测定十二烷基硫酸钠(SDS)的临界胶束浓度(cmc),测定值与文献值较一致.并用外推法得到SDS的胶束聚集数(n).文章提供了一种简单可行的实验方法了来计算和评价胶束的基本特性.
【总页数】2页(P219-220)
【作者】陈红梅
【作者单位】武汉工业学院,化学与环境工程系,湖北,武汉,430023
【正文语种】中文
【中图分类】O65
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4.稳态荧光探针法测定松香基季铵盐Gemini表面活性剂胶束聚集数 [J], 蒋福宾;
曾华辉;杨正业;李俊杰;宋宝玲
5.一类新的表面活性剂——长链烷基三苯基鏻——Ⅵ.稳态荧光猝灭法测定十二烷基三苯基溴化鏻胶束的平均簇集数 [J], 江云宝;许金钩;陈国珍
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聚二甲基硅氧烷芯片自由酶反应器检测葡萄糖
聚二甲基硅氧烷芯片自由酶反应器检测葡萄糖作者:仲海燕周洁余晓冬陈洪渊【摘要】应用电泳中介微分析(EMMA)技术,构建聚二甲基硅氧烷(PDMS)芯片自由酶反应器,在线检测葡萄糖(Glu),在十字形的芯片通道上,采用自制的碳纤维微电极检测葡萄糖氧化酶(GOD)催化氧化Glu生成的H2O2,并对检测电位、GOD浓度、GOD进样时间、分离电压等参数进行了优化,测定了该自由酶反应器的线性范围和检出限,考察了其重现性及稳定性。
结果表明,此自由酶反应器制作方便,操作简单,重现性好,Glu浓度在0.1~20 mmol/L之间有较好的线性关系(r=0.997),检出限为19.8 μmol/L(S/N=3)。
【关键词】自由酶反应器;葡萄糖;电泳中介微分析;聚二甲基硅氧烷芯片The Key Lab of Analytical Chemistry for Life Science, Ministry of Education, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093)Abstract Microfluidic enzyme based reactor could be used to detect the biomolecules with high sensitivity and selectivity. Based on the electrophoretic mediated microanalysis (EMMA) method, a new kind of free enzyme based poly(dimethylsiloxane) (PDMS) microchip was developed to detect glucose (Glu). On line catalysis reaction of Glu by glucose oxidase (GOD) was carriedout on the chip. The product H2O2 was detected using single carbon fibre cylindrical electrode. Factors influencing the separation and detection, such as detection potential, GOD concentration, GOD injection time and separation voltage, were investigated and optimized. Results showed that the peak current had a good linear relationship with Glu concentration in the range of 0.1-20 mmol/L (R=0.997). The detection limit of Glu was 19.8 μmol/L (S/N=3). In addition, the PDMS enzyme based reactor had long term stability and excellent reproducibility (RSD=2.02%, n=10). It was easy to fabricate and operate, which showed great potential application in bioanalysis.Keywords Free enzyme based microchip; Glucose; Electrophoretic mediated microanalysis; Poly(dimethylsiloxane)1 引言近年来,基于酶催化反应的微流控芯片备受关注。
一种非动物源低分子量肝素及其制备方法与应用[发明专利]
专利名称:一种非动物源低分子量肝素及其制备方法与应用专利类型:发明专利
发明人:刘纯慧,唐凤琰,王亚利,马亚卿,张桂姣
申请号:CN202010042666.9
申请日:20200115
公开号:CN111154819A
公开日:
20200515
专利内容由知识产权出版社提供
摘要:本发明涉及一种非动物源低分子量肝素及其制备方法与应用。
本发明的制备方法包括一步化学法与多种酶催化法有序进行的步骤:提取胞外多糖K5CPS,将其化学法N‑脱乙酰化/N‑硫酸化修饰后,再经肝素酶Ⅲ在缓冲溶液中部分解聚;所得到的低分子量产物依次进行酶法C‑5差向异构
化/2‑O‑硫酸化修饰、6‑O‑硫酸化修饰、3‑O‑硫酸化修饰,得到具有抗FXa和FIIa活性的产物。
本发明提供了制备具有肝素典型结构特征和显著抗凝活性的低分子量产物的新方法,该方法的原料非动物来源、质量可控、污染风险低,采用的反应条件温和、高效,所得产物易分离,能够实现结构均一性好、抗凝活性强的非动物源低分子量肝素的规模化制备。
申请人:山东大学
地址:250012 山东省济南市历下区文化西路44号
国籍:CN
代理机构:济南金迪知识产权代理有限公司
代理人:杨磊
更多信息请下载全文后查看。
虎杖苷负离子质谱 -回复
虎杖苷负离子质谱-回复虎杖苷(tuberoside)是一种二萜类化合物,存在于很多植物中,如虎杖(Polygonum multiflorum Thunb.)和紫手参(Polygala tenuifolia Willd.)等。
