2013年《微生物学》实验双语测试题

《微生物学》实验双语测试题

《Experiment for microbiology》test

1.Multiple Choice (choose one answer)（每小题2分，共20分）

1.The first microscopes were developed by:

A.Ehrlich

B.Metchnikoff

C.Leewenhoek

D.Lister

2.Light microscopy is dependent on the interaction of light with on object. The ability of light to pass through an object is referred to as:

A.transported light

B.transmitted light

C.reflected light

D.refracted light

3.The resolving power (R) of a microscope is dependent on the wavelength of light (;~) and the numerical aperture (NA) of the lens. The formula for R is:

A.R = 0.5~. xNA

B.R = 0.5;~/NA

C.R = NA/0.5Jr

D.R = Square root of 0.5)./NA

4.The gram stain uses ~ as a mordant to fix the primary stain:

A.iodine

B.alcohol

C.acetone

D.safranin

5.The acid-fast stain is useful in the identification of which of the following organisms:

A.Staphylococcus aureus

B.Mycoplasma mycoides

C.Mycobacteria tuberculosis

D.Moraxella osloensis

6.Which of the following types of microscopes utilizes electron beams to visualize objects?

A.Nomarski

B.TEM

C.PCM

D.Confocal

7.Sterilization of material with an autoclave utilizes steam to kill microorganisms. The correct procedure for sterilization with an autoclave is:

A.15 min at 121℃at 15 lb/in2

B.15 min at 256℃at 15 lb/in2

C.15 min at 121℃at 1 lb/in2

D.15 rain at 121℃at 30 lb/in2

8.An antibiotic was added to a culture of bacteria to determine its effect. What method of

enumeration would you use to determine the efficacy of the antibiotic?

A.direct count

B.viable count

C.turbidimetric count

D.absorbance

9.The transfer of DNA from one organism to another through the use of a viral vector is referred to as:

A.electroporation

B.conjugation

C.transformation

D.transduction

10. In bacterial cultures, growth can be demonstrated by an increase in:

A.mass

B.cell size

C.cell number

D.cell length

得分

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2．Fill in the Blank（每小题1分，共20分）

1. The structure of the bright-field microscope

Fig 1 Bight-field microscope

1. Ocular lens.

2. Tube.

3. Nosepiece.

4. Objective lens.

5. Stage.

6. Condenser lens.

7. Iris diaphragm.

8. Reflector.

9. Arm. 10. Stage adjustment knob. 11. Condenser adjustment knob. 12. Fine-adjustment knob. 13.Coarse-adjustment knob. 14. Base.

2. IMViC Series include 15.Indole production、16.The Methyl Red Test、17.The Voges-Proskauer Test、18.Citrate Utilization

3. Pasteurization Inoculate Bacillus subtilis and Escherichia coli respectively to nutrient broth, place the tubes to a 63℃water bath for 30 minutes.

得分

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3．Matching（每小题2分，共24分）

Matching I:

l. Primary stain for gram stain a. Negative stain

2. Stains bacterial cell b. Carbohl fuchsin

3. Used to fix stain c. Crystal violet