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鲁昂体育馆

鲁昂体育馆

项目名称_鲁昂体育馆 项目地点_法国 鲁昂 设计者_多米尼克·佩罗建筑师事务所 委托人_La Crea 建筑面积_17 000m 2 建设时间_2010-2012年 模型_ Andr é Morin / DPA / Adagp 摄影_ Axel Dahl, Georges Fessy, Jacques Refuveille / DPA / Adagp, Paris, 2013PROJECT NAME _ Rouen Sports Palace LOCATION _ Rouen, France ARCHITECT _Dominique Perrault Architecte FLOOR AREA _ 17 000m 2 CONSTRUCTION TIME _2010-2012 MODEL _ André Morin / DPA / Adagp PHOTOGRAPHY _ Axel Dahl, Georges Fessy, Jacques Refuveille / DPA / Adagp, Paris, 2013鲁昂体育馆ROUEN SPORTS PALACE有两个基底而没有顶点的金字塔a pyramid with two bases and no apex总平面图鲁昂肯达瑞纳体育馆位于塞纳河右岸,邻近鲁昂历史中心,与周边其他新设施(如散步道、h2o 科学探索中心、106当代音乐舞台、未来的Luciline区、第六大桥、Flaubert生态社区和新码头等)共同组成了鲁昂城市群重点再开发项目中至关重要的一环。

该设施集群位于重新开发的区内,独特的场地位置标志着该地区改善塞纳河沿岸的空间品质、创造新地区中心的决心。

肯达瑞纳占据了整个区块结构中得天独厚的场地位置,位于TEOR换乘站、76码头和塞纳河码头之间,意味着这一基础设施将成为新的城市开发项目成功与否的关键因素。

具有传统纪念价值的重点体育基础设施是城市肌理中最能够触发感情的一部分。

罗马全面战争军种中英文对照

罗马全面战争军种中英文对照

amazon_chariots 亚马逊战车armenian_legionaries 亚美尼亚步兵barb_archer_dacian 弓箭手barb_archer_scythian 弓箭手barb_archer_slave 弓箭手barb_ballistas_dacian 弩炮barb_berserker_german 日耳曼狂战士barb_british_general_briton精锐酋长卫队barb_cavalry_dacian 蛮族骑兵barb_cavalry_gaul 蛮族骑兵barb_cavalry_german 蛮族骑兵barb_cavalry_scythian 蛮族骑兵barb_cavalry_slave 蛮族骑兵barb_chariot_heavy_briton不列颠重型战车barb_chariot_heavy_slave叛军重型战车barb_chariot_light_briton不列颠轻型战车barb_chieftain_cavalry_dacian蛮族精锐酋长卫队barb_chieftain_cavalry_early_dacia n 蛮族酋长卫队barb_chieftain_cavalry_early_gaul 蛮族酋长卫队barb_chieftain_cavalry_early_germ an 蛮族酋长卫队barb_chieftain_cavalry_early_scyth ian 蛮族酋长卫队barb_chieftain_cavalry_early_slave 蛮族酋长卫队barb_chieftain_cavalry_gaul蛮族精锐酋长卫队barb_chieftain_cavalry_german蛮族精锐酋长卫队barb_horse_archers_scythian西徐亚弓骑兵barb_horse_archers_slave叛军弓骑兵barb_infantry_briton 不列颠步兵barb_infantry_dacian 达西亚步兵barb_infantry_gaul 高卢步兵barb_infantry_slave 叛军步兵barb_noble_cavalry_dacian 贵族骑兵barb_noble_cavalry_gaul 贵族骑兵barb_noble_cavalry_german 贵族骑兵barb_noble_cavalry_slave 贵族骑兵barb_noble_horse_archers_scythia n 西徐亚贵族弓骑兵barb_onagers_dacian 石弩barb_onagers_scythian 石弩barb_peasant_briton 蛮族农人barb_peasant_dacian 蛮族农人barb_peasant_gaul 蛮族农人barb_peasant_german 蛮族农人barb_peasant_scythian 蛮族农人barb_peasant_slave 蛮族农人barb_peltast_gaul 标枪战士barb_peltast_german 标枪战士barb_rebel_general 叛军将军卫队barb_scythian_chieftain_cavalry_sc ythian 蛮族精锐酋长卫队barb_slinger_briton 投石兵barb_wardogs_briton 战獒barb_wardogs_dacian 战獒barb_wardogsgaul 战獒barb_wardogsgerman 战獒barb_wardogs_scythian 战獒barb_warguard_briton不列颠精锐武士barb_warguard_dacian达西亚精锐武士barb_warguard_gaul高卢精锐武士barbarian_civ_peasant 蛮族成员barbarian_female_peasant 蛮族成员bronze_shield 本都铜盾长枪兵bull_warriors 公牛战士carthaginian_archer 弓箭手carthaginian_cavalry 圆盾骑兵carthaginian_city_militia 城镇民兵carthaginian_civ_peasant 迦太基公民carthaginian_elephant_african战象carthaginian_elephant_african_cat aphract 披甲战象carthaginian_elephant_forest大象carthaginian_generals_cavalry精锐将军卫队carthaginian_generals_cavalry_ear ly 将军卫队carthaginian_heavy_infantry 布匿步兵carthaginian_heavy_onagers 巨型石弩carthaginian_infantry 伊比利亚步兵carthaginian_medium_cavalry长盾骑兵carthaginian_medium_infantry利比亚枪兵carthaginian_onagers 石弩carthaginian_peasant 农人carthaginian_peltast 标枪兵carthaginian_royal_cavalry 圣团骑兵carthaginian_sacred_band 圣团步兵carthaginian_slinger 投石兵carthaginian_warhounds 战獒cheat_oliphants 猛犸战象druids_briton 不列颠德鲁伊教团druidsgaul 高卢德鲁伊教团druids_slave 叛军德鲁伊教团east_archer 弓箭手east_camel_cataphract 铁甲骆驼骑兵east_cappodocian_cavalry卡帕多西亚骑兵east_cataphract_archer铁甲弓骑兵east_cavalry 本都轻骑兵east_chariot 卷镰战车east_chariot_archer 弓箭战车east_elephant_african 战象east_generals_cavalry东方精锐将军卫队east_generals_cavalry_early东方将军卫队east_heavy_cataphract 铁甲骑兵east_heavy_cavalry 本都重骑兵east_heavy_infantry 重装长枪兵east_hillmen 丘陵兵east_hoplite 本都长枪兵east_horse_archer 弓骑兵east_infantry 东方步兵east_onager 石弩east_peasant 农人east_peltast 标枪兵east_pontic_generals东方精锐将军禁卫队east_slinger 投石兵eastern_civ_peasant 东方工人eastern_female_peasant 东方工人eastern_rebel_generals 叛军将军卫队eat_persian_cavalry 波斯弓骑兵egyptian_archer 弓箭手egyptian_archer_elite 法老天选弓箭手egyptian_camel_archers 骆驼弓骑兵egyptian_cavalry 沙漠骑兵egyptian_chariot 埃及战车egyptian_chariot_archer 埃及弓箭战车egyptian_civ_peasant 埃及工人egyptian_cleruch 尼罗河骑兵egyptian_elite_guards 法老禁卫军egyptian_female_peasant 埃及工人egyptian_general_early 埃及将军卫队egyptian_generals_bodyguard埃及精锐将军卫队egyptian_heavy_onagers 巨型石弩egyptian_infantry 沙漠斧头兵egyptiannile_cavalry 努比亚骑兵egyptiannile_infantry 尼罗河长枪兵egyptiannubian_spearmen 努比亚长枪兵egyptian_onagers 石弩egyptian_peasant 农人egyptian_peltast 标枪兵egyptian_slingers 投石兵generic_rebel_general 叛军将军卫队gothic_cavalry_german 哥特骑兵greek_archer 弓箭手greek_ballistas 弩炮greek_bastarnae 巴斯塔奈战士greek_cavalry 标枪骑兵greek_civ_peasant 希腊公民greek_elephant_african 战象greek_elephant_armoured 披甲战象greek_elephant_small 大象greek_female_peasant 希腊公民greek_generalsguard_cavalry精锐将军卫队greek_generalsguard_cavalry_earl y 将军卫队greek_heavy_cavalry 马其顿骑兵greek_heavy_onagers 巨型石弩greek_heavy_peltast 重装标枪兵greek_hoplite 希腊步兵greek_hoplite_elite 希腊重步兵greek_hoplite_militia 希腊民兵greek_hoplite_spartan 斯巴达重步兵greek_legionary 银盾步兵greek_levy_pikemen 应征长枪兵greek_medium_cavalry 希腊骑兵greek_onagers 石弩greek_peasant 农人greek_peltast 标枪兵greek_pigs 火猪greek_pikemen 方阵长枪兵greek_royal_cavalry 皇家骑兵greek_royal_pikemen马其顿皇家长枪兵greek_scythed_chariots 卷镰战车greek_silver_shields 银盾长枪兵greek-Light_lancer 马其顿轻骑兵head_hunting_maidens_scythian 西徐亚女猎骑judean_zealots 犹太狂热分子merc_arab_cavalry 阿拉伯雇佣骑兵merc_balearic_slingers巴利阿里投石兵merc_barbarian_cavalry蛮族雇佣骑兵merc_barbarian_infantry 蛮族佣兵merc_bastarnae 巴斯塔奈佣兵merc_bedouin 贝多因骆驼轻骑兵merc_bedouin_archers贝多因骆驼弓骑兵merc_cilician_pirate 西里西亚海盗merc_cretan_archers 克里特弓箭手merc_eastern_infantry 东方佣兵merc_elephants 雇佣战象merc_greek_hoplites 雇佣长枪兵merc_illyrian 伊利里亚佣兵merc_libyan_infantry 利比亚佣兵merc_numidian_cavalry努米底亚雇佣骑兵merc_peltast 雇佣标枪兵merc_rhodian_slingers 罗德斯投石兵merc_samnite 萨莫奈佣兵merc_sarmatian_cavalry萨尔马提亚雇佣骑兵merc_spanish_infantry 西班牙佣兵merc_thracian 色雷斯佣兵naked_fanatics_dacian达西亚狂热战士naked_fanatics_gaul高卢狂热战士naked_fanatics_german日耳曼狂热战士naked_fanatics_slave 叛军狂热战士naked_fanatics_spain西班牙狂热战士naval_bireme 双列战船naval_boats 战船naval_corvus 天鸦舰naval_decere 七列战船naval_large_boats 大型战船naval_pirates 海盗船naval_quinquireme 五列战船naval_trireme 三列战船night_raiders_german 日耳曼夜袭团numidian_camel_riders努米底亚骆驼骑兵numidian_cavalry努米底亚标枪骑兵numidian_desert_warriors 沙漠步兵numidian_javelinmen 努米底亚标枪兵numidian_legionaries 努米底亚步兵roman_arcani 神隐突击团roman_archer 罗马弓箭兵roman_archer_auxillia罗马辅助弓箭兵roman_ballista 弩炮roman_cavalry_auxillia 罗马辅助骑兵roman_city_militia 城镇民兵roman_civ_peasant 罗马公民roman_female_peasant 罗马公民roman_hastati 罗马青年军roman_heavy_cavalry 罗马军团骑兵roman_heavy_onager 巨型石弩roman_incendiary_pigs 火猪roman_infantry_auxillia 罗马辅助步兵roman_legionary_cohort_i初期罗马军团步兵roman_legionary_cohort_ii罗马军团步兵roman_legionary_first_cohort_i 初期罗马鹰旗步兵roman_legionary_first_cohort_ii 罗马鹰旗步兵roman_light_cavalry 罗马轻骑兵roman_light_infantry 罗马辅助轻步兵roman_medium_cavalry 罗马骑兵roman_mirmillo_gladiator 角斗士roman_onager 石弩roman_peasant 农人roman_praetorian_cavalry罗马禁卫骑兵roman_praetorian_cohort_i罗马禁卫军roman_praetorian_cohort_urban_i 罗马精锐卫护军团roman_principes 罗马重步兵roman_repeating_ballistae 连弩炮roman_samnite_gladiator萨莫奈角斗士roman_scorpion 蝎弩roman_triarii 罗马精锐步兵roman_velite 罗马轻步兵roman_velite_gladiator维赖特角斗士roman_wardogs 战獒screeching_women_german日耳曼女战士scutarii 盾牌兵scythian_noble_women_scythian 西徐亚贵族女骑兵scythian_nobles_scythian西徐亚贵族骑兵thracian_generals_guard_cavalry 精锐将军卫队thracian_generals_guard_cavalry_ early 将军卫队warband_archer_dacian 精锐弓箭手warband_archer_german 精锐弓箭手warband_archer_scythian 精锐弓箭手warband_axe_german 日耳曼斧头兵warband_axe_scythian 西徐亚斧头兵warband_dhaxe_german日耳曼精锐斧头兵warband_falx_dacian 达西亚长刀兵warband_falx_thracian 色雷斯长刀兵warband_huntsman_gaul 丛林猎手warband_hurler_briton 掷头兵warband_spear_german 日耳曼长枪兵warband_sword_briton 不列颠武士warband_sword_gaul 高卢武士warband_woad_briton不列颠图腾战士。