它具有多种药理作用,例如改善认知功能、抗抑郁、抗氧化和抗炎等。
为了更好地研究虎杖苷及其降解产物,科学家们采用负离子质谱技术进行了深入的分析。
负离子质谱(Negative ion mass spectrometry)是一种常用的分析技术,可以用来研究化合物中的负离子,能够提供有关其分子结构和组成的信息。
虎杖苷的负离子质谱可以为研究者提供详细的分子信息,从而在化学和生物医学领域中发挥重要作用。
负离子质谱的步骤如下:1. 样品制备:首先,需要从虎杖苷中提取化合物。
常用的提取方法包括超声波提取、蒸馏提取和溶剂抽提等。
提取后的样品通常需要进行纯化和浓缩,以得到高质量的化合物。
2. 负离子化:为了在负离子质谱仪中进行分析,需要将虎杖苷样品负离子化。
常用的负离子化方法包括电喷雾(ESI)和化学游离法(CI)等。
这些方法可以将样品中的分子转变为负离子,并帮助分析师更好地观察和分析。
3. 质谱分析:将负离子化的虎杖苷样品导入负离子质谱仪进行分析。
负离子质谱仪通常由离子源、质量选择器和检测器等部分组成。
在负离子质谱仪中,样品中的负离子被加速并分离,然后根据它们的质量-荷质比进行检测和记录。
4. 数据分析:负离子质谱仪将提供一系列峰,每个峰代表一个特定的负离子化合物。
可以通过质谱图来确定分子的相对分子质量,以及其与其他化合物之间的相对丰度和组成关系。
进一步分析可以通过与数据库中的已知质谱图进行比对,以确定虎杖苷及其降解产物的结构和组成。
最后,通过对虎杖苷负离子质谱数据的深入分析,可以得到关于其分子结构和组成的详细信息。
这些信息对于进一步研究虎杖苷的药理作用以及其他相关化合物的分析具有重要意义。
此外,负离子质谱技术还可以应用于食品安全检测、环境污染监测等领域,为科学家提供更多的分析手段和数据支持。
乳酸氧化酶分子量
乳酸氧化酶分子量摘要:1.乳酸氧化酶的概述2.乳酸氧化酶的分子量3.乳酸氧化酶分子量的意义4.乳酸氧化酶分子量的测定方法5.乳酸氧化酶分子量在不同领域的应用正文:一、乳酸氧化酶的概述乳酸氧化酶(Lactate Dehydrogenase,简称LDH)是一种广泛存在于生物体内的酶,主要参与乳酸的生成与分解过程。
乳酸氧化酶可以催化乳酸(L-lactate)和丙酮酸(pyruvate)之间的相互转化,这是一种重要的生物化学反应。
二、乳酸氧化酶的分子量乳酸氧化酶的分子量因物种和组织来源的不同而有所差异。
一般来说,乳酸氧化酶的分子量约为135-140kDa。
这是由于乳酸氧化酶是由四个亚基组成的四聚体,每个亚基的分子量约为30-40kDa。
三、乳酸氧化酶分子量的意义乳酸氧化酶分子量的测定对于研究乳酸氧化酶的结构、功能、活性以及应用具有重要意义。
通过测定乳酸氧化酶分子量,可以了解乳酸氧化酶的聚合状态、亚基组成等信息,从而为进一步研究提供依据。
四、乳酸氧化酶分子量的测定方法乳酸氧化酶分子量的测定方法有多种,常用的有SDS-PAGE 法、高效液相色谱法(HPLC)等。
这些方法可以准确测定乳酸氧化酶的分子量,为研究提供可靠的数据。
五、乳酸氧化酶分子量在不同领域的应用乳酸氧化酶分子量在生物学、医学、食品工业等领域具有广泛的应用。
在生物学研究中,通过测定乳酸氧化酶分子量,可以研究乳酸氧化酶的结构和功能;在医学领域,乳酸氧化酶分子量的测定有助于诊断某些疾病,如心肌梗死、肿瘤等;在食品工业中,乳酸氧化酶分子量的测定可以评价食品的品质和加工过程中的变化。
综上所述,乳酸氧化酶分子量对于研究乳酸氧化酶的结构、功能、活性以及应用具有重要意义。
聚钙羧酸膜修饰电极对亚甲基蓝的检测研究
聚钙羧酸膜修饰电极对亚甲基蓝的检测研究
蔡素婷
【期刊名称】《福建分析测试》
【年(卷),期】2015(000)001
【摘要】制备了聚钙羧酸膜修饰玻碳电极,采用循环伏安法和方波伏安法研究了亚甲基蓝在修饰电极上的电化学行为,优化了包括支持电解质、缓冲液pH等测定条件。
建立了亚甲基蓝测定的新方法。
结果表明:该方法灵敏度高、检测范围宽,可用于水产品中亚甲基蓝残留的测定。
【总页数】5页(P6-10)
【作者】蔡素婷
【作者单位】晋江市质量计量检测所,福建晋江 362200
【正文语种】中文
【相关文献】
1.电催化测定烟酰胺腺嘌呤二核苷酸——以聚亚甲基蓝一碳纳米管修饰电极作为工作电极 [J], 杨怀成;曾金祥;魏万之
2.多巴胺在聚亚甲基蓝/石墨烯修饰电极上的电化学行为研究 [J], 郭新美;王宗花;夏建飞;张菲菲;夏延致;李延辉
3.聚亚甲基蓝/纳米二氧化硅修饰电极的制备及其对NADH的电催化氧化 [J], 刘晓琴;唐洁
4.氧化锆/聚亚甲基蓝修饰电极检测CaMV35S启动子基因 [J], 张娜;徐基贵;谈胜福;汪丽;张克营;王聪;杨柳青
5.聚亚甲基蓝-电还原石墨烯修饰电极电化学检测药物芦丁的研究 [J], 王玉华;钱淼;李环;李得鑫;王成森
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非布司他荧光药物分子的合成及体外活性测试
非布司他荧光药物分子的合成及体外活性测试
汪绪龙;姜开元;刘洋;杨文宁;隋强
【期刊名称】《合成化学》
【年(卷),期】2022(30)6
【摘要】以2-(3-氰基-4-羟基苯基)-4-甲基-1,3-噻唑-5-羧酸乙酯为原料,经水解得到中间体10;以二甲基膦乙酸苄酯为原料,经Witting反应、氢化还原双键、环合及钯/碳催化氢化脱苄基4步反应,得到中间体BODIPY-FL;10与BODIPY-FL缩合得到非布司他荧光药物探针分子,其结构经^(1)H NMR,MS(ESI)表征。
体外生物活性测试结果显示:该荧光探针的IC_(50)为8.3 nM,说明接入荧光基团后探针分子与黄嘌呤氧化酶(XOD)之间的亲和力略有下降。
【总页数】6页(P488-493)
【作者】汪绪龙;姜开元;刘洋;杨文宁;隋强
【作者单位】上海工程技术大学化学化工学院;中国医药工业研究总院;北京中医药大学
【正文语种】中文
【中图分类】R914.5
【相关文献】
1.不同剂量非布司他与别嘌呤醇对高尿酸血症伴痛风患者可溶性细胞间黏附分子1影响的对比观察
2.水溶性荧光高分子乙烯基咔唑—丙烯酸共聚物的合成及荧光测试
3.降尿酸药物别嘌醇、非布司他和苯溴马隆的严重不良反应综述
4.小分子药物
索非布韦对牛病毒性腹泻病毒体外复制的影响5.非布司他片放大制备工艺及体外一致性评价
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蛋白印迹——western blot 实验流程及注意事项
