泰高在饲料和食品安全方面的研发工作

Swine Research Centre
Calf Research Centre
Ingredients Research Centre
Located in: Norway, Spain, Canada and The Netherlands Serving 3 business groups: Aquaculture, Agriculture, Specialties. And lots of synergies!
Multiplication Crop
Βιβλιοθήκη Baidu
Attachment mucosa Intestinal Multiplication Invasion enterocytes Systemic infection
Excretion via faeces
Gall-bladder
Liver
Spleen
Lungs 8
FARM Infection pressure from: One day old birds/rearing hens Housing system Contaminated feed/water system Excreting broilers/layers
6
High BC & fat content
3 1-2 1-2 4
Act via another mode of action than organic acids. Synergistic effects. C8 and C10 performed best.
Physico-chemical behaviour in blends and costs of etheric oils are a concern
泰高在饲料和食品安全方面的研发工作 Nutreco R & D on Feed and Food Safety
韩延明 Yanming Han Ingredient Research Centre Nutreco R & D, 荷兰 The Netherlands May 17, 2010, Beijing, China
High Salmonella prevalence
% Salmonella positive 1- 50% 10-20% 6,0 % 6.0 % 4.0 % 3.0 % 1- 4 % 1 – 4% < 1,0%
Critical raw material & Feed Meat meal Soybean meal full fat Rapeseed expeller Soybean meal expeller Rapeseed meal Soybean meal Layer feed Broiler feed Cereals
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Candidate Salmonella inhibitors
Compilation of in vitro results
Lead Ranking antimicrobial activity within category Comment
Organic acids Formaldehyde* Formic acid* - C Acetic acid* - C2 Propionic acid* - C3 Butyric acid* - C4 MCFA C6 C8 C10 C12 Etheric oils Carvacrol Eugenol Thymol 2-3 1 2-3
Fo rm al de hy de
Fo rm al de hy de
Fy sa lF ee d
Layer feed is contaminated with 3,5 log of a wild strain Salmonella derived from contaminated soya. Source: Selko Laboratory 2010
Chemical
Heavy metal elements, dioxin, melamine Bacterial pathogens (Salmonella), molds (mycotoxin)
Pre-feeding
Microbial
Pre- and post-feeding
5
Salmonella - Feed & Farm are main risks
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MCFA more (cost-)effective than essential oils in vitro
control
13
Fysal Feed is effective in high fat raw materials & feed
Solution: Use innovative fysal feed
Organic acid can’t penetrate fat Salmonella not killed
Fat detergent
Fat
Salmonella Feed
Fat protects Salmonella against heat & chemicals!
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Comparison efficacy Fysal Feed & formaldehyde
3
新研究设施New facilities
Mexico Laboratory
4
Common contaminants related to safety
Contaminants Example Critical control points
Physical
Metals, plastics
Pre-feeding
Buffer capacity 2350 500 600 500 600 500 1000 250 143
Fat % 13,5% 18,0% 12,0% 3,0% 3,0% 0,50 % 1,0 % 1,0 % 2,0 %
a: J. Coma, Ph. D. Salmonella Control In Pork: Effect of Animal Nutrition and Feeding
Control of Salmonella in the gut
Intervention routes
Stop growth and kill Block adhesion to gut Control multiplication Prevent invasion enterocytes
9
Attacking the Achilles’ heel of Salmonella
b: © European Communities, 1995-2005 C: Selko Feed Additive laboratorium 7
Salmonella infection routing in poultry
Feed Water Animal Environment Oral uptake
2
泰高的全球研发中心Research Centres
Skretting Aquaculture Research Centre
Poultry and Rabbit Research Centre
Ruminant Research Centre
Nutreco Canada AgResearch
Food Research Centre
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Mannobiose
A coconut derived product with high β-1,4 mannobiose content Produced by selective hydrolysis Patented technology (Fuji Oil Co., Japan) High solubility Blocks Salmonella effectively Modulates the immune system → Prolonged effect in control of Salmonella
Stop growth and kill MCFA to disrupt membrane
Block adhesion Mannobiose to block binding of Salmonella to gut wall
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Intervention Route 1
Feed Raw materials - Inhibition growth of Salmonella
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Fy sa lF ee d
Intervention Route 2
Block adhesion of Salmonella to the gut
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Blocking Salmonella adhesion to enterocytes
Type 1 fimbriae binding to mannobiose
Intervention routes to inhibit growth and kill Salmonella Stop growth and kill Organic acids with high bacteriostatic properties to disturb metabolism Inhibit invasion Butyrate and MCFA to down-regulate virulence genes
β-1,4 mannobiose 18
Mannobiose blocks Salmonella effectively in vitro
Benchmark mannose, mannobiose, mannan sources
1. 2. 3. 4. 5. Small intestinal enterocytes (pig) cultured in microwell plates Addition of test substance to medium Addition of Salmonella Incubation for x minutes Remove medium and measure Salmonella adhesion to enterocytes
Salmonella reduction in contaminated layer feed
3,50 Log Salmonella reduction 3,00 2,50 2,00 1,50 1,00 0,50 0,00
0, 1% 0, 2% 0, 6% 0, 8%
Fysal Feed is almost as effective as the maximum tolerated dose (660 ppm) of a 30% formaldehyde derived product.
FEED Salmonella-contaminated feed Non-pelletised feed High buffering capacity feed efficacy acids ↓ High fat content protecting salmonella Critical raw materials Meat-bone meal/fish meal Soybean meal Canola meal Risk expeller meal > solvent meal
1
Outline
Nutreco R & D in brief 泰高研发简介 R & D versus critical control points 研发与安全的关键环节 Salmonella control project 沙门氏杆菌的控制 Mold and mycotoxin control 霉菌与其毒素的控制 Enzymes and pathogen safety 酶与病原菌控制 Summary
* Or salts thereof
1 2 3 4 5
Benchmark for decontamination Formic acid or formate best Salmonella killer in vitro. Acetic acid promotes virulence of Salmonella (not desired). Butyric acid down-regulates virulance (desired).