各大联赛球队主场名称

各大联赛球队主场名称
维罗纳
克罗托内
埃济奥·希达球场(Ezio Scida)
克罗托内
佩斯卡拉
亚德里亚海球场(Adriatico)
佩斯卡拉
萨勒尼塔纳
阿勒切球场(Arechi)
萨勒尼塔纳
科莫
西尼加格利亚球场(Giuseppe Sinigaglia)
科莫
特拉纳
利伯纳蒂球场(Libero Liberati)
特拉纳
威尼斯
彭佐球场(Pierluigi Penzo)
索里亚
帕尔玛
塔尔迪尼球场(Ennio Tardini)
帕尔马
那不勒斯
圣保罗球场(San Paolo)
那不勒斯
锡耶纳
弗朗基球场(Artemio Franchi)
锡耶纳
佛罗伦萨
弗朗基球场(Artemio Franchi)
佛罗伦萨
亚特兰大
阿祖里球场(Atleti Azzuri d'Italia)
贝加莫
热那亚
路易吉·费拉里斯球场(Luigi Ferraris)
米德尔斯堡
朴茨茅斯
弗兰顿球场(FrattonPark)
朴茨茅斯
热刺
白鹿巷球场(WhiteHart Lane)
伦敦
布莱克本
伊伍德公园球场(EwoodPark)
布莱克本
曼城
城市体育场(TheCity of Manchester)
曼彻斯特
埃弗顿
古迪逊公园球场(GoodisonPark)
利物浦
伯明翰
圣安德鲁球场(St.Andrews)
德尔·弗伦德斯萨夫特球场(Stadion der Freundschaft)
科特布斯
法兰克福

布拉加市体育场_布拉加_葡萄牙

布拉加市体育场_布拉加_葡萄牙

布拉加市体育场,布拉加,葡萄牙BRAGA STADIUM, BRAGA, PORTUGAL, 2003建筑设计:苏托・莫拉建筑事务所ARCHITECTS: Souto Moura-Arquitectos1 1 外景/Exterior view布拉加市体育场位于克拉图山(Crasto )北坡的Dume 体育公园内。

选择这个地方作为基地可以避免新工程阻断山谷中的河流。

另一个选择是移到西边,依山而建,如同罗马的露天剧场一般。

如今,足球竞技是一项大型娱乐活动。

这个体育场只是设置了两面有座位的观众席。

体育场的屋顶看上去像长长的遮阳板(让人想起西扎为1998年世博会设计的葡萄牙馆),其实这个形式取自秘鲁古印加的桥。

体育场建筑两部分的看台坡度相同,高40m 。

这促使该建筑成为今后城市继续向北发展的起始点。

城市曾在20年前向南发展直至Carandá市场。

为了避免城市的衰落,目前那边的发展被中断了。

□(郑小东 译)The Braga Municipal Stadium is situated within the Dume Sports Park on the northern slope of Monte Crasto. The location was chosen in order to avoid making a dam along the water ’s edge in the valley.The alternative would have been to move it further to the west up against the hill, like a Roman amphitheatre.Nowadays football is big entertainment, hence the decision to have only two rows of seats. Initially the roof was to look like a long continuous visor (in reference to Siza ’s Portuguese Pavilion for EXPO 98),but it was eventually modelled on the Peruvian Inca bridges.With a height of 40 metres, the stadium is up against two squares with the same sloping. This enables the building to serve as an anchor point for any future development in the area as the city expands northwards. To the south, the same applied 20 years ago to the Carandá market. Today, it is being amputated to save it from succumbing to gangrene. □242 模型/Model3 总平面/Site plan4 草图/Sketch5业主/Client: 布拉加市府/Camara Municipal De Braga设计时间/Project: 2000.01建造时间/Construction: 2002.01/2003.12地点/Locality: Monte Crasto, Parque Norte, Braga建筑设计/Architects: Souto Moura-Arquitectos, Lda:Eduardo Souto de Moura, Carlo Nozza, Ricardo Merí,Enrique Penichet, Atsushi Hoshima, Diego Setien,Carmo Correia, Sérgio Koch, Joaquim Portela, LuisaRosas, Jorge Domingues, Adriano Pimenta, RicardoRosa Santos, Diogo Guimarães, JoséCarlos Mariano,João Queiroz e Lima, Tiago Coelho景观设计/Landscaping: Daniel Monteiro顾问/Consultants: Arup Associates-Dipesh Patel(Stadium Programme)技术设计/Engineers: AFASSOCIADOS-Projectos deEngenharia, SA技术协作/Coordination: Rui Furtado结构/Structures: Rui Furtado, Carlos Quinaz, Renato65 内景/Interior view6 首层平面/Ground floorBastos, Pedro Moas, Rui Oliveira, Rodrigo Andrade eCastro, Pedro Pacheco, Miguel Paula Rocha, AntónioAndré, João Dores, Sérgio Vale, Nuno Neves, RafaelGonãalves, Andreia Delfim, Miguel Braga, JoãoCoutinho, António Monteiro公共卫生设施/Public Health: Maria Elisa Parente,Joana Neves电气设备/Electrical Installations: António JoséRodrigues Gomes, António Ferreira, Luís Fernandes(RGA)机械设备/Mechanical Installations: José Silva Teixeira,Tiago Fernandes (RGA)安全/Safety: Christian Aoustin (GERISCO)土方/Excavation: Estevão Santana, João Burmester道路与基础设施/Roads and Infrastructures: EstevãoSantana, João Burmester顾问/Consultants: António Silva Cardoso(地质相关技术/Geotechnics); CG(地质相关技术/Geotechnics);7OVE ARUP & PARTNERS-Andrew Alisop/AndrewMinson(风力研究/Wind study); RWDI- Mark Hunter/Michael Soligo(刚体风力模型测试/rigid model windtests);DMI-Danish Maritim Institut- AageDamsghaard(弹性体风力模型测试/aeroelastic model Array wind tests); FEUP-Construction Institute- ElsaCaetano(动力学/Dynamics)最终缆线设计/Cables Final: TENSOTECI: MassimoMarini, Massimo Majowietcki; SOARES DA COSTA, SA:Luís Afonso, Diogo Santos现场监理/Site Inspection: Braga Municipal Council-Domsu: Manuel Afonso Basto, Carlos Amaral, LuísAlmeida, Filipe Vaz, Eduardo Leite, Paula Pereira,Cidália Rodrigues, Márcia Rodrigues, J. Rodrigues施工/Construction: 总开掘/General excavation:Aurélio Martins Sobreiro: Adérito Faneca; 山体固定/Slope stabilisation: ACE-ASSOC/TECNASOL: MárioDuarte, João F alcão; 构建安装/Structures,Installations: ACE- ASSOC/SOARES DA COSTA, SA; 饰面外观/Finishes and External: Lionel Correia; 施工/Works: Jorge Oliveira, Mário Duarte, Mário Pereira,Santos Costa建筑规模/Building Size: 30 000座/30 000 seats造价/Cost: 75 million Euros摄影/Photography: Luís Ferreira Alves87 施工中照片/Under construction8 二层平面/Floor 1 plan9109.10 内景/Interior view11 外景/Exterior view12.13剖面/Section111213。

Hockey-冰球

Hockey-冰球
Amateur ice hockey leagues began in the 1880s, and professional ice hockey originated around 1900.
Ice hockey was played for the first time in the Olympics in the Olympic Games of 1920.
• IJscolf, a game resembling colf on an ice-covered surface, was popular in the Low Countries between the Middle Ages and the Dutch Golden Age.
• In England, evidence of games of 'hockey on ice' (the name replaced "bandie ball"), played with a "bung" (a plug of cork or oak used as a stopper on a barrel) date back to the 1700s.
The belief that hockey was mentioned in a 1363 proclamation by King Edward III of England is based on modern translations of the proclamation, which was originally in Latin and explicitly forbade the games .
According to the Austin Hockey Association, the word "puck" derives from the Scots Gaelic puc or the Irish poc (to poke, punch or deliver a blow). "...The blow given by a hurler to the ball with his caman or hurley is always called a puck."

(人教版)选择性必修第一册:UNIT 3 FASCINATING PARKS(含解析)

 (人教版)选择性必修第一册:UNIT 3 FASCINATING PARKS(含解析)

选择性必修第一册UNIT 3 FASCINATING PARKSⅠ.阅读理解Guided Tours in the British MuseumAround the world in 70 minutes tourExplore some of the most famous objects on display at the Museum.See the details of the characterful Lewis Chessmen,surprise yourself at the ancient texts on the world-famous Rosetta Stone and get closer to more highlights from the collection. Time:11:30-12:40,13 Feb.2022-29 May 2022Price:£14 (Adults and children aged 12+)Life and death in ancient EgyptDiscover how people lived and died in ancient Egypt,and explore their hopes for the afterlife.From statues and models of daily life to wall paintings,investigate what tombs and burial goods tell us about the lives of ancient Egyptians.Time:09:00-10:00,13 Feb.2022-18 Dec.2022Price:£33;£30 (students,disabled visitors);£16.50 (Ages 5-15 years);Free for under 5sAn introduction to ChinaGain an insight into the development of Chinese history and culture from 5000 BC to the present day.Appreciate the exhibits featuring the Tang Dynasty tomb figures and the Ming Dynasty blue-and-white porcelain.Time:09:00-10:00,19 Feb.2022-17 Dec.2022Price:£33;£30 (students,disabled visitors);£16.50 (Ages 5-15 years);Free for under 5sAn introduction to ancient GreeceStart from the rise of the Greek city states to the empire of Alexander the Great.Enjoy some of the highlights of the collection,including sculptures from the Parthenon and the Mausoleum of Halikamassos,regarded as one of the Seven Wonders of theAncient World.Time:09:00-10:00,24 Feb.2022-26 June 2022Price:£33;£30 (students,disabled visitors);£16.50 (Ages 5-15 years);Free for under 5s1.When can you see the Rosetta Stone?A.At 9:30,15 Feb.B.At 11:30,26 May.C.At 12:00,6 June.D.At 12:30,17 Dec.2.What can people do in the tour of Life and death in ancient Egypt?A.Explore ancient tombs and burial goods.B.Witness the development of the country.C.Visit most famous galleries of the country.D.Appreciate sculptures from the Parthenon.3.How much should a couple with a child aged 6 pay for An introduction to China?A.£33.B.£49.5.C.£66.D.£82.5.With the development of apps,social media has seemingly revolutionized the way we travel.Now,young people primary travel inspiration comes from social media.Instead of choosing their destination from a selection in a catalogue at travel agent like before,they scroll(滚动) through online posts in search of that ideal location.When it comes to planning the perfect holiday,social media has become a huge factor for many young people today.Everybody wants to return home with an album full of artistic and “instagramable” photos that show off their trips.The best way to find that desired “post-able” holiday destination is now through social media itself.Whether it is viewing a friend’s recent travel post,or a photo directly from a travel account,social media inspires the desire to explore new places for so many of its users.Travel companies now also use social media as part of their own marketing strategies.The majority of airlines,tour companies,hotels and other travel-related businesses have their own Instagram,Facebook and Twitter pages to increase brand awareness.One of the leading marketing strategies is the use of the socialinfluences.Research shows that 70% of teens trust influences more than traditional celebrities—in 2018,1.6 billion were funnelled into sponsored influenced posts on Instagram alone;and 63 % of 18-24-year-old Instagram users follow travel influenced account.However,as with many aspects of social media,there were obvious disadvantages to this travel revolution.It could be argued that the obsession with documenting and sharing your travel adventures online distracts from being present and truly enjoying the experience.Our motives to travel seem to have shifted away from a strong desire to travel and have become more egocentric (自我为中心的),travelling now for the sake of likes and follows.This draws away from some of the most important elements of travel.In general,social media has revolutionized the way we travel,providing tonnes of inspiration and new opportunities.We should be grateful for the global awareness it has given us,and the insight into new places and different means of travel.At the same time,it is equally important not to allow the posts we see to affect our self-image or reflect on our own experiences any differently.4.How did people get their primary travel inspiration in the past?A.By appreciating photos from a travel account.B.By viewing friends’ recent travel post.C.By consulting a travel agent.D.By scrolling through online posts.5.Which of the following is right according to the text?ing Internet celebrities is a very effective marketing strategy.B.Most teenagers trust traditional celebrities more than social influences.C.The obsession with sharing travel experiences helps to enhance the travel experience.D.The travel inspiration of young people mainly comes from the tourism advertisements.6.What is the author’s attitude to the travel revolution?A.Skeptical.B.Objective.C.Worried.D.Disapproving.7.Which of the following is a suitable title for the text?A.Ways to enhance the travel experiences.B.Elements of modern travel.C.Inspiration and opportunities from social media.D.The impact of social media on travel.“It has nothing in common with anything else on the Bund(外滩),”said Ben Wood,the American architect behind Shanghai’s famous Xintiandi District,commenting on the Fosun Foundation after its completion in 2016.“The great thing about the Bund is that it’s grand.Perhaps a building that can stand out is needed here to show that a company is progressive.But it’s not grand.”For now,however,the Fosun Foundation—a new theatre—is proving popular with riverside tourists.Located in the Bund Finance Centre,the theatre was designed by cooperation of two world-known British design firms:Foster+Partners and Heatherwick Studio.The architectural highlight is the outward appearance—a golden,three-layered(层),steel bamboo curtain that hangs from the third floor.But what makes the architecture really unique is that it “dances”:repeatedly each day,the curtain slowly moves—opens and closes—with music.This visual element,combining East and West,looks like both an ancient Chinese crown and a Western harp(竖琴).The building’s ground floor entrance looks unclear,but once inside,you’ll see the space reveal a hall and café,and traditional Chinese theatres on the upper floors.Although there’re only four stories above ground,the building houses several music halls on its three underground floors.“The opportunity to make something new in this culturally historic location was extraordinary,” said Thomas Heatherwick,Heatherwick Studio founder.“We tried to make it an interesting addition to show Shanghai’s mix of modern and historic architecture.”The Fosun Foundation is only part of a bigger plan for this economic and culturalcentre in China.Along the Huangpu River,a massive waterfront art area is being built.Shanghai hopes to play a larger role in the global art industry in the coming years.What Shanghai makes of this new riverside project remains to be seen.But with the Fosun Foundation open to the public,the area’s rapid transformation is bringing up discussions in China’s arts scene and beyond.8.What did Ben Wood mainly talk about?A.How to keep the Bund area progressive.B.Why the Bund area doesn’t stand out.C.How to make the Fosun Foundation grand.D.Why the Fosun Foundation doesn’t fit the area.9.What makes the theatre unusual?A.The colour of its appearance.B.The material of the building.C.The movement of its curtain.D.The music of a different type.10.What is Shanghai’s bigger plan for the Bund area?A.To improve its influence in arts.B.To bring up more traditional buildings.C.To strengthen its economic role.D.To mix modern and historic elements.11.Which word best describes the author’s attitude to the plan?A.Worried.B.Pessimistic.C.Cautious.D.Confident.Ⅱ.七选五(2024·山西太原一模)Museums become the next stop many students want to visit at their leisure.__1__.However,the experience of visiting a museum could be quite disappointing if not done right, even for the experienced museum goers.__2____.No matter how much you’re enjoying yourself, many museums can takehours, days, or even weeks to fully explore.If you try to put it all in, you will get worn out to the point that you won’t really be seeing anything.Pick a few paintings and explore them in a detailed way, and your museum experience will be more relaxing. Have you ever started your visit by waiting in line in front of the museum for a few hours just to get in? And that’s the sad reality when visiting many of those world-famous museums.Thus, be smart to browse the instructions for visiting and book in advance online.__3__.What if you don’t like some museums?Then don’t visit them!I’m not a massive fan of parks, so I would hardly ever end up in any park while travelling.__4__.Do they have a nice collection of an art style you love? Just ask yourself and visit the museum website to see what the highlights are.There is nothing worse than impolite manners while visiting.We’ve all witnessed that guy being yelled at by the security for touching or taking flash photos.__5__.Otherwise, not only will you absolutely get embarrassed by security guards, but you can actually ruin artworks!All of these ground rules are often neglected by us.Bear them in mind before setting off, and you will enjoy your museum tour!A.It’s the same with the museumsB.But most of them do it all wrongC.If you haven’t,don’t let it be youD.That way you can have an easy accessE.The first step is to research how to enjoy everythingF.Sometimes you may sigh museum tiredness is a real thingG.Generally,there is no right or wrong way to visit a museum选择性必修第一册UNIT 3Ⅰ.【语篇解读】本文是应用文。