蛋白印迹——Western blot实验流程及注意事项各种凝胶电泳方法可以直接了解蛋白的某些性质如迁移率、电荷、大小、丰度以至蛋白的亲疏水性等。
此外,特异染色方法可用于识别不同种类的蛋白质。
Western blot技术大大扩展了凝胶电泳后识别和鉴定蛋白的可能性。
这一技术首先将电泳分离后的蛋白质条带或斑点从凝胶介质转移到固相支持膜上,然后用特异性配体进行检测。
特异性抗体和凝集素等已被广泛应用检测印迹膜上的蛋白。
印迹技术还常常作为电泳分离后用化学方法(如蛋白氨基端序列分析等)鉴定蛋白的一个重要步骤。
一、聚丙烯酰胺凝胶电泳(Polyacrylamide gel electrophoresis, PAGE或SDS-PAGE)聚丙烯酰胺凝胶电泳简称为PAGE(Polyacrylamide gel electrophoresis),是以聚丙烯酰胺凝胶作为支持介质的一种常用电泳技术。
聚丙烯酰胺凝胶由单体丙烯酰胺和甲叉双丙烯酰胺聚合而成,聚合过程由自由基催化完成。
催化聚合的常用方法有两种:化学聚合法和光聚合法。
化学聚合以过硫酸铵(AP)为催化剂,以四甲基乙二胺(TEMED)为加速剂。
在聚合过程中,TEMED催化过硫酸铵产生自由基,后者引发丙烯酰胺单体聚合,同时甲叉双丙烯酰胺与丙烯酰胺链间产生甲叉键交联,从而形成三维网状结构。
PAGE根据其有无浓缩效应,分为连续系统和不连续系统两大类,连续系统电泳体系中缓冲液pH值及凝胶浓度相同,带电颗粒在电场作用下,主要靠电荷和分子筛效应。
不连续系统中由于缓冲液离子成分,pH,凝胶浓度及电位梯度的不连续性,带电颗粒在电场中泳动不仅有电荷效应,分子筛效应,还具有浓缩效应,因而其分离条带清晰度及分辨率均较前者佳。
不连续体系由电极缓冲液、浓缩胶及分离胶所组成。
浓缩胶是由AP催化聚合而成的大孔胶,凝胶缓冲液为pH6.7的Tris-HCL。
分离胶是由AP催化聚合而成的小孔胶,凝胶缓冲液为pH8.9 Tris-HCL。
2,5-二甲基-1,3,4-噻二唑激发态结构动力学的共振拉曼光谱研究
2,5-二甲基-1,3,4-噻二唑激发态结构动力学的共振拉曼光谱研究庞素梅;赵彦英【期刊名称】《浙江理工大学学报》【年(卷),期】2017(037)001【摘要】获得了2,5-二甲基-1,3,4-噻二唑(DMETD)在乙腈、甲醇和水中的紫外吸收光谱以及三种溶剂中不同激发波长下的共振拉曼光谱,并结合含时密度泛函TD-B3 LYP/6-311++G(d,p)计算对DMETD的短时结构动力学进行了研究。
通过固态的 FT-Raman、FT-IR 光谱和 DFT 计算指认了 DMETD 在溶剂中的共振拉曼光谱,并对DMETD共振拉曼光谱的强度模式进行了分析。
结果表明DMETD在气相和溶剂中的跃迁主体都是π→π∗。
溶剂模型计算发现溶剂的极性对拉曼强度无影响。
这样,DMETD在 Franck-Condon区域活性振动模大致可以指认为7个基频v8,v12,v15,v17,v22,v23和 v27以及它们的倍频和组合频,C=N和 N-N伸缩振动占据了对拉曼强度的主要部分,说明 S2激发态结构动力学也主要沿这两个坐标进行。
%The ultraviolet absorption spectra and resonance Raman spectra of 2,5-dimethyl-1,3,4-thiadiazole (DMETD)were obtained inacetonitrile,methanol and water.Short-time structural dynamics of DMETD was studied in combination of density functional theory TD-B3 LYP/6-3 1 1 ++G (d,p ). Resonance Raman spectra of DMETD in the solvents were identified through solid-state FT-Raman,FT-IR spectrum and DFT calculation.Besides,the intensity mode of Resonance Raman spectra of DMETD was analyzed.The results show that transition subj ect of DMETD ingas phase an d solvents isχ→χ∗.Solvent model calculation indicates that the polarity of solvents has no influence on Raman intensity.Thus,the active vibrational mode of DMETD in the Franck-Condon region can be identified as 7 fundamental frequencies (v8 ,v12 ,v15 ,v17 ,v22 ,v23 andv27 ),their frequency doubling and combined frequencies.C=N and N-N stretching vibrations occupy the main part of Raman intensity.This indicates that S2 excited state structural dynamics is mainly along the two coordinates.【总页数】6页(P127-132)【作者】庞素梅;赵彦英【作者单位】浙江理工大学理学院化学系,杭州 310018;浙江理工大学理学院化学系,杭州 310018【正文语种】中文【中图分类】O643.12【相关文献】1.含苯并哒嗪酮结构的2,5-二取代-1,3,4-噻二唑衍生物的超声波辐射合成及表征 [J], 张宇航2.以2,5-二巯基-1,3,4噻二唑(I)为桥连配体的二维镉(Ⅱ)配合物的制备与晶体结构[J], 王文祥;赵红3.6-N,N-二甲基腺嘌呤激发态结构动力学的共振拉曼光谱 [J], 刘崇;杜蕊;赵彦英;王惠钢;郑旭明4.2,5-二-(3,5-二甲基吡唑-4-巯基)-1,3,4-噻二唑-Cd(Ⅱ)配合物的合成、晶体结构及其抑菌活性 [J], 汪志勇; 梁一龙; 孙乐涛; 余昌金; 杨先炯; 张奇龙5.更正——2,5-二乙氧甲酰氨基-1,3,4-噻二唑超分子化合物的合成和晶体结构 [J], 林奇;张有明;魏太保;高黎明因版权原因,仅展示原文概要,查看原文内容请购买。
