LovastatinPPT

4.Cultivation parameters a.pH(5-8) b.Aeration/Agitation:an efficient supply of DO is inevitably required for lovastatin production c.particle size(SSF) :a certain combination particle size and moisture content should considered of be
a)
secondary metabolites and enzymes are only produced by SSF
b)
solid substrates was a limiting factor for the production of lovastatin
c e l l u l o s e , h e m i c e l l u l o s e , a n d l i g n i n ,wheat bran(best solid substrate)
4.Strategies for lovastatin purification and detection

purification :ethyl acetate acetonitrile
Methanol and ethanol
supercritical CO2 (SC-CO2)

the extraction of lactone lovastatin:butyl acetate or isopropyl acetate
Lovastatin production: From molecular basis to industrial process optimization
Kelly C.L. Mulder , Flávia Mulinari , Octávio L. Franco , Maria S.F. Soares ,Beatriz S. Magalhães , Nádia S. Parachin

2.Lovastatin interactions in HGM-CoA reductase active sites
2.Lovastatin interactions in HGM-CoA reductase active sites
3.The lovastatin biosynthetic pathway
From a commercial standpoint, this review covers all the information available from patent databases covering all protected aspects regarding lovastatin bioproduction
1.Introduction

This review brings a complete overview of the mechanisms in which lovastatin inhibits active sites of HMG-CoA reductase,the genetic basis for lovastatin production, detection and quantification protocols, the different strain screening assays in addition to a complete vision on what has been done during optimization of cultivation conditions.
Screening for over-producing strains Fermentation mode and cultivation for optimized lovastatin production
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6.
1.Introduction
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The WHO reported that cardiovascular disease is the leading cause of death worldwide.


6.Fermentation mode and cultivation for optimized lovastatin production
1. Fermentation modes: solid state fermentation (SSF) and submerged fermentation(SmF).
Statins are the most widely used drugs for hypercholesterolemia treatment. The first reported statin, mevastatin, also known as compactin, was first discovered in 1976. Merck started a program for isolating new antihypercholesterolemic compounds ,it has been called lovastain.
detection: HPLC ESI-MS UV(238 nm) LC Capillary electrophoresis LC-MS

5.Screening for over-producing strains

the highest lovastatin production strains:A. terreus naturally random mutagenesis ,expose UV,extensive screening Strain engineering and cultivation optimization processes
c)
6.Fermentation mode and cultivation for optimized lovastatin production
2.SmF:batch and fed-batch(lactose or glycerol as carbon sources)
3.Medium composition:the C/N ratio,B-vitamins
6.Fermentation mode and cultivation for optimized lovastatin production
d.pellet morphology in SmF:lovastatin synthesis is likely to occur in the filamentous zone of the pellet and not in the central core. pellet morphology was related to agitation. f.temperature(range from 23 °C to 28 °C),biomass formation,inoculum age and size. 5.Biosynthesis of by-products It is known that A. terreus is a rich secondary metabolite producer and is able to synthesize a wide variety of by-products such as 6-methylsalicylic acid, pigment precursors, citrinin, sulochrin, asterric acid, and (+)-geodin.
Contents
1.
Introduction Lovastatin interactions in HGM-CoA reductase active sites The lovastatin biosynthetic pathway
2.
3.
4.
Strategies for lovastatin purification and detection
thanks
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1.Introduction
Statins can be produced via microbial or chemical synthesis. Among the ones produced via microbial synthesis,lovastatin is the most studied.