大运会徽章场馆介绍

大运会徽章场馆介绍

大运会徽章场馆介绍Grand Games Badge Venues.English Answer:The Grand Games Badge Venues are a collection of historical buildings and modern landmarks in the host city that serve as the primary locations for the Grand Games competitions. These venues have been meticulously selected to provide the optimal setting for each sport and discipline, ensuring the highest level of performance and entertainment for athletes and spectators alike.1. Grand Arena: The Grand Arena is the centerpiece of the Grand Games complex, hosting the opening and closing ceremonies as well as the track and field competitions. With a seating capacity of over 100,000, it is the largest indoor stadium in the world and promises to create an unforgettable atmosphere for these iconic events.2. Aquatic Center: The Aquatic Center is a state-of-the-art facility designed for swimming, diving, and water polo competitions. Its Olympic-sized pools and advanced filtration systems provide ideal conditions for these thrilling aquatic sports, while the retractable roof allows for both indoor and outdoor competitions.3. Velodrome: The Velodrome is a specially designed indoor cycling track that will host the cycling competitions. With its steeply banked curves and aerodynamic design, it offers the perfect environment for cyclists to push their limits and break records.4. Tennis Stadium: The Tennis Stadium features multiple courts set within a stunning outdoor complex. Its advanced lighting system and comfortable seating ensure optimal viewing conditions for both players and spectators, creating a vibrant and engaging atmosphere for tennis enthusiasts.5. Sports Pavilion: The Sports Pavilion is a multi-purpose venue that will host a variety of indoor sports,including basketball, volleyball, and gymnastics. Its flexible configuration allows for the seamless transition between different disciplines, accommodating the diverse needs of athletes and spectators.6. Archery Field: The Archery Field is a dedicated outdoor venue designed specifically for archery competitions. Its spacious layout and precise target ranges provide an ideal environment for archers to showcase their skill and precision.7. Beach Volleyball Arena: The Beach Volleyball Arenais a sandy paradise nestled in the heart of the Grand Games complex. With its dedicated courts and lively atmosphere, it will ignite the competitive spirit and provide thrilling matches for beach volleyball enthusiasts.8. BMX Track: The BMX Track is a challenging and exhilarating course designed for BMX racing. Its steep jumps and technical sections will put riders' skills to the test, creating a thrilling spectacle for spectators and adrenaline junkies alike.9. Shooting Range: The Shooting Range is a specialized facility designed for shooting competitions. Its isolated location and advanced safety systems ensure the highest level of precision and safety for shooters, while also providing a serene environment for focused performance.10. Equestrian Center: The Equestrian Center is a sprawling complex dedicated to equestrian sports. Its spacious arenas and well-maintained stables provide the perfect setting for dressage, show jumping, and other equestrian disciplines, showcasing the grace and athleticism of both horses and riders.Chinese Answer:盛大运动会徽章场馆。

求生之路2代码

求生之路2代码

联机难度选择:z_difficulty "Easy" 游戏难度简单z_difficulty "Normal" 正常z_difficulty "Hard" 困难z_difficulty "Impossible" 专家联机模式选择:map 地图名versus 对抗模式map 地图名realism 写实模式map 地图名survival 生存模式map 地图名scavenge 清道夫模式地图名:c1m1_hotel 1.死亡中心1旅馆c1m2_streets 1.死亡中心2街道c1m3_mall 1.死亡中心3购物中心c1m4_atrium 1.死亡中心4中厅c2m1_highway 1.黑色狂欢节1高速公路c2m2_fairgrounds 2.黑色狂欢节2游乐场c2m3_coaster 2.黑色狂欢节3过山车c2m4_barns 2.黑色狂欢节4谷仓c2m5_concert 2.黑色狂欢节5音乐会c3m1_plankcountry 3.沼泽激战1乡村c3m2_swamp 3.沼泽激战2沼泽c3m3_shantytown 3.沼泽激战3贫民窟c3m4_plantation 3.沼泽激战4种植园c4m1_milltown_a 4.暴风骤雨1密尔城c4m2_sugarmill_a 4.暴风骤雨2糖厂c4m3_sugarmill_b 4.暴风骤雨3逃离工厂c4m4_milltown_b 4.暴风骤雨4重返小镇c4m5_milltown_escape 4.暴风骤雨5逃离小镇c5m1_waterfront 5.教区1码头c5m2_park 5.教区2公园c5m3_cemetery 5.教区3墓地c5m4_quarter 5.教区4特区c5m5_bridge 5.教区5桥god 1 无敌模式god 0 退出无敌模式sv_infinite_ammo 1 无限弹药sv_infinite_ammo 0 关闭无限弹药impulse 10 所有武器和弹药give health 角色生命全满noclip 穿墙模式give物品名制造指定物品[附1]nb_blind 1 僵尸变瞎nb_delete_all 清除所有被感染的头目及幸存者z_add 增加一只僵尸在游戏中z_spawn bossname 增加一只Boss怪物,bossname分别是:tank, boomer, smoker。

PureWick 女性外用腹腔引流器说明书

PureWick 女性外用腹腔引流器说明书

Instructions for UseAttach to hospital wall suction or D ry D oc TM Vacuum Tuck betweenAlign with Place gauzeSetup:1. Connect the canister to wall suctionand set to a minimum of 40mmHgcontinuous suction. Always use theminimum amount of suction necessary.If using the D ry D oc TM Vacuum Station,connect the canister to the unit andturn the unit on. Please consult theD ry D oc TM Vacuum Station User Guidefor further information.2. Using standard suction tubing, connectthe P ure W ick TM Female ExternalCatheter to the collection canister.Peri-care and Placement:3. Perform perineal care and assessskin integrity (document per hospitalprotocol). Separate legs, gluteusmuscles, and labia. Palpate pubicbone as anatomical marker.4. With soft gauze side facing patient,align distal end of the P ure W ick TMFemale External Catheter at glutealcleft. Gently tuck soft gauze sidebetween separated gluteus and labia.Ensure that the top of the gauze isaligned with the pubic bone. Slowlyplace legs back together oncethe P ure W ick TM Female ExternalCatheter is positioned.Note: Patient can be positioned on back,side lying, frog legged, or lying on backwith knees bent and thighs apart (lithotomyposition) prior to device placement.Maintenance:6. Replace the P ure W ick TM Female External Catheter at least every 8-12 hours or if soiled with feces or blood. Always assess skin for compromise and perform perineal care prior to placement of a new P ure W ick TM Female External Catheter.Removal:5. To remove the P ure W ick TM Female ExternalCatheter, fully separate the legs, gluteus,and labia. To avoid potential skin injury uponremoval, gently pull the P ure W ick TM Female External Catheter directly outward. Ensuresuction is maintained while removing theP ure W ick TM Female External Catheter. Afteruse, this product may be a potential biohazard.Dispose of in accordance with applicable local,state and federal laws and regulations.FEMALE EXTERNAL CATHETER P ure W ickIndication for use:The P ure W ick TM Female External Catheter is intended for non-invasive urine output management in female patients. Caution: This Product Contains Dry Natural Rubber Contraindications: • Patients with urinary retention Warnings:• Do not use the P ure W ick TM Female External Catheter with bedpan or any material that does not allow for sufficient airflow.• To avoid potential skin injury, never push or pull the P ure W ick TM Female External Catheter against the skin duringplacement or removal.• Never insert the P ure W ick TM Female External Catheter into vagina, anal canal, or other body cavities.• Discontinue use if an allergic reaction occurs.• After use, this product may be a potential biohazard. Dispose of in accordance with applicable local, state and federal laws and regulations.Precautions: • Not recommended for patients who are: ▫ Agitated, combative, or uncooperative and might remove the P ure W ick TM Female External Catheter ▫ Having frequent episodes of bowel incontinence without a fecal management system in place ▫ Experiencing skin irritation or breakdown at the site ▫ Experiencing moderate/heavy menstruation and cannot use a tampon • Do not use barrier cream on the perineum when using the P ure W ick TM Female External Catheter. Barrier cream may impede suction. • Not recommended for use on patients with a known latex allergy. • Proceed with caution in patients who have undergone recent surgery of the external urogenital tract. • Always assess skin for compromise and perform perineal care prior to placement of a new P ure W ick TM Female External Catheter. • Maintain suction until the P ure W ick TM Female External Catheter is fully removed from the patient to avoid urine backflow.Recommendations:• Perform each step with clean technique. In the home setting, wash hands thoroughly before device placement.• Prior to connecting the P ure W ick TM Female External Catheter to hospital wall suction tubing, verify suction function by covering theopen end of the suction tubing with one hand and observing the pressure dial. If the pressure does not increase when the line iscovered, verify that the tubing is secured, connected, and not kinked.• Ensure the P ure W ick TM Female External Catheter remains in the correct position after turning the patient. Remove the P ure W ick TMFemale External Catheter prior to ambulation.• Properly placing the P ure W ick TM Female External Catheter snugly between the labia and gluteus holds the P ure W ick TM Female External Catheter in place for most patients. Mesh underwear may be useful for securing the P ure W ick TM Female ExternalCatheter for some patients.• Assess device placement and patient’s skin at least every 2 hours.• Replace the P ure W ick TM Female External Catheter every 8-12 hours or when soiled with feces or blood. • Change suction tubing per hospital protocol or at least every thirty (30) days.EXTERNAL USE ONLY FEMALE EXTERNAL CATHETER P ure W ick。

