2003年上海交大生化期末试卷1※2

上海交通大学生物化学试卷（2003年度）校名___________ 系名___________ 专业___________姓名___________ 学号___________ 日期___________2003 TEST PAPER (1)（闭卷部分）(Shanghai Jiao Tong University,1st term of Univ. year 2003) Subject: Biochemistry Name _____________ Score _____________Class _____________一. Explain the terms:（2.5 x 10= 25）1.hyperuricemia2. LDL receptor3.pyruvate dehydrogenase complex4.glucokinase (in liver)5.malate-aspartate shuttle6.PRPP7.substrate cycle8.“macro CoA”9.urea cycle10. UDP- glucose2003 TEST PAPER (2)（开卷）(Shanghai Jiao Tong University,1st term of Univ. year 2003)Subject: Biochemistry Name _____________ Score _____________Class _____________二. Filling the blanks: (2.5 x 12= 30)1.Many important coenzymes, such as____, ____and ____, are the derivativesof ADP; dNDP are synthesized from ____ and dTMP is formed by________of dUMP; all these facts reflects that_____ came before______ in evolution.2.TPP is the derivative of vitamin ____, it serves as the prosthetic group of thefollowing enzymes: _____________, _____________and_____________.3.There is a large difference between the yield from the complete oxidation oftriacylglycerols and of carbohydrates (and protein), the reason is___________________________________________________.4.The three pairs of enzymes that catalyze the three resemble steps in β-oxidation of FA and citric acid cycle are_____________ and_____________, _____________and_____________, _____________and_____________.5.The only reactions in mammals that require coenzyme B12are__________________________ and _______________________catalyzed by _____________ and _____________, respectively.6.OAA has several possible fates: _____________, _____________,_____________ and _____________.7.In the synthesis of phosphoglycerides磷酸甘油酯and of glycogen, theactivated intermediates are _____________ and_____________, both ofthem are formed from a _____________ substrate and a _____________,and then react with a _____________ group.8.The committed step in cholesterol biosynthesis is the synthesis of_____________ from _____________which is catalyzed by _____________.9.The carrier of the activated one-carbon units is_____________ whichconsists of three groups: _____________, _____________and_____________. Besides, _____________ is the major donor of methylgroup. The fully oxidized one-carbon unit is _____________, which iscarried by_____________.10.Disease and inherited defects of enzyme or functional protein, e.g. PKU—Phe hydroxylase; drug-induced hemolytic anemia溶血性贫血—_____________; hypercholesterolemia高胆固醇血症—_____________;pernicious anemia恶性贫血—_____________; maple syrup槭糖浆urinedisease—_____________; Leber’s optic neuropathy视神经症—_____________.11.Acetyl CoA carboxylase from E. coli consists of three subunits called_____________, _____________ and _____________.12.There are two types of covalent modification: _____________and_____________in the regulation of enzymatic activity, the typicalexample of which is_____________ and _____________(enzyme), the directrelated residue is______ and _______, respectively.三．Make choice (one or several)：(3 x 8= 24)1. Schiff base intermediates are formed in the reactions catalyzed byA. aldolaseB. glycogen phosphorylaseC. Trp synthetaseD. transaldolaseE. aminotransferase2.Multifunctional (or bifunctional) enzyme occurs inA. pyrimidine synthesis of eukaryotes b. FA synthesis of eukaryotes C.the synthesis and hydrolysis of F-2,6-BP D. the phosphorylation anddephosphorylation of isocitrate DH E. FA synthesis of E. coli3.PRPP participates in the synthesis of the following substanceA. ProB. HisC. purineD. PheE. pyrimidine4.Biotin, as the posthetic group of the following enzymeA. acetyl CoA carboxylaseB. transketolaseC. pyruvate carboxylaseD. Pyr DHE. α–KG DH5.The following complex contains Fe-S clusterA. NADH-Q reductaseB. cytochrome c reductaseC. cytochrome coxidase D. succinate-Q reductase E. ribonucleotide reductase6.Which substance is the direct precursor of the synthesis of acetoacetate乙酰乙酸in liverA. HMG CoAB. acetoacetyl CoAC. D-3-hydroxybutyrate羟丁酸D. D-3-hydroxybutyryl CoAE. acetone7.Citric acid cycleA. it can produce high-energy phosphoryl compound by itselfB. it is notinhibited by the anaerobic厌氧condition C. the two carbon atoms ofacetyl CoA are liberated as the form of two CO2 in that round of the cycleD. its rate depends upon the need of ATPE. it is the final commonpathway for the oxidation of fuel molecules.8.It participates in the activated methyl cycle (or S-A Met cycle)A. VB12B. biotinC. FH4D. VB2E. nicotinate烟酸四. Answer or calculate: (7x 3= 21)1.What is the activated reactant in each of these biosyntheses?a)Sphingomyelin鞘磷脂from ceramide神经酰胺b)Met from homocysteine高半胱氨酸c)Phosphatidyl choline磷脂酰胆碱from phosphatidyl ethanolamine乙醇胺d)IPP from 5-pyrophosphomevalonate焦磷酸甲羟戊酸e)The synthesis of phosphoribosylamine磷酸核糖胺f)Ser from Glyg)Glycogen n+1 from glycogen n2. In purine biosynthesis, Asp becomes linked to the carboxylate group of anintermediate. In the next reaction, the carbon skeleton of the Asp unit leavesin the form of fumurate延胡索酸. These reactions are reminiscent重演ofa similar pair of reactions in the degradation of AA. Which ones?3. Currency exchange. For a proton-motive force of 0.2V (matrix negative),what is the maximum [ATP]/[ADP][Pi] ratio compatible with ATPsynthesis? Calculate this ratio three times, assuming that the number ofprotons translocated per ATP formed is 2, 3, and 4 and that the temperatureis 250C.2003 TEST PAPER (1) 答案一.1.hyperuricemia 高尿酸血血液中存在异常高含量的尿酸，可能由嘌呤合成增加引起。