鼠尾草酸对葡聚糖硫酸钠诱导的小鼠溃疡性结肠炎的改善作用
鼠尾草酸对葡聚糖硫酸钠诱导的小鼠溃疡性结肠炎的改善作用焦鑫鑫;许敏;吴华;刘梓萱;肖俊松【期刊名称】《现代食品科技》【年(卷),期】2024(40)3【摘要】研究了鼠尾草酸(CA)对葡聚糖硫酸钠(DSS)诱导的小鼠溃疡性结肠炎(UC)的改善作用。
小鼠自由饮用含3%DSS的蒸馏水,连续7 d造模。
将60只小鼠随机分为5组:空白对照组(CK)、DSS模型组(DSS)、鼠尾草酸低剂量组(CAL)、鼠尾草酸高剂量组(CAH)、美沙拉嗪组(PC)。
通过小鼠体质量变化、疾病活动指数(DAI)评分、结肠组织病理学和肠道通透性变化评估CA对UC小鼠的干预作用。
通过测定结肠组织髓过氧化物酶(MPO)活性、超过氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、紧密连接蛋白ZO-1和Occludin的表达及肠道菌群组成的变化探讨可能的影响机制。
与DSS组相比,CA干预降低了UC小鼠的质量损失和DAI评分、改善了结肠组织病理损伤。
同时,CAL和CAH组结肠组织MPO活性显著降低、MDA含量分别降低了13.75%、70.00%(P<0.05),SOD活性分别升高了6.12倍、9.62倍(P<0.05),肠道通透性显著降低、ZO-1和Occludin蛋白的表达得到恢复。
50 mg/kg mb的CA灌胃提高了厚壁菌门和拟杆菌门的丰度比值,恢复了DSS诱导的UC小鼠中Akkermansia等有益菌属的丰度下降,降低了Alistipes等有害菌属的相对丰度。
CA对UC具有良好的改善作用,其机制可能与降低氧化应激水平、保护肠屏障和调控肠道微生物组成有关。
【总页数】10页(P18-27)【作者】焦鑫鑫;许敏;吴华;刘梓萱;肖俊松【作者单位】北京工商大学化学与材料工程学院;北京市食品添加剂工程技术研究中心(北京工商大学)【正文语种】中文【中图分类】R28【相关文献】1.鱼腥草多酚对葡聚糖硫酸钠(DSS)诱导小鼠溃疡性结肠炎的改善作用2.石榴汁对葡聚糖硫酸钠诱导小鼠溃疡性结肠炎的作用3.乌鸡肽对葡聚糖硫酸钠诱导的溃疡性结肠炎小鼠的保护作用4.黄芩素对葡聚糖硫酸钠诱导的溃疡性结肠炎小鼠的改善作用5.山姜素对葡聚糖硫酸钠诱导小鼠溃疡性结肠炎的保护作用及其作用机制研究因版权原因,仅展示原文概要,查看原文内容请购买。
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Inhibitors, Agonists, Screening LibrariesSafety Data Sheet Revision Date:May-24-2017Print Date:May-24-20171. PRODUCT AND COMPANY IDENTIFICATION1.1 Product identifierProduct name :Mosapride (citrate)Catalog No. :HY-B0189ACAS No. :112885-42-41.2 Relevant identified uses of the substance or mixture and uses advised againstIdentified uses :Laboratory chemicals, manufacture of substances.1.3 Details of the supplier of the safety data sheetCompany:MedChemExpress USATel:609-228-6898Fax:609-228-5909E-mail:sales@1.4 Emergency telephone numberEmergency Phone #:609-228-68982. HAZARDS IDENTIFICATION2.1 Classification of the substance or mixtureGHS Classification in accordance with 29 CFR 1910 (OSHA HCS)Acute toxicity, Oral (Category 4),H302Acute aquatic toxicity (Category 1),H400Chronic aquatic toxicity (Category 1),H4102.2 GHS Label elements, including precautionary statementsPictogramSignal word WarningHazard statement(s)H302 Harmful if swallowed.H410 Very toxic to aquatic life with long lasting effects.Precautionary statement(s)P264 Wash skin thoroughly after handling.P270 Do not eat, drink or smoke when using this product.P273 Avoid release to the environment.P301 + P312 IF SWALLOWED: Call a POISON CENTER or doctor/ physician if you feel unwell.P330 Rinse mouth.P391 Collect spillage.P501 Dispose of contents/ container to an approved waste disposal plant.2.3 Other hazardsNone.3. COMPOSITION/INFORMATION ON INGREDIENTS3.1 SubstancesSynonyms:TAK⁻370 citrate; AS⁻4370 citrateFormula:C27H33ClFN3O10Molecular Weight:614.02CAS No. :112885-42-44. FIRST AID MEASURES4.1 Description of first aid