PacBio SEQUELII 准备 HiFi SMRTbell 库说明书

PacBio SEQUELII 准备 HiFi SMRTbell 库说明书

Procedure & Checklist – Preparing HiFi SMRTbell®Libraries using the SMRTbell Express Template Prep Kit 2.0This procedure describes the construction of HiFi SMRTbell libraries for de novo assembly and variant detection applications using the SMRTbell Express Template Prep Kit 2.0 and recommended HiFi sequencing conditions using PacBio’s new Sequel® II Binding Kit 2.2. A minimum input amount of 5 µg of high-molecular weight genomic DNA is recommended for generating HiFi library yields sufficient for running multiple SMRT®Cells on the Sequel II or Sequel IIe System (Sequel II Systems). Note that final HiFi library construction yields will be dependent on the specific size-selection method employed.We recommend fragmenting the gDNA so that the target size distribution mode is between 15 kb - 18 kb. To reduce the presence of fragments >30 kb, PacBio recommends a 2-cycle shearing method on the Megaruptor 3 system. Generally, a narrower fragment size distribution results in more uniform and higher-quality HiFi data. Details regarding DNA shearing conditions (e.g., buffers and DNA sample concentration) are described in the “DNA Requirements for Shearing” section.RequiredEquipment Vendor Throughput Run TimeFemto Pulse AgilentTechnologies Process up to 11 samples per runBatch process up to 88 samples 85 minsMegaruptor 3 Diagenode Shear up to 8 samples at a time40 mins(for 1 cycle of shearing)PippinHT Sage Science Maximum of 20 samples per instrument run 2 hrsBluePippin Sage Science Maximum of 4 samples per instrument run 4.5 hrsSageELF Sage Science Maximum of 2 samples per instrument run 4.5 hrs Table 1: Recommended equipment for HiFi SMRTbell library construction for de novo assembly and variant detection applications.Required MaterialsDNA SizingFemto Pulse Agilent Technologies, Inc. P-0003-0817DNA QuantitationQubit™ Fluorometer ThermoFisher Scientific Q33238Qubit 1X dsDNA HS Assay Kit ThermoFisher Scientific Q33230DNA ShearingMegaruptor 3 System Diagenode B06010003Megaruptor 3 Shearing Kit Diagenode E07010003SMRTbell Library PreparationSMRTbell® Express Template Prep Kit 2.0 PacBio 100-938-900AMPure® PB Beads PacBio 100-265-900SMRTbell® Enzyme Clean Up Kit 2.0 (New*) PacBio 101-932-600Sequencing Primer v5 (New*) PacBio 102-067-400100% Ethanol, Molecular Biology Grade Any MLSWide Orifice Tips (Tips LTS W-O 200UL Fltr RT-L200WFLR) Rainin 30389241Lo-Bind 0.2 mL tube strips USA Scientific, TempAssure1402-4708Multi-channel Pipette Rainin, 17013810Magnetic separation rack V&P Scientific, Inc, VP 772F4-1Thermal Cycler that is 100 µL and 8-tube strip compatible Any MLSSize-selection (One of the following systems)PippinHT System Sage Science HTP00010.75% Agarose Gel Cassettes, Marker 75E Sage Science HPE7510BluePippin System Sage Science BLU00010.75% Agarose Cassettes, Marker S1 Sage Sciences BLF7510SageELF System Sage Science ELF00010.75% Agarose Cassettes Sage Science ELD7510SequencingSequel® II Binding Kit 2.2 (New*)PacBio 101-894-200Sequel® II Sequencing Kit 2.0 PacBio 101-820-200SMRT® Cell 8M Tray PacBio 101-389-001* To obtain a copy of the previous version of this Procedure & Checklist that specifies use of SMRTbell Enzyme Clean Up Kit (PN 101-746-400) and Sequencing Primer v2 (PN 101-847-900), contact ****************.HiFi Library Construction WorkflowPacBio recommends that gDNA samples be resuspended in an appropriate buffer (e.g., Qiagen Elution Buffer) before proceeding with DNA shearing.Figure 1: Workflow for preparing HiFi libraries using the SMRTbell Express Template Prep Kit 2.0.Reagent HandlingSeveral reagents in the SMRTbell Express Template Prep Kit 2.0 (shown in Table 2 below) are sensitive to temperature and vortexing. We recommend to:•Never leave reagents at room temperature.•Always work on ice when preparing master mixes.•Finger-tap followed by a quick spin prior to use.Reagent Where UsedDNA Prep Additive Remove single-strand overhangsDNA Prep Enzyme Remove single-strand overhangs DNA Damage Repair Mix v2 DNA Damage RepairEnd Prep Mix End-Repair/A-tailingOverhang Adapter v3 LigationLigation Mix LigationLigation Additive LigationLigation Enhancer LigationSMRTbell Enzyme Clean Up Mix Nuclease TreatmentSMRTbell Enzyme Cleanup Buffer 2.0 Nuclease TreatmentTable 2: Temperature sensitive reagentsGenomic DNA (gDNA) Quality EvaluationThis procedure requires high-quality, high-molecular weight input gDNA with a majority of the DNA fragments >50 kb as determined by pulsed-field gel or capillary electrophoresis. Any of the three commercially available systems listed in Table 4 below may be used to evaluate gDNA quality, but the Femto Pulse system is highly recommended for high-throughput library construction due to its ability to rapidly process multiple samples in a single run using very low amounts (<1 ng) of DNA per sample. Links to recommended procedures for each system are also provided in the table. Examples of gDNA quality assessment using Bio-Rad’s CHEF Mapper (2A) and Agilent Technologies’ Femto Pulse (2B) are shown in Figure 2. Lanes A3 and B1 correspond to high-quality gDNA samples that are suitable for HiFi library construction using this procedure. Lanes A4 and B2 show degraded gDNA samples that not suitable for use in this procedure.Method ProcedureFemto Pulse Agilent Technologies, Inc.Bio-Rad CHEF Mapper XA Pulsed Field Electrophoresis System Procedure & Checklist - Using the BIO-RAD® CHEF Mapper® XA Pulsed Field Electrophoresis SystemSage Science Pippin Pulse Procedure & Checklist - Using the Sage Science PippinPulse Electrophoresis Power Supply SystemTable 3. gDNA Quality Evaluation Methods and Procedures.Figure 2: Evaluation of high-molecular weight gDNA quality using two DNA sizing analysis systems. A) Bio-Rad CHEF Mapper and B) Agilent Technologies’ Femto Pulse.165.510 kb 50 kb 42 kb33 kb 21 kb 17.7 kb 1.3 kb1 bpLane 1: 8 kb - 48 kb Ladder (Bio-Rad) Lane 2: 5 kb ladder (Bio-Rad) Lane 3: HMW gDNA Lane 4: Degraded gDNALane 1: HMW gDNALane 2: Degraded gDNA Lane 3: 165 kb ladder48 kb-20 kb-80 kb----------10 kb-14322 1 3ABDNA Requirements for ShearingBefore shearing, ensure that the genomic DNA is in an appropriate buffer (e.g.,Qiagen Elution Buffer, 10 mM Tris-Cl, pH 8.5 or PacBio EB buffer). If you are unsure of the buffer composition or if the gDNA is not in Elution Buffer, perform a 1X AMPure PB bead purification followed by elution with Elution Buffer or an equivalent low salt buffer (i.e., 10 mM Tris-Cl, pH 8.5- 9.0).PacBio highly recommends Diagenode’s Megaruptor 3 system for shearing gDNA. The Megaruptor 3 system allows up to 8 gDNA samples to be processed simultaneously with a consistent fragment size distribution across multiple hydropore-syringes. Furthermore, the Megaruptor 3 system generates a narrower size distribution than the g-TUBE device (Covaris).Shearing Using Diagenode’s Megaruptor 3 SystemTo maximize HiFi yield per SMRT Cell, PacBio recommends fragmenting the gDNA to a size distribution mode between 15 kb – 18 kb for human whole genome sequencing. Libraries with a size distribution mode larger than 20 kb are not recommended for HiFi sequencing. Recommended library insert size distributions to use for different WGS applications are summarized in Table 4 below.Application Recommended Library Insert SizeHuman Variant Detection 15 – 18 kbHuman de Novo15 – 18 kbPlant/Animal de Novo15 – 20 kbTable 4: Library size recommendations for Human variant detection and de novo assembly.To shear gDNA on the Megaruptor 3 system, use a two-cycle shear method, which requires running a second round of shearing immediately following the first fragmentation step in the same hydropore-syringe. The recommended concentration is 83.3 ng/µL (5 µg of input DNA in 60 µL Elution Buffer).The DNA shearing guidelines below have been tested by PacBio on the Megaruptor 3 system only. The response of individual gDNA samples to the shearing recommendations described below may differ; therefore, performing a small-scale test shear is highly recommended, including the Megaruptor 3 system.For the Megaruptor and Megaruptor 2 systems, shearing optimization is necessary before proceeding with this Procedure & Checklist. The shearing procedure described in the “Shearing Using Diagenode’s Megaruptor 3 system” section below is not compatible with the Megaruptor or Megaruptor 2 systems. For Megaruptor and Megaruptor 2 systems, follow Diagenode’s DNA shearing recommendations described in their manual. For additional guidance, contact Technical Support or your local FAS.The g-TUBE device generates a broader DNA fragment size-distribution compared to the Megaruptor 3 system. Note that HiFi read quality and overall HiFi data yield may be reduced due to the residual presence of large DNA fragments generated by g-TUBEs. For additional guidance, contact Technical Support or your local FAS.Figure 3: Examples of human genomic DNA samples sheared to a target 15 kb - 18 kb size distribution mode using a 2-cycle shear method on the Megaruptor 3 system.Prepare SMRTbell LibrariesAlways work on ice throughout the library construction process. To process multiple samples at a time, the following equipment are required:• Lo-Bind tube strips• Multi-channel pipette• Wide-bore tips• Magnetic rack compatible with tube strips• Thermocycler compatible with tube stripsRemove Single-Strand OverhangsThe sample volume recovered from the Megaruptor 3 system after shearing is used directly in the single-strand overhang digestion step. Before proceeding, ensure that the sheared DNA is in Elution Buffer or an equivalent low salt buffer (i.e., 10 mM Tris-Cl, pH 8.5- 9.0). In this step, DNA Prep Additive is diluted first followed by digestion. Scale up the reaction volumes for digestion if working with multiple samples.1. Prepare the DNA Prep Additive. The DNA Prep Additive is diluted with Enzyme Dilution Buffer toa total volume of 5 µL. This amount is sufficient for processing 1 to 4 samples. The volume maynot be sufficient for 5 samples due to pipetting errors. We recommend scaling up the dilutionvolume based on the number of samples to be processed (example: prepare 2X volume for 8samples and 4X volume for 16 samples).Note: The diluted DNA Prep Additive should be used immediately and should not be stored.2. Prepare the digestion by following the reaction table below. For multiple samples, prepare amaster mix, followed by addition of 10.0μL master mix to each sheared DNA sample.3. Add 10.0 µL of the above master mix to the tube-strips containing 45.0 µL - 53.0 µL of shearedDNA. The total volume in this step is 55.0 µL - 63.0 µL.4. Using a multi-channel pipette, mix the reaction wells by pipetting up and down 10 times with wide-orifice pipette tips.5. Spin down the contents of the tube strips with a quick spin in a microfuge.6. Incubate at 37°C for 15 minutes, then return the reaction to 4°C.7. Proceed to the next step.Repair DNA DamageTo each Reaction Mix 1, add 2.0 µL of DNA Damage Repair Mix v2.1. Mix the reaction well by pipetting up and down 10 times with wide-orifice pipette tips.2. Spin down the contents of the tube strips with a quick spin in a microfuge.3. Incubate at 37°C for 30 minutes, then return the reaction to 4°C.4. Proceed to the next step.End-Repair/A-tailingTo each Reaction Mix 2, add 3.0 µL of End Prep Mix.1. Mix the reaction well by pipetting up and down 10 times with wide-orifice pipette tips.2. Spin down the contents of the tube strips with a quick spin in a microfuge.3. Incubate at 20°C for 10 minutes.4. Incubate at 65°C for 30 minutes, then return the reaction to 4°C.5. Proceed to the next step.Adapter LigationIn this step, 5.0 µL of Overhang Adapter is added to each Reaction Mix 3 (from the previous step). Then, 32.0 µL of the ligase master mix is added to each Reaction Mix 3/Adapter Mix for incubation. Always work on ice. 1. To each Reaction Mix 3, add 5.0 µL of Overhang Adapter.2. Mix the reaction well by pipetting up and down 10 times with wide-orifice pipette tips. Leave the tube strips on ice.3. Prepare a Master Mix containing Ligation Enhancer, Ligation Additive and Ligation Mix using the table4. Mix the reaction well by pipetting up and down 10 times with wide-orifice pipette tips. It is important to mixwell.5. To the Reaction Mix 3/Adapter Mix, add 32.0 µL of the Ligase Master Mix. The total volume in this step is97.0 µL- 105.0 µL.6. Mix the reaction well by pipetting up and down 10 times with wide-orifice pipette tips. It is important to mixwell.7. Incubate at 20°C for 1 hour. Optional: The Ligation reaction may also be left at 20°C overnight.8. Proceed to the next step.Purify SMRTbell Library Using 1.0X AMPure® PB BeadsPage 11 PN 101-853-100 Version 05(August 2021)Nuclease Treatment of SMRTbell LibraryTo each library sample, add the nuclease mix to remove damaged SMRTbell templates.1. Prepare a Master Mix of the Enzyme Cleanup Mix and Buffer.2. Mix the reaction well by pipetting up and down 10 times with wide-orifice pipette tips. It is important to mixwell.3. Spin down the contents of the tube strips with a quick spin in a microfuge.4. To each 15.0μL of sample, add 55.0 μL of Nuclease Master Mix. The total reaction volume at this step is70.0 µL.5. Mix the reaction well by pipetting up and down 10 times with wide-orifice pipette tips. It is important to mixwell.6. Incubate at 37°C for 30 mins and store on ice immediately.7. Spin down the contents of tube strips with a quick spin in a microfuge.8. Proceed directly to the AMPure PB bead purification step below immediately. Do not store samples at thisstage. Do not let samples sit for long periods of time. Always work on ice.Page 12 PN 101-853-100 Version 05(August 2021)Purify SMRTbell Library Using 1.0X AMPure® PB BeadsSize Selection of SMRTbell LibrariesFor high-throughput whole genome sequencing applications, PacBio highly recommends the PippinHT system (Sage Science) for size-selection of SMRTbell libraries for HiFi sequencing. Typical recovery yields are 35% - 50% and are highly dependent on the size distribution of the starting SMRTbell library.Size Selection Using the PippinHT SystemVerify that your PippinHT system software is up to date and follow the procedure below to remove SMRTbellSize Selection Using the BluePippin SystemSage Science’s BluePippin system may also be used for size-selection of HiFi SMRTbell libraries. Verify that your BluePippin system software is up to date and follow the procedure below to remove SMRTbell templates <10 kb using the BluePippin system. Typical recovery yields are highly dependent on the size distribution of the starting SMRTbell library. For the latest BluePippin system User Manual and guidance on size-selection protocols, contact Sage Science ().Size Selection Using the SageELF SystemSage Science’s SageELF system may also be used to fractionate SMRTbell libraries for HiFi whole genome sequencing applications. Verify that your SageELF system software is up to date and follow the size selection procedure below. For the latest SageELF User Manual and guidance on size-selection protocols, contact Sage Science ().6Set up the run Protocol:– In the “Protocol Editor” tab, click on the “New Protocol” button.– Select the “0.75% 1-18kb v2” in the cassette definition menu.– Select “size-based” for separation mode.– Enter 3450 in the “Target Value” field and move the bar slider to selectwell #12.– Save as new protocol.– On the Main screen, clear previous run data, select cassette description,cassette definition and protocol, enter sample ID(s).– Select in the Nest Selector the cartridge that will be run.7Start the run.8 Once the run is complete, (approximately 4.5 hours), collect 30 μL of the respectivefractions from the elution wells. Fractions of interest are typically ~11 kb, ~13 kb,~15 kb, ~17 kb.9 Check the sizes of all 12 fractions by loading on a Femto Pulse. To determine theaverage library size, perform a smear analysis by selecting the region of interestby defining the start and end points of the fractions.10 Pool together fractions that have an average library size 10 – 20 kb.11 Proceed to the AMPure PB Bead purification step.Purify Size-Selected HiFi Library Fractions with 1.0X AMPure ® PB BeadsSequencing PreparationSee Quick Reference Card - Loading and Pre-Extension Recommendations for Sequel II/IIe Systems .For Research Use Only. Not for use in diagnostic procedures. © Copyright 2020 - 2021, Pacific Biosciences of California, Inc. All rights reserved. Information in thisdocument is subject to change without notice. Pacific Biosciences assumes no responsibility for any errors or omissions in this document. Certain notices, terms, conditions and/o r use restrictions may pertain to your use of Pacific Biosciences products and/or third p arty products. Please refer to the applicable PacificBiosciences Terms and Conditions of S a le and to the applicable license terms at /lice nses.html. Pacific Biosciences, the Pacific Biosciences logo, PacBio, S M RT, SMRTbell, Iso-Seq and Sequel are trademarks of Pacific Biosciences. Femto Pulse and Fragment Analyzer are trademarks of Agilent Technologies. All other trademarks are the sole property of their respective owners.Revision History (Description)Version Date Initial release.01 September 2019 Internal revision with no content change (not uploaded to website).02 December 2019 On page 1, changed “HiFi reads” to just “Reads”. On page 12, under Repair DNA Damage,corrected “remove single strand overhangs” to “repair DNA damage”. On page 13, corrected “remove single strand overhangs” to “adapter ligation”.03 January 2020 Updated for SMRTbell Enzyme Clean Up Kit 2.0 and Sequencing Primer v5.04 April 2021 Removed SMRT Link Sample Setup and Run Design tables. Added reference to QRC.05August 2021。

