流式细胞分析操作流程
合集下载
相关主题
- 1、下载文档前请自行甄别文档内容的完整性，平台不提供额外的编辑、内容补充、找答案等附加服务。
- 2、"仅部分预览"的文档,不可在线预览部分如存在完整性等问题,可反馈申请退款(可完整预览的文档不适用该条件!)。
- 3、如文档侵犯您的权益，请联系客服反馈,我们会尽快为您处理(人工客服工作时间：9:00-18:30)。
Cell Stem Cell 2007;1:313–323
Cell Stem Cell 2007;1:313–323
Cell Stem Cell 2007;1:313–323
Cell Stem Cell 2007;1:313–323
Cell Stem Cell 2007;1:313–323
Cell Stem Cell 2007;1:313–323
Cell Death and Differentiation 2008;15:51–57
Prolonged Maturation and Enhanced Transduction of Dendritic Cells Migrated from Human Skin Explants After In Situ Delivery of CD40-Targeted Adenoviral Vectors
J. Clin. Invest. 2007;117:902–909
J. Clin. Invest. 2007;117:902–909
J. Clin. Invest. 2007;117:902–909
Monocyte retention in the bone marrow
J. Clin. Invest. 2007;117:902–909
Histogram
CXCL12 and Vascular Endothelial Growth Factor Synergistically Induce Neoangiogenesis in Human Ovarian Cancers
Cancer Res 2005; 65(2): 465-72
Histogram
Science 2003;299:1057-1061
Contour
Imatinib potentiates antitumor T cell responses in gastrointestinal stromal tumor through the inhibition of Ido
Nature Medicine 2011；17（9）:1094-1100
Immunity 2011;35, 249–259
Identification and Functional Analysis of Tumor-Infiltrating Plasmacytoid Dendritic Cells in Head and Neck Cancer
Cancer Research 2003；63：6478–6487
Nature 2002；418:41-49
Histogram
Human dendritic cell mediated cytotoxicity against breast carcinoma cells in vitro
Journal of Leukocyte Biology 2002;72:312-320
Combined Genetic Inactivation of b2-microglobulin and CD58 Reveals Frequent Escape from Immune Recognition in Diffuse Large B Cell Lymphoma
Cancer Cell 2011；20： 728–740
Activated human umbilical cord blood dendritic cells kill tumor cells without damaging normal hematological progenitor cells
Cancer Sci 2005;96(2):127–133
JEM 2007；204（8）：1923-1933
The Journal of Immunology, 2002, 169: 2756–2761
Dot Plot
Breast cancer instructs dendritic cells to prime interleukin 13– secreting CD4+ T cells that facilitate tumor development IL-10 Elicits IFNg –Dependent Tumor Immune Surveillance
Cell Stem Cell 2007;1:313–323
Cell Stem Cell 2007;1:313–323
Histogram
Cancer Cell 2011；20： 728–740
Dot Plot
Senescence surveillance of pre-malignant hepatocytes limits liver cancer development
Nature 2011；479:547-551
Cell Stem Cell 2009;4:263–274
CD8 a+ Dendritic Cells Are the Critical Source of Interleukin-12 that Controls Acute Infection by Toxoplasma gondii Tachyzoites
JEM 2011;208(5):1069-1082
Plasmacytoid dendritic cells sense self-DNA coupled with antimicrobial peptide
Nature 2007;449:564-569
Expansion of myeloid immune suppressor Gr+CD11b+cells in tumor-bearing host directly promotes tumor angiogenesis
Cancer Res 2005; 65(8): 3437-46
Pretransplant CSF-1 therapy expands recipient macrophages and ameliorates GVHD after allogeneic hematopoietic cell transplantation
流式细胞术分析
CONTENTS
Histogram Dot Plots Contour Pseudo 3D & 3D
Gating
Papers Cell cycle Apoptosis
Histogram
Pluripotency of mesenchymal stem cells derived from adult marrow
Cancer Immunol Immunother 2007; 56:641–648
VCAM-1 Promotes Osteolytic Expansion of Indolent Bone Micrometastasis of Breast Cancer by Engaging a4b1PositiveOsteoclast Progenitors
Critical roles for CCR2 and MCP-3 in monocyte mobilization from bone marrow and recruitment to inflammatory sites
J. CFra Baidu bibliotekin. Invest. 2007;117:902–909
50% monocytes 20%–30% B cells 10%–20% T cells
Cancer cell 2004;6:409-421
Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and Treg cells
