组蛋白甲基转移酶活性or抑制分析试剂盒(H3K27)

EpiQuik组蛋白甲基转移酶活性/抑制检
测试剂盒（H3K27）
EpiQuik™ Histone Methyltransferase Activity/Inhibition Assay Kit (H3-K27)
产品名称：EpiQuik组蛋白甲基转移酶活性/抑制检测分析试剂盒（H3K27），EpiQuik™ Histone Methyltransferase Activity/Inhibition Assay Kit (H3K27)
产品货号：P-3005
规格：48次、96次分析
产品简介：
EpiQuik组蛋白甲基转移酶活性/抑制检测试剂盒（H3K27）是方便实验人员专门检测组蛋白H3第27位赖氨酸(H3-K27)的组蛋白甲基转移酶活性/抑制分析的一整套工具。

该试剂盒即时使用，提供全部必需的试剂用于成功进行组蛋白甲基转移酶活性/抑制性试验，不接触放射性物质或任何特殊设备（启维益成有售）。

这个试剂盒具有如下优点：
●快速操作，3小时内可完成。

●安全独创的比色测定，不接触放射性物质，无需提取和层析分析。

●专一性检测H3-K27组蛋白甲基转移酶活性/抑制。

●可剥离的微孔板让实验人员灵活选择手工或高通量分析。

●极其简便，结果可靠，一致的分析条件。

背景信息：
EpiQuik组蛋白甲基转移酶活性/抑制检测试剂盒（H3K27）是为了专门检测组蛋白H3第27位赖氨酸处组蛋白甲基转移酶活性而设计的。

在该试剂盒在实际检测中，组蛋白底物紧密结合在微孔表面，组蛋白甲基化转移酶把腺苷基蛋氨酸（Adomet）的一个甲基基团转移到组蛋白H3基质的第27个赖氨酸上将其甲基化。

甲基化的H3-K27能被一种高亲和性抗体识别，其数量与组蛋白甲基转移酶活性成线性关系，并且可通过HRP偶联的二抗显色系统进行定量。

因此，组蛋白甲基转移酶活性可以依据其所转换的甲基化H3-K27的数量进行计算（启维益成有售）。

试剂盒组分：
HM1 (10X 冲洗缓冲液)
HM2 (组蛋白检测缓冲液)
HM3 (腺苷基蛋氨酸)*
HM4 (生物素标记的底物, 25 µg/ml)*
HM5 (组蛋白甲基转移酶标准品, 10 µg/ml)*
HM6 (结合抗体, 100 µg/ml)*
HM7 (检测抗体, 200 µg/ml)*
HM8 (显色溶液)
HM9 (反应终止溶液)
对照酶样品(100 µg/ml)*
8孔检测板(带96孔框架)
产品应用案例：
Veazey KJ et. al. (September 2015). Dose-dependent alcohol-induced alterations in chromatin structure persist beyond the window of exposure and correlate with fetal alcohol syndrome birth defects. Epigenetics Chromatin. 8:39.
Zhang J et. al. (September 2015). Disruption of KMT2D perturbs germinal center B cell development and promotes lymphomagenesis. Nat Med.
Wan J et. al. (April 2015). PCAF-primed EZH2 acetylation regulates its stability and promotes lung adenocarcinoma progression. Nucleic Acids Res. 43(7):3591-604.
De la Cruz-Hernandez E et. al. (February 2015). Ribavirin as a tri-targeted antitumor repositioned drug. Oncol Rep.
Liu J et. al. (January 2015). Chromatin landscape defined by repressive histone methylation during oligodendrocyte differentiation. J Neurosci. 35(1):352-65.
Morishita M et. al. (December 2014). In vitro histone lysine methylation by NSD1, NSD2/MMSET/WHSC1, and NSD3/WHSC1L. BMC Struct Biol. 14(1):25.
Malmgren S et. al. (April 2013). Coordinate changes in histone modifications, mRNA levels, and metabolite profiles in clonal INS-1 832/13 β-cells accompany functional adaptations to lipotoxicity. J Biol Chem. 288(17):11973-87.
Latrasse D et. al. (March 2013). Dual function of MIPS1 as a metabolic enzyme and transcriptional regulator. Nucleic Acids Res. 41(5):2907-17.
Li Y et. al. (January 2013). Epigenetic regulation of multiple tumor-related genes leads to suppression of breast tumorigenesis by dietary genistein. PLoS One. 8(1):e54369.
Batra V et. al. (March 2012). Interaction between γ-radiation and dietary folate starvation metabolically reprograms global hepatic histone H3 methylation at lysine 4 and lysine 27 residues. Food Chem Toxicol. 50(3-4):464-72.
Rugg-Gunn PJ et. al. (June 2010). Distinct histone modifications in stem cell lines and tissue lineages from the early mouse embryo. Proc Natl Acad Sci U S A. 107(24):10783-90.
Nakade K et. al. (April 2009). JDP2 (Jun Dimerization Protein 2)-deficient mouse embryonic
fibroblasts are resistant to replicative senescence. J Biol Chem. 284(16):10808-17.。