2015 中国格兰普rix竞技车跑道指南说明书

2015 中国格兰普rix竞技车跑道指南说明书

From The FIA Formula One Race Director To All Teams, All Officials Document 2Date09 April 2015Time 09:012015 C HINESE G RAND P RIXThe FIA Formula One Race DirectorTitle Event NotesDescription Event NotesEnclosed Event Notes 09-04-2015_V1.pdfCharlie Whiting2015C HINESE G RAND P RIXFrom The FIA Formula One Race Director Document 2To Formula One Team Managers Date9 April 2015Time09.00E VENT N OTES9A PRIL 20151) Issues arising from the Malaysian Grand Prix2) Changes to the circuit2.1The run-off area has been changed from gravel to asphalt in the pit entry.2.2The race control CCTV system has been upgraded to high definition cameras and screens. 2.3 A debris fence has been installed in the pit entry to protect marshals.3) Pit lane map3.1Safety Car lines.3.2The location of the pit entry and the pit exit.3.3Designated garage areas.3.4Safety Car position for first lap and rest of race.3.5Blue flag marshal.3.6Safety Car “arrow man”.4) Weighing and weighing platform4.1The weighing platform will be open between the following times, each visit should last nomore than 10 minutes unless no other team is waiting in the pit lane :a) From 09.00 Thursday until 14.30 on Saturday (between 13.00 and 14.30 each visit will berestricted to five minutes).b) From when the cars are returned to the teams after qualifying until 19.30 on Saturday.c) From 09.00 until 13.00 on Sunday.Any team found to be abusing the time limits set out above, which we will be enforced by FIA security personnel and our own CCTV, will not be permitted to use the weighbridge againduring the Event.4.2Please ensure your staff treat all equipment in the FIA garage as if it was their own, theyshould also leave the area clean and tidy and keep their numbers within the garage to aminimum. Again, in the event of any reported abuse the team concerned will not bepermitted to use the weighbridge again during the Event.5) Practice starts5.1Practice starts may only be carried out on the left (in the fast lane) at the pit exit before theend of the pit wall. Drivers must leave adequate room on their right for another driver to pass.5.2During the time that the pit exit is open for reconnaissance laps (13.30-13.45 on Sunday)practice starts should be carried out on the track after leaving the pit lane, but withoutcrossing the dotted white line referred to in 6.3 below. Drivers wishing to carry out a startshould stop on the right in order to allow other cars to pass on their left.During this time any driver passing a car which has stopped to carry out a practice start may cross the white line that is referred to in 6.1 below. Any driver crossing this line must move back to the right of it as quickly as possible.6) Lines, bollards and flags at the pit entry and pit exit6.1In accordance with Chapter 4 (Section 5) of Appendix L to the ISC drivers must keep to theright of the white line at the pit exit when leaving the pits.6.2For safety reasons drivers must keep to the right of the bollard at the pit entry.6.3The dotted white line across the pit exit is the track edge.6.4If there is a yellow flag waved on the driver’s right at the beginning of the pit entry it will bewarning of an incident around the corner of the pit entry. This flag is not intended for drivers staying on the track and competing a lap.7) Light panels7.1 The FIA light panels have been installed in the positions shown on the circuit map. Inaccordance with Appendix H to the ISC the light signals have the same meaning as flag signals.8) DRS8.1DRS will be globally disabled if panels 1, 16, 17, 19 or 20 are displaying yellow.8.2Detection will be automatically disabled if the light panels below are displaying yellow :Zone 1: Panels 13, 14 or 15.Zone 2: Panel 18.8.3If automatic detection is not working , and permission has been given by race control to usemanual detection, DRS should not be used in the relevant zone if panels 13, 14, 15 or 18 are displaying yellow.9) Drivers leaving their pit stop position in the pit lane9.1For safety reasons, no car should be driven from its pit stop position at any time unless :a) It has first been driven into the pit stop position having just entered the pit lane fromthe track, and ;b) It is then driven immediately back onto the track from the pit stop position.10) Fire extinguishers around the circuit10.1 Indicated by small fluorescent orange boards.11) Places to remove cars from the track11.1 Indicated by fluorescent orange panels on the walls or guardrails.12) Support races12.1Teams are asked to keep their barriers no more than five metres from the garages during thesupport race sessions and races.13) Post qualifying parc fermé13.1The cameras should be installed and operated in the same way as 2014.14) Removing cars from the grid14.1Two gates in the pit wall, beside grid positions 5 and 18.15) Car number boards for the starts15.1On the driver's right.16) Post race parc fermé16.1All cars must enter the pit lane and proceed directly to the weighing area.17) Any other business17.1DRS disabling and re-enabling during and after a VSC period.17.2The new track camera system and possible effects on the “follow car” system.Charlie WhitingFIA Formula One Race DirectorV e r s i o n 1 –9 A p r i l 2015PI T L A N E S T A R T S PI T L A N E E N D S SAF ET YCA RLINE2。

鸟巢导游词英文

鸟巢导游词英文

鸟巢导游词英文(经典版)编制人:__________________审核人:__________________审批人:__________________编制单位:__________________编制时间:____年____月____日序言下载提示:该文档是本店铺精心编制而成的,希望大家下载后,能够帮助大家解决实际问题。

文档下载后可定制修改,请根据实际需要进行调整和使用,谢谢!并且,本店铺为大家提供各种类型的经典范文,如工作报告、合同协议、心得体会、条据书信、规章制度、礼仪常识、自我介绍、教学资料、作文大全、其他范文等等,想了解不同范文格式和写法,敬请关注!Download tips: This document is carefully compiled by this editor. I hope that after you download it, it can help you solve practical problems. The document can be customized and modified after downloading, please adjust and use it according to actual needs, thank you!Moreover, our store provides various types of classic sample essays, such as work reports, contract agreements, personal experiences, normative letters, rules and regulations, etiquette knowledge, self introduction, teaching materials, complete essays, and other sample essays. If you want to learn about different sample formats and writing methods, please pay attention!鸟巢导游词英文鸟巢导游词英文范文(通用6篇)作为一名乐于为游客排忧解难的导游,通常需要准备好一份导游词,导游词的主要特点是口语化,此外还具有知识性、文学性、礼节性等特点。

世界各地踢足球英语作文

世界各地踢足球英语作文

Playing soccer is a beloved sport across the globe, enjoyed by millions of people in various countries. Heres a composition about the universal love for soccer:Soccer, known as football in many parts of the world, is more than just a game its a passion that unites people from different cultures, languages, and backgrounds. The sports simplicity and universality make it accessible to all, regardless of age or social status.The Origins and Spread of SoccerSoccer has ancient roots, with versions of the game being played as far back as the second and third centuries BC in places like China and Greece. However, the modern game of soccer as we know it today originated in England in the mid19th century. The sport quickly spread across Europe and then to other continents, becoming a truly international phenomenon.Soccer in Different CountriesIn Brazil, soccer is not just a sport its a way of life. The country has produced some of the worlds greatest players, such as Pelé and Ronaldo, and the passion for the game is evident in every corner of the nation. Brazilians take pride in their soccer skills and their national teams achievements.In Europe, soccer enjoys immense popularity, with leagues like the English Premier League, Spanish La Liga, and the German Bundesliga drawing millions of viewers. The sport has a deeprooted history in these countries, with clubs like FC Barcelona and Manchester United having global fan bases.In Africa, soccer is a symbol of hope and unity. Countries like Nigeria, Cameroon, and Egypt have strong soccer cultures, and the sport serves as a platform for many young athletes to showcase their talents on the world stage.The Impact of SoccerSoccer has the power to bring people together. It transcends borders and serves as a common language for fans and players alike. International tournaments like the FIFA World Cup and the UEFA European Championship are not just sporting events they are celebrations of global unity and diversity.Moreover, soccer has a significant impact on the economy and society. It creates jobs, fosters tourism, and contributes to the development of sports infrastructure. It also plays a role in promoting health and wellbeing, as it encourages physical activity and teamwork.ConclusionSoccers global appeal is a testament to the sports ability to connect people from all walks of life. It is a game that can be played anywhere, from the lush grass of a professional stadium to the dusty grounds of an urban neighborhood. As the world continues to embrace soccer, the sport will undoubtedly continue to inspire, entertain, and bring joy to millions.。

部族制皮联盟详细流程

部族制皮联盟详细流程

部族制皮联盟详细流程The tribal tanning alliance is a comprehensive process that involves various steps and parties working together to produce high-quality leather products. 部族制皮联盟是一个包含多个步骤和各方合作的综合过程,旨在生产高质量的皮革制品。

The first step in the tribal tanning alliance process is gathering raw materials, which includes selecting the right type of animal hides for tanning. 部族制皮联盟过程的第一步是收集原材料,包括选择适合鞣制的动物皮革。

Once the animal hides are obtained, they undergo a thorough cleaning process to remove any debris and dirt. 一旦获得动物皮革,它们将经过彻底的清洁过程,去除任何碎片和污垢。

Next, the cleaned hides are treated with chemical solutions to prepare them for the actual tanning process. 接下来,清洁过的皮革将经过化学溶液处理,为实际的鞣制过程做准备。

The tanning process itself involves soaking the hides in tanning solutions, which can be done using traditional or modern methods.鞣制过程本身涉及将皮革浸泡在鞣制溶液中,可以使用传统或现代方法进行。

速降场地国际标准

速降场地国际标准

速降场地国际标准As an international standard for ski resorts, the FIS (International Ski Federation) sets guidelines for speed skiing venues to ensure the safety of athletes. These regulations cover the design and construction of the course, as well as the maintenance and preparation of the snow surface. 作为滑雪胜地的国际标准,国际滑雪联合会(FIS)制定了速降滑雪场地的指导方针,以确保运动员的安全。

这些规定涵盖了赛道的设计和施工,以及雪地表面的维护和准备。

One of the key aspects of a speed skiing venue is the slope angle, which needs to conform to specific FIS guidelines. The slope must be steep enough to allow for high speeds, but also needs to be safe for athletes to navigate. Additionally, the course layout should include challenging sections that test the skill and agility of competitors. 速降滑雪场地的一个关键要素是坡度角度,必须符合特定的FIS指导方针。

坡度必须足够陡峭,以便实现高速度,但同时也必须对运动员安全。

此外,赛道布局应包括挑战性的部分,测试参赛选手的技能和敏捷度。

pinebridge stadium介绍英文 范文模板

pinebridge stadium介绍英文 范文模板

pinebridge stadium介绍英文范文模板Pinebridge Stadium: A Premier Sports DestinationLocated in the heart of downtown Pinebridge, the Pinebridge Stadium is a world-class sports destination that attracts visitors from around the globe. With state-of-the-art facilities and a welcoming atmosphere, the stadium is amust-visit for any sports fan.The stadium features a massive seating capacity of 80,000, making it one of the largest stadiums in the world. It has played host to many high-profile sporting events, including international soccer matches and grand slam tennis tournaments. The stadium's design is modern and sleek, with sleek lines and an open-air feel that gives every spectator a great view of the action.One of the standout features of Pinebridge Stadium is its cutting-edge technology. From advanced video boards to wireless internet connections throughout the facility, fans have access to all the latest gadgets and tech. Furthermore,with ample parking available nearby, visiting Pinebridge Stadium is a breeze.Aside from being a premier sports destination, Pinebridge Stadium also plays an important role in local communities. It sponsors numerous youth sports programs and provides educational opportunities through internships and job training programs. The stadium's commitment to community outreach helps make it an inclusive environment where everyone can feel welcome.Overall, Pinebridge Stadium sets itself apart as one of the top sporting destinations in the world. Its impressive facilities and innovative technologies appeal to both local residents and tourists from around the globe. Whetheryou're looking for thrilling sports action or just want to relax in a beautiful outdoor environment, Pinebridge Stadium is definitely worth checking out.。