Nature 2011；475:226-230
Nonmyelinating Schwann Cells Maintain Hematopoietic Stem Cell Hibernation in the Bone Marrow Niche
Cancer Res 2006; 66(13): 6807-15
Flt-1, vascular endothelial growth factor receptor 1, is a novel cell surface marker for the lineage of monocyte-macrophages in humans Blood. 2001;97:785-791
Contour
Antiangiogenic and Antimetastatic Activity of JAK Inhibitor AZD1480
Cancer Res 2011; 71(21); 6601–10
Pseudo 3D & 3D
Gating
Peripheral blood
CD80 in Immune Suppression by Mouse Ovarian Carcinoma– Associated Gr-1+ CD11b+ Myeloid Cells
Essential Role of Lung Plasmacytoid Dendritic Cells in Preventing Asthmatic Reactions to Harmless Inhaled Antigen
J. Exp. Med 2004;200(1):89-98
Metronomic cyclophosphamide regimen selectively depletes CD4+CD25+ regulatory T cells and restores T and NK effector functions in end stage cancer patients
J. Clin. Invest. 2007;117:902–909
Distinct Populations of Cancer Stem Cells Determine Tumor Growth and Metastatic Activity in Human Pancreatic Cancer
The Journal of Immunology, 2002, 169: 5322–5331
Redirecting In vivo Elicited Tumor Infiltrating Macrophages and Dendritic Cells towards Tumor Rejection
Cancer Cell 2011；20：781–796
JEM 2007；204（5）：1037–1047 The Journal of Immunology, 2009, 182: 240–249
Dot Plot
Control of Regulatory T Cell Development by the Transcription Factor Foxp3
Dot Plot
Prevalence of Regulatory T Cells Is Increased in Peripheral Blood and Tumor Microenvironment of Patients with Pancreas or Breast Adenocarcinoma
Cancer Cell 2011; 20, 701–714
Engraftment and Reconstitution of Hematopoiesis Is Dependent on VEGFR2-Mediated Regeneration of Sinusoidal Endothelial Cells
Cell 2011；147：1146–1158
Oncogene-Targeting T Cells Reject Large Tumors while Oncogene Inactivation Selects Escape Variants in Mouse Models of Cancer
Cancer Cell 2011;20:755–767
Cell Stem Cell 2007;1:313–323
Cell Stem Cell 2007;1:313–323
Cell Stem Cell 2007;1:313–323
Cell Stem Cell 2007;1:313–323
Cell Stem Cell 2007;1:313–323
Cell Death and Differentiation 2008;15:51–57
Prolonged Maturation and Enhanced Transduction of Dendritic Cells Migrated from Human Skin Explants After In Situ Delivery of CD40-Targeted Adenoviral Vectors
J. Clin. Invest. 2007;117:902–909
J. Clin. Invest. 2007;117:902–909
J. Clin. Invest. 2007;117:902–909
Monocyte retention in the bone marrow
J. Clin. Invest. 2007;117:902–909
Histogram
CXCL12 and Vascular Endothelial Growth Factor Synergistically Induce Neoangiogenesis in Human Ovarian Cancers
Cancer Res 2005; 65(2): 465-72
Histogram
Science 2003;299:1057-1061
Contour
Imatinib potentiates antitumor T cell responses in gastrointestinal stromal tumor through the inhibition of Ido
Nature Medicine 2011；17（9）:1094-1100
Immunity 2011;35, 249–259
Identification and Functional Analysis of Tumor-Infiltrating Plasmacytoid Dendritic Cells in Head and Neck Cancer
Cancer Research 2003；63：6478–6487
Nature 2002；418:41-49
Histogram
Human dendritic cell mediated cytotoxicity against breast carcinoma cells in vitro
Journal of Leukocyte Biology 2002;72:312-320
Combined Genetic Inactivation of b2-microglobulin and CD58 Reveals Frequent Escape from Immune Recognition in Diffuse Large B Cell Lymphoma
Cancer Cell 2011；20： 728–740
Activated human umbilical cord blood dendritic cells kill tumor cells without damaging normal hematological progenitor cells
Cancer Sci 2005;96(2):127–133
JEM 2007；204（8）：1923-1933
The Journal of Immunology, 2002, 169: 2756–2761
Dot Plot
