癌症生物学(1)
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7. 隨時歡迎同學的口頭或書面建議
The Biology of Cancer
Robert A. Weinberg Garland Science, Taylor & Francis Group, LLC, 2007
Robert A. Weinberg
Whitehead Institute for Biomedical Research Massachusetts Institute of Technology (MIT)
癌症生物学(1)
2020/8/30
4. 對同學的期許:
a. 準時上課，如無法出席，須以電話(留言)、 e-mail、簡訊…請假
b. 閱讀英文教科書 c. 主動參與討論，表達自己的想法及看法 d. 分組口頭報告 (事先與老師討論) e. 對同學的報告提出問題、討論及評量 f. 隨堂考試、期中考試、期末考試
p centromere
q
Figure 1.11a The Biology of Cancer (© Garland Science 2007)
Chromosome Banding (1970s)
- Identification of chromosomes or the chromosome rearrangement in cells
Curing Cancer – M. Waldholz (1997) 致癌基因之謎 (商業週刊)
Chapter 1 The Biology and Genetics of Cells and
Organisms
- 1.4 - 1.5 -
Mar 1, 2007
Human Chromosomes (during metaphase)
Racing to the Beginning of the Road: The Search for the Origin of Cancer – R. A. Weinberg (1996) 追獵癌症：癌症病因研究之路 (天下文化)
One Renegade Cell – R. A. Weinberg (1998) 細胞反叛 (天下文化)
Q-banding is a fluorescent pattern obtained using quinacrine for staining. The pattern of bands is very similar to that seen in Gbanding.
p: the short arm above the centromere, e.g., p13, p23 q: the long arm below the centromere, e.g., q22, q32
The aneuploid karyotype of a human breast cancer cell
Figure 1.11c The Biology of Cancer (© Garland Science 2007)
Chromosomes may undergo changes in the structure :
5. 成績考核：
由多方面考核；如準時出席、上課反應、小組參與、 口頭報告、自發學習、隨堂考試、期中考及期末考
6. 教科書: Weinberg, R. A. The Biology of Cancer.
Garland Science, Taylor & Francis Group, LLC, 2007
C-banding stains centromeres.
R-banding is the reverse of C-banding and stains noncentromeric regions.
G-banding is obtained with Giemsa stain. It yields a series of lightly and darkly stained bands.
Sidebar 1.3 Cancer cells are often aneuploid
Euploidy – normal configuration (numbers & pairs) of chromosomes
Aneuploidy – changes in chromosome number → acquisition of extra copies of one chromosome or the loss of another
Cancer cells often exhibit: a. presence of extra copies of chromosome b. loss of entire chromosomes c. fusion of the arm of one chromosome with part of another d. aberrantly structured chromosomes
- The interaction of certain alkylating (adding - CH3) fluorochromes (e.g., quinacrine mustard) and histochemical stains (e.g., Giemsa stain) with specific regionຫໍສະໝຸດ Baidu of chromosomes produces “bands” along the chromosomes that can be used to fingerprint each chromosome pair. The patterns obtained depend on the treatment used.
The Diploid Karyotype of a Normal Cell
Figure 1.11b The Biology of Cancer (© Garland Science 2007)
1.4 Chromosomes are altered in most types of cancer cells
The Biology of Cancer
Robert A. Weinberg Garland Science, Taylor & Francis Group, LLC, 2007
Robert A. Weinberg
Whitehead Institute for Biomedical Research Massachusetts Institute of Technology (MIT)
癌症生物学(1)
2020/8/30
4. 對同學的期許:
a. 準時上課，如無法出席，須以電話(留言)、 e-mail、簡訊…請假
b. 閱讀英文教科書 c. 主動參與討論，表達自己的想法及看法 d. 分組口頭報告 (事先與老師討論) e. 對同學的報告提出問題、討論及評量 f. 隨堂考試、期中考試、期末考試
p centromere
q
Figure 1.11a The Biology of Cancer (© Garland Science 2007)
Chromosome Banding (1970s)
- Identification of chromosomes or the chromosome rearrangement in cells
Curing Cancer – M. Waldholz (1997) 致癌基因之謎 (商業週刊)
Chapter 1 The Biology and Genetics of Cells and
Organisms
- 1.4 - 1.5 -
Mar 1, 2007
Human Chromosomes (during metaphase)
Racing to the Beginning of the Road: The Search for the Origin of Cancer – R. A. Weinberg (1996) 追獵癌症：癌症病因研究之路 (天下文化)
One Renegade Cell – R. A. Weinberg (1998) 細胞反叛 (天下文化)
Q-banding is a fluorescent pattern obtained using quinacrine for staining. The pattern of bands is very similar to that seen in Gbanding.
p: the short arm above the centromere, e.g., p13, p23 q: the long arm below the centromere, e.g., q22, q32
The aneuploid karyotype of a human breast cancer cell
Figure 1.11c The Biology of Cancer (© Garland Science 2007)
Chromosomes may undergo changes in the structure :
5. 成績考核：
由多方面考核；如準時出席、上課反應、小組參與、 口頭報告、自發學習、隨堂考試、期中考及期末考
6. 教科書: Weinberg, R. A. The Biology of Cancer.
Garland Science, Taylor & Francis Group, LLC, 2007
C-banding stains centromeres.
R-banding is the reverse of C-banding and stains noncentromeric regions.
G-banding is obtained with Giemsa stain. It yields a series of lightly and darkly stained bands.
Sidebar 1.3 Cancer cells are often aneuploid
Euploidy – normal configuration (numbers & pairs) of chromosomes
Aneuploidy – changes in chromosome number → acquisition of extra copies of one chromosome or the loss of another
Cancer cells often exhibit: a. presence of extra copies of chromosome b. loss of entire chromosomes c. fusion of the arm of one chromosome with part of another d. aberrantly structured chromosomes
- The interaction of certain alkylating (adding - CH3) fluorochromes (e.g., quinacrine mustard) and histochemical stains (e.g., Giemsa stain) with specific regionຫໍສະໝຸດ Baidu of chromosomes produces “bands” along the chromosomes that can be used to fingerprint each chromosome pair. The patterns obtained depend on the treatment used.
The Diploid Karyotype of a Normal Cell
Figure 1.11b The Biology of Cancer (© Garland Science 2007)
1.4 Chromosomes are altered in most types of cancer cells