Non-equilibrium capillary electrophoresis of equil

专利名称：Non-equilibrium capillary electrophoresis of equilibrium mixtures (NECEEM) - based
methods for drug and diagnostic
development
发明人：Sergey Krylov,Svetlana Krylova,Maxim
Berezovski
申请号：US10610547
申请日：20030702
公开号：US20050003362A1
公开日：
20050106
专利内容由知识产权出版社提供
摘要：The invention discloses a Non-Equilibrium Capillary Electrophoresis of Equilibrium Mixtures (NECEEM) method and NECEEM-based practical applications. The NECEEM method is a homogeneous technique, which, in contrast to heterogeneous methods, does not require affixing molecules to a solid substrate. The method of the invention facilitates 3 practical applications. In the first application, the method allows the finding of kinetic and thermodynamic parameters of complex formation. It advantageously allows for revealing two parameters, the equilibrium dissociation constant, K, and the monomolecular rate constant of complex decay, k, in a single experiment. In the second practical application, the method of this invention provides an approach for quantitative affinity analysis of target molecules. It advantageously allows for the use of affinity probes with relatively high values of k. In the third practical application, the method of this invention presents a new and powerful approach to select target-binding molecules (ligands) from complex mixtures. Unique capabilities of the
method in its third application include but not limited to: (a) the selection of ligands with pre-determined ranges of kinetic and thermodynamic parameters of target-ligand interactions, (b) the selection of ligands present in minute amounts in complex mixtures of biological or synthetic compounds such as combinatorial libraries of oligonucleotides, and (c) the selection of ligands for targets available in very low amounts. In particular, the method of this invention provides a novel approach for the selection of oligonucleotide aptamers. The NECEEM-based method can be used for discovery and characterization of drug candidates and the development of new diagnostic methods.
申请人：Sergey Krylov,Svetlana Krylova,Maxim Berezovski
地址：Concord CA,Concord CA,Toronto CA
国籍：CA,CA,CA
更多信息请下载全文后查看。