measuresEye contactRemove any contact lenses, locate eye-wash station, and flush eyes immediately with large amounts of water. Separate eyelids with fingers to ensure adequate flushing. Promptly call a physician.Skin contactRinse skin thoroughly with large amounts of water. Remove contaminated clothing and shoes and call a physician.InhalationImmediately relocate self or casualty to fresh air. If breathing is difficult, give cardiopulmonary resuscitation (CPR). Avoid mouth-to-mouth resuscitation.IngestionWash out mouth with water; Do NOT induce vomiting; call a physician.4.2 Most important symptoms and effects, both acute and delayedThe most important known symptoms and effects are described in the labelling (see section 2.2).4.3 Indication of any immediate medical attention and special treatment neededTreat symptomatically.5. FIRE FIGHTING MEASURES5.1 Extinguishing mediaSuitable extinguishing mediaUse water spray, dry chemical, foam, and carbon dioxide fire extinguisher.5.2 Special hazards arising from the substance or mixtureDuring combustion, may emit irritant fumes.5.3 Advice for firefightersWear self-contained breathing apparatus and protective clothing.6. ACCIDENTAL RELEASE MEASURES6.1 Personal precautions, protective equipment and emergency proceduresUse full personal protective equipment. Avoid breathing vapors, mist, dust or gas. Ensure adequate ventilation. Evacuate personnel to safe areas.Refer to protective measures listed in sections 8.6.2 Environmental precautionsTry to prevent further leakage or spillage. Keep the product away from drains or water courses.6.3 Methods and materials for containment and cleaning upAbsorb solutions with finely-powdered liquid-binding material (diatomite, universal binders); Decontaminate surfaces and equipment by scrubbing with alcohol; Dispose of contaminated material according to Section 13.7. HANDLING AND STORAGE7.1 Precautions for safe handlingAvoid inhalation, contact with eyes and skin. Avoid dust and aerosol formation. Use only in areas with appropriate exhaust ventilation.7.2 Conditions for safe storage, including any incompatibilitiesKeep container tightly sealed in cool, well-ventilated area. Keep away from direct sunlight and sources of ignition.Recommended storage temperature:Powder-20°C 3 years4°C 2 yearsIn solvent-80°C 6 months-20°C 1 monthShipping at room temperature if less than 2 weeks.7.3 Specific end use(s)No data available.8. EXPOSURE CONTROLS/PERSONAL PROTECTION8.1 Control parametersComponents with workplace control parametersThis product contains no substances with occupational exposure limit values.8.2 Exposure controlsEngineering controlsEnsure adequate ventilation. Provide accessible safety shower and eye wash station.Personal protective