常用酶切位点表含保护碱基
合集下载
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10
10
Sac II
GCCGCGG C
0
0
TCC CCGCGG GGA
50
>90
Sal I
GTCGAC GTCAAAAGGCCATAGCGGCCGC
0
0
GC GTCGAC GTCTTGGCCATAGCGGCCGCG
10
50
G
10
75
ACGC GTCGAC GTCGGCCATAGCGGCCGCG
GAA
酶
寡核苷酸序列
切割率%
2 hr
20 hr
Acc I
G GTCGAC C
0
0
CG GTCGAC CG
0
0
CCG GTCGAC CGG
0
0
Afl III
CACATGT G
0
0
CCACATGT GG
>90
>90
CCC ACATGT GGG
>90
>90
Asc I
GGCGCGCC
>90
>90
AGGCGCGCC T
>90
>90
10
10
AA CTGCAG AACCAATGCATTGG
>90
>90
AAAA CTGCAG CCAATGCATTGGAA
>90
>90
CTGCAG AACCAATGCATTGGATGCAT
0
0
Pvu I
CCGATCG G
0
0
AT CGATCG AT
10
25
TCG CGATCG CGA
0
10
Sac I
CGAGCTC G
0
0
GGAATTC CATATG GAATTCC
75
>90
GGGAATTC CATATG GAATTCCC
75
>90
Nhe I
GGCTAGC C
0
0
CG GCTAGC CG
10
25
CTA GCTAGC TAG
10
50
酶
寡核苷酸序列
切割率%
2 hr
20 hr
Not I
TTGCGGCCGC AA
0
0
ATTT GCGGCCGC TTTA
CTAG ACTAGT CTAG
0
50
Sph I
GGCATGC C
0
0
CAT GCATGC ATG
0
25
ACAT GCATGC ATGT
10
50
Stu I
AAGGCCT T
>90
>90
GAAGGCCT TC
Biblioteka Baidu>90
>90
AAA AGGCCT TTT
>90
>90
Xba I
CTCTAGA G
0
0
GC TCTAGA GC
>90
>90
TTGGCGCGCC AA
>90
>90
Ava I
CCCCGGG G
50
>90
CCCCCGGG GG
>90
>90
TCC CCCGGG GGA
>90
>90
BamH I
CGGATCC G
10
25
CG GGATCC CG
>90
>90
CGC GGATCC GCG
>90
>90
Bgl II
CAGATCT G
0
0
GAAGATCT TC
75
>90
GGA AGATCT TCC
25
>90
BssH II
GGCGCGC C
0
0
AG GCGCGC CT
0
0
TTG GCGCGC CAA
50
>90
BstE II
GGGT(A/T)ACC C
0
10
BstX I
AACTGCAGAA CCAATGCATTGG
0
0
AAAACTGCAG CCAATGCATTGG AA
TTAATTAA
0
0
GTTAATTAA C
0
25
CC TTAATTAA GG
0
>90
Pme I
0
0
GTTTAAAC
GGTTTAAAC C
0
25
GG GTTTAAAC CC
0
50
AGCTTT GTTTAAAC GGCGCGCCGG
75
>90
Pst I
GCTGCAG C
0
0
TGCA CTGCAG TGCA
CGG GGTACC CCG
>90
>90
Mlu I
GACGCGT C
0
0
CGACGCGT CG
25
50
Neo 1
CCCATGG G
0
0
CATG CCATGG CATG
50
75
Nde I
CCATATG G
0
0
CC CATATG GG
0
0
CGC CATATG GCG
0
0
GGGTTT CATATG AAACCC
Sea I
GAGTACT C
10
25
AAA AGTACT TTT
75
75
Sma I
CCCGGG
0
10
CCCCGGG G
0
10
CCCCCGGG GG
10
50
TCC CCCGGG GGA
>90
>90
Spe I
GACTAGT C
10
>90
GG ACTAGT CC
10
>90
CGG ACTAGT CCG
0
50
>90
>90
Hae III
GG GGCC CC
>90
>90
AGC GGCC GCT
>90
>90
TTGC GGCC GCAA
>90
>90
Hind III
CAAGCTT G
0
0
CCAAGCTT GG
0
0
CCC AAGCTT GGG
10
75
Kpn I
GGGTACC C
0
0
GGGGTACC CC
>90
>90
10
10
AAATAT GCGGCCGC TATAAA
10
10
ATAAGAAT GCGGCCGC TAAACTAT
25
90
AAGGAAAAAA GCGGCCGC AAAAGGAAAA
25
>90
Nsi I
TGC ATGCAT GCA
10
>90
CCA ATGCAT TGGTTCTGCAGTT
>90
>90
Pae I
25
50
CTGCAGAA CCAATGCATTGG ATGCAT
25
>90
Cla I
CATCGAT G
0
0
GATCGAT C
0
0
CCATCGAT GG
>90
>90
CCC ATCGAT GGG
50
50
EcoR I
GGAATTC C
>90
>90
CG GAATTC CG
>90
>90
CCG GAATTC CGG
>90
>90
TGC TCTAGA GCA
75
>90
CTAG TCTAGA CTAG
75
>90
Xho I
CCTCGAG G
0
0
CC CTCGAG GG
10
25
CCGCTCGAG CGG
10
75
Xma I
CCCCGGG G
0
0
CCCCCGGG GG
25
75
