强、弱筋小麦籽粒形成期蔗糖、淀粉合成相关酶活性及其与氮代谢的关系
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作物学报 ACTA AGRONOMICA SINICA 2008, 34(6): 1019-1026 ISSN 0496-3490; CODEN TSHPA9
http://www.chinacrops.org/zwxb/ E-mail: xbzw@chinajournal.netபைடு நூலகம்cn
DOI: 10.3724/SP.J.1006.2008.01019
Abstract: The strong- and weak-gluten cultivars are two typical patterns of high-quality wheat for different end-uses, between
which many differences exist in the synthesis, accumulation, concentration, and components of both protein and starch in grain. The experiment was conducted at Tai’an experimental station of Shandong Agricultural University in 2005-2006 growth season. A strong-gluten cultivar Gaocheng 8901 and a weak-gluten cultivar Yumai 50 were used to compare the contents of NO3--N, NH4+-N and activities of enzymes relating to nitrogen metabolism, sucrose and starch syntheses and the starch accumulation during grain filling, and to characterize the relationship between nitrogen and carbon metabolisms during grain development. The results indicated that the activities of sucrose synthase (SS), sucrose-phosphate synthase (SPS), adenosine diphosphorate glucose pyrophrylase (AGPP), soluble starch synthase (SSS), granule-bound starch synthase (GBSS), and starch branching enzyme (SBE)
Activities of Enzymes Involved in Sucrose and Starch Synthesis during Grain Filling and the Relation to Nitrogen Metabolism in Strong- and Weak-Gluten Wheat Cultivars
LI Jian-Min, WANG Zhen-Lin, GAO Rong-Qi, LI Sheng-Fu, CAI Rui-Guo, YAN Su-Hui, YU An-Ling, and YIN Yan-Ping*
(National Key Laboratory of Crop Biology, Agronomy College of Shandong Agricultural University, Tai’an 271018, Shandong, China)
强、弱筋小麦籽粒形成期蔗糖、淀粉合成相关酶活性及其与氮代谢的 关系
李建敏 王振林 高荣岐 李圣福 蔡瑞国 闫素辉 于安玲 尹燕枰*
(山东农业大学农学院作物生物学国家重点实验室, 山东泰安 271018)
摘 要: 2005—2006 年生长季, 以小麦强筋品种藁城 8901 和弱筋品种豫麦 50 为材料, 研究了灌浆期籽粒和旗叶氮代 谢底物含量与相关酶活性变化、蔗糖淀粉合成相关酶活性及籽粒淀粉积累特征, 分析了氮代谢与籽粒淀粉积累的关 系。结果表明, 旗叶蔗糖合酶(SS)和磷酸蔗糖合酶(SPS)、籽粒腺苷二磷酸葡萄糖焦磷酸化酶(AGPP)、可溶性淀粉合 酶(SSS)、束缚态淀粉合酶(GBSS)和淀粉分支酶(SBE)活性均呈单峰曲线变化。两品种比较, 除AGPP活性峰值豫麦 50 低于藁城 8901 外, 其他酶活性峰值均是豫麦 50 高于藁城 8901。相关分析表明, 藁城 8901 支链淀粉积累速率与SS、 SPS、AGPP和SBE活性呈极显著正相关, 相关系数分别为 0.9377**、0.8857**、0.6489**和 0.5980**; 直链淀粉积累速 率与SS、SPS活性呈极显著正相关, 相关系数分别为 0.7616**和 0.7750**。豫麦 50 支链淀粉积累速率与SS、SPS、AGPP、 GBSS和SBE活性呈极显著或显著正相关, 相关系数分别为 0.8182**、0.6762**、0.7028**、0.8749**和 0.5433*; 直链淀 粉积累速率与SS、SPS和SBE活性呈极显著正相关, 相关系数分别为 0.8528**、0.8428**和 0.8603**。两品种硝酸还原 酶(NR)、谷氨酰胺合成酶(GS)活性在开花后逐渐降低, 且藁城 8901 的NR活性一直高于豫麦 50。硝态氮和氨态氮含 量与NR、GS活性呈极显著正相关; 藁城 8901 的NR、GS活性与SS、SPS活性呈显著负相关, 而豫麦 50 只有GS与SPS 达显著负相关(r = -0.5212*)。上述结果表明, 不同品质类型小麦籽粒淀粉合成积累受氮代谢相关酶活性的影响, 藁城 8901 较高的NR活性抑制与蔗糖合成有关酶SS、SPS的活性, 导致淀粉合成积累速率降低。由此可见, SPS/NR比值对 淀粉合成具有重要调节作用。 关键词: 冬小麦; 强筋; 弱筋; 氮代谢; 淀粉; 酶活性