equipmentEye protection Safety goggles with side-shields.Hand protection Protective gloves.Skin and body protection Impervious clothing.Respiratory protection Suitable respirator.Environmental exposure controls Keep the product away from drains, water courses or the soil. Cleanspillages in a safe way as soon as possible.9. PHYSICAL AND CHEMICAL PROPERTIES9.1 Information on basic physical and chemical propertiesAppearance White to off-white (Solid)Odor No data availableOdor threshold No data availablepH No data availableMelting/freezing point No data availableBoiling point/range No data availableFlash point No data availableEvaporation rate No data availableFlammability (solid, gas)No data availableUpper/lower flammability or explosive limits No data availableVapor pressure No data availableVapor density No data availableRelative density No data availableWater Solubility No data availablePartition coefficient No data availableAuto-ignition temperature No data availableDecomposition temperature No data availableViscosity No data availableExplosive properties No data availableOxidizing properties No data available9.2 Other safety informationNo data available.10. STABILITY AND REACTIVITY10.1 ReactivityNo data available.10.2 Chemical stabilityStable under recommended storage conditions.10.3 Possibility of hazardous reactionsNo data available.10.4 Conditions to avoidNo data available.10.5 Incompatible materialsStrong acids/alkalis, strong oxidising/reducing agents.10.6 Hazardous decomposition productsUnder fire conditions, may decompose and emit toxic fumes.Other decomposition products - no data available.11.TOXICOLOGICAL INFORMATION11.1 Information on toxicological effectsAcute toxicityClassified based on available data. For more details, see section 2Skin corrosion/irritationClassified based on available data. For more details, see section 2Serious eye damage/irritationClassified based on available data. For more details, see section 2Respiratory or skin sensitizationClassified based on available data. For more details, see section 2Germ cell mutagenicityClassified based on available data. For more details, see section 2CarcinogenicityIARC: No component of this product present at a level equal to or greater than 0.1% is identified as probable, possible or confirmed human carcinogen by IARC.ACGIH: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by ACGIH.NTP: No component of this product present at a level equal to or greater than 0.1% is identified as a anticipated or confirmed carcinogen by NTP.OSHA: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by OSHA.Reproductive toxicityClassified based on available data. For more details, see section 2Specific target organ toxicity - single exposureClassified based on