CCC CCCGGG GGG
50
>90
TCCC CCCGGG GGGA
10
Sac II
GCCGCGG C
0
0
TCC CCGCGG GGA
50
>90
Sal I
GTCGAC GTCAAAAGGCCATAGCGGCCGC
0
0
GC GTCGAC GTCTTGGCCATAGCGGCCGCG
10
50
G
10
75
ACGC GTCGAC GTCGGCCATAGCGGCCGCG
GAA
酶
寡核苷酸序列
切割率%
2 hr
20 hr
Acc I
G GTCGAC C
0
0
CG GTCGAC CG
0
0
CCG GTCGAC CGG
0
0
Afl III
CACATGT G
0
0
CCACATGT GG
>90
>90
CCC ACATGT GGG
>90
>90
Asc I
GGCGCGCC
>90
>90
AGGCGCGCC T
>90
>90
10
10
AA CTGCAG AACCAATGCATTGG
>90
>90
AAAA CTGCAG CCAATGCATTGGAA
>90
>90
CTGCAG AACCAATGCATTGGATGCAT
0
0
Pvu I
CCGATCG G
0
0
AT CGATCG AT
10
25
TCG CGATCG CGA
0
10
Sac I
CGAGCTC G
0
0
GGAATTC CATATG GAATTCC
75
>90
GGGAATTC CATATG GAATTCCC
75
>90
Nhe I
GGCTAGC C
0
0
CG GCTAGC CG
10
25
CTA GCTAGC TAG
10
50
酶
寡核苷酸序列
切割率%
2 hr
20 hr
Not I
TTGCGGCCGC AA
0
0
ATTT GCGGCCGC TTTA
CTAG ACTAGT CTAG
0
50
Sph I
GGCATGC C
0
0
CAT GCATGC ATG
0
25
ACAT GCATGC ATGT
10
50
Stu I
AAGGCCT T
>90
>90
GAAGGCCT TC
Biblioteka Baidu>90
>90
AAA AGGCCT TTT
>90
>90
Xba I
CTCTAGA G
0
0
GC TCTAGA GC
>90
>90
TTGGCGCGCC AA
>90
>90
Ava I
CCCCGGG G
50
>90
CCCCCGGG GG
>90
>90
TCC CCCGGG GGA
>90
>90
BamH I
CGGATCC G
10
25
CG GGATCC CG
>90
>90
CGC GGATCC GCG
>90
>90
Bgl II
CAGATCT G
0
0
GAAGATCT TC
75
>90
GGA AGATCT TCC
25
>90
BssH II
GGCGCGC C
0
0
AG GCGCGC CT
0
0
TTG GCGCGC CAA
50
>90
BstE II
GGGT(A/T)ACC C
0
10
BstX I
AACTGCAGAA CCAATGCATTGG
0
0
AAAACTGCAG CCAATGCATTGG AA
TTAATTAA
0
0
GTTAATTAA C
0
25
CC TTAATTAA GG
0
>90
Pme I
0
0
GTTTAAAC
GGTTTAAAC C
0
25
GG GTTTAAAC CC
0
50
AGCTTT GTTTAAAC GGCGCGCCGG
75
>90
Pst I
GCTGCAG C
0
0
TGCA CTGCAG TGCA
CGG GGTACC CCG
>90
>90
Mlu I
GACGCGT C
0
0
CGACGCGT CG
25
50
Neo 1
CCCATGG G
0
0
CATG CCATGG CATG
50
75
Nde I
CCATATG G
0
0
CC CATATG GG
0
0
CGC CATATG GCG
0
0
GGGTTT CATATG AAACCC
Sea I
GAGTACT C
10
25
AAA AGTACT TTT
75
75
Sma I
CCCGGG
0
10
CCCCGGG G
0
10
CCCCCGGG GG
10
50
TCC CCCGGG GGA
>90
>90
Spe I
GACTAGT C
10
>90
GG ACTAGT CC
10
>90
CGG ACTAGT CCG
0
50
>90
>90
Hae III
GG GGCC CC
>90
>90
AGC GGCC GCT
>90
>90
TTGC GGCC GCAA
>90
>90
Hind III
CAAGCTT G
0
0
CCAAGCTT GG
0
0
CCC AAGCTT GGG
10
75
Kpn I
GGGTACC C
0
0
GGGGTACC CC
>90
>90
10
10
AAATAT GCGGCCGC TATAAA
10
10
ATAAGAAT GCGGCCGC TAAACTAT
25
90
AAGGAAAAAA GCGGCCGC AAAAGGAAAA
25
>90
Nsi I
TGC ATGCAT GCA
10
>90
CCA ATGCAT TGGTTCTGCAGTT
>90
>90
Pae I
25
50
CTGCAGAA CCAATGCATTGG ATGCAT
25
>90
Cla I
CATCGAT G
0
0
GATCGAT C
0
0
CCATCGAT GG
>90
>90
CCC ATCGAT GGG
50
50
EcoR I
GGAATTC C
>90
>90
CG GAATTC CG
>90
>90
CCG GAATTC CGG
>90
>90
TGC TCTAGA GCA
75
>90
CTAG TCTAGA CTAG
75
>90
Xho I
CCTCGAG G
0
0
CC CTCGAG GG
10
25
CCGCTCGAG CGG
10
75
Xma I
CCCCGGG G
0
0
CCCCCGGG GG
25
75
CCC CCCGGG GGG
50
>90
TCCC CCCGGG GGGA