10 Reasons To Visit Majorca, Spain 西班牙马略卡岛

10 Reasons To Visit Majorca, Spain 西班牙马略卡岛

10 Reasons To Visit Majorca, Spain 西班牙马略卡岛Your destination for mesmeric beauties, museums & art galleries, parks & theaters, shops & shopping malls, beaches & bars, pubs & clubs, restaurants & hotels, and lots of delights & joys, Majorca is a Spanish jewel in the Mediterranean Sea. Majorca is one of the most beautiful of the Balearic Islands. You certainly have over a half score of reasons to visit the captivating island.Palma de Mallorca, the capital and the largest city of the island, is one of the major reasons to visit the amazing island of Majorca. The city is known for historic sites, restaurants, and vibrant nightlife. Some of the major attractions include Castell de Bellver, Pueblo Espa?ol, and the historical center of Palma famous for Catedral de Mallorca and Museo Catedralicio, Banys ?rabs, and Museo de Arte Espa?ol Contempor?neo.Port de Pollen?a is arresting town in the north of the island, in the foothills of the Serra de Tramuntana. The town is famous for its restaurants and lovely evenings. The chapel of El Calvari at the top of the hill and the Good Friday procession from the church to the church in town are the major attractions of the town.Alc?dia is fascinating town surrounded by 14th-century walls. It is perfect place for beaching and cycling. Situated on the outskirts of Alcudia, S'Alubufera National Park is the best bird watching location on the Island. Roman ruins and Old town are other attractions.Cap de Formentor is Majorca's most northerly point. Here, you can have nice views from the lighthouse. Home to numerous species of birds, the place is great attraction for bird lovers. Some of the common birds that you can watch here include falcons, rock doves, rare black vultures, grey, night and purple herons, reed warblers, and several types of plover. Valldemossa, the charming village in the Tramuntana mountains,is one of the must see sights of Majorca. The 14th-century monastery at Valldemossa, in the northwest, was home to Polish composer Chopin and the French feminist writer George Sand and her two children in 1838-39.The Monastery at Lluc is nestled in pretty countryside dotted with oak and cypress trees in the Serra de Tramuntana Mountains. The islanders undertake the pilgrimage to Lluc to see La Moreneta (The Little Dark One), a tiny and reputedly miraculous statue of the virgin on 12 September each year. Majorca's Caves are the major tourist attractions. The Dragon Caves (Coves del Drach in mallorquin) are the most visited caves. There are about 200 caves on the Island, but just a few are open to the public. Featuring underground lakes and stalactites, some caves have really become tourist traps.Majorca's Cuisine is wining, dinning, and enjoying. You can enjoy the ultra-traditional pa amb oli (bread and oil) comes in many forms, either simply served as local bread doused in oil or served with toppings such as ham, chorizo or local cheese. At tapas bars, you can enjoy frito mallorquin, a tasty mixture of fried vegetables, potatoes, meat and herbs. Some of the local dishes include ensa?mada, a pastry made with pork lard (sa?m), and sobrassada, sausage with lard and paprika.Majorca's beaches are the finest expressions of the placid beauty. Majorca offers you one of the quietest beaches. The beaches aren?? often busy, but you can enjoy a number of beach activities at beaches. The beaches are often kissed and fondled by the crystal-clear waters. Majorca is best known for peaceful beach vacations.Aquacity, located at the resort of S'Arsenal in Majorca, is the place where you can find several large pools, fast and exciting slides and playgrounds. You can really have nice time here with your kids. Marineland is the only amusement park in the Balearic Islands featuring performing dolphins and sea lions. The dolphins are always ??ye catchers??at the Marineland.Orson Johnson writes for Holiday Velvet, a website providing listings for bed and breakfasts, Majorca villas, hotels and Majorca holiday apartments。