http://www.chinacrops.org/zwxb/ E-mail: xbzw@chinajournal.netபைடு நூலகம்cn
DOI: 10.3724/SP.J.1006.2008.01019
Abstract: The strong- and weak-gluten cultivars are two typical patterns of high-quality wheat for different end-uses, between
which many differences exist in the synthesis, accumulation, concentration, and components of both protein and starch in grain. The experiment was conducted at Tai’an experimental station of Shandong Agricultural University in 2005-2006 growth season. A strong-gluten cultivar Gaocheng 8901 and a weak-gluten cultivar Yumai 50 were used to compare the contents of NO3--N, NH4+-N and activities of enzymes relating to nitrogen metabolism, sucrose and starch syntheses and the starch accumulation during grain filling, and to characterize the relationship between nitrogen and carbon metabolisms during grain development. The results indicated that the activities of sucrose synthase (SS), sucrose-phosphate synthase (SPS), adenosine diphosphorate glucose pyrophrylase (AGPP), soluble starch synthase (SSS), granule-bound starch synthase (GBSS), and starch branching enzyme (SBE)
Activities of Enzymes Involved in Sucrose and Starch Synthesis during Grain Filling and the Relation to Nitrogen Metabolism in Strong- and Weak-Gluten Wheat Cultivars
LI Jian-Min, WANG Zhen-Lin, GAO Rong-Qi, LI Sheng-Fu, CAI Rui-Guo, YAN Su-Hui, YU An-Ling, and YIN Yan-Ping*
(National Key Laboratory of Crop Biology, Agronomy College of Shandong Agricultural University, Tai’an 271018, Shandong, China)
强、弱筋小麦籽粒形成期蔗糖、淀粉合成相关酶活性及其与氮代谢的 关系
李建敏 王振林 高荣岐 李圣福 蔡瑞国 闫素辉 于安玲 尹燕枰*
(山东农业大学农学院作物生物学国家重点实验室, 山东泰安 271018)
摘 要: 2005—2006 年生长季, 以小麦强筋品种藁城 8901 和弱筋品种豫麦 50 为材料, 研究了灌浆期籽粒和旗叶氮代 谢底物含量与相关酶活性变化、蔗糖淀粉合成相关酶活性及籽粒淀粉积累特征, 分析了氮代谢与籽粒淀粉积累的关 系。结果表明, 旗叶蔗糖合酶(SS)和磷酸蔗糖合酶(SPS)、籽粒腺苷二磷酸葡萄糖焦磷酸化酶(AGPP)、可溶性淀粉合 酶(SSS)、束缚态淀粉合酶(GBSS)和淀粉分支酶(SBE)活性均呈单峰曲线变化。两品种比较, 除AGPP活性峰值豫麦 50 低于藁城 8901 外, 其他酶活性峰值均是豫麦 50 高于藁城 8901。相关分析表明, 藁城 8901 支链淀粉积累速率与SS、 SPS、AGPP和SBE活性呈极显著正相关, 相关系数分别为 0.9377**、0.8857**、0.6489**和 0.5980**; 直链淀粉积累速 率与SS、SPS活性呈极显著正相关, 相关系数分别为 0.7616**和 0.7750**。豫麦 50 支链淀粉积累速率与SS、SPS、AGPP、 GBSS和SBE活性呈极显著或显著正相关, 相关系数分别为 0.8182**、0.6762**、0.7028**、0.8749**和 0.5433*; 直链淀 粉积累速率与SS、SPS和SBE活性呈极显著正相关, 相关系数分别为 0.8528**、0.8428**和 0.8603**。两品种硝酸还原 酶(NR)、谷氨酰胺合成酶(GS)活性在开花后逐渐降低, 且藁城 8901 的NR活性一直高于豫麦 50。硝态氮和氨态氮含 量与NR、GS活性呈极显著正相关; 藁城 8901 的NR、GS活性与SS、SPS活性呈显著负相关, 而豫麦 50 只有GS与SPS 达显著负相关(r = -0.5212*)。上述结果表明, 不同品质类型小麦籽粒淀粉合成积累受氮代谢相关酶活性的影响, 藁城 8901 较高的NR活性抑制与蔗糖合成有关酶SS、SPS的活性, 导致淀粉合成积累速率降低。由此可见, SPS/NR比值对 淀粉合成具有重要调节作用。 关键词: 冬小麦; 强筋; 弱筋; 氮代谢; 淀粉; 酶活性