Breast cancer instructs dendritic cells to prime interleukin 13– secreting CD4+ T cells that facilitate tumor development IL-10 Elicits IFNg –Dependent Tumor Immune Surveillance
Cell Stem Cell 2007;1:313–323
Cell Stem Cell 2007;1:313–323
Histogram
Cancer Cell 2011；20： 728–740
Dot Plot
Senescence surveillance of pre-malignant hepatocytes limits liver cancer development
Nature 2011；479:547-551
Cell Stem Cell 2009;4:263–274
CD8 a+ Dendritic Cells Are the Critical Source of Interleukin-12 that Controls Acute Infection by Toxoplasma gondii Tachyzoites
JEM 2011;208(5):1069-1082
Plasmacytoid dendritic cells sense self-DNA coupled with antimicrobial peptide
Nature 2007;449:564-569
Expansion of myeloid immune suppressor Gr+CD11b+cells in tumor-bearing host directly promotes tumor angiogenesis
Cancer Res 2005; 65(8): 3437-46
Pretransplant CSF-1 therapy expands recipient macrophages and ameliorates GVHD after allogeneic hematopoietic cell transplantation
流式细胞术分析
CONTENTS
Histogram Dot Plots Contour Pseudo 3D & 3D
Gating
Papers Cell cycle Apoptosis
Histogram
Pluripotency of mesenchymal stem cells derived from adult marrow
Cancer Immunol Immunother 2007; 56:641–648
VCAM-1 Promotes Osteolytic Expansion of Indolent Bone Micrometastasis of Breast Cancer by Engaging a4b1PositiveOsteoclast Progenitors
Critical roles for CCR2 and MCP-3 in monocyte mobilization from bone marrow and recruitment to inflammatory sites
J. CFra Baidu bibliotekin. Invest. 2007;117:902–909
50% monocytes 20%–30% B cells 10%–20% T cells
Cancer cell 2004;6:409-421
Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and Treg cells
Nature 2011；475:226-230
Nonmyelinating Schwann Cells Maintain Hematopoietic Stem Cell Hibernation in the Bone Marrow Niche
Cancer Res 2006; 66(13): 6807-15
Flt-1, vascular endothelial growth factor receptor 1, is a novel cell surface marker for the lineage of monocyte-macrophages in humans Blood. 2001;97:785-791
Contour
Antiangiogenic and Antimetastatic Activity of JAK Inhibitor AZD1480
Cancer Res 2011; 71(21); 6601–10
Pseudo 3D & 3D
Gating
Peripheral blood
CD80 in Immune Suppression by Mouse Ovarian Carcinoma– Associated Gr-1+ CD11b+ Myeloid Cells
Essential Role of Lung Plasmacytoid Dendritic Cells in Preventing Asthmatic Reactions to Harmless Inhaled Antigen
J. Exp. Med 2004;200(1):89-98
Metronomic cyclophosphamide regimen selectively depletes CD4+CD25+ regulatory T cells and restores T and NK effector functions in end stage cancer patients
J. Clin. Invest. 2007;117:902–909
Distinct Populations of Cancer Stem Cells Determine Tumor Growth and Metastatic Activity in Human Pancreatic Cancer
The Journal of Immunology, 2002, 169: 5322–5331
Redirecting In vivo Elicited Tumor Infiltrating Macrophages and Dendritic Cells towards Tumor Rejection
Cancer Cell 2011；20：781–796
JEM 2007；204（5）：1037–1047 The Journal of Immunology, 2009, 182: 240–249
Dot Plot
Control of Regulatory T Cell Development by the Transcription Factor Foxp3
Dot Plot
Prevalence of Regulatory T Cells Is Increased in Peripheral Blood and Tumor Microenvironment of Patients with Pancreas or Breast Adenocarcinoma
Cancer Cell 2011; 20, 701–714
Engraftment and Reconstitution of Hematopoiesis Is Dependent on VEGFR2-Mediated Regeneration of Sinusoidal Endothelial Cells
Cell 2011；147：1146–1158
Oncogene-Targeting T Cells Reject Large Tumors while Oncogene Inactivation Selects Escape Variants in Mouse Models of Cancer
Cancer Cell 2011;20:755–767