中世纪2—全面战争城市英文名称

中世纪2—全面战争城市英文名称

中世纪2—全面战争城市英文名称{Abydos}阿拜多斯{Abydos_Region}阿拜多斯地区{Acre}阿克里{Acre_Province}阿克里地区{Adana}阿达纳{Adana_Province}阿达纳地区{Al_Basrah}巴士拉{Al_Suways}苏伊士{Alanya}阿兰亚{Alanya_Region}阿兰亚地区{Aleppo}阿勒颇{Aleppo_Province}阿勒颇地区{Alexandria}亚历山大利亚{Alexandria_Province}亚历山大利亚地区{Alia}阿利耶{Alia_Region}阿利耶地区{Amorium}阿摩利阿姆{Amorium_Region}阿摩利阿姆地区{Ankara}安卡拉{Ankara_Region}安卡拉地区{Antioch}安条克{Antioch_Province}安条克地区{Arab_Camp_2}贝尼海尔拉营地{Arab_Camp_3}贝尼哈立德营地{Arab_Camp_6}贝尼苏雷姆营地{Arsuf}阿苏夫{Arsuf_Region}阿苏夫地区{Ascalon}阿什凯隆{Ascalon_Region}阿什凯隆地区{Attaleia}阿塔雷亚{Attaleia_Region}阿塔雷亚地区{Baalbek_Region}巴勒贝克{Ballbek}巴勒贝克{Baghdad}巴格达{Baghdad_Province}巴格达地区{Banyas}班亚斯{Banyas_Region}班亚斯地区{Basra_Region}巴士拉地区{Caesarea}凯撒里亚{Caesarea_Province}凯撒里亚地区{Cairo}开罗{Cairo_Province}开罗地区{Constantinople}君士坦丁堡{Constantinople_Province}君士坦丁堡地区{Corum}乔鲁姆{Corum_Region}乔鲁姆地区{Damascus}大马士革{Damascus_Province}大马士革地区{Diyabakir}迪亚巴克{Diyabakir_Region}迪亚巴克地区{Doryleum}多利留姆{Doryleum_Region}多利留姆地区{Dumyat}杜姆亚特{Dumyat_Region}杜姆亚特地区{Edessa}埃德萨{Edessa_Province}埃德萨地区{Heraclea}赫拉克利亚{Heraclea_Region}赫拉克利亚地区{Homs}霍姆斯{Homs_Region}霍姆斯地区{Iconium}伊康{Iconium_Province}伊康地区{Iraklion}伊拉克吕翁{Isle_of_Crete}克里特岛{Isle_of_Rhodes}罗德岛{Jedda}苏莱曼东北营地{Jedda_Province}贝尼苏莱曼部落{Jerusalem}耶路撒冷{Jerusalem_Province}耶路撒冷地区{Kerak}卡拉克{Kerak_Region}卡拉克地区{Kermanshah}克尔曼沙阿{Kermanshah_Region}克尔曼沙阿地区{Kirkuk}基尔库克{Kirkuk_Region}基尔库克地区{Kirsehir}克尔谢希尔{Kirsehir_Region}克尔谢希尔地区{Krak_de_Chavliers_Region}骑士堡地区{Krak_de_Chevaliers}骑士堡{Kufa}库法{Kufa_Region}库法地区{Laodicea_Region}莱迪西亚地区{Laodiciea}莱迪西亚{Limassol}利马索尔{Malatya}马拉蒂亚{Malatya_Region}马拉蒂亚地区{Mosul}摩苏尔{Nicaea}尼西亚{Nicaea_Province}尼西亚地区{Nicosia}尼科西亚{Northern_Cyprus}尼科西亚地区{Palaeokastron}帕拉伊奥卡斯通{Palaeokastron_Region}帕拉伊奥卡斯通地区{Qarisiya}克瑞西亚{Qarisiya_Region}克瑞西亚地区{Raqqa}拉卡{Raqqa_Region}拉卡地区{Rhodes}罗德{Sebastea}塞巴斯蒂亚{Sebastea_Region}塞巴斯蒂亚地区{Sinope}锡诺普{Sinope_Region}锡诺普地区{Siwa}锡瓦{Siwa_Region}锡瓦地区{Smyrna}士麦那{Smyrna_Province}士麦那{Southern_Cyprus}利马索尔{Suways_Region}苏伊士地区{Tabriz}大不里士{Tabriz_Region}大不里士地区{Takrit}塔克利特{Takrit_Region}塔克利特地区{Tanta}坦塔{Tanta_Region}坦塔地区{Tbilisi}第比利斯{Tbilisi_Province}第比利斯地区{Theodoiopolis_Region}塞奥多西奥波利斯地区{Theodosiopolis}塞奥多西奥波利斯{Trebizond}特拉布宗{Trebizond_Province}特拉布宗地区{Tribelands2}贝尼海尔拉部落{Tribelands3}贝尼哈立德部落{Tribelands6}贝尼苏雷姆部落{Tripoli}的黎波里{Tripoli_Province}的黎波里地区{Tyre}提尔{Tyre_Region}提尔地区{Van}凡城{Van_Region}凡城地区{Yerevan}埃里温条顿地区与城市{Aarhus}奥尔胡斯{Abo}阿博{Agder}阿格德{Agder_Region}阿格德地区{Akershus}阿克什胡斯{Arensburg}阿伦斯伯格{Arensburg_Region}阿伦斯伯格地区{Baranavichy}巴拉诺维奇{Baranavichy_Region}巴拉诺维奇地区{Bialystok}比亚韦斯托克{Bialystok_Region}比亚韦斯托克地区{Bohemia}波希米亚{Central_Sweden}瑞典中部{Chernigov}切尔尼戈夫{Chernigov_Region}切尔尼戈夫地区{Chornobyl}切尔诺贝利{Chornobyl_Region}切尔诺贝利地区{Corland}克兰德{Cracow}克拉科夫{Czestochowa}琴斯托霍瓦{Czestochowa_Region}琴斯托霍瓦地区{Danzig}但泽{Denmark_Region}丹麦地区{Dokshitsy}多克西泽{Dokshitsy_Region}多克西泽地区{Dunaburg}多瑙堡{Finland_Region}芬兰地区{Gorlitz}格尔利茨{Gorlitz_Region}格尔利茨地区{Goteborg}哥德堡{Gotland}哥特兰{Halmstad}哈尔姆斯塔德{Halmstad_Region}哈尔姆斯塔德地区{Hamburg}汉堡{Hannover}汉诺威{Hannover_Region}汉诺威地区{Hassleholm}海斯勒霍尔姆{Hassleholm_Region}海斯勒霍尔姆地区{Hof}霍夫{Hof_Region}霍夫地区{Homyel}戈梅利{Homyel_Region}戈梅利地区{Hrodna}格罗德诺{Hrodna_Region}格罗德诺地区{Jazdow}贾兹都{Kalmar}卡尔马{Kalmar_Region}卡尔马地区{Kaunus}考纳斯{Kaunus_Region}考纳斯地区{Kholm}霍尔姆{Kholm_Region}霍尔姆地区{Kiev}基辅{Kiev_Region}基辅地区{Klodzko}克沃兹科{Klodzko_Region}克沃兹科地区{Konigsberg_Region}柯尼希堡地区{Konisberg}柯尼希堡{Koszalin}科沙林{Koszalin_Region}科沙林地区{L_vov}利沃夫{L_vov_Region}利沃夫地区{Lida}利达{Lida_Region}利达地区{Lithuania}立陶宛{Liuboml}琉博莫{Liuboml_Region}琉博莫地区{Livonia}立窝尼亚{Magdeburg}马格德堡{Magdeburg_Region}马格德堡地区{Marienburg}马林堡{Marienburg_Region}马林堡地区{Masovia}马素比亚{Mglin}姆格林{Mglin_Region}姆格林地区{Minsk}明斯克{Minsk_Region}明斯克地区{Mstislavl}姆斯季斯拉夫尔{Mstislavl_Region}姆斯季斯拉夫尔地区{Narva}纳尔瓦{Narva_Region}纳尔瓦地区{Neustettin}新斯德丁{Neustettin_Region}新斯德丁地区{North_Pskov_Region}普斯科夫北部{Northern_Germany}德意志北部{Norway_Region}挪威地区{Novgorod}诺夫哥罗德{Novgorod_Region}诺夫哥罗德地区{Olysta}奥里斯塔{Olysta_Region}奥里斯塔地区{Palanga}帕兰加{Palanga_Region}帕兰加地区{Panevezys}帕涅韦日斯{Panevezys_Region}帕涅韦日斯地区{Parnu_Region}派尔努地区{Pernau}波尔瑙{Pinsk}平斯克{Pinsk_Region}平斯克地区{Plock}普沃茨克{Plock_Region}普沃茨克地区{Pomerania}波美拉尼亚{Potsdam}波茨坦{Potsdam_Region}波茨坦地区{Poznan}波兹南{Prague}布拉格{Przeworsk}普热沃斯克{Przeworsk_Region}普热沃斯克地区{Pskov}普斯科夫{Reval}雷瓦尔{Reval_Region}雷瓦尔地区{Riga}里加{Roskilde}罗斯基勒{Siauliai}希奥利艾{Siauliai_Region}希奥利艾地区{Skara}斯卡拉{Skara_Region}斯卡拉地区{Smolensk}斯摩棱斯克{Smolensk_Region}斯摩棱斯克地区{Southern_Poland}波兰南部{Southern_Sweden}瑞典南部{Stettin}斯德丁{Stralsund}施特拉尔松德{Stralsund_Region}施特拉尔松德地区{Thorn}索恩{Thorn_Region}索恩地区{Tingvalla}廷韦拉{Tingvalla_Region}廷韦拉地区{Tosno}托斯诺{Tosno_Region}托斯诺地区{Turov}图罗夫{Turov_Region}图罗夫地区{Uelzen}于尔岑{Uelzen_Region}于尔岑地区{Uppsala}乌普萨拉{Velikiye_Luki}大卢基{Velikiye_Luki_Region}大卢基地区{Vilnius}维尔纽斯{Visby}维斯比{Vitebsk}维捷布斯克{Vitebsk_Region}维捷布斯克地区{West_Poland}波兰西部{West_Pomerania}波美拉尼亚西部{Windau}温道{Windau_Region}温道地区{Zealand}西兰{Zhytomyr}日托米尔{Zhytomyr_Region}日托米尔地区{Zubstov}泽布斯托夫{Zubstov_Region}泽布斯托夫地区美洲地区与城市{Aguapalam}阿瓜帕拉姆{Aguapalam_r}阿瓜帕拉姆地区{Ahuacatlan}阿瓦卡特兰{Ahuacatlan_r}阿瓦卡特兰地区{Alibamu}阿里巴姆{Alibamu_Region}阿里巴姆地区{Althun_Ha}阿顿哈{Althun_Ha_r}阿顿哈地区{Apache_Camp}阿帕奇{Apache_r}阿帕奇地区{Apatzignan}阿帕钦甘{Apatzignan_r}阿帕钦甘地区{Caddo}卡多{Caddo_r}卡多地区{Calusa}卡卢萨{Calusa_r}卡卢萨地区{Canpech}坎佩克{Canpech_r}坎佩克地区{Chetumal}切图马尔{Chetumal_r}切图马尔地区{Chichen_Itza}奇琴伊察{Chichen_Itza_r}奇琴伊察地区{Chickasaw}奇克索{Chickasaw_Region}奇克索地区{Chihuahua}奇瓦瓦{Chihuahua_r}奇瓦瓦地区{Choctaw}乔克托{Choctaw_r}乔克托地区{Cholula}乔鲁拉{Cholula_r}乔鲁拉地区{Coahulia}科阿韦拉{Coahulia_r}科阿韦拉地区{Coatzalcoalcos}夸赛夸尔科斯{Coatzalcoalcos_r}夸赛夸尔科斯地区{Cochimi}科奇米{Cochimi_r}科奇米地区{Colotan}科洛坦{Colotan_r}科洛坦地区{Comanche}科曼奇{Comanche_r}科曼奇地区{Cuahtemoc}夸特莫克{Cuahtemoc_r}夸特莫克地区{Cuba}古巴{Culiacan}库利亚坎{Culiacan_r}库利亚坎地区{Danipaguache}塔尼切卡奇{Danipaguache_r}塔尼切卡奇地区{Havana}哈瓦纳{Huaxtepec}瓦斯特佩克{Huaxtepec_r}瓦斯特佩克地区{Miccosoukee}密克苏基{Miccosoukee_r}密克苏基地区{Mitla}米克特兰{Mitla_r}米克特兰地区{Navojoa}纳瓦霍{Navojoa_Region}纳瓦霍地区{Ocotlan}奥科特兰{Ocotlan_r}奥科特兰地区{Pitic}皮蒂科{Pitic_r}皮蒂科地区{Potochan}波顿昌{Potochan_r}波顿昌地区{Quezaltenango}希拉杰{Quezaltenango_r}希拉杰地区{Quirigua}基里瓜{Quirigua_r}基里瓜地区{Tecoman}特科曼{Tecoman_r}特科曼地区{Tegucigalpa}特拉科奇卡潘{Tegucigalpa_r}特拉科奇卡潘地区{Tehuacan}特瓦坎{Tehuacan_r}特瓦坎地区{Tehuantepec}特宽特佩克{Tehuantepec_r}特宽特佩克地区{Tenochtitlan}特诺奇蒂特兰{Tenochtitlan_r}特诺奇蒂特兰地区{Tequesta}特奇斯塔{Tequesta_Region}特奇斯塔地区{Tikal}蒂卡尔{Tikal_r}蒂卡尔地区{Tixta_r}蒂科斯特拉地区{Tixtla}蒂科斯特拉{Tlaxcala}特拉斯卡拉{Tlaxcala_r}特拉斯卡拉地区{Toluca}托洛坎{Toluca_r}托洛坎地区{Tongva}通瓦{Tongva_Region}通瓦地区{Trujillo}特鲁希略{Trujillo_r}特鲁希略地区{Tula}托兰{Tula_r}托兰地区{Tututepec}托托特佩克{Tututepec_r}托托特佩克地区{Tuxpan}托奇潘{Tuxpan_r}托奇潘地区{Tuxtla}托奇特兰{Tuxtla_r}托奇特兰地区{Uxmal}乌斯马尔{Uxmal_r}乌斯马尔地区{VeraCruz_r}韦拉克鲁斯地区{Vera_Cruz}韦拉克鲁斯{Xicalango}希拉兰科{Xicalango_r}希拉兰科地区{Yamacraws}亚马科洛{Yamacraws_Region}亚马科洛地区{Yaxchilan}雅克奇兰{Yaxchilan_r}雅克奇兰地区{Zacapu}萨卡普{Zacapu_r}萨卡普地区{Zacatecas}萨卡特卡斯{Zacatecas_r}萨卡特卡斯地区英伦地区与城市{Aberdeen}阿伯丁{Aberdeen_Region}阿伯丁郡{Argyle_and_Bute}阿盖尔-比特{Arran}阿伦{Arundel}阿伦德尔{Athenry}阿森莱{Caernarvon}卡纳封{Caithness_and_Sutherland_Regions}凯斯内斯-萨瑟兰郡{Canterbury}坎特伯雷{Cardiff}加的夫{Carlisle}卡莱尔{Castle_Town}卡斯尔顿{Cheshire_Region}柴郡{Chester}切斯特{Connaught_Region}康诺特郡{Cork}科克{Cornwall_Region}康沃尔郡{Cumberland_Region}坎伯兰郡{Deheubarth_Principality}德赫巴思公国{Derry}德里{Dorsetshire_Region}多塞特郡{Down_Region}唐郡{Downpatrick}唐帕特里克{Dublin}都柏林{Dumfries}邓弗里斯{Dumfries_Region}邓弗里斯郡{Dunstaffnage}邓斯代夫纳奇{East_Anglia}东盎格鲁{Edinburgh}爱丁堡{Glasgow}格拉斯哥{Gloucester}格洛斯特{Gwynedd_Principality}格温内斯公国{Hampshire_Region}汉普郡{Highlands_Region}高地地区{Inverlochly}因弗洛克莱{Inverness}因弗内斯{Isle_of_Arran}阿伦岛{Isle_of_Islay}艾莱岛{Isle_of_Islay_Region}艾莱岛地区{Isle_of_Man}曼岛{Isle_of_Mull}马尔岛{Isle_of_Skye_Region}斯凯岛地区{Kent_Region}肯特郡{Kirkwall}柯克沃尔{Lanark_Region}拉纳克郡{Lancashire}兰开夏郡{Lancaster}兰开斯特{Launceston}朗塞斯顿{Leinster_Region}伦斯特郡{Lewis}斯托诺韦{Lifford}利福德{Lincoln}林肯{Lincolnshire}林肯郡{London}伦敦{London_Region}伦敦地区{Lothian_Region}洛锡安地区{Meath_Region}米斯郡{Montgomery}蒙哥马利{Morgannwg_Principality}摩根维格公国{Mull}马尔{Munster_Region}芒斯特地区{Newcastle_Upon_Tyne}泰恩-纽卡斯尔{Northumberland}诺森伯兰{Norwich}诺里奇{Nottingham}诺丁汉{Nottinghamshire}诺丁汉郡{Orkney_Region}奥克尼郡{Outer_Hebrides}外赫布里底群岛{Oxford}牛津{Oxford_Region}牛津地区{Pembroke}彭布罗克{Perth}珀斯{Perth_Region}珀斯郡{Powys_Principality}波厄斯公国{Shaftsbury}沙夫茨伯里{Shrewsbury}什鲁斯伯里{Shropshire_Region}什罗普郡{Skye}斯凯{Stirling}斯特林{Stirling_Region}斯特林郡{Surry_and_Sussex_Regions}萨里-萨塞克斯郡{Thomond_Region}托蒙德郡{Tipperary}蒂珀雷里{Trim}特里姆{Tyrconnel_Region}泰孔内尔地区{Ulster_Province}阿尔斯特郡{West_Country}韦斯特郡{West_Highlands}西高地地区{Wick}威克{Winchester}温切斯特{York}约克{Yorkshire}约克郡。

