犬瘟热金标快速检测试纸条(CDV-Ag)

犬瘟热金标快速检测试纸条（CDV-Ag）

简介

犬瘟热，俗称狗瘟，是由犬瘟热病毒引起的一种烈性传染病。临床特征为双相热型、消化道、呼吸道炎症，眼、鼻流出脓样分泌物，少数病例可发生非化脓性脑炎。多发生于3-6个月龄幼犬，青年犬也有感染，潜伏期为3-6天。病毒单独感染症状轻微，若继发细菌感染症状加重，病死率较高。

检测原理

本试纸为免疫层析法对病毒进行双抗体夹心检测。检测样品为眼部及结膜分泌物，鼻液，唾液。产品组分

1．20份CDV Ag 试纸卡

2．20份样品稀释液

3．20份消毒棉签

4．一份产品说明书

保存及有效期限

室温保存，不可冷冻。生产日期起18个月有效。

样品收集及准备

1、用生理盐水沾湿的棉签收集狗眼部及结膜分泌物，鼻液，唾液；

2、采集样品时注意多部位同时收集新鲜及有效样品，充分在试管中搅拌稀释；；

3、静置5分钟，用一次性滴管取上清液；

4、样品一般须当即进行检测，否则应冷藏保存，超过24小时的，应该冷冻保存。

检测步骤

1、试纸条恢复室温；

2、取出试纸，开封后平放在桌面，从滴管中缓慢而准确地逐滴加入3-5滴混合液。

3、加样品液后，红色的液体从靠样品孔的观察窗边缘涌出，朝另一方向流动。

4、5-10分钟后判断结果。

结果判定

阳性（+）：当位置C显示出红色线条，而位置T同时显示出红色线条时，判为阳性。

阴性（-）：当位置C显示出红色线条，而位置T不显色时，判为阴性。

无效：当位置C不显示出红色线条，则无论位置T显示出红色线条与否，该试剂盒判为无效。

阳性阴性

注意事项

1．仅用作体外诊断。

2．注意样品具有潜在传染性，注意防止交叉感染。

3．该试纸及配套试管、棉签均为一次性产品，不可交叉及重复使用。

4．包装袋破损或产品过期请勿使用。

Canine Distemper Virus Rapid Test Card

Introduce

Canine distemper is caused by the canine distemper virus (CDV).It is a contagious, incurable, often fatal, multisystemic viral disease that affects the respiratory, gastrointestinal, and central nervous systems. Infected dogs shed the virus through bodily secretions and excretions, especially respiratory secretions. Young puppies between 3 and 6 months old are most susceptible to infection and disease and are more likely to die than infected adults. Principle

The test card is based on immunochromatography of double-antibody sandwich. Testing sample is the eye and conjunctival secretions,nasal fluid.

Content

1 CDV diagnostic Card: 20 pcs

2 Dilution Buffer: 20 pcs

3 Swab: 20 pcs

4 Instruction: 1 insert

Procedure

1. Recover the dipstick to room temperature. remove as many of the test devices from the foil pouches as needed for testing.

ing the disposable sample collection swab take a portion of secretion of eye mucus,nasal fluid.insert the collection swab into specimen tube containing the sample diluent.

3.Mix the swab until the sample has been dissolved into the sample diluent.Discard the swab and repeat procedure for each sample. Adding 4 drops containing the sample diluent into the sample-well on the card.

4. Read results after 10 minutes.

Results

Positive:The test zone and control zone have a pink/purple band visible.This indicates that the presence of canine distemper virus antigens.

Negative:Only the control zone has a pink/purple band visible.The sample do not contains canine distemper virus antigens.

Void:No color reaction on C line.

Positive Negative

Precautions

− Handle all biological materials as though capable of transmitting CDV.

− Do not eat, drink, smoke or prepare foods, or apply cosmetics within the designated work area.

− Do not use components past expiration date.

− Optimal results will be obtained by strict adherence to this protocol. Careful washing and pipetting throughout this procedure are necessary to maintain precision and accuracy.

− Do not use components past expiration date.

− Only for veterinary use.

The entire risk as to the performance of these products is assumed by the purchaser. We shall not be liable for indirect,special or consequential damages of any kind resulting from use of the products