available data. For more details, see section 2Specific target organ toxicity - repeated exposureClassified based on available data. For more details, see section 2Aspiration hazardClassified based on available data. For more details, see section 212. ECOLOGICAL INFORMATION12.1 ToxicityNo data available.12.2 Persistence and degradabilityNo data available.12.3 Bioaccumlative potentialNo data available.12.4 Mobility in soilNo data available.12.5 Results of PBT and vPvB assessmentPBT/vPvB assessment unavailable as chemical safety assessment not required or not conducted.12.6 Other adverse effectsNo data available.13. DISPOSAL CONSIDERATIONS13.1 Waste treatment methodsProductDispose substance in accordance with prevailing country, federal, state and local regulations.Contaminated packagingConduct recycling or disposal in accordance with prevailing country, federal, state and local regulations.14. TRANSPORT INFORMATIONDOT (US)This substance is considered to be non-hazardous for transport.IMDGUN number: 3077Class: 9Packing group: IIIEMS-No: F-A, S-FProper shipping name: ENVIRONMENTALLY HAZARDOUS SUBSTANCE, SOLID, N.O.S.Marine pollutant: Marine pollutant.IATAUN number: 3077Class: 9Packing group: IIIProper shipping name: Environmentally hazardous substance, solid, n.o.s.15. REGULATORY INFORMATIONSARA 302 Components:No chemicals in this material are subject to the reporting requirements of SARA Title III, Section 302.SARA 313 Components:This material does not contain any chemical components with known CAS numbers that exceed the threshold (De Minimis) reporting levels established by SARA Title III, Section 313.SARA 311/312 Hazards:No SARA Hazards.Massachusetts Right To Know Components:No components are subject to the Massachusetts Right to Know Act.Pennsylvania Right To Know Components:No components are subject to the Pennsylvania Right to Know Act.New Jersey Right To Know Components:No components are subject to the New Jersey Right to Know Act.California Prop. 65 Components:This product does not contain any chemicals known to State of California to cause cancer, birth defects, or anyother reproductive harm.16. OTHER INFORMATIONCopyright 2017 MedChemExpress. The above information is correct to the best of our present knowledge but does not purport to be all inclusive and should be used only as a guide. The product is for research use only and for experienced personnel. It must only be handled by suitably qualified experienced scientists in appropriately equipped and authorized facilities. The burden of safe use of this material rests entirely with the user. MedChemExpress disclaims all liability for any damage resulting from handling or from contact with this product.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。