地黄栽培种与野生种内源激素含量的差异

地黄栽培种与野生Leabharlann Baidu内源激素含量的差异
李先恩1,2
孙 鹏2
祁建军2
周丽莉2
王绍华1，*
1 南京农业大学农学院，江苏南京 210095;2 中国医学科学院北京协和医学院药用植物研究所，北京 100094
摘 要：块根的发育和形成与植物内源激素密切相关。本研究测定地黄2个栽培种和2个野生种在生长发育过程中 内源激素含量表明，地黄叶片中IAA、ZR和ABA含量变化均呈单峰曲线，即在拉线期及快速增长期逐渐升高，之后 逐渐降低，但各生育期栽培种叶片中IAA、ZR和ABA含量均高于野生种；栽培种与野生种块根中IAA和ZR含量 变化规律明显不同，在拉线期与快速增长期，栽培种迅速上升，而后逐渐降低，而在整个生长期野生种变化不明显； 整个生育期参试品种叶片中GA含量变化不大，栽培种在快速增长期根中GA、ABA的含量迅速上升，而野生种变化 不明显。从上述变化规律来看，地黄块根的形成与发育是多种内源激素协同作用的结果。IAA和ZR是块根起始分化 关键激素，决定了块根的形成，GA、ABA在地黄块根的发育与膨大中起了更重要的作用。 关键词：地黄；栽培种；野生种；植物激素
作物学报 ACTA AGRONOMICA SINICA 2013，39（7）：1276-1283 ISSN 0496-3490；CODEN TSHPA9
D O I：10.3724/SP.J.1006.2013.01276
http：//www.chinacrops.org/zwxb/ E-mail:xbzw@chinajournal.net.cn
A bstract:The form ation and developm ent of tuberous roots is related to plant endogenous horm ones.We determined the contents of IAA ,ZR ,G A ,and ABA in leaves and roots of two cultivars and two wild-type varieties of Rehmannia glutinosa during the grow th periods and com pared their developm ental changing trends between cultivars and wild-type varieties of Rehmannia glutinosa.The results show ed that the contents of IAA ,ZR ,and ABA in leaves of cultivated Rehm annia glutinosa were higher than those of wild-type varieties and their changes in both cultivated and wild type of R.glutinosa displayed the saine single-peak curves during the grow th periods,they were all increased gradually at the elongation and rapid enlargem ent stages of roots and then decreased gradually.There was no significant developm ental change in GA content in the leaves of both cultivated and w ild-type varieties.H orm one contents in roots of cultivars had quite different developm ental changes from those of wild-type varieties.The contents of IAA ,ZR ,and ABA in the cultivars presented the changing trend of single-peak curves during root grow th periods while GA content increased rapidly in root enlargem ent stage.There was no much developm ental change in IAA and GA contents in roots of wild-type varieties.ZR content decreased gradually with the root developm ent in wild-type varieties. A BA content changed in roots of wild-type varieties displayed a single-peak curve which was much low er than that of the cultivars.This different developm ental changes of horm one contents in rehm annia roots between cultivars and wild-type varieties indicated that the developm ent of the rehm annia tuberous roots is affected by synergitic effects of several horm ones and that IAA and ZR act on the initiation of tuberous roots,w hereas GA and ABA play a more im portant role in root enlargem ent and thickening. K eyw ords:Rehmannia glutinosa Libosch;Cultivar;W ild-type varieties;H orm ones
Ch a n g e s of H o r mon e s in C u ltiv a r s an d tin o s a L ib o sc h .
Wild -T y p e V a r ieties of R e h man n ia g lu -
LI Xian-E n , 1，2 SU N Peng2,Q I Jian-Jun2,Z H O U Li-Li2,and WA N G Shao-H ua1,* 1 College of Agriculture,Nanjing Agricultural University,Nanjing 210095,China;2 Institute of Medicinal Plant Development,China Academy of Medical Sciences & Peking Union Medical College,Beijing 100094,China