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Marketing im GrundstudiumLehrstuhl Marketing Technische Universität Berlin Prof. Dr. V. Trommsdorff Übung zur Vorlesung Marketing im Grundstudium Klaus Heine www.marketing-trommsdorff.deKlaus Heine Marketing - Grundstudium1Das magische Dreieck ist der Bezugsrahmen für die MarketingzieleKunden(Kundenproblem) (Bedarf und Nachfrage)UmfeldUnternehmen(Stärken und Schwächen)Wettbewerber(Stärken und Schwächen)Ziele MarktForschungStrategien InstrumenteProdukt Kommunikation Preis DistributionKlaus Heine Marketing - Grundstudium2Agenda: Preispolitik • • • • Grundlagen und Begriffe Preispolitische Strategien Konditionenpolitik Verfahren der PreisfestlegungKlaus Heine Marketing - Grundstudium3Einführung Preispolitik• Sie sind Geschäftsführer einer Fluggesellschaft, die hauptsächlich innereuropäische Linienflüge anbietet. Ihre Flugzeuge haben nur eine Klasse, Ihr Motto lautet: 1 Preis für alle. Ihre Ticketpreise liegen deutlich unter denen der Konkurrenz. In letzter Zeit erhalten Sie verstärkt Post von Geschäftsreisenden, die mangelnde Flexibilität bei der Reservierung und mangelnden Bord-Service, wie z.B. das Fehlen warmer Mahlzeiten, monieren. Dennoch sind alle gewillt, weiterhin mit Ihrer Gesellschaft zu fliegen, v.a. wegen der günstigen Ticketpreise. Nach reiflicher Überlegung entscheiden Sie sich zur Einführung einer Comfort-Klasse in ihren Flugzeugen: Die Tickets sind ohne Vorreservierung gültig und jederzeit umtauschbar; während des Fluges servieren Sie eine warme Mahlzeit und einen Begrüßungssekt. Dafür sind die Tickets doppelt so teuer. Zu Ihrem Erstaunen geht das Konzept auf. Stünden Sie als Fluggast vor der Entscheidung – welchen Tarif würden Sie wählen und warum?•Klaus Heine Marketing - Grundstudium4Was ist ein „Preis“ ?Der Preis ist die Summe aller Werte, welche die Konsumenten für Vorteile eintauschen, die der Besitz bzw. die Nutzung des Produktes / die Inanspruchnahme der Dienstleistung bieten.Produkt/LeistungKundenwünsche und -bedürfnisseWahrgenommener Nutzen des Produktes/ der LeistungPreisZahlungsbereitschaftAndere MarketinginstrumenteQuelle: Simon 1992Finanzielle Situation des KundenKlaus Heine Marketing - Grundstudium5VBegriffsklärungen zur PreispolitikKontrahierungspolitikPreispolitikKonditionenpolitik• • • •Definition und Vergleich alternativer Preisforderungen gegenüber aktuellen und potenziellen Abnehmern. Durchsetzung dieser Preise im Rahmen des intern und extern beschränkten EntscheidungsspielraumsEntscheidungen über: • (Positiv-/Negativ-) Rabatte • Absatzkredite • Lieferungs- und ZahlungsbedingungenKlaus Heine Marketing - Grundstudium6VDie Preis-Absatz-Funktion (preisspezifische Marktreaktionsfunktion) bildet die Abhängigkeit des Absatzes vom Preis abAbsatzmengeIIrelativ elastischIPreis-Absatz-Funktionenxrelativ unelastischpPreisKlaus Heine Marketing - Grundstudium7VDie Preiselastizität der Nachfrage bestimmt, wie relative Absatzmengen auf relative Preisänderungen reagierenDefinition Preiselastizität (ε) der Nachfrage Verhältnis der prozentualen Veränderung der Nachfrage nach einem Produkt zu der sie auslösenden prozentualen Änderung des Preises.dx ε= x dp pDie Preiselastizität variiert zwischen 0 und -∞ Extremwerte: ε = 0 → vollkommen unelastischer Markt, der Markt reagiert nicht auf eine Preisänderung (Bsp.: lebenswichtige Medikamente) ε = -∞ → vollkommen elastischer Markt, der Markt reagiert auf eine Preiserhöhung mit völligem Abnahmestopp (Bsp.: starke Substitutionsgüter: Computer-Chips) ε = -1 → Absatzänderung ändert sich in gleichem Maße wie PreisänderungKlaus Heine Marketing - Grundstudium8VPreiselastizität relativ unelastische NachfrageZur VertiefungPreissteigerung von 20 Euro auf 25 Euro also um 25% Nachfrageänderung von 55 auf 50 Stück also um 9,1% X 55 50dx − 5 ε = x = 55 = − 0,36 dp 5 p 20P 20 259Klaus Heine Marketing - GrundstudiumVPreiselastizität relativ elastische NachfrageZur VertiefungPreissteigerung von 20 Euro auf 25 Euro also um 25% Nachfrageänderung von 80 auf 10 Stück also um 87,5% X 80dx − 70 ε = x = 80 = − 3,5 dp 5 p 20P1010 20Klaus Heine Marketing - Grundstudium25VBeispiele für Preiselastizitätenx xp Salz x x Bananenpp Zigaretten einer bestimmten Marke Zigaretten insgesamtpKlaus Heine Marketing - Grundstudium11Definition des Begriffs Luxusprodukt aus volkswirtschaftlicher PerspektiveWas ist Luxus?Notwendigkeit Luxusprodukt 1 = Gewöhnliches Luxus Ein Produkt ist mehr als notwendig oder üblich2Klaus Heine Marketing - Grundstudium12Preiselastizitäten der wichtigsten ProduktkategorienKlaus Heine Marketing - Grundstudium13Übungsaufgabe zu den realen Verläufen von Preis-Absatz-FunktionenFolgende Preis-Absatz-Funktionen sind in der Realität beobachtet worden. Erläutern und begründen Sie deren Funktionsverläufe anhand von Erkenntnissen der verhaltenswissenschaftlichen Preistheorie.x(p)x(p)ppKlaus Heine Marketing - Grundstudium14Gibt es in der Realität solche Preis-Absatz-Funktionen?PXKlaus Heine Marketing - Grundstudium15Bist Du ein Snob?Klaus Heine Marketing - Grundstudium16Kategorien von Motiven des LuxuskonsumsRational Motives(Investment/Rationalization)MotivesSocial Motives Group-Belonging (Bandwagon) Demonstration of Wealth (Veblenian) Demonstration of Status (Snob) (Demonstration of Personality)Source, According to Allérès, Luxe… Métiers et Management Atypiques, 2nd edition, 2005, p. 51.Personal Motives Self-Directed Pleasure (Hedonist) Other-Related Pleasure Self-Gift-Gifting (and Collecting) Congruity with Internal Self Quality Assurance (Perfectionist)Klaus Heine Marketing - Grundstudium17The Most Important Ingredients of a Luxury Brand: Emotions & ImageMarginCommunication, Know-how, Image Production costsQuelle: Lebas/Israel-Russo/De Gouyon, Stratégies de luxe. Jouy-en-Josas: Groupe HEC 1990, p. 48.DiorKlaus Heine Marketing - Grundstudium18Das zeigt, dass verschiedene Kundensegmente aufgrund ihrer Persönlichkeitsmerkmale verschiedene Preiseinstellungen besitzenPremium-Käufer• Kaufen grundsätzlich nur das Beste und TeuersteDemonstrativ-Käufer• Kaufen Marken / Produkte für das Image - ungeachtet des PreisesBescheiden-Käufer• Verfolgen mit ihren Käufen kein Imageziel "Understatement"Billig-Käufer• Kaufen grundsätzlich nur das BilligsteKlaus Heine Marketing - Grundstudium19Agenda: Preispolitik • • • • Grundlagen und Begriffe Preispolitische Strategien Konditionenpolitik Verfahren der PreisfestlegungKlaus Heine Marketing - Grundstudium20VPreispolitische ZieleUnternehmensbezogen Erhöhung von • Marktanteil • Umsatz • Absatz • Gewinn • Deckungsbeitrag • ROI Handelsbezogen • Erhöhung der Präsenz in den Handelskanälen • Verbesserung der Produktplatzierung • Werbliche Unterstützung durch den Handel • Sicherung eines einheitlichen Preisniveaus Kundenbezogen Beeinflussung der: • wahrgenommenen Qualität • Preiswahrnehmung und -erwartung • wahrgenommene Preisgünstigkeit und -würdigkeitPreispolitische Ziele Preispolitische ZielePreiswürdigkeit: Ist der Preis im Verhältnis zur Qualität des Produktes gerechtfertigt? Preisgünstigkeit: Ist der Preis im Verhältnis zu Konkurrenzprodukten gerechtfertigt?Klaus Heine Marketing - Grundstudium21VPreispolitische StrategienA: FestpreisstrategienB: Strategien der Preisdynamik Festlegung der preispolitischen StoßrichtungC: Strategien der PreisabfolgeD: Strategien der PreisdifferenzierungKlaus Heine Marketing - Grundstudium22VDie Festpreisstrategien legen jeweils ein bestimmtes Preisniveau festHochpreisstrategie Ein Preis im oberen Preisniveau wird mittel- bis langfristig festgesetzt. Mittelpreisstrategie Ein Preis im mittleren Preisniveau wird mittel- bis langfristig festgesetzt. Niedrigpreisstrategie Ein Preis im unteren Preisniveau wird mittel- bis langfristig festgesetzt.PreisZeitKlaus Heine Marketing - Grundstudium23PreispositionierungsstrategienKlaus Heine Marketing - Grundstudium24Diversification of Luxury BrandsMain LineGiorgio Armani Dolce & Gabbana Donna Karan Calvin Klein Christian Lacroix Ralph Lauren Moschino Gianni Versace Jean Paul Gaultier Valentino Emanuel Ungarno Mani D&G DKNY CK Bazar Polo Cheap & Chic Versus JPG Oliver Ungarno Solo Donna Instante Ralph DSecond BrandEmporioThird BrandQuelle: Esteve/Hieu-Dess, Etude d’une strategie du luxe, 2005.Klaus Heine Marketing - Grundstudium25VPreisdynamik-Strategien orientieren sich an UmfeldbedingungenSaisonale Preisstrategie Wird bei saisonabhängigen Produkten verwendet: Spargel etc. Pulsierende Preisstrategie Wird verwendet, um langfristig Preiserhöhungen oder -senkungen durchzusetzen: Elektronikmarkt etc. Flexible PreisstrategieZeitPreisPasst sich sehr schnell und flexibel (nachfrageabhängig) an: Regenschirme, Großmarkthallen etc.Klaus Heine Marketing - Grundstudium26VPreisabfolgestrategien legen die Preisentwicklung von vornherein festPenetration-Strategie Sie beginnt mit einem niedrigen Preis, der im Laufe des PLZ angehoben wird.PreisSkimming-Strategie Sie beginnt mit einem hohen Preis, der im Laufe des PLZ gesenkt wird.ZeitKlaus Heine Marketing - Grundstudium27Strategien der PreisabfolgeKlaus Heine Marketing - Grundstudium28VDie Penetration-Strategie kann zum Aufbau einer starken Marktposition genutzt werdenGründe für und Wirkung der Penetration-Strategie • • • • • Niedrige Stück-Deckungsbeiträge, aber schnelles Wachstum Aufbau einer starken Marktposition durch hohe Distribution Ausnutzung von Stückkostendegression und Erfahrungskurve Geringere Flopwahrscheinlichkeit Abschreckung potenzieller KonkurrentenKlaus Heine Marketing - Grundstudium29VDie Skimming-Strategie realisiert hohe kurzfristige GewinneGründe für und Wirkung der Skimming-Strategie Gewinnrealisierung in monopolistischer Situation Schnelle Amortisation des F&E-Aufwandes möglich Positive Wirkungen von Preissenkungen nutzen Zeitliche Preisdifferenzierung Prestige- und Qualitätsindikation Langsamer Aufbau von (Produktions-)Kapazitäten• • • • • •Klaus Heine Marketing - Grundstudium30Quelle: Iyer, G., Pricing Strategies, 2000Versioning: Segmentierung, Targeting & Kannibalisierung Beispiel: Preisstrategie von DellPersonal Users Segmentgröße Dell 350 MHz Dell 550 MHz 60 € 500 € 750 Business Users 40 € 750 € 2500Gesamtübersicht Produktangebot Optimales Targeting Business & Personal Business 350 MHz für Personal 550 MHz für Business Gesamtumsatz € 50.000 €100.000Nur Dell 350 MHz Nur Dell 550 MHz Beide 350 MHz & 550 MHz€120.000Quelle: Iyer, G., Pricing Strategies, 2000Klaus Heine Marketing - Grundstudium41Versioning: Segmentierung, Targeting & Kannibalisierung Beispiel: Preisstrategie von Dell – Zusammenfassung• Konsumenten sind heterogen in ihrer Preissensitivität und Kaufbereitschaft Preise sollten deshalb entsprechend der Preissensitivität der Konsumenten festgelegt werden: Damit sollte man sicherstellen, dass Menschen mit inelastischer Nachfrage mehr bezahlen und Menschen mit elastischer Nachfrage weniger Schlüsselproblem: Kannibalisierung Man muss sicherstellen, dass Preise die an ein Segment gerichtet werden nicht durch ein anderes Segment (zu dessen Vorteil) genutzt werden können Wie wurde das bei Dell erreicht?••Quelle: Iyer, G., Pricing Strategies, 2000Klaus Heine Marketing - Grundstudium42Versioning: Beispiel Intel – Beschädigung von ProduktenEine wahre Geschichte. Es war einmal… • Problem für Intel als es den 486 Chip einführte: Es gab zwei Typen von Frühadoptoren E und G (50% von jedem Typ) • E ist willens mehr zu zahlen und führt besonders viele mathematische Operationen aus • G ist preissensibler und führt weniger mathematische Operationen aus Preisoptionen € 1000 € 800 • Segment E nur E kauft E&G beide kaufen Umsatz € 1000 € 1600Welchen Preis sollte Intel dem 486 geben?Quelle: Iyer, G., Pricing Strategies, 2000Klaus Heine Marketing - Grundstudium43Versioning: Beispiel Intel – Beschädigung von Produkten• Intel sollte verschiedene Preise verlangen: • € 800 von G • € 1000 von E • Gesamtumsatz: €1800 Preisdifferenzierung bringt Intel € 200 mehr Umsatz! Aber es gibt ein Problem…• •Quelle: Iyer, G., Pricing Strategies, 2000Klaus Heine Marketing - Grundstudium44Versioning: Beispiel Intel – Beschädigung von Produkten• • Intel will G weniger berechnen als E für den gleichen 486 Chip. D.h. Intel muss E behindern das (billigere) Produkt zu kaufen, das für G bestimmt ist. Was muss Intel also tun? Intel beschädigt den Math Coprocessor für G! • Dabei entstehen Kosten (angenommen: € 10) • Aber Intel bekommt dadurch € 200 mehr von E Intel beschädigt den Math Coprocessor des DX Chip und produziert so den SX Chip (dabei entstehen Intel also zusätzliche Kosten) D.h. Intel verkauft ein beschädigtes Produkt, das Intel mehr in der Herstellung kostet, für einen niedrigeren Preis•Quelle: Iyer, G., Pricing Strategies, 2000Klaus Heine Marketing - Grundstudium45Versioning: Beispiel Intel – Zusammenfassung: Zwei Methoden zur Kontrolle von KannibalisierungRisiko Kannibalisierung: Wenn die Low-end-Version zu attraktiv ist, kann sie Kunden anziehen, die sonst auch ein Preispremium für eine High-end-Version gezahlt hätten Das kann verhindert werden durch:•Methode 1: Senkung des Preises für die High-end-Version um sicherzustellen, dass die High-value-Kunden die High-end-Version kaufen Methode 2: Beschädigung der Low-end-Version ausreichend um sie unattraktiv genug zu machen für das High-end-Segment•Quelle: Iyer, G., Pricing Strategies, 2000Klaus Heine Marketing - Grundstudium46Versioning: Zusammenfassung der BeispieleGestaltung des Versioning Punkt 1: Identifikation von Attributen/Features, die hoch geschätzt werden von Kundensegmenten mit hoher Preisbereitschaft – und die gleichzeitig wenig wichtig sind für andere Kundensegmente Punkt 2: Um so größer die Unterschiede zwischen den Kunden bzgl. ihrer Preisbereitschaft für das differenzierende Attribut/Feature, um so weiter ist die Produktlinie, d.h. um so mehr Versionen sollte es geben (Problem: Wie viele?) Punkt 3: Finden der besten Möglichkeit zur Reduzierung von KannibalisierungDies zeigt den Zusammenhang von Produktstrategie, Preisstrategie und KundensegmentierungQuelle: Iyer, G., Pricing Strategies, 2000Klaus Heine Marketing - Grundstudium47Agenda: Preispolitik • • • • Grundlagen und Begriffe Preispolitische Strategien Konditionenpolitik Verfahren der PreisfestlegungKlaus Heine Marketing - Grundstudium48VBegriffsklärungen zur PreispolitikKontrahierungspolitikPreispolitikKonditionenpolitik• • • •Definition und Vergleich alternativer Preisforderungen gegenüber aktuellen und potenziellen Abnehmern. Durchsetzung dieser Preise im Rahmen des intern und extern beschränkten EntscheidungsspielraumsEntscheidungen über: • (Positiv-/Negativ-) Rabatte • Absatzkredite • Lieferungs- und ZahlungsbedingungenKlaus Heine Marketing - Grundstudium49VDie Konditionenpolitik regelt sonstige preispolitische MaßnahmenRabatte Positivrabatte: • (Handels-)Stufenrabatte • Mengenrabatte • Treuerabatte Negativrabatte • Mindermengenzuschläge Zuschläge Zugaben• Geldzuwendungen • Sachzuwendungen • Dienstleistungen• In Abhängigkeit von bestimmten Zeiten (z.B. nachts) • Entgelt für Mehr- und Sonderleistungen (oft im Baugewerbe)Klaus Heine Marketing - Grundstudium50。

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